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+ # GSE208154 – Complete Cell Atlas of Aging C. elegans
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+
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+ ## 📄 Description
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+ This dataset provides a comprehensive single-cell RNA sequencing (scRNA-seq) profile of *C. elegans* across six adult time points. It is a highly curated developmental and aging atlas, useful for longevity, developmental biology, and transcriptomic studies.
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+
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+ ## 📊 Dataset Summary
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+ - **Species:** Caenorhabditis elegans
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+ - **Type:** Single-cell RNA-seq (scRNA-seq)
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+ - **Samples:** 11 GSM samples from GEO
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+ - **Format:**
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+ - `GSE208154_expression_sparse.parquet`: Sparse gene expression matrix
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+ - `GSE208154_metadata.parquet`: Cell metadata
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+
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+ ## 📁 Files Included
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+ - `GSE208154_expression_sparse.parquet`
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+ - `GSE208154_metadata.parquet`
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+
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+ ## 📥 How to Load
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+ ```python
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+ import scipy.sparse
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+ import pandas as pd
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+
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+ # Load expression matrix
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+ X = scipy.sparse.load_npz("GSE208154_expression_sparse.parquet")
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+
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+ # Load metadata
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+ metadata = pd.read_parquet("GSE208154_metadata.parquet")
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+ ```
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+
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+ ## 💡 Use Cases
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+ - Aging research in model organisms
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+ - Cell type classification and developmental biology
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+ - Benchmarking unsupervised and supervised learning on scRNA-seq data
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+ - Pseudotime or trajectory inference studies
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+
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+ ## 📚 Citation
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+ If you use this dataset, please cite:
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+ > Lipinszki, Z., et al. (2022). A complete single-cell transcriptomic atlas of aging *C. elegans*. *Nature Aging*, DOI: [10.1038/s43587-022-00217-x](https://doi.org/10.1038/s43587-022-00217-x)
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+
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+ ## 🙏 Acknowledgments
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+ We thank Calico Life Sciences for generating and sharing this valuable resource and NCBI GEO for providing public access.
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+
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+ Curated and converted by Iris Lee-participant of the Longevity Hackathon 2025. ### 🧑‍💻 Team: MultiModalMillenials. Iris Lee (`@iris8090`)