Input: The workup included skin tests (up to 1 microgram/ml, Pharmacia), measurement of specific serum IgE with RAST-CAP (Pharmacia), and CAST with three concentrations of bee (Apis mellifera) and wasp (Vespula spec.) venom (Aquagen ALK).

IgE


Input: The proliferation potential protein-related (P2P-R) gene with domains encoding heterogeneous nuclear ribonucleoprotein association and Rb1 binding shows repressed expression during terminal differentiation.

proliferation potential protein-related


Input: Recent application of recombinant canarypox ALVAC/HIV-1 vectors as vaccine immunogens in HIV-1,-noninfected volunteers has produced CTL responses in a significant number of vaccinees.


Input: The relevance of these elements in conferring anaerobic induction of gpc4 gene expression is discussed.

gpc4 gene


Input: Exons A1a and A1b are separated from each other by a 124-nucleotide intron.


Input: A MEK-specific inhibitor (PD098059) (Dudley, D.

MEK


Input: These data support the idea that the repression activity of NRSF/REST depends upon the species of promoter to which it is linked and upon the proteins that bind to those promoters.

NRSF

REST


Input: Enhanced period-peak analysis of electro-encephalograms using a fast sinc function.


Input: In summary, p56 (lck) activity stimulates G1/S transition in immature thymoblasts and maintains cell viability via transduction of constitutive activation signals downstream to components of the Ras/Raf/MAPK pathway.

lck

Ras

Raf

MAPK


Input: These findings suggest that intraarterial neoadjuvant chemotherapy should be effective on advanced cervical cancer.


Input: The FBF questionnaire did not highlight a characteristic basic symptoms profile of schizoaffective disorder, when compared with bipolar affective disorder and schizophrenia.


Input: The alternative sigma factor sigmaB in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock

sigma factor sigmaB in Staphylococcus aureus

sigB operon


Input: Cognate promoter elements implicated in glucocorticoid-and cAMP-mediated regulation as well as in liver-, myeloid-, and lymphocyte-specific expression are located within the 5' flanking sequence.


Input: We have studied the biokinetics of BLM labeled with indium-111 (In-111).


Input: CD3 cross-linking induced tyrosine phosphorylation of Sam68 in uninfected T cells.


Input: Acetoin-dependent expression of the acoABCD operon could be restored in the E. coli acoK mutants by supplying a plasmid carrying an intact acoK, suggesting a transactivating function of the gene product.

acoABCD operon

E. coli acoK mutants

acoK


Input: Mutations in the alpha and sigma-70 subunits of RNA polymerase affect expression of the mer operon.

alpha and sigma-70 subunits of RNA polymerase

mer operon


Input: Taken together, we provide evidence for the existence of an activator, NFE, which in combination with the p50 and c-Rel proteins, are part of the transcription factor machinery that regulates 3' enhancer activity, and thus the control of the IgH locus in late B lymphocyte development.

NFE

c-Rel proteins

IgH locus


Input: The neuroprotective efficacy of the selective N-type voltage-sensitive calcium channel blocker, SNX-111, was evaluated in spontaneously hypertensive rats subjected to 60 min of focal cerebral ischemia by permanent ligation of the right common carotid artery and temporary occlusion of the right middle cerebral artery.


Input: The genes encoding the transcription factor yTAFII60, the G4p1 protein and a putative glucose transporter are contained in a 12. 3 kb DNA fragment on the left arm of Saccharomyces cerevisiae chromosome VII.

transcription factor yTAFII60

G4p1 protein


Input: The protector doubled the estimated fall distance for fracture of the trochanter.


Input: Marinol/marijuana usage was associated with depressed CD4 + counts and elevated amylase levels within the DDI subgroup.

CD4 +

amylase


Input: METHODS: Fifty-eight patients who underwent a curative hepatic resection for HCC and whose preoperative AFP levels were > 100 ng/ml, were selected for this study.

AFP


Input: Calmodulin-binding peptide (CBP), a peptide of 26 amino acids derived from muscle myosin light chain kinase (MLCK), binds to calmodulin with nanomolar affinity.

Calmodulin-binding peptide

CBP

muscle myosin light chain kinase

MLCK

calmodulin


Input: Here we show that the type of tyrosine kinase receptor stimulated also participates in the nature of the cAMP effect.

tyrosine kinase receptor


Input: To gain insight into the possible involvement of p21 in liver cell growth, the expression and regulation of the p21 gene was evaluated in rodent models of liver regeneration and specimens of human liver diseases.

p21 gene


Input: Northern blot analysis of LeMT (A) and LeMT (B) showed that transcripts of both MT-like genes were more abundant in leaves than roots in tomato plants grown without addition of extra metal ions, a characteristic of type II MTs.

LeMT (A)

LeMT (B)

MT-like genes

type II MTs


Input: The HBP gene is composed of eight exons covering 19. 5 kb on the short arm of chromosome 4.

HBP gene


Input: In some instances, this is partly mediated by the expression of virally encoded proteases which lead to the cleavage of initiation factor eIF4G.

initiation factor eIF4G


Input: The best endometrial ablation was seen when SnET2 was given by intrauterine administration with light treatment at 150 J/cm 24 hours later.


Input: Unlike class II-and III-specific TBP-TAF complexes, the corresponding murine and human class I-specific transcription initiation factor TIF-IB/SL1 exhibits a pronounced selectivity for its homologous promoter.

TBP

TAF

TIF-IB


Input: We now describe the identification of DEK as this 43-kDa pets factor.

DEK

43-kDa pets factor


Input: These elements comprise a binding site for Krox proteins, one for nuclear factor 1, an octamer motif that binds POU-homeodomain proteins, and a novel TN control element.

Krox proteins

nuclear factor 1

POU-homeodomain proteins

TN control element


Input: Tissue samples were obtained either from rats that had been exposed to opiate withdrawal following a seven day morphine infusion or sham treated control subjects.


Input: Therefore, the impaired floor plate development in oep mutants is not caused by the absence of the floor plate inducer shh.

oep mutants

floor plate inducer shh


Input: RESULTS: The medians of average daily CD34 + cell yields for patients who received paclitaxel plus CY, CE, and CEP with G-CSF were 12. 9, 11. 03, and 5. 37 x 10 (6)/kg, respectively, compared with 2. 02 x 10 (6)/kg in the reference group that received CY with G-CSF (P = <. 0001,. 002, and. 09, respectively).

CD34 +

G-CSF

G-CSF


Input: Increased p53 activity induced by OP is not due to elevated p53 mRNA nor to protein levels.

p53 mRNA


Input: CONCLUSION: Chronic administration of misoprostol may have caused a negative natriuretic effect in cirrhotic patients with ascites.


Input: The intensity of the SSTEs is an order of magnitude larger than that of the MSEs and determines the signal contrast if both effects are selected simultaneously.


Input: In eukaryotic cells, premature termination of translation at nonsense codons has been implicated as the cause of a variety of posttranscriptional events, including rapid mRNA decay in the cytoplasm or the nucleus, altered splice site selection, and exon skipping.


Input: The region of plasmid pCM2 encoding the pathogenicity locus pat-1 was mapped by deletion analysis and complementation studies to a 1. 5-kb Bg/II/SmaI DNA fragment.

pathogenicity locus pat-1

Bg/II

SmaI


Input: Transient cotransfection of tat cDNA in sense orientation (tat/S), together with a plasmid containing the c-fos promoter (FC3, from-711 to + 42) in front of the bacterial chloramphenicol acetyltransferase (CAT) gene significantly enhanced CAT activity in Jurkat cells activated by the addition of 15% fetal calf serum (FCS) or 5 micrograms/mL phytohemagglutinin plus 10 (-7) mol/L phorbol myristate acetate (PMA) and U937 cells activated by 15% FCS or 10 (-7) mol/L PMA.

tat cDNA

tat/S

c-fos promoter

bacterial chloramphenicol acetyltransferase

CAT

CAT

phytohemagglutinin


Input: Snail mortality did not differ among the various treatment conditions.


Input: Exploratory-motor task to evaluate right frontal lobe damage.


Input: To avoid complications, accurate quantitative analysis of NO and NO2 is necessary during this therapy.


Input: Our results demonstrate that cis elements positioned between the alpha 3 and beta 4 coding regions are important for establishing part of the restricted CNS patterns of beta 4, alpha 3, and alpha 5 gene transcription.

alpha 3

beta 4

beta 4

alpha 3

alpha 5 gene


Input: RNase protection experiments confirmed the presence of the GHR1-279 variant in IM-9 cells and human liver.

RNase

GHR1-279 variant


Input: Functional tests using a reporter gene, containing Stat5-binding elements, confirmed that while the variant form was inactive by itself, it could inhibit the function of the full-length receptor.

Stat5-binding elements


Input: Conversely, activated glucocorticoid receptors suppressed the transactivation function of p53, while transrepression by p53 was largely unaffected.

glucocorticoid receptors


Input: This promoter segment could drive expression of a luciferase reporter gene, could confer correct transcriptional initiation to the reporter and could confer the EGF-responsiveness previously observed in the native gene.

luciferase reporter gene

EGF


Input: This is the first report that an in vitro-synthesized alphavirus RNA lacking a poly (A) tail can initiate infection and produce 3' polyadenylated viral genome in vivo.


Input: The human coreceptor was as active as the simian for SIV entry.


Input: RESULTS: Among women, the age-related decline in BMD at all non-spine skeletal sites was significantly different from zero, with the largest decline seen at the femoral neck (-. 0038 g/cm2/y, p <. 001) and the smallest at the trochanter of the hip (-. 0023 g/cm2/y, p =. 03).


Input: Sixty-nine (44%) patients were administered 250 MBq (7 mCi) 99Tcm-tetrofosmin at rest followed 4 h later by 750 MBq (21 mCi) during stress (the 1 day protocol), whereas 88 (56%) patients had rest and stress imaging studies on two separate days, receiving a 500 MBq (14 mCi) dose of 99Tcm-tetrofosmin on each occasion (the 2 day protocol).


Input: Myocardial SPET imaging with 99Tcm-tetrofosmin in clinical practice: comparison of a 1 day and a 2 day imaging protocol.


Input: 99Tcm-DMP-HSA showed an almost identical behaviour to in vitro labelled red blood cells (RBCs), which are generally considered the reference standard for blood pool agents.


Input: Relief from autoinhibition and a subsequent 10-60-fold increase in V (max) have been observed upon N-SH2 domain engagement by a specific phosphotyrosyl ligand or upon deletion of the SH2 domains to yield the catalytic PTPase domain.

N-SH2 domain

SH2 domains

PTPase domain


Input: We have thus identified the first mammalian homolog of yeast UPF1, a protein that regulates levels of nonsense mRNA, and we tentatively name this protein human HUPF1 (for human homolog of UPF1).

mammalian homolog of yeast UPF1

human HUPF1

human homolog of UPF1


Input: A miracle cure and its solution


Input: The addition of zidovudine did not influence this transfer.


Input: DNA strand exchange catalyzed by Rad51 protein is also greatly stimulated by RPA.

Rad51 protein

RPA


Input: Ume6p, which also controls the expression of early meiotic genes, represses CAR1 expression through a sequence called URS, as a function of nitrogen availability.


Input: The second class of cDNA hybridized to a 13 kb transcript, which was approximately twice as large as the mammalian lactase mRNA.

mammalian lactase mRNA


Input: In cell lines transformed by BCR/ABL, CRKL was tyrosine phosphorylated, while CRK was not.

BCR

ABL

CRKL

CRK


Input: In untransformed cells, three major proteins coprecipitated with CRKL, identified as C3G, SOS and c-ABL.

CRKL

SOS

c-ABL


Input: RESULTS: Nodular opacities, mainly centrilobular in distribution, were the most common finding, seen in 21 (72%) and 15 (65%) of patients with MTB and NTMB, respectively.


Input: Circulating oxytocin in male guinea pigs affected by the female cohabitation and reproductive condition.

oxytocin


Input: Antisense transcription of a murine FGFR-3 psuedogene during fetal developement.

murine FGFR-3 psuedogene


Input: A previous report described that a 548-bp BglII-SmaI fragment has an SSO activity (ori2; Kataoka et al., Mol.

BglII

SmaI


Input: The human CD38 gene consists of 8 exons that extend more than 77 kb on the human genome.

human CD38 gene


Input: Type IV splice pattern, containing exon U3 and S was found both in kidney and ovary.


Input: GH deficiency may be absolute, but often is not and the diagnosis may be complicated by a constellation of physical and hormonal findings that are along a spectrum from low normal GH sufficiency to absent GH secretion.

GH

GH

GH


Input: Positive CRP test were seen.

CRP


Input: During the febrile period, pleocytosis was associated with high levels of IgG, IL-6, TNF-alpha, and PGE2 in the cerebrospinal fluid.

IgG

TNF-alpha


Input: Recognition mechanisms of the minus-strand origin of phage f1 by Escherichia coli RNA polymerase.

Escherichia coli RNA polymerase


Input: The minimum and essential subunits for RNA synthesis have not yet been identified.


Input: Syndecan-4 is a focal adhesion component, and this interaction may both localize PKC and amplify its activity at sites of forming adhesions.

PKC


Input: The 3ASubE cells expressing CXCR2 with mutation of Ser-346,-347, and-348 to alanine, or with mutation of only one serine in this domain, continue to be phosphorylated in response to ligand and are 60-70% desensitized following the initial ligand challenge.


Input: Members of the MAPK family include the extracellular response kinases (ERKs or p42/44 (MAPK)), the c-Jun amino-terminal kinases (JNKs), and the p38/Hog 1 protein kinases.

MAPK family

extracellular response kinases

ERKs

MAPK

c-Jun amino-terminal kinases

JNKs

Hog 1


Input: MOP5 contained the characteristic PAS domain and a variable C terminus; it is possible that the cDNA contains a bHLH domain, but the entire open reading frame has yet to be completed.

PAS


Input: Some 8. 8 kb of the Lactobacillus sake plasmid pCIM1 was sequenced, revealing eight tightly clustered open reading frames (ORFs) downstream from lasA, which encodes pre-lactocin S.

lasA

pre-lactocin S


Input: Purified Pra was also shown to physically interact with pyruvate kinase (Pk); Pk and Pra can form a complex, but when the 12-kDa Ndk, Pk, and Pra are all present, Pk has a higher affinity than Pra for forming a complex with the 12-kDa Ndk.

Purified Pra

pyruvate kinase

Pk

Pk

Pra

12-kDa Ndk

Pk

Pra

Pk

Pra

12-kDa Ndk


Input: Molecular characterization of a large Borrelia burgdorferi motility operon which is initiated by a consensus sigma70 promoter.

sigma70 promoter


Input: M. leprae OxyR was overproduced and purified, and its binding to the oxyR-ahpC intergenic region of M. leprae was demonstrated.

M. leprae OxyR

oxyR

ahpC


Input: The binding site for OxyR overlapped P1oxyR, reminiscent of the autoregulatory loops controlling expression of oxyR in enteric bacteria and characteristic of the LysR superfamily in general.

OxyR

oxyR

LysR superfamily


Input: Thirty-five patients were included.


Input: Grade 3-4 mucositis was present in 13% of the cycles.


Input: Therefore more active and tolerable salvage regimens are needed.


Input: None of these organic manifestations was discovered by scintigraphic means.


Input: While TGF-beta1 does not alter the mitogen-induced expression and abundance of G1 phase cyclin D1 and cdks 4 and 2 in MCs, it inhibits cyclin E-cdk 2 activity, thus preventing mitogen-elicited phosphorylation and inactivation of pRb in G1 phase and transition to S phase.

cyclin D1

cdks 4 and 2

cyclin E

cdk 2

pRb


Input: However, the range of values observed is suggestive of the need to investigate districts with contrasting values of SYPLR with respect to the inter-relationships between sociodemographic characteristics, duration of symptoms, clinical presentation and treatment efficacy.


Input: The prominent lesions were confined to the cerebral surface layer and leptomeningeal tissue including the arachnoid vessels, which were all bathed in the cerebrospinal fluid, suggesting that some necrotizing toxins had been secreted into the fluid by the B. cereus.


Input: This fragment contained the C-terminal 47 nucleotides of leuB, encoding 3-isopropylmalate dehydrogenase; asd, encoding aspartate-beta-semialdehyde dehydrogenase (Asd); and orfA, whose product showed similarity to the Asd proteins from Vibrio spp.

leuB

3-isopropylmalate dehydrogenase

asd

aspartate-beta-semialdehyde dehydrogenase

Asd

Asd proteins from Vibrio spp


Input: Hovenitin I and (+)-ampelopsin, both of which were principal ingredients of the active fractions from this natural medicine, were found to show an inhibitory activity on the ethanol-induced muscle relaxation in rats.


Input: Purified P7 could be assembled onto particles lacking P7 and particles lacking both P2 (RNA polymerase) and P7.

Purified P7

RNA polymerase


Input: However, the same U1A peptide, when conjugated to BSA, inhibits vertebrate PAP.

U1A peptide

BSA

vertebrate PAP


Input: Plasma levels of protein C, protein S, and antithrombin III in patients with subarachnoid haemorrhage.

protein C

protein S

antithrombin III


Input: Idiopathic bilateral recurrent branch retinal arterial occlusion (IBRBRAO) is a rare syndrome characterized by migraine headaches, tinnitus, vertigo, hearing loss, and recurrent branch retinal artery occlusion of unknown etiology.


Input: Epithelial and corneal thickness measurements by in vivo confocal microscopy through focusing (CMTF).


Input: Sequences downstream of the two human CA genes, possibly containing homologous enhancer elements, have not yet been reported.

human CA genes


Input: Recombinant plasmids containing the entire 322 nt 5' UTR of exon 1 and a 1630 nt segment of 5' flanking sequence stimulated luciferase activity nearly 70 times higher than a promoterless control plasmid.

luciferase


Input: Analysis for DNA-protein interactions by in vitro DNase-I footprinting identified a broad region of protection extending from nt-12 to + 38.

DNase-I


Input: It is also homologous to other sugar carriers from human, mouse and Escherichia coli.


Input: Until now, each of these loci was considered to contain three genes (for regulator, permease and alpha-glucosidase), but a fourth gene, presumably an extra alpha-glucosidase gene, was found at MAL1 adjacent to the usual cluster of three genes.

regulator

permease

alpha-glucosidase

alpha-glucosidase gene


Input: Autorosette-forming cells are characterized by a high activity of alkaline and acid phosphatases and low NBT-test values.

alkaline and acid phosphatases


Input: Analysis of homologous DNA sequences within the first intron of the mouse and human mts1 genes: kB-like site and microsatellite DNA

mouse and human mts1 genes

kB-like site


Input: The integration at the VIP CyRE of the Jak-Stat and AP-1 signaling pathways with other pre-existing proteins provides a cellular mechanism for cell-and cytokine-specific signaling.

VIP CyRE

Jak

Stat


Input: These studies point to the involvement of the MAP kinase pathway in the activation of monocytic cells during transmigration to inflammatory sites.

MAP kinase


Input: Our results show that the temperature-sensitive rad54-3 allele blocks mitotic recombination between tandemly repeated DYZ3 satellite sequences and significantly stabilizes a human DYZ5 satellite-containing YAC clone.

temperature-sensitive rad54-3 allele

DYZ3 satellite sequences

human DYZ5


Input: A P22 R17 derivative with an OcRNA site (P22 R17 [A (-10) U]) develops lytically following infection of these strains.


Input: Circularized Ac/Ds transposons: formation, structure and fate.

Ac

Ds


Input: In this study, we have examined the regulation of B-Myb activity by cyclin-Cdks.

B-Myb

cyclin

Cdks


Input: Candidate tumor suppressor genes, Mts1 (p16INK4a) and Mts2 (p15INK4b), have been mapped to this region, but by Southern blot analysis, no homozygous deletions were detected in either gene.


Input: Excision repair at the level of the nucleotide in the Saccharomyces cerevisiae MFA2 gene: mapping of where enhanced repair in the transcribed strand begins or ends and identification of only a partial rad16 requisite for repairing upstream control sequences.

Saccharomyces cerevisiae MFA2 gene


Input: This locus maps approximately 160 kbp from the genes encoding cytadherence-associated proteins HMW1 and HMW3, and yet insertions therein result in loss of these proteins and a hemadsorption-negative (HA-) phenotype, prompting the designation cytadherence-regulatory locus (crl).

cytadherence-regulatory locus

crl


Input: Coliphage 186 B is a 72-amino acid protein belonging to the Ogr family of analogous transcription factors present in P2-like phage, which contain a Cys-X2-Cys-X22-Cys-X4-Cys presumptive zinc-finger motif.

Coliphage 186 B

Ogr


Input: The UV absorption spectrum revealed a shoulder at 250 nm, characteristic of CysS-Cd (II) ligand-to-metal charge-transfer transitions, and the difference absorption coefficient after acidification (delta epsilon 248, 24 mM-1 cm-1) indicated the presence of a Cd (Cys-S) 4 center.


Input: Diacylglycerol kinase (DGK) attenuates levels of second messenger diacylglycerol in cells and produces another (putative) messenger, phosphatidic acid.

Diacylglycerol kinase

DGK


Input: Recently, we have reported the cloning of the germ cell-specific, nuclear orphan receptor germ cell nuclear factor (GCNF)/RTR.

germ cell nuclear factor

GCNF

RTR


Input: Based on the sequence of the conRTRE, we located a putative RTRE, referred to as P2-RE, in the 5' promoter-flanking region of the mouse protamine 2 gene, which is induced during the same stage of spermatogenesis as RTR.

conRTRE

RTRE

mouse protamine 2 gene

RTR


Input: Active complexes eluted at a higher molecular weight than inactive complexes, were relatively deficient in both p21 and p27, and contained Cdk2 with increased threonine 160 phosphorylation, consistent with a mechanism of activation of cyclin E-Cdk2 involving both reduced CDK inhibitor association and CDK-activating kinase-mediated phosphorylation of Cdk2.

cyclin E

CDK

CDK-activating kinase


Input: Thirty Class I and Class II recessions in 30 patients were treated with a modified subepithelial connective tissue graft procedure.


Input: The derivatives of the latter two cell lines showed increased expression of the p27Kip1 protein and inhibition of cell growth.

p27Kip1 protein


Input: The perioperative mortality of all patients was 1. 9%, the mortality of patients older than 70 years was 4. 3%.


Input: Chronic renal failure patients essentially die from cardiovascular causes, and the frequency of malignant disease responsible for death is estimated to be 10%.


Input: The new FIGO definition of cervical cancer stage IA: a critique.


Input: The effect of Vpu on class I biogenesis was analyzed in more detail using a Vpu-expressing recombinant vaccinia virus (VV).

Vpu

Vpu


Input: Inductively coupled plasma atomic emission spectroscopy was employed to obtain the tissue silicon measurements.


Input: An egg protein, lysozyme, is a still unlabeled additive currently used in cheese preparation.

lysozyme


Input: Similarly, the DNA-binding activity of activator protein 1 (AP-1) is modified by a DNA repair enzyme, redox factor 1 (Ref-1), which is identical to a DNA repair enzyme, AP endonuclease.

activator protein 1

DNA repair enzyme

redox factor 1

DNA repair enzyme

AP endonuclease


Input: Having an LRR domain and an SH3-binding domain, Acan125 and the C. elegans homologue define a novel family of bifunctional binding proteins.

LRR domain

SH3-binding domain


Input: However, its participation in gagging induced by oropharyngeal irritation is unclear.


Input: The capacity to repair 8-OxoG has been measured in cell-free extracts of wild-type and ogg1 strains using a 34mer DNA fragment containing a single 8-OxoG residue paired with a cytosine (8-OxoG/C) as a substrate.


Input: Results from our and other laboratories have suggested that UCN-01 induces preferential G1-phase accumulation in several human tumor cell lines tested.


Input: Northern and RT-PCR analysis of Ube3a expression in mouse tissues from animals with segmental, paternal uniparental disomy failed to detect substantially reduced or absent expression compared to control animals, failing to provide any evidence for maternal-specific expression from this locus.


Input: Two predominant effects were noted: (i) the Xaa residue in the dual phosphorylation motif Thr-Xaa-Tyr as well as the length of L12 influence p38 substrate specificity, and (ii) the length of L12 plays a major role in controlling autophosphorylation.


Input: A novel, testis-specific mRNA transcript encoding an NH2-terminal truncated nitric-oxide synthase. mRNA diversity represents a major theme of neuronal nitric-oxide synthase (nNOS) gene expression in somatic cells/tissues.

testis-specific mRNA transcript

NH2-terminal truncated nitric-oxide synthase

neuronal nitric-oxide synthase

nNOS


Input: Unlike the MAR-binding domain, the homeodomain when isolated binds poorly and with low specificity to DNA.

homeodomain


Input: Cyclin D1 could not be displaced from cdk4 in the resistant 184A1L5R cell lysates.

Cyclin D1


Input: The importance of posttranslational regulation of p15INK4B by TGF-beta is underlined by the observation that in TGF-beta-resistant 184A1L5R, although the p15 transcript increased, p15INK4B protein was not stabilized and did not accumulate, and cyclin D1-cdk association and kinase activation were not inhibited.

TGF-beta

TGF-beta

p15 transcript

p15INK4B protein

cyclin D1

cdk


Input: To identify cis-acting elements that target c-myc mRNA for downregulation during myogenesis, we stably transfected C2C12 cells with mutant myc genes or chimeric genes in which various myc sequences were fused to the human beta-globin gene or to the bacterial chloramphenicol acetyltransferase (CAT) gene.

c-myc mRNA

mutant myc genes

myc sequences

human beta-globin gene

bacterial chloramphenicol acetyltransferase

CAT


Input: Furthermore, experiments with 32D temperature-sensitive p53 cells indicate that aberrant tal-1 expression at the permissive temperature does not exert a proliferative effect but causes p53-mediated apoptosis, i. e., the tal-1 proliferative effect depends on the integrity of the cell cycle checkpoints of the host cell, as observed for c-myc and other oncogenes. tal-1 mutant experiments indicate that ectopic tal-1 effects are mediated by both the DNA-binding and the heterodimerization domains, while the N-terminally truncated tal-1 variant (M3) expressed in T-ALL malignant cells mimics the effects of the wild-type protein.

c-myc

tal-1 mutant

N-terminally truncated tal-1 variant


Input: DNA-binding activity was not increased by the addition of forskolin to thecal or luteal cells.


Input: However, in some of them either pattern may predominate or be exclusively present.


Input: Upon induction of SOS, viability increased 2-6-fold.


Input: In particular, Western, supershift, and promoter deletion analyses suggested a role for CCAAT/enhancer-binding protein-beta (C/EBP-beta) binding site between-2010 and-1954 in regulating transcription of collagenase-1 in monocytic cells.

CCAAT/enhancer-binding protein-beta

C/EBP-beta


Input: 11-Aminoundecanoyl-SK-NH2 and 11-aminoundecanoyl-SH-NH2 establish that a simple alkyl backbone can maintain an appropriate distance between three elements critical for recognition by the fungal enzyme' s peptide-binding site: a simple omega-terminal amino group, a beta-hydroxyl, and an epsilon-amino group or an imidazole.


Input: Cdc2 co-precipitates with Pch1 in S. pombe cell lysates, although Cdc2 may not be the major catalytic partner of a Pch1 kinase in vivo.

Pch1 kinase


Input: Little is known about the mechanism, but the availability of rapid facile assays for monitoring immunoglobulin hypermutation would greatly aid the development of culture systems for hypermutating B cells as well as the screening for individuals deficient in the process.

immunoglobulin


Input: Expression of the human heat shock protein 70 gene (hsp70) is induced by various kinds of stress and by oncogenes.

human heat shock protein 70 gene


Input: Of these proteins, five have previously been shown to be phosphorylated during mitosis (epithelial-microtubule associated protein-115, Oct91, Elongation factor 1gamma, BRG1 and Ribosomal protein L18A), five are related to proteins postulated to have roles in mitosis (epithelial-microtubule associated protein-115, Schizosaccharomyces pombe Cdc5, innercentrosome protein, BRG1 and the RNA helicase WM6), and nine are related to transcription factors (BRG1, negative co-factor 2alpha, Oct91, S. pombe Cdc5, HoxD1, Sox3, Vent2, and two isoforms of Xbr1b).

epithelial-microtubule associated protein-115

Elongation factor 1gamma

Ribosomal protein L18A

epithelial-microtubule associated protein-115

Schizosaccharomyces pombe Cdc5

innercentrosome protein

RNA helicase WM6

negative co-factor 2alpha


Input: Thus, depending on their location, psoralen cross-links affected different steps in the initiation process.


Input: Activation of alpha 4 beta 1 with TS2/16 inhibited the cytoplasmic protrusions and cell migration but did not affect the pattern of phosphorylation.

alpha 4 beta 1


Input: Performance on two verbal measures-the National Adult Reading Test-Revised (NART-R) and the Vocabulary subtest from the Wechsler Adult Intelligence Scale-Revised (WAIS-R)-showed strong correlations with level of education.


Input: L-canavanine, a selective inhibitor of the inducible nitric oxide synthase, increases the mean systemic filling pressure, thereby improving venous return, under these conditions.

nitric oxide synthase


Input: RESULTS: Of the 29 patients who received concurrent chemotherapy and G-CSF, ten (34%; 95% confidence interval [CI], 17. 9 to 54. 3%) were believed to have clinically significant bleomycin toxicity.

G-CSF


Input: The exonic sequence encodes a protein of 495 amino acids that is nearly identical to the previously reported protein sequence of human GPT-1.

human GPT-1


Input: Here we characterize an S. cerevisiae Swi3 homolog (Swh3) and present evidence that it associates in a complex with a Snf2 homolog, Sthl.

S. cerevisiae Swi3 homolog

Snf2 homolog

Sthl


Input: Shc stimulates Ras/mitogen-activated protein kinase (MAPK) through forming a complex with Grb2 at the phosphorylated tyrosine (Y) residue 317.

Shc

Ras

mitogen-activated protein kinase

MAPK


Input: In contrast, Y239/240F Shc, but not Y317F Shc, reduced the EGF-induced c-myc message.

Y239/240F Shc

Y317F Shc

EGF

c-myc


Input: The Ras guanine nucleotide-binding protein functions as a molecular switch in signalling downstream of protein-tyrosine kinases.

Ras guanine nucleotide-binding protein

protein-tyrosine kinases


Input: We show that Rlm1 and Smp1 have MEF2-related DNA-binding specificities: Rlm1 binds with the same specificity as MEF2, CTA (T/A) 4TAG, while SMP1 binds a more extended consensus sequence, ACTACTA (T/A) 4TAG.

CTA (T/A) 4TAG


Input: Domains I and II of B " are buried upon assembly of the TFIIIB-DNA complex, as determined by protein footprinting.

B "

TFIIIB-DNA complex


Input: Domain switch experiments reveal that C/EBP beta proteins containing either the leucine zipper or the activation domain of C/EBP alpha are unable to stimulate the 2D5 promoter yet are fully capable of transactivating an artificial promoter bearing a high-affinity C/EBP site.

C/EBP beta proteins

C/EBP alpha

2D5 promoter

C/EBP site


Input: PRH75, a new nucleus-localized member of the DEAD-box protein family from higher plants.

DEAD-box protein family


Input: These activities are all required for stimulation of cell growth by middle-T and activate members of the MAP kinase family.

middle-T

MAP kinase family


Input: A role for the small GTPase Rac in polyomavirus middle-T antigen-mediated activation of the serum response element and in cell transformation.

small GTPase Rac

polyomavirus middle-T antigen


Input: Time-activity curves from the gastric region of interest were used, after subjection to appropriate corrective procedures, to calculate the mean gastric transit time (MTT 90) and the fraction of the test meal retained in the stomach after 90 min (F90).


Input: This study provides a direct demonstration of a role for G betagamma in mediating the agonist-stimulated translocation of GRK2 and GRK3 in an intact cellular system and demonstrates isoform specificity in the interaction of these components.

G betagamma


Input: Our results indicate that RA-mediated repression of the hMGP gene is due to binding of liganded RAR/RXR to a novel negative RA response element.

hMGP gene

RAR

RXR


Input: Point mutations of the five tyrosine residues in the cytoplasmic domain of the receptor were subsequently used to confirm our conclusions.


Input: We describe a novel method using Saccharomyces cerevisiae for detecting protein-truncating mutations in any gene of interest.


Input: Hepatocellular injury during preservation of human livers with UW and HTK solution.


Input: The electromyographically recorded responses consisted of an early R1 response in the orbicularis oculi muscle ipsilateral to the side of stimulation, a bilateral late response (ipsilateral R2 and contralateral Rc) and a third, R3 response, in the ipsilateral orbicularis oculi muscle.


Input: The prognosis of seven patients who received ABSCT was significantly better than that of 13 patients who received conventional therapy alone.


Input: (1994, Hum.


Input: The pigment intensity of both melanized colonies and appressoria of CAL transformants was weaker than that of the wild type.


Input: Malonate decarboxylation in Malonomonas rubra involves the formation of malonyl-S-[acyl-carrier protein] from acetyl-S-[acyl-carrier protein] and malonate, carboxyltransfer to a biotin protein and its decarboxylation that is coupled to delta mu Na + generation.

malonyl-S-[acyl-carrier protein]

acetyl-S-[acyl-carrier protein]

biotin protein


Input: The Jem peptide sequence shows a' leucine-zipper' dimerisation motif with limited homology to Fos/Jun and ATF/CREB proteins and several putative phosphorylation sites.

Jem

Fos

Jun

ATF

CREB


Input: Here we report the cloning of the protein that binds to enhancer site III.


Input: Further support for a direct interaction of Tub4p, Spc98p and Spc97p comes from the toxicity of strong SPC97 overexpression which is suppressed by co-overexpression of TUB4 or SPC98.


Input: Sequences needed for iron-regulated expression of sid1 were localized to a 306 bp region mapping 2. 3 and 2. 6 kb upstream of the ATG.


Input: Comparison of the p50 sequence to other cloned proteins revealed 89% homology with a glycosaminoglycan-binding protein and 54% homology with Drosophila cell cycle control protein (cdc) 37.

p50 sequence

Drosophila cell cycle control protein (cdc)


Input: Additional information including echocardiographic sequences, perioperative video sequences, x-ray analysis, angiograms, etc. is represented in the program.


Input: We report here that constitutively active Mek1 could activate p96h2bk in the absence of oncogenic Ras.

oncogenic Ras


Input: In Schizosaccharomyces pombe, the activity of the M-phase-inducing Cdc2/Cdc13 cyclin-dependent kinase is inhibited by Wee1 and Mik1 tyrosine kinases, and activated by Cdc25 and Pyp3 tyrosine phosphatases.

Mik1 tyrosine kinases

Pyp3 tyrosine phosphatases


Input: The sequencing of Stellate copies located along the discontinuous cluster revealed a complex pattern of diversification.

Stellate


Input: The results indicate that DNA methylation, chromatin structure, and transactivation at an Sp1 site contribute to the highly restricted expression of this myelomonocytic lineage specific gene.

Sp1 site


Input: In gel mobility shift assays, factors present in nuclear extracts derived from differentiated osteoblast bound to oligonucleotide probes containing the E-box 1 and E-box 2 elements.


Input: Laboratory exam: IDR of the tuberculin was high positive, chest radiography shows hilar bilateral calcifications, ORL exam shows a tumor at the foot of the epiglottis and anatomopathological exam shows low differentiated epidermoid carcinoma.

tuberculin


Input: Finally, we show that PhLP complexes, at least partially, with Gbetagamma in vivo.

PhLP

Gbetagamma


Input: CTF1alpha, expressed in Escherichia coli, showed specific binding to the palindrome 2 DNA fragment but not to palindrome 1 or mutant palindrome 2 DNA fragments, suggesting specific binding of CTF1alpha to palindrome 2.


Input: Cyclin G2 is highly expressed in the immune system where immunologic tolerance subjects self-reactive lymphocytes to negative selection and clonal deletion via apoptosis.

Cyclin G2


Input: A sterol-regulated protease initiates release of the NH2-terminal segments of sterol regulatory element-binding proteins (SREBPs) from cell membranes, thereby allowing them to enter the nucleus and to stimulate transcription of genes involved in the uptake and synthesis of cholesterol and fatty acids.

sterol regulatory element-binding proteins

SREBPs


Input: Thus, the P. aeruginosa orfX and vfr promoters are arranged in a back-to-back orientation rather than the face-to-face orientation of the dorf and crp promoters.

vfr promoters

crp promoters


Input: Sequence analysis of cloned PCR products confirmed the presence of two different nifV-like DNA fragments, which were subsequently used as nifV-and leuA-specific probes, respectively, to clone XbaI fragments of 2. 1 kbp (pOST4) and 2. 6 kbp (pOST2).

nifV-like DNA fragments

nifV

leuA-specific probes

XbaI fragments


Input: In contrast, the Anabaena strain 7120 leuA gene did not complement the nifV mutation of R229I efficiently.

leuA

nifV mutation


Input: (3) This effect occurs without removing TRs from the TRE.

TRs


Input: In a model of the ternary complex, the segment of NFAT nearest AP-1 is the Rel insert region (RIR), a feature that is notable for its hypervariability in size and in sequence amongst members of the Rel transcription factor family.

NFAT

Rel

Rel transcription factor family


Input: We thus conclude that the NFAT RIR plays an essential dual role in DNA recognition and cooperative binding to AP-1 family transcription factors.

NFAT RIR

AP-1 family transcription factors


Input: Thus, the association of PS1 fragments may be maintained during cycles of phosphorylation/dephosphorylation of the PS1 CTF.

PS1 fragments

PS1 CTF


Input: This protein can bind to a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb1 * 3, which is necessary for its regulation by phytochrome.

Arabidopsis light-harvesting chlorophyll a/b protein gene

Lhcb1 * 3

phytochrome


Input: A patient of Group B had severe athetoid CP with spasticity, being unable to right his trunk and neck.


Input: Dystonic movement of the left upper limb in a case of the right pontine hemorrhage


Input: It has repeatedly been shown that HCMV IE1/IE2 can independently transactivate HIV-1 LTR.

HIV-1 LTR


Input: TAR and Sp1-independent transactivation of HIV long terminal repeat by the Tat protein in the presence of human cytomegalovirus IE1/IE2.

TAR

HIV long terminal repeat

Tat protein


Input: Respiratory chain enzyme activity was normal.


Input: Cellular Csk was associated with several phosphoproteins, some of which were interacting with the Csk SH2 domain.

Cellular Csk

Csk SH2 domain


Input: Thus, our studies suggest that pV-mediated activation of HIV-1 LTR activity is controlled by the nuclear translocation of the NF-kappaB transcription factor, which is mediated by IkappaBalpha serine phosphorylation and degradation, but also by a still undefined NF-kappaB-independent pathway.

HIV-1 LTR

NF-kappaB transcription factor

IkappaBalpha

NF-kappaB


Input: FN-stimulated c-Src PTK activity was enhanced by wild type FAK expression, whereas FN-stimulated activation of ERK2 was blocked by expression of the c-Src binding site Phe-397 mutant of FAK.

FN

c-Src PTK

FAK

FN

c-Src binding site Phe-397 mutant

FAK


Input: A promising new cement, 4-META/MMA-TBB opaque resin, has shown remarkable adhesive properties as a bone cement in vivo.


Input: Each sample was analysed as soon as possible, and repeated 15, 30, 60 and 120 min after sampling.


Input: Like the elicitors of the hypersensitive reaction (HR) produced by E. chrysanthemi (HarpinEch) and E. amylovora (HarpinEa), the deduced 36-kDa protein does not possess a typical signal sequence, but it contains a putative membrane-spanning domain.


Input: Molecular characterization and expression of the Erwinia carotovora hrpNEcc gene, which encodes an elicitor of the hypersensitive reaction.

Erwinia carotovora hrpNEcc gene


Input: The mRNA from this gene, termed HES1, is ubiquitously expressed, but strongly so in heart and skeletal muscle.


Input: Anesthesia was maintained with isoflurane (ISO)/N2O/O2 inhalation.


Input: They observed depressed protein C activity that significantly (p < 0. 005) increased and became normal immediately after hemodialysis while factor X and factor VII increased (p < 0. 01; p < 0. 05) despite heparinization together with amount of serum lipoprotein (a).

protein C

factor X

factor VII

serum lipoprotein (a)


Input: We present a case of type II hyperbetalipoproteinemia in a patient whose diagnosis had been previously unrecognized, and who had previously been misdiagnosed with rheumatoid arthritis and later gout.


Input: These data provide evidence that the 2. 0-kb LAT is an intron of the mLAT pre-mRNA with a unique branch point.

2. 0-kb LAT

mLAT pre-mRNA


Input: The altered amino acid residues of the seven mutant 9ORF1 polypeptides clustered within three separate regions referred to as region I (residues 34 to 41), region II (residues 89 to 91), and C-terminal region III (residues 122 to 125).

mutant 9ORF1 polypeptides


Input: Rep-Rep protein interaction was confirmed in vitro through coimmunoprecipitation experiments with a bacterially expressed maltose-binding protein-Rep78 fusion protein in combination with [35S] methionine-labeled Rep78 synthesized in a coupled in vitro transcription-translation system.

Rep

Rep

maltose-binding protein


Input: Fourteen patients with New York Heart Association class II congestive heart failure were enrolled in a double-blind, cross-over study.


Input: The main advantages of Multi-MUP analysis are: (1) quick acquisition of many MUPs; (2) simultaneous collection of several MUPs at one recording site; (3) possibility to analyze not only low threshold MUPs; (4) less bias in the selection of MUPs and (5) the reproducibility of the results that allow the same reference values to be used in different laboratories.


Input: In several studies the RDFS has shown evidence of reliability and validity.


Input: These results suggest that dietary safflower phospholipids may be a valuable ingredient to layers for reducing liver triglycerides and serum cholesterol without any adverse effects.


Input: Using various techniques, we have undertaken a systematic analysis of the natural TATA-less human DNA polymerase beta (beta-pol) gene promoter.

human DNA polymerase beta

beta-pol


Input: Saturable spermidine transport in stk2:: lacZ mutants had an approximately fivefold-lower affinity and twofold-lower Vmax than in the parental strain.

lacZ mutants


Input: Inhibition of the Raf-1 kinase by cyclic AMP agonists causes apoptosis of v-abl-transformed cells.

Raf-1 kinase

v-abl


Input: Previous studies have indicated that a hamster cell line (ts13) with a point mutation in the TAF (II) 250/CCG1 (TAF (II) 250) gene shows temperature-sensitive expression of a subset of genes and arrests in late G1 at 39. 5 degrees C.

TAF (II) 250

TAF (II) 250


Input: We show here that the protein kinase MEKK1 can induce reporter gene expression from the atrial natriuretic factor (ANF) promoter, a genetic marker that is activated during in vivo hypertrophy.

atrial natriuretic factor

ANF


Input: Overexpression of EFG1 in C. albicans leads to enhanced filamentous growth in the form of extended pseudohyphae in liquid and on solid media.


Input: To determine if signing, when established, would compete with SIB when both were reinforced, extinction was then withdrawn.


Input: The husband in one of the married couples was treated for hepatitis of unidentified etiology in an Infectology Department four years ago.


Input: Both quantitative and qualitative analysis of individual cytoarchitectonic peculiarities of Meynart' s nucleus as well as of external part of dorsomedial nucleus of thalamus was performed in mentally normal individuals.


Input: Positional cloning has already produced the sequences of more than 70 human genes associated with specific diseases.


Input: The 30-day mortality in the CPB group and the non-CPB group were 20% and 4. 6%, respectively which was not statistically significant (p = 0. 06).


Input: As opposed to in vitro co-precipitation studies, the yeast two-hybrid screen reveals in vivo protein-protein interactions.


Input: This association appears to be mediated by Src-SH2 domain, because PECAM-1 can be precipitated by a GST-Src-SH2 affinity matrix.

Src

GST

Src


Input: MKK3 autophosphorylation and activation of p38 was also observed following coexpression of MKK3 with MEKK3, but not with MEKK2.


Input: An alternatively spliced MAdCAM-1 variant was identified that lacks exon 4 encoding the mucin domain, and may mediate leukocyte adhesion to LPAM-1 without adhesion to the alternate receptor, L-selectin.

mucin

L-selectin


Input: In summary, the data establish that the previously reported human MAdCAM-1 cDNA does indeed encode the human homologue of mouse MAdCAM-1, despite gross dissimilarities in the MAdCAM-1 C-terminal structures.

human MAdCAM-1 cDNA

mouse MAdCAM-1


Input: We have used a site-directed mutagenesis approach to examine how the Xaa and hydroxy (Ser/Thr) amino acid residues in sequons influence core-glycosylation efficiency.


Input: MATERIAL AND METHODS: The authors analyzed 41 persons formerly submitted to surgery (after 8 years and 4 months, as a mean), 31 to highly selective vagotomy, and 10 to truncal or selective vagotomy plus gastroduodenal drainage.


Input: This region does not contain an identifiable TATA element, indicating that transcription of the glutaminase gene is driven by a TATA-less promoter.

glutaminase gene


Input: Serum alkaline phosphatase activity, bone mass measurements, dual energy x-ray absortiometric analysis of mineral density, and mechanical testing values in vertebrae and femora of the-D Sal animals did not significantly differ from those in + D Sal animals.

Serum alkaline phosphatase


Input: The monkey LHR cDNA displayed 83-94% overall sequence homology with the other mammalian LHR cDNAs.

monkey LHR cDNA

mammalian LHR cDNAs


Input: In conclusion, marmoset monkey LHR seems to lack the sequence corresponding to exon 10 of the LHR gene in other mammalian species.

marmoset monkey LHR

LHR gene


Input: In unc-4 mutants, VA motor neurons assume the pattern of synaptic input normally reserved for their lineal sister cells, the VB motor neurons; the loss of normal input to the VAs produces a distinctive backward movement defect.

unc-4 mutants


Input: Intracavitary irradiation was carried out with a microSelectron HDR afterloading device and usually 22. 5 Gy was given in three fractions repeated weekly.


Input: No patient in group 2 developed HAT (P = 0. 006 versus group 1).


Input: Sequence comparison of the 0. 38 kb promoter sequence with the promoters of the Sm-E gene and U1 snRNA genes revealed several homologous motifs, suggesting that genes encoding the snRNP components may be coordinately regulated.

Sm-E

U1 snRNA genes


Input: METHODS: The responses of a 31-year-old woman with complex regional pain syndrome type I (reflex sympathetic dystrophy) to a thermal grill were evaluated before and after stellate ganglion block.


Input: More recently, however, a number of developments such as the successful use of the inhaled steroid budesonide and oral dexamethasone have reinforced the argument for using steroids.


Input: In AcMNPV-infected Sf9 cells, late transcription initiation is detected from only two upstream TAAG sites and not from three downstream TAAG sites.


Input: Mutation of nucleotides adjacent to the AP-1 cis-response elements had no effect on trans-activation.

AP-1 cis-response elements


Input: The close proximity of the two neuropeptide Y receptor genes suggests that they have evolved from a gene duplication event with the small intron interrupting the coding sequence of the y1 gene being converted into a functional sequence within the y5 gene, while the reverse complementary sequence was utilized as an alternatively spliced 5' exon for the y1 gene.

neuropeptide Y receptor genes

y1 gene

y5 gene

y1 gene


Input: The mammalian phosphoinositide 3-kinases (PI3Ks) p110alpha, beta, and delta form heterodimers with Src homology 2 (SH2) domain-containing adaptors such as p85alpha or p55 (PIK).

mammalian phosphoinositide 3-kinases

p110alpha, beta, and delta

Src homology 2

PIK


Input: In the studies reported here, the 5'-flanking region of the human gp130 gene was isolated and the transcription initiation sites were mapped.

human gp130 gene


Input: Localization of the cytokine response element by 5'-deletion analysis and site-directed mutagenesis revealed a cis-acting binding site for activated STAT complexes.

STAT


Input: Insulin regulation of mitogen-activated protein kinase kinase (MEK), mitogen-activated protein kinase and casein kinase in the cell nucleus: a possible role in the regulation of gene expression.

Insulin

mitogen-activated protein kinase kinase

MEK

mitogen-activated protein kinase

casein kinase


Input: Sequencing of the three pag-3 alleles showed that two apparent null alleles encode a nonsense mutation before the zinc fingers and a missense mutation in the fourth zinc finger that changes a coordinating histidine to a tyrosine.

pag-3 alleles


Input: Here, a case of Sjogren' s syndrome is presented that was initially diagnosed because of dental complaints, and long-term treatment of Sjogren' s patients is discussed.


Input: Cloning and characterization of a cDNA encoding a bacteriophage-type RNA polymerase from the higher plant Chenopodium album.

bacteriophage-type RNA polymerase


Input: Similarly, overexpression of increasing concentration of COUP-TFI, but not COUP-TFI delta35, can squelch the silencing activity of the unliganded TRbeta.

COUP-TFI

COUP-TFI delta35

TRbeta


Input: In ciliates, both mechanisms are readily observed.


Input: Based on the occurrence of several transcription signals in the Thermus pyr promoter region and strong amino acid sequence identities (about 60%) between Thermus PyrR and the PyrR attenuation proteins of two Bacillus sp., we propose a regulatory mechanism involving transcriptional attenuation to control pyr gene expression in Thermus.

Thermus pyr promoter region

Thermus PyrR

PyrR attenuation proteins

pyr gene


Input: Diver respiratory responses to a tunable closed-circuit breathing apparatus.


Input: Measurement of the time constant of VO2 and oxygen pulse during constant work rate exercise are useful for the objective evaluation of the training effect of patients with COPD.


Input: Characterization of CR1 repeat random PCR markers for mapping the chicken genome.


Input: A reversed-phase high-performance liquid chromatographic (HPLC) method for the determination of cocaine metabolites produced in vitro by serum and liver esterases is described.

serum and liver esterases


Input: Mutational studies provide evidence to this end and indicate that the side chains of subdomain 4. 2 make specific contacts with the nucleotides at-35.


Input: It is concluded that fludarabine is a highly useful agent in CLL.


Input: Three different techniques of EA analysis were used in the study: 1) fast Fourier transformation (FFT) of EA in a broad band, 2) developed by us alternative method of non-harmonic expansion of the EEG curves taking into account their shape, 3) factor analysis of the EA spectral densities.


Input: Concomitantly, p34CDC2 histone H1 kinase activity increases in the former, but not in the latter cell lines, hence suggesting a role for this protein in radiation-induced cell death.

p34CDC2 histone H1


Input: Thus, the B-S mutant does not mimic efficiently the chloroplastic GAPDHs, and long-range and/or second-layer effects, not easily predictable from visual inspection of three-dimensional structures, need to be taken into account for designing a true " chloroplastic-like " mutant of cytosolic GAPDH.

chloroplastic GAPDHs

cytosolic GAPDH


Input: Computer software assisted ordering (CSAO) was developed to integrate PN Ca: P solubility with clinical data to improve parenteral Ca and P administration.


Input: A similar chimera was assembled from the two halves of the molecule expressed separately in different bacteria and refolded together.


Input: The conditions for obtaining titanium dioxide from the substrates titanium tetrachloride and oxygen and applying this to a surgical stainless steel of the type 316L by the plasma assisted chemical vapour deposition method have been determined.


Input: All six genes were cloned and characterised.


Input: We analysed two regions involved in preS2/S gene transcription of the HBV adw subtype: the diverged TATA box and a putative initiator element.

S gene


Input: Indeed the predicted folding of the 5' and 3'-untranslated regions revealed patterns of stem and loop structures conserved for all tick-borne flaviviruses suggesting a purifying selection for preservation of essential RNA secondary structures which could be involved in translational control and replication.


Input: EGF acts primarily by means of transactivation domain AF-1, whereas cAMP acts via transactivation domain AF-2 of the ER.

EGF

ER


Input: To determine which region of SulA is essential for the inhibition of cell division, we constructed a series of N-terminal and C-terminal deletions of SulA and a series of alanine substitution mutants.

SulA

SulA


Input: We have inactivated Krox-20 by homologous recombination in ES cells and demonstrated that the mutation leads to the deletion of r3 and r5.


Input: Recently we have performed a detailed analysis of specific neuronal populations affected by the mutation which shed new light on the role of Krox-20 in the segmentation and on the physiological consequences of its inactivation.


Input: Consistent with its role in p53 ubiquitination, mE6-AP was found both in the nucleus and cytosol, while Nedd-4 was found only in the cytosol.


Input: SPP also induced transient tyrosine phosphorylation of focal adhesion kinase (p125 (FAK)), a cytosolic tyrosine kinase that localizes in focal adhesions, and of the cytoskeleton-associated protein paxillin.

SPP

focal adhesion kinase

FAK

cytosolic tyrosine kinase

cytoskeleton-associated protein paxillin


Input: Evaluation of myocardial perfusion by 99mTc-tetrofosmin SPECT before and after emergent percutaneous transluminal coronary angioplasty for acute myocardial infarction


Input: A neonatal alloimmune thrombocytopenia with an HPA-3a (Baka) incompatibility was confirmed.


Input: Most eukaryotic mRNAs are translated by a " scanning ribosome " mechanism.


Input: Comparison of Tc-99m sestamibi perfusion imaging and echocardiography using an arbutamine infusion for the detection of coronary artery disease.


Input: Five of these have ocular or oculocutaneous albinism.


Input: Comprising a 126-nucleotide 5' untranscribed genomic sequence and a 466-nucleotide 5' noncoding cDNA sequence, the 592-nucleotide 5' CpG island lacked TATA and CAAT boxes but displayed a high G + C content, was enriched for CpG dinucleotides, and contained a potential Sp1-binding site, i. e., features compatible with a housekeeping gene.

Sp1-binding site


Input: SSeCKS (pronounced essex) encodes a major protein kinase C substrate, the expression of which is down-regulated in src-and ras-transformed rodent fibroblasts but not in raf-transformed rodent fibroblasts (X.

SSeCKS

protein kinase C

src

ras


Input: The 1. 1-and 1. 3-kb mRNA species were found only in the heart, and the 2. 6-kb species was found in the heart, kidney and brain, but not in skeletal muscle or liver.


Input: Taurine deficiency significantly depressed the amplitude of OP1 and OP4.


Input: We have found that mcs4-cells are defective at activation of Spc1 in response to various forms of stress.


Input: The extended rat SP-A isoforms were enriched in the more fully glycosylated and multimeric SP-A species separated on SDS-PAGE gels.

rat SP-A isoforms

SP-A


Input: Alternative splicing of CDC25B may therefore contribute to the control of cell proliferation.


Input: This investigation was undertaken to determine whether consuming several small feedings of preexercise carbohydrate (CHO), rather than a single bolus, would affect blood glucose and insulin responses during rest and exercise.

insulin


Input: The findings suggest that ERP effects of distinct memory processes are differentially influenced by the encoding instructions.


Input: This coat protein consists of Sar1p, the Sec23p protein complex containing Sec23p and Sec24p, and the Sec13p protein complex containing Sec13p and a novel 150-kDa protein, p150.

coat protein

Sec23p protein complex

Sec13p protein complex

150-kDa protein, p150


Input: The second primary mutant contained a proline-to-leucine change at position 243 (P243L).


Input: Induction of correctly spliced germline transcripts is necessary to target a switch region for recombination and switching.


Input: Thus, the PCE binds a Pbx dimer partner that behaves unlike Class I Hox proteins.

PCE

Pbx dimer partner

Class I Hox proteins


Input: Furthermore, the NH2-terminal portion of the homologue was not translocated into the outer membrane without its COOH-terminal part.


Input: Molecular cloning and characterization of a cDNA, CHEMR1, encoding a chemokine receptor with a homology to the human C-C chemokine receptor, CCR-4.

human C-C chemokine receptor


Input: Organization of the human LU gene and molecular basis of the Lu (a)/Lu (b) blood group polymorphism.

human LU gene

Lu (a)

Lu (b)


Input: Whereas the mutant enzymes terminate normally at the late terminator in T7 DNA (T (phi)) and rrnB T2, they fail to terminate at one of the termination sites of rrnB T1, and also fail to recognize the PTH and CJ signals.

rrnB

rrnB

PTH


Input: Cytological data suggest that the transgenes associate with a nucleolus.


Input: Light regulatory unit 1 (LRU1) is necessary for and sufficient to mediate light-dependent activation of the chalcone synthase (CHS) minimal promoter in Petroselinum crispum.

Light regulatory unit 1

chalcone synthase (CHS) minimal promoter


Input: The active state is terminated by hydrolysis of bound GTP, producing inactive ARF-GDP.

ARF-GDP


Input: We suggest that apart from the World Summit for Children' s under-five mortality target for the year 2000, intraregional targets to reduce geographical inequalities in under-five mortality should be specified.


Input: The immunophilin, which can be of the FK506-or cyclosporin A-binding class, binds to hsp90 via its tetratricopeptide repeat (TPR) domain, and different receptor heterocomplexes exist depending upon which immunophilin occupies the TPR-binding region of hsp90.

immunophilin

immunophilin


Input: Sequencing of the facB gene revealed that it encodes a protein that contains an N-terminal GAL4-like Zn (II) 2Cys6 (or C6 zinc) binuclear cluster for DNA binding, leucine zipper-like heptad repeat motifs and central and C-terminal acidic alpha-helical regions, consistent with a function as a DNA-binding transcriptional activator.

facB gene


Input: Overexpression of Sed5p allowed growth in the absence of Vti1p.


Input: The coding region of mkh1 is contained within a single exon encoding a 1, 116-amino-acid protein.


Input: Strikingly, stoichiometric association of p107 or p130 with either cyclin E-cdk2 or cyclin A-cdk2 negated the activities of these kinases.

cyclin E

cyclin A


Input: Furthermore, p150 suppresses actin gelation, which is induced by smooth muscle alpha-actinin.

actin

smooth muscle alpha-actinin


Input: Denaturation of the simian virus 40 origin of replication mediated by human replication protein A.

human replication protein A


Input: This effect was abolished once the EM motif in the promoter-reporter construct was mutated, thus suggesting that the synergistic transactivation function of the TEF-1-Max heterotypic complex is mediated through binding of the complex to the EM motif.

Max


Input: Interdigitated residues within a small region of VP16 interact with Oct-1, HCF, and DNA.

HCF


Input: With a view to identifying other important U14 interactions, a stem-loop domain required for activity of Saccharomyces cerevisiae U14 RNAs (the Y domain) was first subjected to detailed mutational analysis.

Saccharomyces cerevisiae U14 RNAs


Input: Based on these results and because Cdc68 has been implicated as a regulator of chromatin structure, we postulate that polymerase alpha may interact with these proteins to gain access to its template or to origins of replication in vivo.

polymerase alpha


Input: Even when the inoculum size was reduced sixfold (i. e., 6 x 10 (6) CFU/mouse), 50% of the RB6-8C5-treated animals died within 6 days.


Input: In 12 cases (3. 1%), a change in stage resulted.


Input: Promoter recognition algorithms identified divergent promoter elements within the CpG island, which lies between the ATM and E14/NPAT genes, and provide evidence for a putative second ATM promoter located within intron 3, immediately upstream of the first coding exon.

ATM

NPAT

ATM promoter


Input: Recombinant soluble pMCP that lacked transmembrane and cytoplasmic domains had factor I cofactor activity in C3b cleavage, indicating that it is functionally, as well as structurally homologous to MCP.

Recombinant soluble pMCP

factor I cofactor

MCP


Input: Clinical and haematological signs are not specific in this setting, and the diagnosis relies on histological features, mainly bone marrow examination.


Input: Spatial zones for muscle coactivation and the control of postural stability.


Input: Liquid chromatographic separation was achieved on a Zorbax RX C8 analytical column using gradient elution.


Input: The linear plasmid pDHL1 from Debaryomyces hansenii encodes a protein highly homologous to the pGKL1-plasmid DNA polymerase.

pGKL1-plasmid DNA polymerase


Input: The p20-CGGBP gene is conserved among mammals but shows no homology to non-vertebrate species.

p20-CGGBP gene


Input: The data suggest that like in yeast, in plants a certain subfamily of UBC is specifically involved in the proteolytic degradation of abnormal proteins as result of stress.

UBC


Input: The pWP-19 also encodes the AAV inverted terminal repeats for integration and replication and the herpes virus thymidine kinase promoter-driven gene for neomycin resistance (neoR).

thymidine kinase

neoR


Input: We have developed a rAAV-mediated gene transfer system for the rat preproinsulin II gene.

rat preproinsulin II gene


Input: A striking feature of the recombinant H19 allele is the occurrence of a parental imprint set on the neo replacement cassette.

recombinant H19 allele

neo replacement cassette


Input: This indicates that recruitment of large T antigen to the rRNA promoter by SL1 constitutes a crucial step in the activation process.

large T antigen


Input: In 1990, an International Commission for the Certification of Eradication of Poliomyelitis Eradication (ICCPE) was established by the Pan American Health Organization to eventually determine if transmission was interrupted.


Input: Functional and regulatory analysis of the two copies of the fixNOQP operon of Rhizobium leguminosarum strain VF39.

fixNOQP operon


Input: This study describes a new MADS box gene, nmhC5, which along with nmh7 (J.

MADS box gene


Input: Lung and multi-system damage were early indicators of poor outcome in severe non-fatal disease.


Input: The results suggest that the bulbospinal micturition reflex evoked by bladder filling and L-dopa involves a descending pathway where transmission is partly mediated by spinal alpha 1-adrenoceptors.

spinal alpha 1-adrenoceptors


Input: Disturbance of regeneration in the above pathological condition results from the damage to tunica propria and loss of its function for the support of structural homeostasis.


Input: Sternocleidomastoid, neck or facial muscles wasting were also found in three cases.


Input: Identification of five new genes, closely related to the interleukin-1beta converting enzyme gene, that do not encode functional proteases.

interleukin-1beta converting enzyme gene


Input: The C-terminal mature region is highly conserved in other serine carboxypeptidases.

serine carboxypeptidases


Input: Here we demonstrate that AML1a, which solely has no effects as a transcriptional regulator, dominantly suppresses transcriptional activation by AML1b, and that AML1a exhibits the higher affinity for DNA-binding than AML1b.


Input: However, using ATL-16T nuclear extract, we demonstrated that GATA-4 is the only GATA-binding protein that forms specific DNA-protein complex with the-70 GATA site.

GATA-binding protein


Input: The impact of social support on the relationships of gay male couples is examined.


Input: The ubiquitously expressed hypoxia-inducible factor-1 (HIF-1) is involved in expression of a large number of oxygen-regulated genes.

hypoxia-inducible factor-1


Input: Stromelysin-1, matrix metalloproteinase-3 (MMP-3), is an important endopeptidase selectively expressed by somatic cells in organ tissues.

matrix metalloproteinase-3


Input: Tendon also shows a degree of extensibility.


Input: Surveillance for preeclampsia was conducted by personnel unaware of treatment-group assignments, using standardized measurements of blood pressure and urinary protein excretion at uniformly scheduled prenatal visits, protocols for monitoring these measurements during the hospitalization for delivery, and reviews of medical records of unscheduled outpatient visits and all hospitalizations.


Input: DNase I footprint analysis identified a protected region from-37 to-53.

DNase I


Input: We isolated several overlapping A-phage and cosmid clones that cover more than 100 kb of human DNA and contained the entire VDR gene.

VDR gene


Input: Here, we correlate Dox effects on cell cycle with changes of E2F/DP complexes and activity in differentiating C2C12 myocytes.

DP


Input: The products of the Saccharomyces cerevisiae CIN1, CIN2 and CIN4 genes participate in a nonessential pathway required for normal microtubule function.

Saccharomyces cerevisiae CIN1

CIN4 genes


Input: Vitrectomy was still a significant risk factor when macular holes were excluded.


Input: Invited editorial on " Acute and chronic effects of exercise on leptin levels in humans ".

leptin


Input: Both promoters lack a TATA box, and Pint belongs to the MED-1 class of promoters, which initiate transcription at multiple sites.

Pint


Input: Serum response factor (SRF), a member of an ancient family of DNA-binding proteins, is generally assumed to be a ubiquitous transcription factor involved in regulating growth factor-responsive genes.

Serum response factor

SRF


Input: We have initiated studies to identify candidate signal transducers that associate with the cytosolic domain (cd) of the IL-1R.


Input: In this study, we sought to identify mechanisms that could account for the antimitogenic effects of IL-4.


Input: Pseudomonas aeruginosa strains infecting patients with cystic fibrosis (CF) acquire a mucoid phenotype due to overproduction of alginate.


Input: Activity of palgD in the cysB mutant, in CHA and in the non-mucoid strain PAO was assessed by the use of a transcriptional algD-xylE fusion.

palgD

cysB mutant

algD

xylE


Input: The Man9-mannosidase specificity of the cDNA construct was verified by the observation that all peptide sequences derived from a previously purified, catalytically active 49-kDa fragment were found within the coding region.


Input: A soluble 62-kDa protein was produced without the proteolytic processing by inserting the coding sequence of amino acids 112 to 660 of ORF-2 in a baculovirus expression vector and using the corresponding virus to infect Sf9 cells.


Input: The CPK-MB isoenzyme showed no percentage increase of total CPK higher than 5%, measured at 6, 12, and 24 h after the shock, independent of the number of attempts of cardioversion.

CPK-MB

CPK


Input: Serum and peritoneal dialysis fluid (PDF) were collected for assay throughout the course of the study and for 5 days thereafter.


Input: Results support the hypothesis that endogenous corticotropin-releasing factor, perhaps acting at a peripheral binding site, suppresses the active behavioral response characteristic of pups during the early phase of isolation in novel surroundings.

endogenous corticotropin-releasing factor


Input: Liver regional blood volume (LRBV) is altered by several disease states and various drugs.


Input: The gray matter NAA/mI ratio clearly separated the two groups.


Input: As with the murine and human mb-1 genes, the 5' region of the bovine mb-1 gene lacked a TATA box.

murine and human mb-1 genes

bovine mb-1 gene


Input: Chloramphenicol acetyltransferase assays examining the ability of IE86 to repress activity from the HCMV major IE promoter or activate the HCMV early promoter for the 2. 2-kb class of RNAs demonstrated the functional integrity of the IE86 protein.

Chloramphenicol acetyltransferase

IE promoter

HCMV early promoter

IE86 protein


Input: ORF M1 has striking homology to poxvirus serpins, while ORF M11 encodes a potential homolog of Bcl-2-like molecules encoded by other gammaherpesviruses (gene 16 of HVS and KSHV and the BHRF1 gene of EBV).

serpins

Bcl-2-like molecules

gene 16

BHRF1 gene


Input: Transcription factor binding sites downstream of the human immunodeficiency virus type 1 transcription start site are important for virus infectivity.


Input: Crystal structure of an oligomer of proteolytic zymogens: detailed conformational analysis of the bovine ternary complex and implications for their activation.


Input: Through transient transfection of NIH3T3 fibroblast cells and gel mobility shift assays, the functional binding site was localized to a short region (-318 to-303 bp from the transcription start site) which has a CTCCC sequence.


Input: We suppose that this injection speed (0. 1-0. 2 ml. s-1) is suitable for spinal anesthesia.


Input: The current study demonstrates that T3-activated transcription of the NADPH: cytochrome P450 oxidoreductase (P450R) gene is dependent on the thyroid hormonal status of the animal, with both transcriptional and post-transcriptional pathways being important in regulating the cellular P450R mRNA level.

NADPH: cytochrome P450 oxidoreductase


Input: The behavior of different types of polytetrafluoroethylene (PTFE) prostheses in the reparative scarring process of abdominal wall defects.


Input: The control group revealed significantly higher levels of coagulation factors II, V and VIII, compared to the index group.

coagulation factors II, V and VIII


Input: Stereotactic radiofrequency lesioning of the hamartoma resulted in seizure remission without complications 20 months after surgery.


Input: If E. coli is present in any source water sample, the borehole and any directly connected borehole should be embargoed.


Input: The following technique describes a modification to a bar superstructure that provided the advantages of convenience, security, and consistent positioning even though one implant was lost and the angulation of implants limited accuracy.


Input: In summary, DDC express receptors for a broad panel of cytokines, even receptors for cytokines whose effects on DC are still unknown (i. e.


Input: Its potential as a diagnostic tool for epidemiological surveillance was assessed in comparison with three other diagnostic tests: stool examination, ELISA with soluble egg antigen (SEA) and the circumoval precipitin test (COPT).

soluble egg antigen

SEA

precipitin


Input: These analyses demonstrate that the dUTPase isoforms are encoded by the same gene with isoform-specific transcripts arising through the use of alternative 5' exons.

dUTPase


Input: The selective 5-HT3 receptor antagonists ramosetron (YM060), YM114 (KAE-393), granisetron and ondansetron, and the substituted benzamides (5-HT4 receptor agonist/5-HT3 receptor antagonists) cisapride mosapride and SC-53116 dose-dependently enhanced gastric emptying in normal rats.

5-HT3 receptor

5-HT4 receptor

5-HT3 receptor


Input: Re-evaluation of the biopsy specimen from the right hip taken at the time of the initial operation showed areas of chondrosarcoma arising in the background of synovial chondromatosis.


Input: All corneal buttons were processed for histopathologic and electron microscopic studies.


Input: 3600-fold to apparent homogeneity with a 41% yield by affinity chromatography utilizing DNA-cellulose; the purity of the final preparation was assessed by SDS/PAGE, lack of contamination by other nucleases and production of a monospecific antibody against the enzyme.


Input: Human thymine-DNA glycosylase maps at chromosome 12q22-q24. 1: a region of high loss of heterozygosity in gastric cancer.

Human thymine-DNA glycosylase


Input: FOG is coexpressed with GATA-1 during embryonic development and in erythroid and megakaryocytic cells.

FOG


Input: We further found that KmMig1p is fully functional when expressed in S. cerevisiae.


Input: Autoimmune neutropenia (AIN) is a frequent cause of chronic neutropenia especially in youngest children.


Input: With regard to the characteristic expression pattern of DJ protein and its conspicuous repeat units possible functional roles are discussed.

DJ protein


Input: Future analysis of long-term outcome measures of performance with the implant will confirm or dispute the benefit of ear selection using the Prom-EABR.


Input: This protein, designated p532 based on its predicted molecular weight (EMBO J 15: 5738, 1996), contains multiple structural domains including two regions of seven internal repeats highly related to the cell cycle regulator RCC1, a guanine nucleotide exchange factor for the small GTP-binding protein Ran, seven beta-repeat domains characteristic of the beta subunit of heterotrimeric G proteins, three putative SH3 binding sites, a putative leucine-zipper and a carboxy-terminal HECT domain characteristic of E3 ubiquitin-protein ligases.

guanine nucleotide exchange factor

small GTP-binding protein Ran

heterotrimeric G proteins

SH3 binding sites

carboxy-terminal HECT domain

E3 ubiquitin-protein ligases


Input: Thus, the L45 sequence between kinase subdomains IV and V specifies TGF-beta responsiveness of the type I receptor.

TGF-beta

type I receptor


Input: The two mouse lines carrying the unfragmented Hnf3g-lacZ YAC showed tissue-specific, copy number-dependent and position-independent expression, proving that 170 kb of the Hnf3g locus contain all elements important in the regulation of Hnf3g.

lacZ


Input: Second, plasmid-derived transgenes and gene targeting of the endogenous Hnf3g gene locus were used to demonstrate that the 3'-flanking region of the gene is necessary and sufficient to direct reporter gene expression in liver, pancreas, stomach and small intestine.

endogenous Hnf3g gene locus


Input: Here we have tested the hypothesis that the short transcripts, or proteins translated from the short transcripts, are also required for respiration.


Input: Upstream activating sequences (UASs) derived from known Ste12p-dependent genes have previously been characterized to require either multiple PREs or a single PRE coupled to a binding site for a second protein.


Input: Prominent protein kinase cascades are those that activate mitogen-activated protein (MAP) kinases.

mitogen-activated protein (MAP) kinases


Input: JNK is additionally regulated by the Ras-related G proteins Rac and Cdc42.

JNK

Ras

G proteins Rac


Input: In this study, we tested this notion by using ligation-mediated PCR to assess the formation of recombination-activating gene (RAG)-dependent double-strand breaks (DSBs) at RSSs 3' of Ddelta3 and 5' of Jdelta1.

recombination-activating gene

RAG


Input: Recent studies have demonstrated that Cbl, the 120-kDa protein product of the c-cbl proto-oncogene, serves as a substrate of a number of receptor-coupled tyrosine kinases and forms complexes with SH3 and SH2 domain-containing proteins, pointing to its role in signal transduction.

Cbl

c-cbl proto-oncogene

receptor-coupled tyrosine kinases


Input: Although neither constitutively activated MEK (MEK-2E) nor v-Src was sufficient individually to differentiate the H19-7 cells, coexpression of constitutively activated MEK and v-Src induced neurite outgrowth.

MEK

v-Src

MEK

v-Src


Input: This mechanism is in contrast to other cases of splicing regulation by PTB, in which the protein represses the splice site to which it binds.

PTB


Input: While important for ligand-dependent transactivation, this interaction surface is not directly involved in transrepression of AP-1 activity.


Input: Gal4-Eed fusion protein represses transcription of a reporter gene driven by a promoter that contains Gal4-binding DNA elements.

Eed

Gal4-binding DNA elements


Input: In situ hybridization analysis in adult rat tissue reveals good correlation with this pattern and indicates that drm mRNA is most highly expressed in nondividing and terminally differentiated cells, such as neurons, type 1 lung cells, and goblet cells.

drm mRNA


Input: A 2 years and 9 months old female patient, with the diagnosis of Weaver syndrome is reported.


Input: Confirming measures made by nurses and additionally, ABPM for women, seem to lessen this effect.


Input: In a series of patients with neuroinfection, Lyme disease, Guillain Barre syndrome, demyelinization, partial or generalized, epilepsy, we have investigated antiphospholipid antibodies of IgG and IgM subtypes, together with anticoagulant factors, member of thrombocytes, sedimentation rate of erythrocytes.

IgG

IgM


Input: Second, the membrane expression of alpha and beta subunits was mimicked by cholesterol and 17-ketocholesterol, both of which inhibit HMG-CoA reductase.

HMG-CoA reductase


Input: Our results suggest that Lrp binds a central palindromic site, interacting predominantly with the major groove of its DNA target, and that additional dimers bind to flanking sites to form a nucleoprotein activation complex.

Lrp


Input: Sustained activation of p21 expression is proposed to be a distinguishing feature of the activity of NGF that contributes to PC12 growth arrest during differentiation

NGF


Input: The remaining 80 patients underwent recordings of ECG, beat-to-beat arterial oxygen saturation (SaO2), and respiration during both spontaneous and controlled breathing.


Input: The mean total white cell count increased from a baseline of 11. 3 x 10 (9)/L (SD 2. 3) to 16. 2 x 10 (9)/L (SD 4. 6) on day 1, normalising thereafter.


Input: In all experimental eyes, there was a marked reduction, but never a complete absence of adrenergic nerves in the iris.


Input: These lesions may be treated by propranolol or phentolamine.


Input: Furthermore, ICER negatively autoregulates the alternative promoter, thus generating a feedback loop.

ICER


Input: This night-day oscillation is driven by the endogenous clock (located in the suprachiasmatic nucleus, SCN).


Input: The Ogg1 protein efficiently cleaves a DNA duplex where a preformed AP site is placed opposite a cytosine (AP/C).

Ogg1 protein

AP site


Input: A consensus sequence indicates a highly conserved lysine residue, K120 of endonuclease III or K241 of Ogg1, respectively.

endonuclease III


Input: Overall, these findings demonstrate that mutations E768D and V804L are gain-of-function mutations that confer to the long RET isoform the capacity to exert a biological effect, although these mutations are more weakly activating than the MEN2A and MEN2B mutations.

long RET isoform


Input: Volunteers were irradiated on their backs with suberythemal UV daily for 5 d after application of the sunscreens and their base lotion to different sites.


Input: E2F is a heterodimeric complex consisting of E2F family members (1-5) and DP proteins (1-3).

E2F family members (1-5)

DP proteins (1-3)


Input: EBER1 mRNA, a consistent marker of viral latency, was positive in all PEL cases, although at lower levels than in the non-PEL controls due to EBER1 expression by only a variable subset of lymphoma cells.

EBER1 mRNA


Input: METHODS: Four hundred fifty patients with the diagnosis of squamous cell carcinoma of the oral cavity received their primary treatment at Roswell Park Cancer Center (RPCI) from 1971 to 1991.


Input: Therefore, use of presaturation is recommended for myocardial motion studies using cine PC velocity data.


Input: In addition, the DNA-binding experiments show that either one of the two MADS domains of a dimer can be sufficient to confer a particular DNA-binding specificity to the complex and that sequences outside the amino-terminal basic region of the MADS domain can, in some cases, contribute to the DNA-binding specificity of the proteins.

MADS domains

MADS


Input: PURPOSE/OBJECTIVES: To examine the question of whether an early first full-term pregnancy (FFTP) protects against breast cancer and whether interruption of the pregnancy with an induced abortion increases breast cancer risk.


Input: Two related studies conducted over a five week period measured and typed HLA-DQA1 from accumulated DNA on autopsy room and Forensic DNA Laboratory structures.


Input: Thirty-two rats were divided into four groups.


Input: Mutagenesis of the pecT regulatory region revealed the presence of two sites in which insertions reproduced the pec-1 phenotype.

pecT


Input: BRCA1, a familial breast and ovarian cancer susceptibility gene encodes nuclear phosphoproteins that function as tumor suppressors in human breast cancer cells.


Input: The alternative splice sites for the smallest adducin isoform, beta-3, are alternative donor and acceptor sites within exons 7 and 12.

adducin


Input: Two new temperature-sensitive alleles of SEC3, 1 of 10 late-acting SEC genes required for targeting or fusion of post-Golgi secretory vesicles to the plasma membrane in Saccharomyces cerevisiae, were isolated in a screen for temperature-sensitive secretory mutants that are synthetically lethal with sec4-8.

SEC genes


Input: Cell 49, 753-761].


Input: Twenty-one ELISA-positive sera were tested and confirmed positive by plaque reduction neutralization testing.


Input: Patients received either diltiazem CD 180 mg or placebo once/day in combination with existing antianginal therapy.


Input: Reactive lymphoid hyperplasia was found in 15 cases, 2 cases had angiofollicular lymphoid hyperplasia or Castleman' s disease, atypical lymphoid hyperplasia suggestive of malignant lymphoma was observed in 3 cases, and malignant lymphoma was diagnosed in the remaining 4 cases.


Input: Tyrosine 1356 forms a multisubstrate binding site for the Grb2 and Shc adaptor proteins, the p85 subunit of phosphatidylinositol 3'-kinase, phospholipase Cgamma, and a phosphatase, SHP2.

Shc adaptor proteins

p85 subunit

phosphatidylinositol 3'-kinase

phospholipase Cgamma


Input: Then we correlated HRCT findings with the clinical features, pulmonary functions and methacholine PC20 (PC20M) and studied their clinical significance.


Input: Gtx mRNA accumulates in parallel with the RNAs encoding the major structural proteins of myelin, myelin basic protein (MBP), and proteolipid protein (PLP) during postnatal brain development; Gtx mRNA decreases in parallel with MBP and PLP mRNAs in the brains of myelin-deficient rats, which have a point mutation in the PLP gene.

Gtx mRNA

myelin basic protein

MBP

proteolipid protein

PLP

Gtx mRNA

MBP

PLP mRNAs

PLP gene


Input: Contacts between Bacillus subtilis catabolite regulatory protein CcpA and amyO target site.

CcpA

amyO


Input: They also interact with GTPase activating proteins encoded by IRA1 and IRA2.

GTPase activating proteins


Input: For E2, each cat was given either 5 (group [G] 1) or 10 (G2) mg of itraconazole/kg (capsules) twice daily for 6 weeks.


Input: RESULTS: For E1, itraconazole plasma drug concentration extrapolated to time zero (IV dose) was 5. 2 +/-1. 4 micrograms/ml, and mean residence time (MRT) was 37 +/-16 hours.


Input: The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p.


Input: G-CSF was started on day 3 of each cycle (5 microg/kg/day) and was stopped the day before the last apheresis or when absolute neutrophil count was above 0. 5 x 10 (9)/l.

G-CSF


Input: Supershift assays, using Jun and Fos family member-specific antibodies, showed that protein complexes formed by AtT-20 cell nuclear extracts bound to the c-jun AP-1 site were comprised of Jun family members, JunD, JunB, and cJun.

Jun and Fos family member-specific antibodies

c-jun AP-1 site

Jun

JunD

JunB

cJun


Input: The majority of PI kinase activity appeared to be cbl-associated after PRL stimulation.

PI kinase

cbl

PRL


Input: Mutation of Enh4, an essential GT-IIC-like enhanson in the context of the intact enhancer, abolishes silencer activity, and multimerized GT-IIC enhansons mimic the intact CSEn enhancer/silencer activities in BeWo and GC cells, respectively.

GT-IIC-like enhanson

GT-IIC enhansons

CSEn enhancer/silencer


Input: Using lectin-affinity chromatography, discordance between the pattern of O-glycosylation of SSBP and DSBP was demonstrated.

lectin

SSBP

DSBP


Input: Deletion mapping experiments determined that the sequences required for full activity in MSC-1 cells were included within 619 bp of the start site and identified several regions that demonstrated increased luciferase activity: the-583 bp to-564 bp,-503 bp to-484 bp, and-114 bp to-65 regions.

luciferase


Input: Furthermore, the PH and PTB domains are highly homologous (at least 40% identical) to those found in insulin receptor substrates 1, 2, and 3 (IRS-1, IRS-2, and IRS-3).

PH

insulin receptor substrates 1, 2, and 3


Input: The mouse extracellular signal-regulated kinase 2 gene.

extracellular signal-regulated kinase 2 gene


Input: As demonstrated by gel mobility shift analysis and supershift experiments, FIRE1, located between-516 and-498, is responsible for binding NF-Y.

NF-Y


Input: MR imaging of traumatic head injuries using FLAIR technique


Input: Gag protein sequence motifs of the NC domain of primate foamy viruses assumed to be involved in genome encapsidation are not conserved in FeFV.

Gag

NC domain


Input: Induction of B cell apoptosis by co-cross-linking CD23 and sIg involves aberrant regulation of c-myc and is inhibited by bcl-2.

sIg

c-myc


Input: Here, we report theoretical models for the Gla and EGF-1 modules of human PS constructed using prothrombin and factor X experimental structures.

EGF-1 modules

human PS

prothrombin

factor X


Input: The Cut9 subunit is likely to be a target for regulating APC/cyclosome function through protein-protein interactions and phosphorylation.

Cut9 subunit

APC/cyclosome


Input: The dose was 50 Gy/20 fractions/5 weeks.


Input: All the women received 13. 5 mg plain bupivacaine via subarachnoid injection at the L2-3 interspace.


Input: Far from this being the case, however, the measurement of apo B has met every reasonable standard of laboratory precision and reliability to allow its widespread introduction in clinical laboratories.

apo B


Input: Two soybean cDNA clones, SPK-3 and SPK-4, encoding putative protein kinases were isolated and characterized.


Input: Genes belonging to the ras superfamily encode low-molecular-weight GTP/GDP-binding proteins that are highly conserved in wide variety of organisms.

ras superfamily

low-molecular-weight GTP/GDP-binding proteins


Input: Ent-kaurene synthase from the fungus Phaeosphaeria sp.

Ent-kaurene synthase from the fungus Phaeosphaeria


Input: In this report, we identify and characterize two overlapping ELL functional domains that govern its interaction with RNA polymerase II and the ternary elongation complex.

ELL functional domains

RNA polymerase II


Input: Eight induced cDNA sequences were identified and designated message up-regulated during death (mud)-1-8.

message up-regulated during death (mud)-1-8


Input: The position, transcription orientation, and imprinted status of the genes immediately flanking Igf2r have been assessed.


Input: Here, we describe directional chromosome walking studies starting from D8S260 as well as D8S285.


Input: We describe a novel zinc finger protein, dsRBP-ZFa, isolated by screening an expression library with dsRNA.

zinc finger protein

dsRBP-ZFa


Input: Our results indicate that the retroplasmid reverse transcriptase is uniquely adapted to initiate cDNA synthesis by recognizing a 3' CCA sequence.

reverse transcriptase


Input: Nasal absorption was rapid, nasal bioavailability was 43%, and the iv and nasal elimination profiles were similar.


Input: Together, these results suggest that EGF/Ras/Raf induces transcription via combined activation of ATF3/c-Jun and a 52-kDa nuclear factor, whereas JunD acts as a repressor of this response.

EGF

Ras

Raf

c-Jun

JunD


Input: Together, these data support a model in which Tax anchors CBP to the HTLV-1 promoter, with strong transcriptional activation resulting from the CBP-associated activities of nucleosome remodeling and recruitment of the general transcription machinery.

Tax

CBP

HTLV-1 promoter

CBP


Input: DNase I and 1, 10-phenanthroline-copper footprinting of MURA-Mu1 TIR complexes indicate that MURA binds to a conserved approximately 32-bp region in the TIR of Mu1.

DNase I

MURA

MURA


Input: Various assays demonstrate promoter activity in this sequence that reproduces the normal control of E2F2 expression during a growth stimulation.


Input: In the adult mouse, ERR alpha is most highly expressed in kidney, heart, and brown adipocytes, tissues which preferentially metabolize fatty acids.

ERR alpha


Input: The MCAD nuclear receptor response element 1 (NRRE-1) interacts in vitro with ERR alpha expressed in COS-7 cells.

MCAD nuclear receptor response element 1

ERR alpha


Input: Mapping features of HIV-1 integrase near selected sites on viral and target DNA molecules in an active enzyme-DNA complex by photo-cross-linking.

HIV-1 integrase


Input: The complete response (CR) rate was 34% in the CODE with rhG-CSF group and 23% in the CODE alone group; the median survival was 59 and 32 weeks, respectively, in these groups (P = 0. 004).

rhG-CSF


Input: Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA.

Heterogeneous nuclear ribonucleoprotein A1


Input: In the first part of our study, the highest mutagenicity was revealed by TA98 strain without enzymatic activation, suggesting a direct-acting mutagenicity prevalence in diesel particulate.


Input: The addition of culture to the CC and CC: SW by-products resulted in pH values lower (P < 0. 05) than those without culture on Day 21, and the 15% carbohydrate treatment significantly reduced pH beyond the 5% carbohydrate.


Input: Myocardial interstitial edema is more likely a potential mechanism of diastolic dysfunction after DC shocks.


Input: Two experiments (N = 20 each) were carried out to explore the nature of ERP negativities in a visuospatial memory task and in an auditory spatial memory task, respectively.


Input: Biol.


Input: The pattern and timing of CARP mRNA expression, including transient expression in the tongue at 14. 5 days p. c., coincides with that of Nkx2. 5/Csx (a putative homolog of tinman, the Drosophila melanogaster gene responsible for cardiac development).

CARP mRNA

Nkx2. 5

Csx

tinman


Input: Overexpression of CARP in cardiomyocytes suppresses cardiac troponin C and atrial natriuretic factor transcription.

CARP

cardiac troponin C

atrial natriuretic factor


Input: Substrate specificity of the RNase activity of yeast RNA polymerase III.

RNase

yeast RNA polymerase III


Input: Electrophoretic mobility shift assays and competition experiments showed that site A is recognized by an NF1 protein.

NF1 protein


Input: Examination of the MMP-2 RE1 sequence revealed an incomplete Y-box sequence (CTGCTGGGCAAG), which specifically interacted with recombinant YB-1 on DMS protection footprinting analysis.

MMP-2 RE1 sequence

recombinant YB-1


Input: Hence, scs32 only partially suppressed the ts phenotype and was unable to suppress the Ino-phenotype of rpo26-31.


Input: In contrast, both Sp1 and ETS proteins are required to bring about full promoter activity in the Surf-1 direction.

ETS proteins


Input: We have subcloned into pBR322 deoxyribonucleic acid (DNA) sequences mapping either in the coding region, the 5' spacer, or the 3' spacer of the H2B histone gene.

H2B histone gene


Input: The combination of hydralazine hydrochloride and isosorbide dinitrate also improves survival, but direct comparison of both regimens provided evidence for a less favourable effect than that of the ACE inhibitors.

ACE


Input: To address mechanisms that define interactions of Site II regulatory factors with this cell cycle control element, we have investigated these determinants of transcriptional regulation at the G1/S phase transition in FDC-P1 hematopoietic progenitor cells.

Site II regulatory factors


Input: The goal of this study was to identify neuronal cell cultures that express RC3/neurogranin, to check whether they are sensitive to T3, and to examine the mechanism of regulation.

neurogranin


Input: Their circadian responses to both photic and non-photic cues were then tested.


Input: Human Duo contains a guanine nucleotide exchange factor (GEF) domain that is likely to be rac1-specific, a pleckstrin homology (PH) domain and spectrin-like repeat units.

Human Duo

guanine nucleotide exchange factor

GEF

pleckstrin homology

PH

spectrin-like repeat units


Input: ArgR was shown to be a dimer of two equal subunits, each with a molecular mass of 37, 000 Da.

ArgR


Input: Plasma leptin concentrations were higher in women than men, even after the adjustment for differences in fat mass (28 +/-3 ng/ml for women vs.

Plasma leptin


Input: Consistent with effects on STAT activation, altered SHP-1 expression also affected EGF-induced activation of the mitogen-activated protein kinase pathway; expression of SHP-1-(Cys--> Ser) inhibited activity of MEK by approximately 25%, whereas expression of SHP-1 resulted in a approximately 25% increase.

STAT

EGF

mitogen-activated protein kinase

SHP-1-(Cys--> Ser)

MEK


Input: This interaction involved the SH3 region of p50 (csk) and a proline-rich region (PPPLPERTPESFVLADM) outside the catalytic region of PTP-PEST.

SH3 region

csk

PTP


Input: Following 2. 5 Gy, HbO2 changes were minimal.


Input: Among 38 patients with inducible reentrant VTs who underwent electrophysiologic study (EPS), 10 VTs of six patients were selected.


Input: The cycle length of induced VT (n = 10 VTs) was 380 +/-41 msec.


Input: Transient hyperammonaemia in an adult German shepherd dog.


Input: We established that the 2. 6 kb mRNA V-1 and the 2. 3 kb GGT mRNA V-2 derive, by alternate splicing, from a primary transcript initiated on a distal promoter on the rat GGT gene.

GGT

rat GGT gene


Input: A specific distal promoter controls gamma-glutamyl transpeptidase gene expression in undifferentiated rat transformed liver cells.

gamma-glutamyl transpeptidase gene


Input: These repressor sites are pyrimidine rich and bind avidly to the polypyrimidine tract binding protein (PTB) in HeLa nuclear extracts as determined by UV crosslinking/competition assays.

polypyrimidine tract binding protein

PTB


Input: For higher expression of lipA in S. lividans, the gene was cloned next to the strong aphII promoter.

lipA

aphII promoter


Input: Within a 135-bp core homology region, the human HS12 enhancers are approximately 90% identical to the murine homolog and include several motifs previously demonstrated to be important for function of the murine enhancer; additional segments of high sequence conservation suggest the possibility of previously unrecognized functional motifs.

human HS12 enhancers


Input: The two cysteine residues located in this additional region may be involved in the formation of a disulfide bridge associated with the activation process of the catalytic activity.


Input: The por gene has been expressed, for the first time, in anaerobically grown Escherichia coli behind the isopropyl-beta-D-thiogalactopyranoside-inducible tac promoter, resulting in the production of POR in its active form.

por gene

tac promoter

POR


Input: When the downstream operator was altered, there was a fourfold reduction in reporter enzyme levels.


Input: The ORF3 mutant produced reduced levels of tabtoxin, indicating that ORF3 may have a role in T beta L biosynthesis.

tabtoxin

T beta L


Input: Refined Cox models using a CD4 count of 150/microL rather than 200/microL to distinguish I0 and I1 yielded a simplified model with better fit to the observed data.


Input: However, the published reports consist of a small number of patients and limited data.


Input: These transcripts contain the 5' ends of mature UbCRBP mRNAs; extend through UbCRBP, across the intergenic region, and a significant distance 3' into the enolase gene.

UbCRBP mRNAs

UbCRBP

enolase gene


Input: The distribution of cryptosporidia in the intestine and number of cryptosporidia per ileal villus on different DPI were also estimated for detailed characterization of the infection in kids as a model for experimental cryptosporidiosis.


Input: As a result, the subendocardial/subepicardial blood flow ratio (ENDO/EPI) increased from 0. 44 +/-0. 09 during control stenosis to 0. 85 +/-0. 13 after ITF 1129 (10 micrograms/kg/min i. v.) and to 0. 81 +/-0. 12 after NTG.


Input: Proteasomes are the multisubunit protease involved in the generation of peptides presented by MHC class I molecules.

MHC class I molecules


Input: Takahashi, H.