# # Obtain gene names for transcripts with the biomaRt package. # # https://bioconductor.org/packages/release/bioc/html/biomaRt.html # # Load the biomart manager library(biomaRt) # The biomaRt dataset name. dataset <- "drerio_gene_ensembl" # The ouput file name. output_file = "tx2gene.csv" # Make a connection to the validated dataset. mart <- useEnsembl(dataset = dataset, biomart = 'ensembl') # The attributes that we want to obtain. # The first column must match the feature id used during quantification. attributes <- c( "ensembl_transcript_id_version", "ensembl_gene_id", "ensembl_transcript_id", "transcript_length", "external_gene_name" ) # Perform the query. data <- biomaRt::getBM(attributes = attributes, mart = mart) # Save the data into a file. write.csv(data, file=output_file, row.names=FALSE, quote=FALSE) # Inform the user. print("# Tool: Create tx2gene mapping") print(paste("# Dataset: ", dataset)) print(paste("# Output: ", output_file))