An extract of Euphorbia terracina L. has yielded six acylated polyhydroxy terpenoid lactones (1-6), which all display the C22 17-ethyljatrophane carbon framework. Four of these (1-4) are delta lactones belonging to the previously described terracinolide type, and two of them (2, 3) are new. Two further new compounds have been named isoterracinolides A (5) and B (6) and exhibit an eight-membered lactone ring. Another isolated new compound is the jolkinolide-type, ent-abietane gamma lactone (7).


BACKGROUND: Participation in screening programs for malignant disease may have negative psychologic health effects that could outweigh the beneficial effects of the screening itself. The present study was designed to investigate the psychologic effect of attending a screening program for detection and removal of colorectal adenomas, which are precursors to colorectal cancer. METHOD: In 1983 a prospective. controlled screening study using flexible sigmoidoscopy to detect adenomas was started in T


We used a novel type of primer system, a system that uses stair primers, in which the primer sequences are based on consensus sequences in the DNA polymerase gene of herpesvirus to detect herpesviruses by PCR. A single PCR in a single tube detected the six major herpesviruses that infect the central nervous system: herpes simplex virus type 1 (HSV-1), and type 2 (HSV-2), cytomegalovirus (CMV), Epstein-Barr virus (EBV), varicella-zoster virus (VZV), and human herpesvirus 6 (HHV-6). We used the te


The three mammalian tachykinins, substance P (SP), neurokinin A (NKA) and neurokinin B (NKB), exert their physiological effects through specific receptors, NK1, NK2 and NK3, respectively. However, homologous binding studies have recently demonstrated that, contrary to the generally accepted belief, NKA could bind NK1 receptor with high affinity (Hastrup and Schwartz, 1996). Using COS-7 cells expressing the human NK1 receptor, we show that two simultaneous point mutations (E193L and V195R) in a r


BACKGROUND: Risk evaluation comparing the minimally invasive and standard aortic valve operations has not been studied. METHODS: Four surgeons were randomly assigned to perform the minimally invasive (L-shaped sternotomy) (group 1) or the conventional (group 2) operation in 120 patients exclusively. RESULTS: In both groups (n = 60) a CarboMedics prothesis was implanted in 90% of patients. There was no significant difference in the cross-clamping period (group 1, 60 minutes; range, 35 to 116 minu


In order to scale up medium-chain-length polyhydroxyalkanoate (mcl-PHA) production in recombinant microorganisms, we generated and investigated different recombinant bacteria containing a stable regulated expression system for phaC1, which encodes one of the mcl-PHA polymerases of Pseudomonas oleovorans. We used the mini-Tn5 system as a tool to construct Escherichia coli 193MC1 and P. oleovorans POMC1, which had stable antibiotic resistance and PHA production phenotypes when they were cultured i


OBJECTIVE: To examine the effect of alcohol abuse on the subsequent health status of elderly patients seen in an emergency department (ED). PATIENTS AND METHODS: A sample of 966 patients aged 65 or older who presented to one urban academic ED between the hours of 8 A.M. and 12 midnight was followed for 1 year. A personal interview was administered during the ED visit. Current problem drinkers had a score of 1 or greater on the CAGE questionnaire at ED presentation and drank within the prior 6 mo


A novel activator, N,N,Dimethylamino 4-benzyl laurate (DML) was used in the curing of acrylic bone cements, based on poly(methylmethacrylate). The water absorption characteristics were studied, and the behavior was found to be similar to commercial bone cements. The net amount of solubles were also in the same range as obtained for commercial cements cured with conventional activators. Biocompatibility tests were conducted on the neat activator (DML) and cured cements. The present study indicate


Strategies that prevent the attachment of N-linked carbohydrates to nascent glycoproteins often impair intracellular transport and secretion. In the present study, we describe a method to rescue the intracellular transport and secretion of glycoproteins mutagenized to delete N-linked glycosylation sites. Site-directed mutagenesis was used to delete N-linked glycosylation sites from a chimeric protein, TNFR-IgG1. Deletion of any of the three glycosylation sites in the TNFR portion of the molecule


The fibroblast intermediate conductance, calcium-activated potassium channel (FIK) is proposed here as a functional prototype for other IK channels which to date have undefined physiologic actions. FIK pharmacology in the 10T1/2-MRF4 myogenic fibroblast cell line was determined: to define the relationship of FIK to other IKs; to confirm a physiologic role for FIK; and, thus develop a hypothesis about IK channel family function. Whole cell patch-clamp electrophysiology was used to determine K(0.5


In the absence of K(+) on both sides of the membrane, delivery of standard activating pulses collapses the Shaker B K(+) conductance. Prolonged depolarizations restore the ability to conduct K(+). It has been proposed that the collapse of the conductance results from the dwelling of the channels in a stable closed (noninactivated) state (, J. Physiol. (Lond.). 499:3-15). Here it is shown that 1) Ba(2+) impedes the collapse of the K(+) conductance, protecting it from both sides of the membrane; 2


The aim of this study was to determine the effects of the exposure of pregnant females of a viviparous teleost Cnesterodon decemmaculatus to sublethal Cd solutions on the survival of their offspring. Ovigerous females were acclimated and accomodated in aquaria containing the following solutions: artificial freshwater medium (AFW) and AFW with 25, 50 and 100 microg Cd(2+) /L (as chloride). Part of the offspring born from control females was transferred to media contain-ing 25, 50 and 100 microg/L


OBJECTIVE: To explore the relationship between the partial middle turbinectomy and the prognosis. METHODS: Forty patients with nasal polyp and sinusitis were chosen. The partially middle turbinate was removed on one side and preserved on the opposite side. The time of postoperative debridement for nasal cavity, synechia of nasal cavity, and olfactory changes were assessed. RESULTS: The time of postoperative debridement of nasal cavity in patients with partial endonasal middle turbinectomy was sh


Measurements of residual perchloroethylene (PCE), a dry-cleaning solvent associated with human health effects, were made in dry-cleaned acetate cloth to enable improved characterizations of both occupational and environmental exposure. A limited sample size (25 acetate cloths) was used to explore the extent of inter-dry-cleaner variability in residual PCE and to characterize the effect of the pressing operation on residual PCE. A new method, which uses carbon-disulfide as the direct extracting a


The entire small bowel can be visualized on ultrasonography after ingestion of nonabsorbable, isosmotic polyethylene glycol electrolyte balanced oral solution, termed small intestine contrast ultrasonography. The aims of this study were to assess whether the ingestion of different volumes of sonographic contrast solution may differently affect (1) small bowel distention and thus its sonographic appearance and (2) the time to visualize the entire small intestine. An additional aim was to identify


Leptin is an adipocyte-secreted hormone that regulates body weight and exerts effects on hematopoiesis, reproduction, and immunity. The leptin receptor (OBR) shares sequence similarity and signaling capabilities with receptors for cytokines of the ciliary neurotrophic factor (CNTF) family. Our previous finding that CNTF and leptin exert similar anti-obesity effects and activate common neuronal signaling pathways, prompted us to investigate whether leptin may share with CNTF the ability to regula


Forty patients with Parkinson's disease (PD) were studied to explore the relationship between aspects of psychological functioning (depression, problems in motoric, cognitive and psychological areas) and physical illness (stage and duration of illness, functional disability, global cognitive status). Depression, motoric, psychological and cognitive problems were found to be significantly greater in patients suffering from severe as compared to moderate disease. Functional disability correlated m


Permatins are antifungal thaumatin-like proteins (TLPs) of the PR-5 family of pathogenesis-related proteins. They occur in many cereals, but little is known of their expression and roles. Permatin cDNA clones were produced and used to study expression in developing barley and oat seeds. Actin and CDC48 mRNAs declined rapidly following inoculation of barley spikes with Fusarium graminearum. Despite this, permatin mRNA levels remained constant or increased slightly. Studies of permatin gene expres


The posterior thoracic midline location is an unusual site for a congenital hemangiopericytoma. The authors report such a case that caused near fatal exsanguination of a newborn after vaginal delivery. Magnetic resonance imaging (MRI) studies of the mass were completed after hemostasis. These studies showed a well-defined border between the tumor and underlying trapezius muscle. The mass was removed successfully surgically and presumed initially to be a teratoma. Pathological diagnosis of the tu


The respiratory motoneuron is the critical link between the neural elements responsible for respiratory rhythm generation and the respiratory muscles. Studies of respiratory motoneurons provide important information on the mechanisms that govern respiratory motor output because of the obligatory synapse that exists between these respiratory motoneurons and the respiratory muscle fibers they innervate. This review focuses almost exclusively upon one type of respiratory motoneuron, the hypoglossal


The computer implementation of a peptide drug-design strategy has been developed. The system is named EmPLiCS (Empirical Peptide Ligand Construction System) according to the strategy of the system, which searches for peptide-ligand structures by referring to empirical rules that are derived from known protein 3D structures. The system was tested on several known peptide-protein complexes. The results demonstrated the ability of this system to detect key residues of peptides that are crucial for


Evoked magnetic responses to speech sounds [R. Näätänen, A. Lehtokoski, M. Lennes, M. Cheour, M. Huotilainen, A. Iivonen, M. Vainio, P. Alku, R.J. Ilmoniemi, A. Luuk, J. Allik, J. Sinkkonen and K. Alho, Language-specific phoneme representations revealed by electric and magnetic brain responses. Nature, 385 (1997) 432-434.] were recorded from 13 Japanese subjects (right-handed). Infrequently presented vowels ([o]) among repetitive vowels ([e]) elicited the magnetic counterpart of mismatch negativ


OBJECTIVE: This report describes the imaging characteristics of focal posttransplantation lymphoproliferative disorder. CONCLUSION: Posttransplantation lymphoproliferative disorder may be limited to the allograft. A focal complex mass in the renal allograft hilum surrounding the main renal blood vessels is a common finding and can be visualized with sonography. MR imaging can help increase diagnostic confidence.


BACKGROUND AND PURPOSE: To further describe the Superior Rectus Overaction/Contracture Syndrome (SRSy) first described by Jampolsky in 1964. METHODS OF STUDY: Twenty-five patients demonstrating findings characteristic of an isolated SRSy of various etiologies, but unrelated to superior oblique palsy or dissociated vertical deviation DVD, are reported. Eleven patients had surgery, consisting of superior rectus (SR) recession in ten patients and a Knapp procedure to the fellow eye (with presumed d


OBJECTIVE: To determine whether slowing of gastric emptying and glucose absorption with guar gum would reduce the fall in blood pressure after an oral glucose load in older subjects. DESIGN: A randomized, experimental, cross-over study. SETTING: Department of Nuclear Medicine, Royal Adelaide Hospital, Adelaide, SA, Australia. PARTICIPANTS: Ten healthy subjects, age 67 to 78. MEASUREMENTS: Simultaneous measurements of gastric emptying, blood pressure, blood glucose, serum insulin, and oral glucos


There is a series of different therapies for the treatment of anogenital warts. Home therapy should be reserved only for initial therapy of simple cases. Such approaches include podophyllotoxin solution, podophyllotoxin cream,imiquimod cream and adjuvant interferon gel. Recurrent disease and disseminated disease must be treated by the physician. Choice of therapy depends on the morphology, the extent of the disease and also on the immunological status of the patient. Therapy should be discussed


Toxicity data collected in a laboratory setting are the primary source of potency information used for regulatory, modeling, or risk assessment purposes. However, the relative reproducibility of such toxicity data is rarely discussed. This study investigated the reproducibility of growth impairment data for the freshwater ciliate Tetrahymena pyriformis exposed to a structurally diverse group of chemicals of varying hydrophobicity within different modes of toxic action, either non-covalent narcos


While studying the cellular localization and activity of enzymes involved in heparan sulfate biosynthesis, we discovered that the published sequence for the glucuronic acid C5-epimerase responsible for the interconversion of d-glucuronic acid and l-iduronic acid residues encodes a truncated protein. Genome analysis and 5'-rapid amplification of cDNA ends was used to clone the full-length cDNA from a mouse mastocytoma cell line. The extended cDNA encodes for an additional 174 amino acids at the a


The mechanism of production of xylitol from xylose by Candida guilliermondii was studied using chemostat cultures and enzymatic assays. The maximum dilution rate in aerobic conditions was 0.34 1/h. No xylitol was produced. Under oxygen-limited conditions xylose uptake was impaired and glycerol accumulated but no xylitol was detected. Under transient oxygen limitation, caused by a gradual decrease in the agitation rate, onset of xylitol, acetate and residual xylose accumulation occurred simultane


PURPOSE: To compare the vasoactive properties of the docosanoid unoprostone, its free acid, and different members of the prostanoid family on isolated perfused pig retinal arterioles to assess their potential to modulate retinal blood flow. METHODS: Segments of porcine retinal arterioles were dissected, cannulated, and perfused, and their diameter monitored during either intraluminal or extraluminal application of increasing doses (10(-10)-10(-4) M) of either the docosanoid unoprostone isopropyl


This study investigated repetition priming in the recognition of famous voices, recording reaction times (RTs) and event-related brain potentials (ERPs). In Experiment 1, a facilitation was found in RTs to famous but not to unfamiliar voices when these had been primed by a different voice sample of the same speaker earlier in the experiment. However, ERPs to both famous and unfamiliar voices showed repetition priming in terms of an increased P2 component, which is thought to be generated in the


High-resolution structures of the ligand binding core of GluR0, a glutamate receptor ion channel from Synechocystis PCC 6803, have been solved by X-ray diffraction. The GluR0 structures reveal homology with bacterial periplasmic binding proteins and the rat GluR2 AMPA subtype neurotransmitter receptor. The ligand binding site is formed by a cleft between two globular alpha/beta domains. L-Glutamate binds in an extended conformation, similar to that observed for glutamine binding protein (GlnBP).


BACKGROUND: Perioperative myocardial infarction may not be diagnosed correctly because World Health Organization criteria are often not met and creatinine kinase myocardial fraction (creatinine kinase/creatinine kinase MB isoenzyme; CK/CK-MB) ratios can be difficult to interpret. Cardiac troponin (cTn) I and cTnT are the most sensitive and specific markers of myocardial cell necrosis currently available but are not widely used in surgical practice. The aim was to compare cTnI and CK/CK-MB ratios


A respiratory syncytial virus (RSV) vaccine will need to be administered by 1 mo of age to protect young infants; therefore, it will need to be effective in the presence of maternally acquired RSV Abs. In the present study, the immunogenicity and efficacy of two live attenuated RSV vaccine candidates of different level of attenuation were evaluated in mice passively immunized with varying quantities of RSV Abs. The replication of the RSV vaccines was suppressed in the lower, but not the upper, r


A total of 1604 samples of crystalline bedrock groundwaters in Norway have been analysed for pH, major and minor elements. A subset of 476 samples were also analysed for a wide range of trace elements by ICP-MS. The pH of the samples ranges from 5.4 to 9.8, with a predominance of pH values between 8.0 and 8.2. The data-set is divided into five 20-percentile groups according to increasing pH. The concentrations of 60 elements are then displayed as box-plots for each pH group. A line through the f


An analysis system of informational uncertainties for accidental risk assessment of chemicals is introduced. Statistical test methods and fuzzy sets method can do the quantitative analysis of the input parameters. The uncertainties of the model can be used by quantitative compared method for the leakage accidents of chemicals. The estimation of the leaking time is important for discussing accidental source term. The uncertain analyses of the release accident for pipeline gas (CO) liquid chlorine


OBJECTIVES: The goal of this study was to determine stimulant effects on story grammar, comprehension, and errors in the narratives of children with attention deficit hyperactivity disorder (ADHD) with and without comorbid language impairment. Previous research has demonstrated impairments in the narrative abilities of children with ADHD, but the effect of the primary treatment modality (methylphenidate) is unknown. METHODS: Fifty children with ADHD (7 to 12 years of age) were stratified for lan


Superantigens (SAgs) are proteins produced by pathogenic microbes to elicit potent, antigen-independent T cell responses that are believed to enhance the microbes' pathogenicity. Here we show that the human lymphotropic herpesvirus Epstein-Barr virus (EBV) transcriptionally activates the env gene of an endogenous retrovirus, HERV-K18, that possesses SAg activity. SAg activity was demonstrated by MHC class II dependent preferential activation of TCRVB13 T cells in response to murine B cells trans


Initially identified in high-grade gliomas, mutations in the PTEN tumor-suppressor are also found in many sporadic cancers and a few related autosomal dominant hamartoma syndromes. PTEN is a 3'-specific phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3) phosphatase and functions as a negative regulator of PI3K signaling. We generated a tissue-specific deletion of the mouse homolog Pten to address its role in brain function. Mice homozygous for this deletion (PtenloxP/loxP;Gfap-cre), develope


Helium is considered to be the ideal carrier gas for gas chromatography/mass spectrometry (GC/MS) in general, and for use with an ion trap in particular. Helium is an inert gas, can be used without special precautions for security and, moreover, it is needed as a damping gas in the trap. A disadvantage of helium is the high viscosity resulting in long GC run times. In this work hydrogen was tested as an alternative carrier gas for GC in performing GC/MS analyses. A hydrogen generator was used as


Mucins have been ascribed both pro- and anti-adhesive functions. To clarify how both functions can be embodied in the same molecule we studied the interaction of human ocular mucins with mica and with mucins deposited on mica. Adhesion energy and forces of interaction were evaluated as a function of speed of approach, dwell time at maximum extension, and presence of divalent cations in the imaging buffer. Mucins were tethered to an AFM gold-coated tip. Repeated cycles of approach and retract to


The authors describe spontaneous tears of the retinal pigmented epithelium in three eyes of two patients with age-related macular degeneration. In all instances the development of the tear was associated with an occult choroidal neovascular membrane detected by fluoroangiography. The authors describe typical clinical features and contemporary views regarding the pathogenesis of the tear.


Celiac disease (CD) is a multifactorial disorder of the small intestine caused by a permanent dietary intolerance to gluten. The combined presence of the HLA class II DQA1*0501 and DQB1*0201 alleles represents the major genetic component for disease predisposition. It has been shown that the Saharawi refugees living in northern Africa have a very high frequency of CD. In the present study we analysed this population to evaluate the degree of association with CD of the haplotypes and genotypes at


Mastocytosis is defined by a pathological increase in mast cell numbers in tissues. Recent clinical observations on rare manifestations highlight the diversity of this disease. The diagnosis is now aided by new surrogate markers. At the molecular level, recent studies have reinforced the role of activating mutations in KIT in the etiology of mastocytosis. These findings provide a conceptual basis for the development for new therapeutic strategies.


Bloom provides a masterful synthesis of recent advances in word-learning, placing them within the framework of abiding theoretical issues. I will augment and challenge his approach by underscoring the significance of word extension for questions concerning (a) the origin and evolution of infants' expectations, and (b) domain-specificity in word-learning.


PURPOSE: To present the unusual case of chronic granulomatous disease (CGD) manifestating as pulmonal aspergillosis in female enzygotic twins during the neonatal period. To demonstrate and to discuss the complex diagnosis and the diagnostic value of FDG-PET in monitoring the spread and activity of the disease. PATIENTS: Plain chest x-ray and CT of the lung showed multiple, mostly round lesions in one of the former preterm twins at the age of 8 weeks. The girl with known ASD II was then suffering


The 20S proteasome is the catalytic portion of the 26S proteasome. Constitutively expressed mammalian 20S proteasomes have three active subunits, beta 1, beta 2, and beta 5, which are replaced in the immunoproteasome by interferon-gamma-inducible subunits beta 1i, beta 2i, and beta 5i, respectively. Here we determined the crystal structure of the bovine 20S proteasome at 2.75 A resolution. The structures of alpha 2, beta 1, beta 5, beta 6, and beta 7 subunits of the bovine enzyme were different


Edición digital del libro en papel impreso en el año 2000.
Se analiza el cambio de siglo en Santander como un cambio social, de ciclo económico, de orientación productiva, de transformación urbana, en contraste con el mantenimiento de las pautas tradicionales de comportamiento político.


Ausgangslage: Die Haaranalyse gilt, ebenso wie die gängigere Urinuntersuchung auf psychotrope Substanzen, als leicht durchführbar und interpretierbar. Zwischen beiden Matrices gibt es jedoch grundlegend verschiedene biologische, präanalytische und analytische Aspekte, um zu einem aussagekräftigen Befund zu gelangen.

 Einflussgrößen: Urin und Haar sind hoch variabel zusammengesetzt. Morphologische Schäd


Background: Although ileoanal anastomosis has become popular for ulcerative colitis, in an emergency situation patients must undergo ileostomy. Aim: A novel ileocecal valve-preserving ileostomy procedure was devised to reduce high output liquid loss. Method: After total colectomy, the ascending colon was clamped and the terminal ileum and ileocecal valve were isolated from the cecum by dissection. The ileum was then brought out through a c


We have mapped 11 novel, anonymous genetic markers to rat chromosome 2. The rat ceruloplasmin gene (Cp) had been previously mapped to chromosomes 2 and 7q11→q13 by two different methods. To resolve the assignment and to localize the Cp gene on the rat genetic linkage map, we used linkage analysis to confirm that rat Cp lies on chromosome 2.


(A) Cultures were inoculated with equivalent numbers of ES cells bearing different Atrx alleles as indicated, and were serially passaged. After the indicated days of coculture, DNA extracted from a sample of cells was analysed by Southern blot to detect the Atrx alleles. DNA was digested with SpeI, and the membrane was hybridised with the 20/27 probe shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.00


Abstract

This research intends to find the answers of two problems. First, the factors that led to the unimplemented jurisdiction of the General Court of the Indonesian Armed Forces (TNI) that perform general crime as mandated by Decree No. VII / MPR / 2000 and Act No. 34 of 2004. Second, the perception of the military conception of justice with jurisdiction over soldiers who committed the crime. This study is a nor


<sec><st>Introduction and objectives</st> New methods of high throughput sequencing provide unparalleled access to the human genome and transcriptome. We hypothesised that next generation DNA sequencing technologies would allow us to identify an elusive novel disease gene for a pulmonary vascular disease inherited as an autosomal dominant trait: The <it>HHT3 </it>interval on chromosome 5 is predicted by linkage studies to contain a mutation causing pulmonary arterio


We present evidence of bar-induced secular evolution in galactic discs using 3.6 μm images of nearby galaxies from the <it>Spitzer</it> Survey of Stellar Structure in Galaxies (S4G). We find that among massive galaxies (<it>M</it><inf>*</inf>/M<inf>&odot;</inf> > 1010), longer bars tend to reside in inner discs having a flatter radial profile. Such galaxies show a light deficit in the disc surrounding the bar, within the bar radius and often


Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to provide a scientific opinion on health claims pursuant to Article 13 of Regulation (EC) No 1924/2006 in the framework of further assessment related to a combination of Lactobacillus helveticus CNCM I-1722 and Bifidobacterium longum subsp. longum CNCM I-3470 and alleviation of psych


Objetivo: determinar la frecuencia de asistencia a la consulta odontológica durante el embarazo de las mujeres que asisten al primer control neonatal y que tuvieron su parto en la misma institución, y explorar asociaciones entre hábitos de higiene oral, hábitos de salud, conocimientos y creencias, y la asistencia a la consulta odontológica.

Materiales y métodos: estudio de corte transversal descriptivo. Se inclu


&
SNPs used in this study were originally studied in Almeida et al [14]. †Distance of the SNP is relative to the SCA10 expansion and is expressed in base pairs. Locations upstream and downstream of the SCA10 expansion are denoted by negative and positive values, respectively. *, The common disease haplotype of Mexican and Brazilian families in our SCA10


Background: Dialysis in older adults with chronic kidney disease (CKD) and comorbidities may not be associated with improved life expectancy compared to conservative management. To inform clinical practice, we performed a systematic review of all available studies examining this hypothesis. Methods: We performed a systematic review of retrospective and prospective cohort studies of older adults with stage-5 CKD who chose


<sec><st>Background/aim</st> Evidence of the impact of green spaces on pregnancy outcomes is still limited. We analysed green spaces and socio-economic position (SEP) as effect modifiers of the effect of high temperatures and air pollutants (PM10, NO2 and O3) on the risk of preterm birth (PB). </sec> <sec><st>Methods</st> A cohort of new-borns in Rome, from April to October, 2001–2013, were analysed. Pre-terms, identified through the Certificate of Deliv


Background: Bipolar disorder (BD) has been associated with temperamental and personality traits, although the relationship is still to be fully elucidated. Several studies investigated the genetic basis of temperament and character, identifying catechol-O-methyltransferase (COMT), brain derived neurotrophic factor (BDNF), and serotonin transporter (5-HTT) gene variants as strong candidates. Methods: In the GECO-BIP study


ABSTRACT
Tubal ligation is a very effective contraception method, but only a few women interested to do it. There are several factors influencing tubal ligation choice including husband’s support, motivation, information access, and service quality. The purpose of this research was to identify the factors that influence the low interest on using tubal ligation contraception method. This was a cross sectional study with purposive sampling technique


Background: Caucasians with red hair and fair skin have a remarkably increased risk of malignant melanoma compared to non-redhead Caucasians. Objectives: With the aim of a reliable melanoma diagnosis in redheads, the application of dermatofluoroscopy was analyzed in 16 patients with red hair. Most of them had been included in a clinical dermatofluoroscopy study for patients with the suspicion of melanoma. We examined whe


Introduction: There are a growing number of patients undergoing bariatric surgery requiring lifelong follow-up. Therefore, follow-up care can no longer be covered by specialized outpatient clinics alone due to the sharp rise in the number of bariatric patients. Bariatric Patients in Primary Care: Postoperative Nutrition and Lifestyle Management (BagEL) is a survey to evaluate a newly developed structured disease management program including nutrition and lif


Using carbon dioxide (CO2) as a feedstock to synthesize various polymers has drawn much attention. One-pot multicomponent tandem polymerization (MCTP) with great synthetic simplicity and efficiency is a powerful strategy for the synthesis of new CO2-based luminescent polymers. In this work, we successfully developed a new one-pot MCTP combining three sequential carboxylation-cyclization-esterification reactions of CO2, diynes a


In the static and dynamic workflow of carbonate reservoirs, stratigraphic correlation of well
data is one of the first and most influent steps. Indeed, facies distribution and petrophysical
properties mainly control flow simulation and are often computed thanks to geostatistical
methods, on grids based on stratigraphic correlation and structural data interpreted from
seismic data (Borgomano [2008]).


Abstract
Purpose: To gain insight into genomic and trancriptomic subtypes of ductal carcinomas in situ of the breast (DCIS).

Experimental Design: We did a combined phenotypic and genomic analysis of a series of 57 DCIS integrated with gene expression profile analysis for 26 of the 57 cases.

Results: Thirty-two DCIS exhibited a luminal phenotype; 21 were ERBB2 positiv


AbstractPurpose:
Consensus molecular subtyping (CMS) of colorectal cancer has potential to reshape the colorectal cancer landscape. We developed and validated an assay that is applicable on formalin-fixed, paraffin-embedded (FFPE) samples of colorectal cancer and implemented the assay in a Clinical Laboratory Improvement Amendments (CLIA)-certified laboratory.
Experimental Design:
We performed an in silico experiment to build an optimal CMS


A rear load mounted on a motorcycle has significant influences on the oscillatory behaviors of the wobble mode and the weave mode. In this paper, two kinds of rear load suspension assemblies are proposed and investigated for the purpose of stabilizing the wobble and weave oscillations. Effectiveness of the rear load suspension assemblies on the wobble and


Introduction: In 1994, the city of Cincinnati, Ohio was named the most livable city in America by Places Rated Almanac (Clark, 1993). Couched within this distinction is the variance of perceived ca


In\nthis paper, mechanoresponsive fluorescent molecules with high\n“off–on” contrast ratios that undergo molecular\npacking transformation from amorphous phase to crystalline by scratching\nare first reported. Three 2-aminobenzophenone derivatives 1, 2, and 3, which possess crystallization-induced\nfluorescence characteristics, exhibited bright cyan, blue, and green\nfluorescence colors with “off–on” contrast ratios\nas high as 175-fold by


As\nan essential modification, O-linked β-N-acetylglucosamine\n(O-GlcNAc) modulates the functions\nof many proteins. However, site-specific characterization of O-GlcNAcylated\nproteins remains challenging. Herein, an innovative material grafted\nwith nitro-oxide (N→O) groups was designed for high affinity\nenrichment for O-GlcNAc peptides from native proteins.\nBy testing with synthetic O-GlcNAc peptides and standard\npr


The mechanistic impact of water addition to SmI2 on the ground state and rate-limiting transition\nstate structures in the reduction of benzyl bromide was determined using UV−vis spectroscopy, cyclic\nvoltammetry, vapor pressure osmommetry, and stopped-flow spectrophotometric studies. The results\nobtained from these studies show that, upon addition of water, SmI2 in THF (or DME) becomes partially\nwater-solvated by displacing metal-coordinated solve


The discovery of the potent and selective PDE-5 inhibitory activity of a pyrroloquinolone scaffold\nprompted us to explore the SAR of its acyl derivatives. During the course of these studies,\nthree structural series were found with Ki values for PDE-5 in the subnanomolar range.\nSystematic modification of one of these leads produced a compound with excellent selectivity\nfor PDE-5 over other phosphodiesterases and oral bioavailability of 15% in male rat


(A) Metabolic map in the same style as S2 Fig for aceF mutant strain vs. PAO1 reference in SCFM2 in absence (left) and presence (right) of acetate. (B) Parallel plot showing the number of differentially expressed proteins, separated by metabolic COG categories, in aceE (cyan) and aceF (magenta) mutan


Table of top COVID19-associated full amino-acid IgH clonotype (CDR3+VJ), containing their CDR3 amino acid sequence, V and J gene choices, Ppost and value, posterior probability of being associated (p), and index of reported antibody match from the curated database (index column in S3 Table).<


Top Left: One frame of the DeepLabCut output, with decoded positions of left and right paws in blue and red. Top right: Relative decoding accuracy when the decoder was restricted to use signals from just one brain region, as a fraction of the R2 using all signals from all brain regions. Area acronyms are provided in Table 2


The figure shows a z-score transformed PAC, PAC-Z. (a) The PAC-Z for the phrases and the sentences at each hemisphere. Each figure was created by averaging 8 sensors which showed the biggest PAC-Z over the ROI. A z-score transformation with Bonferroni correction was conducted to test the significance, which lead to the threshold to be 3.73 corresponding to p-value equals 0.05. (b) The figure shows how sensors were selected at each


Each ommatidium of the compound eye contains eight photoreceptors, or retinula cells, R1-8. R1-6 are a homogenous group, found in every ommatidium of the eye. Over the majority of the eye, R7 and R8 occur in two subtypes, R7y and R8y in about 70% of ommatidia, and R7p and R8p in the remaining 30% [17]. Most fly photoreceptors have complex sensitivity funct


The bounds for the sum of distinctions integrated information ∑φd and the sum of relations integrated information ∑φr.


1
T90, the temperature at which HIV-1 infectivity decreases by 90% in 1 h, and the standard error of the mean (SEM) of at least three independent experiments.
2
HIV-1 PSVs, produced by transfection of 293T cells using env expression plasmids pSVIIIexe7 (JR-CSF, JR-FL, ADA), pCAGGS (SF162, SIVmac239), pcDNA3.1 (LAI), or pVSV-G (VSV-G) in combination with the backbone vectors pSG3Δenv (clade B) or Q23Δenv (clade A).
3<p


Top, Representative immunoblots of each protein and GAPDH, as a loading control. Bottom, Bar graphs showing the densitometric analysis of Western blots. For each treatment series, protein values were normalized to GAPDH and expressed as a ratio relative to control K562 cells. Data are presented as mean ± SD from at least 3 separate experiments. *, P<0.05 vs control. Dash-dotted line represents transcription levels of genes encoding HSP70, Sti1 and hnRNP L, as deter


(A, B, C, D) Lung histology (H&E staining, 200x magnification) of mice exposed to hyperoxia for 60 h, shown at 0, 1, 3, and 7 days, respectively, after treatment with PBS. (E, F, G, H) The same groups as above shown at 400x magnification. (I, J, K, L) Histolo


Filled points identify sample sites, color-coded to illustrate the seven genetic populations revealed by the analysis with Structure (see Fig 5). The principal ecological divisions of the St. Lawrence River and estuary are indicated with broken, vertical lines. Pale triangles identify principal towns and cities mentioned in the text. See <a href="http://www


Summary of results for testing of B. cereus BcFL2013 compared to B. cereus G9241 and B. anthracis Ames.


A: Count of visASCs that migrated after 24 hours of culture toward the lower surface of the migration chamber, the compartment containing culture medium supplemented with 100 ng/mL of SDF1α or without SDF1α. Bar = 100 μm. B: The migration rate was calculated by dividing the number of cells detected in the presence of SDF1α by those observed in the absence of SDF1α. The results are presented as mean migration rate values ± standard error (NonMS, n =


A) Pituitary neoplasm, B) Intestinal adenocarcinoma, C) Hematopoietic neoplasia, and D) Kidney: Benign renal cysts. H&E stain; 200X.


In Alberto Savinio’s Tutta la vita, and particularly in the stories Il suo nome, Paradiso terrestre, La pianessa and Eònio, the proper name is an emblem of the intersection between reality and myth in the literary creative activity, and it is connected with the cultural and politic thought explained by the author himself in Fine dei modelli. This is a very import


Lanthanide(III)\nions (Ln3+) in coordination compounds\nexhibit unique luminescence properties with narrow and characteristic\nf-f transitions throughout the visible and near-infrared (NIR) ranges.\nIn addition, some Ln3+ such as Pr3+, Sm3+, Dy3+, Ho3+, Er3+, and\nTm3+ possess an exceptional ability, although less


The\n4H+/4e– reduction of O2 to\nwater, a key fuel-cell reaction also carried out in biology by\noxidase enzymes, includes the critical O–O bond reductive cleavage\nstep. Mechanistic investigations on active-site model compounds, which\nare synthesized by rational design to incorporate systematic variations,\ncan focus on and resolve answers to fundamental questions, including\nprotonation and/or H-bonding aspects, which accomp


Synthesis of the 2,9-dimethyl-4,7-diaza-4-alkyl-2,9-decanedithiol (1, alkyl = morpholinylethyl in a, and alkyl =\npyrrolidinylethyl in b), following a widely used synthetic scheme for diaminedithiol (DADT) ligands, led to the isolation\nof 1-alkyl-2-(1‘-methyl-1‘-sulfanylethyl)-3-(2‘ ‘-methyl-2‘ ‘-sulfanylpropyl)diazolidine (3) as the major product. Both ligands\n1 and 2 gave complex


Transition metal-doped ferrites are\nattractive candidates for a\nwide range of applications including catalysis and electronic and\nmagnetic devices. Although their bulk characteristics are well-understood,\nvery little is known about their surface properties at the molecular\nlevel. Here, we demonstrate high reactivity of NiFe2O4 (111) surfaces, a Ni-doped ferrite, by elucidating the surface\nstructure and water adsorption mechanism using density f


Fusion proteins are expressed under the control of the 35S promoter. (A) Interaction of YFPN-SPI and YFPC-DCP1 (left) in transiently transformed N. benthamiana leaves 72 h post-transfection at DCP2-mCHERRY labeled P-bodies (middle). Right pictures show the overlay of the left (green) and middle (magenta) pictures. (B) Interaction of YFPN-SPI and YFPC-DCP1 in


(A) Additive effects of ΔrecQ and lexA3(Ind−) mutations. ΔrecQ lexA3(Ind−) (SMR10683, ) cells show significantly greater TLD resistance than either ΔrecQ (SMR10436, ▴) at t≥120 min or lexA3(Ind−) (SMR10669, ▪) at t≥270 min. Parental strain AB2497 (♦). (B) Additive effects of ΔrecQ and sulA</i&gt


In figure 2, the continuous blue line is for low hyperactivity-impulsivity, and the dashed red line is for high hyperactivity-impulsivity.


qRT-PCR analysis of miR-361-5p (A) and VEGFA (B) expression in A431, HaCaT, primary human dermal lymphatic (LEC) and blood endothelial cells (BEC). Fold differences ± S.D. in expression levels with regards to the references (RNU6B and ACTB, for miRNA-361-5p and miRNA-361-5p, respectively) are plotted. Data represent at least three independent experiments performed in duplicate. Two-tailed, unpaired t-tests were used to calculate P values (one, two and three asterisks


A. Biofilms of TG agn43-lacZ from an ON or an OFF colony in microfermentors. The biofilms were grown for 24 and 48 h; the biomass growing on the spatula was resuspended in 10 mL M63B1Gluc and the optical density at 600 nm was measured. (B): ON colony, (W): OFF colony; B. Pictures of microfermentors after 48 h growth. ***: p<0.0001. C. Immunodetection of Ag43 in TG agn43-lacZ&lt


Histochemical GUS assay of transgenic plants containing a LORE1a LTR::GUS fusion. (A) GUS staining was observed at the tip of keel containing released pollen grains in a transgenic L. japonicus plant (upper), while no GUS activity was detected in the control Gifu (lower). (B,D) Close up of pollen grains after GUS staining of transgenic (B) and control Gifu (D) samples. Scale bars correspond to 50 µm. (C,E) Hoechst 33258 staining of the pollen grains


Simulation scenarios consisted of combining estimated parameter distributions from (a) Patuxent National Research Refuge, Maryland, USA and (b) Rock Creek Park, Washington, DC, USA with different numbers of vernal pools (n): 5, 10, 25, 50, 75, and 100 pools. One thousand iterations were run for each scenario. Quasi-extinction occurred when no pools produced metamorphic wood frogs for two consecutive years. Quasi-extinction was not observed in simulations involving &gt


(A) Conserved targeting of 3′-UTRs of the human int β1 by miR-29c (underlined). The wild-type and mutant sequences of int β1 3′-UTR are listed for comparison. (B) Dual-luciferase reporter assay for target gene int β1 in 95D cells transfected with miR-29c mimics. **p<0.01 (Student's t-test, n = 3 ). (C) Dual-luciferase reporter assay for target gene int β1 in 95C cells with or without transfecting mir-29c inhibitors. **p<0.01


For a more interactive version see S1 File.

(PDF)


+
The p-value indicates the probability of a significant relationship between the percentage of each particle size (in mm) for each growth medium at the start of the experiment and the CGW:P ratio. F test is significant at n.s.p>0.05; ***p<0.001; **p<0.01; *p<0.05. n.s.: No


(A)—outline of the infusion protocol; (B)–effect of intralipid and heparin infusion (n = 3 per group); (C)–effect of nicotinic acid infusion (n = 3 per group), (D)–plasma [U-13C]palmitate enrichment (n = 10 per group); C—control with phosphate buffered saline (PBS) infusion (grey circles); I+H—intralipid and heparin infusion (closed circles); NA—nicotinic acid infusion (grey circles). Values represent mean +/- SD.


Data from 72-hour timelapse imaging of Plk4-inducible MCF10A cells stably expressing histone H2B-mNeonGreen and mScarlet-tubulin treated with 1 nM paclitaxel or 1 nM vinorelbine +/− 2 μg/mL dox to induce Plk4 expression. (A, B) In cells treated with these low concentrations of microtubule poisons that did not substantially induce multipolar spindles on their own, dox-inducible Plk4 expression increased the incidence of multipolarity prior to (<b&


Colours refer to the cost category; the grey set of boxes are collectively referred to as the “care costs”. The components of each cost are given in Table 2 where colour indicates to which category they belong. Investment costs refer to the categorisation of costs used in the return on investment analysis, see below.


a Age group based on mean or median age. Only 35 of the 50 studies included data on age. The remaining studies reported no information on age specific to the migrant participants or included a range that overlapped more than one age group.

b Studies where all migrants were ≤ 18 years were considered to be studies of children.

c According to World Bank classification
<p&gt


Maximum J-factors for each of the five sequences, with (closed circles) and without (open circles) promoter, are plotted with respect to each sequence's G+C content. For nucleosomes, G+C content strongly correlates with nucleosome occupancy [17]. In contrast, it appears that G+C content and loopability are anticorrelated. Loop lengths plotted here are the sa


HeLa M (A) and BeWo (B) cells were incubated with liposomes in triplicate for 24 and 48 hours followed by incubation with MTT for 3 hours. Cell viability is shown as the percentage of the total MTT signal detected in untreated control cells. Error bars corresponds to the standard deviation (n = 4). Significant changes in cell viability (* indicates p < 0.05) compared to protein-free liposomes (see dashed line for 48 hour time point) was determine


(A, B): Bright field images of normal and microgrooved tissue culture flasks, respectively. (C, D): Bright field images of control and microgrooved surfaces, respectively. (E, F): SEM images of control/normal and microgrooved surfaces, respectively. (G): Quantitation of microgrooved/modified surface area (cm2) against the total cell growing surface area. Scale bars = 50 μm (C, D), 20 μm (E, F). Results shown are mean ± SEM. ***, P ≤ 0.001.


Ratings of 12 pictures of an internet search by 8 to 14-year-old children in school, class by class. Self-assessment manikins were used for valence and arousal ratings (SAM; [41]. Pictures were presented for 6 s each in different picture sets on a screen in front of the class.

(DOCX)


Data are means ± SEM (n = 15). Values marked with the same letter are not significantly different for the same leaf number in different treatments (Tukey’s HSD at P < 0.05).


Presented\nherein is a novel synthesis of pharmaceutically privileged\nspiroindoline derivatives via cascade reactions of N-methyl-N-nitrosoanilines with diazo homophthalimides.\nA group of mechanistic studies disclosed that the formation of product\ninvolves an unusual reaction mode of N-methyl-N-nitrosoaniline featuring an initial C(sp2)–H\nbond activation/alkylation followed by a C(sp


Mixed\nlead–tin hybrid perovskite alloy CH3NH3(Pb1–x\nSnx)I3 attracted significant attention lately because\nof the reduction of its band gap below both end compounds, which makes\nit a promising bottom cell material in all-perovskite tandem solar\ncells. The effect is a consequence of a strongly nonlinear dependence\nof the alloy band gap on c


Three new 21-membered macrocyclic\nbenzenoid ansamycins, trienomycins\nJ–L (1–3), together with seven\nknown analogues, trienomycins A–G (4–10), were isolated from liquid culture of the moss soil-derived\nactinomycete Streptomyces cacaoi subsp. asoensis H2S5. The structures of the new compounds were elucidated by extensive\nNMR spectroscopic analysis and HRESIMS data. The absolute co


The synthesis and characterization for trifluoromethanesulfonate (triflate) salts of the first definitive examples of\ncyclotriphosphinophosphonium and cyclodiphosphinophosphonium cations are described, representing new prototypical\nframeworks in the rational and systematic development of catena-phosphorus chemistry. Addition of methyl triflate\n(MeOTf) or triflic acid (HOTf) to cyclotetraphosphines (tBuP)4 (1a)


This study investigated the cotransport\nof titanium dioxide nanoparticles\n(nTiO2) and fullerene nanoparticles (nC60), two of the most widely utilized nanoparticles,\nin saturated quartz sand under a series of ionic strengths in NaCl\nsolutions (0.1–10 mM) at both pH 5 and 7. Under all examined\nionic strengths at pH 5, both breakthrough curves and retained profiles\nof nTiO2</su


A recent ion mobility-mass spectrometry (IM-MS) study\nof the nonapeptide\nbradykinin (BK, amino acid sequence Arg1-Pro2-Pro3-Gly4-Phe5-Ser6-Pro7-Phe8-Arg9) found evidence for 10 populations\nof conformations that depend upon the solution composition [J. Am. Chem. Soc. <


α Student test

β Chi2 test

δ Fisher’s exact test

Patients’ characteristics.


The phylogenetic tree is based on the mapping of Illumina data for representative strains of the complex to the newly generated M. africanum GM041182. The phylogeny was reconstructed using Neighbor-joining and is based on 9,699 variable positions in at least one strain. One thousand bootstrap pseudo-replicates were used to assess clade reliability. All nodes had more than 90% support. The positions of RD900h and RD900h are indicated. Numbers in branches refers to the


(A) Representatives and summary of DNA content of cells detected by flow cytometry. The results are expressed as mean±SD of three independent experiments. *, P<0.05, **, P<0.01. (B) Representatives and summary of DNA content in CACNA2D3 silenced cells. Scramble siRNA was used as a control. **, P<0.01. (C, D, E) Expression of proteins was detected in CACNA2D3 overexpressed cells (C, E) and silenced cells (D). GAPDH and tub


a) Scores plot between the selected principal components. The explained variances are shown in brackets. b) Scree plot shows the variance explained by principal components. The green line on top shows the accumulated variance explained; the blue line underneath shows the variance explained by individual principal components.

(TIF)


(A-C) Representative immunoblot bands for TLR4, GFAP and Iba-1. GAPDH served as an internal standard. Relative protein levels were calculated as fold increases vs. the sham group. Data are expressed as the mean ± SD. *P < 0.05 vs. the sham group; *# P < 0.05 vs. the IR group. (D) Blocking CXCL12/CXCR4 expression in glial cells with co-localized TLR4 expression. Representative


Phylogenetic tree obtained with the AOX proteins of A. thaliana, C. sinensis and C. clementina.


(A) Patients were dichotomized by KRAS mutation status and miR-345. (B) Patients were dichotomized by BRAF wt mutations status and miR-345. (C) Patients were dichotomized by PI3KCA mutation status and miR-345. (D) Patients were dichotomized by double wt mutation status and miR-345. The P-value refers to the Wald test.


There are two peaks (at 50,000 tweets, Alpha = 0.394, and at 160,000 tweets, Alpha = 0.391), and a large drop in between, due to a topic shift.


The specific miRNA targets were predicted using Targetscan 4.2. The gene targets for those miRNAs highly expressed in the airway (2-(ΔΔCT)>10). Targets that shared a high frequency across the miRNAs analysed (28 miRNAs in the highly expressed group) are plotted. Likewise, the transcription factor binding sites within 5 kb of the miRNA start site, for the same set of miRNAs, were analysed using UCSC Genome Browser. Abbreviations; MAPK, mitogen activated protein


A: “CD” reads; B: “Not IBD” reads; C: overall attention. In each phenotype, nucleotide frequencies are scaled by the overall entropy for all Blautia testing reads and plotted as sequence logo and averaged attention weights are represented by the color map (brighter colors represent larger attention weights). Two areas have attention that differentiate Blautia in Crohn’s Disease from Blautia in Non-IBD, while a third site is import


To explore whether there are particular patterns of anti-peptide reactions among all serologically positive T. gondii cat sera, a XY-fused Selforganizing Kohonen Network analysis (XYF-SKN) was performed. Figure (A) presents the clustering of various groups of T. gondii positive cat sera in a Y-map. Most of the sera derived from cats infected with non-canonical T. gondii types (A), infected by clonal types I, II or III (I, II, or I


Köppen-Geiger climate classification [45] letter: B: arid, s: summer dry, k: cold arid.


Comparison of meta-analysis results from this study to results in the published literature1: meta-relative risk (RR; random effects model), 95 percent confidence interval (CI) and number of studies (N).


The fetal body weights (BW) in uninjected animals were 4.37±0.10 grams whereas those injected with PBS were 4.55±0.11 grams. Fetal lung weights (LW) in the uninjected and PBS treatment were 131.23±7.05 mg and 136.69±4.36 mg, respectively. The LW/BW (expressed as a percent) in PBS-injected fetuses was 3.02±0.06. Values represent means±SEM from 11–35 fetuses isolated from 4–13 rats. All the values were expressed as percents of PBS control.

*p<0.05 v.s. PBS;
#


(A) FS vs. SS plot: Wide selection of monocytes depending on FS/SS properties. (B) Pseudocolor CD16 vs. CD14 plot: Gating to select monocytes depending on characteristic "inverted L" shape. (C) Pseudocolor CD16 vs. HLA-DR plot: Gating to select HLA-DR+ cells and to remove NK cells. (D) Pseudocolor CD14 vs. HLA-DR plot: Gating to exclude B cells (HLA-DRhigh/CD14<su


Abbreviations: BMI, body mass index. HR, hazard ratio. Lines are the hazard ratios (areas the 95%-confidence intervals) derived from Cox's proportional-hazard models where BMI was included as restricted cubic splines (3 knots). Reference points are the mean BMI. Years since the examination in 1999–02 was used as time axis. Adjustments: years between examinations, age in 1999–02, chronic diseases, smoking habits, Mediterranean diet score, energy intake, education, drin


Livers from mice infused 1 minute earlier with 1011 Cy3-rAd5 particles were fixed, sectioned (5 µm), and examined by 4-color fluorescence microscopy using mab anti-F4/80 to label KC; anti-MR to identify LSEC; and DAPI to label nuclei. Cy3 fluorescence identified virus. Images were collected and presented as described in legend to Fig. 3</


*calculated based on a comparison with a calmodulin (17 kDa) model, which has a volume of 34.3 nm3.

**calculated from the atomic coordinates of the crystal structure using CRYSOL [65].


To determine the putative catalytic residue in an enzyme, we first select each residue in the structure, one at a time, as a reference residue. Then we regress rate against the set of distances from the reference residue. The putative catalytic residue is the reference residue that maximizes the model’s R2. We have plotted here the distribution of distances of these putative catalytic residues to the nearest known catalytic residue. A distance o


(A) HL-7702 cells transfected with an empty control or Myc-RAD6 expressing plasmids were lysed and subjected to RT-PCR and western blot assays. (B) HL-7702 cells transfected with control or RAD6 siRNAs were lysed and subjected to RT-PCR and western blot assays. (C) HL-7702 cells transfected with an empty control and Myc-RAD6 plasmids together with or without CCND1 siRNA were harvested and stained with PI, and then cells were subje


(A) Representative GLO1 staining of cervix, endometrium and ovary tissues (scale bar, 50 μm). (B) GLO1 IHC score comparison between cervical cancer and normal tissues. Statistical analysis of data was performed using the t-test (**** P<0.0001).


A. Biosynthesis of A. fumigatus siderophores. B–E. C57BL/6 mice infected intrastromally with A. fumigatus mutant strains ΔsidA, ΔsidC, ΔsidF, ΔsidD, or ΔsidG. B. CFU per eye at 4 h and 48 h post-infection. C. Representative eyes showing corneal opacity. D. Quanti


N6-methyladenosine (m6A), the most prevalent post-transcriptional RNA modification throughout the eukaryotic transcriptome, participates in diverse biophysiological processes including cell fates, embryonic development and stress responses. Accumulating evidence suggests that m6A modification in neural development and differentiation are highly regulated processes. As RNA m6A is crucial to protei


Majestic +971509101280 Ｃall ｇirl dubai By Dubai ｃall ｇirls number (W3)

Majestic +971509101280 Ｃall ｇirl dubai By Dubai ｃall ｇirls number (W3)

Majestic +971509101280 Ｃall ｇirl dubai By Dubai ｃall ｇirls number (W3)


The physical properties of polymer\nfilms of nanoscale thickness\nhave been shown to exhibit film thickness-dependent behavior, largely\nassociated with intermolecular interactions between the film and its\nexternal interfaces. These effects are typically manifested in the\nthickness dependencies of the glass transition temperatures (Tgs), and segmental dynamics, over length scales\nranging from a few nanometers to tens of nanometers in many polymer/subs


(E,E,E)-1,6-Diaryl(Ar)-1,3,5-hexatrienes (2, Ar = 4-fluorophenyl; 3, Ar = 2,4-difluorophenyl; 4, Ar = 2,4,6-trifluorophenyl; 5, Ar = perfluorophenyl) and (E,E,E)-1-perfluorophenyl-6-phenyl-1,3,5-hexatriene (6) were\nprepared. The absorption and fluorescence spectra in methylcyclohexane solu


High-valent\niron imido species are implicated as reactive intermediates\nin many iron-catalyzed transformations. However, isolable complexes\nof this type are rare, and their reactivity is poorly understood.\nHerein, we report the synthesis, characterization, and reactivity\nstudies on novel three-coordinate iron(IV) bisimido complexes with\naminocarbene ligation. Using our recently reported synthetic\nmethod for [LFe(NDipp)2] (L = IMes, 1; Me<sub


The\nself-assembly of two novel metallosupramolecular complexes of the\ngeneral formulas [L2M2(CH3CN)4][BF4]4 (M = Co, 1a; M\n= Ni, 1b), where L stands for the tetradentate ligand\n3,5-bis[4-(2,2′-dipyridylamino)phenylacetylenyl]toluene, is\nreported together with their molecular structures ascertained by single-crysta


Mannich-type reaction\nof methyl phenylacetate with the N-tert-butylsulfinyl imine derived from\n(R)-tert-butylsulfinamide and (Z)-14-(pyridin-3′-yl)tetradec-11-enal has been used\nas the key step in the first asymmetric synthesis of (−)-nakinadine\nA. Both the 2,3-syn- and 2,3-anti-diastereoisomers were prepared; comparison of spectroscopic and\nspecific rotation


2-Aminophenol 1,6-dioxygenase (APD)\nis an extradiol dioxygenase\nresponsible for the ring cleavage of 2-aminophenol (2AP) at the position ortho to the hydroxyl substituent. To elucidate the reaction\nmechanism, we conducted quantum mechanical/molecular mechanical (QM/MM)\ncalculations. The mode of binding of the substrate (monodentate or\nbidentate) to the iron center was found to have a crucial role in\ndioxygen activation. The Fe–O2 adducts with 2AP\n


A type\nII polyketide synthase biosynthetic gene cluster (nap) was identified in Streptomyces eurocidicus CGMCC 4.1086 via genome mining. The heterologous expression of the\ncryptic nap gene cluster in Streptomyces\nalbus J1074 generated dimerized aromatic polyketide\nnaphthocyclinones (1–3), whose structures were\ndetermined via extensive analysis using nuclear magnetic resonance\nand high-resolution electrospray ionization mass spectrosc


A series of novel nickel(II) methyl pyridine complexes based on β-ketiminato and phenoxyiminato ligands, [(2,6-iPr2C6H3)NCH(8-R1C10H7)O]Ni(Me)(Py) (5a, R1 = phenyl; 5b, R1 = norbornyl), [(2,6-iPr2C


Di(carbene)-supported\nnickel species 1 and 2 are efficient catalysts\nfor the room-temperature reduction of CO2 to methanol in\nthe presence of sodium borohydride. The catalysts\nfeature unusual stability, particularly for a base metal catalyst,\nenabling >1.1 million turnovers of CO2. Moreover, while\nother systems involve more expensive reducing reagents, sodium borohydride\nis inexpensive and easily handl


Kaplan-Meier plots showing overall survival in lung adenocarcinoma. In red: patients with expression above the median and in black, patients with expressions below the median. CK2α, p = 0.6008; CK2αP, p = 5.6 10−9, CK2α', p = 2.6 10−14; CK2β, p = 8.7 10−8.


This is a summary table of each calculation from three different comparisons of Sesamum vs.Olea, Sesamum vs. Nicotiana, and Sesamum vs. Panax. The rps 12 gene is included in the LSC region. Abbreviations: NG, The numbers of genes; LD, the length differences; IE, the indel events; NP, the numbers of polymorphic sites; ND, the nucleotide differences; Ks, the synonymous substitution differences; and Ka, the nonsyno


Co-expression (black column) and mixture (grey columns, CFP emission: YFP emission) of CFP and YFP yielded no FRET (A). Fluorescent spectra of YFP-GAFA-CFP after excitation at 436 nm, before (dotted line) and after addition of 500 µM cGMP (B). The ratio of intensities in the CFP channel and FRET channel decreases after addition of 500 µM cGMP, indicating an increase in FRET (C). Concentration-response curves of YFP-GAFA-CFP for cG


1
“Genetic lineage” designation is based on AFLP [33] and/or DArT analyses [34], [35].</p


Solid black circles represent the six most frequently studied infectious diseases; open black circles represent three chronic diseases that are often part of the natural course of infection with HBV, HCV, or H. Pylori. Gray diamonds represent the four most frequently studied diseases overall. Gray squares represent the four diseases with the greatest morbidity worldwide; the fifth is HIV/AIDS, represented with a blue dot. ⊕


(A) Schematic representation of the human β-globin pre-mRNA, as in Figures 3D and 4D. The two pairs of arrows represent the coordinates of both amplicons obtained in the qPCR reactions: intron1-exon2 and exon2-intron2 amplicons. (B)


Effects of LAAO isolated of Bothrops leucurus venom on perfusion pressure (A), glomerular filtration rate (B), urinary flow (C), sodium, potassiun and chloride (D, E, F) tubular transport percentage. LAAO-Bl was added to the system 30 min after the beginning of each perfusion. The data represent the mean ± S.E.M of at least six independent experiments. *Significantly different from control group (p<0.05, one way ANOVA, Bonferroni post-test).</


(A) Bone marrow cells were cultured in vitro for 6 days with M-CSF, IFN-γ, and hen egg ovalbumin (50 µg/ml) or vehicle (PBS). Cells were then combined in vitro with CFSE-stained OTI or OTII cells in a 1∶4 ratio (T cells : APCs) for 5 days. (B) Wild-type mice were immunized with GA/IFA (2 mg) for 20 days. Splenocytes were isolated and CD8+ T cells were purified by magnetic bead sorting and stained with CFSE. Bone-marrow monocyt


Blue shaded area marks the number of phosphoproteins specifically identified in one strain. Pink shaded area makes shared phosphoproteins in two strains, while green area labels the common phosphoproteins identified in all three strains. H-G: glycerol cultured Δhog1 strain; W-G: glycerol cultured WT strain; W-M: methanol cultured WT strain.


These data represent, or are calculated from, experimental data. Under References only first authors are listed. Wyszecki (55), (56), and (57), refer respectively to Wyszecki & Stiles (1967) Konig-type fundamentals, Vos & Walraven (1978) fundamentals, and Stiles (1953, 1959) field sensitivities for π1, π4, and π5 mechanisms. In Fuld (1991), and in Kulp & Fuld (1995), data are given at large (20 nm) interval


Ribbon representation of (A and B) PAK1 and PAK1Tpo423, (C and D) PAK2 and PAK2Tpo402 and (E and F) PAK3 and PAK3Tpo436 structures. Ribbon view color code: N-lobe, C-lobe and activation loop are shown in light blue, white and lime green colors, respectively. Gly-loop is shown in orange red color, while phosphorylated (Tpo423,Tpo402 and Tpo436) and unphosporylated (Thr423, Thr402 and Thr436) residues are shown in sandy


A Subset of the sequence similarity network from Fig. 4, with nodes colored according to the identity of the amino acid predicted to occcupy the position of the cis-proline at the N-terminus of beta strand 3 in the Trx fold (Pro 75 in human Trx 1). The orange path traces transitive sequence similarity relationships be


Time evolution of backbone RMSD (A) and ligand RMSD (B) of CYP24A1 models. CYP24A1apo (blue), CYP24A1ketaconozole (green), CYP24A1CTA-091 (black), CYP24A1CTA-018(pink). Eigenvector index versus Eigenvalue index for the first ten eigenvectors of the VDR model (C) and Projection of the motion of CYP24A1 models (D). CYP24A1apo (purple), CYP24A1ketaconozole</sub&g


Our measure of disease-related uncertainty is the infectious disease equity market volatility developed by Baker et al. (2020) [9]. Using sophisticated textual analysis, Baker et al. (2020) [9] search for news articles related to


(A) False-color graph of model predictions for the nPC’s dependence on wavelength (x-axis) and time (y-axis) for settings with, or without, periods of darkness between wavelength steps. All other predictors (basic model) are held constant at their average. Red dots show the peak nPC value for each second, the value right next to it the respective peak wavelength. Green dots and values show the respective trough for nPC. The trough is at 700 nm where


The consensuses identified in Mayetiola destructor are marked by triangles. Bootstrap values less than 50% are eliminated. The tree is built by the ML method (model HKY85) with a bootstrap of 1000 repetitions. The colored triangles represent the hAT lines identified in Mayetiola destructor.

(DOCX)


Background

Effective antiretroviral therapy (ART) has improved the life expectancy of people with HIV (PWH). However, this population is now experiencing accelerated age‐related comorbidities, contributed to by chronic immune activation and inflammation, with dysbiosis of the gut microbiome also implicated.

Method

We conducted a systematic literature search of PubMed, Embase, Scopus, Cochrane reviews and international conf


A: Experiment 2 results. B: Experiment 3 results. Mm = male participants presented with male faces. Mf = male participants presented with female faces. Fm = female participants presented with male faces. Ff = female participants presented with female faces.


Note. Significance levels are indicated as *** p < .001, ** p < .01, * p < .05, ns = not significant. For post-hoc results: S = strong, M = medium, W = weak, N = none, T1-7 = tapping section 1–7, >>> = significantly greater (p < .001), >> = significantly greater (p < .01), > = significantly greater (p < .05), ∼ = not significantly different.

(DOCX)


The analysis of CUR-2GE, CUR-1GE, curcumin was performed on the Waters Acquity UPLCTM H-Class system (Waters Corporation, MA, USA) equipped with a quaternary pump, column oven, autosampler, and photodiode array detector. The samples were separated on Acquity UPLCTM BEH C18 1.7 μm, 2.1 x 50 mm column (Waters Chromatography Ireland Limited, Dublin, Ireland) at 33°C. The mobile phase consisted of 2%v/v acetic acid in water (A) and acetonitrile


Electrophoretogram of amplified carbapenem resistant genes from K. pneumoniae isolates, (A) 16sRNA, (B) Int1, Int2, Int3, ISCR1, (C) KPC, VIM, NDM, OXA-48.


Wildtype and nup116Δ cells were transformed with empty vector or a NOT4 overexpression construct. Cells were grown to log phase at 30°C and subjected to FISH. (A) FISH was performed on cells as described in Materials and Methods. Panels are shown for poly(A) RNA and DAPI to visualize chromatin. (B) Quantification of cells showing nuclear


Pearson correlation analysis of A, B) rPAN-BXA thermal stable Tm values vs. BXA EC50s, or C, D) in silico derived docking scores vs. BXA EC50s. The virus, docking mode, Pearson correlation coefficient, and statistical significance is listed in each panel.


(A, B) Total ROS levels in C57BL/6 x 129 BMM infected for 30 min with opsonized WT, Δgp63, or Δgp63+gp63 L. major NIH S promastigotes were measured by flow cytometry using CellROX Deep Red Reagent. (A) Representative histograms are shown. (B) Quantification of the mean fluorescence intensity (MFI) of each condition relative to the uninfected control is shown. Data are presented as the mean ± SEM of values from t


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We investigate the role of axial ligands on the near-IR-optical and paramagnetic NMR spectra of the complex\n[YbL]+3 where L is the stereodefined enantiopure chiral macrocycle (L = hexaazapentacyclo[25.3.1.112,24.04,9.019,24]dotriaconta-1(31),2,10,12,14,16(32),17,25,27,29-decaene). The conformation in solution of the lanthanide complex\nis character


Five enantiopure palladacycles containing palladium bonded to a stereogenic carbon and an\nN-coordinated oxindole were synthesized by the reaction of alkenyl aryl triflates 2 and 9 with Pd(0)\nbisphosphine complexes. Two palladacyclic complexes, 3β and 10α, were characterized by single-crystal\nX-ray crystallography. The reactivity of neutral palladacycles 3<b&g


Peroxynitrite decay in weakly alkaline media occurs by two concurrent sets of pathways which are distinguished\nby their reaction products. One set leads to net isomerization to NO3- and the other set to net decomposition to\nO2 plus NO2-. At sufficiently high peroxynitrite concentrations, the decay half-time becomes concentration-independent\nand approaches a limiting value predict


Pyrazolo[1‘,5‘:1,6]pyrimido[4,5-d]pyridazin-4(3H)-ones and their analogues, potentially useful for the\ntreatment of erectile dysfunction, were synthesized and evaluated as inhibitors of phosphodiesterase 5 (PDE5).\nSeveral compounds showed IC50 values in the low nanomolar range, and in particular, compound 5r,\ndisplaying high potency toward PDE5 (IC50 = 8.3 nM) and high selectivity versus PDE


High specific capacity materials that can store potassium\n(K) are\nessential for next-generation K-ion batteries. One such candidate\nmaterial is phosphorene (the 2D allotrope of phosphorus (P)), but\nthe potassiation capability of phosphorene has not yet been established.\nHere we systematically investigate the alloying of few-layer phosphorene\n(FLP) with K. Unlike lithium (Li) and sodium (Na), which form Li3P and Na3P, FLP alloys with K to form K


A series\nof next in class small-molecule hepatitis C virus (HCV)\nNS5A inhibitors with picomolar potency containing 2-pyrrolidin-2-yl-5-{4-[4-(2-pyrrolidin-2-yl-1H-imidazol-5-yl)buta-1,3-diynyl]phenyl}-1H-imidazole cores was designed based on the SAR studies available\nfor the reported NS5A inhibitors. Compound 13a (AV4025),\nwith (S,S,S,S)-stereochemistry (EC&l


*
Serum samples were obtained in 2010 from rhesus macaques kept at the German Primate Center. Responses to particular ATRs are indicated (X). Column designated “HSV-ELISA”: Results of HSV-ELISA assay (Figure 4).

1 Results after affinity enrichment of IgG using protein A (samples of crude sera produced high background).&l


Comparison between treated and untreated foxes at t3, and between t0 and t3 within each group. Number of treated and untreated foxes at t0 and t3 (37, 33). Range of values for otitis and CGH shown in parentheses above each bar. Foxes excluded from analyses: three treated and two untreated foxes at t0, one treated and untreated fox at t3<


Background

Acinetobacter baumannii, the first human pathogen to be designated as a "red-alert" pathogen, is on the critical priority list of pathogens requiring new antibiotics. Biofilm-associated diseases are the most common infections caused by the antibiotic-resistant bacteria A. baumannii. Multidrug-resistant strains are more easily transmitted around the world due to A. <i&gt


CRISPR/Cas components used and results of (co-) transformations with strain Guy11 and Guy11ku80. Transformations with the same letter were done with the same batch of protoplasts.

(XLSX)


(A-B) Levels of IL-1β and IL-18 measured in serum (mean ± SD of two independent experiments, n = 6 mice, Student’s t-test, *p<0.05 or **p<0.01 indicates the difference from wildtype mice at 6 dpi). (C) Level of anti-Ft LPS IgM antibody titer measured in serum (mean ± SD of OD450 from two independent experiments, n = 6 mice, Student’s t-test). (D) Levels of anti-Ft IgG and IgA antibodies measured in serum (mean ± SD of OD450 from two in


Purpose

We aim to evaluate the efficacy and safety of transcutaneous electrical acupoint stimulation(TEAS) in elderly patients around the perioperative period of hip replacement surgery.

Methods

The China National Knowledge Infrastructure(CNKI), Wangfang Data, VIP database, SinoMed, PubMed, and Embase databases were searched for relevant publications until August 2024. All randomized controlled studies evaluating the effic


This essay deals with the definition of ‘kanji competence’ from the point of view of the three main approaches to language in Japanese: shiru (to know), wakaru (to understand) and dekiru (to be able). After outlining competence, the essay proceeds to investigate how to carry on a comprehensive teaching process which takes into account: ‘who’ (are the learners), ‘what’ (they need to learn) and ‘how’ (to teach). The cons


OR: Odds ratio for a 10 μg/m3 increase in each pollutant exposure; 95% CI: 95% confidence interval. ** Risk Ratio and 95% CI estimated using a time series design and Poisson regression models adjusted for mean daily temperature, mean daily absolute humidity, seasonality, and day of the week.

(PDF)


(a) ELISA demonstrating the ability of CsgA-αgp900-2 to bind gp900. PBP8 was adsorbed onto a well plate, followed by incubation with gp900-containing C. parvum lysate. In the positive control, no PBP8 was used, and the C. parvum lysate was allowed to directly adsorb onto the surface of the wells. Gp900, either bound to PBP8 or adsorbed to the surface, was detected using a non-competing anti-gp900 VHH, followed b


We analyzed the behavior of three different groups: control (grey), anti-vaccination (red) and pro-vaccination (blue) (A). We calculated the number of tweets, replies and retweets per month (B). The anti-vaccination group scored the highest number of total Twitter actions (the sum of tweets, replies and retweets) per month (C). Anti-vaccination supporters tweeted less than control and pro-vaccination individuals (D</b&


Comparison of the expression of NFKBIA, NFKB-1, TBK-1, IL-10 and TNF-α in DCs in the presence of the probiotic (Prob), its supernatant (CFS), Salmonella (S.typhi) or a combination. LPS, 20 ng/ml, was used as a positive control. The fold change (Fc) represents the ratio of the expression in treated DCs to the expression in control cells. The data shown are the mean values and SEM of three independent experiments.


Values represent the percentage of recording time within each solar elevation bin during which song sessions were detected. This analysis pools all November through January data from the first three years of recordings (Fig 4), when 76% of all song was detected (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0222456#pone.0222456.g005"


(a) A small, randomly selected region in the distal membrane is represented as a collection of particles (small circles). Each particle represents a collection of lipid molecules, and is located at the center of a circular neighborhood (Nx). The motion of an arbitrary particle x (in yellow) at the center of Nx is influenced by the motion of every particle in Nx via bonds. If no forces apply to the membrane, the particl


The set-point of A is Aset=3.0. (a) Step-wise increase of k1 from 1.0 to 5.0 at time t=100 at different and constant background perturbations k3 (0–80). Note the successive decrease in the excursion of A (ΔAmax) and the more rap


Objectives
To investigate the clinical value of hemodynamic parameters derived from dynamic contrast-enhanced MRI (DCE-MRI) in predicting glioma genotypes including isocitrate dehydrogenase (IDH) mutation, 1p/19q codeletion status and the tumor proliferation index (Ki-67) noninvasively. And to compare the diagnostic performance of parameters of distributed parameter (DP)model and extended Tofts (Ex-Tofts) model.
Materials and methods
Dynamic contrast-enhanced MRI (DCE-MR


七月三十一日午後一時十五分倫敦発東京海上保険着電は前日の発電に比し左の如き暴騰を伝えたり

英国船(蘇士及び喜望峰経由共英国の港を目的とする直航の場合英国所属船百磅に付三磅)三倍に騰貴
英国船(若し本船と英国所属に限る…が欧洲大陸へ寄港したる時又は欧洲大陸より往復の時は百磅に付五磅)二倍半に騰貴
独伊両国船(百磅に付十磅)二倍半に騰貴
仏露両国船(百磅に付五磅)四分一騰貴
其他の国船(百磅に付四磅)据置き

而してカルカッタ、海峡殖民地、支那、日本航海の汽船積貨物は独伊露仏の四ヶ国船を除ける他の汽船は百磅に付二磅と変りなけれど仏露両国船は百磅に付四磅(二倍)独伊両国船は百磅に付八磅(四倍)となりたり墺国船は是等凡ての場合を通じて除外さるるものなるが以上海上保険料…戦争危険のみの保険料の暴騰を以て見れば欧洲の風雲刻々に重大視せられつつある事明らかなると共に前日迄同様に取扱われし露仏独伊の四ヶ国船の保険料が三十一日発電の場合に於て独


一

 わが国産業が科学との結付きを絶対必要とする理由は、特に戦争遂行乃至戦争準備を前提としてその機構の改編を試みるにおよんで、一層重要意義を加えて来た。すなわち(一)生産力拡充の促進(二)代用資源確保(三)国防兵器の製造のため、わが産業なかんずく軍需工業はその科学的要素を百パーセント緊急動員し活動せしめなければならない。かの欧州大戦に際して、ドイツが発明した空中窒素固定法の如きはかかる意味における代表的な成果の実例として教えられるところが多い。しかしながら産業の「科学化」はその様相、機構において常に移動する。科学の進歩に伴い目まぐるしく更改されつつある、しかも資材戦を中心とする近代戦との関連において、その技術的基礎は次第に機密化され厳重なる秘密裏に列国は競って新しい科学的研究に没頭している、かかる環境にあってわが産業は今や一刻も早く日本独自の科学的基礎を確保し、これに対抗しなければならない。わが国においても近時その緊要性を認め、この種研究機関が漸次設立されるに至ったが、その現状は次の如くである。すなわち<br/&gt


（上） 糖業家と投機業者への脅威

台湾における各製糖会社は近年同地における内地種米の移植が販売価格においてと、収獲の量に於ても甘蔗栽培に比し遥に有利なので作付反別が急激に増加して来たこれやがて砂糖生産費に大影響を及ぼすものでなければならない、即ち台湾在来米と内地種の米との七日東京市場における相場を見れば

 （・点円）
 在来米 百斤 一一・〇〇
 内地種 同 一五・三〇

内地種が四円三十銭方の上鞘にあり、また収獲量を比較すれば（・点石）

 在来米 一甲歩 一七・七九四
 内地種 同 一八・四六八
「註」十三年度一期作の台中州内各地に於ける平均比較である

右の如く計数的に内地種の有利を表示しておる、勿論内地種が比較的良田に移植されるに反して在来種が水田の良否に拘らず植培されるにしても兎に角収獲量において


株式奪合の経過

日本染料製造株式会社創立委員会は二十六日大倉事務所に開かれ発起人を確定したること既報の如くなるが問題たりし社長及び一万五千株の賛成株分配方法等に就ては所謂プレミアム憧憬派と純公募派との意見一致せず之を各発起人の意思に一任する時は到底纏まり付かざる次第なるを以て中野大倉両氏に一任することとなり二十七日中野、大倉、植村久米等の諸氏は岸博士等と会合して分配問題に就きて種々協議を重ねたるも猶決定を見るに至らず孰れ最近に於て此の問題を定め愈々公募の手続きに及ぶこととなる可しと云う

プレミアム糶上げ

而して斯く賛成株の奪い合を為すは全くプレミアム附発行を予想して権利株の利益を獲得せんとするにあるは云う迄も無き次第にして政府より十年間八朱の保護を為すとの理由によりて最初は三五円のプレミアムを予想せられしものが今日にては所謂お祭り騒ぎを利して事を為さんと或種の仲買商等によりて十円十五円と糶上げられ未だ株式分配の方法並に公募方法共に何等の決定をも見ざる今日なるにも


This investigation looks at the cellular make-up of normal, acute and chronic conjunctiva with particular reference to the role of the mast cell. Conjunctival biopsies were taken from the upper tarsal plate and bulbar conjunctiva,

Inflammatory cells were found to be present in the tissues in varying numbers, distribution and location. Eosinophils are not normally found in normals but in this study a few subjects were found to have them in the substantia propria, the re


Nel Trecento e Quattrocento, la riflessione teologica ed etico-economica fondata sui concetti di bonum commune, di interesse, di mercato (sviluppata soprattutto dai francescani) arriva gradatamente a definirsi come un “sistema” economico cittadino.

The theological and ethic-economic thought, based on such concepts as the bonum commune, interest and personal responsibility (that the Franciscan friars elaborated), progressive


最近英国造船界は経済状態の不安及び労働問題の紛糾、労銀の昂騰労働時間の短縮、造船材料の不足等より其発展を阻害せんとする形勢にして造船業者は孰れも前途を警戒して新造船の註文を回避するに至れり之れが原因を挙ぐれば左の如し

一、経済界の前途不安にして之れが予測困難なること
二、政府の利得課税に対する態度の不明なる為め目下新造船の契約を為す場合は其新造船完成する頃に於て如何なる課税を実施せらるるや明かならず而して此課税が造船業者の利益に直接重大なる影響を来すべく危惧され居ること
三、労働賃銀の昂騰せること。造船工及び機関工組合の一時間六片一週一磅三志六片の賃銀値上げ要求に関し工業法定に於て認可せらるる場合は製鋼工のみならず造船工全般の労銀値上となり造船費に多大の影響を来すべきこと
四、造船材料の不足せること。現に鋼鉄の如きは生産不足を来して米国より其一部を輸入し補填しつつあるも米国が鉄道輸送力の停滞により捗々しく輸入し得ざること
五、海運界の情勢不振の為め船舶の需要著しく減


発行権の譲渡

政府は満鮮の経済的統一を図るを其の政綱の一とし此方針の下に種々の施設をなしたるが今回また同一の趣旨に依り満洲に於ける正金銀行の金券発行の特権を朝鮮銀行に譲らしむるに決したり元来正金銀行は勅令に依り大正二年七月より同七年七月迄五箇年間満洲に於て金券を発行する特権を賦与せらる居るが故に明年七月迄は尚金券発行の特権を有すれども政府が満鮮の経済的統一方針を徹底する便宜上、十一月三十日限り金券発行の特権を鮮銀に譲渡すこととし目下満洲に流通しつつある正金の金券は十二月以降鮮銀の金券と同一に取扱い鮮銀にて其の引替えに応ずべし因に目下満洲に流通し居る正金発行の金券は約四百五十万円なり

金庫事務譲渡

従来日本銀行より正金に委嘱し居りし金庫事務も鮮銀に譲渡することとなり十二月三十一日限り引渡の手続を為すべし但し満洲意外の支那に於ける金庫事務は依然正金にて取扱う

銀券発行依然

正金銀行は


Context Reduced neural responses to others' distress is hypothesized to play a critical role in conduct problems coupled with callous-unemotional traits, whereas increased neural responses to affective stimuli may accompany conduct problems without callous-unemotional traits. Heterogeneity of affective profiles in conduct problems may account for inconsistent neuroimaging findings in this population.
\n\n
Objectives</strong&


(一) 農夫と労働者

欧洲大乱の苦悩を親しく嘗めて来た結果として戦前よりも仏蘭西は英雄に成った又誰の眼にも従来より偉く見える様になって来た然し乍ら幸か不幸か仏国はそれが為めに非常なる負担を将来に貽すの止むを得ざるに至ったのである世界大戦の為めに仏国は第二の国民として将来世界の創始者たり産業界に於ける生産者たるべき多数の青年子弟を奪い去られて了った国内に於て戦禍を蒙らざるものとては一つもなく仮令生き残った青年と雖も北部や東部に於ける頽廃せる鉱山や殆んど再起の余地なき迄に破壊されたる工業を以てしては殆んど当分手の付けようがないのである仏国民は多年勤倹の結果貯蓄したる多くの財宝を戦争のために消費し尽し戦前の沃土は鉄蹄の蹂躙する所となって亦昔日の面影無く仏国民は現在は勿論のこと将来に於ても多大の制肘を其発展上に受くるに至ったのである
莫大なる資本を戦争のために蕩尽し去った結果として戦後の復旧事業や改造事業と雖も却却容易の問題ではない経済界の変動の結果として仏国は其輸入品に対しては戦前其全盛時代に支払って居たものの約五倍の金を支払わな


【上海特電五日発】過般来紛擾していた上海船員の同盟罷業問題につき招商局は第三回要求書に返答を与えなかったため船員は五日午前七時からストライキを開始し招商局汽船五隻は繋留されたまま航海を中止するに至った、罷業は同会社の他の汽船にも及ばんとする形勢がある、招商局の汽船は総て三十四隻で罷業が全部の汽船に及ばば支那の内河航は大恐慌を来すだろう

双方の主張

【上海特電六日発】五日来開始された上海の船員罷業は六日迄に罷業船数十三隻、人数一千人に達し益々拡大の模様あり、船員側は五日付上海船員全体の名義で罷業の理由を中外に宣言した、船主側招商局当局は語る
船員側の要求は余り過大である、即ち(一)賃金二割を増加する事(二)香港の例に倣い本年一月に遡って増給する事であるが之を実行すると船主側の損失は莫大で目下香港に碇泊中の船を除き上海に在るもの三十隻、毎隻五十人とするも千五百人に達し二割を増加するとすれば合計十二隻元を要し一月に遡って計算すれば八十隻元を要する、工業不振の際斯かる莫大な金額は到底支給し難


钢材具有强度高、材质均匀、塑性韧性好、质量轻、制造方便等优良性能，一直是建筑结构的重要材料。随着这些年来我国年钢产量的迅速增长和建设部大力推广钢结构在多、高层民用建筑中应用的政策发布之后，钢结构这种建筑结构形式在我国的高层建筑中的应用也随之增长迅速。
\n交错桁架结构体系是在钢框架结构的基础上发展而来的一种新型结构体系，适用于高层民用建筑。交错桁架结构体系是由平面桁架、柱、连梁、楼板组成的空间结构体系，传统的交错桁架结构柱距较大，这样有利有弊，在得到比较大的房屋面积的情况下也减小了结构的纵向抗侧刚度。传统交错桁架结构的纵向抗侧刚度比横向抗侧刚度小很多，这大大减小了结构的抗震性能。若要提高侧向刚度需要增大梁和柱的截面，但会使得用钢量大大增加，在结构纵向布置柱间支撑是更经济合理的方式。本文主要研究内容：
\n（1）为了提高结构的纵向刚度，在结构的纵向布置四种不同形式的柱间支撑：单斜支撑、X型支撑、V型支撑和人字型支撑。使用PKPM的PMSAP版块对这四种模型进行建模分析，选出位移最小且最经济合理的布置形式是人字形支撑。并对人字型


赴任後初帰りの伊集院関東長官は二十九日朝下関に帰着、同十時五十分門司発鹿児島に帰省したが一月初旬状況の筈である、長官は語る
明年度予算は既に発表された如く要求額に多少の削減を見たが前年度に比し大差なく先ず之ならば無事だと信ずる、併しこの影響により繰延べとなった事業は管内電柱建替え工事の繰延べが先ず主なもので他はいうべき程のものもない

警官増さぬ

明年四月から遼陽以南の独立守備隊撤退開始につき之に代る治安維持を如何にするかが問題であるが現在の警察官を以て当る外なく警官の増員はせぬ事になって居る、五十名の警察官増加が明年度の予算に計上されているが之は定期補充のための増員で守備隊撤退と関係はない、併し現在の数だけで治安維持に支障はない、若し多数応援の必要が起った場合は満洲駐屯軍を以て之に充てる計画である

財界救済策

満洲財界救済については永遠と当座の二方針を樹て先ず当座の救済としては例の東拓の三百六十万円貸


小運送の改善発達を計るため、官民合同の調査会は既に一般調査を完了してこれが対策を決定すべく特別委員会を設け
村井鉄道省運輸局長を委員長に和田東京市電気局理事、服部商業会議所書記長、中野内国通運社長、谷本三菱倉庫常務、安田郵船営業部長、塚田東武鉄道専務及び鉄道省側から新井業務課長、中山配車課長、工富東鉄運輸課長、吉田貨物課長等
委員として協議を進めつつあるが小運送の欠陥としては

一、小業分立の結果料率不定である事
二、従って料金は鉄道貨物運賃に比して最高六倍から二三倍に当る事
三、競争の結果荷主の歓心を買わん為め貨物引換証の濫発、即ち空券発行の弊ある事
四、引受貨物に対して責任の完全を欠く場合ある事

その他種々あって現行制度は幾多の弊習が伴い、国家経済上の不利不便は少くない、で特別委員会における対策の綱要として議にのぼって居るものの中では

一、運送取扱業の監督は鉄道


A numerical analysis of the phytosociological relevés from ancient and recent woodland on a mesic forest site in central Poland was carried out. Three groups of forest stands were recognised: planted with Pinus, recent and ancient oak-hornbeam Tilio-Carpinetum. The species of ancient woodland occurred mainly in ancient oak-hornbeam forest stands, however some ferns: Dryopteris carthusiana, D. dilatata and <em&


Driving through intersections can be potentially dangerous because nearly 23 percent of the total automotive related fatalities and almost 1 million injury-causing crashes occur at or within intersections every year [1]. The impact of traffic intersections on trip delays also leads to waste of human and natural resources. Our goal is to increase the safety and throughput of traffic int


Physiological and symbiotic properties of Bradyrhizobium sp. (Lupinus) nodule isolates were compared to the standard slow-growing Bradyrhizobium sp. (Lupinus) strain USDA 3045. Lupine nodules isolates showed typical characteristics for bradyrhizobial strains and nodulated small seed legume, serradella (Ornithopus sativus), in tube test. We observed a permanent physiological segregation of the effective


In this paper we present and analyse new CCD <it>UBVRI</it> photometry down to <it>V</it>&ap;21 in the region of the young open cluster Trumpler 15, located in the Carina spiral feature. The cluster is rather compact and has a core radius of about 2 arcmin, which translates to about 1 pc at the distance of the cluster. We provide the first CCD investigation and update its fundamental parameters. We identify 90 candidate photometric members on the base of the position


This article takes issue with Walter Benjamin’s thinking pattern and his pursuit of dialectical method. The limits of this study are determined through his relationship with other Frankfurt school members, studies on history, and especially, through the character of flâneur. Although Benjamin’s dialectical approach does not obey the classical Marxist dialectics, he actually developed the dialectical methods through a unique way. It can be thought that his


* Samples used in Nei’s genetic diversity estimation at haplotype level reported in Table 3.

Mitochondrial haplogroup, biological sex, and age of 29 individuals recovered from Teopancazco. The HVRI sequence from 16 of them is also shown (positions 16190–16339). Mutations are numbered according to the revised Cambridge Reference Sequence [<


The volume of intercellular spaces in leaves at various stages of water saturation was determined by method of Czerski (1964, 1968). The investigation were performed with the following plant species: Vicia faba L., Nicotiana tabacum L. var. rustica, Solarium tuberosum L. var. Flisak, Helichrysum bracteatum Wild., Bmssica napus L. var. oleifera, Beta vulgaris L. var. sacchari


Purpose: To assess the clinical application of multicolor imaging by confocal scanning laser ophthalmoscopy (cSLO). Methods: Retinal imaging was performed in 76 patients including cSLO multicolor imaging (SPECTRALIS SD-OCT, Heidelberg Engineering, Heidelberg, Germany) and color fundus photography (CFP). Results: The use of confocal optics, reduced light scatter and automated eye trac


Cadaba indica, a component of traditional siddha medicine, is used for the therapy of gonorrhea, inflammation, eczema etc. The present study shows that it exhibited acetylcholinesterase inhibitory activity (up to 81.5 ± 1.5%) at the concentration of 3 mg/mL, with the IC50 value of 1.8 mg/mL. Furthermore, the ethanol extract of C. indica therapy at a dose of 100 mg/kg p.o significantly ameliorated the cogni


<sec><st>Background</st> Acanthamoeba keratitis<it> (</it>AK) is a serious corneal infection that may even lead to loss of sight. Infection prevalence has increased in recent years as the use of contact lenses (CL) has also increased. </sec> <sec><st>Purpose</st> To analyse if there is a difference in duration and efficacy in treatment with chlorhexidine eye drops 0.02% (CED) in AK alone or associated with <it>Pseudomonas spp</it>


Waarneming tijdens munitiesanering op 21 december 2006 en tijdens aanleg rioolsleuf op 27 april 2007 in Driel-Zuid, Gemeente Overbetuwe.
De saneringsgaten en de rioolsleuf hebben de resultaten van de oudere onderzoeken bevestigd. Tijdens de waarnemingen zijn geen archeologisch relevante sporen en vondsten aangetroffen. Hiermee is de lage waardering die aan dit deel van het plangebied, de komgronden, is toegekend terecht gebleken.


John Naisbitt &amp; Patricia Aburdene,
d a l am pengantar bukunya lentang
Megatrend 2000, menulis bahwa kita sekarang
memasuki era baru, menyongsong
dasawarsa yang sangat penting dalam
sejarah pcradaban manusia. Era yang ditandai
gencamya inovasi teknologi, pcluang
ekonomi yang tak tcrbayangkan scbelumnya,
serta rcvormasi politik yang radikal
dan bcrdampak global.
Era baru y


Abstract

Investment is the initial capital in a country's economic development. Shariah bank in this case BRI Syariah is expected to be able to contribute to the growth of public investment in Indonesia through Sharia Deposits. There is a growing perception in the community that Islamic banks are the same as conventional banks, indicating that there are still many people who do not know about


Background and Aims: Mild cognitive impairment (MCI) represents an important point on the pathway to developing dementia and a target for early detection and intervention. There is a shortage of validated cognitive screening tools in Arabic to diagnose MCI. The aim of this study was to validate Addenbrooke’s Cognitive Examination-III (ACE-III) (Egyptian-Arabic version) in a sample of patients with MCI, to provide cut-off scores in Egyptian-Arabic speakers. &


Instalasi antena parabola berfungsi untuk memudahkan pada saat pengarahan pointing antena ke satelit yang dituju. Permasalahan yang diketahui yaitu bagaimana perilaku parameter Bit Error Rate (BER) dan Carrier to Noise (C/N) pada LNB Combo yang menggunakan teknologi DVB-S2. Setelah instalasi antena parabola dilakukan dan sukses, maka langkah selanjutnya yaitu melakukan pointing anten


通过对于道路桥梁施工技术现状的认识，能够更加清晰看到我国的道路桥梁在未来的发展过程当中，需要集中改进的地方，对于我

国道路桥梁的全面建设和未来发展也提出了许多全新的启示。在科学技术不断应用，土木工程学更加完善的同时，新材料新技术的应用和全新管
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Figure S1. Fish feeding rates over the course of the GHG study period. There was an intensive campaign of Tilapia production from May through August of 2019, resulting in the higher feeding rate. Production tempo slowed starting in September of 2019 and all feeding stopped for approximately the coldest month of the year.

Figure S2. Dissolved oxygen levels (A) and temperature (B) in the fish tank. The boxed area shows the 10 month period of time during the GHG study.&


Rechtsgrundlagen kennen, verstehen und anwenden! Diese Arbeitshilfe Grundlagen - SGB III: Arbeitsförderung enthält den aktuellen Gesetzestext des Dritten Buches Sozialgesetzbuch (SGB III). Die Einführung gibt Überblick über die Rechtsmaterie, erläutert den Gesetzesaufbau, sowie den Leistungskatalog der Arbeitsförderung:
 Berufsberatung, Arbeitsmarktberatung
 Ausbildungs- und Arbeitsvermittlung
 Aktivierung und berufliche Eingliederung &lt


Abstract
Purpose: To identify the individual genes or gene modules that lead to the OncoptypeDx 21-gene recurrence score's reduced performance after 5 years and thereby identify indices of residual risk that may guide selection of patients for extended adjuvant therapy.

Experimental Design: We conducted a retrospective assessment of the relationship between (i) the individual genes and gene modules of the Recurrence Score


Abstract
Background: The effects of use of different types of hormone therapy on breast cancer risk according to prognostic factors are largely unknown.

Methods: We linked data from the Norwegian Prescription Database and the Cancer Registry of Norway during 2004 to 2009 on all women ages 45 to 79 years (N = 686,614). We estimated rate ratios and 95% confidence intervals for breast cancer in relation to


Abstract
Purpose: Intrahepatic cholangiocarcinoma (ICC) is the second most common primary cancer in the liver, and its incidence is increasing in developed countries.

Experimental Design: To discover novel molecular targets for the diagnosis and treatment of ICCs, we earlier analyzed expression profiles of 25 ICCs using a cDNA microarray containing 27,648 genes. In this study, we focused on the RAD51 associating protein-1


Figure S5 shows Casp3 levels through cell cycle phases, protein levels of full length and cleaved CASPASE 9, CASPASE 2, PARP-1, and fraction of live, dead, and apoptotic cells in asynchronous and G2-phase synchronized PDAC cells, quantification of full length and cleaved cPLA2 and CASPASE-3 in asynchronous and G2-phase synchronized PDAC cells, expression of wild type cPLA2 and the CASPASE-3-cleavage resistant cPLA2 mutant in endogenous cPLA2 knockdown human PDAC cells


A series\nof dialkylated dinaphtho[2,1-b:2′,1′-f]thieno[3,2-b]thiophenes (S-DNTTs) featuring\nan S-shaped sinuous π-core substituted with different alkyl\nchains were developed as versatile p-type organic semiconductors.\nThe effects of the crooked shape of the π-core and the length\nof the flexible alkyl chains on the solution processability, thermal\ndurability, self-organization ability, and charge-transport functionality\nwere syst


Photoinduced phase\nseparation in mixed halide perovskites emerges\nfrom their electro-mechanical properties and high ionic conductivities,\nresulting in photoinduced I<sup>–</sup>-rich charge carrier\ntraps that diminish photovoltaic performance. Whether photoinduced\nphase separation stems from the polycrystalline microstructure or\nis an intrinsic material property has been an open question. We investigate\nthe nanoscale photoinduced behavior of single-crystal mixed Br<sup>–


A series of novel\n4-aminoquinoline analogues bearing a methyl group\nat 4-aminoquinoline moiety were synthesized via a\nnew and robust synthetic route comprising in situ tert-butoxycarbonyl (Boc) deprotection–methylation\ncascade resulting in the corresponding N-methylated secondary amine\nusing Red-Al and an efficient microwave-assisted strategy for the\nfusion of N-methylated secondary amine with 4-chloroquinoline nucleus\nto access the


A 2:1 lithium amide/n-butyllithium aggregate 1 is investigated as an asymmetric addition template in hydrocarbon solvents. Several different chiral lithium amides were synthesized from l-valine and tested in the asymmetric addition of n-BuLi to various aldehydes. Enantiomeric excesses up to 83% were obtained in the case of the addition of n-BuLi to pivaldehyde at −116 °C in pentane. 1H and <sup&


a) Long-range contacts between IBP motifs, as measured by interaction variable betas estimated using models (2) and (3). b) Long-range contacts between IBP motifs depending on the distance. c) Long-range contacts between IB


<p>(A) Multidimensional scaling plot of expression profiles of human lung epithelial subsets from three patients (a 64-y-old male exsmoker, an 83-y-old male exsmoker, and a 53-y-old male current smoker). Distances represent the leading log2-fold change. (B) Gene ontology (GO) terms associated with DNA repair or the cell cycle are significantly up-regulated in BSCs compared to AT2 cells by rotation gene set tests (ROAST) (<i>p</i> < 0.02). Each pair of bars corresponds to a r


The central circadian clock of the suprachiasmatic nucleus (SCN) is a network consisting of various types of neurons and glial cells. Individual cells have the autonomous molecular machinery of a cellular clock, but their intrinsic periods vary considerably. Here, we show that arginine vasopressin (AVP) neurons set the ensemble period of the SCN network in vivo to control the circadian behavior rhythm. Artificial lengthening of cellular periods by deleting casein kin


(A) Genomic location of the IR genes (black arrowheads; pseudogenes in grey) belonging to the IR94 and IR52 clades in D. melanogaster, D. sechellia, D. ananassae and D. virilis. Equivalent chromosome arms (Muller elements) (labelled on the left of each chromosome arm) between the species are indicated by colour and horizontal alignment <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen


We performed simulations similarly as in Fig 5 but by varying replication rate r or death rate δ independently. We selected trajectories in which the percent of plasmid-bearing cells was above LOD. We show how the total number of bacteria (panels A-B) or the percent of plasmid-bea


Most of the tested isolates of the M. fortuitum group and M. abscessus were unable to form biofilms. The number of isolates in the M. fortuitum group that formed a strong biofilm was higher than that of M. abscessus. Most other species could not form biofilms.


(A) Immunoprecipitation (IP) showing the effect of Mi-2, Mi-3, and Mi-nc on the interaction between Menin and MLL1. (B) Flow cytometry and (C) W.B. showing that Mi-2 and Mi-3 induce the apoptosis of SAOS2 cells. (D) A model of a RUNX2-mediated epigenetic mechanism that regulates MYC expression and the survival of OS cells.


A) Chlorophyll a levels. FW; Fresh weight. B) Photosynthetic rates of 14C fixation. Results are mean ± SE (n = 3). Statistically different results (p<0.05) are marked with a different letter (Analysis of variance). (C–K) DIC, chlorophyll autofluorescence and merged images of day phase cells at day 0 (C–E), day 7 (F–H) and day 14 (I–K). Chlorophyll intensity is highest in the periphery of day phase cells cells (white arrows) due to a separatio


Ants were trained either on an open maze or on a confined maze, and pheromone was either removed or allowed to remain. Note that on the final (7th) visit shown, all ants are tested on an open maze with no pheromone information. The presence of pheromone during the previous training visits had no effect on learning, and confinement caused ants to make more mistakes at the first bifurcation. Symbols represent means, whiskers 95% Cis.


(A) Biotinylated HpaII enzyme is conjugated to streptavidin coated magnetic beads. A DNA mixture can then be added to the conjugated beads and following incubation the mixture is segregated into fractions that are bound (containing majority of Y. pestis) or unbound (containing majority of human) to the beads. (B) Adding salt to the binding buffer enhances segregation of human (blue bars) from Y. pestis (green ba


Aim

Active transportation referring to non-motorized modes of transport is promoted and popularized both in practice and in the scientific literature, while their use for urban freight transport has been largely neglected. Thus the main scope of the paper is to indicate the development potential of micromobility use in urban freight transport and to check its influence on urban sustainability.

Methods

The authors have hypo


Cytotoxicity profile of nine of the eighteen MMV compounds tested against the RAW 264.7 macrophage cell line using the MTT assay. All data shown are the representation of three independent experiments.

(TIF)


This image is a digital reproduction of a black and white photograph donated by William Kattan.

The photograph depicts the Grotte de Massabielle in Lourdes, France where the Kattan family would visit if a member of their family was unwell, as part of a religious pilgrimage.

This image exists as part of the Kattan Family collection within the William Katta


Photocatalysis is a promising advanced\noxidation technology for\ntreating wastewater; however, the complexity, diversity, and variability\nof actual wastewater make its application difficult at the current\nlevel of technology. In this work, a photocatalyst gradation strategy\nis proposed in which the specific photocatalysts Ag2O,\nNaBiO3, and BiOCl0.75Br0.25 are combined\nto treat the specific pollutant


Supplementary cementitious materials (SCMs) are eco-friendly cementitious materials that can partially replace ordinary Portland cement (OPC). The occurrence of early-age cracking in OPC-SCM blended cement is a significant factor impacting the mechanical properties and durability of the concrete. This article presents a comprehensive review of the existing research on cracking in OPC-SCM concrete mix at early ages. To assess the effects of SCMs on the early-age cracking of concrete, the properti


Supplementary materials to “Szastok, M., Kossowska, M., & Pyrkosz-Pacyna, J. (2019). Women can’t have it all: Benevolent sexism predicts attitudes toward working (vs. stay-at-home) mothers. Social Psychological Bulletin, 14(1), Article e29461. https://doi.org/10.32872/spb.v14i1.29461”