NegBioDB / src /negbiodb_depmap /ge_features.py
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NegBioDB final: 4 domains, fully audited
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"""Omics feature computation for GE domain ML models.
Features per gene-cell_line pair (~75 dimensions):
Gene-level (8 dims): essentiality profile stats, fraction essential,
common essential flag, reference nonessential flag, RNAi concordance
Cell line (variable): lineage one-hot, disease one-hot, mutation burden
Gene × Cell line omics (3 dims): expression TPM, copy number, mutation indicator
Omics files (OmicsExpression, OmicsCN, OmicsSomatic) are NOT stored in SQLite
(too large). They are loaded directly from CSV during feature computation.
"""
from __future__ import annotations
import logging
from pathlib import Path
import numpy as np
import pandas as pd
logger = logging.getLogger(__name__)
def compute_gene_features(conn) -> pd.DataFrame:
"""Compute gene-level aggregate features from gene_cell_pairs.
Returns DataFrame indexed by gene_id with columns:
mean_effect, std_effect, min_effect, max_effect,
fraction_essential, is_common_essential, is_reference_nonessential,
rnai_concordance_fraction
"""
# Essentiality profile across cell lines
query = """
SELECT
g.gene_id,
AVG(p.mean_gene_effect) as mean_effect,
CASE COUNT(p.pair_id) WHEN 1 THEN 0.0 ELSE
SQRT(SUM((p.mean_gene_effect - sub.gmean) * (p.mean_gene_effect - sub.gmean))
/ (COUNT(p.pair_id) - 1)) END as std_effect,
MIN(p.min_gene_effect) as min_effect,
MAX(p.max_gene_effect) as max_effect,
g.is_common_essential,
g.is_reference_nonessential,
p.gene_degree
FROM genes g
JOIN gene_cell_pairs p ON g.gene_id = p.gene_id
LEFT JOIN (
SELECT gene_id, AVG(mean_gene_effect) as gmean
FROM gene_cell_pairs GROUP BY gene_id
) sub ON g.gene_id = sub.gene_id
GROUP BY g.gene_id
"""
df = pd.read_sql_query(query, conn, index_col="gene_id")
# RNAi concordance: fraction of this gene's cell lines with multi-source support
rnai_query = """
SELECT gene_id,
CAST(SUM(CASE WHEN num_sources >= 2 THEN 1 ELSE 0 END) AS REAL) /
NULLIF(COUNT(*), 0) as rnai_concordance_fraction
FROM gene_cell_pairs
GROUP BY gene_id
"""
rnai_df = pd.read_sql_query(rnai_query, conn, index_col="gene_id")
df = df.join(rnai_df, how="left")
df["rnai_concordance_fraction"] = df["rnai_concordance_fraction"].fillna(0.0)
logger.info("Computed gene features for %d genes", len(df))
return df
def compute_cell_line_features(conn) -> pd.DataFrame:
"""Compute cell line features: lineage/disease one-hot + degree.
Returns DataFrame indexed by cell_line_id.
"""
query = """
SELECT cell_line_id, lineage, primary_disease
FROM cell_lines
"""
df = pd.read_sql_query(query, conn, index_col="cell_line_id")
# One-hot encode lineage
lineage_dummies = pd.get_dummies(df["lineage"], prefix="lineage", dtype=float)
disease_dummies = pd.get_dummies(df["primary_disease"], prefix="disease", dtype=float)
result = pd.concat([lineage_dummies, disease_dummies], axis=1)
result.index = df.index
# Cell line degree from gene_cell_pairs
degree_query = """
SELECT cell_line_id, cell_line_degree
FROM gene_cell_pairs
GROUP BY cell_line_id
"""
deg_df = pd.read_sql_query(degree_query, conn, index_col="cell_line_id")
result = result.join(deg_df, how="left")
result["cell_line_degree"] = result["cell_line_degree"].fillna(0)
logger.info("Computed cell line features: %d cell lines, %d dims", len(result), result.shape[1])
return result
def load_omics_features(
expression_file: Path | None = None,
cn_file: Path | None = None,
mutation_file: Path | None = None,
model_ids: list[str] | None = None,
gene_symbols: list[str] | None = None,
) -> dict[tuple[str, str], np.ndarray]:
"""Load per-gene-per-cell-line omics features from CSV files.
Each file is a wide matrix: rows = ModelID, columns = gene symbols.
Args:
expression_file: OmicsExpressionProteinCodingGenesTPMLogp1.csv
cn_file: OmicsCNGene.csv
mutation_file: OmicsSomaticMutationsMatrixDamaging.csv
model_ids: Filter to these model IDs (reduces memory).
gene_symbols: Filter to these gene symbols.
Returns:
Dict mapping (model_id, gene_symbol) → np.array([expression, cn, mutation]).
"""
features: dict[tuple[str, str], list[float]] = {}
def _load_matrix(filepath: Path, feat_idx: int) -> None:
"""Load a single omics matrix and populate features dict."""
df = pd.read_csv(filepath, index_col=0)
# Filter columns (gene symbols) — column headers may be "HUGO (EntrezID)"
if gene_symbols is not None:
# Try direct match first
matching_cols = [c for c in df.columns if c in gene_symbols]
# Also try stripping entrez suffix
if not matching_cols:
col_map = {}
for c in df.columns:
parts = c.split(" (")
if parts[0] in gene_symbols:
col_map[c] = parts[0]
if col_map:
df = df[list(col_map.keys())]
df.columns = [col_map[c] for c in df.columns]
else:
df = df[matching_cols]
# Filter rows (model IDs)
if model_ids is not None:
available = [m for m in model_ids if m in df.index]
df = df.loc[available]
for model_id in df.index:
mid = str(model_id).strip()
for col in df.columns:
gene = col.split(" (")[0] if " (" in col else col
key = (mid, gene)
if key not in features:
features[key] = [0.0, 0.0, 0.0]
val = df.at[model_id, col]
if not pd.isna(val):
features[key][feat_idx] = float(val)
if expression_file and expression_file.exists():
_load_matrix(expression_file, 0)
logger.info("Loaded expression features from %s", expression_file)
if cn_file and cn_file.exists():
_load_matrix(cn_file, 1)
logger.info("Loaded copy number features from %s", cn_file)
if mutation_file and mutation_file.exists():
_load_matrix(mutation_file, 2)
logger.info("Loaded mutation features from %s", mutation_file)
result = {k: np.array(v) for k, v in features.items()}
logger.info("Loaded omics features for %d (model, gene) pairs", len(result))
return result
def build_feature_matrix(
conn,
pairs_df: pd.DataFrame,
omics_features: dict[tuple[str, str], np.ndarray] | None = None,
) -> np.ndarray:
"""Build combined feature matrix for ML training.
Args:
conn: SQLite connection.
pairs_df: DataFrame with gene_id, cell_line_id, gene_symbol, model_id columns.
omics_features: Optional dict from load_omics_features.
Returns:
Feature matrix (n_samples × n_features).
"""
gene_feats = compute_gene_features(conn)
cl_feats = compute_cell_line_features(conn)
gene_cols = ["mean_effect", "std_effect", "min_effect", "max_effect",
"is_common_essential", "is_reference_nonessential",
"rnai_concordance_fraction", "gene_degree"]
cl_cols = list(cl_feats.columns)
rows = []
for _, pair in pairs_df.iterrows():
gid = pair["gene_id"]
clid = pair["cell_line_id"]
# Gene features
if gid in gene_feats.index:
g = gene_feats.loc[gid, gene_cols].values.astype(float)
else:
g = np.zeros(len(gene_cols))
# Cell line features
if clid in cl_feats.index:
c = cl_feats.loc[clid, cl_cols].values.astype(float)
else:
c = np.zeros(len(cl_cols))
# Omics features
if omics_features is not None:
gene_symbol = pair.get("gene_symbol", "")
model_id = pair.get("model_id", "")
omics = omics_features.get((model_id, gene_symbol), np.zeros(3))
else:
omics = np.zeros(3)
row = np.concatenate([g, c, omics])
rows.append(row)
X = np.stack(rows)
n_nan = np.isnan(X).sum()
if n_nan > 0:
logger.warning("Feature matrix has %d NaN values, replacing with 0", n_nan)
X = np.nan_to_num(X, nan=0.0)
logger.info("Feature matrix: %d samples × %d features", X.shape[0], X.shape[1])
return X