Datasets:
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license: unknown
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---
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license: unknown
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tags:
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- biology
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- rna
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- genomics
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---
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# Overview
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eCLIP is an experimental protocol which measures the in vivo binding sites of RNA-binding proteins (RBPs) across the transcriptome by coupling ultraviolet crosslinking, immunoprecipitation, and high-throughput sequencing. In this dataset, the eCLIP tracks have been reprocessed using [Peakhood](https://github.com/BackofenLab/Peakhood) to report the number of RBP-specific binding sites across two cell lines.
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This dataset is redistributed as part of mRNABench: https://github.com/morrislab/mRNABench
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# Data Format
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Description of data columns:
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- target_{rbp}: Number of binding sites in transcript for specific RBP.
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- cds: binary track which reports position of first nucleotide in each codon in CDS.
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- splice: binary track which reports position of the 3' end of each exon, indicating splice sites.
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# Data Source
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This dataset is generated using raw eCLIP data generated by the Yeo lab as part of the ENCODE project: https://www.encodeproject.org/eclip/. (See ENCODE [data use policy](https://www.encodeproject.org/help/citing-encode/))
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Please attribute:
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ENCODE paper: https://www.nature.com/articles/nature11247
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eCLIP Protocol: https://www.nature.com/articles/nmeth.3810
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Original dataset source: https://www.encodeproject.org/
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Citations:
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- The ENCODE Project Consortium. An integrated encyclopedia of DNA elements in the human genome. Nature 489, 57–74 (2012). https://doi.org/10.1038/nature11247
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- Van Nostrand, E., Pratt, G., Shishkin, A. et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nat Methods 13, 508–514 (2016). https://doi.org/10.1038/nmeth.3810
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