Input: However, artificially ventilated rats, pretreated with MK-801, were more sensitive (lethal cocaine dose, 76. 6 +/-8. 0 mg/kg, n = 5) than vehicle pretreated rats (129. 4 +/-15. 8 mg/kg, n = 6), indicating that MK-801 may increase both the respiratory and the cardiac toxicity of cocaine in urethane anesthetized rats.


Input: ENV also was secreted from P. pastoris using the S. cerevisiae alpha-factor prepro secretion leader and the S. cerevisiae invertase signal sequence.

ENV

S. cerevisiae alpha-factor prepro secretion leader

S. cerevisiae invertase signal sequence


Input: The following evidence indicates that the 69-kD protein is a common, rather than a U1-specific, protein, possibly associating with the snRNP core particles by protein-protein interaction.


Input: Praziquantel and Albendazole were found effective in the treatment of neurocysticercosis, but because of serious side effects encountered in some cases, the drugs should be used cautiously in selected cases only.


Input: Transcriptional analysis of a series of deletion mutants of the gene in the nuclear extracts prepared from the middle silk gland of 2-day-old fifth instar larvae revealed the presence of multiple cis-regulatory elements located both upstream and downstream of the initiation site.


Input: One of these elements, the homeodomain-binding element, was identified to mediate negative regulation.

homeodomain-binding element


Input: Monospecific antibodies raised against rat cytochrome P-450 1A1 recognized a protein in the hepatic microsomes of the double-crested cormorant, and also in those of the great blue heron (Ardea herodias), using immunoblotting.

rat cytochrome P-450 1A1


Input: Mutations in the nuclear gene CBP1 of Saccharomyces cerevisiae result in degradation of mitochondrially encoded cytochrome b (cob) RNA; thus, the cells are unable to respire.

nuclear gene CBP1 of Saccharomyces cerevisiae

mitochondrially encoded cytochrome b

cob


Input: The 5' ends of F3R late transcripts were located to an A within the sequence 5'-TAAAG, 41 nt downstream from the early promoter and 17 nt upstream from the initiation codon.


Input: NF-kappa B p65, p50, and Rel functionally synergize with C/EBP alpha, C/EBP beta, and C/EBP delta.

NF-kappa B p65

Rel

C/EBP alpha

C/EBP beta

C/EBP delta


Input: It could be detected exclusively in the culture medium of cDNA-transfected COS cells.


Input: The first group of sequential BMB showed a significant progress to myelofibrosis in so-called " Chronic Megakaryocytic-Granulocytic Myelosis "--CMGM-, which corresponds to Agnogenic Myeloid Metaplasia-AMM-in 72. 4% (21/29 patients), as well as in CML with megakaryocytic increase-CML. MI-in 39. 2% (20/51).


Input: These lesions were asymptomatic, but both were characterized clinically by central ulceration.


Input: Release of this selective pressure, however, gave way to homologous resolution of the cointegrate structures.


Input: The equivalent of the third ligand, H-87, is T-47 in the PSTAIRE sequence motif.


Input: Botulinum toxin: preferred treatment for hemifacial spasm.

Botulinum toxin


Input: 2-AP induced marked, steady rises in mRNA accumulation from both transfected and chromosomally integrated HIV-1 constructs but no increases from an endogenous gene encoding gamma-actin or glucose 6-phosphate dehydrogenase.

gamma-actin

glucose 6-phosphate dehydrogenase


Input: On the other hand, the structures of the b-Zip domain are well conserved among these Maf-related proteins.

Maf-related proteins


Input: The Functional Independence Measure: a comparative study of clinician and self ratings.


Input: Polymyxin B was given intravenously for 1 week postburn in doses designed to neutralize circulating endotoxemia.


Input: SvO2 can be determined in vitro and in vivo.


Input: The transverse relaxation time (T2) and apparent diffusion coefficient of water were determined.


Input: These findings suggest a functional cross-talk between RB protein and p21ras, which balances the cell phenotype between normal and transformed states.

RB protein


Input: The other transmembrane regions as well as the nucleoplasmic domain are not required for sorting.


Input: These cell lines, selected for the ability to support the replication of a temperature-sensitive VP5 mutant, were used to isolate VP5 and VP23 null mutants.

VP5 mutant


Input: The E1 nuclear transport motif is highly conserved in the animal and human papillomaviruses and is encoded in a similar region in the related E1 genes.

E1 nuclear transport motif

E1 genes


Input: The E1 replication protein of bovine papillomavirus type 1 contains an extended nuclear localization signal that includes a p34cdc2 phosphorylation site.

E1 replication protein


Input: In the present study, beta-funaltrexamine (beta-FNA) and naltrindole (NTI) (nonequilibrium mu-and delta-antagonist, respectively) were used to precipitate withdrawal in butorphanol-dependent rats.


Input: Mutations at the extreme C-terminus of EIAV Tat impaired both RNA binding and activation domain functions, suggesting effects on secondary or tertiary structure.

EIAV Tat


Input: This observation suggests that the methyl-directed repair system utilizes the proximal d (GATC) sequence to direct correction.


Input: Lithium phthalocyanine (LiPc) is a prototype of another generation of synthetic, metallic-organic, paramagnetic crystallites that appear very useful for in vitro and in vivo electron paramagnetic resonance oximetry.


Input: IE2-IE2 interactions were mapped to a domain containing a putative helix-turn-helix motif located near the C terminus of IE2, between amino acids 456 and 539.


Input: Precipitation using GST fusion proteins containing Fyn SH2, SH3, and SH2/SH3 domains revealed that PI 3-kinase bound principally to the SH3 domain of Fyn.

GST fusion proteins

Fyn SH2

PI 3-kinase

SH3 domain

Fyn


Input: A sheep testicular cDNA library constructed in pcDNA1 vector was screened with a probe generated by polymerase chain reaction (PCR) and corresponding to a 1. 6 kb fragment of the rat luteinizing hormone receptor cDNA.

rat luteinizing hormone receptor cDNA


Input: Two putative Rev proteins with apparent molecular masses of 18 and 16 kDa were expressed by p2/2 and p176, while p20 expressed only a 16-kDa species.

Rev proteins


Input: Defects of fibrillin (FBN1), a glycoprotein component of the extracellular microfibril, cause Marfan syndrome.

fibrillin


Input: A region approximately 100 bp upstream from the transcription start point of virB was identified as being necessary for full activation of this promoter by VirF.

virB

VirF


Input: METHODS: Fifty-one healthy eyes, 169 ocular hypertensive eyes with normal visual fields, and 132 glaucomatous eyes with early visual field defects were evaluated with qualitative and quantitative measures of structural damage to the optic nerve and nerve fiber layer.


Input: Consistent with the hypothesis that it acts as transcriptional regulator, wild-type p53 protein binds DNA and activates transcription of several promoters.

wild-type p53 protein


Input: Instead, mRNA of 2. 0 and 2. 8 kb are detected in varying abundance.


Input: The sequence of the HIR1 gene predicts an 88-kDa protein with three repeats of a motif found in the G beta subunit of retinal transducin and in a yeast transcriptional repressor, Tup1.

HIR1 gene

G beta subunit

retinal transducin


Input: All of these data suggest that replication of UV-damaged templates occurs in vitro as it does in vivo and that this replication results in mutation fixation.


Input: Biol.


Input: 265, 1102-1110).


Input: The use of this model for investigating pathophysiologically significant issues in denervating diseases is illustrated with five different sets of parameters.


Input: We describe a novel cytoplasmic tyrosine kinase, termed BPK (B cell progenitor kinase), which is expressed in all stages of the B lineage and in myeloid cells.

cytoplasmic tyrosine kinase

BPK

B cell progenitor kinase


Input: However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

L-plastin


Input: Finally, we present evidence that fimbrin is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine.

fimbrin

plastin isoform


Input: The DNase I footprint extended 5' in the silencer region to include an inverted repeat of a six-nucleotide motif (epsilon-267 to-278 bp) which shares 5 of 6 bases with the GATA-1 consensus sequence.

DNase I

GATA-1 consensus sequence


Input: Furthermore, competitor containing the YY1 consensus sequence competed for protein B binding, whereas competitor containing a perfect yeast ABF-1 consensus sequence did not. (ABSTRACT TRUNCATED AT 400 WORDS)

YY1 consensus sequence

protein B

yeast ABF-1 consensus sequence


Input: Naval personnel who exceed standards for% BF can be separated from active duty.


Input: Weak promoter activity was observed for the promoter of the C1 and C2 ORFs (C1-C2 gene) and for the promoter of the V1 ORF.

V1 ORF


Input: The activity of the coat protein promoter of chloris striate mosaic virus is enhanced by its own and C1-C2 gene products.

coat protein promoter


Input: The amino acid sequence alignment of TC II with that of other Cbl binding proteins (rat intrinsic factor, human transcobalamin I and porcine haptocorrin) revealed only 33% overall homology.

TC II

Cbl binding proteins

rat intrinsic factor

human transcobalamin I

porcine haptocorrin


Input: This short communication compares a novel fluorimetric microplate enzyme immunoassay (FEIA) with a commercial time-resolved fluoroimmunoassay for the determination of thyrotropin in dried blood spots.

thyrotropin


Input: The evaluation was performed using a retrospective study design with newborn blood samples from three screening centres.


Input: When ligated to the proIL-1 beta cap site-proximal region (located between-131 to + 12), both the proIL-1 beta and the simian virus 40 enhancer elements functioned more efficiently in monocytes than in HeLa cells, which are not normally competent for IL-1 beta expression.

proIL-1 beta cap site-proximal region

proIL-1 beta

simian virus 40 enhancer elements

IL-1 beta


Input: Therefore, PC-PLC is a component of a signal transduction pathway leading to transcription of c-fos and junB that collaborates with c-myc and is independent of PKC-delta and Ras activation.

PC-PLC

c-fos

junB

c-myc

PKC-delta

Ras


Input: Southern blot analysis using probes from the 3' portions of the genomic and B. napus MA and MB cDNA clones showed that MA type myrosinases are encoded by approximately 4 genes, while MB type myrosinases are encoded by more than 10 genes in B. napus.

B. napus MA

MB cDNA

MA type myrosinases

MB type myrosinases


Input: Type II could be divided further into two forms (IIA and IIB) that may represent two underscribed species or developmental stages of the same species.


Input: In the present study we demonstrated a unique mechanism of action of 8-Cl-cAMP in the regulation of these kinase isozymes in HL-60 human promyelocytic leukemia cells.


Input: There are, however, several differences between the two new ars sequences.


Input: Pokeweed mitogen (PWM) or anti-CD3 significantly increases c-jun messenger RNA (mRNA) levels in T cells.

Pokeweed mitogen

PWM

c-jun messenger RNA (mRNA)


Input: Following seizure induction, MABP increased to 105 mm Hg and brain pHi fell to 6. 79 +/-0. 03 within 15 min and remained at this level for 1 h (P < 0. 001).


Input: Sterile mutants of Saccharomyces cerevisiae were isolated from alpha * cells having the a/alpha aar1-6 genotype (exhibiting alpha mating ability and weak a mating ability as a result of a defect in a1-alpha 2 repression).

a/alpha aar1-6

a1-alpha 2


Input: OBJECTIVE: Our purpose was to determine the simultaneous concentrations of serum cotinine in both fetal and maternal blood.


Input: 5 micrograms/l was detected in urine from some non-occupationally exposed persons.


Input: Finger associated box-zinc finger proteins (FAX-ZFPs) constitute a subfamily of the many C2H2 type ZFPs in Xenopus laevis.

Finger associated box-zinc finger proteins

FAX-ZFPs

C2H2 type ZFPs


Input: Further evidence for a clustered organization of FAX-ZFP transcription units is provided by Southern blot analysis of large genomic restriction fragments separated by transverse field gel electrophoresis, and by in situ hybridization on intact chromosomes.

FAX

ZFP


Input: Mutational analyses have demonstrated the importance of sequences within the 327 bp segment that contain a putative cyclic AMP responsive element binding protein (CREB) binding site for TGF-beta 1 and PMA responsiveness and putative PU-1 and Sp1 binding sites for basal promoter activity.

cyclic AMP responsive element binding protein

CREB

TGF-beta 1

Sp1 binding sites


Input: (1992) J.


Input: We obtained the prevalence of IgG antibodies against Trypanosoma cruzi among 1076 blood donors at the Instituto Nacional de Cardiologia " Ignacio Chavez " in Mexico City.

IgG antibodies


Input: Examination of the immediate sequence 5' to the mRNA start site reveals no TATA box and multiple known enhancer sequences.


Input: Pseudosubstrate (19-36), derived from the C-terminus of Ca-dependent PKC isotypes, inhibited beta-PKC but not nPKC activity using either Histone IIIS or peptide (19-31) as substrate.

Ca-dependent PKC isotypes

beta-PKC

nPKC

Histone IIIS


Input: This may account, at least in part, for the ability of excess wt p53 to inhibit cell proliferation and to interfere with neoplastic processes.

wt p53


Input: Northern blot analysis of total cellular RNA indicated that xylP encodes a 1. 3-kb transcript which is induced by xylan.

xylP


Input: These data show that PTH and cAMP can repress collagen promoter activity in calvariae from transgenic mice, suggesting that the alpha 1 (I) collagen promoter may contain cis elements down-stream of-2. 3 kilobases that mediate PTH and cAMP repression of collagen gene expression in bone.

PTH

collagen promoter

alpha 1 (I) collagen promoter

PTH

collagen gene


Input: The yeast gene that encodes eIF-5, designated TIF5, has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein.

eIF-5 protein


Input: Xylose-and arabinose-containing polymers were better digested than was cellulose for both breads.


Input: The alpha-subunit by itself binds to telomeric DNA.


Input: We conclude that the alpha-subunit of the telomere-binding protein, like many transcription factors, has separable DNA-binding and protein-protein interaction domains.

telomere-binding protein


Input: Calcification of a cariogenic Streptococcus and of Corynebacterium (Bacterionema) matruchotii.


Input: A comparison of the promoters for muMIP-1 beta and muMIP-1 alpha reveals a conserved CK-1 element, but transient expression studies in RAW 264. 7 macrophages with proximal fragments of either the muMIP-1 beta or the muMIP-1 alpha 5' promoter fused to a human growth hormone reporter gene link LPS-inducibility in both to promoter segments near to, but not identical with, the consensus CK-1 sequence.

muMIP-1 beta

muMIP-1 alpha

muMIP-1 beta

muMIP-1 alpha 5' promoter

human growth hormone reporter gene


Input: Sequence analysis and identification of two hyp regulatory mutants.

hyp regulatory mutants


Input: The gene encoding the receptor for macrophage colony-stimulating factor 1 (CSF-1), the c-fms protooncogene, is selectively expressed in immature and mature mononuclear phagocytes and trophoblasts.

macrophage colony-stimulating factor 1

c-fms protooncogene


Input: Since the regulation of SWI4 is required for normal cell cycle progression, we have characterized cis-and trans-acting regulators of SWI4 transcription.


Input: It also suggests that there is another pathway which can activate SWI4 transcription in the absence of SWI6.


Input: Multiple SWI6-dependent cis-acting elements control SWI4 transcription through the cell cycle.


Input: Biol.


Input: Patient characteristics associated with deep wounds as well as patient and wound characteristics predictive of the extent of healing and time required for healing were identified.


Input: Yeast mutants lacking a functional NOP1 gene can be complemented by human fibrillarin but are temperature sensitive for growth and impaired in pre-rRNA processing.

NOP1 gene

human fibrillarin


Input: The patients were divided into ischaemia and non-ischaemia groups on the basis of the change in lactate extraction ratio during balloon inflation.


Input: Both immunophilins may have important roles in receptor assembly and may represent a new category of ligand-and calcium-dependent modulators of protein function.

immunophilins


Input: Umweltchem.


Input: Contrary to expectations, we find a relatively high level of variation in the stripe 2 enhancer region, including point substitutions and insertion/deletions in binding sites, and a comparable level of variation in the other noncoding regions.

stripe 2


Input: I report here that induction of HSP82 is regulated by the early meiotic IME1-IME2 transcriptional cascade.


Input: Refugees living in Lund and repatriated to Chile considered their health as bad in a higher proportion than their Swedish counterparts, with an odds ratio of 3. 48 (2. 03-5. 66) and 4. 78 (2. 1-10. 25) respectively.


Input: This study underlines the importance of terminal SC5b-9 complement complex as a suitable marker in the evaluation of complement activation during cardiopulmonary bypass.

SC5b-9 complement complex


Input: Striking sequence similarities (57 and 53%) were observed with yeast mitochondrial proteins, SMF1 and SMF2, especially within putative functional domains: exon 6 encoding the second transmembrane spanning domain, site of the murine susceptibility mutation; and exon 11 encoding a conserved transport motif.

murine susceptibility


Input: Furthermore, we have identified a soluble form of ERp57/GRP58 by Western blotting and biosynthetic labeling.


Input: Consistent with the hepatic and epidermal expression of histidase, this finding suggests that histidase transcription may be regulated by these factors.

histidase

histidase


Input: Unlike ARF, the ARP immunoreactivity was detected in plasma membranes but not in cytosol of fractionated 3T3-L1 cells.

ARF

ARP


Input: An infectious origin should always be excluded since specific etiologic therapy may be implemented.


Input: All three NR isoforms are expressed in cv.

NR isoforms


Input: All three NR isoforms are expressed in cv.

NR


Input: The Arabidopsis FAD7 gene encodes a chloroplast omega-3 fatty acid desaturase that catalyzes the desaturation of lipid-linked dienoic fatty acids (18: 2 and 16: 2).

Arabidopsis FAD7 gene

chloroplast omega-3 fatty acid desaturase


Input: Certain transcript patterns in Epifagus plastids are highly complex and similar to those of tobacco operons.


Input: The XS2 gene down-regulates but does not abolish expression of LU genes and does not affect expression of CD44.

XS2 gene

LU genes


Input: Diabetes care: a guideline to the facilities needed to support internationally endorsed standards.


Input: The gene was uniquely mapped with odds > 1, 000: 1 on chromosome 3p in Centre d' Etude du Polymorphisme Humain pedigrees.


Input: Human LAF-4 readily hybridized with genes in mouse and chicken, thus showing that this gene family has been highly conserved during vertebrate evolution.

Human LAF-4


Input: Cycles were repeated every 3 weeks.


Input: Upstream of-37, the 5' untranslated sequences of the isolates differ in both length and sequence.


Input: SBF binds to the promoter prior to the activation of transcription in late G1, suggesting that Cln/Cdc28 kinase regulates the ability of previously bound SBF to activate transcription.

SBF

Cln

SBF


Input: However, it remains an open question whether vertebrate Hox genes expressed under the control of Drosophila regulatory sequences can substitute the function of Drosophila Hox genes.

vertebrate Hox genes

Drosophila Hox genes


Input: This approach places gHoxb-1 into the normal embryonic spatiotemporal context in which lab acts.


Input: We propose that CTS1 and CTS2 of Ci are members of two distinct classes of fungal chitinases, an observation not previously reported for a single fungus.

CTS1 and CTS2 of Ci

fungal chitinases


Input: The purpose of the study reported here was to investigate whether differences in T1 and T2 between tumors are mainly a consequence of differences in the fractional volume of the extracellular compartment.


Input: A mutant areA product truncated immediately after the last residue of the highly conserved GATA (DNA-binding) domain retains partial function.

mutant areA product

GATA (DNA-binding) domain


Input: This region contains a motif with partial identity with the binding site for the ubiquitous transcription factor upstream stimulatory factor (USF), which binds to the human insulin promoter.

upstream stimulatory factor

USF

human insulin promoter


Input: The Clb5 kinase, which promotes S phase, remains active during the G2-phase arrest of cells of the parental strain, but its activity declines rapidly in sim mutants.

Clb5 kinase

sim mutants


Input: The C/D ratio was equal to or over 0. 6 in 9 cases (16 eyes), and the values were inconsistent between both eyes in 55% of the patients.


Input: (1989), which is identical to the SSC1 gene (Smith et al., 1988).

SSC1 gene


Input: This region also contains a gene specifying a Leu-tRNA precursor and a remnant of a tau element.

Leu-tRNA precursor


Input: Occupational exposure to hepatitis B virus and human immunodeficiency virus: a comparative risk analysis.


Input: In these constructs, GUS expression was driven by promoter regions derived from the Arabidopsis alcohol dehydrogenase (Adh1), maize ubiquitin (Ubi1), rice actin (Act1) and CaMV 35S genes.

GUS

Arabidopsis alcohol dehydrogenase

maize ubiquitin

rice actin

CaMV 35S genes


Input: Its organization and regulation indicate that the S. pombe URA1 gene product appears very similar to the S. cerevisiae URA2 gene product.

S. pombe URA1 gene product

S. cerevisiae URA2 gene product


Input: (ii) opening of KATP attenuates, inhibition of the channel exacerbates functional consequences of coronary occlusion, and (iii) KATP opening attenuates reperfusion-induced VF, but it triggers ischemia-induced VF.


Input: Effects of repeated exposures of hydrogen sulphide on rat hippocampal EEG.


Input: Pseudomembranous conjunctivitis following bone marrow transplantation: immunopathological and ultrastructural study of one case.


Input: In view of the presence of SECIS elements in the open reading frames (ORFs) of bacterial selenoproteins, we examine the effects in the type 1 deiodinase of extending the ORF into the SECIS element, and find that this dramatically inhibits SECIS function.

bacterial selenoproteins

type 1 deiodinase


Input: This spindle defect of pds1 mutants results from a temperature-sensitive step that occurs around the G1/S boundary about the time of spindle assembly.

pds1 mutants


Input: In the point mutant we observed normal repair of endonuclease III site (i. e. as wild type), but no removal of CPDs at the MAT alpha and HML alpha loci.

endonuclease III site

MAT alpha

HML alpha loci


Input: However, for the evaluable cases, the performances of the CD3500 and the STKS were broadly similar and generally correlated well with the manual reference procedure.


Input: In conclusion, IgM class CIC is the predominant CIC in acute hepatitis A and correlated with disease activity.

IgM


Input: Left atrial volumes were greater in old compared with young subjects (maximal: 31 +/-10 cm3/m2 vs 24 +/-8 cm3/m2, p = 0. 02; at onset of atrial systole: 23 +/-8 cm3/m2 vs 15 +/-5 cm3/m2, p = 0. 0002; minimal: 13 +/-5 cm3/m2 vs 9 +/-4 cm3/m2, p = 0. 001).


Input: We observed abundant levels of Rev-erbA alpha mRNA in dividing C2C12 myoblasts, which were suppressed when the cells differentiated into postmitotic multinucleated myotubes.

Rev

erbA alpha


Input: We then demonstrated that 1) GAL4-REV-erbA alpha chimeras that contain the' AB' region and lack the' E' region activated transcription of GAL4 response elements in the presence of 8-Br-cAMP and 2) the ligand-binding domain (LBD) contains an active transcriptional silencer.

REV

erbA alpha

GAL4 response elements


Input: TNF Treatment of cell activated the p38 MAP kinase pathway, as revealed by increased phosphorylation of p38 MAP kinase itself, activation of the substrate protein MAPKAP kinase-2, and culminating in the phosphorylation of the heat shock protein 27 (hsp27).

TNF

p38 MAP kinase

p38 MAP kinase

MAPKAP kinase-2

heat shock protein 27


Input: However, while the sequence similarity between the membrane exons of avian mIgY and mammalian mIgG and IgE is striking, the overall similarity with Xenopus mIgY is very low.

avian mIgY

mammalian mIgG and IgE

Xenopus mIgY


Input: The novel hematopoietic growth factor FLT3 ligand (FL) is the cognate ligand for the FLT3, tyrosine kinase receptor (R), also referred to as FLK-2 and STK-1.

hematopoietic growth factor FLT3 ligand

FL

tyrosine kinase receptor

R


Input: In contrast to the selective expression of the receptor, FL expression was detected in 90-100% of the various cell types of leukemia cell lines from all hematopoietic cell lineages.

FL


Input: LY290181 appears to inhibit uPA promoter activation by blocking phorbol ester-stimulated binding of nuclear proteins to the uPA PEA3/12-0-tetradecanoylphorbol 13-acetate responsive element (TRE).

uPA promoter

uPA PEA3


Input: The protein folds correctly with two disulfide bonds and a free thiol group at Cys25.


Input: No significant differences existed between the two age groups in baseline characteristics, including treatment protocol, performance status, and serum lactate dehydrogenase (LDH) level.

serum lactate dehydrogenase

LDH


Input: Conversely, when VDR is overexpressed, vitamin D3 attenuates 9-cis RA induction from an RXR-responsive element.

VDR

RXR


Input: Mutations within conserved region 2 (CR2) of E1A that inhibit the binding of E1A to the retinoblastoma gene product (pRb) further enhanced the stimulation of transcription from the PEPCK promoter by 2 3-fold compared with wild type E1A.

retinoblastoma gene product

pRb

PEPCK promoter

wild type E1A


Input: Using autoantibodies from a Sjogren' s syndrome patient, we have previously identified a 230-kDa peripheral membrane protein associated with the cytosolic face of the trans-Golgi (Kooy, J., Toh, B.


Input: Apart from two proline-rich regions (amino acids 1-117 and 239-270), p230 contains a very high frequency of heptad repeats, characteristic of alpha-helices that form dimeric coiled-coil structures. p230 also includes the sequence ESLALEELEL (amino acids 538-546), a motif found in the granin family of acidic proteins present in secretory granules of neuroendocrine cells.

granin family


Input: They were found to stimulate at nanomolar concentrations the turnover of biosynthetically labeled ceramide, glucosylceramide, and lactosylceramide.


Input: These findings suggest that the increase in V O2 may have been a consequence of the increase in Q O2 rather than a response to the procedure itself.


Input: Consistent with ErbB-2 being a shared receptor subunit, its tyrosine phosphorylation was increased by both heterologous ligands and it mediated a trans-inhibitory effect of NDF on EGF binding.

NDF

EGF


Input: Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase.

cyclin-dependent kinase phosphorylation site

human deoxyuridine triphosphate nucleotidohydrolase


Input: VDR/RXR bound well to the VDREs and to DR4 and DR5 using the electrophoretic mobility shift assay.

VDR

RXR


Input: The sequence and isolated cDNAs will provide useful reagents for studying the expression of Brca1 in the mouse, and for testing the importance of the evolutionarily conserved domains.


Input: A study was performed to compare the ONLINE and EMIT II immunoassays with gas chromatographic/mass spectrometric (GC/MS) analysis of methaqualone metabolites on urine using samples obtained from a clinical study.


Input: Isolation by PCR of a cDNA clone from pea petals with similarity to petunia and wheat zinc finger proteins.

wheat zinc finger proteins


Input: Exposure of peripheral blood T cells from young subjects to PHA or cross-linked anti-CD3 monoclonal antibodies stimulated rapid increases in MAPK and MEK enzymatic activity.

PHA

anti-CD3 monoclonal antibodies

MAPK

MEK


Input: Deletion analysis of rOC promoter-chloramphenicol acetyltransferase constructs demonstrates that an AML-1-binding sequence within the proximal promoter (-138 to-130 nt) contributes to 75% of the level of osteocalcin gene expression.

rOC promoter

chloramphenicol acetyltransferase

AML-1-binding sequence

osteocalcin gene


Input: Escherichia coli BL21 (DE3) plysS, harbouring a recombinant plasmid containing the catalase-peroxidase gene, produced a large amount of proteins that co-migrated on SDS/PAGE with the native enzyme.

catalase

peroxidase


Input: Cdk2 formed a complex with cyclin D1 in this system.

cyclin D1


Input: The Cdk2-cyclin-D1 complex did not phosphorylate any tested substrates, such as H1 histone, pRB, SV40 large T antigen, p53, E2F-1 or a preparation of nuclear proteins from HeLa cells; in contrast, Cdk2-cyclin-E and Cdk2-cyclin-A phosphorylated these proteins.

H1 histone

pRB

SV40 large T antigen

cyclin-E

cyclin-A


Input: Cyclin-dependent kinase-2 (Cdk2) forms an inactive complex with cyclin D1 since Cdk2 associated with cyclin D1 is not phosphorylated by Cdk7-cyclin-H.

Cyclin-dependent kinase-2

cyclin D1

cyclin D1

cyclin-H


Input: A human cytoplasmic signaling protein has been cloned that possesses the same structural arrangement of SH3-SH2-SH3 domains as Grb2.


Input: Cloning of individual ZI domains upstream of a minimal promoter demonstrated that the ZIA, ZIC, and ZID domains, but not the ZIB domain, are TPA responsive.


Input: Sp1 binds two sites in the CD11c promoter in vivo specifically in myeloid cells and cooperates with AP1 to activate transcription.

CD11c promoter


Input: We found that 120 bp of the enhancer' s transcriptional core becomes DNase I hypersensitive early in B-cell development.

DNase I


Input: A developmentally modulated chromatin structure at the mouse immunoglobulin kappa 3' enhancer.

mouse immunoglobulin kappa 3' enhancer


Input: Heme oxygenase 1 is an essential enzyme in heme catabolism that cleaves heme to form biliverdin, iron, and carbon monoxide.

Heme oxygenase 1


Input: An inactive analog of wortmannin, WM12, did not affect TCR/CD3-induced Erk2 activation, and wortmannin had no effect on the activity of Erk2 when added directly to the in vitro assays.

TCR


Input: Defects in the Schizosaccharomyces pombe (Sp) cell cycle-controlling genes prevent the cell cycle progression.


Input: To determine the signal recognition particle (SRP)-SRP receptor (Srb) system in Bacillus subtilis (Bs), we cloned the Bs srb gene, which encodes a homologue of the mammalian SRP receptor alpha-subunit [Oguro et al., DNA Res.

signal recognition particle

SRP

SRP receptor

Srb

Bs srb gene

mammalian SRP receptor alpha-subunit


Input: Promoter activity was high in cell lines that expressed high levels of endogenous D3 mRNA, as indicated by Northern blot analyses, and was significantly reduced when the promoter was truncated to-122 bp.

D3 mRNA


Input: This phenomenon did not require DNA binding by the " interfering " receptor but required it to be hormone-bound, indicating that a transcriptionally active form of the interfering receptor is essential for the interfering effect.


Input: A cis-acting DNA element located between TATA box and transcription initiation site is critical in response to regulatory sequences in human angiotensinogen gene.

angiotensinogen gene


Input: The promoter of the rat PGS-2 gene contains a CAAT enhancer-binding protein consensus site (CAAT box) which can confer hormone inducibility to a PGS-2. CAT reporter gene, as well as a putative E-box region.

rat PGS-2 gene

CAAT enhancer-binding protein consensus site (CAAT box)

CAT


Input: SETTING--Delhi, urban India, 1985-6.


Input: Selective translation initiation by ribosome jumping in adenovirus-infected and heat-shocked cells.


Input: Furthermore, we have identified a 43-bp region of the 24p3 promoter required for the Dex responsiveness.

24p3 promoter


Input: Our data demonstrate directly that Rpm1r is transcribed with its substrates, tRNA met f and tRNAPro, from a promoter located upstream of the tRNA met f gene and suggest that a portion also originates from a second promoter, located between the tRNA met f gene and RPM1.

tRNA met f

tRNAPro

tRNA met f gene

tRNA met f gene


Input: Furthermore, strains with mutant RPM1 genes also accumulate precursor Rpm1r, suggesting that mutations in either gene can lead to similar biogenesis defects.

mutant RPM1 genes

precursor Rpm1r


Input: Merlie, Cold Spring Harbor Symp.


Input: Substantial evidence supports a critical role for the activation of the Raf-1/MEK/mitogen-activated protein kinase pathway in oncogenic Ras-mediated transformation.

MEK

mitogen-activated protein kinase

Ras


Input: Stylohyoid chain ossification: choice of the surgical approach.


Input: 63. 3 micromol/1, p < 0. 01) and area under the plasma concentration-time curve extrapolated to infinity AUC 9 (0-infinity) (518. 7 vs.


Input: Mean increase of milk protein yield was 46 g/d with Met plus Lys, and mean increase of true protein content was 1. 1 g/kg of milk.


Input: A chimeric VP16-Tat construct containing the leucine mutations showed no increased AP-1 responsiveness in comparison with that of the VP16 activation domain alone.

Tat

VP16 activation domain


Input: Despite significant lethality and cardiovascular dysfunction, in the septic group on Days 1 and 2, septic versus control animals had no significant differences in mean metabolic cart measured (Vo2DIR, ml/kg/min; Day 1: 11. 9 versus 12. 4, p = 0. 81; Day 2: 14. 2 versus 13. 5, p = 0. 72, respectively) and intravascular catheter calculated (Vo2INDIR, ml/kg/min; Day 1: 11. 2 versus 11. 2, p = 0. 99; Day 2: 12. 8 versus 15. 4, p = 0. 49, respectively).


Input: Although IL-2 and IFN-alpha activated STAT1 alpha and STAT5, IL-2 predominantly activated STAT5, while IFN-alpha predominantly activated STAT1 alpha.

IFN-alpha

STAT1 alpha

IFN-alpha

STAT1 alpha


Input: One complex containing a 70 D protein was found to be associated specifically with transcriptionally active leukemia cells.


Input: Our results confirm the participation of intron 1 in transcriptional regulation of the c-myb gene (in mouse and human) and implicate multiple and complex regulatory mechanisms of activation during myelomonocytic differentiation and leukemic cell growth control.

c-myb gene


Input: Limited role for PCR-based diagnosis of Whipple' s disease from peripheral blood mononuclear cells.


Input: The Pro-258--> Leu (P258L) mutation caused constitutive receptor signaling that was equivalent to about 45% of the maximum level observed in wild-type cells stimulated with alpha-factor.

alpha-factor


Input: CONCLUSIONS: Our findings suggest that pre-treatment with coenzyme Q10 may play a protective role during routine vascular procedures requiring abdominal aortic cross clamping by attenuating the degree of peroxidative damage.


Input: In a screen for genes with oncogenic potential expressed by the murine B6SUtA1 myeloid progenitor cell line, we isolated a 2.


Input: The mechanism involves Gbetagamma subunit-mediated increases in tyrosine phosphorylation of the Shc adapter protein, Shc * Grb2 complex formation, and recruitment of Ras guanine nucleotide exchange factor activity.

Gbetagamma

Shc adapter protein

Shc

Ras guanine nucleotide exchange factor


Input: The cis-acting elements that control promoter activity include binding sites for transcription factors Sp1 and alphaCbf, a 60-kDa CCAAT box-binding protein.

alphaCbf

60-kDa CCAAT box-binding protein


Input: There was a general broadening of proton resonances for a three-nucleotide segment centered about the lesion site which resulted in a tentative assignment for the sugar protons of the C7 residue in the spectrum of the adduct duplex.


Input: Using immunogold electron microscopy, we found that p58/p45 and p54 are localized on both sides of the nuclear pore complex, like p62.


Input: Transcription initiation sites of the rat II beta-, III beta, and O beta-globin genes were determined to be 52 base pairs (bp) 5'-upstream of the translation initiation codon (ATG), in each gene by primer extension analysis.

II beta-, III beta, and O beta-globin


Input: Aggravating process induced by indomethacin on chronic gastric lesion in rat.


Input: We have been studying the interaction of the oncogenic human polyomavirus BK (BKV) with the tumor-suppressor protein p53 to understand the biology of this virus as well as to understand the basic mechanisms of p53 transactivation.

tumor-suppressor protein p53


Input: Site S-II also spans a 23 bp sequence containing two tandem consensus binding sites with three base pair mismatches in each and a one base pair deletion.


Input: Unaided attempts to quit smoking are generally unsuccessful.


Input: The experience of the lateral rhinotomy approach in transsphenoidal surgery of acromegaly has been favourable.


Input: Possible relationship between hyperinsulinemia and glomerular hypertrophy in nephrosclerosis.


Input: Blood GSH-Px activity was measured with a spectrophotometer, using a modification of a previously described assay.

GSH-Px


Input: The concentrations of vitamin A precursors and vitamin E in the hay were below currently recommended dietary levels for llamas, and alfalfa hay appears to provide an unreliable source of vitamins A and E in this species.


Input: Eight cats infected with H. pylori were used in the study.


Input: However, as determined by PCR with primers specific for the 26-kDa product, the majority of cats at 2 and 4 weeks p. t. had gastric fluid samples which were positive for H. pylori and three of three cats at 2 weeks p. t. had dental plaque which was positive for H. pylori.


Input: The most frequent causes of the meningitis was the external ventricular drainage (14. 8%), post-neurosurgical (0. 8%) and head injury (0. 0007%).


Input: In vitro interaction studies, using proteins fused to glutathione-S-transferase, showed that RBP-J kappa and Su (H) bind directly to the RAM23 regions of mouse Notch1 and Drosophila Notch, respectively.

glutathione-S-transferase

RBP-J kappa

Su (H)

RAM23 regions

mouse Notch1

Drosophila Notch


Input: Twelve genomic fragments containing novel response elements are described, and the transcription unit associated with one of them, NN-84AG, was characterized in detail.


Input: Members of the Ras subfamily of small GTP-binding proteins have been shown to be promiscuous towards a variety of putative effector molecules such as the protein kinase c-Raf and the Ral-specific guanine nucleotide exchange factor (Ral-GEF).

Ras subfamily

small GTP-binding proteins

protein kinase c-Raf

Ral-specific guanine nucleotide exchange factor

Ral-GEF


Input: The cDNA encoded a mature protein of 240 amino acids, including a 29-amino acid signal sequence.


Input: Biol.


Input: The effect of ligustrazine hydrochloride (LTH) on depressing pulmonary artery hypertension has been proved in recent studies.


Input: In the same period we pointed out an increase of hematocrit (from 29% to 35%) and of the Hb (from 9. 3 to 11. 2 g/dl).

Hb


Input: The structure of these genes suggests a common ancestor for all viperid PLA2 genes.

viperid PLA2 genes


Input: Chloroplast mutator (chm) of Arabidopsis is a recessive nuclear mutation that causes green and white variegation in leaves and is inherited in a non-Mendelian fashion.

Chloroplast mutator

chm


Input: Also like the thrombin receptor, PAR2 can be activated by the hexapeptide corresponding to its tethered ligand sequence independent of receptor cleavage.

thrombin receptor


Input: Such an increase in the steady-state testicular TIMP-2 mRNA level apparently is not the result of an up-regulation by germ cells because germ cells cocultured with Sertoli cells failed to elicit an increase in the Sertoli cell steady-state TIMP-2 mRNA level.

testicular TIMP-2 mRNA

TIMP-2 mRNA


Input: In the SPK and PTA category, anti-T-cell therapy significantly lowered the risk of graft loss.


Input: Five-year survivals amounted to 100%, 86. 2%, 59. 4%, 29. 8%, and 20% for stages I, II, III, IVA and IVB respectively.


Input: Targeted disruption of the OGG1 gene in yeast revealed a second OG glycosylase/lyase protein, tentatively named Ogg2, which differs from Ogg1 in that it preferentially acts on OG: G.

OGG1 gene

OG glycosylase/lyase protein


Input: Sequence analysis of the isolated genomic clone revealed that the DNA binding domain of this orphan receptor is most homologous to the human TR2 receptor.

human TR2 receptor


Input: Genomic DNA hybridization suggests that SpSHR2 is a single-copy gene in the S. purpuratus genome.


Input: Its hydropathy profile predicts seven transmembrane spans and a hydrophilic amino terminus of approximately 100 residues, and it suggests the presence of an amphiphilic alpha-helix (L-61 to F-97) in close proximity to the first strongly hydrophobic segment of ProW.

ProW


Input: There are four polyglutamine motifs interspersed with histidine-rich regions.


Input: Quantitative analysis of plasmid loss rates in cdc28-1N strains carrying plasmids with multiple replication origins suggests that a defect in initiating DNA replication probably causes this plasmid loss phenotype.


Input: Platelet-derived growth factor-dependent activation of phosphatidylinositol 3-kinase is regulated by receptor binding of SH2-domain-containing proteins which influence Ras activity.

Platelet-derived growth factor

phosphatidylinositol 3-kinase

SH2-domain-containing proteins

Ras


Input: Almost complete sequence identity was found between the 3' end of the DXS6673E gene and two expressed sequence tags (ESTs) and between the 5' end of the DXS6673E gene and a third EST.

DXS6673E gene

DXS6673E gene


Input: Several of the exon boundaries correspond to the boundaries of functional domains in the p55 protein.

p55 protein


Input: Tristetraprolin (TTP) is the prototype of a group of potential transcription factors that contain two or more unusual CCCH zinc fingers.

Tristetraprolin

TTP


Input: The ewes were returned to normoxia, and monitoring was continued for 1 h.


Input: Feline leukemia viruses (FeLVs), which are replication-competent oncoretroviruses of the domestic cat species, are contagiously transmitted in natural environments.


Input: Both verapamil and propranolol can exhibit additive effects with adenosine in prolonging AV nodal conduction time; however, only verapamil can reduce the dose of adenosine required to produce AV nodal block.


Input: RVR' loss of function' studies by constitutive over-expression of a dominant negative RVR delta E resulted in increased levels of p21Cip1/Waf1 and myogenin mRNAs after serum withdrawal.

RVR

RVR delta E

myogenin mRNAs


Input: These two repeat motifs are organized in an extremely well-ordered pattern in each domain, which suggests that SbHRGP3 belongs to a new group of proteins having the repeat motifs of two distinct groups of dicot extensins.

dicot extensins


Input: Two classes of inactive receptors were identified: one in which both transcriptional activation and dimerization were compromised and a second in which only transcriptional activation was abolished.


Input: Here we localize a transferable 40-amino acid region within the LBDs of RXR, RAR, TR, and chicken ovalbumin upstream promoter transcription factor that is critical for determining identity in the heterodimeric interaction and for high-affinity DNA binding.

LBDs

RXR

RAR

TR

chicken ovalbumin upstream promoter transcription factor


Input: OKA and calyculin A do not decrease OCFRE DNA-protein interactions, suggesting that important protein-protein interactions are phosphatase regulated.

OCFRE


Input: IMPLICATIONS: Given the small size of this study and the inconsistency of results among the few prospective studies of ovarian cancer conducted to test these associations, replications of these findings are highly desirable.


Input: We have performed toeprinting analyses on repA mRNA of plasmid R1, both free and in duplex with the antisense RNA, CopA.

repA mRNA

CopA


Input: Previous work showed that repA (initiator protein) expression requires tap (leader peptide) translation.

repA

tap


Input: Tonsillectomy is an effective means of prophylaxis for upper respiratory infection in habitual angina patients.


Input: These results were corroborated in a subsequent study in which 30 hypogonadal men were supplemented with SLT 5 mg three times daily for 6 months.


Input: The elevations achieved by LDEE given s. c. were higher than those achieved after i. p. administration and lasted for longer periods.


Input: Many human viruses are able to develop suitable strategies for modifying apoptosis in virus-infected cells and in virus-primed T cells.


Input: Taken together, these observations indicate that the C-terminal 24 amino acids are sufficient for NodO secretion although the region adjacent to this domain appears to affect secretion efficiency.

NodO


Input: The model also predicts that blood flow shunt fraction (Qs/QT) is directly related to the oxygen sine-wave amplitude perturbations transmitted to end-expired air and arterial and mixed-venous blood through two simple equations.


Input: Discrimination between HIV-1 and HIV-2 showed evidence for the presence of HIV-1 only.


Input: However, an intrinsic DNA-binding subunit for HiNF-D was not identified.

HiNF-D


Input: The HiNF-D (CDP/cut) complex with the H4 promoter is immunoreactive with antibodies against CDP/cut and pRB but not p107, whereas the CDP/cut complex with a nonhistone promoter (gp91-phox) reacts only with CDP and p107 antibodies.

HiNF-D

CDP

cut

H4 promoter

CDP

cut

pRB

CDP

cut

phox

CDP


Input: Eight-four ACOAs were tested in order to examine the fit of the model to the data by path analysis with LISREL VII.


Input: Using anchored PCR, a VL element rearranged to CL2 was isolated.

VL element


Input: A subset of these DMP1 recognition sequences containing a GGA trinucleotide core can also function as Ets-responsive elements.

DMP1 recognition sequences

Ets-responsive elements


Input: In both settings, it can be phosphorylated by cyclin D-dependent kinases, suggesting that its transcriptional activity may normally be regulated through such mechanisms.

cyclin D-dependent kinases


Input: Enterococcus faecium strains with vanA-mediated glycopeptide resistance were isolated by enrichment culture from the intestines and feces of several animal species, mainly horses and dogs (8% positive), chickens (7% positive), and pigs (6% positive).


Input: Oncogenic activation of the tyrosine kinase domain of the human trk proto-oncogene by fusion to a cell adhesion molecule.

tyrosine kinase domain

human trk proto-oncogene


Input: Physiol.


Input: They suggest that decreased steady-state levels of IME1 and IME2 mRNA were not merely the result of non-specific adverse affects on nucleic acid metabolism caused by the yvh1 disruption.

IME2 mRNA


Input: RNase protection analysis of two rRNA fragments whose genes are adjacent provided evidence for a polycistronic transcript containing sequences from both, as well as separate small RNAs.

RNase


Input: These two contigs contain a total of 163 open reading frames (ORFs) in 26-29 putative operons; 56 ORFs could be identified with reasonable certainty.


Input: The evidence in support of this was derived from the fact that the affinity or interaction between the two subunits was impaired as indicated by the first order rate constant of hCG alpha 1 beta (km = 4. 1 x 10 (-2) min-1) at pH 3. 0 at 23 degrees C which is one order of magnitude greater relative to rehCG (kw = 4. 6 x 10 (-3) min-1).

hCG alpha 1 beta

rehCG


Input: Rifabutin has substantial efficacy when combined with other agents.


Input: Leukemia-inhibitory factor (LIF) is a neuropoietin able to regulate the differentiation and the survival of many cell types, which include some neuronal populations.

Leukemia-inhibitory factor

LIF

neuropoietin


Input: Paracrine activation of the HIV-1 LTR promoter by the viral Tat protein is mechanistically similar to trans-activation within a cell.

HIV-1 LTR promoter

viral Tat protein


Input: RUSH-1 beta is a 95-kDa truncated version of RUSH-1 alpha that results from alternative splicing of a 57-bp exon as confirmed by genomic cloning.

RUSH-1 beta

RUSH-1 alpha


Input: The mobility shift assay of the 65 bp (-318/-254) fragment with nuclear extract from the dark-adapted sample showed an additional band, not seen with the light-grown sample.


Input: Moreover, the same mutations alter the structure of junB 5' flanking DNA within chromatin.

junB


Input: RESULTS: The age distribution was 28 to 83 years old (mean was 54. 1 years).


Input: The ERH expression profile is similar, to that of An3, which localizes to the animal hemisphere of oocytes and is abundantly expressed in the embryo.

ERH


Input: When the degree of exercise was maximal, mPAP was maintained, SVI decreased, HR was unchanged, and CO and VO2 decreased.


Input: 327 and 736 protocols of postmortem examinations from Moscow hospitals N 31 and 57, respectively, were evaluated statistically.


Input: The effect of six arginine mutations of oxidative phosphorylation and AAC expression.

AAC


Input: The RI alpha gene is composed of nine coding exons of varying lengths, separated by introns, giving the gene a total length of at least 21 kb. our recent cloning of a processed RI alpha pseudogene with a 5'-noncoding region different from the previously reported RI alpha complementary RNA indicated that the RI alpha gene may have multiple leader exons giving rise to alternately spliced messenger RNAs (mRNAs).

RI alpha gene

RI alpha pseudogene

RI alpha complementary RNA

RI alpha gene


Input: This establishes Rad3/Mec1 as the only conserved protein which is required for all the DNA structure checkpoints in both yeast model systems.


Input: The identification of the hepatitis C virus as the major cause of liver disease both in dialysis patients and in transplant patients has focused attention on the epidemiology and the impact of continuing infection in these patient groups.


Input: Stable transfection of the BL cell line Raji with constructs containing core promoter mutations confirmed that the proximal Sp1 site and the TATA box are essential for the activation of promoter P1 by the Ig kappa enhancers.

Sp1 site

Ig kappa enhancers


Input: The first identification of the active 37LRP/p40 gene presented in this study is a critical step toward the isolation of the corresponding human gene and the understanding of the molecular mechanisms involved in the up-regulation of its expression during tumor invasion and metastasis.


Input: The promoter region (P1) corresponding to the main group of transcription initiation sites is devoid of TATA and CAAT boxes but has putative binding sites for the transcription factor SP1 and is embedded in a large G + C-rich domain of a CpG island, features shared by the promoters of constitutively expressed housekeeping genes.

transcription factor SP1


Input: The STAT-1 signaling pathway provides at least one mechanism for activation of the CAEV LTR by IFN-gamma in monocytes.

CAEV LTR

IFN-gamma


Input: Unlike wild-type p53, the delta proAE mutant cDNA can be stably expressed in tumor derived cell lines with few immediate detrimental effects.

wild-type p53

delta proAE mutant cDNA


Input: Several artifacts occurred that interfered with visualization of the diaphragm.


Input: During skeletal muscle development, different types of muscle fibers are generated, which express different combinations of muscle-specific gene products.


Input: Its transcription product, a 1. 3 kb mRNA, is polyadenylated at a site containing consensus eukaryotic polyadenylation signals and mapping 87 bp downstream of the translation termination codon for CG30.


Input: BCL-2, an inhibitor of apoptosis in a wide variety of cell types, has been reported to prevent oxidative stress-induced cell death.


Input: Full-length AT-PHH1, and both AT-PHH1 and AT-PHH1 delta C-513 (truncated to be approximately the size of microbial photolyase genes) cDNAs, were overexpressed, respectively, in yeast and Escherichia coli mutants hypersensitive to ultraviolet light.

Full-length AT-PHH1

AT-PHH1 delta C-513

microbial photolyase genes


Input: Serotonin concentration in the blood of patients with hemorrhagic fever with renal syndrome


Input: Control examination was performed at the end of each period.


Input: The total number of specimens was 131 with 78 nonsmokers and 53 smokers.


Input: In Experiment 1, pups that had received an injection of the noncompetitive N-methyl-D-aspartate receptor antagonist MK-801 (0. 1 mg/kg, i. p.) either 30 min before or immediately after conditioning spent less time over the conditioned odor than saline-treated controls.

N-methyl-D-aspartate receptor


Input: Serotonin receptors in suicide victims with major depression.

Serotonin receptors


Input: Experimental data showed that these abnormal proteins are constitutively localized in the nucleus, have lost the transcriptional repressor functions typical of normal NF-kappa B2p52 and may be capable of transactivation activity.

NF-kappa B2p52


Input: Because L-plastin expression in tissue-specifically regulated in both humans and rodents, it is likely that similar mechanisms regulate L-plastin gene expression in human and rodent cells and that they could be identified by comparing the function and nucleotide sequences of the human and murine L-plastin gene promoters.

L-plastin

L-plastin gene

L-plastin


Input: How does a nurse go about maintaining her level of competence when she is one of the few local practitioners in her field? Vancouver sex therapist Bianca Rucker is doing it by cultivating a network of colleagues and mentors in related fields both at home and across the continent.


Input: Finally, antibody binding to site IIIa on the hCG-ectodomain complex was also hindered by an anti-peptide mAb directed against a peptide encoded by the eighth exon (pE x 8) of the LHR.

hCG-ectodomain complex

LHR


Input: In rlf2 mutants, telomeric chromatin is perturbed: Telomeric silencing is reduced and Rap1p localization is altered.

rlf2 mutants


Input: This 0. 74 kb cDNA contains an open reading frame (ORF) of 477 bp encoding a polypeptide of 159 amino acids (aa) which differs at only one position (position 65) from the human U1-C protein.

human U1-C protein


Input: However, the action of each cognate ligand and the accessory cellular factors that can differentially regulate the transcriptional responses of a heterodimer-DNA complex are not well understood.


Input: We have measured the MBF on incisors and its direction in three dimensions for different jaw openings in ten subjects.


Input: Clinically meaningful decreases due to alkalinization alone within 30 minutes are unlikely.


Input: In co-transfection studies, an AP-2 but not an Egr-1 expression vector activated VPF/VEGF transcription, thus indicating that AP-2 protein is functionally important in TGF alpha-induced VPF/VEGF gene expression.

VPF

VEGF

AP-2 protein

TGF alpha

VPF

VEGF


Input: Cytohesin-1, a protein abundant in cells of the immune system, has been proposed to be a human homolog of the Saccharomyces cerevisiae Sec7 gene product, which is crucial in protein transport.

Saccharomyces cerevisiae Sec7 gene product


Input: In this regard, it differs from a recently reported BFA-sensitive ARF-GEP that contains a Sec7 domain.

ARF

GEP

Sec7 domain


Input: The recombinant protein was purified to homogeneity by (NH4) 2SO4-precipitation and affinity chromatography on 5' AMP-Sepharose.


Input: Derepression of gene expression mediated by the 5' upstream region of the isocitrate lyase gene of Candida tropicalis is controlled by two distinct regulatory pathways in Saccharomyces cerevisiae.

isocitrate lyase gene of Candida tropicalis


Input: 99mTc-HMPAO was distributed in the territories of the ACA and MCA in the two patients who were treated with intraarterial infusion of papaverine from the C4 segment, but was distributed only to the territory of the ACA in four patients who were treated with intraarterial infusion of papaverine from the C1 segment at 1 ml/min.


Input: This brief review analyses these interactions and defines clinical settings where antibiotic-induced endotoxin release may prove to be clinically relevant.


Input: These changes in virus entry features may result in coronaviruses with novel pathogenic properties.


Input: Effect of Azadirachta indica hydroalcoholic leaf extract on the cardiovascular system.


Input: In cellular supernatant fraction, SHRSP showed a decrease of magnesium in many tissues and an elevation of the calcium to magnesium ratio when compared to age-matched WKY and SHRSR.


Input: The reduced NO production in these cells was associated with low levels of mRNA of inducible NO synthetase.

inducible NO synthetase


Input: Apart from iron regulation, sodA expression was affected by changes in DNA topology induced by coumermycin A but not by the global virulence regulatory Bvg system.

sodA

Bvg system


Input: In the present study, we have isolated and sequenced several p15E cDNA gene fragments amplified by means of polymerase chain reaction (PCR) from parental (P815) and xenogenized (P815/DTIC) tumour cells.

p15E cDNA gene fragments


Input: Transcriptional activity was measured by slot-blot hybridization with steady-state RNA isolated from lacZ + M. smegmatis clones.

lacZ + M. smegmatis


Input: Movement time and kinematic characteristics were analyzed together with the magnitude of cerebral blood flow to identify areas of brain activity proportionate to task and movement variables.


Input: Areas with significantly greater rCBF for targeting were the left motor cortex, left intraparietal sulcus, and left caudate.


Input: Recent studies have demonstrated that the U1 snRNP is recruited to the 5' splice site by protein/protein interactions involving the SR domains of the U1-70K protein and SF2/ASF.

U1 snRNP

SR

U1-70K protein

ASF


Input: Thus, the tri-snRNP-specific 27K protein could potentially be involved in SR protein-mediated protein/protein interactions and, additionally, its phosphorylation state could modulate pre-mRNA splicing.

SR


Input: Although methods to align the control and activation fMR images may correct for some of this motional error, they will be incomplete in correcting for those that depend on spatial orientation.


Input: Three women have had abnormal endocervical follow up cytology suggestive of residual disease.


Input: This site is upstream from the TATA box used in somatic cells.


Input: An aromatic stacking interaction between subunits helps mediate DNA sequence specificity: operator site discrimination by phage lambda cI repressor.

phage lambda cI repressor


Input: We have characterized a panel of 6 monoclonal antibodies raised against human platelet talin by Western blotting, immune precipitation, and immunofluorescence, and shown that antibodies TA205 and TD77 disrupt actin stress fibers and focal adhesions, and inhibit cell motility when microinjected into human fibroblasts.

human platelet talin

antibodies TA205

actin


Input: Here we report the purification of this larger form as an approximately 320-kDa particle that contains mRNP3 + 4 and nine additional polypeptides, including mRNA-binding polypeptides of 34 and 36 kDa and a doublet of 110/105 kDa that proved to be nucleolin.

mRNP3 + 4

nucleolin


Input: This study indicates that the phenotype of myofibrillar disarray seen in HCM patients which harbor either of these two mutations may not be directly due to the failure of the mutant myosin heavy chain protein to assemble and form normal sarcomeres, but may rather be a secondary effect possibly resulting from the chronic stress of decreased beta MHC function.

mutant myosin heavy chain protein

beta MHC


Input: A method for the simultaneous determination of de (N-methyl)-N-ethyl-8, 9-anhydroerythromycin A 6, 9-hemiacetal (EM523, I) and its three metabolites in human plasma and urine has been developed using high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection.


Input: Cotransfection of Ets-2 and p44 mitogen-activated protein (MAP) kinase expression vectors strongly potentiated HB-EGF promoter activation in response to deltaRaf-1: ER.

p44 mitogen-activated protein (MAP) kinase

HB

EGF

ER


Input: The Rlm1 protein, a member of the MADS box family of transcription factors, functions downstream of Mpk1 in the pathway.

Rlm1 protein

MADS box family


Input: The likelihood that a common region of deletions would contain a tumor suppressor is strongly enhanced by coincidence of that region with a chromosome fragment suppressing tumorigenicity upon introduction in tumor cells.


Input: The carboxyl-terminal CCCC module is structurally related to the DNA-binding domain of the erythroid transcription factor GATA-1.

erythroid transcription factor GATA-1


Input: The different holoenzyme RNA polymerases generated upon reconstituting these mutants independently with core RNA polymerase (alpha2beta beta') have shown reduced transcriptional activity in comparison to the enzyme containing wild-type sigma factor.

holoenzyme RNA polymerases

core RNA polymerase

alpha2beta beta'

wild-type sigma factor


Input: These data are consistent with a model in which GATA-5 performs a unique temporally and spatially restricted function in the embryonic heart and lung.


Input: The similar phenotypes of bur6 and bur3 (mot1) mutations suggest that Bur6p and Mot1p have related, but not identical, functions in modulating the activity of the general transcription machinery in vivo.


Input: A genetic screen applied to mutants in the branch site region shows that all positions in the conserved TACTAAC sequence are important for intron recognition.


Input: Implication of PAF and acetylhydrolase (PAF-AH) activity in periodontal disease.

PAF

acetylhydrolase

PAF-AH


Input: A polypeptide encoded by the NTS 16 open reading frame has sequence similarity to the catalytic domain of several receptor protein kinases from plants including the S-receptor kinases implicated in the rejection of self-pollen in Brassica species and the Pto gene product of tomato which confers resistance to a bacterial pathogen.

receptor protein kinases from plants

S-receptor kinases

Pto gene product of tomato


Input: The atp 1 and atp 2 types of cDNA sequences were the most redundant among the 28 different isoperoxidases identified among about 200 peroxidase encoding ESTs.

atp 1

atp 2

isoperoxidases

peroxidase encoding ESTs


Input: Glycemic response to malted, popped and roller dried wheat-legume based foods in normal subjects.


Input: 4. 26 +/-1. 54 mmol/l, P < 0. 01), and systolic BP responses to intravenous norepinephrine and angiotensin II were significantly higher on glibenclamide than on metformin (P < 0. 02 and P < 0. 05, respectively).

angiotensin II


Input: Is leishmaniasis endemic on the island of Minorca (Spain)? A human visceral case after living 13 years in Minorca.


Input: RESULTS: Surprisingly, PAF blockade increased mortality after trauma (5 of 11 WEB-2086 animals versus 1 of 9 vehicle animals; p = 0. 15) and depressed cardiac index and O2 delivery at 72 hours (p < 0. 05).

PAF


Input: This finding is the first example of utilization of noncomplementary dinucleotide primer by an RNA polymerase.

RNA polymerase


Input: Pure T1 and dual-T1 images were visually evaluated for image quality (IQ) on a five-point scale (0 = unacceptable to 4 = excellent).


Input: This conformer features a Type I beta-turn and has extensive hydrophobic contacts with the FKBP12 binding surface.


Input: Children' s temperament and maternal socialization at Time 1 (n = 103, aged 2-3 years) were considered predictors of future conscience, assessed using new observational and narrative measures.


Input: One P1 genomic clone and six subsequent plasmid subclones were isolated and analyzed for the exon-intron organization of the Ctpct gene.

Ctpct gene


Input: Congruent with empirical predictions, the most homesick children perceived low control over homesickness and separation, and coped by relinquishing control.


Input: We also review the role of grains in the formation of complex molecules in interstellar molecular clouds.


Input: The C-LIP also was compared with transcather arterial embolization (TAE; C-LIP followed by gelatin sponge) and percutaneous ethanol injection therapy (PEIT).


Input: We also demonstrate that preformed triplexes are quite stable when precipitated with ethanol and resuspended in water.


Input: Transfection experiments using preformed triplexes with a reporter plasmid containing the collagen promoter sequence showed significant inhibition of transcription when compared with a control phosphorothioate ODN.

collagen promoter sequence


Input: Thus, an increase in plasma TBG and a shift from adrenal androgen and mineralocorticoid steroid secretion towards cortisol secretion may be endocrine markers for progression of the disease in patients with HIV-infection.

plasma TBG


Input: Latanoprost produces an additional reduction of intraocular pressure (IOP) when used in combination with timolol, pilocarpine, acetazolamide and dipivefrin.


Input: Transient overexpression of c-Jun induced tenascin-C expression in primary REF and in FR3T3, an established fibroblast cell line.

c-Jun

tenascin-C


Input: Selected parameters: heart rate (HR), arterial blood pressure (ABP) and arterial O2 saturation (SaO2) monitored by pulsoximetry were measured during the procedure.


Input: We have tested this proposal by carrying out circular dichroism (CD) and NMR experiments on the Skn domain and five truncated proteins.

Skn domain


Input: Genes for ocs element binding factors (OBFs), belonging to a specific class of basic-region leucine zipper (bZIP) transcription factors, have been isolated in a number of plants.

ocs element binding factors

OBFs

basic-region leucine zipper (bZIP) transcription factors


Input: SRE activity is dependent upon the activation by phosphorylation of a ternary complex factor; included among the ternary complex factors is Elk-1.


Input: A xylE transcriptional fusion to the putative mxbD promoter showed low-level expression in wild-type cells grown on one-carbon (C1) compounds and no detectable expression in cells grown on succinate.

xylE transcriptional fusion

mxbD promoter


Input: Pax-3 is a paired-type homeobox gene that is specifically expressed in the dorsal and posterior neural tube.

paired-type homeobox gene


Input: Instead, they contained dendritic cells that express melanogenic marker proteins such as tyrosinase and tyrosinase-related protein 1.

tyrosinase

tyrosinase-related protein 1


Input: These mutations create stop codons in exon 7 and 8, respectively, and probably result in truncated proteins lacking HLH-Zip or Zip structure.


Input: These six amino acids are part of an amphipathic helix that is highly conserved among nuclear hormone receptors and contains the core domain of the ligand-dependent transactivation function, AF-2.

nuclear hormone receptors


Input: Surprisingly, the RXR-specific ligand 9-cis-retinoic acid induced binding of SRC-1 to the RXR component of the TRE-bound heterodimer.

RXR

RXR component


Input: Using PRL-R tagged both in the N-terminal or C-terminal regions of the mature receptor excludes the possibility of a cleaved fragment which could have been subsequently imported into the nucleus.

PRL-R


Input: Transcription of these genes was also elevated in gut and lung during freezing, but mRNA levels in these tissues were lower than in liver.


Input: The most important one among them is Cyclosporin A, which is a selective immunosuppressive drug.


Input: Using a conditional-lethal mutant allele of SUP45 (sup45-2) and a combination of in vivo and in vitro approaches, we demonstrate that the product of the SUP45 gene (Sup45p or eRF1) is a factor required for translation termination in yeast.

SUP45 gene


Input: She was able to stand unaided by 3 years of age with then progressive worsening of motor abilities.


Input: Merosin positive congenital muscular dystrophy with mental deficiency, epilepsy and MRI changes in the cerebral white matter.

Merosin


Input: Also, transient overexpression of this protein in C2C12 cells reduced the transcription of a CAT-reporter regulated by an E12/MyoD driven enhancer.

CAT

MyoD


Input: Type I position-vestibular-pause (PVP I) and vestibular-only (V I) neurons, as well as a smaller number of other type I and type II eye-plus-vestibular neurons were studied.


Input: Using mouse-human somatic hybrids and FISH analysis, the PE-2 gene is localized to human chromosome 19q13. 2, a region involved in translocations and deletions in leukemias and several solid tumors, suggesting that this novel ETS factor may play a role in carcinogenesis.

PE-2 gene

ETS factor


Input: Sequence divergence is observed in untranslated regions which allows the definition of gene-specific probes.


Input: In adherent macrophages, absence of CD45 led to the hyperphosphorylation and hyperactivation of p56/59 (hck) and p53/56 (lyn), but not of p58 (c-fgr).

hck

lyn

c-fgr


Input: Secretion in milk and transplacental transfer of two iodized oils, Lipiodol UF and Oriodol, in rabbits.


Input: The RNA-binding and RNA-DNA helicase activities of the Escherichia coli transcription termination factor rho have been investigated using natural RNA molecules that are 255 and 391 nucleotide residues in length and that contain the trp t' rho-dependent termination sequence of E. coli.

RNA-DNA helicase

Escherichia coli transcription termination factor rho

trp t' rho-dependent termination sequence


Input: The serum levels of beta-human chorionic gonadotropin (HCG) and placental alkaline phosphatase (PLAP) were not elevated.

beta-human chorionic gonadotropin

HCG

placental alkaline phosphatase

PLAP


Input: Thus E14. 1TG3B1 is a useful ES cell line for modifying the mouse genome using the HPRT gene as a selection marker and for transmission at a high frequency into the mouse germ line.

HPRT gene


Input: OBJECTIVE: This study was designed to analyze the common cause of asthenopia, mixed astigmatism.


Input: One RNA construct which consisted of 112 nucleotides (nt) from nt 639 to nt 750 formed a heterodimeric complex with the RNA which consisted of 200 nucleotides from nt 551 to nt 750.


Input: However, we could not find the homologous regions with guanine nucleotide exchange factors or GTPase-activating proteins in the c-cbl gene.

guanine nucleotide exchange factors

GTPase-activating proteins

c-cbl gene


Input: Aryl hydrocarbon receptor nuclear translocator (ARNT) is a component of the transcription factors, aryl hydrocarbon receptor (AhR) and hypoxia-inducible factor 1, which transactivate their target genes, such as CYP1A1 and erythropoietin, in response to xenobiotic aromatic hydrocarbons and to low O2 concentration, respectively.

Aryl hydrocarbon receptor nuclear translocator

ARNT

aryl hydrocarbon receptor

AhR

hypoxia-inducible factor 1

erythropoietin


Input: We also provide evidence that gar2 is phosphorylated in vitro by a p13 (suc1)-Sepharose-bound kinase from Schizosaccharomyces pombe extracts that displays cell cycle-regulated activity similar to that of the p34 (cdc2 (kinase.


Input: However, by immobilized metal affinity chromatography assay, self-association of PR-A was 3. 5-fold more efficient than that of either the DhLBD or hLBD constructs.

PR-A


Input: The overall results of this paper are consistent with the conclusion that the carboxyl-terminal LBD is not sufficient for mediating PR dimerization and that multiple regions, including the hinge and amino-terminal sequences, contribute either directly or indirectly to homodimerization of PR.

PR

PR


Input: Colorectal carcinoma: therapeutic approach in patients already treated with metastasis resection


Input: Alternative splicing of ClC-6 (a member of the CIC chloride-channel family) transcripts generates three truncated isoforms one of which, ClC-6c, is kidney-specific.

CIC chloride-channel family


Input: The frequency of positive anti-GM1 antibody titers in the Guillain-Barre syndrome patients with PEN 19 isolates was higher than that in the Guillain-Barre syndrome and Fisher' s syndrome patients without PEN 19 isolates.

anti-GM1 antibody


Input: NF-kappa B/Rel transcription factors participate in the activation of numerous genes involved in immune regulation/inflammation including cytokines, cell surface receptors, adhesion molecules, and acute phase proteins.

NF-kappa B

Rel


Input: However, mean food intake in the 40% group was half that of the ACT group (p < 0. 001) and significantly less (p < 0. 01) than the SEP group, which consumed amounts equivalent to 65% of daily requirement.


Input: We have separated a dermatan sulfate proteoglycan, epiphycan, from decorin and biglycan by using dissociative extraction of bovine fetal epiphyseal cartilage, followed by sequential ion-exchange, gel permeation, hydrophobic, and Zn2 + chelate chromatographic steps.

decorin


Input: The mean times to detection of all mycobacteria with BACTEC 9000 MB and BACTEC 460 TB were similar (10. 3 and 10. 0 days, respectively).


Input: Though the BACTEC 9000 MB system is recommended for respiratory specimens, we demonstrated that it can be successfully used also for recovery of mycobacteria from clinical specimens from various extrapulmonary sites.


Input: A pre-boutonniere deformity was simulated by dividing the central slip.


Input: This regulation requires two HMG-box proteins: the ubiquitous Ste11 transcription factor and the M cell-controlling protein Mat1-Mc.

HMG-box proteins

Ste11 transcription factor


Input: Neither of these proteins, individually or as a pair, can bind the alpha-globin 3' UTR unless they are complexed with the remaining non-poly (C) binding proteins of the alpha-complex.

alpha-globin 3' UTR

alpha-complex


Input: (1996a) Biochemistry 35, 1589-1598].


Input: A prospective observational study was conducted to identify early indicators of acute dengue virus infection.


Input: It is present in the nucleus of the cells in which it is expressed and can phosphorylate and activate the cyclic AMP response element binding proteins CREB and CREM tau in a manner analogous to protein kinase A.

cyclic AMP response element binding proteins CREB

CREM tau

protein kinase A


Input: These cells fail to generate the signals to phosphorylate CREB and produce significantly less of the cytokine Interleukin-2 (IL-2) in response to agents that either increase intracellular Ca2 + and/or activate protein kinase C.

CREB

protein kinase C


Input: Both of these dogs had low serum IgG (3. 5 to 7. 2 mg/ml) and the second littermate also had reduced serum IgA (< 0. 1 to 0. 15 mg/ml).

IgG

IgA


Input: CONCLUSIONS: Valproate appears to inhibit the glucuronidation of carbamazepine-10, 11-trans-diol, and probably also inhibits the conversion of carbamazepine-10, 11-epoxide to this trans-diol derivative, rather than simply inhibiting the latter reaction only.


Input: Proteinuria in a young man.


Input: Neural blockade in chronic and cancer pain.


Input: Five cell strains of human dermal fibroblasts were each treated with three samples of burn blister fluid and the effect compared with the rate of contraction of free floating fibroblast populated collagen lattices (FPCL).

collagen


Input: It is thus one of the key enzymes involved in the triiodothyronine-mediated control of growth, differentiation and basal metabolism in vertebrates.


Input: We had previously analyzed repair rates of cyclobutane pyrimidine dimers at nucleotide resolution along the human JUN gene in normal fibroblasts and found very efficient repair of sequences near the transcription initiation site but slow repair along the promoter.

human JUN gene


Input: The used expression system could allow to produce mutated forms of SsEF-2 obtained by mutagenesis of the corresponding gene.


Input: Crosstalk among the pathways may explain how some forms of stress can contribute to the development of a malignancy.


Input: MEASUREMENTS AND MAIN RESULTS: Lung elastance (EL) and resistance (RL) were calculated from measurements of airway pressure, esophageal pressure, and airway flow in five anesthetized, paralyzed dogs during sinusoidal forcing at a constant mean airway pressure of 10 cmH2O in a wide range of breathing frequencies (0. 2 to 1. 0 Hz in intervals of 0. 2) and tidal volumes (50, 100, 200, and to 300 mL).


Input: Because the biosynthetic pathway to the vacuole intersects with the endocytic pathway, internalization of a bulk membrane endocytic marker FM 4-64 was assayed in the sop mutants.

sop mutants


Input: Naltrexone has been recently approved by the Food and Drug Administration for the treatment of alcohol dependence.


Input: Consistent with this model, a synthetic construct containing three tandem copies of the native LDL receptor SREBP site linked to a single Sp1 site was also significantly activated in a buttonhead-independent fashion.

LDL receptor SREBP

Sp1 site

buttonhead


Input: Analysis of the 5' flanking region of the gene also revealed the presence of multiple TATA and CAAT sequences.


Input: Analysis of the 5' flanking region of the gene also revealed the presence of multiple TATA and CAAT sequences.


Input: The m7GpppN cap structure of eukaryotic mRNA is formed cotranscriptionally by the sequential action of three enzymes: RNA triphosphatase, RNA guanylyltransferase, and RNA (guanine-7)-methyltransferase.

RNA triphosphatase

RNA guanylyltransferase

RNA (guanine-7)-methyltransferase


Input: CONCLUSION: Patients wit clinically palpable neck disease (N1-3), histological evidence of metastatic nodal disease, extracapsular spread, and three or more positive lymph nodes are at greater risk of developing failure at distant sites.


Input: Area under the drug concentration-time curves (AUC0-24 hr) for MTX were 2379 and 3534 ng * hr/ml from PG-2. 5% Azone and PG-7. 5% Azone systems respectively.


Input: There were differences between males (p < 0. 05) for most of the characteristics studied.


Input: Although previous data have suggested that Rev uses the same export pathway as uracil-rich small nuclear RNAs and 5S ribosomal RNA, the CTE seems to interact with evolutionarily conserved factors that are essential for cellular mRNA export.

Rev

5S ribosomal RNA


Input: S2F, a leaf-specific trans-acting factor, binds to a novel cis-acting element and differentially activates the RPL21 gene.

RPL21 gene


Input: Polarised expression of human intestinal N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase (human meprin) alpha and beta subunits in Madin-Darby canine kidney cells.

N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase

human meprin


Input: Neither mutant exhibited derepression of the silent mating type loci.

silent mating type loci


Input: In contrast to the wild-type protein, expression of p45 NF-E2 lacking this activation domain in an NF-E2 null cell line fails to support enhancer-dependent transcription in transient assays.

p45 NF-E2


Input: These findings suggest one potential mechanism for direct recruitment of distal regulatory regions of the globin loci to the individual promoters.

globin loci


Input: The promoter region showed no consensus TATA box but it contains CCAAT and CreA boxes known to be involved in both stress and carbon-catabolite regulation of fungal promoters.

CreA


Input: In support of this interpretation we demonstrate that MQ9b binds strongly 5 of 17 motif-positive, pathogen-derived synthetic peptides.


Input: These discoloration' s can be treated in several ways but up to lately tooth structure had to be removed in an irreversible manner in order to provide sufficient bulk for the new restorative material.


Input: Overexpression of BAG-1 also protected certain cell lines from heat shock-induced cell death.


Input: The 5' flanking region contains potential binding sites for TATA-binding protein, Sp1, nuclear factor 1 (NF1), CAAT-box binding protein (C/EBP), hepatocyte nuclear factors 1 and 5 (HNF1, HNF5) and activator proteins 1 and 2 (AP1, AP2).

TATA-binding protein

nuclear factor 1

CAAT-box binding protein

C/EBP

hepatocyte nuclear factors 1 and 5

activator proteins 1 and 2


Input: The recognition specificity of the p55 PDZ domain appears to be unique, since the three PDZ domains of hDlg (human lymphocyte homologue of the Drosophila discs large tumor suppressor) do not bind the cytoplasmic domain of glycophorin C.

p55 PDZ domain

PDZ domains

hDlg

Drosophila discs large tumor suppressor

glycophorin C


Input: In the United States high-MW HES 480 which is difficult to degrade is most frequently used and results in a larger in vivo MW and subsequent decrease in factor VIII/von Willebrand factor levels.

factor VIII

von Willebrand factor


Input: In our previous studies, transcriptional activation was shown to correlate with IEP86 binding to both the TATA-box binding protein (TBP) and the transcription factor bound upstream.

TATA-box binding protein

TBP


Input: Previously, we reported that scanthrough translation, where the initiating AUG of a primary open reading frame is bypassed, is most likely to account for the presentation of cryptic epitopes from alternative reading frames within the influenza A PR/8/34 nucleoprotein gene.

influenza A PR/8/34 nucleoprotein gene


Input: The deduced amino acid sequence of the gene has significant homology to the interferon regulatory factors (IRFs).

interferon regulatory factors

IRFs


Input: The pheromone response pathway activates transcription of Ty5 retrotransposons located within silent chromatin of Saccharomyces cerevisiae.

Ty5 retrotransposons


Input: The malate synthase gene, MLS1, of the yeast Saccharomyces cerevisiae is transcriptionally regulated by the carbon source in the growth medium.

malate synthase gene


Input: By deletion analysis of the MLS1 control region, we identified two sites, UAS1 and UAS2, as important for efficient derepression of the gene.


Input: Prior to meals 2 to 3 times daily, 1-2 tablespoons of Alzoon are recommended.


Input: These results suggest that UBP41 may play an important role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin chains generated by the action of 26 S proteasome on poly-ubiquitinated protein substrates, as well as in the production of free ubiquitin from linear poly-ubiquitin chains and of certain ribosomal proteins from ubiquitin fusion proteins.

ubiquitin

branched poly-ubiquitin chains

26 S proteasome

ubiquitinated

linear poly-ubiquitin chains

ubiquitin fusion proteins


Input: The regurgitation of large vitreous injections.


Input: Attenuation from the vit A2 consensus ERE is not necessarily dependent on DNA binding as the TR alpha DNA binding mutant was still able to inhibit E-dependent transactivation.

vit A2

TR alpha


Input: Null mutations in daf-3 suppress mutations in genes encoding this TGF-beta signal, its receptors, and associated Smad signal transduction proteins. daf-3 encodes a Smad protein that is most closely related to mammalian DPC4, and is expressed throughout development in many of the tissues that are remodeled during dauer development.

TGF-beta

Smad signal transduction proteins

Smad protein

mammalian DPC4


Input: We have overexpressed, purified, characterized, and crystallized the BTB/POZ domain from PLZF (PLZF-BTB/POZ).

BTB

PLZF

PLZF

BTB


Input: Furthermore, deletion and mutation analyses of the VCAM-1 promoter performed with chloramphenicol acetyltransferase constructs revealed that Tax was trans activating the VCAM-1 promoter via two NF-kappaB sites present at bp-72 and-57 in the VCAM-1 gene promoter, with both of them being required for the Tax-induced expression of this adhesion molecule.

VCAM-1 promoter

chloramphenicol acetyltransferase constructs

Tax

VCAM-1 promoter

NF-kappaB sites

VCAM-1 gene promoter

Tax


Input: The AGM and NIH/Swiss mouse CCR5 proteins are 97. 7 to 98. 3% and 79. 8% identical to the human protein, respectively.

AGM and NIH/Swiss mouse CCR5 proteins


Input: Recently, roles also have been suggested for the nuclear trans-factor GATA-1 in regulating progenitor cell proliferation.


Input: (ii) An AF G-CSF level > 2000 pg/ml is a strong positive predictor of CAM.

G-CSF


Input: [Pulmonary vascular resistance (PVR) during 10% O2-PVR during 21% O2/PVR during 21% O2] x 100 was termed as hypoxic pulmonary vasoconstriction (HPV).


Input: An unusual cysteine triplet conserved in the sequences of TB domains is localized to the hydrophobic core, at the C-terminus of an alpha-helix.

TB domains


Input: Protein phosphatases play a critical role in the regulation of the eukaryotic cell cycle and signal transduction.


Input: Re: " Assessing the direction of causality in cross-sectional studies ".


Input: Restriction enzyme mapping, subcloning, and DNA sequencing analysis of recombinant phage lambda and P1 clones revealed that exons encoding the 1. 9-kb mouse TS mRNA are dispersed over > 150 kb genomic DNA.

mouse TS mRNA


Input: Transfection analyses indicated that the expression of Tbxas1 is controlled by a short (70-bp) positive regulatory sequence and several upstream repressive elements.


Input: Electromobility shift and cotransfection assays demonstrated that HNF1alpha, but not HNF4, bound to its cognate site and transactivated G6Pase gene expression.

G6Pase gene


Input: Differential regulation of the pre-C and pregenomic promoters of human hepatitis B virus by members of the nuclear receptor superfamily.

pre-C

nuclear receptor superfamily


Input: These results implicate a precursor-specific base-paired structure involving sequences on both sides of the mature cleavage site in the 3' processing of human U2 RNA.

human U2 RNA


Input: The mouse M-lysozyme downstream enhancer has been previously characterized on several levels of gene regulation.

mouse M-lysozyme downstream enhancer


Input: Bitter cassava contains cyanogenic glycosides; processing breaks them down to acetone cyanohydrin and hydrogen cyanide.


Input: First, two MCTs using a long electrode, 3 cm in size, were performed in the central area of the tumor, and eight MCTs by a short electrode, 2 cm in size, were done in the peripheral and surrounding area of the tumor.


Input: We also show by immunogold electron microscopy immunocytochemistry that amphiphysin I is localized in the nerve terminal cytomatrix and is partially associated with endocytic intermediates.

amphiphysin I


Input: The prescription for apoplexy includes C1-7, T1-9 and L2-4.


Input: Thus, dpp and omb promote both dorsal leg cell fate as well as transdetermination-competent leg disc cells.

dpp

omb


Input: These observations provide strong support for the idea that expression of mutant tRNA can confer a mutator phenotype, including the UVM-constitutive phenotype observed in mutA and mutC cells.

mutA

mutC


Input: We now demonstrate that CCAAT/enhancer-binding protein (C/EBP) delta is a major component of a PGE2-stimulated DNA-protein complex involving HS3D and find that C/EBPdelta transactivates IGF-I promoter 1 through this site.

CCAAT/enhancer-binding protein (C/EBP) delta

C/EBPdelta

IGF-I promoter 1


Input: Interleukin-1 levels remained low throughout the course.


Input: Southern blot analysis of endonuclease-digested genomic DNA from primary chick embryo fibroblasts (CEF) suggested that MEK2 is a single-copy gene in this vertebrate species.


Input: SCID V (D) J recombination can be partly rescued in T-lymphocytes by either DNA-damaging agents (gamma-irradiation and bieomycin) or a null mutation of the p53 gene, possibly because of transiently elevated DNA repair activity in response to DNA damage or to delayed apoptosis in the absence of p53.

V

D

J

p53 gene


Input: The existence of these two categories of strains offers a new genetic system in which the properties of a potential invertebrate retrovirus can be tested.


Input: TSA treatment, however, did not detectably alter enhancer factor binding or the positioning of nuc-1 on the majority of the chromatin templates indicating that protein acetylation and chromatin remodeling may be limiting steps that occur only on transcriptionally competent templates, or that remodeling of nuc-1 requires additional factors.


Input: Y15170 (Surf-2, Surf-4), Y15171 (Surf-3, Surf-1, Surf-6), and Y15172 (Surf-5.)]


Input: The mean values of the first three components were not significantly different in ARVD patients and control subjects.


Input: However, the signaling cascade utilized by the urokinase receptor is only incompletely understood.

urokinase receptor


Input: The interaction between DDB and E2F1 can also be detected by coimmunoprecipitation experiments.

DDB


Input: Lysine-ketoglutarate reductase and saccharopine dehydrogenase from Arabidopsis thaliana: nucleotide sequence and characterization.

Lysine-ketoglutarate reductase

saccharopine dehydrogenase


Input: The pel gene from an Amycolata sp. encoding a pectate lyase (EC 4. 2. 2. 2) was isolated by activity screening a genomic DNA library in Streptomyces lividans TK24.

pel gene

pectate lyase

EC 4. 2. 2. 2


Input: In the clinical study, the defect size shown by BMIPP imaging was greater in anterior than in inferior infarcts (p < 0. 001) and correlated well with the risk area revealed by contrast ventriculography (r = 0. 80, p < 0. 0001) CONCLUSION: The above preliminary data, admittedly from a small group of patients, suggest that tomographic BMIPP imaging provides an accurate quantification of defect size by means of a simple threshold technique and, in the subacute phase, permits determination of the amount of myocardium at risk after acute myocardial infarction.


Input: The promoter for HMG-CoA synthase contains two binding sites for the sterol regulatory element-binding proteins (SREBPs).

HMG-CoA synthase

sterol regulatory element-binding proteins

SREBPs


Input: Although RAD17, RAD24 and MEC3 are not required for cell cycle arrest when S phase is inhibited by hydroxyurea (HU), they do contribute to the viability of yeast cells grown in the presence of HU, possibly because they are required for the repair of HU-induced DNA damage.


Input: Protein C deficiency or protein S deficiency was the only identified risk factor for 5. 4% (2 patients) and 13. 5% (5 patients), respectively, of these 37 children.

Protein C

protein S


Input: A reciprocal binding assay using IM-9 cells as a source of SHP-1 and SHP-2 revealed specific association of SHP-2 (but not SHP-1) with a glutathione S-transferase fusion incorporating GHR cytoplasmic domain residues 485-620, but only if the fusion was first rendered tyrosine-phosphorylated.

glutathione S-transferase fusion

GHR cytoplasmic domain residues 485-620


Input: We have used mutation-directed chemical cross-linking with bis (sulfosuccinimidyl) suberate (BS3) to investigate the architecture of the gp41 oligomer.

gp41 oligomer


Input: Consistent with previous reports, addition of a myristoylation signal to mSos1 (MyrSos1) rendered it transforming for NIH 3T3 cells and deletion of the mSos C terminus (MyrSos1-deltaC) did not interfere with this activity.

mSos C terminus


Input: Demonstration of tissue lesions after intramuscular injection by determination of creatine kinase in blood

creatine kinase


Input: Levels of the MEK inhibitor PD98059 that block EGF-induced mitogenesis and MAP kinase phosphorylation also abrogate EGF-induced focal adhesion disassembly and cell motility.

MEK

EGF

MAP kinase

EGF


Input: Surprisingly, the COOH-terminal domain of RanGAP1 was also found to harbor a nuclear localization signal.


Input: Here, we present evidence that exposure of DT40 lymphoma B cells to low energy electromagnetic field (EMF) results in a tyrosine kinase-dependent activation of phospholipase Cgamma2 (PLC-gamma2) leading to increased inositol phospholipid turnover.

tyrosine kinase

phospholipase Cgamma2


Input: PATIENTS AND METHOD: Since 1986, 62 patients were irradiated stereotactically.


Input: Training for audit.


Input: Cytoskeletal polarization of T cells is regulated by an immunoreceptor tyrosine-based activation motif-dependent mechanism.


Input: Here we have examined the ability of the cellular protein YB-1 to modulate transcription of the HIV-1 promoter in a human astrocytic cell line (U-87MG), a neuronal cell line (SK-N-MC) and lymphoid cells (Jurkat) by transfection assay.

HIV-1 promoter


Input: Substrates for p210 (bcr-abl) are likely to be involved in the pathogenesis of CML.

bcr

abl


Input: The COOH-terminal region of the transcripts contained fifteen triplet repeats (GCT; alanine) at nucleotide 465 to 509, which is significantly expanded compared to the rat RL14.

rat RL14


Input: Control of fatty liver syndrome in a Jersey herd by a change of diet and the use of recombinant bovine somatotrophin.

somatotrophin


Input: During organogenesis, HFH-8 expression is found in the splanchnic mesoderm in close apposition of the gut endoderm, suggesting a role in mesenchymal-epithelial induction of lung and gut morphogenesis.


Input: However, laparoscopy failed to establish inoperability in any cases of carcinoma spread to areas not accessible to laparoscopic visualization.


Input: No difference in telomere length was seen in mutants affected in the regulation of Cdc2, whereas some of the DNA repair mutants examined had slightly longer telomeres than did the wild type.


Input: Sp1 can activate transcription through immunoglobulin kappa-chain enhancer or P-selectin promoter NF-kappaB sites. p50 homodimers replace Sp1 from the P-selectin promoter by binding site competition and thereby either inhibit basal Sp1-driven expression or, in concert with Bcl-3, stimulate expression.

immunoglobulin kappa-chain enhancer

P-selectin promoter NF-kappaB sites

p50 homodimers

P-selectin promoter


Input: For the 5HT5A receptor the addition of yohimbine resulted in a similar but smaller effect.

5HT5A receptor


Input: CONCLUSIONS: XCoe2 may play a pivotal role in the transcriptional cascade that specifies primary neurons in Xenopus embryos: by maintaining Delta-Notch signalling, XCoe2 stabilises the higher neural potential of selected progenitor cells that express X-ngnr-1, ensuring the transition between neural competence and irreversible commitment to a neural fate; and it promotes neuronal differentiation by activating XNeuroD expression, directly or indirectly.

Delta

Notch

XNeuroD


Input: RESULTS: Patients in Group A had a higher incidence of posterolateral wall motion abnormalities (p < 0. 001) on radionuclide ventriculography, a larger infarct area (as evidenced by higher peak creatine kinase levels) (p < 0. 02) and a lower left ventricular ejection fraction (LVEF) at hospital discharge (p < 0. 008) than those in Group B.

creatine kinase


Input: In all cells, the accumulation of high Dsg protein levels required calcium and was not observed in low calcium (0. 05-0. 07 mM) media.

Dsg protein


Input: RESULTS: Using information in the dbEST database of expressed sequence tags, we isolated an Arabidopsis thaliana gene (GCR1) that encodes a protein with seven predicted membrane-spanning domains and other features characteristic of 7TM receptors.

7TM receptors


Input: E1A represses apolipoprotein AI enhancer activity in liver cells through a pRb-and CBP-independent pathway.

apolipoprotein AI enhancer

pRb

CBP


Input: M-66 identified the 14-kDa protein in another MMTV bearing T-cell lymphoma, EL-4.


Input: RT-PCR indicated that p21 mRNA was induced 1. 4-, 2. 0-, and 3. 1-fold in the 2-day neonatal, 7-day neonatal, and adult stages, respectively, compared to the 17-day fetal stage.

p21 mRNA


Input: Ganciclovir and foscarnet efficacy in AIDS-related CMV polyradiculopathy.


Input: BACKGROUND AND OBJECTIVES: The sexually transmitted diseases (STD) control program for female sex workers (FSW) in Lima, Peru, provided periodic serological tests for syphilis and cervical smears for gonococci, but not medication for STD or condoms.


Input: In order to examine the potential role of transcriptional silencing during productive HSV-1 infection, recombinant viruses were generated in which wild-type or mutant ICP34. 5 promoters controlling the expression of a chloramphenicol acetyltransferase reporter gene were inserted into the thymidine kinase gene of the viral genome.

wild-type or mutant ICP34. 5 promoters

chloramphenicol acetyltransferase reporter gene

thymidine kinase gene


Input: Members of the meis1 and pbx homeodomain protein families cooperatively bind a cAMP-responsive sequence (CRS1) from bovine CYP17.

pbx homeodomain protein families

bovine CYP17


Input: The RAS-cyclic AMP-dependent protein kinase cAPK pathway prevents the UAS activity of IREu in the presence of glucose as the sole carbon source, while the transcriptional activators Msn2p and Msn4p promote the UAS activity of this repeat in the presence of acetate.

RAS

cyclic AMP-dependent protein kinase cAPK

IREu


Input: Alternative splicing of fibroblast growth factor receptor 2 (FGF-R2) is an example of highly regulated alternative splicing in which exons IIIb and IIIc are utilized in a mutually exclusive manner in different cell types.

fibroblast growth factor receptor 2


Input: SNAC or PBS was infused for 6. 5 h, beginning 30 min before ischemia and continuing throughout the duration of reperfusion.


Input: Moreover, it is the assumptions behind steady-state O2 uptake that do not permit proper interpretation of energy expenditure during EPOC; 1 l O2 not = 20. 9 kJ.


Input: The predicted amino acid sequence of m-Staf is highly homologous to that of Staf, another selenocysteine tRNA gene transcription activating factor of Xenopus laevis.

m-Staf

Staf

selenocysteine tRNA gene transcription activating factor


Input: Recent studies have revealed unconventional myosin V to be an important actin-based molecular motor involved in vesicular movement.

myosin V

actin


Input: Furthermore, a minor start site was localized 179 bp upstream of the major site using reverse transcriptase-polymerase chain reaction with various P1 primers (primer walking), primer extension, and cDNA cloning.

reverse transcriptase