#!/usr/bin/env python3 """ V3Cell conditional inference script. Given the first n frames of an organoid video, predict the full developmental trajectory using a trained V3CellGenerator checkpoint. Usage: python scripts/inference.py \\ --checkpoint checkpoints/outputs/v3cell/checkpoint_002000.pt \\ --r3gan_ckpt checkpoints/training-runs/00002-frames-256-gpus3-batch96/network-snapshot-000000580.pkl \\ --test_dir datasets/vedio_8x \\ --output_dir outputs/inference_results \\ --n_condition_frames 6 \\ --gpu 0 """ import argparse import sys from pathlib import Path from typing import Dict, List, Tuple import numpy as np project_root = Path(__file__).parent.parent.resolve() sys.path.insert(0, str(project_root)) import torch from PIL import Image from tqdm import tqdm from models import V3CellGenerator from utils.misc import save_video_as_gif def parse_args(): parser = argparse.ArgumentParser(description='V3Cell Conditional Inference') parser.add_argument('--checkpoint', type=str, required=True, help='Path to trained checkpoint') parser.add_argument('--r3gan_ckpt', type=str, required=True, help='Path to R3GAN checkpoint') parser.add_argument('--test_dir', type=str, required=True, help='Path to test data directory') parser.add_argument('--output_dir', type=str, default='outputs/inference_results', help='Output directory') parser.add_argument('--n_condition_frames', type=int, default=6, help='Number of condition frames') parser.add_argument('--total_frames', type=int, default=25, help='Total frames to generate') parser.add_argument('--gpu', type=str, default='0', help='GPU id') parser.add_argument('--img_resolution', type=int, default=256, help='Image resolution') parser.add_argument('--save_individual_frames', action='store_true', help='Save individual frames as PNG') parser.add_argument('--classes', nargs='+', default=['non_lumen', 'lumen', 'lumen_collapse'], choices=['lumen', 'lumen_collapse', 'non_lumen'], help='Class folders to process') # Architecture flags — must match the settings used to train --checkpoint # (see scripts/train_conditional.py, which defaults to content-motion # separation with content_dim=32). parser.add_argument('--use_content_motion', action='store_true', default=True, help='Build the generator with MoCoGAN-style content/motion latent separation') parser.add_argument('--no_content_motion', action='store_true', help='Disable content-motion separation (use a single shared z_dim)') parser.add_argument('--content_dim', type=int, default=32, help='Content latent dimension (only used if content-motion separation is enabled)') return parser.parse_args() def load_model(checkpoint_path: str, r3gan_ckpt: str, device: torch.device, use_content_motion: bool = True, content_dim: int = 32) -> V3CellGenerator: """Load a trained V3CellGenerator from a checkpoint file.""" print(f"Loading checkpoint from: {checkpoint_path}") ckpt = torch.load(checkpoint_path, map_location=device) # Default model configuration (used if not stored in the checkpoint) z_dim = 64 c_dim = 3 img_resolution = 256 motion_hidden_dim = 384 motion_num_layers = 2 motion_dropout = 0.1 # Create model generator = V3CellGenerator( r3gan_checkpoint=r3gan_ckpt, z_dim=z_dim, c_dim=c_dim, img_resolution=img_resolution, motion_hidden_dim=motion_hidden_dim, motion_num_layers=motion_num_layers, motion_dropout=motion_dropout, residual_weight=0.2, use_content_motion=use_content_motion, content_dim=content_dim, use_encoder=True, freeze_r3gan=True, ) generator = generator.to(device) # Load trainable weights (MotionRNN + Encoder only; R3GAN is frozen) generator.load_trainable_state_dict(ckpt['generator']) generator.eval() print(f"Model loaded successfully!") print(f" Content-Motion separation: {use_content_motion}") print(f" Content dim: {content_dim}, Motion dim: {z_dim - content_dim}") return generator def load_video_frames(video_dir: Path, img_resolution: int = 256) -> Tuple[torch.Tensor, int]: """ Load all frames from a directory, using the same preprocessing as training. Returns: frames: [T, C, H, W] tensor normalised to [-1, 1] total_frames: number of frames loaded """ # Find all frame files frame_files = sorted(video_dir.glob("*.png")) if len(frame_files) == 0: raise ValueError(f"No frames found in {video_dir}") # Load and preprocess following the training procedure frames = [] for frame_file in frame_files: img = Image.open(frame_file).convert('RGB') # Resize to match training resolution if img.size != (img_resolution, img_resolution): img = img.resize( (img_resolution, img_resolution), Image.BILINEAR # Same interpolation method as training ) # Convert to numpy array frame = np.array(img) # [H, W, C], uint8 in [0, 255] frames.append(frame) frames = np.stack(frames, axis=0) # [T, H, W, C] # Convert to tensor (identical to training preprocessing) frames = frames.copy() # Ensure contiguous memory frames = torch.from_numpy(frames).float() frames = frames.permute(0, 3, 1, 2) # [T, C, H, W] frames = frames / 127.5 - 1.0 # Normalise to [-1, 1] (identical to training) return frames, len(frame_files) def get_class_label(class_name: str) -> torch.Tensor: """Return the one-hot class label (must match the mapping used during training).""" # Must match the mapping in data/video_dataset.py class_map = { 'lumen': 0, 'lumen_collapse': 1, 'non_lumen': 2, } if class_name not in class_map: raise ValueError(f"Unknown class: {class_name}") label = torch.zeros(3) label[class_map[class_name]] = 1.0 return label @torch.no_grad() def inference_single_video( generator: V3CellGenerator, video_frames: torch.Tensor, class_label: torch.Tensor, n_condition_frames: int, total_frames: int, device: torch.device, ) -> Tuple[torch.Tensor, torch.Tensor]: """ Run V3Cell conditional prediction on a single video. Args: generator: Trained V3CellGenerator. video_frames: [T, C, H, W] input video frames in [-1, 1]. class_label: [c_dim] one-hot class label. n_condition_frames: Number of observed frames used as conditioning input. total_frames: Total number of frames to generate. device: Target device. Returns: condition_frames: [n_condition_frames, C, H, W] observed conditioning frames. predicted_video: [total_frames, C, H, W] predicted full trajectory. """ # Use the first n frames as conditioning input condition_frames = video_frames[:n_condition_frames] # [k, C, H, W] # Add batch dimension condition_frames_batch = condition_frames.unsqueeze(0).to(device) # [1, k, C, H, W] class_label_batch = class_label.unsqueeze(0).to(device) # [1, c_dim] # Generate video predicted_video = generator.predict_from_frames( early_frames=condition_frames_batch, c=class_label_batch, total_frames=total_frames, ) # [1, T, C, H, W] # Remove batch dimension predicted_video = predicted_video[0] # [T, C, H, W] return condition_frames, predicted_video def save_results( condition_frames: torch.Tensor, real_frames: torch.Tensor, predicted_frames: torch.Tensor, output_dir: Path, video_name: str, save_individual: bool = False, ): """ Save inference results as GIFs (and optionally individual PNG frames). Args: condition_frames: [k, C, H, W] conditioning frames in [-1, 1]. real_frames: [T, C, H, W] ground-truth frames in [-1, 1]. predicted_frames: [T, C, H, W] predicted frames in [-1, 1]. output_dir: Directory to write results into. video_name: Base name used for output files. save_individual: If True, also save each predicted frame as a PNG. """ output_dir.mkdir(parents=True, exist_ok=True) # Save as GIF (save_video_as_gif handles denormalisation automatically) save_video_as_gif( condition_frames, output_dir / f"{video_name}_condition.gif" ) save_video_as_gif( real_frames, output_dir / f"{video_name}_real.gif" ) save_video_as_gif( predicted_frames, output_dir / f"{video_name}_predicted.gif" ) # Save individual frames (optional) if save_individual: frames_dir = output_dir / f"{video_name}_frames" frames_dir.mkdir(exist_ok=True) # Denormalise: [-1, 1] → [0, 255] frames_denorm = (predicted_frames + 1.0) / 2.0 # → [0, 1] frames_denorm = (frames_denorm * 255).clamp(0, 255) for i, frame in enumerate(frames_denorm): frame_np = frame.permute(1, 2, 0).cpu().numpy().astype(np.uint8) img = Image.fromarray(frame_np) img.save(frames_dir / f"frame_{i:03d}.png") def main(): args = parse_args() # Set device device = torch.device(f'cuda:{args.gpu}' if torch.cuda.is_available() else 'cpu') print(f"Using device: {device}") # Load model use_content_motion = args.use_content_motion and not args.no_content_motion generator = load_model(args.checkpoint, args.r3gan_ckpt, device, use_content_motion=use_content_motion, content_dim=args.content_dim) # Test data directory test_dir = Path(args.test_dir) output_dir = Path(args.output_dir) if not test_dir.exists(): raise ValueError(f"Test directory not found: {test_dir}") print(f"\nTest data directory: {test_dir}") print(f"Output directory: {output_dir}") print(f"Condition frames: {args.n_condition_frames}") print(f"Total frames: {args.total_frames}") print() # Iterate over classes class_names = args.classes all_results = [] for class_name in class_names: class_dir = test_dir / class_name if not class_dir.exists(): print(f"Warning: Class directory not found: {class_dir}") continue print(f"\nProcessing class: {class_name}") print("=" * 60) # Get class label class_label = get_class_label(class_name) # Iterate over all videos in this class video_dirs = sorted([d for d in class_dir.iterdir() if d.is_dir()]) for video_dir in tqdm(video_dirs, desc=f" {class_name}"): video_name = video_dir.name try: # Load video frames video_frames, total_frames = load_video_frames(video_dir, args.img_resolution) if total_frames < args.n_condition_frames: print(f" Warning: {video_name} has only {total_frames} frames, skipping...") continue # Run inference condition_frames, predicted_video = inference_single_video( generator=generator, video_frames=video_frames, class_label=class_label, n_condition_frames=args.n_condition_frames, total_frames=args.total_frames, device=device, ) # Save results class_output_dir = output_dir / class_name save_results( condition_frames=condition_frames, real_frames=video_frames[:args.total_frames], predicted_frames=predicted_video, output_dir=class_output_dir, video_name=video_name, save_individual=args.save_individual_frames, ) all_results.append({ 'class': class_name, 'video': video_name, 'total_frames': total_frames, 'success': True, }) except Exception as e: print(f" Error processing {video_name}: {e}") all_results.append({ 'class': class_name, 'video': video_name, 'success': False, 'error': str(e), }) # Print summary statistics print("\n" + "=" * 60) print("Inference completed!") print("=" * 60) success_count = sum(1 for r in all_results if r['success']) total_count = len(all_results) print(f"Total videos: {total_count}") print(f"Success: {success_count}") print(f"Failed: {total_count - success_count}") print(f"\nResults saved to: {output_dir}") # Per-class breakdown for class_name in class_names: class_results = [r for r in all_results if r['class'] == class_name] if class_results: class_success = sum(1 for r in class_results if r['success']) print(f" {class_name}: {class_success}/{len(class_results)}") if __name__ == '__main__': main()