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The dataset generation failed because of a cast error
Error code: DatasetGenerationCastError
Exception: DatasetGenerationCastError
Message: An error occurred while generating the dataset
All the data files must have the same columns, but at some point there are 18 new columns ({' Variable_reactant ', ' Unit_Kobs ', 'km_kcat', 'km', ' Commentary[Others] ', ' Commentary[pH] ', 'kcat', ' Commentary[Mutant] ', 'Kcleav', ' Unit_Kcat/Km ', ' Commentary[Temp] ', ' Organism ', ' Unit_Kcleav ', 'Kobs', 'Source', ' Ribozyme ', ' Unit_Kcat ', ' Unit_Km '}) and 12 missing columns ({'km_kcat(mM^-1s^-1)', 'Commentary[Cosubstrate]', 'Commentary[pH]', 'Commentary[Mutant]', 'Enzyme', 'kcat(s^-1)', 'ID_gold', 'Commentary[Temp]', 'Organism', 'Substrate', 'Reference[Table/Text]', 'km(mM)'}).
This happened while the csv dataset builder was generating data using
hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark/20241213_Ribozyme_benchmark.csv (at revision fec27341c9fe117dbfb7756a065f5b6c72f3d630), [/tmp/hf-datasets-cache/medium/datasets/40258543484657-config-parquet-and-info-jackkuo-LLM-Enzyme-Kineti-f753539c/hub/datasets--jackkuo--LLM-Enzyme-Kinetics-Golden-Benchmark/snapshots/fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241212_Golden_Benchmark_for_Enzyme_Kinetics_converted.csv (origin=hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark@fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241212_Golden_Benchmark_for_Enzyme_Kinetics_converted.csv), /tmp/hf-datasets-cache/medium/datasets/40258543484657-config-parquet-and-info-jackkuo-LLM-Enzyme-Kineti-f753539c/hub/datasets--jackkuo--LLM-Enzyme-Kinetics-Golden-Benchmark/snapshots/fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241213_Ribozyme_benchmark.csv (origin=hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark@fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241213_Ribozyme_benchmark.csv)]
Please either edit the data files to have matching columns, or separate them into different configurations (see docs at https://hf.co/docs/hub/datasets-manual-configuration#multiple-configurations)
Traceback: Traceback (most recent call last):
File "/usr/local/lib/python3.12/site-packages/datasets/builder.py", line 1887, in _prepare_split_single
writer.write_table(table)
File "/usr/local/lib/python3.12/site-packages/datasets/arrow_writer.py", line 674, in write_table
pa_table = table_cast(pa_table, self._schema)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/usr/local/lib/python3.12/site-packages/datasets/table.py", line 2272, in table_cast
return cast_table_to_schema(table, schema)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/usr/local/lib/python3.12/site-packages/datasets/table.py", line 2218, in cast_table_to_schema
raise CastError(
datasets.table.CastError: Couldn't cast
Ribozyme : string
Organism : string
Variable_reactant : string
Kobs: string
Unit_Kobs : string
km: string
Unit_Km : string
kcat: string
Unit_Kcat : string
km_kcat: string
Unit_Kcat/Km : string
Kcleav: string
Unit_Kcleav : string
Commentary[Temp] : string
Commentary[pH] : string
Commentary[Mutant] : string
Commentary[Others] : string
pubmed_id: string
Source: string
-- schema metadata --
pandas: '{"index_columns": [{"kind": "range", "name": null, "start": 0, "' + 2580
to
{'ID_gold': Value('int64'), 'pubmed_id': Value('int64'), 'Enzyme': Value('string'), 'Organism': Value('string'), 'Substrate': Value('string'), 'km(mM)': Value('string'), 'kcat(s^-1)': Value('string'), 'km_kcat(mM^-1s^-1)': Value('string'), 'Commentary[Temp]': Value('string'), 'Commentary[pH]': Value('string'), 'Commentary[Mutant]': Value('string'), 'Commentary[Cosubstrate]': Value('string'), 'Reference[Table/Text]': Value('string')}
because column names don't match
During handling of the above exception, another exception occurred:
Traceback (most recent call last):
File "/src/services/worker/src/worker/job_runners/config/parquet_and_info.py", line 1347, in compute_config_parquet_and_info_response
parquet_operations = convert_to_parquet(builder)
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/src/services/worker/src/worker/job_runners/config/parquet_and_info.py", line 980, in convert_to_parquet
builder.download_and_prepare(
File "/usr/local/lib/python3.12/site-packages/datasets/builder.py", line 884, in download_and_prepare
self._download_and_prepare(
File "/usr/local/lib/python3.12/site-packages/datasets/builder.py", line 947, in _download_and_prepare
self._prepare_split(split_generator, **prepare_split_kwargs)
File "/usr/local/lib/python3.12/site-packages/datasets/builder.py", line 1736, in _prepare_split
for job_id, done, content in self._prepare_split_single(
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/usr/local/lib/python3.12/site-packages/datasets/builder.py", line 1889, in _prepare_split_single
raise DatasetGenerationCastError.from_cast_error(
datasets.exceptions.DatasetGenerationCastError: An error occurred while generating the dataset
All the data files must have the same columns, but at some point there are 18 new columns ({' Variable_reactant ', ' Unit_Kobs ', 'km_kcat', 'km', ' Commentary[Others] ', ' Commentary[pH] ', 'kcat', ' Commentary[Mutant] ', 'Kcleav', ' Unit_Kcat/Km ', ' Commentary[Temp] ', ' Organism ', ' Unit_Kcleav ', 'Kobs', 'Source', ' Ribozyme ', ' Unit_Kcat ', ' Unit_Km '}) and 12 missing columns ({'km_kcat(mM^-1s^-1)', 'Commentary[Cosubstrate]', 'Commentary[pH]', 'Commentary[Mutant]', 'Enzyme', 'kcat(s^-1)', 'ID_gold', 'Commentary[Temp]', 'Organism', 'Substrate', 'Reference[Table/Text]', 'km(mM)'}).
This happened while the csv dataset builder was generating data using
hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark/20241213_Ribozyme_benchmark.csv (at revision fec27341c9fe117dbfb7756a065f5b6c72f3d630), [/tmp/hf-datasets-cache/medium/datasets/40258543484657-config-parquet-and-info-jackkuo-LLM-Enzyme-Kineti-f753539c/hub/datasets--jackkuo--LLM-Enzyme-Kinetics-Golden-Benchmark/snapshots/fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241212_Golden_Benchmark_for_Enzyme_Kinetics_converted.csv (origin=hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark@fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241212_Golden_Benchmark_for_Enzyme_Kinetics_converted.csv), /tmp/hf-datasets-cache/medium/datasets/40258543484657-config-parquet-and-info-jackkuo-LLM-Enzyme-Kineti-f753539c/hub/datasets--jackkuo--LLM-Enzyme-Kinetics-Golden-Benchmark/snapshots/fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241213_Ribozyme_benchmark.csv (origin=hf://datasets/jackkuo/LLM-Enzyme-Kinetics-Golden-Benchmark@fec27341c9fe117dbfb7756a065f5b6c72f3d630/20241213_Ribozyme_benchmark.csv)]
Please either edit the data files to have matching columns, or separate them into different configurations (see docs at https://hf.co/docs/hub/datasets-manual-configuration#multiple-configurations)Need help to make the dataset viewer work? Make sure to review how to configure the dataset viewer, and open a discussion for direct support.
ID_gold
int64 | pubmed_id
int64 | Enzyme
string | Organism
string | Substrate
string | km(mM)
string | kcat(s^-1)
string | km_kcat(mM^-1s^-1)
string | Commentary[Temp]
string | Commentary[pH]
string | Commentary[Mutant]
string | Commentary[Cosubstrate]
string | Reference[Table/Text]
string |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Glc
|
13.5
|
34.4
|
2.58
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
2
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Gal
|
7.3
|
13.5
|
1.9
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
3
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Man
|
0.44
|
4.2
|
9.8
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
4
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Glc
|
16.2
|
25.5
|
1.6
|
90°C
|
4.75
|
Q77R
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
5
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Gal
|
16.8
|
14.2
|
0.85
|
90°C
|
4.75
|
Q77R
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
6
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Man
|
1.1
|
1.6
|
1.4
|
90°C
|
4.75
|
Q77R
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
7
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Glc
|
0.3
|
252
|
870
|
90°C
|
4.75
|
D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
8
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Gal
|
7.8
|
571
|
75.8
|
90°C
|
4.75
|
D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
9
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Man
|
3.2
|
45
|
14.7
|
90°C
|
4.75
|
D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
10
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Glc
|
30.4
|
439
|
14.4
|
90°C
|
4.75
|
Q77R/D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
11
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Gal
|
36.1
|
66.5
|
1.8
|
90°C
|
4.75
|
Q77R/D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
12
| 11,900,558
|
BglB
|
Pyrococcus horikoshii
|
pNP-Man
|
0.68
|
6.8
|
9.9
|
90°C
|
4.75
|
Q77R/D206N
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
13
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Glc
|
0.19
|
1140
|
7337
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
14
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Gal
|
5
|
2827
|
561
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
15
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man
|
1.3
|
65.9
|
49.8
|
90°C
|
4.75
|
WT
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
16
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Glc
|
13.8
|
207
|
14.9
|
90°C
|
4.75
|
R77Q
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
17
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Gal
|
21.7
|
69.2
|
3.2
|
90°C
|
4.75
|
R77Q
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
18
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man I
|
0.64
|
1.22
|
1.9
|
90°C
|
4.75
|
R77Q
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
19
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man II
|
0.15
|
0.85
|
5.7
|
90°C
|
4.75
|
R77Q
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
20
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Gal
|
15.7
|
180
|
11.5
|
90°C
|
4.75
|
N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
21
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Glc
|
4.6
|
116
|
24.9
|
90°C
|
4.75
|
N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
22
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man
|
0.63
|
1.97
|
3.1
|
90°C
|
4.75
|
N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
23
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Glc
|
13.9
|
172
|
12.3
|
90°C
|
4.75
|
R77Q/N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
24
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Gal
|
22
|
29.2
|
1.3
|
90°C
|
4.75
|
R77Q/N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
25
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man I
|
1.5
|
1.9
|
1.3
|
90°C
|
4.75
|
R77Q/N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
26
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man II
|
0.1
|
1.02
|
10.2
|
90°C
|
4.75
|
R77Q/N206D
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
27
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Glc
|
12
|
309
|
26
|
90°C
|
4.75
|
N206S
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
28
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Gal
|
200
|
1090
|
5.5
|
90°C
|
4.75
|
N206S
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
29
| 11,900,558
|
CelB
|
Pyrococcus furiosus
|
pNP-Man
|
4.7
|
15
|
3.2
|
90°C
|
4.75
|
N206S
|
0.1 M Sodium Citrate/0.1 M NaPi
|
Table
|
30
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0006
|
63.5
|
106000
|
25°C
|
6.0
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
31
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0011
|
86.2
|
75500
|
25°C
|
6.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
32
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0037
|
158.8
|
42700
|
25°C
|
7.0
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
33
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0071
|
293.4
|
40300
|
25°C
|
7.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
34
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.022
|
345.3
|
15300
|
25°C
|
8.0
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
35
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.086
|
410
|
4880
|
25°C
|
8.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
36
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.324
|
519
|
1500
|
25°C
|
9.0
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
37
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.66
|
431
|
251
|
25°C
|
9.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
38
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
3.389
|
23.7
|
6.77
|
25°C
|
10.0
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
39
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.004
|
36.8
|
8420
|
25°C
|
6.0
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
40
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.007
|
41.6
|
5600
|
25°C
|
6.5
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
41
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.026
|
51.8
|
1970
|
25°C
|
7.0
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
42
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.042
|
48.3
|
1140
|
25°C
|
7.5
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
43
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.114
|
53.4
|
461
|
25°C
|
8.0
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
44
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.332
|
58.3
|
173
|
25°C
|
8.5
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
45
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.155
|
64
|
54.5
|
25°C
|
9.0
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
46
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
4.456
|
52.7
|
11.6
|
25°C
|
9.5
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
47
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
15.487
|
44.8
|
2.84
|
25°C
|
10.0
|
Y236F
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
48
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.812
|
70.5
|
86.8
|
25°C
|
5.8
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
49
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.087
|
64
|
58.9
|
25°C
|
6.2
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
50
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.63
|
64
|
39.3
|
25°C
|
6.6
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
51
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.95
|
66.3
|
34
|
25°C
|
7.0
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
52
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
3.62
|
68.3
|
18.9
|
25°C
|
7.5
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
53
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
8.17
|
73.4
|
8.98
|
25°C
|
7.9
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
54
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
33.27
|
109.88
|
3.3
|
25°C
|
8.3
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
55
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.667
|
238.8
|
358
|
25°C
|
6.0
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
56
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.29
|
226.5
|
782
|
25°C
|
6.5
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
57
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.189
|
214.5
|
1130
|
25°C
|
7.0
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
58
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.22
|
220.5
|
1010
|
25°C
|
7.5
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
59
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.27
|
214.6
|
799
|
25°C
|
8.0
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
60
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.452
|
222.4
|
492
|
25°C
|
8.5
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
61
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.46
|
192.5
|
132
|
25°C
|
9.0
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
62
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
12.2
|
148.4
|
12.2
|
25°C
|
9.5
|
H57A
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
63
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0032
|
730
|
225000
|
10°C
|
6
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
64
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0036
|
847
|
239000
|
10°C
|
6.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
65
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0014
|
782
|
55800
|
10°C
|
7
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
66
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0031
|
677
|
21800
|
10°C
|
7.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
67
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.0087
|
776
|
8890
|
10°C
|
8
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
68
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
0.412
|
829
|
2010
|
10°C
|
8.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
69
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
1.183
|
731
|
618
|
10°C
|
9
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
70
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
3.811
|
674
|
177
|
10°C
|
9.5
|
WT
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
71
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
4.53
|
746
|
165
|
10°C
|
5.5
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
72
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
6.48
|
662
|
102
|
10°C
|
6
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
73
| 19,004,819
|
SDH
|
S. novella
|
sulfite
|
22.8
|
240
|
10.5
|
10°C
|
7
|
R55M
|
20 mM Tris acetate buffer,0.04 mM cytochrome c,sulfite dehydrogenase mM cytochrome c
|
Table
|
98
| 21,105,905
|
TCME1
|
Trypanosoma cruzi
|
L-Malate
|
0.13
|
518
|
3981
| null |
7.4
|
WT
|
NADP+(0.5), Mn2+(0.5)
|
Table
|
99
| 21,105,905
|
TCME1
|
Trypanosoma cruzi
|
NADP+
|
0.027
|
504
|
18681
| null |
7.4
|
WT
|
L-Malate (5), Mn2+(0.5)
|
Table
|
100
| 21,105,905
|
TCME1
|
Trypanosoma cruzi
|
Pyruvate
|
7.6
|
8.3
|
1.1
| null |
7.4
|
WT
|
NADPH(0.5), Mn2+(0.5), HCO3-(5)
|
Table
|
101
| 21,105,905
|
TCME1
|
Trypanosoma cruzi
|
Mg2+
|
0.04
|
461
|
11513
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
102
| 21,105,905
|
TCME1
|
Trypanosoma cruzi
|
Mn2+
|
0.0043
|
518
|
120361
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
103
| 21,105,905
|
TcME2
|
Trypanosoma cruzi
|
L-Malate
|
2.3
|
683
|
297
| null |
7.4
|
WT
|
NADP+(0.5), Mn2+(0.5)
|
Table
|
104
| 21,105,905
|
TcME2
|
Trypanosoma cruzi
|
NADP+
|
0.032
|
691
|
21599
| null |
7.4
|
WT
|
L-Malate (35), Mn2+(0.5)
|
Table
|
105
| 21,105,905
|
TcME2
|
Trypanosoma cruzi
|
Pyruvate
|
5.1
|
39
|
8
| null |
7.4
|
WT
|
NADPH(0.5), Mn2+(0.5), HCO3-(5)
|
Table
|
106
| 21,105,905
|
TcME2
|
Trypanosoma cruzi
|
Mg2+
|
0.57
|
864
|
1516
| null |
7.4
|
WT
|
L-Malate (35), NADP+(0.5)
|
Table
|
107
| 21,105,905
|
TcME2
|
Trypanosoma cruzi
|
Mn2+
|
0.0091
|
687
|
75479
| null |
7.4
|
WT
|
L-Malate (35), NADP+(0.5)
|
Table
|
108
| 21,105,905
|
TbME1
|
Trypanosoma brucei
|
L-Malate
|
0.32
|
1132
|
3536
| null |
7.4
|
WT
|
NADP+(0.5), Mn2+(0.5)
|
Table
|
109
| 21,105,905
|
TbME1
|
Trypanosoma brucei
|
NADP+
|
0.048
|
1206
|
25166
| null |
7.4
|
WT
|
L-Malate (5), Mn2+(0.5)
|
Table
|
110
| 21,105,905
|
TbME1
|
Trypanosoma brucei
|
Pyruvate
|
4.3
|
113
|
26
| null |
7.4
|
WT
|
NADPH(0.5), Mn2+(0.5), HCO3-(5)
|
Table
|
111
| 21,105,905
|
TbME1
|
Trypanosoma brucei
|
Mg2+
|
0.27
|
1280
|
4739
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
112
| 21,105,905
|
TbME1
|
Trypanosoma brucei
|
Mn2+
|
0.0024
|
905
|
377192
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
113
| 21,105,905
|
TbME2
|
Trypanosoma brucei
|
L-Malate
|
0.49
|
242
|
494
| null |
7.4
|
WT
|
NADP+(0.5), Mn2+(0.5)
|
Table
|
114
| 21,105,905
|
TbME2
|
Trypanosoma brucei
|
NADP+
|
0.016
|
284
|
17775
| null |
7.4
|
WT
|
L-Malate (5), Mn2+(0.5)
|
Table
|
115
| 21,105,905
|
TbME2
|
Trypanosoma brucei
|
Pyruvate
|
5.3
|
34
|
6
| null |
7.4
|
WT
|
NADPH(0.5), Mn2+(0.5), HCO3-(5)
|
Table
|
116
| 21,105,905
|
TbME2
|
Trypanosoma brucei
|
Mg2+
|
0.58
|
208
|
359
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
117
| 21,105,905
|
TbME2
|
Trypanosoma brucei
|
Mn2+
|
0.0066
|
246
|
37302
| null |
7.4
|
WT
|
L-Malate (5), NADP+(0.5)
|
Table
|
118
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.024
|
59.1
|
2460
|
Room temperature
|
7
|
WT
|
potassium phosphate buffer
|
Table
|
119
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
| null |
20.8
| null |
Room temperature
|
7
|
E16G
|
potassium phosphate buffer
|
Table
|
120
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.0167
|
3.17
|
190
|
Room temperature
|
7
|
R18G
|
potassium phosphate buffer
|
Table
|
121
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.0126
|
140
|
11110
|
Room temperature
|
7
|
R21G
|
potassium phosphate buffer
|
Table
|
122
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.0288
|
6.61
|
230
|
Room temperature
|
7
|
L59G
|
potassium phosphate buffer
|
Table
|
123
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.0335
|
21.1
|
630
|
Room temperature
|
7
|
W62A
|
potassium phosphate buffer
|
Table
|
124
| 22,387,379
|
AzoA
|
Enterococcus faecalis
|
Methyl Red
|
0.0053
|
77.3
|
14580
|
Room temperature
|
7
|
R66A
|
potassium phosphate buffer
|
Table
|
End of preview.