{ "immunotechnology_R2_pos_000599": { "query": "Find immunoblotting experiments detecting XIAP in cell lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "XIAP" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002982": { "query": "Find immunoblotting experiments detecting JAG1 in cell lysate with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "JAG1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002192": { "query": "Find immunoblotting experiments detecting IL-1β in total protein extract with 40 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg/lane", "parsed": { "kind": "point", "number": 40.0, "unit": "ug/lane", "raw": "40 μg/lane" } }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R2_pos_000503": { "query": "Find immunoblotting experiments detecting p65 in tissue homogenates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "p65" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": null }, "immunotechnology_R1_pos_000792": { "query": "Find immunoblotting experiments detecting ERK1/2 with membranes blocked in 3% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "3%", "parsed": { "kind": "point", "number": 3.0, "unit": "%", "raw": "3%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1/2" } ], "explore_field": null }, "immunotechnology_R1_pos_002448": { "query": "Find immunoblotting experiments detecting p53 in tissue lysates with a loading amount of 30 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "p53" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000011": { "query": "Find immunoblotting experiments detecting SHP2 in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "SHP2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000003": { "query": "Find immunoblotting experiments detecting MMP-2 in whole cell lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "MMP-2" }, { "field": "sample_type", "op": "=", "value": "whole cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002090": { "query": "Find immunoblotting experiments detecting RhoA in cell lysate with a loading amount of 40 μg.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "RhoA" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000883": { "query": "Find immunoblotting experiments detecting HIST3H3 in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "HIST3H3" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_000276": { "query": "Find immunoblotting experiments detecting AKT with membranes blocked in 5% low-fat milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "Low-Fat Milk" }, { "field": "protein_targets.target", "op": "=", "value": "AKT" } ], "explore_field": null }, "immunotechnology_R2_pos_000116": { "query": "Find immunoblotting experiments detecting SRSF1 in cell lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "SRSF1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000786": { "query": "Find immunoblotting experiments detecting PKM2 in total protein extracts prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PKM2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_002429": { "query": "Find immunoblotting experiments detecting MMP-9 in brain tissue lysate with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "MMP-9" }, { "field": "sample_type", "op": "=", "value": "brain tissue lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000373": { "query": "Find immunoblotting experiments detecting CTSB in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "CTSB" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000921": { "query": "Find immunoblotting experiments detecting ADORA2A in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ADORA2A" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_001650": { "query": "Find immunoblotting experiments detecting NCOA4 in total protein extract with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "NCOA4" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_002008": { "query": "Find immunoblotting experiments detecting IL-1β in protein extracts with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_002303": { "query": "Find immunoblotting experiments detecting ZEB1 in cell lysate with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "ZEB1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002775": { "query": "Find immunoblotting experiments detecting c-Myc in total protein extract with 50 μg loaded.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "c-Myc" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_000281": { "query": "Find immunoblotting experiments detecting TERT with membranes blocked using 5% BSA before antibody incubation.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "TERT" } ], "explore_field": null }, "immunotechnology_R2_pos_000849": { "query": "Find immunoblotting experiments detecting LC3 in cell lysates prepared with SDS lysis buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "SDS lysis buffer" }, { "field": "protein_targets.target", "op": "=", "value": "LC3" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000464": { "query": "Find immunoblotting experiments detecting URAT1 in total protein extracts prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "URAT1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_000531": { "query": "Find immunoblotting experiments detecting PERK with membranes blocked in 5% non-fat milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "non-fat milk" }, { "field": "protein_targets.target", "op": "=", "value": "PERK" } ], "explore_field": null }, "immunotechnology_R2_pos_001048": { "query": "Find immunoblotting experiments detecting AKT in cell lysates prepared with SDS lysis buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "SDS lysis buffer" }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000824": { "query": "Find immunoblotting experiments detecting PUMA in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PUMA" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002186": { "query": "Find immunoblotting experiments detecting MBP in tissue lysates with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "MBP" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_001651": { "query": "Find immunoblotting experiments detecting CD63 in total protein extract with 20 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "CD63" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_001613": { "query": "Find immunoblotting experiments detecting MMP-13 in total protein extract with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "protein_targets.target", "op": "=", "value": "MMP-13" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R2_pos_001206": { "query": "Find immunoblotting experiments detecting p38 in cell lysates prepared with Laemmli buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "Laemmli buffer" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000583": { "query": "Find immunoblotting experiments detecting SNAI2 in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "SNAI2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_000221": { "query": "Find immunoblotting experiments detecting YBX1 with membranes blocked in 5% nonfat dry milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "YBX1" } ], "explore_field": null }, "immunotechnology_R1_pos_000984": { "query": "Find immunoblotting experiments detecting MFF with membranes blocked in 5% BSA before antibody incubation.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "MFF" } ], "explore_field": null }, "immunotechnology_R3_pos_001855": { "query": "Find immunoblotting experiments detecting BECN1 in tissue lysates with 20 μg loaded per lane and skim milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "BECN1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000705": { "query": "Find immunoblotting experiments detecting Hexokinase 2 in total protein extract using 5% nonfat dry milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "Hexokinase 2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_002765": { "query": "Find immunoblotting experiments detecting ERK1/2 in cell lysate samples, with 12 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "12 μg", "parsed": { "kind": "point", "number": 12.0, "unit": "ug", "raw": "12 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1/2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002910": { "query": "Find immunoblotting experiments detecting VCAM1 in cell lysate with beta-actin as the normalization control and 10-20 μg loading amount.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 10.0, "upper": 20.0, "unit": "μg" }, "surface": "10-20 μg" }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "VCAM1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003228": { "query": "Find immunoblotting experiments detecting JNK in cell lysate with 50 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "JNK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003185": { "query": "Find immunoblotting experiments detecting GSDMD in total protein extracts with beta-actin as the normalization control and a loading amount of 30-50 μg/lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg/lane" }, "surface": "30-50 μg/lane" }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "GSDMD" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_002194": { "query": "Find immunoblotting experiments detecting ATG7 in cell lysate with 40-60 μg loaded and BSA used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 60.0, "unit": "μg" }, "surface": "40-60 μg" }, { "field": "protein_targets.target", "op": "=", "value": "ATG7" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_001613": { "query": "Find immunoblotting experiments detecting AKT in cell lysate with 50 μg/lane loaded and non-fat milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat milk" }, { "field": "loading_amount", "op": "=", "value": "50 μg/lane", "parsed": { "kind": "point", "number": 50.0, "unit": "ug/lane", "raw": "50 μg/lane" } }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002711": { "query": "Find immunoblotting experiments detecting CDK4 in cell lysate with 20 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "CDK4" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_000097": { "query": "Find immunoblotting experiments detecting c-Jun in cell lysates prepared with RIPA buffer and loaded at 20 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "c-Jun" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002287": { "query": "Find immunoblotting experiments detecting caspase-3 in tissue homogenates, with 50-100 μg loaded and nonfat dry milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 100.0, "unit": "μg" }, "surface": "50-100 μg" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-3" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": null }, "immunotechnology_R3_pos_001911": { "query": "Find immunoblotting experiments detecting RELA in cell lysate samples with 30 μg loaded and BSA used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "RELA" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000508": { "query": "Find immunoblotting experiments detecting Ki-67 in cell lysate with 5% skim milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "Ki-67" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000012": { "query": "Find immunoblotting experiments detecting E-cadherin in total protein lysate with membranes blocked in 5% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "E-cadherin" }, { "field": "sample_type", "op": "=", "value": "total protein lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000979": { "query": "Find immunoblotting experiments detecting CD206 in cell lysate using 5% skim milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "CD206" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_001220": { "query": "Find immunoblotting experiments detecting caspase-1 in total protein extract using 5% nonfat milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "Nonfat milk" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_003377": { "query": "Find immunoblotting experiments detecting AKT in cell lysates with 10 μg loaded per lane and beta-actin as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_000155": { "query": "Find immunoblotting experiments detecting PCNA in tissue lysates prepared with RIPA buffer and loaded at 20 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PCNA" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000437": { "query": "Find immunoblotting experiments detecting GSK3B in tissue lysate using 5% nonfat dry milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "GSK3B" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000781": { "query": "Find immunoblotting experiments detecting GLI1 in cell lysate using 5% non-fat dry milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "non-fat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "GLI1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002337": { "query": "Find immunoblotting experiments detecting VIM in total protein extract with 30 μg loaded and beta-actin as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "VIM" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_002082": { "query": "Find immunoblotting experiments detecting PCNA in total protein extract with 25-30 μg loading and skim milk blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "between", "range": { "lower": 25.0, "upper": 30.0, "unit": "μg" }, "surface": "25-30 μg" }, { "field": "protein_targets.target", "op": "=", "value": "PCNA" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_001771": { "query": "Find immunoblotting experiments detecting BAX in tissue lysate samples, with 30 μg loaded per lane and nonfat dry milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "BAX" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000142": { "query": "Find immunoblotting experiments detecting IκBα in tissue homogenates with membranes blocked in 5% BSA.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "IκBα" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": null }, "immunotechnology_R3_pos_002370": { "query": "Find immunoblotting experiments detecting CDK6 in cell lysate with 30 μg loaded per lane and beta-actin as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "CDK6" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002077": { "query": "Find immunoblotting experiments detecting p65 in total protein extract with 30 μg loading and BSA blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "p65" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_002786": { "query": "Find immunoblotting experiments detecting PARP1 in cell lysate samples with 25 μg loaded and beta-actin used as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "PARP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000609": { "query": "Find immunoblotting experiments detecting NLRP3 in tissue samples using 5% BSA as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "tissue" } ], "explore_field": null }, "immunotechnology_R3_pos_001746": { "query": "Find immunoblotting experiments detecting JNK in cell lysate with 30 μg loaded and milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "milk" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "JNK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003519": { "query": "Find immunoblotting experiments detecting IGF1R in tissue lysates with 40 μg loaded and beta-actin used as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "IGF1R" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000027": { "query": "Find immunoblotting experiments detecting SP1 in cell lysate using 5% skim milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "SP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003055": { "query": "Find immunoblotting experiments detecting KEAP1 in cell lysate with GAPDH as the normalization control and 20-30 μg loading amount.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 30.0, "unit": "μg" }, "surface": "20-30 μg" }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "KEAP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_000244": { "query": "Find immunoblotting experiments detecting CLDN1 in total protein extract prepared with RIPA buffer and loaded at 25 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "CLDN1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI5_pos_000204": { "query": "What blocking solution is used for Western blot detection of MYOG in RIPA-prepared cell lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "MYOG" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000827": { "query": "What normalization control is used for detecting BECN1 in 15 μg of RIPA-buffer cell lysate?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "15 μg", "parsed": { "kind": "point", "number": 15.0, "unit": "ug", "raw": "15 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "BECN1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000399": { "query": "What blocking solution is used for Western blot detection of p65 in RIPA-prepared cell lysates loaded at 25 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "p65" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000558": { "query": "What blocking solution is used for Western blot detection of FPN1 in RIPA-prepared cell lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "FPN1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000065": { "query": "What blocking solution is used for Western blot detection of FTH1 in total protein extract prepared with RIPA buffer and loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "FTH1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000771": { "query": "What normalization control is used for Western blot detection of PI3K p85 in RIPA-prepared tissue lysates loaded at 15-30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 15.0, "upper": 30.0, "unit": "μg" }, "surface": "15-30 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PI3K p85" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000076": { "query": "What blocking solution is used for Western blot detection of NFKB1 in RIPA buffer-prepared cell lysates loaded at 30-40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 40.0, "unit": "μg" }, "surface": "30-40 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "NFKB1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000162": { "query": "What blocking solution is used for Western blot detection of COX-2 in total protein extract prepared with RIPA buffer and loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "COX-2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000122": { "query": "What blocking solution is used for Western blot detection of SNAI1 in RIPA buffer-prepared tissue lysates loaded at 30-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "SNAI1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000152": { "query": "What blocking solution is used for Western blot detection of NF-κB in RIPA buffer-prepared tissue lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "NF-κB" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000919": { "query": "What normalization control is used for Western blot detection of COX-2 in RIPA buffer-prepared cell lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "COX-2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000986": { "query": "What normalization control is used for Western blot detection of CDH2 in RIPA buffer-prepared cell lysates loaded at 25 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg/lane", "parsed": { "kind": "point", "number": 25.0, "unit": "ug/lane", "raw": "25 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "CDH2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000579": { "query": "What blocking solution is used for Western blot detection of caspase-1 in RIPA buffer-prepared tissue lysates loaded at 40-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 50.0, "unit": "μg" }, "surface": "40-50 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000567": { "query": "What blocking solution is used for Western blot detection of Iba1 in RIPA-prepared cell lysates loaded at 5-30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 5.0, "upper": 30.0, "unit": "μg" }, "surface": "5-30 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "Iba1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000798": { "query": "What normalization control is used for Western blot detection of SMAD3 in RIPA buffer-prepared cell lysates loaded at 20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "SMAD3" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000778": { "query": "What normalization control is used for Western blot detection of caspase-3 in RIPA-buffer cytoplasmic extracts loaded at 25-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 25.0, "upper": 50.0, "unit": "μg" }, "surface": "25-50 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-3" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000631": { "query": "What normalization control is used for ERK Western blotting in RIPA-prepared cell lysate loaded at 20 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ERK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000836": { "query": "What normalization control is used for Western blot detection of caspase-1 in RIPA buffer-prepared tissue homogenates loaded at 40-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 50.0, "unit": "μg" }, "surface": "40-50 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-1" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000437": { "query": "What blocking solution is used for Western blot detection of GSDMD in RIPA buffer-prepared tissue lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "GSDMD" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000921": { "query": "What normalization control is used for ERK Western blotting in RIPA-prepared cell lysates loaded at 30 μg per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ERK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000271": { "query": "What blocking solution is used for Western blot detection of ZO-1 in RIPA buffer-prepared tissue lysates with 40-80 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 80.0, "unit": "μg" }, "surface": "40-80 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ZO-1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000429": { "query": "What blocking solution is used for Western blot detection of GSDMD in RIPA-prepared cell lysates loaded at 40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "GSDMD" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI_NEG_neg_006856": { "query": "What normalization control other than PCNA is used for Western blot detection of SLC7A11 in tissue lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "PCNA" }, { "field": "protein_targets.target", "op": "=", "value": "SLC7A11" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_006650": { "query": "What normalization control other than lamin B1 is used for Western blot detection of PARP in cell lysate with 30 μg loaded per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "PARP" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_012534": { "query": "What normalization control other than ERK is used for Western blot detection of MCL1 in cell lysate with a loading amount of 40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "!=", "value": "ERK" }, { "field": "protein_targets.target", "op": "=", "value": "MCL1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014805": { "query": "What normalization control other than lamin B1 is used for Western blot detection of caspase-4 in cell lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-4" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_016093": { "query": "What normalization control other than VDAC is used for Western blot detection of SLC7A11 in tissue lysate with 30-50 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "normalization_control", "op": "!=", "value": "VDAC" }, { "field": "protein_targets.target", "op": "=", "value": "SLC7A11" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_007260": { "query": "What normalization control other than beta-actin is used for Western blot detection of MMP-13 in cell lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "MMP-13" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_007135": { "query": "What normalization control other than gamma-tubulin is used for Western blot detection of NRF2 in cytoplasmic extract with 50-100 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 100.0, "unit": "μg" }, "surface": "50-100 μg" }, { "field": "normalization_control", "op": "!=", "value": "gamma-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "NRF2" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_011959": { "query": "What normalization control other than beta-actin is used for Western blot detection of WNT3A in cell lysate with 30 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "WNT3A" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI3_pos_000312": { "query": "What normalization control is used for Western blot detection of LC3 in cell lysate loaded at 12-15 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 12.0, "upper": 15.0, "unit": "μg" }, "surface": "12-15 μg" }, { "field": "protein_targets.target", "op": "=", "value": "LC3" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_005004": { "query": "What normalization control other than lamin B1 is used for Western blot detection of IκBα in tissue lysate with 20 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "IκBα" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_018203": { "query": "What normalization control other than actin is used for Western blot detection of p62 in whole cell lysate with 15-100 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 15.0, "upper": 100.0, "unit": "μg" }, "surface": "15-100 μg" }, { "field": "normalization_control", "op": "!=", "value": "actin" }, { "field": "protein_targets.target", "op": "=", "value": "p62" }, { "field": "sample_type", "op": "=", "value": "whole cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_006550": { "query": "What normalization control other than H3 is used for Western blot detection of C/EBPβ in cell lysate with 20 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "C/EBPβ" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014071": { "query": "What normalization control other than H3 is used for Western blot detection of PI3K in cell lysate loaded at 20 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "PI3K" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_009575": { "query": "What normalization control other than beta-tubulin is used for Western blot detection of CDKN1A in cell lysate loaded at 30 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg/lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "beta-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "CDKN1A" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_012519": { "query": "What normalization control other than tubulin is used for Western blot detection of MCL1 in cell lysate with 40 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "!=", "value": "tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "MCL1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_009334": { "query": "What normalization control other than ERK is used for Western blot detection of p38 in total protein extract loaded at 30 μg per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "ERK" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_007121": { "query": "What normalization control other than GAPDH is used for Western blot detection of NRF2 in cytoplasmic extract with 50-100 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 100.0, "unit": "μg" }, "surface": "50-100 μg" }, { "field": "normalization_control", "op": "!=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "NRF2" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_017516": { "query": "What normalization control other than lamin B is used for Western blot detection of LC3B in tissue lysate with 50 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B" }, { "field": "protein_targets.target", "op": "=", "value": "LC3B" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_009602": { "query": "What normalization control other than lamin A/C is used for Western blot detection of AKT in tissue lysate loaded at 20 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "lamin A/C" }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014421": { "query": "What normalization control other than GAPDH is used for Western blot detection of GPX4 in total protein extract with 20-30 μg loading amount?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 30.0, "unit": "μg" }, "surface": "20-30 μg" }, { "field": "normalization_control", "op": "!=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "GPX4" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_002491": { "query": "What normalization control other than beta-tubulin is used for Western blot detection of IL-1β in total protein extract loaded at 25 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_007166": { "query": "What normalization control other than lamin B is used for Western blot detection of HO-1 in tissue lysate loaded at 20-40 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 40.0, "unit": "μg/lane" }, "surface": "20-40 μg/lane" }, { "field": "normalization_control", "op": "!=", "value": "lamin B" }, { "field": "protein_targets.target", "op": "=", "value": "HO-1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI1_pos_000581": { "query": "What blocking solution is used for Western blot detection of DMT1 in cell lysate?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "DMT1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI1_pos_000746": { "query": "What blocking solution is used for Western blot detection of CCR7 in tissue lysate?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "CCR7" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI1_pos_000674": { "query": "What blocking solution is used for Western blot detection of POU5F1 in tissue lysate?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "POU5F1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI_NEG_neg_004941": { "query": "What normalization control other than actin is used for Western blot detection of PARP in cell lysate with 40 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "!=", "value": "actin" }, { "field": "protein_targets.target", "op": "=", "value": "PARP" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_003904": { "query": "What normalization control other than TBP is used for Western blot detection of p38 in total protein extract loaded at 30 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg/lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "TBP" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_006649": { "query": "What normalization control other than H3 is used for Western blot detection of PARP in cell lysate loaded at 30 μg per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "PARP" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_003433": { "query": "What normalization control other than beta-actin is used for Western blot detection of BCL2 in cell lysate with 10 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "BCL2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_017435": { "query": "What normalization control other than beta-tubulin is used for Western blot detection of RELA in cytoplasmic extract loaded at 50-100 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 100.0, "unit": "μg" }, "surface": "50-100 μg" }, { "field": "normalization_control", "op": "!=", "value": "beta-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "RELA" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014379": { "query": "What normalization control other than gamma-tubulin is used for Western blot detection of CD9 in cell lysate loaded at 10 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "!=", "value": "gamma-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "CD9" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_009946": { "query": "What normalization control other than VDAC is used for Western blot detection of ZO-1 in cell lysate loaded at 20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "VDAC" }, { "field": "protein_targets.target", "op": "=", "value": "ZO-1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_011597": { "query": "What normalization control other than COX4 is used for Western blot detection of BAX in total protein lysate loaded at 30-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "normalization_control", "op": "!=", "value": "COX4" }, { "field": "protein_targets.target", "op": "=", "value": "BAX" }, { "field": "sample_type", "op": "=", "value": "total protein lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_008723": { "query": "What normalization control other than alpha-tubulin is used for Western blot detection of caspase-9 in tissue lysate loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "alpha-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-9" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_003909": { "query": "What normalization control other than ERK is used for Western blot detection of p38 in total protein extract loaded at 30 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg/lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "ERK" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_007323": { "query": "What normalization control other than H3 is used for Western blot detection of p70S6K in total protein extract loaded at 40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "p70S6K" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014504": { "query": "What normalization control other than beta-tubulin is used for Western blot detection of GRP78 in cell lysate with a loading amount of 40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "GRP78" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000883": { "query": "What normalization control is used for Western blot detection of β-catenin in RIPA buffer-prepared cell lysates loaded at 25 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg/lane", "parsed": { "kind": "point", "number": 25.0, "unit": "ug/lane", "raw": "25 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "β-catenin" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_003898": { "query": "What normalization control other than lamin B is used for Western blot detection of p38 in total protein extract loaded at 30 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg/lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_015144": { "query": "What normalization control other than PCNA is used for Western blot detection of E-cadherin with 25 μg of total protein extract loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "normalization_control", "op": "!=", "value": "PCNA" }, { "field": "protein_targets.target", "op": "=", "value": "E-cadherin" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_010048": { "query": "What normalization control other than GAPDH is used for Western blot detection of RELA in nuclear extract loaded at 60 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "60 μg", "parsed": { "kind": "point", "number": 60.0, "unit": "ug", "raw": "60 μg" } }, { "field": "normalization_control", "op": "!=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "RELA" }, { "field": "sample_type", "op": "=", "value": "nuclear extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI3_pos_001466": { "query": "What blocking solution is used for Western blot detection of NF-κB in tissue homogenate samples loaded at 20-40 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 40.0, "unit": "μg" }, "surface": "20-40 μg" }, { "field": "protein_targets.target", "op": "=", "value": "NF-κB" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI5_pos_000499": { "query": "What blocking solution is used for Western blot detection of BAX in RIPA buffer-prepared cell lysates loaded at 50 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg/lane", "parsed": { "kind": "point", "number": 50.0, "unit": "ug/lane", "raw": "50 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "BAX" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI_NEG_neg_010119": { "query": "What normalization control other than PCNA is used for Western blot detection of NRF2 in tissue lysate with 30 μg loaded per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "PCNA" }, { "field": "protein_targets.target", "op": "=", "value": "NRF2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EC5_pos_000028": { "query": "What gel percentage is used for Western blot detection of PCNA in RIPA-prepared cell lysates loaded at 30 μg per lane?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PCNA" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000223": { "query": "What gel percentage is used to detect Collagen III in RIPA buffer-prepared tissue lysates loaded at 30 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "Collagen III" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000289": { "query": "What gel percentage is used to detect LC3B in RIPA-prepared cell lysates loaded at 25 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "LC3B" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000126": { "query": "What gel percentage is used for Western blot detection of ERK1/2 in RIPA-prepared cell lysate loaded at 50 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1/2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000049": { "query": "What gel percentage is used to detect iNOS in total protein extract prepared with RIPA buffer when loading 30 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "iNOS" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000025": { "query": "What gel percentage is used to detect E-cadherin in total protein extract prepared with RIPA buffer when loading 30 μg per lane?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "E-cadherin" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000218": { "query": "What gel percentage is used to detect HO-1 in RIPA-prepared tissue lysates loaded at 20-40 μg/lane?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 40.0, "unit": "μg/lane" }, "surface": "20-40 μg/lane" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "HO-1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000104": { "query": "What gel percentage is used for Western blot detection of PKA in 30 μg RIPA-buffer tissue lysate samples?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PKA" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000314": { "query": "What gel percentage is used to detect β-catenin in RIPA-prepared cell lysate with 20 μg/lane loading?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "β-catenin" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000093": { "query": "What gel percentage is used to detect BCL2 in tissue lysates prepared with RIPA buffer when loading 30 μg/lane?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg/lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "BCL2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000264": { "query": "What gel percentage is used to detect IL-1β in 40 μg of total protein extract prepared with RIPA buffer?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000039": { "query": "What gel percentage is used to detect MMP-13 in total protein extract prepared with RIPA buffer when loading 30 μg per lane?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "MMP-13" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC5_pos_000370": { "query": "What gel percentage is used to detect HO-1 in RIPA-prepared tissue lysates with 30 μg loaded?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "HO-1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC3_pos_001066": { "query": "What gel percentage is used to detect IL-1β in tissue lysate with a loading amount of 40 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC4_pos_000496": { "query": "What loading amount is used for Western blot detection of TH in RIPA buffer-prepared cell lysates?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC4", "constraint_count": 4, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "TH" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "loading_amount" }, "immunotechnology_EC3_pos_000705": { "query": "What gel percentage is used to detect IL-6 with 5% non-fat dry milk as the blocking solution?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "non-fat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "IL-6" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC3_pos_000816": { "query": "What gel percentage is used to detect VIM in cell lysate when loading 10 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "VIM" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC4_pos_000486": { "query": "What loading amount is used for Western blot detection of AR in RIPA buffer-prepared cell lysates?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC4", "constraint_count": 4, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "AR" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "loading_amount" }, "immunotechnology_EC3_pos_001168": { "query": "What gel percentage is used to detect p62 in total protein extract when loading 25 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "p62" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC4_pos_000831": { "query": "What gel percentage is used to detect ERK1 in total protein extract prepared with RIPA buffer?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC4", "constraint_count": 4, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC3_pos_001000": { "query": "What gel percentage is used to detect SNAI1 in cell lysate with a loading amount of 20 μg?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "SNAI1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC4_pos_000915": { "query": "What gel percentage is used to detect PSD-95 in brain tissue lysate prepared with RIPA buffer?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC4", "constraint_count": 4, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PSD-95" }, { "field": "sample_type", "op": "=", "value": "brain tissue lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC1_pos_001239": { "query": "What gel percentage is used to detect SOX9 in a Western blot blocked with skim milk?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "SOX9" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC1_pos_000891": { "query": "What loading amount is used for Western blot detection of β-catenin in whole-cell lysate?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "β-catenin" }, { "field": "sample_type", "op": "=", "value": "whole-cell lysate" } ], "explore_field": "loading_amount" }, "immunotechnology_EC1_pos_002431": { "query": "What gel percentage is used to detect XBP1 in total protein extract?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "XBP1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC1_pos_000691": { "query": "What loading amount is used for Western blot detection of BECN1 in tissue protein extract?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "BECN1" }, { "field": "sample_type", "op": "=", "value": "tissue protein extract" } ], "explore_field": "loading_amount" }, "immunotechnology_EC1_pos_001526": { "query": "What gel percentage is used for Western blot detection of DVL2 when BSA is used as the blocking solution?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "DVL2" } ], "explore_field": "gel_percentage" }, "immunotechnology_EC1_pos_002127": { "query": "What gel percentage is used to detect TRX1 in cell lysate?", "domain": "immunotechnology", "type": "condition_exploration", "type_id": "EC1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "TRX1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "gel_percentage" }, "immunotechnology_EM5_pos_000091": { "query": "What lysis buffer is used for Western blot detection of TGFB1 in cell lysate when membranes are blocked with 5% nonfat dry milk?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "TGFB1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM5_pos_000010": { "query": "What lysis buffer is used for Western blot detection of RIPK1 in cell lysate when membranes are blocked with 5% skim milk?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "RIPK1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM5_pos_000118": { "query": "What lysis buffer is used to prepare cell lysates loaded at 30 μg per lane for Western blot detection of GPX4 with skim milk blocking?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "protein_targets.target", "op": "=", "value": "GPX4" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM5_pos_000191": { "query": "What lysis buffer is used to prepare total protein extract for Western blot detection of ERK1/2 with 50 μg/lane loading and GAPDH normalization?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg/lane", "parsed": { "kind": "point", "number": 50.0, "unit": "ug/lane", "raw": "50 μg/lane" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1/2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM1_pos_000182": { "query": "What lysis buffer is used to prepare cell lysates for detecting APJ by Western blot?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "APJ" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM1_pos_000231": { "query": "What lysis buffer is used to prepare cell lysates for detecting Hexokinase 1?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "Hexokinase 1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM1_pos_000187": { "query": "What lysis buffer is used to prepare cytoplasmic fractions for Western blot detection of p38?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM1", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic fraction" } ], "explore_field": "lysis_buffer" }, "immunotechnology_EM4_pos_000277": { "query": "What lysis buffer is used to detect VP1 in cell lysates normalized to beta-actin?", "domain": "immunotechnology", "type": "method_exploration", "type_id": "EM4", "constraint_count": 4, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "VP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "lysis_buffer" }, "immunotechnology_R3_pos_001978": { "query": "Find immunoblotting experiments detecting BAX in cell lysates with 40 μg/lane loaded and non-fat milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat milk" }, { "field": "loading_amount", "op": "=", "value": "40 μg/lane", "parsed": { "kind": "point", "number": 40.0, "unit": "ug/lane", "raw": "40 μg/lane" } }, { "field": "protein_targets.target", "op": "=", "value": "BAX" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000952": { "query": "Find immunoblotting experiments detecting NLRP3 in cytoplasmic extracts using 5% skim milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": null }, "immunotechnology_R1_pos_001726": { "query": "Find immunoblotting experiments detecting ATG5 in cell lysate with 35 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "35 μg", "parsed": { "kind": "point", "number": 35.0, "unit": "ug", "raw": "35 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "ATG5" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_000023": { "query": "Find immunoblotting experiments detecting BECN1 in spinal cord tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "BECN1" }, { "field": "sample_type", "op": "=", "value": "spinal cord tissue lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_000799": { "query": "Find immunoblotting experiments detecting WNT3A in cell lysates prepared with RIPA buffer and loaded at 20 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "WNT3A" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_001037": { "query": "Find immunoblotting experiments detecting JNK in cell lysates prepared with RIPA buffer and loaded at 20 μg/lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "JNK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_000314": { "query": "Find immunoblotting experiments detecting p62 in brain tissue lysates using 5% skim milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "p62" }, { "field": "sample_type", "op": "=", "value": "brain tissue lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_005016": { "query": "What normalization control other than ERK is used for Western blot detection of IκBα in tissue lysate loaded at 20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "ERK" }, { "field": "protein_targets.target", "op": "=", "value": "IκBα" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014087": { "query": "What normalization control other than actin is used for ERK Western blotting from cell lysate loaded at 20 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg/lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "actin" }, { "field": "protein_targets.target", "op": "=", "value": "ERK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_003438": { "query": "Find immunoblotting experiments detecting caspase-9 in cell lysate samples with 10 μg loaded and GAPDH used as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-9" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_017813": { "query": "What normalization control other than H3 is used for Western blot detection of CDH2 in cell lysate loaded at 25 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg/lane", "parsed": { "kind": "point", "number": 25.0, "unit": "ug/lane", "raw": "25 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "CDH2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_006628": { "query": "What normalization control other than H3 is used for Western blot detection of NOS3 in tissue lysate with 20 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "H3" }, { "field": "protein_targets.target", "op": "=", "value": "NOS3" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_003464": { "query": "Find immunoblotting experiments detecting caspase-9 in tissue lysates, with 10-20 μg loaded and beta-actin used as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 10.0, "upper": 20.0, "unit": "μg" }, "surface": "10-20 μg" }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-9" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_009935": { "query": "What normalization control other than tubulin is used for Western blot detection of ZO-1 in cell lysate loaded at 20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "ZO-1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R1_pos_002441": { "query": "Find immunoblotting experiments detecting NLRP3 in tissue homogenates with 40-50 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 50.0, "unit": "μg" }, "surface": "40-50 μg" }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_018357": { "query": "What normalization control other than PCNA is used for Western blot detection of p62 in whole cell lysate with 20 μg loading amount?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "PCNA" }, { "field": "protein_targets.target", "op": "=", "value": "p62" }, { "field": "sample_type", "op": "=", "value": "whole cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001875": { "query": "Find immunoblotting experiments detecting MMP-2 in total protein extract with 20 μg loaded and skim milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "MMP-2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_014378": { "query": "What normalization control other than PCNA is used for Western blot detection of CD9 in cell lysate loaded at 10 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "!=", "value": "PCNA" }, { "field": "protein_targets.target", "op": "=", "value": "CD9" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R1_pos_000354": { "query": "Find immunoblotting experiments detecting caspase-3 with membranes blocked in 4% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "4%", "parsed": { "kind": "point", "number": 4.0, "unit": "%", "raw": "4%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-3" } ], "explore_field": null }, "immunotechnology_R3_pos_001860": { "query": "Find immunoblotting experiments detecting IL-1β in cell lysates with 15 μg loaded per lane and BSA used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "15 μg", "parsed": { "kind": "point", "number": 15.0, "unit": "ug", "raw": "15 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_013238": { "query": "What normalization control other than actin is used for Western blot detection of VCAM1 in cell lysate when loading 10-20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 10.0, "upper": 20.0, "unit": "μg" }, "surface": "10-20 μg" }, { "field": "normalization_control", "op": "!=", "value": "actin" }, { "field": "protein_targets.target", "op": "=", "value": "VCAM1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_003290": { "query": "Find immunoblotting experiments detecting ERK in cell lysates with 30 μg loaded per lane and alpha-tubulin as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "alpha-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "ERK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_001788": { "query": "Find immunoblotting experiments detecting OCLN in total protein extract with 40 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "OCLN" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_014409": { "query": "What normalization control other than lamin B is used for Western blot detection of VE-cadherin in cell lysate with 20 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B" }, { "field": "protein_targets.target", "op": "=", "value": "VE-cadherin" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_002229": { "query": "Find immunoblotting experiments detecting CD63 in cell lysate samples, with 10-20 μg loaded and milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "milk" }, { "field": "loading_amount", "op": "between", "range": { "lower": 10.0, "upper": 20.0, "unit": "μg" }, "surface": "10-20 μg" }, { "field": "protein_targets.target", "op": "=", "value": "CD63" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_007129": { "query": "What normalization control other than calnexin is used for Western blot detection of NRF2 in cytoplasmic extract loaded at 50-100 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 100.0, "unit": "μg" }, "surface": "50-100 μg" }, { "field": "normalization_control", "op": "!=", "value": "calnexin" }, { "field": "protein_targets.target", "op": "=", "value": "NRF2" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001219": { "query": "Find immunoblotting experiments detecting NLRP3 in total protein extract with membranes blocked in 5% nonfat milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "Nonfat milk" }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_003069": { "query": "Find immunoblotting experiments detecting RUNX2 in cell lysate with 30 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "RUNX2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002113": { "query": "Find immunoblotting experiments detecting PD-L1 in cell lysate samples, with 20-100 μg loaded per lane and BSA used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 100.0, "unit": "μg" }, "surface": "20-100 μg" }, { "field": "protein_targets.target", "op": "=", "value": "PD-L1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_004186": { "query": "What normalization control other than alpha-tubulin is used for Western blot detection of NLRP3 in total protein extract loaded at 30-50 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg/lane" }, "surface": "30-50 μg/lane" }, { "field": "normalization_control", "op": "!=", "value": "alpha-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000219": { "query": "What blocking solution is used for Western blot detection of fibronectin in RIPA-prepared cell lysates loaded at 50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "Fibronectin" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_R1_pos_002568": { "query": "Find immunoblotting experiments detecting STAT3 in tissue lysates with 50-60 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 50.0, "upper": 60.0, "unit": "μg" }, "surface": "50-60 μg" }, { "field": "protein_targets.target", "op": "=", "value": "STAT3" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_001381": { "query": "Find immunoblotting experiments detecting SRC in cell lysate using 5% skim milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "SRC" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_011683": { "query": "What normalization control other than beta-actin is used for Western blot detection of IL-6 in tissue lysate with 30–60 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 60.0, "unit": "μg" }, "surface": "30-60 μg" }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "IL-6" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R1_pos_002753": { "query": "Find immunoblotting experiments detecting CREB1 in tissue lysates with 25 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "CREB1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_011942": { "query": "What normalization control other than actin is used for Western blot detection of CLDN5 in cell lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "actin" }, { "field": "protein_targets.target", "op": "=", "value": "CLDN5" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001852": { "query": "Find immunoblotting experiments detecting AKT in tissue homogenate samples with 30 μg loaded and non-fat dry milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat dry milk" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "tissue homogenate" } ], "explore_field": null }, "immunotechnology_R1_pos_002986": { "query": "Find immunoblotting experiments detecting PKA in tissue lysates with 20-30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 30.0, "unit": "μg" }, "surface": "20-30 μg" }, { "field": "protein_targets.target", "op": "=", "value": "PKA" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_001797": { "query": "Find immunoblotting experiments detecting SLC7A11 in tissue lysates with 30-50 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "protein_targets.target", "op": "=", "value": "SLC7A11" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_011972": { "query": "What normalization control other than TBP is used for Western blot detection of WNT3A in cell lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "TBP" }, { "field": "protein_targets.target", "op": "=", "value": "WNT3A" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001247": { "query": "Find immunoblotting experiments detecting Iba1 in total protein extracts using 5% BSA as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "Iba1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_001910": { "query": "What normalization control other than alpha-tubulin is used for Western blot detection of KEAP1 in cell lysate with 30 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "alpha-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "KEAP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_000814": { "query": "Find immunoblotting experiments detecting Notch1 in cell lysates with membranes blocked in 5% nonfat dry milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "Notch1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI3_pos_001002": { "query": "What blocking solution is used for Western blot detection of RELA with 20 μg protein extract loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "RELA" }, { "field": "sample_type", "op": "=", "value": "protein extract" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_R3_pos_002167": { "query": "Find immunoblotting experiments detecting ATG5 in total protein extract with 20-60 μg loaded and non-fat milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat milk" }, { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 60.0, "unit": "μg" }, "surface": "20-60 μg" }, { "field": "protein_targets.target", "op": "=", "value": "ATG5" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R4_pos_000550": { "query": "Find immunoblotting experiments detecting PARP in cell lysates prepared with RIPA buffer and loaded at 100 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "100 μg", "parsed": { "kind": "point", "number": 100.0, "unit": "ug", "raw": "100 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "PARP" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003182": { "query": "Find immunoblotting experiments detecting SMAD3 in total protein extract, with 30 μg loaded and beta-actin used as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "SMAD3" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_018362": { "query": "What normalization control other than beta-actin is used for Western blot detection of ERK1/2 in cell lysate with 40 μg/lane loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg/lane", "parsed": { "kind": "point", "number": 40.0, "unit": "ug/lane", "raw": "40 μg/lane" } }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "ERK1/2" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI5_pos_000144": { "query": "What blocking solution is used for Western blot detection of 4E-BP1 in RIPA-prepared cell lysates loaded at 20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "4E-BP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI_NEG_neg_002678": { "query": "What normalization control other than Na+/K+-ATPase is used for Western blot detection of NFKB1 in cell lysate with 30-40 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 40.0, "unit": "μg" }, "surface": "30-40 μg" }, { "field": "normalization_control", "op": "!=", "value": "Na+/K+-ATPase" }, { "field": "protein_targets.target", "op": "=", "value": "NFKB1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_004229": { "query": "What normalization control other than lamin B1 is used when probing GSDMD in total protein extract loaded at 30-50 μg/lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg/lane" }, "surface": "30-50 μg/lane" }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "GSDMD" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_002498": { "query": "What normalization control other than lamin A/C is used for Western blot detection of IL-1β in total protein extract loaded at 25 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "25 μg", "parsed": { "kind": "point", "number": 25.0, "unit": "ug", "raw": "25 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin A/C" }, { "field": "protein_targets.target", "op": "=", "value": "IL-1β" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R2_pos_000468": { "query": "Find immunoblotting experiments detecting CYP2E1 in tissue lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "CYP2E1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI5_pos_000670": { "query": "What normalization control is used for Western blot detection of TGFB1 in tissue lysates prepared with RIPA buffer when loading 30-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "TGFB1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001877": { "query": "Find immunoblotting experiments detecting BAX in tissue lysates with 30 μg loading and BSA blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "BAX" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI5_pos_000838": { "query": "What normalization control is used for Western blot detection of TNF in RIPA buffer-prepared tissue lysates loaded at 50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI5", "constraint_count": 5, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "TNF" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R4_pos_000791": { "query": "Find immunoblotting experiments detecting FTH1 in cell lysates prepared with RIPA buffer and loaded at 20 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "FTH1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R2_pos_001138": { "query": "Find immunoblotting experiments detecting MYLK in cell lysates prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "MYLK" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_012380": { "query": "What normalization control other than lamin A/C is used for Western blot detection of ACC in total protein extract with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin A/C" }, { "field": "protein_targets.target", "op": "=", "value": "ACC" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001529": { "query": "Find immunoblotting experiments detecting AKT in cell lysate with 20 μg loaded and non-fat dry milk used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat dry milk" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_013240": { "query": "What normalization control other than tubulin is used for Western blot detection of VCAM1 in cell lysate when loading 10-20 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 10.0, "upper": 20.0, "unit": "μg" }, "surface": "10-20 μg" }, { "field": "normalization_control", "op": "!=", "value": "tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "VCAM1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_000915": { "query": "Find immunoblotting experiments detecting ROCK1 in tissue lysates with membranes blocked in 5% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "ROCK1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_016083": { "query": "What normalization control other than lamin B1 is used for Western blot detection of SLC7A11 in tissue lysate loaded at 30-50 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 30.0, "upper": 50.0, "unit": "μg" }, "surface": "30-50 μg" }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "SLC7A11" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_002019": { "query": "Find immunoblotting experiments detecting NLRP3 in total protein extract with 20 μg loaded and non-fat milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "non-fat milk" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "NLRP3" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_002059": { "query": "Find immunoblotting experiments detecting cytochrome c in cell lysate with 20 μg loading and BSA as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "cytochrome c" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R4_pos_000826": { "query": "Find immunoblotting experiments detecting caspase-9 in cell lysates prepared with RIPA buffer and loaded at 10 μg per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R4", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-9" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_004572": { "query": "What normalization control other than gamma-tubulin is used for Western blot detection of AKT in tissue lysate loaded at 20 μg per lane?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg per lane", "parsed": { "kind": "point", "number": 20.0, "unit": "ug/lane", "raw": "20 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "gamma-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "AKT" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_014630": { "query": "What normalization control other than lamin B is used for Western blot detection of TGFB in cell lysate with 30 μg loaded?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B" }, { "field": "protein_targets.target", "op": "=", "value": "TGFB" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_EI_NEG_neg_009331": { "query": "What normalization control other than gamma-tubulin is used for Western blot detection of p38 with 30 μg per lane of total protein extract?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg per lane", "parsed": { "kind": "point", "number": 30.0, "unit": "ug/lane", "raw": "30 μg per lane" } }, { "field": "normalization_control", "op": "!=", "value": "gamma-tubulin" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_000954": { "query": "Find immunoblotting experiments detecting ASC in cytoplasmic extracts with membranes blocked in 5% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "ASC" }, { "field": "sample_type", "op": "=", "value": "cytoplasmic extract" } ], "explore_field": null }, "immunotechnology_EI3_pos_001285": { "query": "What blocking solution is used for Western blot detection of SMAD5 in cell lysate when loading 40-60 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "loading_amount", "op": "between", "range": { "lower": 40.0, "upper": 60.0, "unit": "μg" }, "surface": "40-60 μg" }, { "field": "protein_targets.target", "op": "=", "value": "SMAD5" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "blocking_solution.identity" }, "immunotechnology_EI_NEG_neg_001922": { "query": "What normalization control other than TBP is used for Western blot detection of KEAP1 in cell lysate with 30 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "TBP" }, { "field": "protein_targets.target", "op": "=", "value": "KEAP1" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001206": { "query": "Find immunoblotting experiments detecting BMP2 in total protein extract using 5% BSA as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "BMP2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_001823": { "query": "Find immunoblotting experiments detecting BCL2 in tissue lysates with 50 μg loading and skim milk blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "=", "value": "50 μg", "parsed": { "kind": "point", "number": 50.0, "unit": "ug", "raw": "50 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "BCL2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002683": { "query": "Find immunoblotting experiments detecting WNT3A in cell lysate with 20 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "WNT3A" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003459": { "query": "Find immunoblotting experiments detecting caspase-9 in total protein extract with 20 μg loading and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "caspase-9" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_000186": { "query": "Find immunoblotting experiments detecting LAMP1 with membranes blocked in 5% BSA.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "LAMP1" } ], "explore_field": null }, "immunotechnology_R1_pos_000895": { "query": "Find immunoblotting experiments detecting SPHK1 with membranes blocked in 5% skim milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "protein_targets.target", "op": "=", "value": "SPHK1" } ], "explore_field": null }, "immunotechnology_R3_pos_003213": { "query": "Find immunoblotting experiments detecting JAK2 in total protein extract with 40 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "40 μg", "parsed": { "kind": "point", "number": 40.0, "unit": "ug", "raw": "40 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "JAK2" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_001488": { "query": "What normalization control other than GAPDH is used for Western blot detection of BECN1 in tissue lysate with 20 μg loading?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "normalization_control", "op": "!=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "BECN1" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_001931": { "query": "Find immunoblotting experiments detecting c-Myc in cell lysates with 20-30 μg/lane loaded and skim milk used for blocking.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": true, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "skim milk" }, { "field": "loading_amount", "op": "between", "range": { "lower": 20.0, "upper": 30.0, "unit": "μg/lane" }, "surface": "20-30 μg/lane" }, { "field": "protein_targets.target", "op": "=", "value": "c-Myc" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_002442": { "query": "Find immunoblotting experiments detecting CD63 in cell lysate with 10 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "CD63" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_001706": { "query": "Find immunoblotting experiments detecting LC3 in cell lysate with 20 μg loaded and BSA used as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "LC3" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_002656": { "query": "Find immunoblotting experiments detecting COL1 in total protein extract with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "COL1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R1_pos_002265": { "query": "Find immunoblotting experiments detecting caspase-3 in cell protein extracts with 30 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "caspase-3" }, { "field": "sample_type", "op": "=", "value": "cell protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_001000": { "query": "Find immunoblotting experiments detecting CPT1 in total protein extract using 5% nonfat dry milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "nonfat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "CPT1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_012352": { "query": "What normalization control other than beta-actin is used for Western blot detection of c-Myc in total protein extract loaded at 30 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "!=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "c-Myc" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R3_pos_000245": { "query": "Find immunoblotting experiments detecting STING in tissue lysates using 5% BSA as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "BSA" }, { "field": "protein_targets.target", "op": "=", "value": "STING" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_003240": { "query": "Find immunoblotting experiments detecting p38 in total protein extracts loaded at 10 μg and normalized to GAPDH.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "10 μg", "parsed": { "kind": "point", "number": 10.0, "unit": "ug", "raw": "10 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "p38" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_EI_NEG_neg_012338": { "query": "What normalization control other than lamin B1 is used for Western blot detection of TNF in total protein extract loaded at 15 μg?", "domain": "immunotechnology", "type": "input_exploration", "type_id": "EI_NEG", "constraint_count": 4, "has_numeric": true, "has_negation": true, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "15 μg", "parsed": { "kind": "point", "number": 15.0, "unit": "ug", "raw": "15 μg" } }, { "field": "normalization_control", "op": "!=", "value": "lamin B1" }, { "field": "protein_targets.target", "op": "=", "value": "TNF" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": "normalization_control" }, "immunotechnology_R1_pos_000705": { "query": "Find immunoblotting experiments detecting VEGFR2 with membranes blocked in 5% nonfat milk.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "Nonfat milk" }, { "field": "protein_targets.target", "op": "=", "value": "VEGFR2" } ], "explore_field": null }, "immunotechnology_R3_pos_002708": { "query": "Find immunoblotting experiments detecting SIRT1 in total protein extract with 30 μg loaded and GAPDH as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "GAPDH" }, { "field": "protein_targets.target", "op": "=", "value": "SIRT1" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R3_pos_003347": { "query": "Find immunoblotting experiments detecting IκBα in cell lysate with 30 μg loaded and beta-actin as the normalization control.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "30 μg", "parsed": { "kind": "point", "number": 30.0, "unit": "ug", "raw": "30 μg" } }, { "field": "normalization_control", "op": "=", "value": "beta-actin" }, { "field": "protein_targets.target", "op": "=", "value": "IκBα" }, { "field": "sample_type", "op": "=", "value": "cell lysate" } ], "explore_field": null }, "immunotechnology_R3_pos_001480": { "query": "Find immunoblotting experiments detecting SOD2 in tissue lysate using 5% non-fat dry milk as the blocking solution.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R3", "constraint_count": 4, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "blocking_solution.concentration", "op": "=", "value": "5%", "parsed": { "kind": "point", "number": 5.0, "unit": "%", "raw": "5%" } }, { "field": "blocking_solution.identity", "op": "=", "value": "non-fat dry milk" }, { "field": "protein_targets.target", "op": "=", "value": "SOD2" }, { "field": "sample_type", "op": "=", "value": "tissue lysate" } ], "explore_field": null }, "immunotechnology_R1_pos_001705": { "query": "Find immunoblotting experiments detecting PGC-1α in total protein extract with 20 μg loaded per lane.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R1", "constraint_count": 3, "has_numeric": true, "has_negation": false, "has_range": false, "constraints": [ { "field": "loading_amount", "op": "=", "value": "20 μg", "parsed": { "kind": "point", "number": 20.0, "unit": "ug", "raw": "20 μg" } }, { "field": "protein_targets.target", "op": "=", "value": "PGC-1α" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null }, "immunotechnology_R2_pos_000430": { "query": "Find immunoblotting experiments detecting RhoA in total protein extracts prepared with RIPA buffer.", "domain": "immunotechnology", "type": "reproduction", "type_id": "R2", "constraint_count": 3, "has_numeric": false, "has_negation": false, "has_range": false, "constraints": [ { "field": "lysis_buffer", "op": "=", "value": "RIPA buffer" }, { "field": "protein_targets.target", "op": "=", "value": "RhoA" }, { "field": "sample_type", "op": "=", "value": "total protein extract" } ], "explore_field": null } }