---
dataset_info:
  features:
  - name: image
    dtype: image
  - name: source
    dtype: string
  splits:
  - name: train
    num_bytes: 366173592
    num_examples: 120
  download_size: 366186996
  dataset_size: 366173592
configs:
- config_name: default
  data_files:
  - split: train
    path: data/train-*
task_categories:
- mask-generation
size_categories:
- n<1K
---
# Dataset Card

##  Dataset Summary  
The dataset contains fluorescence microscopy images of cells stained with **DAPI** (Which stains the nucleus, channel 1) and **CyclinB1** (which stains cytoplasm and nucleus, if it stains the nucleus it indicates the cell is in G2 or early prophase, channel 3). The goal of this project is to analyze whether Cyclin B1 is in the nucleus to determine where the cell is in the cell cycle.  

Each cell has two corresponding TIFF images:
- A **DAPI** image showing the nucleus  
- A **CyclinB1** image showing cell cycle protein levels  

The dataset was created for OPALS Group 19 project focused on understanding how CyclinB1 shows where the cell is in the cell cycle.



## Data Fields  
- **image** — A PIL Image
- **source** — The source of the data

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##  Data Collection  
Images were collected using fluorescence microscopy.  
Cells were stained with:
- **DAPI** to visualize nuclei  
- **CyclinB1** to measure cell cycle progression  

The dataset was prepared manually by organizing images and creating a JSON metadata file linking each cell’s channels.

---

## Intended Uses  
This dataset can be used for:

- Cell segmentation  
- Cell cycle classification  
- Training or testing microscopy image models  
- Educational demonstrations of fluorescence imaging  

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##  Limitations  
- Small dataset size (fewer than 1,000 samples)  
- Only two fluorescence channels  
- No segmentation masks included  
- Images may vary slightly in brightness or focus  

---

##  Citation  
If you use this dataset, please cite the dataset creator or link to the Hugging Face dataset page.