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src/task_metadata.json
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{
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"task_id": "circrna-discovery",
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"name": "Circular RNA Discovery: C. elegans Wild-type vs fust-1 Mutant",
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"description": "This task analyzes paired-end RNA-seq data to discover circular RNAs (circRNAs) in C. elegans. Four samples from two conditions (wild-type N2 and fust-1 mutant, 2 replicates each) are provided as paired-end FASTQ files, subset to chromosome I only. A reference genome (chrI.fa), gene annotation (chrI.gtf), and gene prediction file (chrI.txt) are included. The goal is to detect back-splice junctions characteristic of circular RNA molecules, quantify them across samples, and characterize the detected circRNAs. Data from nf-core/circrna test dataset (SRA SRX9313375-SRX9313380).",
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"task_prompt": "Detect circular RNAs from paired-end RNA-seq data of C. elegans chromosome I. Four paired-end FASTQ samples (2 wild-type N2, 2 fust-1 mutant) are in data/. A chromosome I reference genome, gene annotation (GTF), and gene prediction file are in reference/. Trim adapters, align reads to detect chimeric back-splice junctions, identify circular RNA candidates, quantify back-splice junction read counts, and characterize the detected circular RNAs by length and genomic distribution. The output should be a CSV file with columns: 'metric','value'.\n<example>metric,value\nsamples_analyzed,4\ntotal_circular_rnas,134\nshared_by_multiple_tools,0\ntotal_back_splice_reads,765\nmean_circrna_length,1852\nmin_circrna_length,192\nmax_circrna_length,15586\ndetection_tools_used,2\nchromosomes_with_circrnas,1\nn2_samples,2\nfust1_samples,2</example>",
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"download_urls": {
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"data": [
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"filename": "data.tar.gz",
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"url": "https://huggingface.co/datasets/lingzhi227/Extended-BioAgentBench/resolve/main/tasks/circrna-discovery/data.tar.gz"
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],
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"reference_data": [
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"filename": "reference.tar.gz",
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"url": "https://huggingface.co/datasets/lingzhi227/Extended-BioAgentBench/resolve/main/tasks/circrna-discovery/reference.tar.gz"
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}
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],
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"results": [
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{
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"filename": "results.tar.gz",
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"url": "https://huggingface.co/datasets/lingzhi227/Extended-BioAgentBench/resolve/main/tasks/circrna-discovery/results.tar.gz"
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}
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}
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