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README.md
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---
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task_categories:
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- keypoint-detection
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license: cc-by-4.0
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tags:
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- biology
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- pose-estimation
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- multiview
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- fly
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- lightning-pose
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pretty_name: Fly Anipose (Lightning Pose subset)
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size_categories:
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- 1K<n<10K
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---
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# Fly Anipose — Lightning Pose Multiview Dataset
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6-camera pose estimation dataset for *Drosophila* leg keypoints, packaged for use with [Lightning Pose](https://github.com/danbider/lightning-pose).
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## Dataset Description
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Head-fixed flies run on a spherical treadmill while 6 synchronized cameras capture locomotion at **300 Hz**. Each frame is labeled with **30 keypoints** — 5 joint segments (A–E) on each of 6 legs (left legs L1–L3, right legs R1–R3).
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Labels are **filtered Anipose predictions**, not hand-labeled frames. They were constructed by:
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1. Removing instances with mean 3D reprojection error > 10 px
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2. Running k-means on 3D poses and keeping 25 instances per session
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3. Using filtered 2D predictions; setting keypoints with 2D reprojection error > 10 px to NaN
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Source data: Karashchuk et al., *Cell Reports* 2021 — original archive at https://doi.org/10.5061/dryad.nzs7h44s4
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## Data Splits
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| Split | Labeled instances | Sessions |
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|-------|----------------:|--------:|
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| In-distribution (InD) | 377 | 16 |
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| Out-of-distribution (OOD) | 300 | 12 |
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InD and OOD sets contain **different animals/sessions** (no overlap).
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- `CollectedData_Cam-{A-F}.csv` — InD labels; `videos/` — InD videos
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- `CollectedData_Cam-{A-F}_new.csv` — OOD labels; `videos_new/` — OOD videos
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## Keypoints
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30 keypoints total: side (`L`/`R`) + leg number (`1`–`3`) + segment (`A`–`E`, proximal→distal).
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| Left legs | Right legs |
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|-----------|------------|
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| L1A, L1B, L1C, L1D, L1E | R1A, R1B, R1C, R1D, R1E |
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| L2A, L2B, L2C, L2D, L2E | R2A, R2B, R2C, R2D, R2E |
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| L3A, L3B, L3C, L3D, L3E | R3A, R3B, R3C, R3D, R3E |
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## Directory Structure
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```
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fly_anipose_subset/
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├── labeled-data/ # Extracted frames per session×view; includes ±2 context frames
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├── videos/ # Full InD session videos (<SessionKey>_<View>.mp4)
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├── calibrations/ # Per-session camera calibration (.toml) for 3D features
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├── calibrations.csv # InD calibration index
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├── calibrations_new.csv # OOD calibration index
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├── CollectedData_Cam-{A-F}.csv # InD 2D keypoint labels (x,y per keypoint)
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├── CollectedData_Cam-{A-F}_new.csv # OOD 2D keypoint labels
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├── config_fly-anipose.yaml # Sample Lightning Pose training config
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├── project.yaml # View and keypoint definitions (required by LP App)
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└── models/ # Pre-trained model checkpoints
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├── baseline/
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├── seed1/
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├── seed2/
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└── pleasant_ensemble/
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```
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See the Lightning Pose documentation for full details on the [multiview data directory structure](https://lightning-pose.readthedocs.io/en/latest/source/directory_structure_reference/multiview_structure.html) and [model directory structure](https://lightning-pose.readthedocs.io/en/latest/source/directory_structure_reference/model_dir_structure.html).
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## Usage with Lightning Pose
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The included `config_fly-anipose.yaml` is a ready-to-use training config. Key settings:
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- **Image resize:** 256 × 256
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- **Backbone:** `resnet50_animal_ap10k`
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- **Views:** Cam-A through Cam-F
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- **Keypoints:** 30
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Update `data.data_dir` and `data.video_dir` to absolute paths on your machine before training.
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```bash
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python scripts/train_hydra.py --config-path /path/to/fly_anipose_subset \
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--config-name config_fly-anipose
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```
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## Citation
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If you use this dataset, please cite the original Anipose paper:
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```bibtex
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@article{karashchuk2021anipose,
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title = {Anipose: A toolkit for robust markerless 3D pose estimation},
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author = {Karashchuk, Pierre and Rupp, Katie L and Dickinson, Evyn S and
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Walling-Bell, Sarah and Sanders, Elisha and Azim, Eiman and
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Brunton, Bingni W and Tuthill, John C},
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journal = {Cell Reports},
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volume = {36},
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number = {13},
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year = {2021},
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doi = {10.1016/j.celrep.2021.109730}
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}
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```
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Original data archive: https://doi.org/10.5061/dryad.nzs7h44s4
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