import scanpy as sc import pandas as pd from matplotlib.pyplot import rc_context from matplotlib.colors import LinearSegmentedColormap from dataloader import load_singlecell_data,getEntrezGeneSymbol from pathlib import Path def umap_plot(input_data_key,input_data_value): EntrezGeneSymbol = getEntrezGeneSymbol(input_data_key,input_data_value) BASE_PATH = Path(__file__).parent single_cell_data_path = str(BASE_PATH.parent / "Core data") single_cell_data=load_singlecell_data(single_cell_data_path) color_vars=['leiden',EntrezGeneSymbol] # Define colour map as requested by the client (from grey to blue) cmap = LinearSegmentedColormap.from_list("grey_to_blue", ["#d3d3d3", "blue"]) with rc_context({"figure.figsize": (6, 6)}): sc.pl.umap(single_cell_data, color=color_vars, # plot UMAP cmap=cmap, # /* Reference 1 - taken from https://scanpy.readthedocs.io/en/stable/tutorials/plotting/core.html */ legend_loc="on data", # Place labels on the data frameon=True, legend_fontsize=4.5, legend_fontoutline=1,) # /* end of reference 1 */ return sc.pl # umap_plot('EntrezGeneSymbol','THBS1') # EXAMPLE OF USAGE WITH GENESYMBOL # umap_plot('EntrezGeneID','7057')