{
  "dataset_id": "27_microscopy_techniques",
  "title": "Microscopy Technique Reference",
  "domain": "analytical_instrumentation",
  "primary_entity_field": "technique",
  "field_guide": {
    "technique": "Technique",
    "typical_resolution_nm": "Typical Resolution (nm)",
    "sample_requirement": "Sample Requirement",
    "imaging_signal": "Imaging Signal",
    "common_application": "Common Application",
    "live_cell_friendly": "Live Cell Friendly"
  },
  "record_count": 6,
  "records": [
    {
      "record_id": "27_microscopy_techniques_01",
      "technique": "Bright-field microscopy",
      "typical_resolution_nm": 200,
      "sample_requirement": "Thin specimen, often stained",
      "imaging_signal": "Transmitted visible light",
      "common_application": "Histology overview",
      "live_cell_friendly": "Yes",
      "entity_name": "Bright-field microscopy",
      "summary": "Bright-field microscopy appears in the Microscopy Technique Reference dataset with technique=Bright-field microscopy; typical_resolution_nm=200; sample_requirement=Thin specimen, often stained; imaging_signal=Transmitted visible light; common_application=Histology overview; live_cell_friendly=Yes."
    },
    {
      "record_id": "27_microscopy_techniques_02",
      "technique": "Fluorescence microscopy",
      "typical_resolution_nm": 180,
      "sample_requirement": "Fluorescent labels or autofluorescence",
      "imaging_signal": "Emitted light",
      "common_application": "Protein localization",
      "live_cell_friendly": "Yes",
      "entity_name": "Fluorescence microscopy",
      "summary": "Fluorescence microscopy appears in the Microscopy Technique Reference dataset with technique=Fluorescence microscopy; typical_resolution_nm=180; sample_requirement=Fluorescent labels or autofluorescence; imaging_signal=Emitted light; common_application=Protein localization; live_cell_friendly=Yes."
    },
    {
      "record_id": "27_microscopy_techniques_03",
      "technique": "Confocal microscopy",
      "typical_resolution_nm": 120,
      "sample_requirement": "Fluorescent labeling",
      "imaging_signal": "Laser-scanned fluorescence",
      "common_application": "Optical sectioning and 3D imaging",
      "live_cell_friendly": "Yes",
      "entity_name": "Confocal microscopy",
      "summary": "Confocal microscopy appears in the Microscopy Technique Reference dataset with technique=Confocal microscopy; typical_resolution_nm=120; sample_requirement=Fluorescent labeling; imaging_signal=Laser-scanned fluorescence; common_application=Optical sectioning and 3D imaging; live_cell_friendly=Yes."
    },
    {
      "record_id": "27_microscopy_techniques_04",
      "technique": "Scanning electron microscopy",
      "typical_resolution_nm": 1.0,
      "sample_requirement": "Vacuum-compatible conductive surface",
      "imaging_signal": "Secondary or backscattered electrons",
      "common_application": "Surface topology",
      "live_cell_friendly": "No",
      "entity_name": "Scanning electron microscopy",
      "summary": "Scanning electron microscopy appears in the Microscopy Technique Reference dataset with technique=Scanning electron microscopy; typical_resolution_nm=1.0; sample_requirement=Vacuum-compatible conductive surface; imaging_signal=Secondary or backscattered electrons; common_application=Surface topology; live_cell_friendly=No."
    },
    {
      "record_id": "27_microscopy_techniques_05",
      "technique": "Transmission electron microscopy",
      "typical_resolution_nm": 0.1,
      "sample_requirement": "Ultrathin specimen in vacuum",
      "imaging_signal": "Transmitted electrons",
      "common_application": "Internal ultrastructure",
      "live_cell_friendly": "No",
      "entity_name": "Transmission electron microscopy",
      "summary": "Transmission electron microscopy appears in the Microscopy Technique Reference dataset with technique=Transmission electron microscopy; typical_resolution_nm=0.1; sample_requirement=Ultrathin specimen in vacuum; imaging_signal=Transmitted electrons; common_application=Internal ultrastructure; live_cell_friendly=No."
    },
    {
      "record_id": "27_microscopy_techniques_06",
      "technique": "Atomic force microscopy",
      "typical_resolution_nm": 1.0,
      "sample_requirement": "Surface accessible to probe tip",
      "imaging_signal": "Cantilever deflection",
      "common_application": "Nanoscale surface mapping",
      "live_cell_friendly": "Limited",
      "entity_name": "Atomic force microscopy",
      "summary": "Atomic force microscopy appears in the Microscopy Technique Reference dataset with technique=Atomic force microscopy; typical_resolution_nm=1.0; sample_requirement=Surface accessible to probe tip; imaging_signal=Cantilever deflection; common_application=Nanoscale surface mapping; live_cell_friendly=Limited."
    }
  ]
}