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  ## Usage Notes
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  - The **internal index** (`idx`) is the key that ties together:
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  - build training or evaluation sets for ligand–target or ligand–domain prediction,
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  - separate high-confidence from ambiguous associations,
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  - benchmark methods that infer protein targets from ligand similarity.
 
 
 
 
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+ ### 4. Organism-Specific Datasets
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+
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+ ReverseLigQ integrates multiple organisms, each identified by an integer key.
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+ The files in this section provide organism-level ligand lists, protein descriptions, and Pfam-based protein families.
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+ Organism keys and corresponding species:
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+ | Key | Organism |
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+ |-----|----------|
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+ | 1 | *Bartonella bacilliformis* |
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+ | 2 | *Klebsiella pneumoniae* |
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+ | 3 | *Mycobacterium tuberculosis* |
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+ | 4 | *Trypanosoma cruzi* |
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+ | 5 | *Staphylococcus aureus* RF122 |
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+ | 6 | *Streptococcus uberis* 0140J |
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+ | 7 | *Enterococcus faecium* |
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+ | 8 | *Escherichia coli* MG1655 |
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+ | 9 | *Streptococcus agalactiae* NEM316 |
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+ | 10 | *Pseudomonas syringae* |
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+ | 11 | DENV (Dengue virus) |
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+ | 12 | SARS-CoV-2 |
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+ | 13 | *Homo sapiens* |
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+
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+ #### Files:
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+ - **`ligand_lists.pkl`**
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+ Python dictionary containing the **list of ligands associated with each organism** included in the platform.
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+ Keys correspond to organism indices (1–13), and values are lists of ligand identifiers.
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+
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+ - **`prot_descriptions.pkl`**
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+ Python dictionary containing **protein descriptions** (annotations, names, functional summaries, etc.) for each organism.
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+ Keys are the organism indices listed above.
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+
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+ - **`fam_prot_dict.pkl`**
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+ Python dictionary mapping each organism to its **proteins grouped by Pfam families**.
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+ For each organism key, the value is another dictionary where:
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+ - keys are Pfam domain identifiers, and
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+ - values are lists of proteins belonging to that domain family.
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+ These files enable ReverseLigQ to perform organism-specific ligand searches, identify relevant targets, and reconstruct ligand–domain landscapes across diverse pathogens and host species.
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+ ---
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+
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  ## Usage Notes
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  - The **internal index** (`idx`) is the key that ties together:
 
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  - build training or evaluation sets for ligand–target or ligand–domain prediction,
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  - separate high-confidence from ambiguous associations,
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  - benchmark methods that infer protein targets from ligand similarity.
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+ - The organism-level dictionaries (`ligand_lists.pkl`, `prot_descriptions.pkl`, `fam_prot_dict.pkl`) support:
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+ - organism-specific prioritization of targets,
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+ - analyses of Pfam family distribution across species,
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+ - integration of ligand knowledge with genomic and proteomic data.
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