Additional file 3: Figure. S3. AC infection causes gastrointestinal motility disorders in mice. (A) Macroscopic images of the mouse intestines. (B) Statistics of first pellet staining for fecal expulsion time. (C) Statistics of fecal pellet frequency within 8 h. (D) Statistics of fecal pellet output within 8 h. (E) Statistics of gastric residual rate. (F) Statistics of intestinal propulsion rate. (G) Statistics of fecal moisture content. Data are presented as mean ± SD, n = 5. Compared to the Co


Additional file 2: Fig. S2. Concept of in vitro assay. Three patterns of how ANCA binds to primed neutrophils (a). Requirement of cellular adhesion for ANCA-induced NET formation (b). MPO-immobilized slide coated with anti-MPO antibodies mimics these situations (c).


Additional file 3: Figure S1. Rank abundance curve of rpL6 scaffolds (centroids for sequences clustered at 97.5 % nucleotide identity) and their taxonomic assignment. Black ticks represent clusters with scaffolds that were binned in genomes. Clusters with < 5 sequences were combined in “others”. The taxonomic affiliation presented here corresponds to that of the Genome Taxonomy Database (GTDB release 05-RS95), while the taxonomy of the genomes was assigned based on phylogenetic placement in a


Additional file 9. Gene Ontology of Relapse-Associated M-CLL network modules. Biological processes (green), molecular processes (blue), and cellular locations (brown) of M6, M10, M11, and M12 modules are displayed on the y-axis. Z-scores are on the x-axis.


Additional file 11: Table S5. Statistics of the neutral model in host and water microbiomes. This table summarises the neutral model fit based on the following parameters; the migration rate (m.ci) within 95% of the confidence interval, the goodness of fit(R2), number of samples, richness, abundance cutoff, percentage of % neutral OTUs and non-neutral OTUs.


Additional file 4. Expression of cell surface proteins in CD45−/ALDHdim cells and CD45−/ALDHbr cells. Sorted CD45−/ALDHdim cells and CD45−/ALDHbr cells were examined for cell surface markers associated with mesenchymal stem cells (MSCs), fibroblasts, and stem cells.


Supplementary material for the publication: Juckes et al. (2024) Baseline Climate Variables for Earth System Modelling, submitted to GMD. This data summarises the WCRP Baseline Variables list, and includes the raw data from throughout the prioritisation process.


Supplementary Material 2: Figure S2. Effects of increased protein lactyation on the proliferative capacity of BC cells. (a). Cell proliferation ability was evaluated by CCK8 assays in MDA-MB-468, MCF7, T-47D, and 4T1 cells after treatment with 0mM, 10mM or 25mM of NaLac. (b). Cell proliferation ability was evaluated by colony formation assays in MDA-MB-468, MCF7, T-47D, and 4T1 cells after treatment with different concentrations of NaLac. Error bars represent the mean±SD, ns: not significant.


Additional file 1. S1. PRISMA checklist. S2. Search strategy. S3. Post hoc subgroup analysis for the comparison of loop diuretics vs. placebo/no intervention. S4. Detailed characteristics of included trials. S5. Overall risk of bias for all included trials. S6. Comparison: loop diuretics vs. placebo/no intervention. S6a. Risk of bias of all outcomes. S6b. Meta-analyses and TSA. S6c. Subgroup analyses. S6d. Sensitivity analyses. S6e. Reported SAEs and AEs. S7. Comparison: loop diuretics vs. anoth


Additional file 21:qRT-PCR validation of the microarray data showing the change in the expression levels of transcripts differentially expressed between MII oocytes and CCs. Transcripts marked by the minus sign indicate those over expressed in MII CCs. (DOC 31 KB)


Additional file 2: Supplementary tables 1-7. Table S1. Primers used for vectors construction. Table S2. Primers used for positive test. Table S3. Barcode primers for detecting off-target in base editing T0 transgenic plants with deep sequencing for GhPEBE gene. Table S4. Barcode primers for detecting off-target in base editing T0 transgenic plants with deep sequencing for GhCLA gene. Table S5. Summary of genome-wide potential off-targets predictions by Cas-OFFinder tools for target sgRNA1. Table


Additional file 3. Relative risks of COVID-19 diagnosis by Group of Surgery. The complete STATA data are shown for the following: A COVID diagnosis (suspected + probable + confirmed cases). B COVID diagnosis (only probable + confirmed cases).


Supplementary files for the manuscript "Functional outcomes in head and neck cancer patients" submitted to Cancers (Basel) on the 16th of March 2022. Supplemental figure 1. The Head and Neck Carcinoma Functional InTegrity (HNC-FIT) Scales; Supplemental figure 2. Star plot representing percentage (star axes) of HNC-patients with normal or near-normal functional outcome (functional integrity) in 6 functional domains depending on general health status; Supp


Additional file 1: Figure S1. Distribution of major PBM pathogens according to clinical wards in China, 2016–2018. PBM: pediatric bacterial meningitis; Sep, Staphylococcus epidermidis; Eco, Escherichia coli; Spn, Streptococcus pneumoniae; Efa, Enterococcus faecium; Sho, Staphylococcus hominis; GBS, group B Streptococcus; Sha, Staphylococcus haemolyticus; Kpn, Klebsiella pneumoniae; SA, Staphylococcus aureus.


Additional file 1: Table 1. Inpatient ever admission rates within 1 month and 6 months of birth. Table 2. Inpatient ever admission rates by disease (top 5 diseases). Figure 1. Out-of-pocket and reimbursed spending as a share of total spending for inpatient admission. Figure 2. Cost distribution by disease (top 5 diseases). Figure 3. Length of stay by disease (top 5 diseases). Figure 4. Tertiary hospital rate by disease (top 5 diseases).


Figure S3. Fine-mapping and sequence comparison of qGP1(Gn1a): a, the qGP1 was mapped between to Block244; b, the annotated genes inBlock244; and c, the sequence difference of Gn1a between SN265 and R99. (PDF 103 kb)


Additional file 4: Figure S4. No changes in social ability and anxiety of the zebrafish after single or repeated DON treatment. Social ability and anxiety in adult zebrafish were assessed following single or repeated cycles of DON injections. DON (500 ng/g) was administered intraperitoneally for 3 consecutive days (DON). For the repeated episodes of DON injection, a cycle of 3 days of injection was followed by 4 days of recovery, and this cycle was repeated 5 times. A day after the final cycle o


Additional file 1: Supplementary Table 1. List and contents of the detected metabolites in R. glutinosa DDCs and CMCs. Supplementary Table 2. List of differentially accumulated metabolites and their details in R. glutinosa CMCs and DDCs. Supplementary Table 3. Summary of the transcriptome sequencing of R. glutinosa DDCs and CMCs. Supplementary Table 4. Details of the pathways specific differentially expressed genes in R. glutinosa DDCs and CMCs. Supplementary Table 5. Differentially expressed tr


Additional file 34: Fig. S33. Correlation between β-haematin inhibition activity (BIHA50, µM) and anti-malarial activity (IC50-, µM) for quinoline, phenyl benzamides, 4-benzamidopyridine, quinazoline, carbazole, nicotinamide and miscellaneous compounds against sensitive strain C235.


Additional file 1: Supplementary Fig. 1. Estimated marginal means of Log HA titer. This graph shows the Log HA titer levels analyzed by GLM. Glycyrrhiza glabra crude extract (1), Glycyrrhiza glabra methanol fraction (2), Glycyrrhiza glabra chloroform fraction (3), Myrtus communis crude extract (4), Myrtus communis methanol fraction (5), Myrtus communis chloroform fraction (6), Melissa officinalis crude extract (7), Melissa officinalis methanol fraction (8), Melissa officinalis chloroform fractio


Additional file 2: Figure S1. Screening and identification of upregulated circPDHK1 expression in ccRCC tissues and cells. (A) The volcano plot showing circRNAs expression in 4 ccRCC vs. 4 paired adjacent normal tissues from circRNA microarray. (B) Phylogenetic tree (Gene co-expression module analysis). horizontal coordinate: the gene co-expression modules analysis results show that different colors represent different gene modules. Vertical coordinate: the coefficient of difference between gene


Additional file 2. Transcripts with FC > 100 or ≤ 0.01 in RNA-seq analysis. This table shows all the transcripts with FC > 100 or ≤ 0.01 in gut, anal papilla and carcass in BW and FW Ae. aegypti arranged in descending order of FC in each structure.


Additional file 1: Figure S1. Thermogravimetric analysis (TGA) curves of C60 and C60@PDA. Figure S2. DPPH radical scavenging percentage of C60@PDA with different concentrations, and the representative photographs inset. Figure S3. Zeta potential of C60 and C60@PDA. Figure S4. Quantitative analysis of DCF relative fluorescence intensity (n = 4). Figure S5. H&E staining images of main organs, including heart, liver, spleen, lung and kidney on day 7 post-treatment. Mice without any treatment we


Figure S3. Immunocytochemistry for CD86 in primary microglia shows that iron does not exacerbate M1 polarization. Immunolabeling for CD86 (red) did not show a marked increase with addition of FeSO4. No detectable difference in cell number (as qualitatively assessed by DAPI (blue) staining) was noted between groups. Size bar = 50 μm. (TIF 2223 kb)


Additional file 1: Supplementary Table 1. Patient characteristics of the bariatric surgery cohort at baseline (n=27), and where indicated, for serial sample collection timepoints: 3 months post-surgery (n=22) and 6 months post-surgery (n=19). Supplementary Table 2. Prevalence of genus group according to BMI subcategories, diabetes and insulin resistance status in the total population at baseline sampling (n=109). Supplementary Table 3. Sensitivity analyses of the prevalence of each genus group p


Additional file 5: Table S5. The univariate and multivariate analyses of each NIHSS subitems with combined left and right motor symptoms between the posterior LVO and posterior non-LVO group in mild stroke patients.


Additional file 8: Supplementary Video 2. Blood vessel and glioma cells in GBM. 3D rotation and clipping plane of GFAP+ glioma cells (red) in close proximity to a large vessel evidenced by COL-IV (green) and CD31 (magenta). Nuclei are counterstained with DAPI (blue). 3D rendering from Patient 11.


Additional file 1: Table S1. Characteristics of the included population and excluded population. Data are shown as mean ± standard deviation (SD) or median (IQR) for continuous variables and proportions (%) for categorical variables. P values in bold are < 0.05. ACEI, angiotensin converting enzyme inhibitor; AVB, atrioventricular block; BMI, body mass index; CCB, calcium channel blocker; CABG, coronary artery bypass graft; COPD, chronic obstructive pulmonary disease; CTO, chronic total occlus


Additional file 1: Supplementary Table 1. Descriptive characteristics of 306 patients treated with open (n=204) or robotic-assisted partial nephrectomy (n=102) for renal cell carcinoma at a tertiary care center from 01/2003 to 01/2021 following propensity score matching (ratio 2:1); All values are medians (IQR) or frequencies (%).


Additional file 1 A pdf file providing more information on the usage of the proposed framework, in particular the whole Pertussis analysis is showed. Furthermore, a simpler example of analysis is exploited as a step by step guide of the EPIMOD package.


Additional file 2: Figure S1. Geographic distribution per site and per year of a collection of 101 strains of Xanthomonas oryzae pv. oryzae from Pakistan. A collection of 101 strains from two provinces (39 from Punjab and 62 from Khyber Pakhtunkhwa) of Pakistan were used in this study. All the strains were isolated from leaves collected in 2017 (n = 40), 2018 (n = 22), 2019 (n = 24), and 2021 (n = 15). Sampled districts are block shaded, and the number of strains plus collection year is denoted


Supplementary Material 14: Figure S12. The correlation between the flight duration and the stopover duration a. before or b. after each flight event (adjusted flight hours); red dots and line represents dataset from autumn, and green dots and line represents dataset from spring.


Additional file 12. With probes: HCl treatment to mitigate endogenous alkaline phosphatase. Previously unpublished images of 0.4 M HCl exposed for 15 min. Frozen Bovine intestine with Johne disease. A = DAPI; B = Texas Red (Probe is IS 900 Type 1 Red); C = Cy-5 Probe is Bovine β-actin; Type 6 Green) (D = composite of A, B and C. Note scattered apparent “positive “signal in panels “B”, “C” and “D”. Marker bars in µm indicates magnification of × 40.


Additional file 7: Figure S4. Kaplan–Meier analysis of the 11 genes in GSE48075. Kaplan–Meier analysis of TNK1(a), SERPINE2(b), RTKN(c), PRR11(d), MAPK12(e), FABP6(f), ELOVL4(g), DSEL(h), DNM1(i), COMP(j), and C16orf74(k). P-value shows green when p < 0.05. Hazard Radio (HR) shows red when HR > 1 and shows blue when HR < 1.


Additional file 5: Dataset S4. 16S rRNA gene sequencing and quality filtered read counts, SRA database accession numbers, and measures of alpha-diversity metrics (species richness, Shannon, evenness).


Additional file 2. Search strategy for each database. Table 1. CINAHL Search Strategy. Table 2. MEDLINE (Ovid) Search Strategy. Table 3. Embase Search Strategy. Table 4. SocINDEX Search Strategy. Table 5. Scopus (Elsevier) Search Strategy. Table 6. Google Scholar Search Strings.


Additional file 10. Ingenuity Pathways Analysis (IPA) of DEGs specific to the UM, by comparison with the UC-M and UC-F models. (.xlsx). Significantly enriched: (A) Pathway and (B) Disease-related GO terms are shown, with the p-value and the DEGs from which each term was derived. n = 4; data values are in Additional file 5.


Additional file 1: The reliability of LUW thickness measurement. Figure 1. Bland-Altman plot showed the difference of LUWthickness measurement between two operators (Inter-observer reliability)(N=40)]. Figure 2. Bland-Altman plot showed the differenceof LUW thickness measurement between two measurements of the same operator(Intra-observer reliability) (N=46)].


Additional file 1: Additional information on SUSTAIN and PIONEER trials. Figure S1. Ratios to baseline at end-of-treatment for hsCRP with semaglutide and comparators by trial according to baseline tertiles. Figure S2. Ratios to baseline at end-of-treatment for hsCRP by trial according to sex (female/male). Figure S3. Ratios to baseline at end-of-treatment for hsCRP by trial according to statin use (yes/no). Figure S4. Absolute change in hsCRP from baseline to end-of-treatment by trial (semagluti


Additional file 7: Figure S3. Pearson’s correlation test comparing number of cercarial genotypes to the cercarial microsatellite profiles amplified/analysed per Bulinus spp. Pearson’s correlation coefficient, r = 0.04.


Additional file 2: Fig S1. Nuclear and cytosolic R514G-FUS localization in the hippocampus of 12-month-old prnp-FUSR514G mice. (A-C) Confocal images of the CA1 (A), CA3 (B), and DG (C) regions that were co-labeled with endogenous FUS (magenta) or R514G-FUS transgene (red) and nuclear envelope marker, RanGAP1 (green), from non-transgenic and R514G-FUS mice at 12 months of age. Endogenous FUS (magenta) is restricted to nuclei, whereas R514G-FUS transgene (red) showed both nuclear and cytosolic dis


Additional file 2: Figure 2. Conservation and expression analysis of miR-497/195 cluster. A) miR-497 and miR-195 comprise UTRs (yellow blocks) across species. B) & C) Correlation analysis of miR-497 and miR-195 expression from TCGA-CESC datasets and clinical attributes.


Additional file 2: Data S1. Original data for Arabidopsis thaliana. Data S2. The detailed description of features that utilized in this study. Data S3. Gold standard data for Arabidopsis thaliana (AT), Solanum lycopersicum (Solyc) and Zea mays B73 (Zm). Data S4. Values of each gene for genomic features for Arabidopsis thaliana. Data S5. Values of each gene for transcriptomic features for Arabidopsis thaliana. Data S6. Values of each gene for epigenomic features for Arabidopsis thaliana. Data S7.


Additional file 1. Supplementary figure 1. Kaplan–Meier survival curves for patients with category 2 (a, N = 17) and category 4 (b, N = 6) stage IV gastric cancer stratified by the residual tumor status after conversion surgery. * Follow-up time: time from the start of initial chemotherapy to the date of death or of the last follow-up visit Category 2 = distant metastasis that are regarded as technically and oncologically unresectable tumor without peritoneal dissemination. Category 4 = macrosco


Figure S1. Phylogenetic relatedness of the analyzed P. parmentieri strains: A. Genomic Average Nucleotide Identity (gANI) heatmap with dendrograms. B. Gene presence/absence matrix against core pan-genome generated dendrogram. The dendrogram was created basing on hierarchical clustering of the rows. (PNG 1528 kb)


Additional file 1: Figure S1. Percentage of CD24+CD44+CD54+EpCAM+ cells on different days of culture in AGS cells. Cells from the AGS cell line were cultured in non-adherent conditions, without supplemented media, and then harvested for flow cytometry analysis for different cancer stem cell markers on day 0, 1, 3, 5 and 7 of culture. We observed an increase in the CD24+CD44+CD54+EpCAM+ on day 3 compared with other days. Ten thousand cells were acquired by the NXT Attune cytometer. Error bars ind


Figure S8. A) 4T1 tumor weights after single injection of 60 mg/kg gemcitabine administered 17 days after primary tumor implant. B) Flow cytometry analysis of G1, S, and G2/M phase cells in the spleens of mice from (A). C) Flow cytometry analysis of G1, S, and G2/M phase cells in the lungs of mice from (A). Data are mean ± SEM with n = 5–6 mice per group. Significance compared to control (time 0) with *p


Supplementary Figure 1.Relationships of body lengths with measurements of reproductive organs, by maturity stage, for porbeagles (Lamna nasus ) captured from Massachusetts to off the Grand Banks of Newfoundland, Canada, between 2004 and 2018 in this study and for those taken between 1989 and 1999 from Georges Bank to off the Grand Banks of Newfoundland by Jensen et al. (2002).The organ measurements are (A) upper oviduct width and (B) oviducal gland width.


Figure S1. Tpt1, Ciapin1, or Birc6 knockdown by siRNA did not induce cardiomyocyte death.Neonatal rat cardiomyocytes (NRCMs) were transfected with siCtrl, siTpt1 (A, B), siBirc6 (C, D), or siCiapin1 (E, F) at the indicated concentrations (conc.) of 50 nM or 100 nM for 96 h. (A, C, and E) Protein expression of Tpt1 (A), Ciapin1 (C), or Birc6 (E) was evaluated using immunoblot analysis (n = 3). GAPDH was used as an internal control. The left panels show the representative images. The quantified ba


Additional file 1: Pocket Crafter workflow process sample code and example dataset (ChEMBL). SMILES Molecular formula strings. Table S1. Protein Ligand Interaction Fingerprints (PLIF) summary. Table S2. Novartis diverse library HTRF screen result dataset. Table S3. ChEMBL compound set screen HTRF result. Figure S1. Hit CHEMBL1081548 activity confirmation in WDR5 HTRF.


Additional file 1: Figure S1 Timeline of data collection. Figure S2 Flowchart of the study population. Figure S3 Patterns of Biological Rhythm. Table S1 Association between patterns of biological rhythm and life time self-harm behaviour, complete dataset analysis. Table S2 Association between patterns of biological rhythm and recency of self-harm behaviour, complete dataset analysis. Table S3 Association between patterns of biological rhythm and frequency of life-time self-harm behaviour, comple


Supplementary Material 11: Table S11. mtDNA-by-Treatment effect differential expression analysis of the Santiago et al. 2021 dataset. Results of the DESeq2 analysis and the clusterProfiler enrichment analysis of the mtDNA-by-treatment interaction after 4 hours of treatment.


Supplementary files for article Physical activity interventions for the mental health of children: a systematic reviewObjective: This systematic review explored the effectiveness of using physical activity (PA) interventions to enhance psychological well‐being and reduce psychological ill‐being (e.g., anxiety and depression) in children aged six to 11 years old from the general population.Methods: Electronic databases were searched for studies published between Januar


Additional file 6: Table S2. Recombination and chromatin datasets analyzed in this study. This table provides references for each recombination and chromatin dataset analyzed in this study, which contain links to public databases where the data are available.


Supplementary information. PDF containing supplementary methods and tables, pathway impact analysis, heatmap of differentially expressed miRNAs and mRNAs per case study, SePIA pipeline outputs and performance statistics. (PDF 1176 kb)


Figure S2. Normalised read counts for the ten most highly differentiated genes between cultured clinical isolates and laboratory-adapted clones. Individual sample replicates are plotted according to the time of overall peak transcriptome correlation with reference time course data (either 40 or 48 h). Replicates from clinical isolates are in red (those having peak correlation with 40 h are plotted), and replicates from laboratory isolates are in blue. (PDF 312 kb)


Additional file 5. Schematic views of the Excel worksheet for automatic derivation of the componentry of ash samples. a overview, b primary part of the worksheet [inlet in a indicated as], c input and outputby minimization of the RSS of unknown particles by the reference particles of normalized spectra [inlet inindicated as].


Additional file 2: The research material. Table S3. Orphan medicinal products on the market in Finland in May 2024 which had valid orphan designation in May 2022. Table S4. Orphan medicinal products on the market in Finland in May 2024 which had expired orphan designation in May 2022.


Additional file 2: Fig. S2. Specific steps of detecting CD4/CD8 T cells in mouse colonic tissue by FCM. Step 1: Circling lymphocytes: circled the lymphocyte population with FSC and SSC according to the cell size and granularity; Step 2–4: Removing the adhesive: circled the single cells without adhesions according to the fluorescence area and height of laterally scattered light. Then, circled the single cells without adhesions according to the fluorescent area and height of forward scattered ligh


Additional file 1: Supplementary Material Table 1. Bivariate analysis for SRH of Veterans of Reserve Class C, by demographic, physical health, mental health and military service characteristics, LASS 2013. Supplementary Material Table 2 Bivariate analysis for SRH of Veterans of Reserve Class A/B, by demographic, physical health, mental health and military service characteristics, LASS 2013.


Additional file 1: Figure S1. Definition of solvent-exposed pocket grid points. Figure S2. The replica-exchange Monte Carlo protocol used in EDock. Figure S3. An illustrative example of REMC energy trajectories. Figure S4. The spherical coordinate system for generating randomly oriented rotation axis unit vectors. Figure S5. The conformation selection protocol extended from SPICKER. Figure S6. Distribution of ligand size and the number of rotatable bonds. Figure S7. Distribution of the number of


Additional file 3: Table A3. Detailed data of Figure 5 (The provincial distribution of hospitalized patients due to the COVID-19 among the population insured by Iran Health Insurance Organization until March 20, 2021).


Additional file 8 : Fig. 8. Two host states. Panel a, Host inflammatory status (distal colon histology score) distribution over all experimental groups (0% up to 3% DSS). Panel b, Distal colon histology score distributions for groups treated with 1, 2, or 3% DSS, and untreated control group. Colors represent treatment groups as indicated. Bimodal distributions are observed when data from all groups are combined (panel a, Additional Table 6), and within the 2% and 1% DSS groups (panel b).


Supplementary methods: RNA sequencing, shRNA transfection, siRNA transfection, Plasmids transfection, Immunofluorescence, RNA isolation and quantitative reverse transcription-PCR (qRT-PCR), Western blot, Flow cytometry analysis and Soft agar colony formation assays. (PDF 560 kb)


Additional file 1: Figure S1. In order to obtain the limiting current values, we conducted the voltage-sweeping method in our systems. Under the 20nL/min flows was applied near the Nafion membrane, the limiting current value reaches 12 (nA). Figure S2. The V2nd from the measurement has been obtained with the applied current, I. This result showed that the slope of V2nd–I, which is the overlimiting conductance (OLC) by electro-osmotic flows (EOF) have the constant values as 0.21 nS. Figure S3. Th


Supplementary Information files for A gifted SNARC? Directional spatial-numerical associations in gifted children with high-level math skills do not differ from controls<br>The SNARC (Spatial-Numerical Association of Response Codes) efect (i.e., a tendency to associate small/large magnitude numbers with the left/right hand side) is prevalent across the whole lifespan. Because the ability to relate numbers to space has been viewed as a cornerstone in the development of mathematical skills, the re


Additional file 5 Evolution of diaphragm echodensity according to the duration of mechanical ventilation. Thin curves represent the evolution of diaphragm echodensity in each individual. Thick curves with error-bars represent mean and standard error of the mean of the diaphragm echodensity according to the groups.


Supplementary Data required by article entiled "A c-di-GMP signing module controls responses to iron in Pseudomonas aeruginosa"

1) "AF-Q9I243-F1-model_v4.pdb" and "AF-Q9I2P4-F1-model_v4.pdb" are the starting point for IsmP-ImcA complex mdoelling;
2) IsmP-ImcA_Hetero-Tetramer_candidate_model.pdb: IsmP-ImcA complex candidate model;
3) IsmP_predicted_FE_binding_pocket: predicted Fe binding pocket for IsmP.
4）test_86405_relaxed_rank_001_alphafold2_multimer_v3_model_1_seed_000.pdb: pr


Additional file 1. Pediatrics R1 Chlorhexidine-alcohol disinfection. Pediatrics R2 and R4 US and site of insertion. Pediatrics R3 radial vs. femoral artery access. Pediatrics R5 impregnated impregnated CVCs. Pediadrics R6 heparin bonded CVCs. Pediatrics R7 continuous quality improvement program. Pediatrics R8 CHG dressings CVCs and arterial catheters. Adults and pediatrics Prevention R 1-1 and 1-2 subclavian vs. Internal jugular vs. femoral. R 1-3 alc-CHG vs. alc-PVI. R 1-4 1 step vs. 4 steps de


Additional file 1: List of total MAGs and associated antimicrobial, heavy metal, and biocide resistance genes. The excel tables include sample IDs, binned MAGs per sample, taxonomic assignment, and found resistance genes on binned MAGs.


Additional file 1: Figure S1. 21-day Pattern of Mortality – Pre-Christmas versus Summer Low and Christmas Holiday Period. Figure S2. Sex-Specific Risk of Mortality – Pre-Christmas versus Summer Low Period. Figure S3. Sex-Specific Risk of Mortality – Pre-Christmas versus Christmas Holiday Period. Table S1. Sensitivity analyses.


Additional file 5: Fig. A5. Kaplan–Meier plot of overall survival using FGT categories on MRI as strata. 1) almost entirely fat; 2) scattered fibroglandular tissue; 3) heterogeneous fibroglandular tissue; 4) extreme fibroglandular tissue.


Additional file 1: S1. 1H NMR spectra of maximazaphilones I–IV (1–4). S2. 13C NMR spectra of maximazaphilones I–IV (1–4). S3. TheDFT-optimized structures of conformers of maximazaphilones I, II, and IV (1, 2, and 4) with their equilibrium population. S4. Effects of the KRIBB11 (positive control) on cell proliferation and death. S5. Effects of the compounds 6 and 7 at two concentrations (10 μM and 30 μM) on cell proliferation and death. S6. The expression of HSP on HeLa cell treated with 6.


Additional file 2: Figure S1. A forest plot of odds ratios for characteristics associated with prescribing of SGLT-2is in people with type 2 diabetes: complete cases. Figure S2. A forest plot of odds ratios for characteristics associated with prescribing of SGLT-2is in people with type 2 diabetes: imputed dataset. Table S1. Prescribing of SGLT-2is in people with type 2 diabetes; multilevel logistic regression model of imputed dataset.


Supplementary Material 8: Additional file 14: Figure S6. The prediction of TPRX1 derived from Bos taurus isolate L1 Dominette 01449 registration number 42190680 breed Hereford chromosome 18, ARS-UCD1.2, whole genome shotgun sequence. Three possible ORFs for exons, but not introns, are depicted. Putative protein sequence is highlighted in yellow. Sequences from StringTie merge prediction and confirmed cDNA are drawn as lines below the corresponding sequences. Cloning primers are drawn as line arr


Additional file 1: Figure S1. A species-based distribution of BLASTX matches for unigenes from the reference transcriptome of Osmanthus fragrans. We used all the plant proteins in the NCBI NR database to perform the homology search and for each sequence we selected the closest match for analysis.


Figure S1. Location of gene-specific primers and PCR amplification of TaPSTOL genomic regions on DNA extracted from nullisomic (N) / tetrasomic (T) wheat lines. The PCR amplicons correspond to a promoter region (PCR1, 1458Â bp), a promoter and coding region (PCR2, 553Â bp), and a coding region (PCR3, 870Â bp), to demonstrate that TaPSTOL is only present on chromosome 5A. (DOCX 63 kb)


Additional file 1. Figure S1: Whole lung sections. Shown are whole lung sections from SB and different tidal volume ventilated mice. The boxes drawn show areas represented in Fig. 3. Width of box is 450 µm and height is 330 µm


Additional file 2. Additional Table 1: Descriptive information on participating farms regarding the farm structure. Additional Table 2: Descriptive information on participating farms regarding the health status. Additional Table 3: Descriptive information on participating farms regarding the performance. Additional Table 4: Descriptive information on participating farms regarding the clinical signs in weaned piglets


Additional file 1: Table S1. Variability in active compounds of magnolol extracts in various marketed products of ME found in local pharmacy or popular internet sites. Product #7 is the one we called ME.


Additional file 1. Intraclass correlation coefficients and kappa statistics for individual auxiliary nurse midwives vs. dentist with perfect agreement. Validity measures comparing individual auxiliary nurse midwives to the dentist for perfect probing depth agreement.


Supplementary materials [Transparency Checklist]. The related article is Hamann, K. R. S., Wenzel, K., Dasch, S., Junge, E., von Agris, A.-S., & Bleh, J. (2025). How can psychological research support movements for socio-ecological change? A qualitative study on psychological challenges and questions of activists. Global Environmental Psychology, 2025, 3, Article e13089. https://doi.org/10.5964/gep.13089


Figure S4. Reduction of the adipogenic differentiation potential in PreMel. ADSC and PreMel were stimulated for adipogenic differentiation. Red Oil O staining in A) ADSC and B) PreMel. C) Oil Red O+ cells quantification shows that the adipose potential is diminished in PreMel respect to ADSC. Scale bar = 500 μm. *P


Additional file 8: Table S2. Primers designed for the validation of 30 SNPs and the subsequent genotyping of seedless segregants from RxS population and table grape varieties, based on High Resolution Melting analysis (qPCR-HRM).


Supplementary data to support the primary manuscript “Roflumilast foam 0.3% for adolescent and adult patients with seborrheic dermatitis: A randomized, double-blind, vehicle-controlled, phase 3 trial”


Additional file 7: Table S7. Differential orthogroup expression analyses results for comparisons between marine turtle species. Log fold change and adjusted p-values are listed for the green v. leatherback, green v. hawksbill, and leatherback v. hawksbill turtle comparisons.


Additional file 1: Figure S1. Comparison of agreement with A) safety of tap vs. bottled water, and B) statements regarding advice from family and friends about drinking tap water and perception of taste of tap water. Figure S2. Distribution of responses to perception questions.


Additional file 5: Figure S3. Graphic summary of LPC- and LPS-induced distinctive temporal and spatial demyelination patterns. In contrast to LPC-induced robust demyelination with a lag in inflammation/glial response, LPS stimulated strong inflammation/glial response from the early stage and produced diffuse demyelination lesions with its peak demyelination and functional decline later than LPC. OPC/OL, astrocytes, and M/M were scattered throughout the LPS-induced demyelination lesion, but were


Additional file 15: Figure S7. Basic information about the transcriptomic data of flowers. A: expression levels of genes based on RNA-Seq data from the flowers. B: tissue-specific expression of genes in flowers at two opening stages. C: boxplot showing the expression levels of genes from flowers. The raw FPKM data was normalized to Log10. F1: balloon stage, F2: fully open stage.


Additional file 1. Fig. S1. Biomass in the UCPH follow-up experiment. Table S1. Data on soil pH from the HK Kalke experiment and Tables S2â S4. Data on plant elemental composition from the HK Kalke (Table S2), UCPH (Table S3) and CULS (Table S4) experiments.


Additional file 2. Proteomics results. List of proteins, that bind the ALP promoter, identified by mass spectrometry. Nuclear extracts were bound and eluted off the biotinylated DNA on beads and analysed by LCâ MS/MS. Raw mass spectrometry data were analysed in Proteome Discoverer (ThermoScientific; v1.3.0.339) utilising the Mascot database. Samples were searched against Uniprot database to identify proteins bound to the ALP promoter and compared with proteins that nonspecifically bound to uncon


Additional file 3: Table S1. Comparative analyses of orthologous identified in biological pathways. Orthologous in RNAi pathway a, immune responses b, dauer/diapause pathway c and the synthesis, transportation and metabolism of neurotransmitters d were compared among C. elegans, H. avenae and other 6 nematodes with published genomic data. Cel, Caenorhabditis elegans; CCN, Cereal cyst nematode H. avenae; Gpa, Globodera pallida; Mha, Meloidogyne hapla; Min, Meloidogyne incognita; Bxy, Bursaphelenc


Additional file 9 Quantitative validation by significance analysis of DAVID search against other phenotype-oriented resources. We searched the nine relevant genes resulted from the workflow in PheGenI [56], ToppGene [57] and g:Profiler [58]. We quantitatively evaluated this search selecting those terms with a significance less than 0.05 using Benjamini-Hochberg FDR statistic. We obtained a minor result in DAVID search (OMIM search did not offer the phenotypes p-values, unlike GAP DISEASE databas


Additional file 2: Figure S2. KS23 performs no significant effect on the proportions of peripheral Treg subsets. Groups of B10RIII mice were immunized with R161 to induce EAU and treated with PBS, KS23 or TQ23. n = 6 per group. a. Flow cytometric analysis Treg (CD25+Foxp3+) in CD3+CD4+ T cells isolated from the spleens of mice on day 21 after EAU induction. b. The proportion of Treg cells in the splenic cells of mice on day 21 after EAU induction. Results are shown as means ± SD. *p < 0.05; n


Additional file 1: Figure S1. Overview of the metabolic pathways related to succinic acid biosynthesis from glycerol in Y. lipolytica. The conversion of succinic acid to fumarate catalysed by succinate dehydrogenase complex (SDH) should be blocked.


Additional file 4 Table S6. Description of expected MTAs using original SNPs for agronomic traits of Iranian wheat accessions exposed to the well-watered condition. Table S7. Description of expected MTAs using original SNPs for agronomic traits of Iranian wheat accessions exposed to the rain-fed condition. Table S8. Description of expected MTAs using imputed SNPs for agronomic traits of Iranian wheat accessions exposed to the well-watered condition. Table S9. Description of expected MTAs using i


Additional file 8: Figure S5. PU.1 knockdown fails to block most HDACi-induced chromatin accessibility changes in K562. (a) PU.1 expression levels after 72-hour SAHA exposure for vector control-, shRNA 1-, and shRNA 2-transfected K562 cells measured by qPCR. Expression normalized to β-actin. Error bars are SEM (n = 3 replicates). (b) MA plot of fold-change in chromatin accessibility (DNase-seq) between vector control and PU.1 knockdown (shRNA 2) K562 over average signal found at each site. There


Additional file 2. Analysis of in vitro recall proliferation among non CD4 (CD4−) bovine lymphocytes. Recall proliferation of CFSE-labeled CD4− lymphocytes from naïve (A) and CBPP-infected cattle (B) after stimulation with whole inactivated Mmm (T1/44, 5 μg/mL), free Mmm galactan (EPS, 10 μg/mL) or PPDa (10 μg/mL). Different symbols represent the net effect of stimulations (i.e., stimulated cultures minus non-stimulated cultures) for each animal individually (n = 3). Asterisks indicate statistic


Additional file 2. Orangutan sequences and probe tilings present in the human GeneChip microarrays that pertain to pluripotency genes. Alignments of orangutan sequences and selected probe tilings from the human GeneChip arrays pertaining to pluripotency genes listed in reference [39] are provided.


Additional file 1: Table S1. The summary of the Illumina sequencing data. Note: FA: female antennae; MA: male antennae; FH: female head (antennae were cut off); MH: male head (antennae were cut off); FL: female leg; ML: male leg; FW: female wing; MW: male wing; FT: female abdomen-thorax; MT: male abdomen-thorax. Representation of three biological repeats with Arabic numbers 1, 2 and 3. Table S2. Length distribution and quality metrics of C. nigricornis transcripts and unigenes. Table S3. BUSCO a


Additional file 2: Figure S1. Distribution of genes based on COG category of the pan-genome. Distribution of COG categories between the core (blue bars), accessory (green bars) and specific genes (red bars) of Shewanella.


Additional file 13. Gene annotations enriched among ALS-decreased DEGs. (A) Gene Ontology biological processes. (B) Gene Ontology cell components. (C) Gene Ontology molecular functions. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. (E) Reactome pathway database. (F) Disease Ontology. (G) Molecular signatures database (MSigDB). (H) Pathway commons. In (A)–(H), enrichment was evaluated with respect to 666 ALS-decreased DEGs (FC


Additional file 3. Validation of microarray analysis by RT-qPCR. Arabidopsis leaves were infiltrated with STm SL1344 (1 × 108 CFU.mL− 1) or water as a mock control. Expression of randomly selected genes was normalized to the expression of the housekeeping gene ACT8 (AT1G49240). Gene expression levels in the STm 1344-treated samples relative to the mock-treated samples (value set as 1) were calculated using the ΔΔCt method [73]. Results are shown as average (n = 6 ± SE) and statistical difference


Additional file 6. DEGs between the two lines in the TSF flock in response to a tertiary infection. Genes with significantly different abundance between the Resistant and Susceptible lines in response to a Haemonchus contortus tertiary infection in the Trichostrongylus Selection Flock (TSF) at a False Discovery Rate (FDR)â <â 0.05%.


Additional file 9: Figure S7. Expression of wingless (wg) in wild type (A–C) and FoxB knockdown embryos (D–F). In all panels, anterior is to the left, ventral views. Embryos shown in panels A–C and D–F represent different views on the same embryo. Note the reduced/lacking expression in the appendages. Abbreviations as in Fig. 2; hl, head lobe.


Additional file 1: Table S1. Raw numbers of true positives/negatives for both â seedingĘź experiment (Smith College) using N. americanus egg counts and field-sample testing (Smith College and Natural History Museum) for both individual samples and pools.


Additional file 3: Table S1. Comparison of OTU relative abundance between four MFC developmental conditions: early (E), intermediate (I), mature (M) and solution (S), as identified by MiSeq sequencing of 16S rRNA genes. Each developmental stage was represented by three independent biological replicate samples. A nonparametric MANOVA p-value was generated by a comparison between all conditions. As a post hoc test between conditions, each replicate sample was compared against each replicate sample


Additional file 3: Figure S3. Overexpression of the N-terminally tagged Pmp (TVAG_212570) and BspA (TVAG_240680) fusion proteins were tested with regard to their ability to increase the adherence of the T. vaginalis T1 wt strain. Top: examples for every assay are shown, each white or green dot represents one adhering parasite. Below the results of four independent experiments. T-tests were performed for the analysis of statistical significance compared to T. vaginalis wildtype strain T1 (***P


Additional file 4. A ERα binds to the α enhancer in progenitor αT1–1 gonadotropes. ERα binding on the α enhancer chromatin was investigated using ChIP assays in αT1–1 cells. Quantitative PCR was performed using primers targeting the α enhancer genomic sequence. Raw qPCR data were normalized to input. The final results were expressed as fold over the control region. Results are the mean ± SEM of three independent experiments in triplicates. Significant difference with the control region was analy


Additional file 4. Samples cluster by litters at week 3 in PCoA plots. Each dashed ellipse represents the 95% confidence interval for the centroid of each stratification group. Litters were represented in sequential numbers 0â 5. L0 included uninoculated control piglets. Litters 1â 5 included all M. hyopneumonaie experimentally inoculated piglets. Piglets from L3 and L5 showed the least LS whereas those from L2 and L4 showed the highest LS.


Additional file 6: Figure S6. Mettl3 changes the mRNA expression of CBX8. A-B, the mRNA level of Mettl3 and CBX8 were examined after Mettl3 knocked down or overexpression. Data are shown as the mean ± SD of three replicates (*, P < 0.05; **, P < 0.01).


Additional file 1. Questionnaire survey. Specific items included in the questionnaire concerning statements related to brand name drugs, generic drugs and generic substitution, previous experience with drug-related problems of generic drugs and generic substitution, understanding of legal rules for generic substitution in the Czech Republic, and attitudes towards performing generic substitution in pharmacies.


Additional file 1: Table S1. pH and concentrations of Fe, Cu, Zn and Pb in seawater from powdered core samples at different temperature and redox conditions. Figure S1. Photographs of hydrothermal mineral cores for onboard leaching experiment: (a) C9026A 7X-CC (CKL-1), (b) C9027B 1X-CC (CKL-2), (c) C9028A 7S-CC (CKL-3), and (d) C9028A 1H-7 (CKL-4). Figure S2. Images of onboard leaching experiment: (a) an operation in the anaerobic chamber and (b) sample reactions in the water baths. Figure S3. C


Additional file 9: Fig. S6. Principle component analysis. RNA-seq was performed using purified CMs isolated from 8 week old mice. Three independent biological controls (WT, Cre, and fl/fl one of each) and HP1γ KO (Cre;fl/fl) samples were used.


Additional file 10: Table S6. Polymorphic amino acids between B73 and Mo17 alleles of sacd genes. Sacd syntelogs in the Mo17 and B73 genomes were obtained from precomputed resources at MaizeGDB [51, 52] and amino acid polymorphisms were identified via sequence alignments. Specific single amino acid and insertion/deletion polymorphisms between the two alleles are presented.


Additional file 7: Figure S7. Histograms showing estimated optimum process noise values for each component in sub-regions A–G (see Fig. 1) on March 10, 2011. Left and right columns show the TROP and GRAD parameter histograms, respectively, in each region.


Additional file 6: Table S5. a: List of enriched GO terms in ‘GO Biological process’ as obtained by EnrichR using our genes of interest. b: List of enriched ‘KEGG Pathways’ as obtained by EnrichR using our genes of interest. c: List of enriched functions of genes as obtained by ‘GeneMANIA functions’ using our genes of interest.


Additional file 7. The track of the Additional file 6: Reference_Lineage_Movie2.tif contains the xyzt coordinates of the cells, their lineage names and reference ID names. The coloring scheme of the track corresponds to the coloring of bilateral founders in Fig. 5A-A’. The track can be visualized on top of the movie using the Additional file 2.


Additional file 8. Differentially expressed genes between DH lines with extreme phenotypes. gene_id, IWGSC RefSeq v1.0 gene ID; chr, chromosome; LF, mean of normalized counts for DH lines with lower levels of F. graminearum and DON; HF, mean of normalized counts for DH lines with higher levels of F. graminearum and DON; log2FC, log2 fold change; padj, adjusted p-value; description, IWGSC RefSeq v1.0 gene annotation.


Additional file 1: Figure S1. The expression level of BCAR3 in different molecular subtypes. The X-axis represents the 9 molecular subtypes; the Y-axis represents the gene expression. The dotted line represents the average of BCAR3 gene expression levels of all molecular subtypes, including CD1, CD2, CTA, HY, MF, MS, myeloid, NFKB, PR. P = 7.8E−10, Anova test. Figure S2. The expression level of BCAR3 in different ISS stages and 3 serotypes in MM patients. A, The expression of BCAR3 was compared


Additional file 1. Mean gross fatty acid composition of the feed provided during the final seawater stage. The values are calculated on the basis of analyses of the raw materials included in the prescription of the feed (Skretting Norway).


Additional file 4: Table S3. Means of yield related traits of the T4 transgenic families as well as their parental non-transgenic genotype (cv Hi-Line) under field conditions in cultivated seasons 2009/2010.


Additional file 1: Figure S1. Expression of CCR6 in normal adult human testis. Representative western blot results showing the expression of CCR6 in normal adult human testis (nâ =â 5). Î˛-Tubulin was used as loading control.


Additional file 12: Figure S6. Odds ratios and 95% confidence intervals of symptoms and doctor-diagnoses of three allergic diseases for interquartile increases in individual-level annual average concentrations of PM10 (3.80 Î¼g/m3) in 14,614 children at homes stratified by regional and household socioeconomic status from the Seoul Atopy Friendly School Project Survey in Seoul, Korea, for 2010.


Additional file 1: Figure S1. Receiver Operating Characteristic (ROC) curve of the maximum inner diameter of cavities in the prediction of progressive cavity. The area under the ROC curve was 0.74 (95% CI: 0.64–0.84). The optimal threshold for the prediction of progressive cavity was 10 mm, with 73.1% sensitivity and 64.4% specificity.


Additional file 5. Gating strategy for the identification of activated ovine T cell subsets expressing intracellular IL-17A and IFN-γ. Activated ovine PBMC were stained for viability, cell surface markers and intracellular cytokines according to the protocol outlined in “Expression of intracellular IL-17A and IFN-γ by bovine and ovine T cell subsets section” using antibodies listed in Table 3 and acquired using an LSRFortessa™ cell analyzer (Becton Dickenson). Cells were gated to eliminate dead


Additional file 3: Table S3. Geneset Annotations: Worksheets contain pathways enriched among genes upregulated in AD vs. Ctrl (S3a); downregulated in AD vs. Ctrl (S3b); upregulated in AD but not in HuD + FTD vs. Ctrl (S3c); and upregulated in HuD + FTD but not AD vs. Ctrl (S3d). Hypergeometric p-values (p-value), Bonferroni-corrected p-values (E-value), overlaps and input and background set sizes are provided.


Additional file 1. Patient data summary. Patient demographics in summary and in detail at baseline, PCR technical variability, cfDNA data and COSMIC IDs of SNVs. Abbreviations: PSA, prostate-specific antigen; Hgb, hemoglobin; LINE1, long interspersed nuclear elements 1, Mets = metastases, LN = lymph nodes. Offset (h) indicates sample collection time relative to treatment. G-K represent single PCR reactions, L represents pooled sample containing 10 replicate PCR reactions. Patient samples analyze


Additional file 4: Figure S1. Kinetics of Vespula-specific IgE (A) and IgG4 (B) in response to DHS or ALK-AbellĂ˛ VIT (C) Ratio between Vespula-specific IgE and IgG4 during VIT course and follow-up. Data in A and B were analised by ANOVA with Bonferroni post-test (n.s.>0.01).


Additional file 1. Z-ratio analysis of the microarray dataset. (a) Normalized Z-ratio of array intensity data shows a normal distribution of all genes expressed in the STm SL1344-treated leaves as compared to the mock control. The 2% extremes of the bell-shape curve reveal the genes with significant differential expression. (b) Linear regression between relative gene expression calculated with two methods, Z-ratio and Log2 fold change, shows high positive correlation (R2 = 0.9676).


Additional file 2. Experimental infection of urodelan larvae by B. salamandrivorans. Experimental set-up and results from in vivo infection experiments examining the ability of B. salamandrivorans to infect Salamandra salamandra and Discoglossus scovazzi larvae.


Additional file 1: Figure S1. Temperature variations in the culture of A. platensis in industrial-scale open raceway ponds under nitrogen-limited conditions. Figure S2. Gas chromatography analysis of monosaccharide composition of polysaccharide from nitrogen-limited A. platensis. Figure S3. FTIR analysis of polysaccharide from nitrogen-limited A. platensis. Figure S4. 1H NMR and 13C NMR spectrum of polysaccharide from nitrogen-limited A. platensis. Figure S5. Iodine-staining analysis of differen


Additional file 2: Figures S1-S10. showing the odds ratio values split in two categories (between 0-1 and between 1-60) for commonfrequency (MAF > 0.10), intermediate frequency (0.05 <= MAF <= 0.10), low frequency (0.01 <= MAF < 0.05), rare frequency (MAF < 0.01) variants as well as for variants not found in 1000Genomes Project. The PGx variants are colored based on the antipsychotic or antidepressant drug of the association.


Additional file 3: Figure S1. This phylogenetic tree was downloaded from the NCBI website ( https://www.ncbi.nlm.nih.gov/genome/tree/1170 ) which is part of the NCBI C. sakazakii genome tree report resource provided by NCBI and then ST information for each strain in the tree was obtained from the Cronobacter MLST website ( http://pubmlst.org/cronobacter/ ) was manually overlaid onto the tree. The tree/dendrogram in Entrez Genome ( https://www.ncbi.nlm.nih.gov/genome/ ) was developed using pairwi


Additional file 11. A zip archive containing a gallery of each of the cellulases that bound to cellulose in the context of their environment. Each image within the gallery is one snapshot taken from the end of the trajectory showing the relative position of each enzyme (green) that makes contact with the cellulose (red). Nearby lignins are shown in blue, and the substrate tunnel is a yellow surface to orient the viewer. The three tyrosine residues are shown in orange. Note that for each protein,


Additional file 10: Table S15. The 34 up-regulated genes associated with protein yield. The data represent the detailed information of the 34 highly up-regulated genes detected in the liver at 48h post intra-mammary infection (IMI).


Additional file 8: Figure S5. Phylogenetic analysis of selected lipid biosynthesis related genes in H. benghalensis. The protein sequences of H. benghalensis were predicted from PacBio database, andÂ the protein sequences of other species were downloaded from NCBI ( https://www.ncbi.nlm.nih.gov ). Protein sequences were aligned using the ClustalW program, and phylogenetic tree was constructed using the neighbor-joining method in MEGA 5. The scale bar indicates the average number of amino acid su


Additional file 1: Table S1. Mean per cent survival (individual values in brackets) of NF54 after 6 h exposure to 500 nM of DHA, OZ439 or OZ277 using the synchronization protocol from Straimer et al. [44]. Table S2. Mean in vitro IC50 values (single values in brackets) for Plasmodium falciparum isolate Cam3.IR539T and NF54 in the 72-h [3H] hypoxanthine assay.


Additional file 3: Figure S3. ROC analysis for potential biomarkers in predicting outcome of patients with cardiogenic shock receiving ECMO support. ROC curves for plasma biomarkers measured at 0 hr (A) and 24 hr (B) after ECMO support to differentiate the 7-day mortality.


Additional file 2: Figure S1. Phytoliths in Brachypodium distachyon wild type and Bdlsi1-1 mutant. Phytoliths remaining after microwave-assisted acid digestion of plant material (AIR) were recovered from filters, labelled with silica-specific PDMPO dye and observed by light and fluorescence microscopy. Leaves (A, B, C), stems (D, E) and spikelets (F, G) of wild type plants showed high density of silicified structures that varied in the intensity of PDMPO-labelling. In contrast, the leaves (H, I,


Additional file 2. Intact mass analysis of R0.6C. Mass spectra of full-length antigens by LCâ MS and peptide maps from LCâ MS/MS of trypsin digested antigens. (a) Deconvoluted mass spectra of non-reduced R0.6C. (b) Deconvoluted mass spectra of reduced R0.6C. (c)Tryptic peptide maps of R0.6C. In brackets is the calculated accuracy of the measurement to the predicted mass in ppm. All measurements were done in duplicates.


Additional file 1. Complete sequence alignments among mature forms of LipSm and members of its clade. Protein sequences exhibit identities with each other ranging between 85 and 98% (as calculated by Clustal2.1).


Additional file 9. IL1RAPL1 RNA FISH data for the BACs indicated in the cells listed. Xi expression is defined as a single associated with the XIST RNA cloud. Each column represents data from replicate experiments. Therefore, a clone with BAC data in two columns indicates that the experiment was done twice, whereas that in four columns indicates the experiment was done on four occasions


Additional file 1: Table S1. Detectable fold-changes (FC) at 80% power between toxicity groups for the three treatments. Power calculations for comparison of antibody levels between no/mild versus severe toxicity for the three ICI treatments.


Additional file 5: Figure S4. Final survival rate according to the allele origin at pairs of epistatic QTL for resistance to infection with F. psychrophilum. Description: For each figure, abscissa corresponds to the combination of favourable (R) and unfavourable (S) alleles with the grandparent origin in colour (green for B57 and red for AP2) for each pair of epistatic QTL OmyA_OmyB. Survival rates (in ordinate) with similar letters are not significantly different (Fisher exact test P ≤ 0.05 and


Additional file 3: Figure S2. Map of promoter:gene constructs used for sugarcane transformation. SCBV21: Sugarcane bacilliform virus promoter; Ubi1: Maize ubiquitin 1 promoter; Pr4: Ubi1 promoter without heat-shock elements (5′-TGGACCCCTCTCGAGAGTTCCGCTC-3′); E35S: Enhanced Cauliflower mosaic virus (CaMV) 35S (2×35S) promoter; EYFP: enhanced yellow fluorescent protein gene; GUS: β-glucuronidase gene; Nos: Agrobacterium tumefaciens nopaline synthase terminator. Ubi1:GUS (pAHC27 vector) [3] and CaM


Additional file 9: Fig. S7. A model depicting how cyclical switching in CENP-A K124 acetylation and methylation might affect centromeric replication dynamics. A) Model of CENP-A K124me-containing nucleosome. B) Cell cycle progression of K124ac during G1/S to K124me at S phase. C) Current model encompassing the dynamics of how K124 modifications affect replication and kinetochore protein CENP-C binding, before and during replication.


Additional file 3: Figure S2. BD2 Inhibition Downregulates PD-L1 Expression in Prostate Cancer. A. Histograms of PD-L1 expression in PC3 cells treated with RVX208 and/or IFNγ with MFI as indicated, gated on live cells. N = 1/iteration, repeated × 1. B. Summary flow cytometry data for A. N = 1/iteration, repeated × 1.


Additional file 4: Figure S1. Phylogenetic analysis of the Atonal superfamily. A rooted ML tree is shown. All the bHLH families that together constitute the Atonal-related group are indicated. Statistical supports for the nodes that define the different families and the superfamily are indicated: *** (aLRT and aBayes values > 0.95), ** (0.95 > aLRT and aBayes values > 0.8), or * (aLRT and aBayes values


Additional file 7: Figure S4. PU.1 overexpression-driven chromatin accessibility changes are not linked to initial PU.1 ChIP peak strength. (a) PU.1 expression levels measured by RNA-seq FPKM before and after HDACi treatments. Error bars are SEM (n = 2 replicates for SAHA, 3 replicates for NaBut). (b) Fold-change in expression level of PU.1 measured by qPCR following PU.1 overexpression construct transfection and selection. Expression normalized to β-actin. Error bars are SEM (n = 3 replicates).


Additional file 1: Materials and methods. Urine and serum samples preparation before MALDI-TOF/MS analyses; within and between subject variability of serum MALDI-TOF/MS peptidomic features and variability of MALDI-TOF/MS serum peptidomic features; Within- and between-subjects variability of urinary MALDI-TOF/MS peptidomic analysis; Spectra processing; sLOD estimation of MALDI-TOF/MS peptidomic features; Simulation analyses to examine the reliability of ICC for datasets with measurement error and


Additional file 1: Figure S1 . Cellular localization of feline A3s and FIV Vif. HOS cells were transfected with FcaA3Z2b, FcaA3Z3, or FcaA3Z2Z3 (all with HA-tag), together with FIV Vif-TLQAAA. To detect A3 (green) immunofluorescence, staining was performed with an anti-HA antibody. To detect FIV Vif (red) immunofluorescence, staining was performed with an anti-V5 antibody. Nuclei (blue) were visualized by DAPI staining. Figure S2. Comparison of protein sequences of A3s and Vif. (A, B) The sequen


Additional file 3: Figure S2. Evaluation of PIWI antibody cross-reactivity. (A) Alignment of PIWI proteins in the fly Drosophila melanogaster (Dromel), the annelid Pristina leidyi (Prilei), and ascidians: Styela plicata (Stypli), Botrylloides leachi (Botlea), Botryllus primigenus (Botpri), Botryllus schlosseri (Botsch), and Ciona robusta (Ciorob). Conserved PAZ and PIWI domains are shown below the Drosophila melanogaster PIWI sequence (Dromel_piwi_varA). The sequence region used to generate the


Additional file 2: Figure S2. Colonic histopathological changes in C. jejuni infected secondary abiotic Nod2−/− mice. Secondary abiotic wildtype (WT, upper panel) and Nod2−/− mice (lower panel) were generated by broad-spectrum antibiotic treatment and perorally infected with C. jejuni strain 81-176 by gavage at day (d) 0 and d1. Photomicrographs representative for four independent experiments (x100 magnification, scale bar 100 μm) depict histopathological mucosal changes in H&E stained large


Additional file 5: Figure S2. Genomic DNA PCR results and immunoblot results of hydroponically grown ice plants after infection with the A. rhizogenes A8196 or NCPPB 1855 strain harboring the pCAMBIA1303 binary vector. (A) Genomic DNA was isolated from roots of A. rhizogenes A8196- or NCPPB 1855-infected plants infected and uninfected (mock control) plants. Distilled water was a negative control (N) in genomic DNA PCR. The pCAMBIA1303 (1303) plasmid was used as a positive control for the PCR rea


Additional file 3: Figure S3. Images show immunostaining for BrdU and DCX in SVZ cells of Ntg and GET-1 mice 28 days post tMCAO. GET-1 mice did not affect SVZ cell migration in the ischemic brain after tMCAO. Neuroblast migration from the SVZ through the CC to the peri-infarct cortex BrdU+DCX+(neuroblast) double-immunostaining and area quantification in the dorsolateral ventricle (DL) area at day 7 after tMCAO in the contralateral and ipslateral side of Ntg and GET-1 mice. Scale bar=100 Î¼m; Str


Additional file 3: Figure S1. BfmS and functional T6SS are not required for pellicle production. Quantitative evaluation of pellicles formed by: (a) A. baumannii V15 (WT), ΔbfmRS mutant, and ΔbfmRS mutant, complemented with the plasmid pbfmS; (b) WT, Δhcp mutant, and Δhcp mutant, complemented with the plasmid phcp. Pellicle values were normalized by the total growth volume. Error bars represent standard deviation. IPTG denotes induction conditions using 0 or 0.1 mM IPTG concentration.


Additional file 1: Table S1. PAH specific vasodilator therapy cost Table S2. Determinants of above median annual total healthcare cost in SSc-PAH in univariant logistic regression Table S3. Impact of PAH severity on total healthcare cost and its components in SSc-PAH


Additional file 1: Table S1. Primers for site-directed mutagenesis in NFATC1. Table S2. Primers for qRT-PCR. Table S3. Novel NFATC1 phosphorylation sites. Table S4. Phosphorylation-dependent differences in the expression of PIM/NFATC1 target genes in PC-3 cells.


Additional file 1: Figure S1. Nâ€² terminal sequence of each spot of self-marker candidate proteins. Spot no. 3, 4, 8 and 10 were excised from the membrane and sequenced by automated Edman degradation at the Instrumental Analysis Division, Creative Research Institution of Hokkaido University.


Additional file 1: Figure S1. Serial changes in cerebral rSO2 (non-representative cases). Serial changes in cerebral rSO2 (non-representative cases). (A) 74-year-old man; patient #2. His initial electrocardiogram was ventricular fibrillation, and the values of cerebral rSO2 gradually decreased after start of measurement. ROSC was diagnosed in the hospital setting after application of ECMO. Her outcome at discharge was Dead. (B) 45-year-old woman; patient #5. This patient showed the low rSO2 type


Additional file 1. Supplementary data describing substructural analysis of extracted ChEMBL compounds, statistical analysis of enriched target prediction of RECAP compounds, separation in medians of active/inactive docking score distributions for the docking models, computed logP and tPSA values and selectivity profiling data for compounds 1–25, docking parameters used, scripts for compound extraction from the ChEMBL database, computation of Mann–Whitney test and F1 scores.


Additional file 2 Figure S1. Network of the top-50 predicted associations for iBD and asthma obtained by our method. ellipses with Orange and circles represent diseases and microbes, respectively. the blue lines and red lines denote the associations of predicted microbes with iBD and asthma, respectively.


Additional file 1: The microbial richness and diversity in patients with PNS before initial therapy. Microbial richness was reflected by the observed OTUs, Chao1 and ACE index, while microbial diversity was indicated by the Shannon, Simpson, InvSimpson and Coverage index. No significant differences were found in these indices among these three groups (formula feeding, breasting feeding, formula and breast feeding group) before initial therapy (p > 0.05).


Additional file 5. sQTL results. Sheet1. All sQTL results in both conditions (Control and Treated). Sheet2. Significant sQTL results in both conditions, along with predicted coding consequence, location within transcript, and matches to the ESE and ISE. Reference list for ESE and ISI.


Additional file 2. Sequence of the guide RNA constructs that were synthesized. Colors of the nucleotides infer different purposes. Blue, primer binding sites for amplification and cloning into plasmids; grey, ribozymes; red, 20 nucleotides specific to hos1; purple, guide RNA; black, stop codon for trp3. Underlined nucleotides will base pair in the hammerhead ribozyme. Bold is the XhoI restriction enzyme site.


Additional file 12: Figure S12. Maximum-Likelihood phylogenetic tree of trypanosomatid amastins. The tree was inferred using IQ-TREE v.1.5.3 with the JTT + I + G4 model and 1000 bootstrap replicates. The support values are in the following format: SH-aLRT support (%)/bootstrap support (%).


Additional file 1: Figure S1. Schematic representation of the hBM-MSC-sEV isolation processing workflow. A 7-day timeline was designed for hBM-MSC-sEV production where the cell-conditioned medium was collected and processed for EV isolation as described.


Additional file 4: Figure S3. Lower and upper confidence intervals for all parameters (dilution, variance of DGE, variance of IGE, and genetic correlation between direct and indirect effects) for different group sizes (schemes) with different CV and $${\bar{\text{n}}} = 6$$ n ÂŻ = 6 . The black horizontal lines show the true simulated values and the black dots show the estimates. The group compositions are random.


Additional file 2: Figure S2. Comparison of the two genotyping platforms. DSP (electropherograms) and ASO (amplification plot) detected the presence of four common genetic variations in the CYP2D6 gene including C100T, G1846A, C2850T and G4180C. The X axis of the amplification plot shows the relative fluorescence for wild-type alleles (green curve) and variant alleles (blue curve), respectively. Clusters of the homozygous wild-type, heterozygous and homozygous mutant are also shown in the alleli


Additional file 1: Figure S1. Immunostaining negative control for 2Â week IOP elevated retina sections. No primary antibody for endothelin A (ETA) receptor or Î˛-III-tubulin (TubIII) was added. DIC image show retinal layers.


Additional file 11: Fig. S7. (A) Quantification of cardiac cross section area after TAC. More than 100 CM cross sections per animal were analyzed. Two-way ANOVA followed by multiple comparison was perform. TAC operation increased cross section area significantly; however, no interaction with genotype was detected. No significant difference was detected between genotype in Sham or TAC mice. (B) Quantification of cycling nuclear number in the heart. Heart sections were stained with Ki67 antibody a


Additional file 4: Table S2. Datasets of the nematoda ESTs used in this study. The datasets from NCBI and Nembase 4 databases consist of 1,331,401 ESTs sequences from 45 genuses and 75 species clustered into three life styles. * Life style classification: FLN, free living nematode; PPN, plant parasitic nematode; APN, animal parasitic nematode.


Additional file 2: Table S1. Summary of measured and calculated variables at each speed. Pmech; mechanical power output for vertical directions, ΔEE; differences of energy expenditure between uphill and level gradients or between level and downhill gradients, MRvert; metabolic rate for vertical directions, and Effvert; vertical efficiency. Values are mean (±SD).


Additional file 2. Fig. S1. Wild-type and transformed roots of chickpea cultivar Annigeri grown in selection medium. Fig. S2. Green fluorescent protein (GFP) visualization by confocal microscopy in transformed chickpea (cultivar Annigeri) roots. Fig. S3. Characterization of transformed roots in chickpea cultivar Annigeri. Fig. S4. Green fluorescent protein (GFP) expression in different chickpea cultivars. Fig. S5. PCR analysis of transgenic chickpea roots expressing GFP. Fig. S6. Characterizatio


Additional file 3. The raw chemical data (total ion count) per sample for Atta colonies. Peaks are numbered according to Table 1 and additional file 1; sample names include information about colony identity (first word) and whether the sample is a cockroach (“roach”) or a worker (“mw”).


Additional file 3: Table S3 Chromatin states in monocytes and macrophages. Chromatin states in both monocytes and macrophages defined using a hidden Markov model based on H3K4me1, H3K4me3, H3K27ac and H3K27me3 histone marks available from public BLUEPRINT data.


Additional file 1. “IDI Guides Ibis 9.13.16.doc” Title: Innovative Incentive Strategies for Sustainable HIV Testing and Linkage to Care: In-Depth Semi-Structured Interview (IDI) Guides. Description: The document contains two in depth semi-structured interview guides used in the Innovative Incentive Strategies for Sustainable HIV Testing and Linkage to Care (Ibis) study.


Additional file 4: Figure S4. Colony formation shown in Fig. 3 separated by erythroid and G/M colonies. (A) Erythroid and (B) G/M colony formation from MPN patients and normal controls with increasing concentrations of LCL-161. (C) Erythroid and (D) G/M colony formation from MPN patients and normal controls with 10 ng/ml TNFα + increasing concentrations of LCL-161.


Additional file 9: Figure S5. KLK9 free monomer detection by the newly developed KLK9 ELISA upon the formation of serpinA3–KLK9 heterocomplexes. Mat-KLK9 (0.5 μg) was incubated either alone (control) or with different amounts of the human recombinant serpinA3 inhibitor (R&D systems) [at molar ratios (KLK9/SerpinA3): 1/0.2, 1/0.5, 1/1 and 1/2] in 50 mM Tris–HCl (pH 8.0) for 1 h at 37 °C. The samples were further diluted with 6% BSA and the assay was performed by following the described protoc


Additional file 8: Figure S8. Regulation of genes of Long term depression pathway during senescence induction in HFF strains. Genes of the “Long term depression” pathway which are significantly up- (green) and down- (red) regulated (log2 fold change >1) during irradiation induced senescence (120 h after 20 Gy irradiation) in HFF strains. Orange color signifies genes which are commonly up- regulated during both, irradiation induced and replicative senescence.


Additional file 7. Sexual dimorphism. â Photograph taken in forest in French Guiana. The contrasted shape and size between a female and male spiders (Araneidae) on her web.â Attribution: MaĂŻlis Huguin (Institut Pasteur de la Guyane, French Guiana).


Additional file 5. The overlap of differentially expressed genes in inflorescences between six pairs in Jatropha. The pairwise ckII_ckI, dxhII_dxhI, dxhI_ckI, dxhII_ckII, gII_ckII and gII_dxhII indicate the same pairs shown in Fig. 3. All differentially expressed transcripts in Additional file 5 were listed in Additional file 4.


Additional file 5: Figure S5. Maximum likelihood tree of SND1 and related proteins. The protein sequences of OG0009898 (red) and OG0001875 (green) are obtained from van Velzen et al. (2018). Species include arabidopsis (Athaliana), Eucalyptus grandis (Egrandis), Fragaria vesca (Fvesca), Glycine max (Gmax), medicago (Mtruncatula), Populus trichocarpa (Ptrichocarpa), Parasponia andersonii (Pan) and Trema orientalis (Tor). Numbers at to the branches indicate support from 1000 ultrafast bootstrap re


Additional file 6: Figure S3. PU.1 and H3K4me1 immunoprecipitation. ChIP-qPCR for six DHS sites that open in K562 cells following HDACi treatment and a control site that does not change accessibility. PU.1 enrichment before and after 72-hour (a) SAHA or (b) NaBut treatment and (c) H3K4me1 enrichment before and after SAHA treatment for each site was measured. Enrichment is normalized to that measured in respective input control samples. Error bars are SEM (nÂ =Â 3 replicates).


Additional file 2: Table S1. Correlation matrices of song parameters from song thrush males recorded in urban forests and natural forests. Significant values are indicated in bold. Table S2. Correlation matrices of song parameters from song thrush males recorded in urban forests. Significant values are indicated in bold. Table S3. Correlation matrices of song parameters from song thrush males recorded in natural forests. Significant values are indicated in bold. Table S4. Results of the best fit


Additional file 2: Figure S1. Expression levels of has-miR-451a, has-miR-150-5p, has-miR-27b-3p and has-miR-92a-3p using purified RNA as template with the S-Poly(T) Plus method . Each volume of plasma and corresponding serum were from a same healthy donor. miRNA levels were normalized to spiked-in cel-miR-54-5p. Data are shown as means ± SE, ***p < 0.001.


Additional file 4: Table S3. Treatment failure of Stenotrophomonas maltophilia hospital-acquired pneumonia. Description of treatment failures of Stenotrophomonas maltophilia hospital-acquired pneumonia.


Additional file 8: Figure S3. Poisson's law distribution during encapsulation process. The number of yeast cells per droplets (k) after the encapsulation process is following a Poisson distribution and depends on the initial average number of cells per droplets (λ) of the encapsulated cell suspension.


Additional file 1: Figure S1. A typical elution profile of the recombinant keratinase by gel filtration chromatography column. Peak 1: unknown protein; Peak 2: BsKER71 protein; Red peak: ammonium sulfate.


Additional file 1: Appendix. Outline of model-fitting for 17 March 2015 magnetic cloud. Table A1. Results of fitting with a cylinder model. Table A2. Results of fitting with a torus model. Figure A1. Drawings of seven torus geometries listed in Table A2 projected onto the YZ plane.


Additional file 3: Figure S3. Second derivatives of the FTIR absorption spectra of S. cerevisiae BY4741 and opi1Δ cells, in the absence and in the presence of lactic acid: cell wall carbohydrate absorption between 1200 - 900 cm−1. Cells were grown in shake flasks in minimal (YNB) medium with 2 % w/v glucose in the absence (a, b) and in the presence of 46 g/L of lactic acid (LA) (c, d) at pH3. FTIR analysis was performed at 18 h (a, c) and at 40 h (b, d) after the inoculation, corresponding to th


Additional file 3: Figure S1. Immunophenotypic analysis for ZJU-0430 as determined by flow cytometry. The representative graph of CD24, CD44, CD29, CD34, CD90, CD117, CD133, CD184 CD326, CD338 protein expression were detected on primary cells and P100 cells of ZJU-0430.


Additional file 6. Frequencies of cytokine-producing lymphocyte subsets for all investigated organs and stimulation variants. Frequencies of cytokine-producing lymphocyte subsets for all investigated organs and stimulation variants are given in this table. In addition, all calculated E. coli corrected values for control and infected birds are listed.


Additional file 2: Fig. S2. Heat map of ratios of transcript abundances between tobacco organs in a log2 scale. Transcript abundances in each tissue were taken as reference which can be recognized as a blank column. (a) Ratios with young leaves as control, (b) Ratios with fully expanded leaves as control, (c) Ratios with roots as a control, (d) Ratios with flowers as a control, (e) Ratios with floral buds as a control. A log2 (ratio) > 0 is represented by blue colors and a log2 (ratio) < 0


Additional file 1. Relation between expression efficiency and DNA/Gold particle concentration. Graphs of the average number of fluorescent cells in a 10Â Ă image area after bombardments with different gold particle concentrations and plasmid DNA concentrations.


Additional file 2: Figure S2. Time course studies to determine biomass, total lipid yield and lipid content of MNNG treated mutants. Mutants (a) YlC4, (b) YlC5 and (c) YlC6 were grown on 100 gL−1 WCO as mentioned in Methods. All values are represented as mean ± SD, determined after three independent experiments. Biomass (gL−1) -black down pointing triangle,Total lipid yield (gL−1) -black circle, Lipid content (%)-○. Inset: In each graph, light microscopy (left panel) and Nile red fluorescence mi


Additional file 2: Figure S2. Column purifications for density aggregation activity. Mono Q and phenyl sepharose fractionations of conditioned media from WT for density-dependent aggregation activity (see Fig. 2a). Protein profiles are indicated, with relative salt concentration elutions.


Additional file 1.Â Figure S1: Locations of stations. Table S1: Summary of JAMSTEC OBSs (BBOBSs and SPOBSs). Table S2: Summary of ERI OBSs (LTOBSs). Figure S2: Histogram of the magnitudes for local earthquakes. Figure S3: Example of seismic record for a local earthquake. Figure S4: Ray paths of seismic phases isolated from teleseismic P wave for seafloor observation. Figure S5: Hypocenters determined by tomoDD. Additional Note 1: Time-domain RFs. Figure S6: Examples of time-domain RFs. Figure S7


Additional file 1. Mean, median and standard deviation (SD) values of panicle-related traits in O. barthii (B88) and O. glaberrima (CG14). PB primary branch, Sb secondary branch, Sp spikelet. For each species, nÂ =Â 18.


Additional file 2: Table S7. BUSCO analysis of KitaakeX and comparison with other rice genomes. Table S8. Summary of transposable elements in KitaakeX, Nipponbare, and Zhenshan97. Table S9. Comparison of SNPs and INDELs between three rice genomes. Table S10. Comparison of single base substitutions between three rice genomes. Table S11. Oryza sativa KitaakeX annotation v3.1 on assembly v3.0. Table S12. Sequence length of pseudomolecules, number of genes and gene models for each of the 12 rice chr


Additional file 5. A Knockdown efficiency of ERα SiRNA in αT3–1 cells. αT3–1 cells were transiently transfected in duplicates with scramble or Erα SiRNA. Proteins were extracted 48 h later. Western blots for ERα and GAPDH immunodetection were performed as indicated in “Materials and Methods.” Top: ERα immunodetection: The 66-kDa and the 36-kDa isoforms are expressed in αT3–1 cells. Erα SiRNA allows efficient knockdown of both isoforms. Bottom: GAPDH immunodetection for normalization. B ERα speci


Additional file 2. Formulas to synthesize survey proportions into weighted aggregated estimates. Five different methods and formulas used to calculated weighted averages of survey proportions of people who self-identified as lesbian, gay or bisexual.


Additional file 6: Figure S5. Chemical screen results and validation. A. Box plot of all raw luciferase measurements from the chemical screen by individual 384-well plate, demonstrating lack of obvious batch effect. Chemical library plates were prepared and assayed as one batch of 15 plates. B. Chart displaying results from the Xi-luciferase assay in the 24-well format upon treatment with various chemicals (at 10 μM) in the presence of 5-aza-2’-dC (0.2 μM) for 72 hours. Error bars indicate one s


Additional file 1: Table S1. Generic and species-specific primers used for the detection of haemoparasites in woylies. Table S2. Molecular methods for piroplasm PCR and Sanger sequencing/phylogenetic analyses of haemoparasites. Table S3. Number of woylies sampled within Dryandra, with prevalence of infection (and Jeffreyâ s 95% CI). Table S4. Number of woylies sampled within Walcott, with prevalence of infection (and Jeffreyâ s 95% CI). Table S5. Number of woylies sampled within Warrup East, wit


Additional file 4: Figure S4. Confocal image of bacteria flowing inside microchannel. The fluorescent tagged spiked bacteria in PBS was introduced into PEO based elasto-inertial flow, and no displacement of bacteria is observed close to the outlet in elasto inertial flow, presumably due to the insignificant magnitude of forces on bacteria.


Additional file 4: Figure S3. Analysis of pedigree records of 27 animals with dwarfism. Description: Pedigree of 21 animals with dwarfism (a). The pedigree contains only obligate mutation carriers. Red and yellow colours indicate 21 affected animals and 14 genotyped haplotype carriers, respectively. Green and blue colours indicate the sire of 21 affected animals and DWhet, respectively. Rectangles and ovals represent male and female animals, respectively. Pedigree completeness of another six ani


Additional file 6: Figure S5. In vitro splicing assay of different Arabidopsis [32P] labeled pre-mRNA substrates. Radiolabelled AP2M, TIP41 and PTB1 pre-mRNA substrates were incubated individually at 30 °C in the Arabidopsis nuclear extract as described previously. Samples were withdrawn at indicated times, extracted [32P]-RNA and analyzed by electrophoresis on a 6% polyacrylamide-7 M urea gel, followed by autoradiography. The sizes of the pre-mRNA substrates are indicated at right.


Additional file 2: Figure S1. The absence of hydrophobins has minor influence on hydrolytic enzyme transcription. The transcription of cbhA and xlnA during SSF of SEB was moderately increased in the individual absence of RodA or DewC.


Additional file 5: Table S4. Progress of annual influenza vaccination program from October 1 through December 30, year 2007 and 2008 respectively. The year 2007 influenza vaccination program started on October 1 and aimed at those aged 6 months through 2 years, the school children aged 6-7, and the elderly aged 65 years and above. From December 1 on, the program was open to the general population to maximize the vaccine utilization. As to the year 2008 influenza vaccination program, starting on


Additional file 9: Figure S9. (a) FACS analysis was employed to sort CD68+ macrophages from LLC tumor and the VEGFA level in CD68+ macrophage was measured. SIRPα-Fc enhanced macrophage infiltration without significant VEFGA production in the tumors. (b) CD11c was used as a marker to detect dendritic cells in LLC tumor treated with SIRPα-Fc or VEGFR1-SIRPα.


Additional file 3: Figure S3. Heatmap analysis of DEGs related to plant hormone signal transduction. The expressions of gene are displayed different colors. Red means high expression, and blue means low expression.


Additional file 3. The UPRIGHT Consortium. The map of the Consortium and the list of institutions participating in the UPRIGHT project is provided in this file. List of the researchers included in the â on behalf of the UPRIGHT Consortiumâ term is also provided.


Additional file 4. Sequence polymorphisms in γ-TMT2 gene and promoter region. A. γ-TMT2 gene structure is depicted as follows. Exons are shown as red boxes, and areas between two exons are introns. The 5′-UTR and 3′-UTR are shown as grey boxes. Arrows shows all SNPs and indels located in cis-elements (C). Numbers above arrows correspond to DNA polymorphism numbers in (B). B. All SNPs and indels found in gene and promoter region between TK780 and B04009. Third row shows the position of nucleotide


Additional file 6: Figure S4. Fold changes for efflux genes following exposure to either chlortetracycline at 32 Âľg/ml or florfenicol at 16 Âľg/ml for 30 min. Numbers in bold indicate significantly differentially expressed genes (FDR < 0.05). Genes that are up-regulated are colored blue while down-regulated genes are in red; the intensity of the color indicates greater fold change.


Additional file 1: Figure S1. Nanoaspiration method development. (A) Fluorescein-tagged-BSA being aspirated and released on top of APTES-coated coverslips. Fluorescence of BSA solutions can be tracked inside the nanoaspirator and on the coverslip, when imaged under the FITC filter channel. (B) Real-time current measurements were used to track the nanopipette’s entry into the cytoplasm and nucleus of cells. Nuclear entry resulted in a greater magnitude of ΔA (i.e. change in current). (C) GFP-enco


Additional file 1: Figure S1. FNRG mice are highly engrafted with primary human hepatocytes. FNRG mice were transplanted with cryopreserved primary human hepatocytes. Mice were bled every few weeks, serum was isolated, and a human albumin ELISA was run to assess engraftment levels.


Additional file 3 Fig. S1. Climate condition include precipitation and temperature of field trail site during the 2016–17 and 2017–18 cropping seasons. Fig. S2. The pattern of LD decay in different chromosomes of genome A in T. aestivum based on original SNPs. Fig. S3. The pattern of LD decay in different chromosomes of genome B in T. aestivum based on original SNPs. Fig. S4. The pattern of LD decay in different chromosomes of genome D in T. aestivum based on original SNPs.


Additional file 1. Principal component analysis score and loading plots of the first 5 principal components from NIR data of undyed unaged wool, undyed light aged wool (600 h, 150,000 lx) and historic fragment wool yarns. Outlier points have been highlighted in red.


Additional file 1: Figure S1. (a) SEM image of CNT, (b) SEM of CNT@SiO2. Figure S2. Thermogravimetry of CNT@Li2FeSiO4 and CNT@Li2FeSiO4@C. Figure S3. The dQ/dV vs. voltage plots of CNT@Li2FeSiO4@C at 0.2 C. Figure S4. The Nyquist plot of CNT@Li2FeSiO4 and CNT@Li2FeSiO4@C electrode and the equivalent circuit for electrodes.


Additional file 3: Table S3. Spearman rank correlations matrix. Spearman correlations between all environmental variables measured in the 26 mosquito larval habitats, climate variables extracted from WorldClim v.2 and MODIS vegetation indices. Variables: pH; WT, water temperature; conductivity; TDS, total dissolved solid; DO, dissolved oxygen; salinity; alkalinity; APC, aquatic plant cover; PRS, soluble reactive phosphorous; NO 3 - +NO2+, nitrate+nitrite; NH4+, ammonia; WD, water depth; area; Ai


Additional file 2. Mapping statistics of input read pairs mapped to the reference genomes. Sequencing reads derived from the Chinese strain SRA data were mapped to the Treponema pallidum subsp. pallidum (TPA) SS14 and Nichols reference genomes [6] and to the rabbit genome (Statistics was calculated from post-processed mappings; repetitive and homologous sequences and PCR duplicated reads were excluded from the statistics).


Additional file 7. Levels of IgM and IgE measured to the 40-multiplex panel in the WHO reference reagent and IgM pool compared to negative control and blanks under two different incubation conditions. Incubation conditions compared are: 4 °C (4 °C ON) vs 2 h at 37 °C (37 °C 2 h). A) Predicted 5PL curves of IgM levels in the IgM pool. B) Predicted 5PL curves of IgM levels in the WHO reference reagent. C) IgE levels in the WHO reference reagent.


Additional file 2. Flow cytometry of protoplasts co-transformed with pUbi-Cas9-rbcS and pUbi-YFP-rbcS. Protoplasts were diluted to different concentrations (50,000–500,000 cells per 200 μL) for transformation, and either incubated with the DNA for 10 min prior to the addition of PEG or not incubated with the DNA (PEG added immediately to DNA/protoplast mixture). The percent of protoplasts expressing YFP is indicated. MFI, mean fluorescence intensity of YFP-expressing protoplasts.


Additional file 1: Figure S1. Genes engaged in respiratory mechanism of Burkholderia sp. ISTR5. Figure S2. Transcriptional regulators involved in the regulation of various functions in Burkholderia sp. ISTR5. Figure S3. Transporters responsible for the movement of substrates and ions in Burkholderia sp. ISTR5. Figure S4. Oxidoreductases responsible aromatic compounds degradation found in Burkholderia sp. ISTR5. Figure S5. Representation of various stress regulation proteins found in Burkholderia


Additional file 1: Table S1. Volumetric PHB production, DCW, and PHB content of recombinant R. sphaeroides HJ strains. Table S2. Volumetric PHB production, DCW, and PHB content of recombinant R. sphaeroides HJ strains in various concentration of AS. Table S3 PHB synthetic genes in R. sphaeroides 2.4.1 and its corresponding genes in R. capsulatus SB 1003 strain. Table S4 Primers used in this study.


Additional file 13. List of 60 misbehaviours with alternative formulations and newly added items from the focus group interviews printed in italics. Newly added and preferred formulations of existing items are printed italic-bold and the former formulation is crossed out.


Additional file 1: Table S1. Fault model parameters of SSEs in southwest Japan estimated by Nishimura et al. (2013). Figure S1. Calculated tilt change for all fault models. Red arrows indicate the calculated tilt change due to a uniform slip of a rectangular fault shown by a blue rectangle in each panel, in which the slip vector is indicated by a blue arrow.


Additional file 5. Specificity of the ΔxlnR response patterns on xylose, xylan and straw. Substrate-dependent scatter plots of log2 (FC) values in the ΔxlnR strain as a function of log2 (FC) values in the wild type strain. The GOIs are gathered according to the five gene clusters that have been defined in Fig. 2.


Additional file 1. Additional figures. Figure S1. Growth inhibition by expression of the site-mutants of Pdr transcription factors, and determination of suitable galactose concentrations. Figure S2. Determination of alkane exposure concentrations. Figure S3. Growth of BYL13 expressing Pdr transcription factors in the presence of alkanes. Figure S4. Western blotting of Pdr proteins in BYL13. Figure S5. Tolerance of BY4741 expressing Pdr transcription factors against C10 and C11 alkanes.


Additional file 8: Figure S8. Production of pellet and extracellular proteins by â pyrF::CaBglA with (pH 5.5) or without (Untreated) pH value adjustment at the cellulose hydrolysis stage. No dramatic change was detected during the whole saccharification process. But lower amounts of pellet and extracellular proteins were produced under pH 5.5 condition compared to the untreated control, indicating the blocked cell growth and the reduced expression of extracellular protein, including cellulosomal


Additional file 1 Supplementary information. Detailed methodology for fractal Monte Carlo sampling and Bayesian deconvolution of Hi-C specific contacts. Also, additional figures referenced for this study.


Additional file 2: Figure S2. Example Co-expression Between IAPs. BIRC5 mostly showed anti-correlation with NAIP expression across different cancers (A). Between BIRC5 and BIRC7, both positive and negative correlations were observed across the 32 cancers (B).


Additional file 2: Figure S2. Kaplan-Meier curves of OS (A) and DSS (B) for the SEER cohort between patients with IA unchanged and those with anatomical stage IA upstaged to prognostic stage IB; Kaplan-Meier curves of OS (C) and DSS (D) for the SEER cohort between patients with IIB unchanged and those with anatomical stage IIB upstaged to prognostic stage IIIA.


Additional file 11: Figure S10. Schematic of Alu-gag two-step nested qPCR. First PCR to amplify Alu-proviral DNA junction was performed using PCR primers targeting the Alu (black arrow) and gag (pink arrows). Red wavy line fused to gag-primer indicates the tag-sequence for 2nd qPCR primer binding. In second qPCR, viral DNA fragments were amplified by LTR primer (blue arrow) and tag-primer (red arrow). The green box indicates the position of the TaqMan probe for gag.


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Journal Article BOOK REVIEWS Get access The Penguin Book of Comics. By GEORGE PERRY and ALAN ALDRIDGE Penguin Books. 1967. pp. 256. 25s. RAYMOND DURGNAT RAYMOND DURGNAT St. Martin's School of Art Search for other works by this author on: Oxford Academic Google Scholar The British Journal of Aesthetics, Volume 8, Issue 3, July 1968, Pages 309–310, https://doi.org/10.1093/bjaesthetics/8.3.309 Published: 01 July 1968


Journal Article The United Nations and the maintenance of international peace and security Get access The United Nations and the maintenance of international peace and security. By N. D. White. Manchester: Manchester University Press. 1990. 240pp. Index. £35.00; ISBN 0 7190 3227 X. John Main John Main 1University of St Andrews Search for other works by this author on: Oxford Academic Google Scholar International Affairs, Volume 67, Issue 3, July 1991, Page 571, https://doi.org/10.2307/2621961 Pu


Journal Article Journal of Jasper Danckaerts, 1679–1680. Edited by Bartlett Burleigh James, B.D., Ph.D., and J. Franklin Jameson, Ph.D., LL.D. [Original Narratives of Early American History.] (New York: Charles Scribner's Sons. 1913. Pp. xxxi, 313; two maps and one view in facsimile.) Get access Journal of Jasper Danckaerts, 1679–1680. Edited by James Bartlett Burleigh, B.D., Ph.D., and Jameson J. Franklin, Ph.D., LL.D. [Original Narratives of Early American History.] (New York: Charles Scribner