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Mycobacterium marinum infections in humans and tracing of its possible environmental sources.
|
The low frequency of nontuberculous mycobacterial infections, nonspecific symptoms for individual mycobacteria, and the lack of specific identification methods could alter correct diagnosis. This study presents a combined microbiology and molecular-based approach for Mycobacterium marinum detection in four aquarists with cutaneous mycobacterial infection. Simultaneously, ecology screening for M. marinum presence in the aquarists' fish tanks was performed. A total of 38 mycobacterial isolates originated from four human patients (n = 20), aquarium animals (n = 8), and an aquarium environment (n = 10). Isolate identification was carried out using 16S rRNA sequence analysis. A microbiology-based approach, followed by 16S rRNA sequence analysis, was successfully used for detection of M. marinum in all four patients. Animal and environmental samples were simultaneously examined, and a total of seven mycobacterial species were isolated: Mycobacterium chelonae , Mycobacterium fortuitum , Mycobacterium gordonae , Mycobacterium kansasii , Mycobacterium mantenii , Mycobacterium marinum , and Mycobacterium peregrinum . The presence of M. marinum was proven in the aquarium environments of two patients. Although M. marinum is described as being present in water, it was detected only in fish.
|
['Animals', 'Anti-Bacterial Agents', 'Clarithromycin', 'Environmental Microbiology', 'Female', 'Fishes', 'Humans', 'Male', 'Microbial Sensitivity Tests', 'Middle Aged', 'Mycobacterium', 'Mycobacterium Infections, Nontuberculous', 'Mycobacterium marinum', 'RNA, Ribosomal, 16S', 'Treatment Outcome']
| 22,182,182
|
[['B01.050'], ['D27.505.954.122.085'], ['D02.540.576.500.992.100'], ['H01.158.273.540.274', 'N06.850.425'], ['B01.050.150.900.493'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.875.595', 'E05.200.875.595', 'E05.337.550.400'], ['M01.060.116.630'], ['B03.510.024.962.500', 'B03.510.460.400.410.552.552'], ['C01.150.252.410.040.552.475'], ['B03.510.024.962.500.720.500', 'B03.510.460.400.410.552.552.720.500'], ['D13.444.735.686.670'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]']
| 0
| 1
| 1
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|
Prophylactic antipyretic treatment with acetaminophen in acute ischemic stroke: a pilot study.
|
Fever is associated with poor outcome in acute stroke. Forty-two consecutive, normothermic patients with acute ischemic stroke were, within 24 hours from symptom onset, randomized to either receive 4 g acetaminophen daily (n = 20) or matched placebo (n = 22). Fever of greater than 37.5 degrees C occurred in 36.4% of patients in the placebo group, compared with 5.0% in the acetaminophen group (Fisher's exact test, p = 0.014). Prophylactic antipyretic treatment with acetaminophen may be effective in the prevention of fever after acute ischemic stroke.
|
['Acetaminophen', 'Adult', 'Analgesics, Non-Narcotic', 'Brain Ischemia', 'Double-Blind Method', 'Female', 'Humans', 'Male', 'Stroke']
| 11,756,615
|
[['D02.065.199.092.040', 'D02.092.146.113.092.040'], ['M01.060.116'], ['D27.505.696.663.850.014.040', 'D27.505.954.427.040.100'], ['C10.228.140.300.150', 'C14.907.253.092'], ['E05.318.370.300', 'E05.581.500.300', 'N05.715.360.325.320', 'N06.850.520.445.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.140.300.775', 'C14.907.253.855']]
|
['Chemicals and Drugs [D]', 'Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Multimerization of the Drosophila zeste protein is required for efficient DNA binding.
|
The Drosophila zeste protein forms multimeric species in vitro through its C-terminal domain. Multimerization is required for efficient binding to DNA containing multiple recognition sequences and increasing the number of binding sites stimulates binding in a cooperative manner. Mutants that can only form dimers still bind to a dimeric site, but with lower affinity. Mutations or progressive deletions from the C-terminal show that when even dimer formation is prevented, DNA-binding activity is lost. Surprisingly, binding activity is regained with larger deletions that leave only the DNA-binding domain. Additional protein sequences apparently inhibit DNA binding unless they permit multimerization. The DNA-binding domain peptides bind strongly even to isolated recognition sequences and they bind as monomers. The ability of various zeste peptides to stimulate white gene expression in vivo shows that multimeric forms are the functional species of the zeste product in vivo. The DNA-binding domain peptide binds well to DNA in vitro, but it cannot stimulate white gene expression in vivo. This failure may reflect the need for an activation domain or it may be caused by indiscriminate binding of this peptide to non-functional isolated sites. Multimerization increases binding specificity, selecting only sites with multiple recognition sequences.
|
['ATP-Binding Cassette Transporters', 'Amino Acid Sequence', 'Animals', 'Base Sequence', 'Binding Sites', 'DNA', 'DNA-Binding Proteins', 'Drosophila', 'Drosophila Proteins', 'Eye Color', 'Eye Proteins', 'Insect Hormones', 'Leucine Zippers', 'Molecular Sequence Data', 'Peptides', 'Promoter Regions, Genetic', 'Protein Conformation']
| 8,491,197
|
[['D12.776.157.530.100', 'D12.776.395.550.020', 'D12.776.543.550.192', 'D12.776.543.585.100'], ['G02.111.570.060', 'L01.453.245.667.060'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['G02.111.570.120'], ['D13.444.308'], ['D12.776.260'], ['B01.050.500.131.617.720.500.500.750.310.250'], ['D12.776.093.500.462'], ['G14.340', 'G16.690.360'], ['D12.776.306'], ['D06.472.445.573'], ['G02.111.570.820.709.275.500.520'], ['L01.453.245.667'], ['D12.644'], ['G02.111.570.080.689.675', 'G05.360.080.689.675', 'G05.360.340.024.340.137.750.680'], ['G02.111.570.820.709']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
[The surgical treatments and clinical characteristics of snoring with epiglottis collapse].
|
Objective:The aim of this study is to summary the characteristics of sleep breath disorder patients with epiglottis collapse and find out the way to treat them with surgery.Method:This is a retrospective study. There were eighteen patients who complained of snoring and somnolence, as well as had undergone DISE. Eight of them were diagnosed as epiglottis collapse. We analysis the clinical features, surgical treatments and prognosis of these eight patents, and compare their BMI, shape of epiglottis and AHI with the patients without epiglottis collapse. Result: Patients with epiglottis collapse usually complained of breathless during sleeping. The shape of their epiglottis was mainly flat and wide, as the cure of the upper margin of their epiglottises shows significant difference with those who are without epiglottis collapse(P=0.03,0.04).DISE is the diagnosis examination. The primary surgical treatment is Epiglottoplasty. Conclusion: It's not uncommon to see epiglottis collapse clinically. Whenever we meet the particular clinical manifestation,the diagnose of epiglottis collapse should be taken into consideration. DISE is the diagnosis examination. Epiglottoplasty can treat them to some degree.
|
['Endoscopy', 'Epiglottis', 'Humans', 'Polysomnography', 'Retrospective Studies', 'Sleep Apnea, Obstructive', 'Snoring']
| 29,986,561
|
[['E01.370.388.250', 'E04.502.250'], ['A02.165.257.625.411', 'A02.165.407.500.411', 'A04.329.591.411'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.520.625'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['C08.618.085.852.850', 'C10.886.425.800.750.850'], ['C23.888.852.779.850']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Formation and release of nitric oxide from human neutrophils and HL-60 cells induced by a chemotactic peptide, platelet activating factor and leukotriene B4.
|
Vascular endothelial cells and neutrophils synthesize and release potent vasodilatatory factors, i.e. endothelium-derived relaxing factors (EDRF) and neutrophil-derived relaxing factors (NDRF). One EDRF has been identified as nitric oxide (NO) derived from arginine. We studied the synthesis and release of NO from human neutrophils stimulated with the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine, platelet activating factor or leukotriene B4. The formation and release of NO was enhanced several-fold in the presence of superoxide dismutase, probably by inhibiting superoxide-induced breakdown of NO. The formation and release of NO but not the formation of superoxide anions was decreased in neutrophils pretreated with L-canavanine, an inhibitor of arginine-utilizing enzymes. Our data suggest that at least one NDRF is identical with NO or another labile NO containing compound derived from arginine.
|
['Bucladesine', 'Calcitriol', 'Canavanine', 'Cell Line', 'Humans', 'In Vitro Techniques', 'Kinetics', 'Leukemia, Promyelocytic, Acute', 'Leukotriene B4', 'Luminescence', 'N-Formylmethionine Leucyl-Phenylalanine', 'Neutrophils', 'Nitric Oxide', 'Platelet Activating Factor']
| 2,537,760
|
[['D03.633.100.759.646.138.395.250', 'D13.695.462.200.250', 'D13.695.667.138.395.250', 'D13.695.827.068.395.250'], ['D04.210.500.247.222.159.478.387.300', 'D04.210.500.247.808.146.478.387.300', 'D04.210.500.812.768.196.478.387.300', 'D10.570.938.146.478.387.300'], ['D12.125.311'], ['A11.251.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.481'], ['G01.374.661', 'G02.111.490'], ['C04.557.337.539.275.700'], ['D10.251.355.255.100.450.411', 'D10.251.355.310.166.887.411', 'D23.469.050.175.450.415'], ['G01.358.500.505.650.665', 'G01.590.540.665', 'G01.750.250.650.665', 'G01.750.770.578.665'], ['D02.886.030.676.450.440', 'D12.125.072.050.685.445', 'D12.125.142.666.500', 'D12.125.166.676.450.440', 'D12.644.456.400', 'D23.125.685'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['D02.033.100.291.211.500', 'D02.092.063.291.211.500', 'D02.092.877.883.333.710', 'D02.675.276.232.710', 'D10.570.755.375.760.400.985.910', 'D23.119.865', 'D23.469.050.600']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
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The expression of cyclin G in nasopharyngeal carcinoma and its significance.
|
To investigate the expression of cyclin G1, cyclin G2 in nasopharyngeal carcinoma (NPC) cell lines and its significance. The protein expression of cyclin G1, cyclin G2 in NPC cell lines of different differentiation degree (HNE2, CNE1) was detected by indirect immunofluorescence. The mRNA expression of cyclin G1, cyclin G2 in HNE2 and CNE1 was measured with RT-PCR. The cyclin G1 expression in HNE2 and CNE1 was weak, and cyclin G2 expression in the cytoplasm near cell membrane was strong, continuous, and homogeneous. The expression level of cyclin G1-mRNA in HNE2 was 2.097 ± 0.262, which was significantly higher than CNE1 (0.997 ± 0.286, P < 0.05); the expression level of cyclin G2-mRNA in HNE2 was 0.708 ± 0.107, which was significantly lower than CNE1 (1.216 ± 0.037, P < 0.05). Abnormal expression of cyclin G was closely related to tumor differentiation, the origin, and progression of NPC.
|
['Carcinoma', 'Cell Differentiation', 'Cell Line, Tumor', 'Cell Membrane', 'Cell Nucleus', 'Cyclin G1', 'Cyclin G2', 'Cytoplasm', 'Disease Progression', 'Fluorescent Antibody Technique', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Nasopharyngeal Carcinoma', 'Nasopharyngeal Neoplasms', 'RNA, Messenger', 'Reverse Transcriptase Polymerase Chain Reaction']
| 21,688,120
|
[['C04.557.470.200'], ['G04.152'], ['A11.251.210.190', 'A11.251.860.180'], ['A11.284.149'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['D12.644.360.262.200.100', 'D12.776.167.218.200.100', 'D12.776.476.262.200.100'], ['D12.644.360.262.200.200', 'D12.776.167.218.200.200', 'D12.776.476.262.200.200'], ['A11.284.430.214'], ['C23.550.291.656'], ['E01.370.225.500.607.512.240', 'E01.370.225.750.551.512.240', 'E05.200.500.607.512.240', 'E05.200.750.551.512.240', 'E05.478.583.375'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.557.470.200.623', 'C04.588.443.665.710.650.500', 'C07.550.350.650.500', 'C07.550.745.650.500', 'C09.647.710.650.500', 'C09.775.350.650.500', 'C09.775.549.650.500'], ['C04.588.443.665.710.650', 'C07.550.350.650', 'C07.550.745.650', 'C09.647.710.650', 'C09.775.350.650', 'C09.775.549.650'], ['D13.444.735.544'], ['E05.393.620.500.725']]
|
['Diseases [C]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Mental illness in a rural area: a Norwegian psychiatric epidemiological study.
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OBJECTIVES: Few epidemiological studies have compared less well-integrated urban areas with well-integrated rural areas with the same methods. The aim of this study was to explore the prevalence of mental disorder in a socially stable demographic western region of Norway and make comparison with previously observed prevalence figures of mental illness in Oslo, the capital of Norway.METHOD: A random sample of the 107,738 residents of Sogn and Fjordane, a western rural region of Norway, age 18-65 years, was drawn from the Norwegian Population Register. A total of 1,080 subjects, 63% of the original sample, were interviewed with the Composite International Diagnostic Interview.RESULTS: The mean age of the subjects was 39.2 years. The 12-month prevalence of mental illness was 16.5% and the lifetime prevalence was 30.9%. Simple phobia and social phobia had the highest 12-month prevalence whereas alcohol abuse and major depression had the highest lifetime prevalence. All mental disorders were more prevalent in women than in men, with the exception of alcohol and drug abuse. Severe psychopathology was found in 2.2% (12 month prevalence) and 5.1% (lifetime prevalence). These observations show that the 12-month and the lifetime prevalence of mental illness in this western area is approximately half the rate of figures observed for Oslo.CONCLUSION: Epidemiological figures for a western rural region of Norway showing 12-month and the lifetime prevalence of mental disorder are considerably lower than figures obtained in studies from the capital of Norway. However, the same basic pattern of mental illness can be observed in the rural as in the urban area of Oslo, with alcohol abuse/dependence and major depression being the most common disorders at both sites. The sex pattern is also the same with higher figures for women both in rural and urban areas with the exception of alcohol and drug abuse being higher in men.
|
['Adolescent', 'Adult', 'Aged', 'Diagnostic and Statistical Manual of Mental Disorders', 'Female', 'Humans', 'Male', 'Mental Disorders', 'Mental Health Services', 'Middle Aged', 'Norway', 'Prevalence', 'Registries', 'Rural Population', 'Severity of Illness Index', 'Substance-Related Disorders']
| 16,732,397
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['L01.453.245.945.200'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03'], ['F04.408', 'N02.421.461'], ['M01.060.116.630'], ['Z01.542.816.374'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['N01.600.725'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['C25.775', 'F03.900']]
|
['Named Groups [M]', 'Information Science [L]', 'Organisms [B]', 'Psychiatry and Psychology [F]', 'Health Care [N]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
| 1
|
HMOs aggressively developing disease management programs; cost reductions reported.
|
A new report documents the percentage of HMOs experiencing cost reductions, by type of health services, after implementing disease management initiatives--and the numbers are encouraging.
|
['Asthma', 'Cost Savings', 'Diabetes Mellitus', 'Disease Management', 'Economics, Medical', 'Health Care Surveys', 'Health Maintenance Organizations', 'Humans', 'Specialization', 'United States']
| 10,174,905
|
[['C08.127.108', 'C08.381.495.108', 'C08.674.095', 'C20.543.480.680.095'], ['N03.219.151.160.200'], ['C18.452.394.750', 'C19.246'], ['N04.590.607'], ['N03.219.300'], ['E05.318.308.980.344', 'N03.349.380.210', 'N05.425.210', 'N05.715.360.300.800.344', 'N06.850.520.308.980.344'], ['N03.219.521.576.343.800.400', 'N03.219.521.576.343.925.400', 'N04.452.758.244.425', 'N04.590.374.410.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H02.811'], ['Z01.107.567.875']]
|
['Diseases [C]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
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| 1
|
CAISMOV24, a new human low-grade serous ovarian carcinoma cell line.
|
BACKGROUND: The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma.METHODS: The in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. The CAISMOV24 line was assessed for cell growth, production of soluble biomarkers, expression of surface molecules and screened for typical mutations found in serous ovarian carcinoma. Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells.RESULTS: CAISMOV24 has been in continuous culture for more than 30 months and more than 100 in vitro passages. The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. CAISMOV24 continues to produce CA125 and HE4 in vitro. Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. CAISMOV24 cell line harbored KRAS mutation with wild type TP53, therefore it is characterized as low-grade serous carcinoma.CONCLUSION: Our results corroborate with the idea that genomic alterations, depicted by CNVs, can be used for subtyping epithelial ovarian carcinomas. Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells.
|
['Biomarkers, Tumor', 'Cell Line, Tumor', 'Cell Proliferation', 'Cell Transformation, Neoplastic', 'Comparative Genomic Hybridization', 'Cystadenocarcinoma, Serous', 'Cytogenetic Analysis', 'Female', 'Humans', 'Immunohistochemistry', 'Immunophenotyping', 'Middle Aged', 'Mutation', 'Neoplasm Grading', 'Ovarian Neoplasms', 'Tumor Cells, Cultured']
| 29,132,324
|
[['D23.101.140'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['C04.697.098.500', 'C23.550.727.098.500'], ['E05.393.285.240', 'E05.393.520.500', 'E05.393.661.187'], ['C04.557.470.200.025.480.240', 'C04.557.470.590.480.240'], ['E01.370.225.500.385', 'E05.200.500.385', 'E05.242.385', 'E05.393.285'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['E01.370.225.812.447', 'E05.200.812.447', 'E05.478.594.450'], ['M01.060.116.630'], ['G05.365.590'], ['E01.789.612'], ['C04.588.322.455', 'C13.351.500.056.630.705', 'C13.351.937.418.685', 'C19.344.410', 'C19.391.630.705'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Named Groups [M]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Alcohol intoxication among adolescents and children in urban and rural environments - a retrospective analysis.
|
INTRODUCTION AND OBJECTIVE: Drinking alcohol by adolescents and children poses a risk of long-term psychological and sociological consequences, often leading to addiction in adulthood. A steady increase in the number of young people reaching for alcohol is worrying. The study analyzes the age and gender of the children, concentration of alcohol in the blood, depending on the origin of the youth (urban or rural).MATERIAL AND METHODS: The study was a retrospective analysis of 402 patients hospitalized due to alcohol intoxication in the Department of Paediatrics at Medical University in Lublin, Poland between 2004 - 2013.RESULTS: During the study period a continuous increase in admissions of patients after alcohol consumption was observed: from 27 children in 2004 to 53 in 2012 and 2013. The youngest patient hospitalized after drinking was 7.6 years old and came from the rural environment, the oldest 18 years old and came from the urban environment. In 2004 - 2007, boys dominated among children intoxicated with alcohol; since 2008, a slight prevalence of girls has been observed, especially in the urban environment. Among patients coming from the country, boys always predominated. In the study period there was noted a similar number of children consuming alcohol from rural and urban environments.CONCLUSIONS: The results suggest the need to introduce appropriate educational programmes in schools to prevent the consumption of alcohol at a young age.
|
['Adolescent', 'Adolescent Health', 'Alcoholic Intoxication', 'Child', 'Female', 'Hospitalization', 'Humans', 'Male', 'Poland', 'Prevalence', 'Retrospective Studies', 'Rural Population', 'Urban Population']
| 29,575,887
|
[['M01.060.057'], ['N01.400.075'], ['C25.775.100.175', 'F03.900.100.300'], ['M01.060.406'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.542.248.679'], ['E05.318.308.985.525.750', 'N01.224.935.597.750', 'N06.850.505.400.975.525.750', 'N06.850.520.308.985.525.750'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['N01.600.725'], ['N01.600.900']]
|
['Named Groups [M]', 'Health Care [N]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Characteristics of violent bars and bar patrons.
|
OBJECTIVE: The present analysis is an attempt to examine the characteristics of bars in which violence occurs while accounting for the personalities of the clientele that frequent the bar. It is proposed that an explanation of why violence occurs at certain bars requires examining the characteristics of the bars, the personalities of the clientele, and how these two types of variables act together in order to give rise to aggressive behavior.METHOD: We conducted interviews with frequent bar patrons (n = 327), assessing participants on a number of individual differences related to aggression and drinking behavior as well as on characteristics of the usual bar that they attend. Bars were categorized into violent bars (n = 256) or nonviolent bars (n = 71) based on participant responses.RESULTS: Participants' age, alcohol dependence and anger expression differentiated those who frequented violent bars from those who frequented nonviolent bars. The relationship of these individual differences to bar type was mediated by a number of characteristics of the bar itself, including noise, temperature, the presence of bouncers, the gender of the workers, the presence of billiards and illegal activities in the bar.CONCLUSIONS: The results indicate that individuals having certain personality characteristics are attracted to bar environments that promote antinormative behaviors such as violence. However, it seems to be the characteristics of the bars that are the strongest predictors of violence.
|
['Adult', 'Aggression', 'Alcohol Drinking', 'Chi-Square Distribution', 'Commerce', 'Female', 'Humans', 'Interviews as Topic', 'Logistic Models', 'Male', 'Odds Ratio', 'Personality Assessment', 'Social Environment', 'Violence']
| 14,743,938
|
[['M01.060.116'], ['F01.145.126.125', 'F01.145.813.045'], ['F01.145.317.269'], ['E05.318.740.994.300', 'G17.820.300', 'N05.715.360.750.750.200', 'N06.850.520.830.994.300'], ['J01.219'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.420', 'L01.399.250.520', 'N05.715.360.300.400', 'N06.850.520.308.420'], ['E05.318.740.500.525', 'E05.318.740.600.800.450', 'E05.318.740.750.450', 'E05.599.835.875', 'N05.715.360.750.530.480', 'N05.715.360.750.625.700.450', 'N05.715.360.750.695.470', 'N06.850.520.830.500.525', 'N06.850.520.830.600.800.450', 'N06.850.520.830.750.450'], ['E05.318.740.600.600', 'G17.680.500', 'N05.715.360.750.625.590', 'N06.850.520.830.600.600'], ['F04.513'], ['I01.880.853.500'], ['I01.198.240.856', 'I01.880.735.900']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Technology, Industry, and Agriculture [J]', 'Organisms [B]', 'Information Science [L]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 0
| 1
| 0
| 0
| 1
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
|
Molecular and functional characterizations of the association and interactions between nucleophosmin-anaplastic lymphoma kinase and type I insulin-like growth factor receptor.
|
Nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) is aberrantly expressed in a subset of T cell lymphoma that commonly affects children and young adults. NPM-ALK possesses significant oncogenic potential that was previously documented using in vitro and in vivo experimental models. The exact mechanisms by which NPM-ALK induces its effects are poorly understood. We have recently demonstrated that NPM-ALK is physically associated with type I insulin-like growth factor receptor (IGF-IR). A positive feedback loop appears to exist between NPM-ALK and IGF-IR through which these two kinases interact to potentiate their effects. We have also found that a single mutation of the Tyr(644) or Tyr(664) residue of the C terminus of NPM-ALK to phenylalanine decreases significantly, but does not completely abolish, the association between NPM-ALK and IGF-IR. The purpose of this study was to determine whether the dual mutation of Tyr(644) and Tyr(664) abrogates the association and interactions between NPM-ALK and IGF-IR. We also examined the impact of this dual mutation on the oncogenic potential of NPM-ALK. Our results show that NPM-ALK(Y644,664F) completely lacks association with IGF-IR. Importantly, we found that the dual mutation of Tyr(644) and Tyr(664) diminishes the oncogenic effects of NPM-ALK, including its ability to induce anchorage-independent colony formation and to sustain cellular transformation, proliferation, and migration. Furthermore, the association between NPM-ALK and IGF-IR through Tyr(644) and Tyr(664) appears to contribute to maintaining the stability of NPM-ALK protein. Our results provide novel insights into the mechanisms by which NPM-ALK induces its oncogenic effects through interactions with IGF-IR in this aggressive lymphoma.
|
['Animals', 'Benzamides', 'Cell Line, Tumor', 'Cell Movement', 'Cell Proliferation', 'Humans', 'Imatinib Mesylate', 'Lymphoma, T-Cell', 'Mice', 'Mutation', 'NIH 3T3 Cells', 'Phospholipase C gamma', 'Phosphorylation', 'Piperazines', 'Protein Stability', 'Protein-Tyrosine Kinases', 'Pyrimidines', 'Receptor, IGF Type 1', 'Receptor, Platelet-Derived Growth Factor beta', 'Tyrosine']
| 23,730,215
|
[['B01.050'], ['D02.065.277', 'D02.241.223.100.100', 'D02.455.426.559.389.127.085'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['G04.161.750', 'G07.345.249.410.750'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D02.065.277.456', 'D02.241.223.100.100.435', 'D02.455.426.559.389.127.085.465', 'D03.383.606.405', 'D03.383.742.349'], ['C04.557.386.480.750', 'C15.604.515.569.480.750', 'C20.683.515.761.480.750'], ['B01.050.150.900.649.313.992.635.505.500'], ['G05.365.590'], ['A11.251.210.100.550', 'A11.329.228.100.550'], ['D08.811.277.352.640.700.700.562.750', 'D12.644.360.571.750', 'D12.776.476.556.750'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D03.383.606'], ['G02.111.700'], ['D08.811.913.696.620.682.725'], ['D03.383.742'], ['D08.811.913.696.620.682.725.400.185', 'D12.776.543.750.630.468', 'D12.776.543.750.750.400.780.400'], ['D08.811.913.696.620.682.725.400.900.750', 'D12.776.543.750.630.625.400', 'D12.776.543.750.750.400.630.400'], ['D12.125.072.050.875']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Glucose intolerance is associated with C-reactive protein and intima-media anatomy of the common carotid artery in patients with coronary heart disease.
|
AIMS: The purpose of this study was to examine the relationship between glucose intolerance and levels of hsCRP, calculated intima-media area (cIMa) of the carotid artery and flow-mediated dilation of the brachial artery in 122 patients with a myocardial infarction 1-12 months before inclusion and without known diabetes mellitus.METHODS: A standard oral glucose test (OGTT) was performed. Diabetes mellitus and impaired glucose tolerance (IGT) were defined according to the WHO criteria. Ultrasound measurement of cIMa of the carotid artery and flow-mediated dilation of the brachial artery were analyzed.RESULTS: Patients with diabetes mellitus had higher hs-CRP compared with patients with IGT and those patients with normal glucose tolerance (P < 0.05). The greater cIMa of the carotid artery in those with diabetes mellitus compared with normal subjects failed to reach conventional levels of significance (P = 0.058). hs-CRP and cIMa were associated with plasma glucose 120 min after the glucose load (P < 0.05). A multiple stepwise regression analysis, including all variables significantly associated with plasma-glucose 120 min after glucose ingestion as independent variables, revealed an independent and significant association between plasma-glucose 120 min after glucose ingestion in the OGTT and CRP (P < 0.05). No association was observed between glucose intolerance and endothelial function.CONCLUSION: Glucose intolerance was associated with hs-CRP and cIMa in patients with coronary heart disease without known diabetes mellitus. Thus, inflammation, atherosclerosis and impaired glucose tolerance are tightly interrelated disorders even in subjects without known diabetes mellitus.
|
['Adult', 'Aged', 'Aged, 80 and over', 'Blood Glucose', 'Blood Pressure', 'Body Mass Index', 'Brachial Artery', 'C-Reactive Protein', 'Carotid Artery, Common', 'Diabetes Mellitus', 'Dilatation, Pathologic', 'Female', 'Glucose Intolerance', 'Glucose Tolerance Test', 'Heart Rate', 'Humans', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Triglycerides', 'Tunica Intima', 'Ultrasonography']
| 16,108,851
|
[['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['D09.947.875.359.448.500'], ['E01.370.600.875.249', 'G09.330.380.076'], ['E01.370.600.115.100.125', 'E05.041.124.125', 'G07.100.100.125', 'N06.850.505.200.100.175'], ['A07.015.114.139'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['A07.015.114.186.200'], ['C18.452.394.750', 'C19.246'], ['C23.300.325'], ['C18.452.394.952.500'], ['E01.370.225.124.100.355', 'E01.370.374.355', 'E05.200.124.100.355'], ['E01.370.600.875.500', 'G09.330.380.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['D10.351.801'], ['A07.015.700'], ['E01.370.350.850']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Health Care [N]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Changes in regional renal perfusion following ischemia/reperfusion injury to the rat kidney.
|
Post-ischemic renal failure is associated with a zone of vascular hyperaemia in the outer medulla of the kidney. The effect of this lesion on regional renal perfusion is, however, unclear. Acute unilateral renal ischemia was applied to four groups of ten adult male Wistar rats for a period of 60 min, followed by revascularisation for 0, 15, 30 or 60 min. The aorta was then clamped and Microfil was injected at a standard pressure to fill the renal vasculature. Gross and histological examinations of the renal parenchyma and vasculature were then performed. Regional renal Microfil perfusion was quantified by examination of unstained histological sections, giving rise to a vascular perfusion index (VPI) for each vascular region of the kidney. The VPIs were similar in control and ischemic kidneys that were not subjected to reflow (group 1). In contrast, the VPI was markedly decreased in the inner stripe and inner medulla in animals in which revascularisation had occurred (groups 2-4), and the vasculature in these regions was histologically shown to be packed with red blood cells. Post-ischemic renal failure is associated with hyperperfusion of the medulla resulting from blockage of the vasculature that occurs during revascularisation.
|
['Acute Kidney Injury', 'Animals', 'Kidney', 'Male', 'Rats', 'Rats, Inbred Strains', 'Renal Circulation', 'Reperfusion Injury']
| 1,926,662
|
[['C12.777.419.780.050', 'C13.351.968.419.780.050'], ['B01.050'], ['A05.810.453'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['G08.852.725', 'G09.330.100.812'], ['C14.907.725', 'C23.550.767.877']]
|
['Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Role of impact velocity and chest compression in thoracic injury.
|
Impact velocity and chest compression are important factors in traumatic injury; however, there is no functional relationship to assess impact severity. A blunt thoracic impact of constant velocity (5-22 m/s) and prescribed contact displacement (3-46 mm) was delivered to 123 anesthetized rabbits. Myocardial and major vascular injury increased from contusion to rupture with cardiac tamponade and sudden death as either impact velocity or chest compression was independently increased. A theoretical relationship was found for impact severity: IS=VC/1-C, where V and C are impact velocity and normalized chest compression. Based on the frequency of critical/fatal injury, probit analysis gave IS=6.4 m/s as an estimate of the ED50 response in the experimental model.
|
['Animals', 'Cardiac Tamponade', 'Cardiovascular System', 'Death, Sudden', 'Heart Injuries', 'Male', 'Rabbits', 'Rupture', 'Thoracic Injuries']
| 6,830,552
|
[['B01.050'], ['C14.280.155'], ['A07'], ['C23.550.260.322'], ['C26.891.375'], ['B01.050.150.900.649.313.968.700'], ['C26.761'], ['C26.891']]
|
['Organisms [B]', 'Diseases [C]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Comparison of predictive control methods for high consumption industrial furnace.
|
We describe several predictive control approaches for high consumption industrial furnace control. These furnaces are major consumers in production industries, and reducing their fuel consumption and optimizing the quality of the products is one of the most important engineer tasks. In order to demonstrate the benefits from implementation of the advanced predictive control algorithms, we have compared several major criteria for furnace control. On the basis of the analysis, some important conclusions have been drawn.
|
['Air Pollutants', 'Algorithms', 'Predictive Value of Tests', 'Soot']
| 24,319,354
|
[['D27.888.284.101'], ['G17.035', 'L01.224.050'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['D20.633.937.339']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
Comparative mechanical analysis of a looped-suture tendon repair.
|
The in vitro breaking force of a braided nylon looped-suture tendon juncture designed to decrease tying time was compared with the breaking force of the modified Kessler and Bunnell techniques. Repaired with either braided nylon or tetrafluoroethylene, porcine digiti quarti propius tendons were tested to single cycle failure on a MTS hydraulic testing machine. The results showed that the looped-suture technique had a mean breaking force that was statistically indistinguishable from that of the Bunnell technique regardless of suture material. However, the breaking forces for the looped suture and Bunnell techniques were statistically greater for both suture materials when compared with the modified Kessler technique. The resistance to gap formation for the looped suture was found to be intermediate between the Bunnell technique and the modified Kessler technique.
|
['Animals', 'Biomechanical Phenomena', 'Fluorocarbons', 'Nylons', 'Polyesters', 'Suture Techniques', 'Swine', 'Tendons', 'Tensile Strength']
| 3,071,546
|
[['B01.050'], ['G01.154.090', 'G01.374.089'], ['D02.455.526.510.435'], ['D05.750.716.392', 'D25.720.716.392', 'J01.637.051.720.716.392', 'J01.637.548'], ['D05.750.728', 'D25.720.728', 'J01.637.051.720.728'], ['E04.987.775'], ['B01.050.150.900.649.313.500.880'], ['A02.880'], ['G01.374.850']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Schizosaccharomyces pombe Grx4 regulates the transcriptional repressor Php4 via [2Fe-2S] cluster binding.
|
The fission yeast Schizosaccharomyces pombe expresses the CCAAT-binding factor Php4 in response to iron deprivation. Php4 forms a transcription complex with Php2, Php3, and Php5 to repress the expression of iron proteins as a means to economize iron usage. Previous in vivo results demonstrate that the function and location of Php4 are regulated in an iron-dependent manner by the cytosolic CGFS type glutaredoxin Grx4. In this study, we aimed to biochemically define these protein-protein and protein-metal interactions. Grx4 was found to bind a [2Fe-2S] cluster with spectroscopic features similar to other CGFS glutaredoxins. Grx4 and Php4 also copurify as a complex with a [2Fe-2S] cluster that is spectroscopically distinct from the cluster on Grx4 alone. In vitro titration experiments suggest that these Fe-S complexes may not be interconvertible in the absence of additional factors. Furthermore, conserved cysteines in Grx4 (Cys172) and Php4 (Cys221 and Cys227) are necessary for Fe-S cluster binding and stable complex formation. Together, these results show that Grx4 controls Php4 function through binding of a bridging [2Fe-2S] cluster.
|
['CCAAT-Binding Factor', 'Cysteine', 'Gene Expression Regulation, Fungal', 'Glutaredoxins', 'Iron', 'Iron-Sulfur Proteins', 'Models, Molecular', 'Schizosaccharomyces', 'Schizosaccharomyces pombe Proteins', 'Signal Transduction']
| 28,725,905
|
[['D12.776.260.108.124.249', 'D12.776.660.167.249', 'D12.776.930.127.124.249'], ['D02.886.030.230', 'D02.886.489.155', 'D12.125.154.299', 'D12.125.166.230'], ['G05.308.330'], ['D08.811.682.667.084'], ['D01.268.556.412', 'D01.268.956.287', 'D01.552.544.412'], ['D12.776.157.427.374.375', 'D12.776.556.579.374.375'], ['E05.599.595'], ['B01.300.107.797', 'B01.300.930.720'], ['D12.776.354.875'], ['G02.111.820', 'G04.835']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Germectomy. The germ of the third mandibular molar in children: remove or preserve?].
|
Third molars are often removed in order to prevent complications and various other problems associated with impacted third molars and their removal. Abortion of mandibular third molars is a procedure carried out at an early age in those subjects where there is insufficient room for the eruption of the third molars. On the other hand one can also decide to remove the second molars and to annexate orthodontically the third molars in the arch.
|
['Child', 'Humans', 'Mandible', 'Molar, Third', 'Tooth Erosion', 'Tooth Germ', 'Tooth, Impacted']
| 11,820,009
|
[['M01.060.406'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['A14.549.167.860.525.500'], ['C07.793.818.500'], ['A14.549.167.900.720'], ['C07.793.905']]
|
['Named Groups [M]', 'Organisms [B]', 'Anatomy [A]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Neuropeptide F regulates courtship in Drosophila
|
Male courtship is provoked by perception of a potential mate. In addition, the likelihood and intensity of courtship are influenced by recent mating experience, which affects sexual drive. Using Drosophila melanogaster, we found that the homolog of mammalian neuropeptide Y, neuropeptide F (NPF), and a cluster of male-specific NPF (NPFM) neurons, regulate courtship through affecting courtship drive. Disrupting NPF signaling produces sexually hyperactive males, which are resistant to sexual satiation, and whose courtship is triggered by sub-optimal stimuli. We found that NPFM neurons make synaptic connections with P1 neurons, which comprise the courtship decision center. Activation of P1 neurons elevates NPFM neuronal activity, which then act through NPF receptor neurons to suppress male courtship, and maintain the proper level of male courtship drive.
|
['Animals', 'Courtship', 'Drosophila Proteins', 'Drosophila melanogaster', 'Male', 'Neural Pathways', 'Neurons', 'Neuropeptides']
| 31,403,399
|
[['B01.050'], ['F01.145.802.279'], ['D12.776.093.500.462'], ['B01.050.500.131.617.720.500.500.750.310.250.500'], ['A08.612'], ['A08.675', 'A11.671'], ['D12.644.400', 'D12.776.631.650']]
|
['Organisms [B]', 'Psychiatry and Psychology [F]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A spectroscopic and thermal investigation into the relationship between composition, secondary structure and physical characteristics of electrospun zein nanofibers.
|
Electrospun zein nanofibers have attracted interest as drug delivery systems due to their propensity for controlled drug release, flexible structure and low toxicity. However, comparatively little is known regarding the relationship between production method and fiber characteristics, both in terms of fiber architecture and protein structure. Here we use a range of imaging and spectroscopic techniques to elucidate the effects of solvent composition on zein secondary structure, fiber diameter and fiber integrity, plus we utilize the new technique of transition temperature microscopy to examine the thermal properties of the fibers. Zein nanofibers were prepared using ethanol, acetic acid and water mixes as solvents, alone and with plasticizers (polyethylene glycol, glycerol) and casein. Electrospinning was performed under controlled conditions and the products characterized using scanning electron microscopy (SEM), attenuated total reflection Fourier Transform infrared spectrometry (ATR - FTIR) and transition temperature microscopy (TTM). The choice of solvent, concentration and voltage, alongside the presence of additives (plasticizers and casein) were noted to influence both the diameter of the fibers and the tendency for bead formation. A relationship was noted between protein secondary structure and fiber architecture, with an enhanced â-sheet content, enhanced by the inclusion of casein, being associated with higher beading. In addition, thermal imaging of electrospun zein fiber mats was successfully achieved using TTM via two dimensional mapping of the softening temperatures across the spun samples, in particular demonstrating the plasticizing effects of the polyethylene glycol and glycerol.
|
['Electric Conductivity', 'Microscopy', 'Nanofibers', 'Nanotechnology', 'Protein Structure, Secondary', 'Solutions', 'Solvents', 'Spectroscopy, Fourier Transform Infrared', 'Temperature', 'Transition Temperature', 'Viscosity', 'Zein']
| 30,813,042
|
[['G01.358.500.249.277'], ['E01.370.350.515', 'E05.595', 'H01.671.617.562'], ['J01.637.512.300'], ['H01.603', 'J01.897.520.600'], ['G02.111.570.820.709.600'], ['D26.776'], ['D27.720.844'], ['E05.196.712.726.676.700', 'E05.196.867.826.676.700'], ['G01.906.595', 'G16.500.275.063.725.710', 'G16.500.750.775.710', 'N06.230.150.450', 'N06.230.300.100.725.710'], ['G01.906.595.850'], ['G02.930'], ['D12.776.765.433.500.750', 'D12.776.765.725.500.750']]
|
['Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
Automatic snoring detection from nasal pressure data.
|
This study presents a method for automatic snoring detection from a nasal pressure data. First, a spectrogram analysis was performed in order to obtain information about the spectral characteristic of nasal pressure data. The automatic method is based on a simple signal filtering and short-time energy technique. Fifteen patients were participated in order to evaluation the performance of the proposed method. Results are compared with manually labeled snoring events by watching video records. The sensitivity and positive predictivity value were 93.73% and 93.70%, respectively. The results in this study could provide sleep experts with the method to objectively monitor sleep-disordered breathing in CPAP system or PSG study.
|
['Adult', 'Female', 'Humans', 'Male', 'Middle Aged', 'Nose', 'Polysomnography', 'Predictive Value of Tests', 'Pressure', 'Signal Processing, Computer-Assisted', 'Snoring', 'Spectrum Analysis', 'Young Adult']
| 24,111,323
|
[['M01.060.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['A01.456.505.733', 'A04.531', 'A09.531'], ['E01.370.520.625'], ['E05.318.370.800.650', 'N05.715.360.325.700.640', 'N06.850.520.445.800.650'], ['G01.374.715'], ['L01.224.800'], ['C23.888.852.779.850'], ['E05.196.867'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Mechanism of the dehydrogenase reaction of DmpFG and analysis of inter-subunit channeling efficiency and thermodynamic parameters in the overall reaction.
|
The bifunctional, microbial enzyme DmpFG is comprised of two subunits: the aldolase, DmpG, and the dehydrogenase, DmpF. DmpFG is of interest due to its ability to channel substrates between the two spatially distinct active sites. While the aldolase is well studied, significantly less is known about the dehydrogenase. Steady-state kinetic measurements of the reverse reaction of DmpF confirmed that the dehydrogenase uses a ping-pong mechanism, with substrate inhibition by acetyl CoA indicating that NAD(+)/NADH and CoA/acetyl CoA bind to the same site in DmpF. The Km of DmpF for exogenous acetaldehyde as a substrate was 23.7 mM, demonstrating the necessity for the channel to deliver acetaldehyde directly from the aldolase to the dehydrogenase active site. A channeling assay on the bifunctional enzyme gave an efficiency of 93% indicating that less than 10% of the toxic acetaldehyde leaks out of the channel into the bulk media, prior to reaching the dehydrogenase active site. The thermodynamic activation parameters of the reactions catalyzed by the aldolase, the dehydrogenase and the DmpFG complex were determined. The Gibb's free energy of activation for the dehydrogenase reaction was lower than that obtained for the full DmpFG reaction, in agreement with the high kcat obtained for the dehydrogenase reaction in isolation. Furthermore, although both the DmpF and DmpG reactions occur with small, favorable entropies of activation, the full DmpFG reaction occurs with a negative entropy of activation. This supports the concept of allosteric structural communication between the two enzymes to coordinate their activities.
|
['Allosteric Regulation', 'Biocatalysis', 'Enzyme Activation', 'Fructose-Bisphosphate Aldolase', 'Keto Acids', 'Kinetics', 'Oxidoreductases', 'Protein Subunits', 'Pseudomonas', 'Substrate Specificity', 'Thermodynamics']
| 23,742,989
|
[['G02.111.044'], ['G02.111.086', 'G02.130.500', 'G03.105'], ['G02.111.263', 'G03.328'], ['D08.811.520.224.062.400'], ['D02.241.755'], ['G01.374.661', 'G02.111.490'], ['D08.811.682'], ['D12.776.813'], ['B03.440.400.425.625.625', 'B03.660.250.580.590'], ['G02.111.835'], ['G01.906']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Determination of prolactin, growth hormone, beta-endorphin, and cortisol in both maternal plasma and amniotic fluid during human gestation.
|
This study focuses on PRL, GH, beta-endorphin and cortisol in maternal blood and amniotic fluid during human pregnancy. Maternal blood and amniotic fluid samples were obtained from 18 normal pregnant women in the second trimester, 12 full-term gravidas having spontaneous delivery, and 10 full-term gravidas having elective cesarean section. Two gravidas bearing anencephalic fetuses in the third trimester were also studied. In the second trimester women, levels of PRL (3215.9 +/- 458.9 micrograms/l), GH (19.1 +/- 1.7 micrograms/l) and beta-endorphin (11.1 +/- 0.9 pmol/l) were significantly higher in the amniotic fluid than in maternal plasma. In addition, PRL was significantly correlated with beta-endorphin (r = 0.670) and with GH (r = 0.547) in the amniotic fluid. However, amniotic fluid cortisol levels (0.27 +/- 0.18 nmol/l) were significantly lower than plasma cortisol levels. The amniotic fluid of the women with anencephalic fetuses had normal levels of PRL, GH and beta-endorphin. In full-term gravidas, plasma PRL levels were significantly lower in women with vaginal delivery than in those with elective cesarean section, and there was a significant negative correlation between plasma PRL and beta-endorphin, and between plasma PRL and cortisol levels. Plasma GH levels in women with vaginal delivery showed no significant difference from those in women with cesarean section. Examination of amniotic fluid yielded no significant differences in the levels of PRL, beta-endorphin and GH between these two groups.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Adult', 'Amniotic Fluid', 'Female', 'Growth Hormone', 'Humans', 'Hydrocortisone', 'Labor, Obstetric', 'Pregnancy', 'Prolactin', 'beta-Endorphin']
| 2,528,262
|
[['M01.060.116'], ['A12.098', 'A16.378.149'], ['D06.472.699.631.525.425', 'D12.644.548.691.525.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D04.210.500.745.745.654.600', 'D06.472.040.585.353.476', 'D06.472.040.585.478.392'], ['G08.686.784.769.326'], ['G08.686.784.769'], ['D06.472.699.322.576.773', 'D06.472.699.631.525.525', 'D12.644.548.691.525.525'], ['D06.472.699.327.935.239', 'D06.472.699.631.525.600.239', 'D12.644.400.400.935.239', 'D12.644.400.575.241.080', 'D12.644.548.365.935.239', 'D12.644.548.691.525.690.239', 'D12.776.631.650.405.935.239', 'D12.776.631.650.575.241.080']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Population branching in the conical intersection of the retinal chromophore revealed by multipulse ultrafast optical spectroscopy.
|
The branching ratio of the excited-state population at the conical intersection between the S(1) and S(0) energy surfaces (Ö(CI)) of a protonated Schiff base of all-trans retinal in protic and aprotic solvents was studied by multipulse ultrafast transient absorption spectroscopy. In particular, pump-dump-probe experiments allowed to isolate the S(1) reactive state and to measure the photoisomerization time constant with unprecedented precision. Starting from these results, we demonstrate that the polarity of the solvent is the key factor influencing the Ö(CI) and the photoisomerization yield.
|
['Bacteriorhodopsins', 'Electrochemistry', 'Retinaldehyde', 'Rhodopsin', 'Spectrum Analysis']
| 22,145,869
|
[['D12.776.090.200', 'D12.776.157.530.450.250.875.249', 'D12.776.543.585.450.250.875.249', 'D12.776.752.812.249'], ['H01.181.529.307'], ['D02.047.850', 'D02.455.326.271.665.202.495.690', 'D02.455.426.392.368.367.379.249.700.690', 'D02.455.849.131.495.690', 'D02.455.849.291.605', 'D23.767.261.700.690'], ['D12.776.543.750.695.955', 'D23.767.930.750.500.500'], ['E05.196.867']]
|
['Chemicals and Drugs [D]', 'Disciplines and Occupations [H]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
The protective effect of hypercapnia on ischemia-reperfusion injury in lungs.
|
Lifesaving therapy for patients with end-stage lung disease is lung transplantation. However, there are not enough available donors. A relatively new method of transplantation from non-heart-beating donors (NHBDs) allows the treatment of the lung outside the body and could increase the number of suitable lungs. We have focused on hypercapnic ventilation, which has the possibility of reducing reactive oxygen species damage. We used four experimental and two control groups of adult rats. Each experimental group underwent the protocol of NHBD lung harvesting. The lungs were than perfused in an ex vivo model and we measured weight gain, arterial-venous difference in partial pressure of oxygen and perfusion pressure. We observed that hypercapnic ventilation during reperfusion reduces the development of pulmonary oedema and has a protective effect on the oxygen transport ability of the lungs after warm ischemia. The effect of CO2 on pulmonary oedema and on oxygen transport ability after warm ischemia could be of clinical importance for NHBD transplantation.
|
['Animals', 'Disease Models, Animal', 'Hypercapnia', 'Lung Transplantation', 'Male', 'Rats', 'Rats, Wistar', 'Reperfusion Injury']
| 25,450,116
|
[['B01.050'], ['C22.232', 'E05.598.500', 'E05.599.395.080'], ['C23.888.852.544'], ['E04.928.600.495', 'E04.936.450.495'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['C14.907.725', 'C23.550.767.877']]
|
['Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Mutations in ribonucleic acid binding protein gene cause familial dilated cardiomyopathy.
|
OBJECTIVES: We sought to identify a novel gene for dilated cardiomyopathy (DCM).BACKGROUND: DCM is a heritable, genetically heterogeneous disorder that remains idiopathic in the majority of patients. Familial cases provide an opportunity to discover unsuspected molecular bases of DCM, enabling pre-clinical risk detection.METHODS: Two large families with autosomal-dominant DCM were studied. Genome-wide linkage analysis was used to identify a disease locus, followed by fine mapping and positional candidate gene sequencing. Mutation scanning was then performed in 278 unrelated subjects with idiopathic DCM, prospectively identified at the Mayo Clinic.RESULTS: Overlapping loci for DCM were independently mapped to chromosome 10q25-q26. Deoxyribonucleic acid sequencing of affected individuals in each family revealed distinct heterozygous missense mutations in exon 9 of RBM20, encoding ribonucleic acid (RNA) binding motif protein 20. Comprehensive coding sequence analyses identified missense mutations clustered within this same exon in 6 additional DCM families. Mutations segregated with DCM (peak composite logarithm of the odds score >11.49), were absent in 480 control samples, and altered residues within a highly conserved arginine/serine (RS)-rich region. Expression of RBM20 messenger RNA was confirmed in human heart tissue.CONCLUSIONS: Our findings establish RBM20 as a DCM gene and reveal a mutation hotspot in the RS domain. RBM20 is preferentially expressed in the heart and encodes motifs prototypical of spliceosome proteins that regulate alternative pre-messenger RNA splicing, thus implicating a functionally distinct gene in human cardiomyopathy. RBM20 mutations are associated with young age at diagnosis, end-stage heart failure, and high mortality.
|
['Adolescent', 'Adult', 'Aged', 'Cardiomyopathy, Dilated', 'Child, Preschool', 'Female', 'Genetic Linkage', 'Genome-Wide Association Study', 'Genotype', 'Heterozygote', 'Humans', 'Lod Score', 'Male', 'Middle Aged', 'Mutation, Missense', 'Myocardium', 'Pedigree', 'Phenotype', 'RNA, Messenger', 'RNA-Binding Proteins', 'Spliceosomes', 'Young Adult']
| 19,712,804
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['C14.280.195.160', 'C14.280.238.070', 'C16.320.488.750'], ['M01.060.406.448'], ['G05.348'], ['E05.318.370.392', 'E05.318.416.249', 'E05.393.385.500', 'E05.393.522.500', 'E05.393.760.640.500', 'N06.850.520.445.392', 'N06.850.520.470.500'], ['G05.380'], ['G05.380.383'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.348.750'], ['M01.060.116.630'], ['G05.365.590.650'], ['A02.633.580', 'A07.541.704', 'A10.690.552.750'], ['E05.393.673'], ['G05.695'], ['D13.444.735.544'], ['D12.776.157.725', 'D12.776.664.962'], ['A11.284.430.106.279.345.850'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Nek7 Protects Telomeres from Oxidative DNA Damage by Phosphorylation and Stabilization of TRF1.
|
Telomeric repeat binding factor 1 (TRF1) is essential to the maintenance of telomere chromatin structure and integrity. However, how telomere integrity is maintained, especially in response to damage, remains poorly understood. Here, we identify Nek7, a member of the Never in Mitosis Gene A (NIMA) kinase family, as a regulator of telomere integrity. Nek7 is recruited to telomeres and stabilizes TRF1 at telomeres after damage in an ATM activation-dependent manner. Nek7 deficiency leads to telomere aberrations, long-lasting ãH2AX and 53BP1 foci, and augmented cell death upon oxidative telomeric DNA damage. Mechanistically, Nek7 interacts with and phosphorylates TRF1 on Ser114, which prevents TRF1 from binding to Fbx4, an Skp1-Cul1-F box E3 ligase subunit, thereby alleviating proteasomal degradation of TRF1, leading to a stable association of TRF1 with Tin2 to form a shelterin complex. Our data reveal a mechanism of efficient protection of telomeres from damage through Nek7-dependent stabilization of TRF1.
|
['Ataxia Telangiectasia Mutated Proteins', 'Binding Sites', 'DNA Damage', 'F-Box Proteins', 'HEK293 Cells', 'HeLa Cells', 'Histones', 'Humans', 'NIMA-Related Kinases', 'Oxidative Stress', 'Phosphorylation', 'Proteasome Endopeptidase Complex', 'Protein Binding', 'Protein Stability', 'RNA Interference', 'Telomere', 'Telomere-Binding Proteins', 'Time Factors', 'Transfection', 'Tumor Suppressor p53-Binding Protein 1', 'Ubiquitination']
| 28,216,227
|
[['D08.811.913.696.620.682.700.097', 'D12.776.157.687.125', 'D12.776.660.720.125'], ['G02.111.570.120'], ['G05.200'], ['D12.776.157.169'], ['A11.251.210.172.750', 'A11.436.334'], ['A11.251.210.190.400', 'A11.251.860.180.400', 'A11.436.340'], ['D12.776.157.687.485', 'D12.776.660.720.485', 'D12.776.664.469'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D08.811.913.696.620.682.700.581', 'D12.776.167.457'], ['G03.673', 'G07.775.750'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['D05.500.562.500', 'D08.811.277.656.918', 'D08.811.600.730'], ['G02.111.679', 'G03.808'], ['G02.111.700'], ['G05.308.203.374.790'], ['A11.284.430.106.279.345.190.160.845', 'G05.360.160.845'], ['D12.776.260.735', 'D12.776.660.235.700'], ['G01.910.857'], ['E05.393.350.810', 'G05.728.860'], ['D12.776.260.805', 'D12.776.660.235.850', 'D12.776.664.235.950'], ['G02.111.660.871.790.600.925', 'G02.111.691.600.775', 'G03.734.871.790.600.831', 'G05.308.670.600.831']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Anterior dislocation of the ulnar-humeral joint in a so-called 'pulled elbow'.
|
We describe an unusual case of a missed anterior dislocation of the elbow joint in a 1 year old girl who presented with a pulled elbow. To our knowledge, this is the first report of anterior dislocation as a result of a pulled elbow in the literature. We would like to highlight the rarity of this presentation and the importance of chronological assessment and management in the accident and emergency department.
|
['Elbow Joint', 'Female', 'Humans', 'Immobilization', 'Infant', 'Joint Dislocations', 'Radiography', 'Radius', 'Treatment Outcome', 'Ulna']
| 16,714,491
|
[['A02.835.583.290'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.472'], ['M01.060.703'], ['C05.550.518', 'C26.289'], ['E01.370.350.700'], ['A02.835.232.087.090.700'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800'], ['A02.835.232.087.090.850']]
|
['Anatomy [A]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Health Care [N]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Risk factors for mortality in lower intestinal bleeding.
|
BACKGROUND & AIMS: Previous studies of lower intestinal bleeding (LIB) have limited power to study mortality. We sought to identify characteristics associated with in-hospital mortality in a large cohort of patients with LIB.METHODS: We used the 2002 Healthcare Cost and Utilization Project Nationwide Inpatient Sample to study a cross-sectional cohort of 227,022 hospitalized patients with discharge diagnoses indicating LIB. Predictors of mortality were identified by using multiple logistic regression.RESULTS: In 2002, an estimated 8737 patients with LIB (3.9%) died while hospitalized. Independent predictors of in-hospital mortality were age (age >70 vs <50 years; odds ratio [OR], 4.91; 95% confidence interval [CI], 2.45-9.87), intestinal ischemia (OR, 3.47; 95% CI, 2.57-4.68), comorbid illness (>or=2 vs 0 comorbidities, OR, 3.00; 95% CI, 2.25-3.98), bleeding while hospitalized for a separate process (OR, 2.35; 95% CI, 1.81-3.04), coagulation defects (OR, 2.34; 95% CI, 1.50-3.65), hypovolemia (OR, 2.22; 95% CI, 1.69-2.90), transfusion of packed red blood cells (OR, 1.60; 95% CI, 1.23-2.08), and male gender (OR, 1.52; 95% CI, 1.21-1.92). Colorectal polyps (OR, 0.26; 95% CI, 0.15-0.45), and hemorrhoids (OR, 0.42; 95% CI, 0.28-0.64) were associated with a lower risk of mortality, as was diagnostic testing for LIB when added to the multivariate model (OR, 0.37; 95% CI, 0.28-0.48). Hospital characteristics were not significantly related to mortality. Predictors of mortality were similar in an analysis restricted to patients with diverticular bleeding.CONCLUSIONS: The all-cause in-hospital mortality rate in LIB was low (3.9%). Advanced age, intestinal ischemia, and comorbid illness were the strongest predictors of mortality.
|
['Age Factors', 'Aged', 'Aged, 80 and over', 'Blood Coagulation Disorders', 'Cohort Studies', 'Colonic Diseases', 'Comorbidity', 'Cross-Sectional Studies', 'Female', 'Gastrointestinal Hemorrhage', 'Hospitalization', 'Humans', 'Hypovolemia', 'Ischemia', 'Male', 'Middle Aged', 'Risk Factors', 'Sex Factors', 'Transfusion Reaction']
| 18,558,513
|
[['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C15.378.100'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['C06.405.469.158'], ['N05.715.350.225', 'N06.850.490.687'], ['E05.318.372.500.875', 'N05.715.360.330.500.875', 'N06.850.520.450.500.875'], ['C06.405.227', 'C23.550.414.788'], ['E02.760.400', 'N02.421.585.400'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C23.550.455'], ['C23.550.513'], ['M01.060.116.630'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['N05.715.350.675', 'N06.850.490.875'], ['C15.378.962', 'C20.920']]
|
['Health Care [N]', 'Named Groups [M]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Genetic evidence that high noninduced maltase and maltose permease activities, governed by MALx3-encoded transcriptional regulators, determine efficiency of gas production by baker's yeast in unsugared dough.
|
Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of maltose utilization. One group (nonlagging strains), characterized by rapid maltose fermentation, had at least 12-fold more maltase and 130-fold-higher maltose permease activities than maltose-lagging strains in the absence of inducing sugar (maltose) and repressing sugar (glucose). Increasing the noninduced maltase activity of a lagging strain 13-fold led to an increase in CO2 production in unsugared dough. This increase in CO2 production also was seen when the maltose permease activity was increased 55-fold. Only when maltase and maltose permease activities were increased in concert was CO2 production by a lagging strain similar to that of a nonlagging strain. The noninduced activities of maltase and maltose permease constitute the largest determinant of whether a strain displays a nonlagging or a lagging phenotype and are dependent upon the MALx3 allele. Previous strategies for strain improvement have targeted glucose derepression of maltase and maltose permease expression. Our results suggest that increasing noninduced maltase and maltose permease levels is an important target for improved maltose metabolism in unsugared dough.
|
['Carbon Dioxide', 'Enzyme Induction', 'Fermentation', 'Food Microbiology', 'Genes, Fungal', 'Glucose', 'Maltose', 'Membrane Transport Proteins', 'Monosaccharide Transport Proteins', 'Saccharomyces cerevisiae', 'Transcription Factors', 'alpha-Glucosidases']
| 9,925,600
|
[['D01.200.200', 'D01.362.150', 'D01.650.550.200'], ['G05.308.320.200'], ['G02.111.158.249', 'G03.191.249'], ['H01.158.273.540.274.332', 'J01.576.423.850.730.500.249.300', 'N06.850.425.200', 'N06.850.460.400.300', 'N06.850.601.500.249.300'], ['G05.360.340.024.340.364.500', 'G05.360.340.358.024.500', 'G05.360.340.358.365.500'], ['D09.947.875.359.448'], ['D09.698.365.450', 'D09.698.629.305.523', 'D09.947.750.523'], ['D12.776.157.530', 'D12.776.543.585'], ['D12.776.157.530.500', 'D12.776.543.585.500'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['D12.776.930'], ['D08.811.277.450.420.050']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
|
What's wrong with me? Women's coronary heart disease diagnostic experiences.
|
Most women are unaware that that they may experience atypical coronary heart disease (CHD) symptoms. Women's atypical presentation often results in women having difficulty being diagnosed with CHD or myocardial infarction. Investigating women's CHD diagnostic experiences may reveal vital areas amenable to intervention. This secondary analysis explored women's CHD diagnostic experiences. Forty women completed in-depth interviews in their homes that were audiotaped and lasted 2-3 hours. Using content analysis and constant comparison, five themes emerged: awareness, seeking treatment, frustration, treatment decisions, and anger. Despite numerous symptoms and visits with clinicians, most women were not diagnosed with CHD before myocardial infarction. During the infarction, women with typical symptoms were easily diagnosed while those with atypical symptoms received a delayed diagnosis. Those who repeatedly sought treatment were angry about not being diagnosed earlier. Further research is needed to promote early symptom recognition, timely diagnosis, and efficacious treatment-keys to improving women's CHD outcomes and to preventing similar negative diagnostic experiences.
|
['Adaptation, Psychological', 'Adult', 'Aged', 'Anger', 'Anthropology, Cultural', 'Arkansas', 'Attitude of Health Personnel', 'Awareness', 'Chest Pain', 'Decision Making', 'Denial, Psychological', 'Female', 'Frustration', 'Health Knowledge, Attitudes, Practice', 'Heartburn', 'Humans', 'Middle Aged', 'Models, Psychological', 'Myocardial Infarction', 'Nursing Methodology Research', 'Patient Acceptance of Health Care', 'Professional-Patient Relations', 'Qualitative Research', 'Retrospective Studies', 'Surveys and Questionnaires', 'Time Factors', 'Women']
| 15,886,547
|
[['F01.058'], ['M01.060.116'], ['M01.060.116.100'], ['F01.470.093'], ['I01.076.201'], ['Z01.107.567.875.750.110'], ['F01.100.050', 'N05.300.100'], ['F02.463.188.150'], ['C23.888.592.612.233'], ['F02.463.785.373'], ['F01.393.200'], ['F01.470.405'], ['F01.100.150.500', 'N05.300.150.410'], ['C23.888.821.525'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E05.599.695'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['H01.770.644.145.390.634', 'H02.478.395.634', 'N04.590.233.508.613.634'], ['F01.100.150.750.500', 'F01.145.488.887.500', 'N05.300.150.800.500'], ['F01.829.401.650', 'N05.300.660'], ['H01.770.644.241.850'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980'], ['G01.910.857'], ['M01.975']]
|
['Psychiatry and Psychology [F]', 'Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Geographicals [Z]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 1
| 1
|
CD146 attenuation in cancer-associated fibroblasts promotes pancreatic cancer progression.
|
Cancer-associated fibroblasts (CAFs) are heterogeneous cell populations that influence tumor initiation and progression. CD146 is a cell membrane protein whose expression has been implicated in multiple human cancers. CD146 expression is also detected in pancreatic cancer stroma; however, the role it plays in this context remains unclear. This study aimed to clarify the function and significance of CD146 expression in pancreatic cancer. We performed immunohistochemical staining to investigate the prevalence of CD146 expression in stromal fibroblasts in pancreatic cancer. We also examined the influence of CD146 on CAF-mediated tumor invasion and migration and CAF activation using CD146 small interfering RNA or overexpression plasmids in primary cultures of CAFs derived from pancreatic cancer tissues. CD146 expression in CAFs was associated with high-grade pancreatic intraepithelial neoplasia and low histological grade invasive ductal carcinoma of the pancreas, while patients with low CD146 expression had a poorer prognosis. Blocking CD146 expression in CAFs significantly enhanced tumor cell migration and invasion in a co-culture system. CD146 knockdown also promoted CAF activation, possibly by inducing the production of pro-tumorigenic factors through modulation of NF-êB activity. Consistently, overexpression of CD146 in CAFs inhibited migration and invasion of co-cultured cancer cells. Finally, CD146 expression in CAFs was reduced by interaction with cancer cells. Our findings suggest that decreased CD146 expression in CAFs promotes pancreatic cancer progression. © 2015 Wiley Periodicals, Inc.
|
['CD146 Antigen', 'Cancer-Associated Fibroblasts', 'Cell Line, Tumor', 'Cell Movement', 'Cell Proliferation', 'Coculture Techniques', 'Disease Progression', 'Down-Regulation', 'Female', 'Gene Expression Regulation, Neoplastic', 'Humans', 'Male', 'NF-kappa B', 'Neoplasm Invasiveness', 'Pancreatic Neoplasms', 'Tumor Cells, Cultured']
| 26,373,617
|
[['D12.776.395.550.200.170', 'D12.776.543.550.200.140', 'D12.776.624.301.249', 'D23.050.285.439.249', 'D23.050.301.350.150'], ['A11.329.228.105'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['G04.161.750', 'G07.345.249.410.750'], ['E05.481.500.374'], ['C23.550.291.656'], ['G02.111.240', 'G05.308.200', 'G07.690.773.937'], ['G05.308.370'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D05.500.672', 'D12.776.260.600', 'D12.776.660.600', 'D12.776.930.600'], ['C04.697.645', 'C23.550.727.645'], ['C04.588.274.761', 'C04.588.322.475', 'C06.301.761', 'C06.689.667', 'C19.344.421'], ['A11.251.860']]
|
['Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Sodium hydrogen exchangers contribute to arenavirus cell entry.
|
Several arenaviruses, chiefly Lassa virus (LASV), cause hemorrhagic fever (HF) disease in humans and pose a great public health concern in the regions in which they are endemic. Moreover, evidence indicates that the worldwide-distributed prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogen of clinical significance. The limited existing armamentarium to combat human-pathogenic arenaviruses underscores the importance of developing novel antiarenaviral drugs, a task that would be facilitated by the identification and characterization of virus-host cell factor interactions that contribute to the arenavirus life cycle. A genome-wide small interfering RNA (siRNA) screen identified sodium hydrogen exchanger 3 (NHE3) as required for efficient multiplication of LCMV in HeLa cells, but the mechanisms by which NHE activity contributed to the life cycle of LCMV remain unknown. Here we show that treatment with the NHE inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA) resulted in a robust inhibition of LCMV multiplication in both rodent (BHK-21) and human (A549) cells. EIPA-mediated inhibition was due not to interference with virus RNA replication, gene expression, or budding but rather to a blockade of virus cell entry. EIPA also inhibited cell entry mediated by the glycoproteins of the HF arenaviruses LASV and Junin virus (JUNV). Pharmacological and genetic studies revealed that cell entry of LCMV in A549 cells depended on actin remodeling and Pak1, suggesting a macropinocytosis-like cell entry pathway. Finally, zoniporide, an NHE inhibitor being explored as a therapeutic agent to treat myocardial infarction, inhibited LCMV propagation in culture cells. Our findings indicate that targeting NHEs could be a novel strategy to combat human-pathogenic arenaviruses.
|
['Animals', 'Arenavirus', 'Blotting, Western', 'Cell Line', 'Humans', 'Membrane Fusion', 'Sodium-Hydrogen Exchangers']
| 24,173,224
|
[['B01.050'], ['B04.820.480.500.070'], ['E05.196.401.143', 'E05.301.300.096', 'E05.478.566.320.200', 'E05.601.262', 'E05.601.470.320.200'], ['A11.251.210'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G04.575'], ['D12.776.157.530.450.162.775', 'D12.776.157.530.937.703', 'D12.776.543.550.190.775', 'D12.776.543.585.450.162.775', 'D12.776.543.585.937.828']]
|
['Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Outcome of liver transplantation in patients with hereditary bleeding disorders: a single centre UK experience.
|
INTRODUCTION: Patients with hereditary bleeding disorders who have developed end-stage liver disease as a consequence of hepatitis C infection (HCV) acquired from factor concentrates prior to the introduction of viral inactivation continue to be referred for liver transplantation.METHODS: A retrospective review of case notes and electronic records was performed on all patients with bleeding disorders who have undergone liver transplantation at the University Hospital Birmingham (UHB).RESULTS: Between 1990 and 2014, 35 liver transplants have been performed in 33 patients with hereditary bleeding disorders. The indication for transplantation was mainly end-stage liver disease secondary to HCV. Five patients had human immunodeficiency virus (HIV) co-infection. No excess mortality due to bleeding occurred in the peri or postoperative period. Median overall survival post transplant is 9.7 years. Overall survival rates at 1, 3 and 5 years are 90%, 72% and 64% respectively. The predominant cause of mortality was liver failure secondary to either recurrent HCV or recurrent hepatocellular carcinoma (HCC). The median overall survival in patients with HIV co-infection is shorter than in those with mono-infection but this is not statistically significant. Patients with a pre-existing HCC had a statistically significant shorter survival (2.4 years vs. 13.6 years, P = 0.007).CONCLUSION: Liver transplantation has become an accepted treatment option for patients with hereditary bleeding disorders and HCV associated end-stage liver disease with survival rates similar to non-bleeding disorder patients.
|
['Adult', 'Aged', 'Carcinoma, Hepatocellular', 'End Stage Liver Disease', 'Hemophilia A', 'Hemophilia B', 'Hepatitis C', 'Humans', 'Liver Neoplasms', 'Liver Transplantation', 'Middle Aged', 'Postoperative Complications', 'Retrospective Studies', 'Survival Analysis', 'United Kingdom']
| 26,931,744
|
[['M01.060.116'], ['M01.060.116.100'], ['C04.557.470.200.025.255', 'C04.588.274.623.160', 'C06.301.623.160', 'C06.552.697.160'], ['C06.552.308.500.177'], ['C15.378.100.100.500', 'C15.378.100.141.500', 'C15.378.463.500', 'C16.320.099.500'], ['C15.378.100.100.510', 'C15.378.100.141.510', 'C15.378.463.510', 'C16.320.099.510', 'C16.320.322.235'], ['C01.221.250.750', 'C01.925.440.440', 'C01.925.782.350.350', 'C06.552.380.705.440'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.274.623', 'C06.301.623', 'C06.552.697'], ['E02.095.147.725.490', 'E04.210.650', 'E04.936.450.490', 'E04.936.580.490'], ['M01.060.116.630'], ['C23.550.767'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.740.998', 'N05.715.360.750.795', 'N06.850.520.830.998'], ['Z01.542.363']]
|
['Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Knockdown of DISC1 by in utero gene transfer disturbs postnatal dopaminergic maturation in the frontal cortex and leads to adult behavioral deficits.
|
Adult brain function and behavior are influenced by neuronal network formation during development. Genetic susceptibility factors for adult psychiatric illnesses, such as Neuregulin-1 and Disrupted-in-Schizophrenia-1 (DISC1), influence adult high brain functions, including cognition and information processing. These factors have roles during neurodevelopment and are likely to cooperate, forming pathways or "signalosomes." Here we report the potential to generate an animal model via in utero gene transfer in order to address an important question of how nonlethal deficits in early development may affect postnatal brain maturation and high brain functions in adulthood, which are impaired in various psychiatric illnesses such as schizophrenia. We show that transient knockdown of DISC1 in the pre- and perinatal stages, specifically in a lineage of pyramidal neurons mainly in the prefrontal cortex, leads to selective abnormalities in postnatal mesocortical dopaminergic maturation and behavioral abnormalities associated with disturbed cortical neurocircuitry after puberty.
|
['Analysis of Variance', 'Animals', 'Antipsychotic Agents', 'Behavior, Animal', 'Cell Differentiation', 'Cell Lineage', 'Chromatography, High Pressure Liquid', 'Clozapine', 'Dopamine', 'Dopamine Agents', 'Electrophysiology', 'Exploratory Behavior', 'Frontal Lobe', 'Gene Transfer Techniques', 'Immunohistochemistry', 'Methamphetamine', 'Mice', 'Microdialysis', 'Motor Activity', 'Nerve Net', 'Nerve Tissue Proteins', 'Neurons', 'RNA Interference', 'Recognition, Psychology', 'Sensory Gating', 'Spatial Behavior']
| 20,188,653
|
[['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['B01.050'], ['D27.505.696.277.950.040', 'D27.505.954.427.210.950.040', 'D27.505.954.427.700.872.331'], ['F01.145.113'], ['G04.152'], ['G04.172', 'G07.345.500.325.180.500', 'G08.686.155', 'G08.686.784.170.104.249'], ['E05.196.181.400.300'], ['D03.633.300.240.220'], ['D02.092.211.215.406', 'D02.092.311.342', 'D02.455.426.559.389.657.166.175.342'], ['D27.505.519.625.150', 'D27.505.696.577.150'], ['H01.158.344.528', 'H01.158.782.236'], ['F01.145.387', 'F01.658.370'], ['A08.186.211.200.885.287.500.270'], ['E05.393.350'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D02.092.471.683.152.619'], ['B01.050.150.900.649.313.992.635.505.500'], ['E05.196.353.500'], ['F01.145.632', 'G11.427.410.698'], ['A08.511'], ['D12.776.631'], ['A08.675', 'A11.671'], ['G05.308.203.374.790'], ['F02.463.425.540.706'], ['G11.561.794'], ['F01.145.875']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Disciplines and Occupations [H]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 1
| 0
|
Hypermodified nucleosides in the anticodon of tRNALys stabilize a canonical U-turn structure.
|
Modified nucleosides in the anticodon domain of Escherichia coli tRNA(Lys) are necessary for high-affinity codon recognition and reading frame maintenance. Human tRNA(Lys,3) is the specific primer for HIV-1 reverse transcriptase and also requires nucleoside modification for proper function. We now present NMR solution structures for the fully modified 17-nucleotide E. coli tRNA(Lys) anticodon stem-loop domain (ASL). NMR data were also collected for several partially modified ASLs, revealing the contributions each modified nucleoside (mnm(5)s(2)U34, t(6)A37, and psi39) makes in transforming the disordered, unmodified tRNA ASL into the highly ordered native structure. The solution structure of the native ASL domain provides insight into longstanding questions regarding both wobble position modification and the nearly ubiquitous t(6)A37 found in tRNAs with an adjacent U at position 36. Native tRNA(Lys) has a U-turn structure similar to the yeast tRNA(Phe) crystal structure, unlike previously proposed "unconventional" anticodon structures characterized by stable interactions between mnm(5)s(2)U-34 and t(6)A-37.
|
['Anticodon', 'Circular Dichroism', 'Escherichia coli', 'Humans', 'Models, Molecular', 'Nuclear Magnetic Resonance, Biomolecular', 'Nucleic Acid Conformation', 'Nucleosides', 'Phosphorus Isotopes', 'Protons', 'RNA, Bacterial', 'RNA, Fungal', 'RNA, Transfer, Lys', 'Saccharomyces cerevisiae', 'Spectrophotometry, Ultraviolet', 'Structure-Activity Relationship', 'Sulfur', 'Thermodynamics', 'Uridine']
| 11,027,137
|
[['D13.444.735.757.286', 'G05.360.335.060'], ['E05.196.867.151'], ['B03.440.450.425.325.300', 'B03.660.250.150.180.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.599.595'], ['E05.196.867.519.550'], ['G02.111.570.820.486', 'G05.360.580'], ['D09.408.595', 'D13.570'], ['D01.268.666.500', 'D01.496.669'], ['D01.248.497.300.459.700', 'D01.268.406.750', 'D01.362.340.750', 'G01.249.660.500'], ['D13.444.735.473'], ['D13.444.735.500'], ['D13.444.735.757.700.510'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['E05.196.712.726.802', 'E05.196.867.826.802'], ['G02.111.830', 'G07.690.773.997'], ['D01.268.185.900'], ['G01.906'], ['D03.383.742.680.852', 'D13.570.685.852', 'D13.570.800.892']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Reflexes to sacral parasympathetic neurones concerned with micturition in the cat.
|
1. Reflexes to sacral parasympathetic neurones were studied by electrophysiological techniques in decerebrate, in chloralose-anaesthetized, and in chronic spinal cats.2. Excitatory reflexes from pelvic nerve and sacral somatic afferent nerve fibres were present before and after chronic transection of the spinal cord, but the latencies differed markedly. It was concluded that the long-latency reflexes observed when the spinal cord was intact involved long-loop reflexes to the brain-stem. The weak, short-latency reflexes in the chronic spinal cat were never observed when the spinal cord was intact and could be due to reorganized spinal connexions. The short-latency reflexes are probably unimportant in normal micturition.3. Stimulation of afferent fibres in the pelvic or sacral somatic nerves produced short-latency inhibitory post-synaptic potentials (IPSPs) and inhibition of discharges in parasympathetic neurones. This inhibition was due to a spinal reflex.4. A local reflex was demonstrated in the pelvic plexus. This was probably a cholinergic axon reflex, but the remote possibility that it was a local cholinergic reflex involving sensory neurones in the bladder wall has not been excluded.
|
['Animals', 'Cats', 'Chloralose', 'Cordotomy', 'Decerebrate State', 'Electrophysiology', 'Membrane Potentials', 'Neurons', 'Parasympathetic Nervous System', 'Reflex', 'Sacrum', 'Urinary Bladder', 'Urination']
| 5,248,885
|
[['B01.050'], ['B01.050.150.900.649.313.750.377.750.250.125'], ['D02.033.455.250.130.150', 'D09.408.348.150'], ['E04.525.210.210'], ['C10.597.305', 'C23.888.592.298'], ['H01.158.344.528', 'H01.158.782.236'], ['G01.154.535', 'G04.580', 'G07.265.675', 'G11.561.570'], ['A08.675', 'A11.671'], ['A08.800.050.600'], ['E01.370.376.550.650', 'E01.370.600.550.650', 'F02.830.702', 'G11.561.731'], ['A02.835.232.834.717'], ['A05.810.890'], ['G08.852.880']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Disciplines and Occupations [H]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Galacturonic-acid-induced increase of superoxide production in red tide phytoplankton Chattonella marina and Heterosigma akashiwo.
|
Red tide phytoplankton, Chattonella marina and Heterosigma akashiwo, are known to generate superoxide anion (O2-). We found that galacturonic acid (GaLUA) stimulated C. marina and H. akashiwo to generate increased amounts of O2-. Since such effect was not observed in any other monosaccharides tested, our results suggest that the binding of GalUA to specific sites on the flagellate cell surface may induce the increase of 02- production.
|
['Culture Media', 'Hexuronic Acids', 'Imidazoles', 'Phytoplankton', 'Pyrazines', 'Rhodophyta', 'Superoxide Dismutase', 'Superoxides']
| 10,830,520
|
[['D27.720.470.305', 'E07.206'], ['D02.241.081.844.915.400', 'D02.241.152.811.400', 'D02.241.511.902.915.400', 'D09.811.922.400'], ['D03.383.129.308'], ['B05.080.500.600'], ['D03.383.679'], ['B01.650.700'], ['D08.811.682.881'], ['D01.248.497.158.685.750.850', 'D01.339.431.374.850', 'D01.650.550.750.800', 'D02.389.338.732']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Cord blood nesfatin-1 and apelin-36 levels in gestational diabetes mellitus.
|
To assess maternal serum and cord blood apelin-36 and nesfatin-1 concentrations in pregnant women with and without gestational diabetes mellitus (GDM). Thirty pregnant women with GDM and 30 gestational age matched healthy pregnant subjects participated to the study. Maternal serum and cord blood nesfatin-1 and apelin-36 levels were measured with ELISA, at the time of birth. The relationships between maternal serum and cord blood nesfatin-1 and apelin-36 levels, anthropometric and metabolic parameters were also assessed. Maternal serum apelin-36 levels were found higher (13.5 ± 8.3 vs. 9.6 ± 5.9 ng/ml, P = 0.001) and nesfatin-1 levels were found lower (5.5 ± 8.1 vs. 8.1 ± 23.9 ng/ml, P = 0.001) in patients with GDM compared with control pregnant women. However, the cord blood apelin-36 levels (8.8 ± 4.3 and 8.2 ± 1.9 ng/ml, P = 0.618) and nesfatin-1 levels (5.4 ± 4.0 and 6.2 ± 10.3 ng/ml, P = 0.688) were similar in the GDM and control groups, respectively. Maternal serum apelin-36 and nesfatin-1 levels correlated positively with their respective cord blood levels. Maternal serum and cord blood apelin-36 levels correlated negatively with the gestational age and birth weight. Similarly maternal serum and cord blood nesfatin-1 levels correlated negatively with the gestational age, but there was no correlation with the birth weight. We did not find a correlation between maternal serum apelin-36 and nesfatin-1 levels, maternal age, BMI, fasting glucose, fasting insulin, and HOMA-IR. Also cord blood apelin-36 and nesfatin-1 levels did not correlate with the maternal age, BMI, HOMA-IR, cord blood glucose, and cord blood insulin levels. Our results indicate that apelin-36 concentrations increase and nesfatin-1 concentrations decrease in maternal serum of women with GDM.
|
['Adult', 'Apelin', 'Biomarkers', 'Birth Weight', 'Calcium-Binding Proteins', 'DNA-Binding Proteins', 'Diabetes, Gestational', 'Female', 'Fetal Blood', 'Gestational Age', 'Humans', 'Infant, Newborn', 'Insulin', 'Intercellular Signaling Peptides and Proteins', 'Male', 'Nerve Tissue Proteins', 'Nucleobindins', 'Pilot Projects', 'Postpartum Period', 'Pregnancy']
| 22,203,468
|
[['M01.060.116'], ['D12.644.276.150', 'D23.529.134'], ['D23.101'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['D12.776.157.125'], ['D12.776.260'], ['C13.703.170', 'C18.452.394.750.448', 'C19.246.200'], ['A12.207.152.200', 'A15.145.300', 'A16.378.200'], ['G07.345.500.325.235.968', 'G08.686.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['D12.644.276', 'D12.776.467', 'D23.529'], ['D12.776.631'], ['D12.776.157.125.588', 'D12.776.260.623'], ['E05.318.372.750', 'E05.337.737', 'N05.715.360.330.720', 'N06.850.520.450.720'], ['G08.686.702'], ['G08.686.784.769']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Organisms [B]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Rapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis.
|
We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Chromosomal DNA from dinger zones (visible subsurface zones of leptospiral growth) of first semisolid subcultures of field isolates was also isolated and characterized, thus greatly speeding up the diagnostic process.
|
['Chromosomes, Bacterial', 'DNA Restriction Enzymes', 'DNA, Bacterial', 'Deoxyribonuclease EcoRI', 'Leptospira interrogans', 'Ultracentrifugation']
| 3,001,135
|
[['A11.284.187.190', 'A20.812', 'G05.360.162.190'], ['D08.811.150.280', 'D08.811.277.352.335.350.300', 'D08.811.277.352.355.325.300'], ['D13.444.308.212'], ['D08.811.150.280.260.300', 'D08.811.277.352.335.350.300.260.250', 'D08.811.277.352.355.325.300.260.250'], ['B03.440.400.425.475.475.400', 'B03.851.475.475.400'], ['E05.181.724', 'E05.196.941']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
TwinsUK: The UK Adult Twin Registry Update.
|
TwinsUK is the largest cohort of community-dwelling adult twins in the UK. The registry comprises over 14,000 volunteer twins (14,838 including mixed, single and triplets); it is predominantly female (82%) and middle-aged (mean age 59). In addition, over 1800 parents and siblings of twins are registered volunteers. During the last 27 years, TwinsUK has collected numerous questionnaire responses, physical/cognitive measures and biological measures on over 8500 subjects. Data were collected alongside four comprehensive phenotyping clinical visits to the Department of Twin Research and Genetic Epidemiology, King's College London. Such collection methods have resulted in very detailed longitudinal clinical, biochemical, behavioral, dietary and socioeconomic cohort characterization; it provides a multidisciplinary platform for the study of complex disease during the adult life course, including the process of healthy aging. The major strength of TwinsUK is the availability of several 'omic' technologies for a range of sample types from participants, which includes genomewide scans of single-nucleotide variants, next-generation sequencing, metabolomic profiles, microbiomics, exome sequencing, epigenetic markers, gene expression arrays, RNA sequencing and telomere length measures. TwinsUK facilitates and actively encourages sharing the 'TwinsUK' resource with the scientific community - interested researchers may request data via the TwinsUK website (http://twinsuk.ac.uk/resources-for-researchers/access-our-data/) for their own use or future collaboration with the study team. In addition, further cohort data collection is planned via the Wellcome Open Research gateway (https://wellcomeopenresearch.org/gateways). The current article presents an up-to-date report on the application of technological advances, new study procedures in the cohort and future direction of TwinsUK.
|
['Adolescent', 'Adult', 'Aged', 'Aged, 80 and over', 'Diseases in Twins', 'Female', 'Genetic Markers', 'Genome-Wide Association Study', 'Humans', 'Incidence', 'Longitudinal Studies', 'Male', 'Metabolome', 'Metagenome', 'Middle Aged', 'Registries', 'Twins', 'United Kingdom', 'Young Adult']
| 31,526,404
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['C23.550.291.750'], ['D23.101.387', 'G05.695.450'], ['E05.318.370.392', 'E05.318.416.249', 'E05.393.385.500', 'E05.393.522.500', 'E05.393.760.640.500', 'N06.850.520.445.392', 'N06.850.520.470.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.308.985.525.375', 'N01.224.935.597.500', 'N06.850.505.400.975.525.375', 'N06.850.520.308.985.525.375'], ['E05.318.372.500.750.500', 'N05.715.360.330.500.750.500', 'N06.850.520.450.500.750.500'], ['G03.500'], ['G05.360.340.550'], ['M01.060.116.630'], ['E05.318.308.970', 'N04.452.859.819', 'N05.715.360.300.715.700', 'N06.850.520.308.970'], ['M01.438.873'], ['Z01.542.363'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Unrelated cord blood transplantation after myeloablative conditioning regimen in adolescent and young adult patients with hematologic malignancies: a single institute analysis.
|
We report the results of unrelated cord blood transplantation (CBT) after myeloablative conditioning regimen in 16 patients with hematologic malignancies from 15 to 20 years old. The median times of myeloid and platelet engraftment were 21 and 38 days, respectively. The cumulative incidences of acute graft-vs-host disease (GVHD) was 62.0%, all of which were grade I or II, and that of extensive-type chronic GVHD was 12.5%. The probabilities of overall and disease-free survival at 3 years were 68.2% and 48.6%, respectively, comparable to adult or childhood cases. Adolescents and young adult patients with hematologic malignancies who have no HLA-matched adult donors could be considered as candidates for CBT.
|
['Adolescent', 'Adult', 'Cord Blood Stem Cell Transplantation', 'Disease-Free Survival', 'Female', 'Graft vs Host Disease', 'Hematologic Neoplasms', 'Histocompatibility Testing', 'Humans', 'Male', 'Retrospective Studies', 'Survival Rate', 'Tissue Donors', 'Transplantation Conditioning', 'Young Adult']
| 21,982,638
|
[['M01.060.057'], ['M01.060.116'], ['E02.095.147.500.500.312', 'E04.936.225.687.312'], ['E01.789.800.190', 'E05.318.740.998.300', 'N04.761.559.590.800.190', 'N05.715.360.575.575.800.190', 'N05.715.360.750.795.300', 'N06.850.520.830.998.300'], ['C20.452'], ['C04.588.448', 'C15.378.400'], ['E01.370.225.812.385', 'E05.200.812.385', 'E05.478.594.385'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E05.318.308.985.550.900', 'N01.224.935.698.826', 'N06.850.505.400.975.550.900', 'N06.850.520.308.985.550.900'], ['M01.898'], ['E02.095.465.425.450.800', 'E05.478.610.800'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
A novel insight into the mechanism of mammalian selenoprotein synthesis.
|
The amino acid selenocysteine is encoded by UGA, usually a stop codon, thus requiring a specialized machinery to enable its incorporation into selenoproteins. The machinery comprises the tRNA(Sec), a 3'-UTR mRNA stem-loop termed SElenoCysteine Insertion Sequence (SECIS), which is mandatory for recoding UGA as a Sec codon, the SECIS Binding Protein 2 (SBP2), and other proteins. Little is known about the molecular mechanism and, in particular, when, where, and how the SECIS and SBP2 contact the ribosome. Previous work by others used the isolated SECIS RNA to address this question. Here, we developed a novel approach using instead engineered minimal selenoprotein mRNAs containing SECIS elements derivatized with photoreactive groups. By cross-linking experiments in rabbit reticulocyte lysate, new information could be gained about the SBP2 and SECIS contacts with components of the translation machinery at various translation steps. In particular, we found that SBP2 was bound only to the SECIS in 48S pre-initiation and 80S pretranslocation complexes. In the complex where the Sec-tRNA(Sec) was accommodated to the A site but transpeptidation was blocked, SBP2 bound the ribosome and possibly the SECIS element as well, and the SECIS had flexible contacts with the 60S ribosomal subunit involving several ribosomal proteins. Altogether, our findings led to broadening our understanding about the unique mechanism of selenocysteine incorporation in mammals.
|
["3' Untranslated Regions", 'Animals', 'Base Sequence', 'Humans', 'Molecular Sequence Data', 'Nucleic Acid Conformation', 'RNA, Messenger', 'RNA, Transfer, Amino Acid-Specific', 'RNA-Binding Proteins', 'Rabbits', 'Recombinant Fusion Proteins', 'Reticulocytes', 'Ribosomes', 'Selenoproteins']
| 23,788,723
|
[['D13.444.735.544.875.880', 'D13.444.735.790.878.880', 'G05.360.340.024.220.880.880', 'G05.360.340.024.340.137.910.880'], ['B01.050'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['L01.453.245.667'], ['G02.111.570.820.486', 'G05.360.580'], ['D13.444.735.544'], ['D13.444.735.757.700'], ['D12.776.157.725', 'D12.776.664.962'], ['B01.050.150.900.649.313.968.700'], ['D12.776.828.300'], ['A11.118.290.760', 'A11.148.790', 'A11.443.240.665', 'A15.145.229.334.760', 'A15.378.316.790'], ['A11.284.430.214.190.875.811'], ['D12.776.864']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Information Science [L]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Differential dependence on nuclear factor-êB-inducing kinase among natural killer T-cell subsets in their development.
|
Natural killer T cells (NKT cells) are comprised of several subsets. However, the possible differences in their developmental mechanisms have not been fully investigated. To evaluate the dependence of some NKT subpopulations on nuclear factor-êB-inducing kinase (NIK) for their generation, we analysed the differentiation of NKT cells, dividing them into subsets in various tissues of alymphoplasia (aly/aly), a mutant mouse strain that lacks functional NIK. The results indicated that the efficient differentiation of both invariant NKT (iNKT) and non-iNKT cells relied on NIK expression in non-haematopoietic cells; however, the dependence of non-iNKT cells was lower than that of iNKT cells. Especially, the differentiation of CD8(+) non-iNKT cells was markedly resistant to the aly mutation. The proportion of two other NKT cell subsets, NK1.1(+) ãä T cells and NK1.1(-) iNKT cells, was also significantly reduced in aly/aly mice, and this defect in their development was reversed in wild-type host mice given aly/aly bone marrow cells. In exerting effector functions, NIK in NKT-áâ cells appeared dispensable, as NIK-deficient NKT-áâ cells could secrete interleukin-4 or interferon-ã and exhibit cytolytic activity at a level comparable to that of aly/+ NKT-áâ cells. Collectively, these results imply that the NIK in thymic stroma may be critically involved in the differentiation of most NKT cell subsets (although the level of NIK dependence may vary among the subsets), and also that NIK in NKT-áâ cells may be dispensable for their effector function.
|
['Animals', 'CD8-Positive T-Lymphocytes', 'Cell Differentiation', 'Cells, Cultured', 'Interferon-gamma', 'Interleukin-15', 'Interleukin-4', 'Interleukin-7', 'Mice', 'Mice, Inbred C57BL', 'Mice, Knockout', 'Natural Killer T-Cells', 'Protein-Serine-Threonine Kinases', 'Receptors, Antigen, T-Cell, alpha-beta', 'Receptors, Antigen, T-Cell, gamma-delta', 'T-Lymphocyte Subsets']
| 25,988,531
|
[['B01.050'], ['A11.118.637.555.567.569.220', 'A15.145.229.637.555.567.569.220', 'A15.382.490.555.567.569.220'], ['G04.152'], ['A11.251'], ['D12.644.276.374.440.893', 'D12.644.276.374.480.615.350', 'D12.776.467.374.440.893', 'D12.776.467.374.480.615.350', 'D23.529.374.440.893', 'D23.529.374.480.615.350'], ['D12.644.276.374.465.515', 'D12.776.467.374.465.515', 'D23.529.374.465.515'], ['D12.644.276.374.465.186', 'D12.776.467.374.465.178', 'D23.529.374.465.186'], ['D12.644.276.374.465.246', 'D12.776.467.374.465.224', 'D23.529.374.465.246'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.050.199.520.520.420', 'B01.050.150.900.649.313.992.635.505.500.400.420'], ['B01.050.050.136.500.500', 'B01.050.150.900.649.313.992.635.505.500.550.455', 'B01.050.150.900.649.313.992.635.505.500.800.500'], ['A11.118.637.555.567.569.290', 'A15.145.229.637.555.567.569.360', 'A15.382.490.555.567.569.470'], ['D08.811.913.696.620.682.700'], ['D12.776.543.750.705.816.824.825'], ['D12.776.543.750.705.816.824.830'], ['A11.118.637.555.567.550.500', 'A11.118.637.555.567.569.500', 'A15.145.229.637.555.567.550.500', 'A15.145.229.637.555.567.569.500', 'A15.382.490.555.567.550.500', 'A15.382.490.555.567.569.500']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Hsp90 is an essential regulator of EphA2 receptor stability and signaling: implications for cancer cell migration and metastasis.
|
A subset of Eph receptors and their corresponding ligands are commonly expressed in tumor cells where they mediate biological processes such as cell migration and adhesion, whereas their expression in endothelial cells promotes angiogenesis. In particular, the tumor-specific up-regulation of EphA2 confers properties of increased cellular motility, invasiveness, tumor angiogenesis, and tumor progression, and its overexpression correlates with poor prognosis in several cancer types. The cellular chaperone Hsp90 also plays a significant role in regulating cell migration and angiogenesis, although the full repertoire of motility driving proteins dependent on Hsp90 function remain poorly defined. We explored the hypothesis that Hsp90 may regulate the activity of EphA2 and examined the potential relationship between EphA2 receptor signaling and chaperone function. We show that geldanamycin, an Hsp90 antagonist, dramatically destabilizes newly synthesized EphA2 protein and diminishes receptor levels in a proteasome-dependent pathway. In addition, geldanamycin treatment impairs EphA2 signaling, as evidenced by a decrease in ligand-dependent receptor phosphorylation and subsequent cell rounding. Therefore, Hsp90 exerts a dual role in regulating the stability of nascent EphA2 protein and maintaining the signaling capacity of the mature receptor. Our findings also suggest that the geldanamycin-dependent mitigation of EphA2 signaling in receptor-overexpressing cancer cells may be sufficient to recapitulate the antimotility effects of this drug. Finally, the identification of a pharmacologic approach to suppress EphA2 expression and signaling highlights the attractive possibility that Hsp90 inhibitors may have clinical utility in antagonizing EphA2-dependent tumorigenic progression.
|
['Cell Line', 'Cell Line, Tumor', 'Cell Movement', 'HSP90 Heat-Shock Proteins', 'Humans', 'Microscopy, Fluorescence', 'Neoplasm Metastasis', 'Neoplasms', 'Phosphorylation', 'Protein Multimerization', 'Protein Stability', 'Protein Structure, Tertiary', 'Receptor, EphA2', 'Signal Transduction', 'Ubiquitination']
| 19,567,782
|
[['A11.251.210'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['D12.776.580.216.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['E01.370.350.515.458', 'E05.595.458'], ['C04.697.650', 'C23.550.727.650'], ['C04'], ['G02.111.665', 'G02.607.780', 'G03.796'], ['G02.111.694'], ['G02.111.700'], ['G02.111.570.820.709.610'], ['D08.811.913.696.620.682.725.400.850.100', 'D12.776.543.750.630.500.100'], ['G02.111.820', 'G04.835'], ['G02.111.660.871.790.600.925', 'G02.111.691.600.775', 'G03.734.871.790.600.831', 'G05.308.670.600.831']]
|
['Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Inter-species grafting caused extensive and heritable alterations of DNA methylation in Solanaceae plants.
|
BACKGROUND: Grafting has been extensively used to enhance the performance of horticultural crops. Since Charles Darwin coined the term "graft hybrid" meaning that asexual combination of different plant species may generate products that are genetically distinct, highly discrepant opinions exist supporting or against the concept. Recent studies have documented that grafting enables exchanges of both RNA and DNA molecules between the grafting partners, thus providing a molecular basis for grafting-induced genetic variation. DNA methylation is known as prone to alterations as a result of perturbation of internal and external conditions. Given characteristics of grafting, it is interesting to test whether the process may cause an alteration of this epigenetic marker in the grafted organismal products.METHODOLOGY/PRINCIPAL FINDINGS: We analyzed relative global DNA methylation levels and locus-specific methylation patterns by the MSAP marker and locus-specific bisulfite-sequencing in the seed plants (wild-type controls), self- and hetero-grafted scions/rootstocks, selfed progenies of scions and their seed-plant controls, involving three Solanaceae species. We quantified expression of putative genes involved in establishing and/or maintaining DNA methylation by q-(RT)-PCR. We found that (1) hetero-grafting caused extensive alteration of DNA methylation patterns in a locus-specific manner, especially in scions, although relative methylation levels remain largely unaltered; (2) the altered methylation patterns in the hetero-grafting-derived scions could be inherited to sexual progenies with some sites showing further alterations or revisions; (3) hetero-grafting caused dynamic changes in steady-state transcript abundance of genes encoding for a set of enzymes functionally relevant to DNA methylation.CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that inter-species grafting in plants could produce extensive and heritable alterations in DNA methylation. We suggest that these readily altered, yet heritable, epigenetic modifications due to interspecies hetero-grafting may shed one facet of insight into the molecular underpinnings for the still contentious concept of graft hybrid.
|
['DNA Methylation', 'DNA, Plant', 'Molecular Sequence Data', 'Solanaceae']
| 23,614,002
|
[['G02.111.035.538.161', 'G02.111.218', 'G03.059.538.161', 'G05.206'], ['D13.444.308.435'], ['L01.453.245.667'], ['B01.650.940.800.575.912.250.908.500']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Information Science [L]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Yeast RNA polymerase III. Chromatographic, catalytic and DNA-binding properties are highly dependent on the type of anion.
|
The chromatographic, catalytic and DNA-binding properties of yeast RNA polymerase III are highly affected by both concentration and type of salt. The type of anion is an especially important modulating factor for the enzymological properties of the enzyme. When acetate or sulfate anions are substituted for chloride anions, RNA polymerase III exhibits a higher affinity for DEAE-Sephadex A25, becomes able to transcribe DNA at relatively high ionic strength and shows a significant increase in the binding strength to DNA. A quantitative analysis of the binding of the enzyme to single-stranded DNA shows that the number of ionic contacts in the complex is not affected by the type of anion, but the nonionic contribution to the binding constant is significantly increased when acetate is substituted for chloride.
|
['Anions', 'Catalysis', 'Centrifugation, Density Gradient', 'Chemical Phenomena', 'Chemistry', 'Chromatography, Affinity', 'Chromatography, Ion Exchange', 'DNA', 'DNA-Directed RNA Polymerases', 'Enzyme Activation', 'Osmolar Concentration', 'Protein Binding', 'RNA Polymerase III', 'Saccharomyces cerevisiae', 'Templates, Genetic']
| 6,376,122
|
[['D01.248.497.158'], ['G02.130'], ['E05.181.724.336', 'E05.196.941.336'], ['G02'], ['H01.181'], ['E05.196.181.400.170'], ['E05.196.181.400.383'], ['D13.444.308'], ['D08.811.913.696.445.735.270'], ['G02.111.263', 'G03.328'], ['G02.640'], ['G02.111.679', 'G03.808'], ['D08.811.913.696.445.735.270.775'], ['B01.300.107.795.785.800', 'B01.300.930.705.655'], ['G05.360.840']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Synthesis of D- and L-phenylalanine derivatives by phenylalanine ammonia lyases: a multienzymatic cascade process.
|
The synthesis of substituted D-phenylalanines in high yield and excellent optical purity, starting from inexpensive cinnamic acids, has been achieved with a novel one-pot approach by coupling phenylalanine ammonia lyase (PAL) amination with a chemoenzymatic deracemization (based on stereoselective oxidation and nonselective reduction). A simple high-throughput solid-phase screening method has also been developed to identify PALs with higher rates of formation of non-natural D-phenylalanines. The best variants were exploited in the chemoenzymatic cascade, thus increasing the yield and ee value of the D-configured product. Furthermore, the system was extended to the preparation of those L-phenylalanines which are obtained with a low ee value using PAL amination.
|
['Amination', 'Anabaena variabilis', 'Chemistry Techniques, Synthetic', 'Oxidation-Reduction', 'Phenylalanine', 'Phenylalanine Ammonia-Lyase', 'Stereoisomerism']
| 25,728,350
|
[['G02.111.053', 'G02.607.110', 'G03.068'], ['B03.280.100.900', 'B03.440.475.100.100.900', 'B03.585.051.900'], ['E05.197', 'J01.897.836.249'], ['G02.700', 'G03.295.531'], ['D12.125.072.050.685', 'D12.125.142.666'], ['D08.811.520.232.400.700'], ['G02.607.445.682']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Chemicals and Drugs [D]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Utilization of a virtual patient for advanced assessment of student performance in pain management.
|
BACKGROUND AND PURPOSE: To assess student performance and achievement of course objectives following the integration of a virtual patient case designed to promote active, patient-centered learning in a required pharmacy course.EDUCATIONAL ACTIVITY AND SETTING: DecisionSim™ (Kynectiv, Inc., Chadsford, PA), a dynamic virtual patient platform, was used to implement an interactive patient case to augment pain management material presented during a didactic session in a pharmacotherapy course. Simulation performance data were collected and analyzed. Student exam performance on pain management questions was compared to student exam performance on nearly identical questions from a prior year when a paper-based case was used instead of virtual patient technology.FINDINGS: Students who performed well on the virtual patient case performed better on exam questions related to patient assessment (p = 0.0244), primary pharmacological therapy (p = 0.0001), and additional pharmacological therapy (p = 0.0001). Overall exam performance did not differ between the two groups. However, students with exposure to the virtual patient case demonstrated significantly better performance on higher level Bloom's Taxonomy questions that required them to create pharmacotherapy regimens (p=0.0005). Students in the previous year (exposed only to a paper patient case) performed better in calculating conversions of opioids for patients (p = 0.0001).SUMMARY: Virtual patient technology may enhance student performance on high-level Bloom's Taxonomy examination questions. This study adds to the current literature demonstrating the value of virtual patient technology as an active-learning strategy.
|
['Adult', 'Curriculum', 'Education, Pharmacy', 'Educational Measurement', 'Female', 'Humans', 'Male', 'Middle Aged', 'Pain Management', 'Patient Simulation', 'Students, Pharmacy']
| 29,233,321
|
[['M01.060.116'], ['I02.158'], ['I02.358.525'], ['I02.399'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E02.745', 'N04.590.607.500'], ['I02.903.847.500'], ['M01.848.769.768']]
|
['Named Groups [M]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Imaging and treating tumor vasculature with targeted radiolabeled carbon nanotubes.
|
Single wall carbon nanotube (SWCNT) constructs were covalently appended with radiometal-ion chelates (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid [DOTA] or desferrioxamine B [DFO]) and the tumor neovascular-targeting antibody E4G10. The E4G10 antibody specifically targeted the monomeric vascular endothelial-cadherin (VE-cad) epitope expressed in the tumor angiogenic vessels. The construct specific activity and blood compartment clearance kinetics were significantly improved relative to corresponding antibodyalone constructs. We performed targeted radioimmunotherapy with a SWCNT-([(225)Ac]DOTA) (E4G10) construct directed at the tumor vasculature in a murine xenograft model of human colon adenocarcinoma (LS174T). The specific construct reduced tumor volume and improved median survival relative to controls. We also performed positron emission tomographic (PET) radioimmunoimaging of the tumor vessels with a SWCNT-([(89)Zr]DFO)(E4G10) construct in the same murine LS174T xenograft model and compared the results to appropriate controls. Dynamic and longitudinal PET imaging of LS174T tumor-bearing mice demonstrated rapid blood clearance (<1 hour) and specific tumor accumulation of the specific construct. Incorporation of the SWCNT scaffold into the construct design permitted us to amplify the specific activity to improve the signal-to-noise ratio without detrimentally impacting the immunoreactivity of the targeting antibody moiety. Furthermore, we were able to exploit the SWCNT pharmacokinetic (PK) profile to favorably alter the blood clearance and provide an advantage for rapid imaging. Near-infrared three-dimensional fluorescent-mediated tomography was used to image the LS174T tumor model, collect antibody-alone PK data, and calculate the number of copies of VE-cad epitope per cell. All of these studies were performed as a single administration of construct and were found to be safe and well tolerated by the murine model. These data have implications that support further imaging and radiotherapy studies using a SWCNT-based platform and focusing on the tumor vessels as the target.
|
['Actinium', 'Adenocarcinoma', 'Animals', 'Cell Line, Tumor', 'Colonic Neoplasms', 'Deferoxamine', 'Heterocyclic Compounds, 1-Ring', 'Humans', 'Male', 'Mice', 'Mice, Nude', 'Nanomedicine', 'Nanotubes, Carbon', 'Neovascularization, Pathologic', 'Positron-Emission Tomography', 'Radioimmunodetection', 'Radioimmunotherapy', 'Radioisotopes', 'Radiopharmaceuticals', 'Xenograft Model Antitumor Assays', 'Zirconium']
| 21,042,424
|
[['D01.268.271.100.033', 'D01.268.556.025', 'D01.496.749.305.100.033', 'D01.552.020.042', 'D01.552.544.025'], ['C04.557.470.200.025'], ['B01.050'], ['A11.251.210.190', 'A11.251.860.180'], ['C04.588.274.476.411.307.180', 'C06.301.371.411.307.180', 'C06.405.249.411.307.180', 'C06.405.469.158.356.180', 'C06.405.469.491.307.180'], ['D02.092.570.394.265', 'D02.241.511.372.265'], ['D03.383'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B01.050.150.900.649.313.992.635.505.500'], ['B01.050.150.900.649.313.992.635.505.500.550.500'], ['H01.603.600', 'J01.897.120.050.625', 'J01.897.520.600.600'], ['D01.268.150.250.500', 'J01.637.512.850.500'], ['C23.550.589.500'], ['E01.370.350.350.800.700', 'E01.370.350.600.350.800.399', 'E01.370.350.710.800.399', 'E01.370.350.825.800.399', 'E01.370.384.730.800.399'], ['E01.370.350.710.710', 'E01.370.384.730.710', 'E05.478.805'], ['E02.095.465.425.750', 'E02.186.750', 'E02.815.520'], ['D01.496.749'], ['D27.505.259.843', 'D27.505.519.871', 'D27.720.470.410.650'], ['E05.337.550.200.900', 'E05.624.850'], ['D01.268.556.950', 'D01.268.956.937', 'D01.552.544.950']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
|
Mid-arm circumference/head circumference ratios for identification of symptomatic LGA, AGA, and SGA newborn infants.
|
Mid-arm circumference/head circumference ratios (MAC/HC) and birth weights obtained in 73 neonates were studied to compare which of these growth measurements could more accurately predict risk of metabolic complications resulting from either acceleration or retardation of fetal growth. The MAC/HC ratio was more sensitive than birth weight in distinguishing symptomatic large for gestational age (LGA) infants who were born to diabetic mothers from other LGA infants who were asymptomatic, and symptomatic from asymptomatic small for gestational age infants. In addition, the MAC/HC ratio identified symptomatic appropriate for gestational age (AGA) infants born to diabetic mothers and AGA infants with signs and symptoms of growth retardation. The MAC/HC is more useful than birth weight in assessing newborn infants at risk for the metabolic complications associated with fetal growth disorders.
|
['Anthropometry', 'Arm', 'Birth Weight', 'Female', 'Fetal Growth Retardation', 'Gestational Age', 'Head', 'Humans', 'Infant, Newborn', 'Infant, Small for Gestational Age', 'Pregnancy', 'Pregnancy in Diabetics', 'Risk']
| 3,734,969
|
[['E01.370.600.024', 'E05.041', 'N06.850.505.200.100'], ['A01.378.800.075'], ['C23.888.144.186', 'E01.370.600.115.100.160.120.186', 'E05.041.124.160.750.149', 'G07.100.100.160.120.186', 'G07.345.249.314.120.186'], ['C13.703.277.370', 'C16.300.390', 'C23.550.393.450'], ['G07.345.500.325.235.968', 'G08.686.320'], ['A01.456'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['M01.060.703.520.460.560'], ['G08.686.784.769'], ['C13.703.726'], ['E05.318.740.600.800', 'G17.680.750', 'N05.715.360.750.625.700', 'N06.850.520.830.600.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Diseases [C]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Investigation of the protective effects of crocin on acrylamide induced small and large intestine damage in rats.
|
We investigated repair of acrylamide (AA) induced damage in intestines by administration of crocin. We used 40 male Wistar rats in four groups of 10 animals: control, AA, crocin, and AA + crocin groups. We investigated biochemical and histological changes to small and large intestine. AA ingestion decreased glutathione (GSH) levels and total antioxidant status (TAS) in the intestine compared to the control group, while superoxide dismutase (SOD) and catalase (CAT) activities, and total oxidant status (TOS) and malondialdehyde (MDA) levels were increased. Villi were shortened and villus degeneration was observed in ileum of the AA group. Degeneration of surface epithelium and Liberk?hn crypts were observed in colon sections. GSH and TAS levels increased after administration of AA together with crocin, while SOD and CAT levels and TOS and MDA levels decreased; significant recovery of histological damage also was observed. We found that crocin exhibits protective effects on AA induced small and large intestine damage by inhibiting oxidative stress.
|
['Acrylamide', 'Animals', 'Antioxidants', 'Carotenoids', 'Glutathione Peroxidase', 'Intestine, Large', 'Male', 'Malondialdehyde', 'Oxidative Stress', 'Rats, Wistar', 'Superoxide Dismutase']
| 29,644,878
|
[['D02.065.122.015', 'D02.241.081.069.094.015'], ['B01.050'], ['D27.505.519.217', 'D27.505.696.706.125', 'D27.720.799.047'], ['D02.455.326.271.665.202', 'D02.455.426.392.368.367.379.249', 'D02.455.849.131', 'D23.767.261'], ['D08.811.682.732.500'], ['A03.556.124.526', 'A03.556.249.249'], ['D02.047.700'], ['G03.673', 'G07.775.750'], ['B01.050.150.900.649.313.992.635.505.700.900'], ['D08.811.682.881']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Blepharophimosis, ptosis and epicanthus inversus].
|
Blepharophimosis, ptosis and epicanthus inversus is a rare well-documented autosomal dominant disorder. Described here is a family with typical features of this syndrome in eleven cases in five generations. Syndrome is discussed and blepharophimosis underlined. Blepharophimosis is also a minor defect in the contest of complex malformation syndromes having different aetiology (mendelian inheritance, chromosomal abnormality, toxic agents). The Authors believe that blepharophimosis, for its clinical evidence, is an important guide sign.
|
['Blepharoptosis', 'Eyelids', 'Female', 'Genes, Dominant', 'Humans', 'Infant', 'Pedigree']
| 6,544,430
|
[['C11.338.204'], ['A01.456.505.420.504', 'A09.371.337'], ['G05.360.340.024.340.240', 'G05.420.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['E05.393.673']]
|
['Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[Facial injuries treated in the Grenoble University Hospital. Epidemiological analysis of 961 patients managed in one year].
|
INTRODUCTION: The purpose of this study was to determine the types of facial injuries treated in a one-year in a maxillo-facial unit operating in a mountainous region.METHODS: All patients admitted to the Grenoble University Hospital maxillo-facial unit for a one year period were studied. We noted cause of trauma, age, sex, type and location of fracture, type of soft tissue injury, time between trauma and surgery.RESULTS: A total of 994 patients presenting maxillo-facial trauma underwent surgery over one year; 30% of the unit's maxillo-facial surgical activity. On average, 80 patients were treated for maxillo-facial trauma per month, with a peak of 97 facial injuries in July; 65.6% were hospitalized in the maxillo-facial unit; 25,4% of the injured were aged between 21 and 30 years. Sex-ratio was 2.7M/1F. The most frequent cause was sports injuries (25.8%) followed, in decreasing order, by traffic injuries (23.1%), home injuries (17.6%), fight injuries (3.4%), work injuries (3.4%) and dog bites (3.2%). 10.5% of the injuries occurred in a mountainous setting and 40.7% were sports injuries, 95% of which during practice of winter's sports. Injuries included facial fractures (65.5%) with or without soft tissue damage, and soft tissue injuries only (34.5%); 33.6% of the patients had other lesions of the body. 67.2% underwent surgery within the first 24 hours and 86.9% before the fifth day.DISCUSSION: Sports accidents are the leading cause of facial trauma in the mountainous regions. Most facial injuries result from ski, surf and other winter sports accidents. Most of the victims were given surgical care within the first 24 hours following the accident.
|
['Accidents, Home', 'Accidents, Traffic', 'Adolescent', 'Adult', 'Age Factors', 'Aged', 'Aged, 80 and over', 'Animals', 'Athletic Injuries', 'Bites and Stings', 'Child', 'Dogs', 'Epidemiologic Studies', 'Facial Bones', 'Facial Injuries', 'Female', 'France', 'Humans', 'Male', 'Middle Aged', 'Occupational Diseases', 'Sex Factors', 'Skull Fractures', 'Soft Tissue Injuries', 'Violence']
| 16,523,173
|
[['N06.850.135.217'], ['N06.850.135.392'], ['M01.060.057'], ['M01.060.116'], ['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100'], ['M01.060.116.100.080'], ['B01.050'], ['C26.115'], ['C25.723.127', 'C26.176'], ['M01.060.406'], ['B01.050.150.900.649.313.750.250.216.200'], ['E05.318.372.500', 'N05.715.360.330.500', 'N06.850.520.450.500'], ['A02.835.232.781.324'], ['C10.900.300.284', 'C26.915.300.425'], ['Z01.542.286'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C24'], ['N05.715.350.675', 'N06.850.490.875'], ['C10.900.300.918', 'C26.404.750', 'C26.915.300.745'], ['C26.808'], ['I01.198.240.856', 'I01.880.735.900']]
|
['Health Care [N]', 'Named Groups [M]', 'Organisms [B]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]', 'Geographicals [Z]', 'Anthropology, Education, Sociology, and Social Phenomena [I]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 1
|
[Expression of estrogen sulfotransferase in breast tissues and the regulation of ESTmRNA by estrogen].
|
OBJECTIVE: To detect the expression of estrogen sulfotransferase (EST) in breast tissues, and to investigate its significance in breast carcinogenesis and the relationship between EST mRNA and estrogen.METHODS: Seven specimens of normal human breast tissues from patients with benign breast diseases were implanted subcutaneously into athymic nude mice, one specimen divided into many pieces to be implanted into 2 groups of mice, totalling 14 mice. Then the 14 mice were divided into 2 groups: as control and the other injected intramuscularly with estradiol benzoate (B-E(2)) every other day for 6 weeks. Six weeks after, the implanted tissues were taken out. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of EST mRNA in 24 specimens of breast cancer, 6 specimens of normal breast tissues around the breast fibroadenoma, all resected during operation, and the specimens of implanted normal breast tissues from the mice (control group and B-E(2) group). The protein expression of estrogen receptor (ER) was detected by immunohistochemistry in the 24 specimens of breast cancer.RESULTS: EST mRNA was expressed in all normal human breast tissues and the expression was increased in the grafts from mice of B-E(2) group. However, the specimens of breast cancer showed decrease or absence of EST mRNA expression.CONCLUSION: EST mRNA expression is decreased or lost in breast cancer tissues. EST mRNA can be regulated by estrogen in normal breast tissues.
|
['Adult', 'Breast', 'Estradiol', 'Female', 'Gene Expression Regulation, Enzymologic', 'Humans', 'Middle Aged', 'RNA, Messenger', 'Sulfotransferases']
| 14,642,074
|
[['M01.060.116'], ['A01.236'], ['D04.210.500.365.415.248', 'D06.472.334.851.437.500'], ['G05.308.320'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['D13.444.735.544'], ['D08.811.913.817.400']]
|
['Named Groups [M]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
[The glutathione peroxidase 1 (GPX1) single nucleotide polymorphism pro198Leu: association with life span and coronary artery disease].
|
In this study we genotyped polymorphism in GPX1 Pro198Leu (C > T) rs 1050450 in four groups: patients with coronary artery disease, long-livers - above 90 years, early died peoples (before 55 years) from cardiovascular diseases and Russian population as control group. We have found significant higher allele T frequency in men with coronary artery disease -34.84% (Chi2 = 5.228, p = 0.022; OR = 1.46) and in early died men from cardiovascular diseases--38.16% (Chi2 = 6.461, p = 0.011; OR = 1.69) compared with control men--26.8%. Moreover, significantly higher genotype TT frequency has been shown in patients with coronary artery disease and myocardial infarction before age 50--19.44% in comparison with control group--7.28% (Chi2 = 9.55, p = 0.002). The TT frequency in long-livers (4.39%) was the lowest and significantly different from coronary artery disease group--12.79% (Chi2 = 8.07, p = 0.0045) and from coronary artery disease subgroup with myocardial infarction before 50--19.44% (Chi2 = 14.49, p = 0.0001). Thus our results indicate that allele T (Leu) of GPX1 Pro198Leu (C > T) polymorphism is unfavorable for successful ageing. It predisposes to coronary heart disease, earlier myocardial infarction (before age 50) and earlier death (before age 55).
|
['Age Factors', 'Aged, 80 and over', 'Alleles', 'Case-Control Studies', 'Coronary Artery Disease', 'European Continental Ancestry Group', 'Female', 'Gene Frequency', 'Genetic Association Studies', 'Genetic Predisposition to Disease', 'Glutathione Peroxidase', 'Humans', 'Longevity', 'Male', 'Middle Aged', 'Myocardial Infarction', 'Polymorphism, Single Nucleotide', 'Risk Factors', 'Russia']
| 22,888,637
|
[['N05.715.350.075', 'N06.850.490.250'], ['M01.060.116.100.080'], ['G05.360.340.024.340.030'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['C14.280.647.250.260', 'C14.907.137.126.339', 'C14.907.585.250.260'], ['M01.686.508.400'], ['G05.330'], ['E05.393.385'], ['C23.550.291.687.500', 'G05.380.355'], ['D08.811.682.732.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G07.345.124.519', 'G07.540'], ['M01.060.116.630'], ['C14.280.647.500', 'C14.907.585.500', 'C23.550.513.355.750', 'C23.550.717.489.750'], ['G05.365.795.598'], ['E05.318.740.600.800.725', 'N05.715.350.200.700', 'N05.715.360.750.625.700.700', 'N06.850.490.625.750', 'N06.850.520.830.600.800.725'], ['Z01.252.122.500', 'Z01.542.248.775']]
|
['Health Care [N]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Annexin A6 and Late Endosomal Cholesterol Modulate Integrin Recycling and Cell Migration.
|
Annexins are a family of proteins that bind to phospholipids in a calcium-dependent manner. Earlier studies implicated annexin A6 (AnxA6) to inhibit secretion and participate in the organization of the extracellular matrix. We recently showed that elevated AnxA6 levels significantly reduced secretion of the extracellular matrix protein fibronectin (FN). Because FN is directly linked to the ability of cells to migrate, this prompted us to investigate the role of AnxA6 in cell migration. Up-regulation of AnxA6 in several cell models was associated with reduced cell migration in wound healing, individual cell tracking and three-dimensional migration/invasion assays. The reduced ability of AnxA6-expressing cells to migrate was associated with decreased cell surface expression of áVâ3 and á5â1 integrins, both FN receptors. Mechanistically, we found that elevated AnxA6 levels interfered with syntaxin-6 (Stx6)-dependent recycling of integrins to the cell surface. AnxA6 overexpression caused mislocalization and accumulation of Stx6 and integrins in recycling endosomes, whereas siRNA-mediated AnxA6 knockdown did not modify the trafficking of integrins. Given our recent findings that inhibition of cholesterol export from late endosomes (LEs) inhibits Stx6-dependent integrin recycling and that elevated AnxA6 levels cause LE cholesterol accumulation, we propose that AnxA6 and blockage of LE cholesterol transport are critical for endosomal function required for Stx6-mediated recycling of integrins in cell migration.
|
['Animals', 'Annexin A6', 'CHO Cells', 'Cell Line, Tumor', 'Cell Membrane', 'Cell Movement', 'Cells, Cultured', 'Cholesterol', 'Cricetulus', 'Endosomes', 'Fibroblasts', 'Humans', 'Integrin alpha5beta1', 'Integrin alphaVbeta3', 'Mice', 'Microscopy, Confocal', 'Microscopy, Video', 'Qa-SNARE Proteins', 'RNA Interference', 'Rats', 'Recombinant Proteins', 'Time-Lapse Imaging']
| 26,578,516
|
[['B01.050'], ['D12.776.157.125.050.110'], ['A11.251.210.200', 'A11.436.155'], ['A11.251.210.190', 'A11.251.860.180'], ['A11.284.149'], ['G04.198', 'G07.568.500.180'], ['A11.251'], ['D04.210.500.247.222.284', 'D04.210.500.247.808.197', 'D10.570.938.208'], ['B01.050.150.900.649.313.992.635.075.250.250'], ['A11.284.430.214.190.875.190.880.337'], ['A11.329.228'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.395.550.625.379', 'D12.776.543.550.625.379', 'D12.776.543.750.705.408.530.750', 'D12.776.543.750.705.408.850.349', 'D12.776.543.750.705.675.460', 'D12.776.543.750.705.876.374'], ['D12.776.395.550.625.439', 'D12.776.543.550.625.439', 'D12.776.543.750.705.408.460.870.500', 'D12.776.543.750.705.675.541'], ['B01.050.150.900.649.313.992.635.505.500'], ['E01.370.350.515.395', 'E05.595.395'], ['E01.370.350.515.690', 'E05.595.690', 'J01.897.280.500.898.475', 'L01.178.820.090.898.475', 'L01.178.847.823.475'], ['D12.776.543.512.249.500.500', 'D12.776.543.990.775.500.500'], ['G05.308.203.374.790'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.828'], ['E01.370.350.600.817', 'E05.712.657', 'L01.280.960.399']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Technology, Industry, and Agriculture [J]', 'Information Science [L]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
|
Motor sensory dysfunction of upper limb due to conversion syndrome.
|
Seven patients had functional paralysis that occurred in the dominant limb together with joint contracture and sensory disturbances associated with emotional problems. In five of these patients, the syndrome was preceded by trauma to the affected upper limb and in one patient by a myocardial infarction. The treatment consisted of persuasion, suggestion, general rhythmic exercises and emotional support given by the physiatrist (not a psychiatrist). In five of the subjects treated, the symptoms disappeared and the patients soon returned to work. In two patients the treatment did not succeed since no satisfactory rapport could be established between the patient and the physician.
|
['Adolescent', 'Adult', 'Arm', 'Child', 'Conversion Disorder', 'Female', 'Humans', 'Male', 'Middle Aged', 'Paralysis']
| 1,247,371
|
[['M01.060.057'], ['M01.060.116'], ['A01.378.800.075'], ['M01.060.406'], ['F03.875.300'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['C10.597.622', 'C23.888.592.636']]
|
['Named Groups [M]', 'Anatomy [A]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Diseases [C]']
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Evolving residency requirements for ambulatory care training for five medical specialties, 1961 to 1989.
|
Recent changes in the patient population of teaching hospitals, spurred by technologic advances and economic forces, have jeopardized the traditional hospital-based model of residency training. In consequence, there has been increasing attention paid to the need for ambulatory care experience. A primary force in shaping the content of postgraduate medical education is "The Essentials of Accredited Residencies," published in the Directory of Graduate Medical Education Programs. We reviewed recommendations and requirements for ambulatory settings and outpatient experience as specified in the Directory during the years 1961 to 1988 and investigated pending changes in requirements for five major specialties: internal medicine, pediatrics, family practice, general surgery, and obstetrics and gynecology. Increases in the amount of time residents spend in ambulatory care training recently have been mandated in internal medicine and are under consideration in two other specialties, indicating probable major shifts in the locus of postgraduate medical training.
|
['Ambulatory Care', 'Curriculum', 'Education, Medical', 'Education, Medical, Graduate', 'Humans', 'Internship and Residency', 'Specialization']
| 2,618,049
|
[['E02.760.106', 'N02.421.585.106'], ['I02.158'], ['I02.358.399'], ['I02.358.337.350', 'I02.358.399.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I02.358.337.350.500', 'I02.358.399.350.750'], ['H02.811']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 1
| 0
|
Anterior cruciate ligament reconstruction in men and women: An outcome analysis comparing gender.
|
PURPOSE: Recent studies have shown that female athletes suffer a higher incidence of anterior cruciate ligament (ACL) tears than comparable male athletes. The purpose of this study was to evaluate the effect gender has on outcome in ACL reconstruction using bone-patellar tendon-bone autograft.TYPE OF STUDY: Retrospective case review and outcome study.METHODS: A retrospective review of a single surgeon's practice revealed 279 ACL reconstructions that met our criteria for inclusion. Two-hundred forty-nine of these patients (91%) were contacted. Two-hundred (72%) were evaluated with physical examination, KT-1000 testing, functional testing, and radiographic evaluation. Outcome was assessed with Tegner, Lysholm, modified HSS, and Cincinnati Knee rating scales, as well as the SF-36 health survey and a self-administered questionnaire. There were 137 men and 63 women. Data were evaluated with Wilcoxon rank sum testing, analysis of variance testing, chi-square analysis, and the Student t test. The level of significance was set at P <.05.RESULTS: Postoperatively, no differences were noted on Lachman, anterior drawer, pivot shift, or functional testing in either groups. Male patients had a significantly greater mean prone heel height difference (1.80 v 1.10 cm, P =.0018) and mean KT-1000 maximum manual side-to-side difference (0.76 v 1.73 mm, P =.014). However, no differences were noted in the percentage of patients with greater than 5-mm side-to-side difference, with 5 men (4%) and 2 women (3%) classified as arthrometric failures. No differences were noted in mean Tegner, Lysholm, Noyes Cincinnati, and modified HSS scores. Men had significantly lower HSS radiographic scores (24.98 v 26.22, P =.0016). Men and women were compared with gender-matched controls for SF-36 testing, and women scored higher compared with controls than did men in the Role Physical, Body Pain, and General Health categories. No differences were noted in either group regarding donor-site pain, patellofemoral crepitance, or problems with stair climbing. Ninety-six percent of men and 98% of women would have had the surgery over again given similar circumstances.CONCLUSIONS: Objective criteria failed to detect clinically significant differences in physical examination and arthrometric results between men and women. Knee rating scale scores were similar. Comparable outcome with high satisfaction and equal success can be expected in both men and women undergoing ACL reconstruction using bone-patellar tendon-bone autograft. No basis exists for the inclusion of gender as a determining factor regarding the decision to perform ACL reconstructive surgery with bone-patellar tendon-bone autograft.
|
['Activities of Daily Living', 'Adolescent', 'Adult', 'Anterior Cruciate Ligament', 'Anterior Cruciate Ligament Injuries', 'Athletic Injuries', 'Basketball', 'Bone Screws', 'Female', 'Follow-Up Studies', 'Humans', 'Knee Joint', 'Length of Stay', 'Male', 'Menisci, Tibial', 'Middle Aged', 'Patella', 'Patient Satisfaction', 'Physical Examination', 'Radiography', 'Retrospective Studies', 'Sex Distribution', 'Sex Factors', 'Soccer', 'Tendons', 'Tibia', 'Treatment Outcome']
| 11,447,545
|
[['E02.760.169.063.500.067', 'E02.831.067', 'I03.050', 'N02.421.784.110'], ['M01.060.057'], ['M01.060.116'], ['A02.513.514.100', 'A02.835.583.512.100', 'A10.165.669.514.100'], ['C26.558.554.213'], ['C26.115'], ['I03.450.642.845.117'], ['E07.695.370.437', 'E07.858.442.660.460.437', 'E07.858.690.725.460.437'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.583.475'], ['E02.760.400.480', 'N02.421.585.400.480'], ['A02.165.308.538.500', 'A02.835.583.475.590', 'A10.165.382.350.163.500'], ['M01.060.116.630'], ['A02.835.232.043.650.624', 'A02.835.232.730.500'], ['F01.100.150.750.625', 'F01.145.488.887.625', 'N04.452.822.700', 'N05.300.150.800.625', 'N05.715.360.600'], ['E01.370.600'], ['E01.370.350.700'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['I01.240.800', 'N01.224.803', 'N06.850.505.400.850'], ['N05.715.350.675', 'N06.850.490.875'], ['I03.450.642.845.800'], ['A02.880'], ['A02.835.232.043.650.883'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]', 'Anatomy [A]', 'Diseases [C]', 'Organisms [B]', 'Psychiatry and Psychology [F]']
| 1
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Reactive and clonal thrombocytosis: proinflammatory and hematopoietic cytokines and acute phase proteins.
|
BACKGROUND: We quantitated proinflammatory and thrombopoietic cytokines in reactive thrombocytosis (RT) and clonal thrombocytosis (CT) to identify a cytokine profile that might aid in the distinction of these two disorders.METHODS: Serum levels of cytokines relevant to platelet biology--interleukins 3, 6, 11, and 1beta; thrombopoietin; tumor necrosis factor alpha; and C-reactive protein (CRP)--were measured by enzyme-linked immunosorbent assay in healthy subjects and in patients with CT and RT.RESULTS: Interleukin-6 and CRP levels were higher in RT patients than in controls or CT patients. Interleukin 1beta levels were higher in the RT group than in the CT and control groups.CONCLUSIONS: In RT, IL-6, IL-1beta, and CRP levels are elevated. In both RT and CT, IL-11 is elevated, but thrombopoietin levels are not.
|
['Adult', 'Aged', 'Analysis of Variance', 'C-Reactive Protein', 'Case-Control Studies', 'Diagnosis, Differential', 'Enzyme-Linked Immunosorbent Assay', 'Female', 'Humans', 'Interleukin-1', 'Interleukin-11', 'Interleukin-3', 'Interleukin-6', 'Male', 'Middle Aged', 'Platelet Count', 'Sensitivity and Specificity', 'Thrombocytosis', 'Thrombopoietin', 'Tumor Necrosis Factor-alpha']
| 11,332,909
|
[['M01.060.116'], ['M01.060.116.100'], ['E05.318.740.150', 'N05.715.360.750.125', 'N06.850.520.830.150'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['E01.171'], ['E05.478.566.350.170', 'E05.478.566.380.360', 'E05.478.583.400.170', 'E05.601.470.350.170', 'E05.601.470.380.360'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.644.276.374.465.010', 'D12.644.276.374.500.400', 'D12.776.467.374.465.010', 'D12.776.467.374.500.400', 'D23.529.374.465.131', 'D23.529.374.500.400'], ['D12.644.276.374.465.511', 'D12.776.467.374.465.511', 'D23.529.374.465.511'], ['D12.644.276.374.410.240.400', 'D12.644.276.374.465.032', 'D12.776.395.240.400', 'D12.776.467.374.410.240.400', 'D12.776.467.374.465.032', 'D23.529.374.410.240.400', 'D23.529.374.465.169'], ['D12.644.276.374.465.224', 'D12.776.467.374.465.202', 'D23.529.374.465.224'], ['M01.060.116.630'], ['E01.370.225.500.195.107.740', 'E01.370.225.625.107.700', 'E01.370.225.625.625.625', 'E05.200.500.195.107.740', 'E05.200.625.107.700', 'E05.200.625.625.625', 'E05.242.195.107.740', 'G04.140.107.740', 'G09.188.105.700'], ['E05.318.370.800', 'E05.318.740.872', 'G17.800', 'N05.715.360.325.700', 'N05.715.360.750.725', 'N06.850.520.445.800', 'N06.850.520.830.872'], ['C15.378.140.860', 'C15.378.190.636.860'], ['D12.644.276.374.410.240.750', 'D12.776.395.240.750', 'D12.776.467.374.410.240.750', 'D23.529.374.410.240.750'], ['D12.644.276.374.500.800', 'D12.644.276.374.750.626', 'D12.776.124.900', 'D12.776.395.930', 'D12.776.467.374.500.800', 'D12.776.467.374.750.626', 'D23.529.374.500.800', 'D23.529.374.750.626']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Density and diameter of dentinal tubules in etched and non-etched bovine dentine examined by scanning electron microscopy.
|
Bovine teeth have been widely used in studies focusing adhesion to dentine over the last years. However, little is known about main structural aspects of bovine dentine, especially regarding density and diameter of its tubules. Thirty bovine incisors were randomly divided into two groups. In group I, teeth were cross-sectioned at three depths: outer, middle and inner. The dentinal surfaces were etched with 35% phosphoric acid for 90s. In group II, the teeth were fractured at the same three depths. All the specimens were processed and examined in a Jeol 6100 SEM. Fifteen micrographs from each depth were obtained for the two groups at a magnification of 2500x. To determine the diameter of the dentinal tubules, the LEICA Q500 MC software was used. All the results were statistically processed in the EXCEL software. The dentinal tubules were wider at the outer regions (GI: 5.21+/-0.64microm; GII: 2.30+/-0.09microm) than that at the inner regions (GI: 2.71+/-0.72microm; GII: 1.77+/-0.06microm). Tubular density was higher at the inner regions (50310+/-11178tubules/mm(2)) than that at the outer regions (18772+/-2951tubules/mm(2)). In addition, the peritubular dentine was wider at the outer depth than at the inner one. Our results showed that bovine dentine possesses some structural aspects different from those previously reported for human dentine, mainly related to the diameter of dentinal tubules and to the thickness of peritubular dentine at the several depths.
|
['Acid Etching, Dental', 'Animals', 'Cattle', 'Dentin', 'Image Processing, Computer-Assisted', 'Microscopy, Electron, Scanning', 'Models, Animal', 'Phosphoric Acids', 'Random Allocation', 'Stress, Mechanical', 'Time Factors', 'Tooth Fractures']
| 17,433,249
|
[['E06.931.475.111'], ['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['A14.549.167.900.280'], ['L01.224.308'], ['E01.370.350.515.402.541', 'E05.595.402.541'], ['E05.598'], ['D01.029.260.700.675', 'D01.695.625.675'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['G01.374.835'], ['G01.910.857'], ['C07.793.850.750', 'C26.900.750']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Anatomy [A]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 1
| 0
|
[Clinical analysis of 108 cases of jaw ameloblastoma].
|
PURPOSE: To review the age of onset, gender, sites, pathologic types, operation methods and prognosis, and explore the internal relations in 108 cases of ameloblastoma.METHODS: The patients were divided into 7 groups according to the age, and then the proportion, primary site, pathologic type, and recurrence rate of ameloblastoma in all groups were analyzed. The relationship among pathologic type, recurrence rate and primary site, as well as the relationship between operation methods and recurrence rate were evaluated.RESULTS: In 108 patients, 61 patients were 20-39 years old (56.48%) with more men than women. 88 patients occurred in the mandible(81.48%). Follicular type accounted for 45.37%, and plexiform type accounted for 62.96%. The average recurrence rate of 108 patients was 32.41%, and recurrence was more often in patients over 50 years old. The postoperative recurrence rate of 65 patients treated by curettage was 43.08%, while it was 16.28% in 43 patients treated by radical correction. The recurrent rate was 34.69% in follicular type, 26.47% in plexiform type, and 28.57% in acanthoma type and granular cell type respectively.CONCLUSIONS: Ameloblastoma usually occurs in young men, more frequently in mandibular molar region, ramus and mandibular angle. Follicular type and plexiform type are the most common pathological types. Follicular type relapses more easily. Compared with curettage, radical surgery can reduce the recurrent rate.
|
['Adult', 'Age of Onset', 'Ameloblastoma', 'Female', 'Humans', 'Male', 'Mandible', 'Mandibular Neoplasms', 'Molar', 'Neoplasm Recurrence, Local', 'Prognosis', 'Young Adult']
| 25,338,806
|
[['M01.060.116'], ['N05.715.350.075.100', 'N06.850.490.250.100'], ['C04.557.695.065'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['C04.588.149.721.450.583', 'C05.116.231.754.450.583', 'C05.500.499.583', 'C05.500.607.442', 'C07.320.515.583', 'C07.320.610.583'], ['A14.549.167.860.525'], ['C04.697.655', 'C23.550.727.655'], ['E01.789'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Health Care [N]', 'Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Increased surface expression of CD11b/CD18 (Mac-1) is not required for stimulated neutrophil adherence to cultured endothelium.
|
The mechanism whereby the human neutrophil membrane heterodimer, CD11b/CD18 (Mac-1, Mo1), mediates neutrophil adherence is not known. We studied the role of CD11b/CD18 surface expression in the promotion of neutrophil adhesiveness. We found that phorbol myristate acetate (PMA), calcium ionophore (A23187), and FMLP caused a three- to sevenfold increase in surface expression of both CD11b (alpha M) and CD18 (beta) as assayed by binding of MAbs 60.1 (anti-CD11b) and 60.3 (anti-CD18). Increased binding of MAbs was temporally associated with the promotion of neutrophil aggregation and adherence to cultured endothelial monolayers. Pretreatment of neutrophils with the anion channel-blocking agent, DIDS (4,4'-diisothiocyanostilbene-2,2'-disulfonic acid), inhibited the increased surface expression of CD11b and CD18 after stimulation by PMA, A23187, or FMLP and resulted in nearly complete inhibition of neutrophil aggregation. However, pretreatment with DIDS did not diminish either PMA-, A23187-, or FMLP-stimulated neutrophil adherence to endothelial monolayers. We also observed that stimulation of granule-depleted neutrophil cytoplasts by PMA, A23187, or FMLP induced aggregation and adherence to endothelial monolayers without increasing surface expression of CD11b or CD18. We conclude that the increased surface expression of CD11b/CD18 that occurs after stimulation is neither sufficient nor necessary for enhanced adherence to endothelium. Moreover, though both are CD11b/CD18-dependent, the mechanisms involved in neutrophil aggregation are different from those involved in neutrophil adherence to endothelium.
|
["4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid", "4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid", 'Animals', 'Antigens, Surface', 'Binding Sites, Antibody', 'Binding, Competitive', 'Cattle', 'Cell Adhesion', 'Cell Aggregation', 'Cytoplasm', 'Endothelium, Vascular', 'Humans', 'Lymphocyte Function-Associated Antigen-1', 'Neutrophils']
| 3,278,004
|
[['D02.455.426.559.389.150.700.200', 'D02.500.375.125', 'D02.886.250.125'], ['D02.455.426.559.389.150.700.050', 'D02.500.375.050', 'D02.886.250.050'], ['B01.050'], ['D23.050.301'], ['G02.111.570.060.425.079', 'G02.111.570.120.408', 'G12.122.232', 'G12.125'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['B01.050.150.900.649.313.500.380.271'], ['G04.022'], ['G04.198.251'], ['A11.284.430.214'], ['A07.015.700.500', 'A10.272.491.355'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['D12.776.395.550.200.074.937', 'D12.776.395.550.200.625.550', 'D12.776.543.550.200.093.937', 'D12.776.543.550.200.625.550', 'D12.776.543.750.705.408.600.400', 'D12.776.543.750.705.877.550', 'D23.050.301.350.074.400', 'D23.050.301.350.625.550'], ['A11.118.637.415.583', 'A11.627.340.583', 'A11.733.689', 'A15.145.229.637.415.583', 'A15.382.490.315.583', 'A15.382.680.689']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
[Long-term outcomes of children treated with continuous renal replacement therapy].
|
INTRODUCTION: The objective of this study is to analyze long-term outcomes and kidney function in children requiring continuous renal replacement therapy (CRRT) after an acute kidney injury episode.PATIENTS AND METHODS: A retrospective observational study was performed using a prospective database of 128 patients who required CRRT admitted to the pediatric intensive care unit between years 2006 and 2012. The subsequent outcomes were assessed in those surviving at hospital discharge.RESULTS: Of the 128 children who required RRT in the pediatric intensive care unit, 71 survived at hospital discharge (54.4%), of whom 66 (92.9%) were followed up. Three patients had chronic renal failure prior to admission to the NICU. Of the 63 remaining patients, 6 had prolonged or relapses of renal function disturbances, but only one patient with atypical Hemolytic Uremic Syndrome developed end-stage renal failure. The rest had normal kidney function at the last check-up.CONCLUSIONS: Most of surviving children that required CRRT have a positive outcome later on, presenting low mortality rates and recovery of kidney function in the medium term.
|
['Acute Kidney Injury', 'Child', 'Fluid Therapy', 'Follow-Up Studies', 'Humans', 'Intensive Care Units', 'Prospective Studies', 'Renal Replacement Therapy', 'Retrospective Studies', 'Treatment Outcome']
| 25,683,273
|
[['C12.777.419.780.050', 'C13.351.968.419.780.050'], ['M01.060.406'], ['E02.319.360'], ['E05.318.372.500.750.249', 'N05.715.360.330.500.750.350', 'N06.850.520.450.500.750.350'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['N02.278.388.493'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E02.870'], ['E05.318.372.500.500.500', 'E05.318.372.500.750.750', 'N05.715.360.330.500.500.500', 'N05.715.360.330.500.750.825', 'N06.850.520.450.500.500.500', 'N06.850.520.450.500.750.825'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Diseases [C]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Organisms [B]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
[SECOND STAGE IN MINIINVASIVE FETAL SURGERY FOR SEVERE CONGENITAL DIAPHRAGMATIC HERNIA. CASE REPORT].
|
We present a case of miniinvasive fetal surgery for CDH treated at 28 and 34 weeks of gestation. The first step was successfully performed at 28 weeks with Fetal Endoscopic Tracheal Occlusion with ballon. The second step was performed at 34 weeks for balloon removal. The necessity of fetal cytogenetic assessment and array CGH was carried out to exclude gene disorders that could lead to poor long-term outcome. A planned SC and optimal neonatology management were followed by a surgical operation of the newborn. Experienced interdisciplinary team successfully provide a perinatal and postnatal surgery for severe CDH. The newborn was discharged from the hospital 3 weeks after the repairing operation in a good condition.
|
['Female', 'Fetal Diseases', 'Fetoscopy', 'Hernias, Diaphragmatic, Congenital', 'Humans', 'Infant, Newborn', 'Pregnancy', 'Trachea', 'Ultrasonography, Prenatal']
| 26,411,195
|
[['C13.703.277', 'C16.300'], ['E01.370.378.630.300', 'E01.370.388.250.280', 'E02.467.750', 'E04.502.250.280', 'E04.520.280'], ['C16.131.433', 'C23.300.707.960.500.116'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703.520'], ['G08.686.784.769'], ['A04.889'], ['E01.370.350.850.865', 'E01.370.378.630.865']]
|
['Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Reconstruction of mandible with free osteocutaneous flap using deep circumflex iliac vessels as the stem.
|
One of the 2 patients introduced here had osteoradionecrosis, which has been difficult to reconstruct. The other had a massive adamantinoma that required replacement of the mandible from the midportion of the right ramus to the left angle with the iliac bone using the conventional method. The grafted iliac bone was severely absorbed, however. The mandible in both cases was successfully reconstructed with an osteocutaneous compound flap, using the deep circumflex iliac vessels as the stem. This method is considered a very good procedure for reconstruction of the mandible, which has been difficult by conventional procedures. Because the diameter of the stem is large, vascular anastomosis can be readily performed with an extremely high degree of safety. Pulsation of the stem can be palpated on follow-up even one year after surgery, and there are no signs of bone reabsorption.
|
['Ameloblastoma', 'Humans', 'Iliac Artery', 'Iliac Vein', 'Male', 'Mandible', 'Mandibular Diseases', 'Mandibular Neoplasms', 'Middle Aged', 'Osteoradionecrosis', 'Surgical Flaps']
| 7,247,250
|
[['C04.557.695.065'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A07.015.114.444'], ['A07.015.908.427'], ['A02.835.232.781.324.502.632', 'A14.521.632'], ['C05.500.607', 'C07.320.610'], ['C04.588.149.721.450.583', 'C05.116.231.754.450.583', 'C05.500.499.583', 'C05.500.607.442', 'C07.320.515.583', 'C07.320.610.583'], ['M01.060.116.630'], ['C26.733.579', 'G01.750.748.500.579'], ['A10.850.710', 'E07.862.710']]
|
['Diseases [C]', 'Organisms [B]', 'Anatomy [A]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
The stem cell/cancer stem cell marker ALDH1A3 regulates the expression of the survival factor tissue transglutaminase, in mesenchymal glioma stem cells.
|
Tissue transglutaminase (tTG), a dual-function enzyme with GTP-binding and acyltransferase activities, has been implicated in the survival and chemotherapy resistance of aggressive cancer cells and cancer stem cells, including glioma stem cells (GSCs). Using a model system comprising two distinct subtypes of GSCs referred to as proneural (PN) and mesenchymal (MES), we find that the phenotypically aggressive and radiation therapy-resistant MES GSCs exclusively express tTG relative to PN GSCs. As such, the self-renewal, proliferation, and survival of these cells was sensitive to treatment with tTG inhibitors, with a benefit being observed when combined with the standard of care for high grade gliomas (i.e. radiation or temozolomide). Efforts to understand the molecular drivers of tTG expression in MES GSCs revealed an unexpected link between tTG and a common marker for stem cells and cancer stem cells, Aldehyde dehydrogenase 1A3 (ALDH1A3). ALDH1A3, as well as other members of the ALDH1 subfamily, can function in cells as a retinaldehyde dehydrogenase to generate retinoic acid (RA) from retinal. We show that the enzymatic activity of ALDH1A3 and its product, RA, are necessary for the observed expression of tTG in MES GSCs. Additionally, the ectopic expression of ALDH1A3 in PN GSCs is sufficient to induce the expression of tTG in these cells, further demonstrating a causal link between ALDH1A3 and tTG. Together, these findings ascribe a novel function for ALDH1A3 in an aggressive GSC phenotype via the up-regulation of tTG, and suggest the potential for a similar role by ALDH1 family members across cancer types.
|
['Aldehyde Oxidoreductases', 'Biomarkers, Tumor', 'Brain Neoplasms', 'Cell Line, Tumor', 'Cell Proliferation', 'Cell Survival', 'Dacarbazine', 'GTP-Binding Proteins', 'Gene Expression Regulation, Neoplastic', 'Glioma', 'Humans', 'Mesenchymal Stem Cells', 'Neoplastic Stem Cells', 'RNA, Small Interfering', 'Stem Cells', 'Temozolomide', 'Transglutaminases', 'Tretinoin', 'Up-Regulation']
| 28,423,611
|
[['D08.811.682.657.163'], ['D23.101.140'], ['C04.588.614.250.195', 'C10.228.140.211', 'C10.551.240.250'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.161.750', 'G07.345.249.410.750'], ['G04.346'], ['D02.925.200', 'D03.383.129.308.240'], ['D08.811.277.040.330.300', 'D12.776.157.325'], ['G05.308.370'], ['C04.557.465.625.600.380', 'C04.557.470.670.380', 'C04.557.580.625.600.380'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['A11.329.830.500', 'A11.872.590.500'], ['A11.872.650'], ['D13.150.650.700', 'D13.444.735.150.700', 'D13.444.735.790.552.875'], ['A11.872'], ['D02.925.200.500', 'D03.383.129.308.240.500'], ['D08.811.913.050.200.800'], ['D02.455.326.271.665.202.495.818.500', 'D02.455.426.392.368.367.379.249.700.860.500', 'D02.455.849.131.495.818.800', 'D02.455.849.291.925.500', 'D23.767.261.700.780'], ['G02.111.905', 'G05.308.850', 'G07.690.773.998']]
|
['Chemicals and Drugs [D]', 'Diseases [C]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Video-assisted tricuspid valve surgery: a new surgical option in endocarditis on pacemaker.
|
A patient presenting with a pacemaker lead infection and tricuspid regurgitation underwent a minimally invasive video-assisted tricuspid valve replacement. The valve was approached through a right anterior mini thoracotomy. Under thoracoscopic vision and peripheral cardiopulmonary bypass, a catheter was placed on the ascending aorta for antegrade cardioplegia delivery. A transthoracic aortic cross-clamp was introduced through the third right intercostal space. Tricuspid valve replacement added to the pacemaker leads ablation was exclusively performed under thoracoscopic vision, providing an excellent video-image in this reduced operative field. After 22 months of follow up, the patient is asymptomatic, the echocardiography showing a normally functioning valve.
|
['Cardiopulmonary Bypass', 'Echocardiography, Transesophageal', 'Endocarditis, Bacterial', 'Heart Arrest, Induced', 'Heart Valve Prosthesis Implantation', 'Humans', 'Male', 'Middle Aged', 'Minimally Invasive Surgical Procedures', 'Pacemaker, Artificial', 'Prosthesis Failure', 'Prosthesis-Related Infections', 'Staphylococcal Infections', 'Staphylococcus', 'Tricuspid Valve', 'Tricuspid Valve Insufficiency', 'Video Recording']
| 10,485,429
|
[['E04.292.413'], ['E01.370.350.130.750.235', 'E01.370.350.850.220.235', 'E01.370.370.380.220.235'], ['C01.150.252.245', 'C01.190.249', 'C14.260.249', 'C14.280.282.407'], ['E04.100.376.374', 'E04.928.220.360'], ['E04.100.376.485', 'E04.650.410', 'E04.928.220.410'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['E04.502'], ['E07.305.250.750'], ['C23.550.767.865', 'E05.325.771'], ['C01.685', 'C23.550.767.868'], ['C01.150.252.410.868'], ['B03.300.390.400.800.750', 'B03.353.500.750.750', 'B03.510.100.750.750', 'B03.510.400.790.750'], ['A07.541.510.893'], ['C14.280.484.856'], ['L01.280.960']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Anatomy [A]', 'Information Science [L]']
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
| 0
|
Complete nucleotide sequence of pLD-TEX-KL, a 66-kb plasmid of Legionella dumoffii TEX-KL strain.
|
The complete nucleotide sequence of a large (66 kb) plasmid pLD-TEX-KL of Legionella dumoffii TEX-KL strain was determined. Of the 57 predicted open reading frames (ORFs), 39 (68%) encoded proteins similar to previously known proteins, five (9%) were assigned with putative functions, three (5%) encoded conserved hypothetical proteins, and 10 (18%) had no homology to any genes present in the current open databases. The ORFs with similar functions were organized in a modular structure; thus, transfer region was identified, as well as a putative heavy-metal ion transporter system (hel). The transfer region encoded homologs of the Salmonella entrica serovar Typhi conjugative system components involved in conjugation. In addition, we also found a potential protein that was analogous to the DNA polymerase III epsilon subunit. It is rarely found that plasmid encode the DNA polymerase.
|
['Amino Acid Sequence', 'Bacterial Proteins', 'Base Sequence', 'Carrier Proteins', 'Conjugation, Genetic', 'DNA Polymerase III', 'DNA, Bacterial', 'Legionella', 'Molecular Sequence Data', 'Open Reading Frames', 'Phylogeny', 'Plasmids']
| 17,881,053
|
[['G02.111.570.060', 'L01.453.245.667.060'], ['D12.776.097'], ['G02.111.570.080', 'G05.360.080', 'L01.453.245.667.080'], ['D12.776.157'], ['G05.728.200'], ['D08.811.913.696.445.308.300.235'], ['D13.444.308.212'], ['B03.440.400.425.450.450', 'B03.660.250.460.460'], ['L01.453.245.667'], ['G05.360.335.760.640', 'G05.360.340.024.340.137.650'], ['G05.697', 'G16.075.605', 'L01.100.697'], ['G05.360.600']]
|
['Phenomena and Processes [G]', 'Information Science [L]', 'Chemicals and Drugs [D]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Echinococcosis (hydatid disease) in Missouri: diagnosis by fine-needle aspiration of a lung cyst.
|
Echinococcus granulosus was diagnosed by fine-needle aspiration cytology of a lung cyst in a 6-yr-old white female in central Missouri. No adverse reaction occurred following the aspiration. The cytologic sample yielded clear fluid containing numerous clearly identifiable protoscoleces diagnostic for echinococcosis using routine PAP staining. Since hydatid disease is extremely uncommon in the Midwest, it had not initially been considered in the differential diagnosis. The infection was probably not indigenous to Missouri, since the patient lived the first 3 1/2 yr of life in Alaska, where the organism is endemic. This can only be speculative, however, since echinococcal organisms are found in wildlife in the Midwest.
|
['Biopsy, Needle', 'Child', 'Echinococcosis, Pulmonary', 'Female', 'Humans', 'Missouri']
| 1,954,835
|
[['E01.370.225.500.384.100.119', 'E01.370.225.998.054.119', 'E01.370.388.100.100', 'E04.074.119', 'E04.665.100', 'E05.200.500.384.100.119', 'E05.200.998.054.119', 'E05.242.384.100.119'], ['M01.060.406'], ['C01.610.335.190.396.480', 'C01.610.582.314', 'C01.748.450.314', 'C08.381.517.314', 'C08.730.450.314'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['Z01.107.567.875.510.515']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Named Groups [M]', 'Diseases [C]', 'Organisms [B]', 'Geographicals [Z]']
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 1
|
Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias.
|
Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1st and the 2nd trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3rd trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.
|
['Adult', 'Cohort Studies', 'Erythrocytes', 'Female', 'Glutathione Peroxidase', 'Humans', 'Oxidation-Reduction', 'Oxidoreductases', 'Pregnancy', 'Pregnancy Complications, Hematologic', 'Thrombophilia']
| 32,555,583
|
[['M01.060.116'], ['E05.318.372.500.750', 'N05.715.360.330.500.750', 'N06.850.520.450.500.750'], ['A11.118.290', 'A11.443.240', 'A15.145.229.334'], ['D08.811.682.732.500'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.700', 'G03.295.531'], ['D08.811.682'], ['G08.686.784.769'], ['C13.703.667', 'C15.378.785'], ['C15.378.925']]
|
['Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Diseases [C]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Quality of life in patients 2 to 4 years after closed head injury.
|
This study evaluated quality of life in 78 patients with closed head injury (CHI) 2 to 4 years postinjury. Using both interview data and mean data from the Sickness Impact Profile questionnaire, impaired quality of life was observed in the areas of psychosocial functioning, social role functioning, leisure activities, and, to a lesser extent, physical functioning, during chronic phases of recovery. Relatives and close friends reported by means of the Katz Adjustment Scale that the CHI patients showed a series of negative behavioral symptoms 2 to 4 years postinjury. These data suggest that CHI patients may experience impaired quality of life in a number of domains well beyond the acute postinjury phases. An attempt was also made to compare patients' and relatives' reports of patient quality of life. Preliminary analyses indicated modest correspondence between relatives' and patients' ratings of some areas of postinjury dysfunction, including cognitive and behavioral slowing and social withdrawal.
|
['Activities of Daily Living', 'Adolescent', 'Adult', 'Craniocerebral Trauma', 'Emotions', 'Female', 'Humans', 'Male', 'Quality of Life', 'Self Care', 'Social Behavior']
| 3,785,620
|
[['E02.760.169.063.500.067', 'E02.831.067', 'I03.050', 'N02.421.784.110'], ['M01.060.057'], ['M01.060.116'], ['C10.900.300', 'C26.915.300'], ['F01.470'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['I01.800', 'K01.752.400.750', 'N06.850.505.400.425.837'], ['E02.900', 'I03.050.563', 'N02.421.784.680'], ['F01.145.813']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Humanities [K]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 1
| 0
|
Gametocyte clearance dynamics following oral artesunate treatment of uncomplicated falciparum malaria in Malian children.
|
Artemisinin-based combination therapies decrease Plasmodium gametocyte carriage. However, the role of artesunate in monotherapy in vivo, the mechanisms involved, and the utility of gametocyte carriage as a potential tool for the surveillance of antimalarial resistance are poorly understood. In 2010-2011, we conducted an open-label, prospective efficacy study of artesunate as monotherapy in children 1-10 years of age with uncomplicated falciparum malaria in Bougoula-Hameau, Mali. Standard oral doses of artesunate were administered for 7 days and patients were followed up for 28 days. The data were compared to a similar study conducted in 2002-2004. Of 100 children enrolled in the 2010-2011 study, 92 were analyzed and compared to 217 children enrolled in the 2002-2004 study. The proportion of gametocyte carriers was unchanged at the end of treatment (23% at baseline vs. 24% on day 7, p = 1.0) and did not significantly decline until day 21 of follow-up (23% vs. 6%, p = 0.003). The mean gametocyte density at inclusion remained unchanged at the end of treatment (12 gametocytes/ìL vs. 16 gametocytes/ìL, p = 0.6). Overall, 46% of the 71 initial non-carriers had gametocytes detected by day 7. Similar results were found in the 2002-2004 study. In both studies, although gametocyte carriage significantly decreased by the end of the 28-day follow-up, artesunate did not clear mature gametocytes during treatment and did not prevent the appearance of new stage V gametocytes as assessed by light microscopy. Baseline gametocyte carriage was significantly higher 6 years after the deployment of artemisinin-based combination therapies in this setting.
|
['Animals', 'Antimalarials', 'Artemisinins', 'Artesunate', 'Carrier State', 'Child', 'Child, Preschool', 'Drug Resistance', 'Female', 'Germ Cells', 'Humans', 'Infant', 'Malaria, Falciparum', 'Male', 'Mali', 'Parasitemia', 'Plasmodium falciparum', 'Prospective Studies', 'Single-Blind Method']
| 26,839,003
|
[['B01.050'], ['D27.505.954.122.250.100.085'], ['D01.248.497.158.685.750.212', 'D01.339.431.374.212', 'D01.650.550.750.200', 'D02.389.338.055', 'D02.455.849.765.211'], ['D01.248.497.158.685.750.212.500', 'D01.339.431.374.212.500', 'D01.650.550.750.200.500', 'D02.389.338.055.500', 'D02.455.849.765.211.500'], ['N06.850.520.169'], ['M01.060.406'], ['M01.060.406.448'], ['G07.690.773.984'], ['A05.360.490', 'A11.497'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.703'], ['C01.610.752.530.650', 'C01.920.875.650'], ['Z01.058.290.190.500'], ['C01.610.695', 'C23.550.470.790.500.580'], ['B01.043.075.380.611.561'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['E05.318.370.850', 'N05.715.360.325.730', 'N06.850.520.445.850']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Named Groups [M]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Diseases [C]', 'Geographicals [Z]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 1
|
Morphological changes and nuclear translocation of DLC1 tumor suppressor protein precede apoptosis in human non-small cell lung carcinoma cells.
|
We have previously shown that reactivation of DLC1, a RhoGAP containing tumor suppressor gene, inhibits tumorigenicity of human non-small cell lung carcinoma cells (NSCLC). After transfection of NSCLC cells with wild type (WT) DLC1, changes in cell morphology were observed. To determine whether such changes have functional implications, we generated several DLC1 mutants and examined their effects on cell morphology, proliferation, migration and apoptosis in a DLC1 deficient NSCLC cell line. We show that WT DLC1 caused actin cytoskeleton-based morphological alterations manifested as cytoplasmic extensions and membrane blebbings in most cells. Subsequently, a fraction of cells exhibiting DLC1 protein nuclear translocation (PNT) underwent caspase 3-dependent apoptosis. We also show that the RhoGAP domain is essential for the occurrence of morphological alterations, PNT and apoptosis, and the inhibition of cell migration. DLC1 PNT is dependent on a bipartite nuclear localizing sequence and most likely is regulated by a serine-rich domain at N-terminal part of the DLC1 protein. Also, we found that DLC1 functions in the cytoplasm as an inhibitor of tumor cell proliferation and migration, but in the nucleus as an inducer of apoptosis. Our analyses provide evidence for a possible link between morphological alterations, PNT and proapoptotic and anti-oncogenic activities of DLC1 in lung cancer.
|
['Actins', 'Amino Acid Sequence', 'Apoptosis', 'Carcinoma, Non-Small-Cell Lung', 'Cell Line, Tumor', 'Cell Movement', 'Cell Nucleus', 'Cell Proliferation', 'Cell Shape', 'Cell Surface Extensions', 'Cytoskeleton', 'Epithelial Cells', 'GTPase-Activating Proteins', 'Humans', 'Lung Neoplasms', 'Molecular Sequence Data', 'Mutant Proteins', 'Nuclear Localization Signals', 'Protein Structure, Tertiary', 'Protein Transport', 'Serine', 'Structure-Activity Relationship', 'Tumor Suppressor Proteins']
| 17,888,903
|
[['D05.750.078.730.250', 'D12.776.210.500.100', 'D12.776.220.525.255'], ['G02.111.570.060', 'L01.453.245.667.060'], ['G04.146.954.035'], ['C04.588.894.797.520.109.220.249', 'C08.381.540.140.500', 'C08.785.520.100.220.500'], ['A11.251.210.190', 'A11.251.860.180'], ['G04.198', 'G07.568.500.180'], ['A11.284.430.106', 'A11.284.430.214.190.875.117'], ['G04.161.750', 'G07.345.249.410.750'], ['G04.320'], ['A11.284.180'], ['A11.284.430.214.190.750'], ['A11.436'], ['D12.644.360.325.150', 'D12.776.476.325.150'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.894.797.520', 'C08.381.540', 'C08.785.520'], ['L01.453.245.667'], ['D12.776.602'], ['D12.644.770.610', 'G02.111.570.060.670.610'], ['G02.111.570.820.709.610'], ['G03.143.700'], ['D12.125.154.800'], ['G02.111.830', 'G07.690.773.997'], ['D12.776.624.776']]
|
['Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Information Science [L]', 'Diseases [C]', 'Anatomy [A]', 'Organisms [B]']
| 1
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Drawing as Instrument, Drawings as Evidence: Capturing Mental Processes with Pencil and Paper.
|
Researchers in the mind sciences often look to the production and analysis of drawings to reveal the mental processes of their subjects. This essay presents three episodes that trace the emergence of drawing as an instrumental practice in the study of the mind. Between 1880 and 1930, drawings gained currency as a form of scientific evidence - as stable, reproducible signals from a hidden interior. I begin with the use of drawings as data in the child study movement, move to the telepathic transmission of drawings in psychical research and conclude with the development of drawing as an experimental and diagnostic tool for studying neurological impairment. Despite significant shifts in the theoretical and disciplinary organisation of the mind sciences in the early twentieth century, researchers attempted to stabilise the use of subject-generated drawings as evidence by controlling the contexts in which drawings were produced and reproduced, and crafting subjects whose interiority could be effectively circumscribed. While movements such as psychoanalysis and art therapy would embrace the narrative interpretation of patient art, neuropsychology continued to utilise drawings as material traces of cognitive functions.
|
['Art', 'Art Therapy', 'Child', 'Female', 'History, 19th Century', 'History, 20th Century', 'Humans', 'Male', 'Mental Processes', 'Parapsychology', 'Psychology, Developmental']
| 27,292,325
|
[['K01.093'], ['E02.190.888.124', 'E02.760.169.063.500.100', 'E02.831.100', 'F04.754.070'], ['M01.060.406'], ['K01.400.504.937'], ['K01.400.504.968'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F02.463'], ['F02.550', 'F04.096.462', 'H01.770.644.364'], ['F04.096.628.270']]
|
['Humanities [K]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]', 'Named Groups [M]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
|
Age-dependent bupivacaine-induced muscle toxicity during continuous peripheral nerve block in rats.
|
BACKGROUND: Regional blocks improve postoperative analgesia and postoperative rehabilitation in children and adult patients. Continuous peripheral nerve blocks have been proposed as safe and effective techniques for postoperative pain relief and chronic pain therapy, particularly in small children. Few clinical reports have described myotoxicity induced by bupivacaine in these young patients, in contrast with a larger number of observations in adults. Here, the authors addressed this issue by a comparative evaluation of bupivacaine-induced myotoxicity in young versus adult rats.METHODS: Femoral nerve block catheters were inserted in male Wistar rats. Young (3-week-old) and adult (12-week-old) rats were randomly assigned to received seven injections (1 ml/kg) of 0.25% bupivacaine (n = 6 per experiment) or isotonic saline (n = 6 per experiment) at 8-h intervals. Rats were killed 8 h after the last injection. Psoas muscle adjacent to the femoral nerve was quickly dissected. Oxygen consumption rates were measured in saponin-skinned fibers, mitochondrial adenosine triphosphate synthesis rates were determined by bioluminescence, and citrate synthase activity was determined by spectrophotometry. Muscle ultrastructural damage was also examined and scored as normal, focal disruption, moderate disruption, or extreme disruption of the sarcomeres.RESULTS: Bupivacaine caused a reduction of mitochondrial adenosine triphosphate synthesis rate, a decrease of citrate synthase activity, and muscle ultrastructural damages. Young rats treated with bupivacaine showed more severe alterations of mitochondrial bioenergetics and muscle ultrastructure.CONCLUSIONS: These findings demonstrate that bupivacaine-induced myotoxicity can be explained by mitochondrial bioenergetics alterations, which are more severe in young rats.
|
['Age Factors', 'Anesthetics, Local', 'Animals', 'Bupivacaine', 'Energy Metabolism', 'Male', 'Muscle Fibers, Skeletal', 'Muscle, Skeletal', 'Nerve Block', 'Rats', 'Rats, Wistar']
| 19,809,284
|
[['N05.715.350.075', 'N06.850.490.250'], ['D27.505.696.277.100.200', 'D27.505.696.663.850.025', 'D27.505.954.427.210.100.200'], ['B01.050'], ['D02.065.199.239', 'D02.092.146.113.239'], ['G03.295'], ['A10.690.552.500.500', 'A11.620.249'], ['A02.633.567', 'A10.690.552.500'], ['E03.155.086.711', 'E04.525.210.550'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.150.900.649.313.992.635.505.700.900']]
|
['Health Care [N]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
Effect of thiol compounds on human complement component C4.
|
Thiol compounds have been investigated as inhibitors of the covalent binding reaction of human complement protein C4 using Sepharose-C1s as a combined activating and binding surface. o- and p-substituted aminothiophenols are equally effective inhibitors, whereas the m-substituted compound is a less potent inhibitor. The anti-hypertensive drug captopril is also shown to inhibit the covalent binding reaction. A comparison of the effects of these compounds on the covalent binding reaction of isolated C4A and C4B has been made. Results suggest that a Pro-to-Leu substitution in C4B is likely to account for the differences in inhibitory potency of C4B compared with C4A observed with the aromatic inhibitors.
|
['Aminophenols', 'Binding, Competitive', 'Captopril', 'Complement C1s', 'Complement C4', 'Complement C4a', 'Complement C4b', 'Complement Pathway, Classical', 'Humans', 'Isomerism', 'Penicillamine', 'Sepharose', 'Structure-Activity Relationship', 'Sulfhydryl Compounds']
| 8,435,078
|
[['D02.092.146.100', 'D02.455.426.559.389.657.050'], ['E05.196.080', 'G02.111.084', 'G02.111.570.120.309'], ['D12.125.072.401.623.270'], ['D08.811.277.300.290', 'D12.776.124.486.274.045.290', 'D12.776.124.486.274.050.290'], ['D12.776.124.486.274.350'], ['D12.776.124.486.274.024.260', 'D12.776.124.486.274.350.250'], ['D12.776.124.486.274.350.260'], ['G12.274.698'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G02.111.570.685', 'G02.607.445'], ['D02.886.030.786', 'D12.125.166.786'], ['D09.698.813'], ['G02.111.830', 'G07.690.773.997'], ['D02.886.489']]
|
['Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Regulation of bronchomotor tone in conscious calves.
|
The purpose of this study was to investigate the effects of some alpha and beta sympathomimetic and sympatholytic drugs on respiratory impedance in healthy conscious calves. Ten Friesian calves were investigated in this study. The forced oscillation technique was used to measure the resistance (Rrs) and the reactance (Xrs) of the respiratory system at frequencies ranging from 4 to 26 Hz. Isoprenaline (1 microgram/kg i.v.), propranolol (3 micrograms/kg i.v.), noradrenaline (2 micrograms/kg i.v.), xylazine (20 micrograms/kg i.v.) and yohimbine (0.25 mg/kg i.v.) were were administered. Isoprenaline induced a significant decrease of Rrs. An increase of Rrs after administration of propranolol was observed but without any change of the frequency dependence of Rrs. A small increase in the resonant frequency was also recorded. A decrease of Rrs was recorded after yohimbine injection. Noradrenaline and xylazine administration increased the resistances and the resonant frequency and induced a negative frequency dependence of Rrs. These results suggest that (1) the major effects of beta adrenergic drugs are on the central airways, (2) the alpha adrenergic system may play a role on the regulation of bronchomotor tone in calves, (3) the effects of alpha adrenergic drugs are on both central and peripheral airways and (4) the forced oscillation technique allows the differentiation of calibre changes occurring in small and large airways.
|
['Airway Resistance', 'Animals', 'Cattle', 'Isoproterenol', 'Norepinephrine', 'Propranolol', 'Respiratory Physiological Phenomena', 'Respiratory System', 'Sympatholytics', 'Sympathomimetics', 'Xylazine', 'Yohimbine']
| 2,704,063
|
[['E01.370.386.700.050', 'G09.772.060'], ['B01.050'], ['B01.050.150.900.649.313.500.380.271'], ['D02.033.100.291.439', 'D02.092.063.291.439', 'D02.092.311.649', 'D02.455.426.559.389.657.166.175.649'], ['D02.033.100.291.502', 'D02.092.063.480', 'D02.092.211.215.746', 'D02.092.311.830', 'D02.455.426.559.389.657.166.175.830'], ['D02.033.100.624.698.711', 'D02.033.755.624.698.711', 'D02.092.063.624.698.711', 'D02.455.426.559.847.638.945', 'D04.615.638.945'], ['G09.772'], ['A04'], ['D27.505.696.663.050.850'], ['D27.505.696.663.050.870'], ['D02.886.665.985', 'D03.383.855.985'], ['D03.132.436.681.933', 'D03.633.100.473.402.681.933', 'D03.633.100.496.500.500.681.933']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Chemicals and Drugs [D]', 'Anatomy [A]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Basal Ganglia Calcification: A Case Report of Fahr Disease With Pure Psychiatric Symptoms.
|
Fahr disease, also known as familial idiopathic basal ganglia calcification, is a rare neurodegenerative disorder, the etiology of which remains unknown. Given its various presentations, Fahr disease is presumed to be underdiagnosed and its prevalence underestimated. We present a case of Fahr disease that presented mainly with pure psychiatric symptoms. Isolated psychiatric symptoms without neurological manifestations are rarely seen in patients diagnosed with Fahr disease. Psychiatrists should consider Fahr disease as a differential diagnosis in the evaluation of psychiatric illness.
|
['Basal Ganglia', 'Basal Ganglia Diseases', 'Calcinosis', 'Depression', 'Diagnosis, Differential', 'Female', 'Humans', 'Mental Disorders', 'Middle Aged', 'Neurodegenerative Diseases', 'Olanzapine', 'Paroxetine', 'Psychiatric Status Rating Scales', 'Psychotropic Drugs', 'Tomography, X-Ray Computed', 'Treatment Outcome']
| 31,505,526
|
[['A08.186.211.200.885.287.249'], ['C10.228.140.079'], ['C18.452.174.130'], ['F01.145.126.350'], ['E01.171'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F03'], ['M01.060.116.630'], ['C10.574'], ['D03.633.100.079.080.738'], ['D03.383.621.600'], ['F04.711.513.653'], ['D27.505.954.427.700'], ['E01.370.350.350.810', 'E01.370.350.600.350.700.810', 'E01.370.350.700.700.810', 'E01.370.350.700.810.810', 'E01.370.350.825.810.810'], ['E01.789.800', 'N04.761.559.590.800', 'N05.715.360.575.575.800']]
|
['Anatomy [A]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Organisms [B]', 'Named Groups [M]', 'Chemicals and Drugs [D]', 'Health Care [N]']
| 1
| 1
| 1
| 1
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Decreased activity and impaired hormonal control of protein phosphatases in rat livers with a deficiency of phosphorylase kinase.
|
1. Livers from gsd/gsd rats, which do not express phosphorylase kinase activity, also contain much less particulate type-1 protein phosphatases. In comparison with normal Wistar rats, the glycogen/microsomal fraction contained 75% less glycogen-synthase phosphatase and 60% less phosphorylase phosphatase activity. This was largely due to a lower amount of the type-1 catalytic subunit in the particulate fraction. In the cytosol, the synthase phosphatase activity was also 50% lower, but the phosphorylase phosphatase activity was equal. 2. Both Wistar rats and gsd/gsd rats responded to an intravenous injection of insulin plus glucose with an acute increase (by 30-40%) in the phosphorylase phosphatase activity in the liver cytosol. In contrast, administration of glucagon or vasopressin provoked a rapid fall (by about 25%) in the cytosolic phosphorylase phosphatase activity in Wistar rats, but no change occurred in gsd/gsd rats. 3. Phosphorylase kinase was partially purified from liver and subsequently activated. Addition of a physiological amount of the activated enzyme to a liver cytosol from Wistar rats decreased the V of the phosphorylase phosphatase reaction by half, whereas the non-activated kinase had no effect. The kinase preparations did not change the activity of glycogen-synthase phosphatase, which does not respond to glucagon or vasopressin. Furthermore, the phosphorylase phosphatase activity was not affected by addition of physiological concentrations of homogeneous phosphorylase kinase from skeletal muscle (activated or non-activated). 4. It appears therefore that phosphorylase kinase plays an essential role in the transduction of the effect of glucagon and vasopressin to phosphorylase phosphatase. However, this inhibitory effect either is specific for the hepatic phosphorylase kinase, or is mediated by an unidentified protein that is a specific substrate of phosphorylase kinase.
|
['Animals', 'Cytosol', 'Enzyme Activation', 'Glucagon', 'Glucose', 'Glycogen Synthase', 'Glycogen-Synthase-D Phosphatase', 'Insulin', 'Kinetics', 'Liver', 'Microsomes, Liver', 'Phosphoprotein Phosphatases', 'Phosphorylase Kinase', 'Rats', 'Rats, Inbred Strains', 'Rats, Mutant Strains', 'Tissue Extracts', 'Vasopressins']
| 2,557,839
|
[['B01.050'], ['A11.284.430.214.200', 'A11.284.430.429.200', 'A11.284.835.450.200'], ['G02.111.263', 'G03.328'], ['D06.472.699.587.730.500', 'D12.644.548.586.730.500'], ['D09.947.875.359.448'], ['D08.811.913.400.450.460.375'], ['D08.811.277.352.650.625.300'], ['D06.472.699.587.200.500.625', 'D12.644.548.586.200.500.625'], ['G01.374.661', 'G02.111.490'], ['A03.620'], ['A11.284.835.540.541'], ['D08.811.277.352.650.625'], ['D08.811.913.696.620.682.650'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400'], ['B01.050.150.900.649.313.992.635.505.700.550'], ['D20.777'], ['D06.472.699.631.692.781', 'D12.644.400.900', 'D12.644.456.925', 'D12.644.548.691.692.781', 'D12.776.631.650.937']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Relationship between C-reactive protein, systemic oxygen consumption, and delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage.
|
BACKGROUND AND PURPOSE: Subarachnoid hemorrhage (SAH) is known to result in elevated systemic oxygen consumption (Vo(2)) and increases in high-sensitivity C-reactive protein (hsCRP), although the relationship among hsCRP, Vo(2), and delayed cerebral ischemia (DCI) after SAH remains unknown. We hypothesized that hsCRP is directly associated with Vo(2) and that elevated Vo(2) is a predictor of DCI after SAH.METHODS: Prospective serial assessments of Vo(2) and hsCRP over 4 prespecified time periods during the first 14 days after bleed in consecutive SAH patients admitted to a single academic medical center for a 2-year period.RESULTS: One hundred ten SAH patients met study criteria (mean age, 55±16 years; 62% women), with a median admission Hunt Hess grade of 3 (interquartile range, 2-4). In multivariate generalized estimating equation model of the first 14 days after bleed, Vo(2) was associated with younger age (P=0.01), male gender (P=0.01), and hsCRP levels (P=0.03). Twenty-four (22%) patients had DCI develop, with a median onset on day 7 after bleed (interquartile range, 5-11). The mean Vo(2) (291±65 mL/min versus 226±55 mL/min; P=0.003) was higher in DCI patients. In a multivariable Cox proportional hazards model, younger age (hazard ratio, 1.2 per 5 years; 95% CI, 1.1-1.3), a higher modified Fisher scale score (hazard ratio, 3.4 per 1-point increase; 95% CI, 1.7-6.9), and higher Vo(2) (HR, 1.2 per 50-mL/min increase; 95% CI, 1.1-1.3) were predictive of DCI.CONCLUSIONS: Systemic oxygen consumption is associated with hsCRP levels in the first 14 days after SAH and is an independent predictor of DCI.
|
['Adult', 'Aged', 'Brain Ischemia', 'C-Reactive Protein', 'Female', 'Humans', 'Intracranial Aneurysm', 'Male', 'Middle Aged', 'Oxygen Consumption', 'Prospective Studies', 'Subarachnoid Hemorrhage']
| 21,757,662
|
[['M01.060.116'], ['M01.060.116.100'], ['C10.228.140.300.150', 'C14.907.253.092'], ['D12.776.034.145', 'D12.776.124.050.120', 'D12.776.124.486.157'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C10.228.140.300.510.600', 'C14.907.055.635', 'C14.907.253.560.300'], ['M01.060.116.630'], ['G03.680'], ['E05.318.372.500.750.625', 'N05.715.360.330.500.750.650', 'N06.850.520.450.500.750.650'], ['C10.228.140.300.535.800', 'C14.907.253.573.800', 'C23.550.414.913.850']]
|
['Named Groups [M]', 'Diseases [C]', 'Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Copper-catalyzed Hiyama coupling of (hetero)aryltriethoxysilanes with (hetero)aryl iodides.
|
A Cu(I)-catalyzed Hiyama coupling was achieved, which proceeds in the absence of an ancillary ligand for aryl-heteroaryl and heteroaryl-heteroaryl couplings. A P,N-ligand is required to obtain the best product yields for aryl-aryl couplings. In addition to facilitating transmetalation, CsF is also found to function as a stabilizer of the [CuAr] species, potentially generated as an intermediate after transmetalation of aryltriethoxysilanes with Cu(I)-catalysts in the absence of ancillary ligands.
|
['Catalysis', 'Copper', 'Hydrocarbons, Iodinated', 'Iodides', 'Molecular Structure', 'Silanes']
| 24,079,478
|
[['G02.130'], ['D01.268.556.195', 'D01.268.956.170', 'D01.552.544.195'], ['D02.455.526.581'], ['D01.248.497.158.490', 'D01.475.410'], ['G02.111.570', 'G02.466'], ['D01.837.700']]
|
['Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 0
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Glial glucocorticoid receptors in aged Fisher 344 (F344) and F344/Brown Norway rats.
|
Glucocorticoid receptors (GR) regulate glial function, and changes in astrocyte gene expression are implicated in age-related pathology. We evaluated changes in astroglial GR expression in two strains of rats--Fisher 344 (F344; 4, 12 and 24 months) and F344/Brown Norway strain (F344/BN; 4, 12 and 30 months). In both strains basal levels of corticosterone were higher in the oldest groups of rats. Age-related increases in GR (+) astrocytes but not the percent of astrocytes expressing GR were observed in the hippocampus CA1 region in F344 rats. Age-related decreases in CA1 GR (+) astrocytes and the percentage of GR (+) astrocytes were observed in the F344/BN strain only. Similar strain-specific changes were observed in the dentate gyrus. In the hypothalamic paraventricular nucleus: (1) F344 rats exhibited significant decreases in the overall number of glial profiles with age, (2) F344/BN rats exhibited decreases in the numbers of GR (+) astrocytes with aging and (3) the proportion of GR (+) astrocytes decreased in older F344/BN, but not F344 rats. Overall, the data demonstrate age- and strain-related alterations in GR astrocytic expression that may explain unique phenotypic differences in brain function observed in both strains.
|
['Aging', 'Animals', 'Gene Expression Regulation, Enzymologic', 'Hippocampus', 'Hydrocortisone', 'Hypothalamus', 'Immunohistochemistry', 'Male', 'RNA, Messenger', 'Rats', 'Rats, Inbred F344', 'Receptors, Glucocorticoid']
| 19,249,343
|
[['G07.345.124'], ['B01.050'], ['G05.308.320'], ['A08.186.211.180.405', 'A08.186.211.200.885.287.500.345'], ['D04.210.500.745.745.654.600', 'D06.472.040.585.353.476', 'D06.472.040.585.478.392'], ['A08.186.211.180.497', 'A08.186.211.200.317.357'], ['E01.370.225.500.607.512', 'E01.370.225.750.551.512', 'E05.200.500.607.512', 'E05.200.750.551.512', 'E05.478.583', 'H01.158.100.656.234.512', 'H01.158.201.344.512', 'H01.158.201.486.512', 'H01.181.122.573.512', 'H01.181.122.605.512'], ['D13.444.735.544'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760.200', 'B01.050.150.900.649.313.992.635.505.700.400.200'], ['D12.776.826.750.430']]
|
['Phenomena and Processes [G]', 'Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]']
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Does postural asymmetry indicate directionally of rotation in rats: role of sex and handling.
|
Postural asymmetry of male and female Wistar rats was assessed during 4 tests: (a) tail suspension, (b) tail holding, (c) tail pinch and (d) walking on a narrow path. The tests were conducted prior to and after (+)-amphetamine (1 mg/kg, i.p.) administration. Rotational directionality was assessed in a rotometer. Only in female rats did postural adjustment during the tail-pinch predict with borderline significance the direction of circling prior to and after amphetamine. In both surgically castrated and conjugated estrogen-treated male rats, in sham-castrated and handled males, the tail-pinch test caused the same dominant directionality as rotation under amphetamine. The duplicity of postural tests suggests that there is a multitude of systems controlling and modulating postural response.
|
['Animals', 'Corpus Striatum', 'Dextroamphetamine', 'Dominance, Cerebral', 'Female', 'Handling, Psychological', 'Humans', 'Male', 'Motor Activity', 'Posture', 'Rats', 'Receptors, Dopamine', 'Sex Factors', 'Stereotyped Behavior', 'Substantia Nigra']
| 7,194,682
|
[['B01.050'], ['A08.186.211.200.885.287.249.487'], ['D02.092.471.683.152.110.200'], ['F02.830.297', 'G11.561.225'], ['F01.658.556'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['F01.145.632', 'G11.427.410.698'], ['G11.427.695'], ['B01.050.150.900.649.313.992.635.505.700'], ['D12.776.543.750.670.300.400', 'D12.776.543.750.695.150.400', 'D12.776.543.750.720.330.400'], ['N05.715.350.675', 'N06.850.490.875'], ['F01.145.896'], ['A08.186.211.132.659.413.656']]
|
['Organisms [B]', 'Anatomy [A]', 'Chemicals and Drugs [D]', 'Psychiatry and Psychology [F]', 'Phenomena and Processes [G]', 'Health Care [N]']
| 1
| 1
| 0
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
|
RhoScope: a highly portable computer program for visualization of the zero-flux atomic surfaces.
|
Atomic boundaries are defined within the topological theory of atoms in molecules as zero-flux surfaces in the gradient of electron density. The so-defined atomic surfaces often have quite complicated shapes that reflect the local characteristics of the electron distribution. A highly portable computer visualization program, called RhoScope, that displays the zero-flux atomic surfaces is described in this article. Examples of atomic surfaces in the C60 cluster and the C2H2LiCl carbenoid, rendered with the help of RhoScope, are presented.
|
['Carbon', 'Computer Graphics', 'Electrons', 'Models, Molecular', 'Software', 'Surface Properties']
| 8,011,600
|
[['D01.268.150'], ['L01.224.108', 'L01.296.110'], ['G01.249.335', 'G01.358.500.750'], ['E05.599.595'], ['L01.224.900'], ['G02.860']]
|
['Chemicals and Drugs [D]', 'Information Science [L]', 'Phenomena and Processes [G]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 0
| 0
| 0
|
Can standard rheumatology clinical practice be patient-based?
|
Patient-reported outcome measures (PROMs) are an attractive option in standard clinical practice. However, they do need to be validated on a broader spectrum of disease activity. Another factor to consider is patient adherence to treatment programmes which is directly linked to their satisfaction with clinical outcome. A recently developed multidimensional health assessment questionnaire has proved to be valid, save time and provide permanent medical and medico-legal documentation of the patient status at a given time. The questionnaire gives patients the opportunity to record their joint pain from their own perspective. The aim of this work was to examine whether the patient's self-assessment of tender joint counts agrees with the physician's evaluation, and whether it correlates with other disease activity parameters.
|
['Activities of Daily Living', 'Adult', 'Aged', 'Arthritis, Rheumatoid', 'Attitude of Health Personnel', 'Female', 'Health Status', 'Humans', 'Male', 'Middle Aged', 'Nursing Evaluation Research', 'Outcome Assessment, Health Care', 'Pain Measurement', 'Patient Participation', 'Patient-Centered Care', 'Physicians', 'Rheumatology', 'Severity of Illness Index', 'Surveys and Questionnaires']
| 18,563,011
|
[['E02.760.169.063.500.067', 'E02.831.067', 'I03.050', 'N02.421.784.110'], ['M01.060.116'], ['M01.060.116.100'], ['C05.550.114.154', 'C05.799.114', 'C17.300.775.099', 'C20.111.199'], ['F01.100.050', 'N05.300.100'], ['I01.240.425', 'N01.224.425', 'N06.850.505.400.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['H01.770.644.145.390.432', 'H02.478.395.432', 'N04.590.233.508.613.432'], ['H01.770.644.145.431', 'N04.761.559.590', 'N05.715.360.575.575'], ['E01.370.600.550.324'], ['F01.100.150.750.500.620', 'F01.145.488.887.500.620', 'N02.421.143.212.300', 'N03.540.245.360.300', 'N05.300.150.800.500.620'], ['N04.590.233.727.407'], ['M01.526.485.810', 'N02.360.810'], ['H02.403.429.730'], ['E05.318.308.980.438.475.456.500', 'N05.715.360.300.800.438.375.364.500', 'N06.850.520.308.980.438.475.364.500'], ['E05.318.308.980', 'N05.715.360.300.800', 'N06.850.520.308.980']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anthropology, Education, Sociology, and Social Phenomena [I]', 'Health Care [N]', 'Named Groups [M]', 'Diseases [C]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Disciplines and Occupations [H]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 1
| 0
|
["Pathomorphosis" of skin melanomas in the comprehensive treatment using hyperthermia].
|
The writers examined histologically and histochemically the melanomas removed in 40 patients, subjected to general and local hyperthermia associated with radiation and chemotherapy. In such cases it was found that melanoma tissues exhibit grave destructive changes characterized by the morphological features, which are manifested by microcirculatory disorders in the tumor and metastases and are associated with extensive injuries of the neoplastic tissue.
|
['Drug Therapy, Combination', 'Humans', 'Hyperthermia, Induced', 'Melanoma', 'Necrosis', 'Neoplasm Metastasis', 'Radiotherapy', 'Skin Neoplasms', 'Time Factors']
| 425,393
|
[]
|
[]
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
The role of nitric oxide formation in organic nitrate-induced vasodilation and organic nitrate tolerance.
|
Isolated rabbit aortic strips (RAS) were contracted submaximally with phenylephrine (PE) and then were incubated with 6.2 x 10(-7) M [3H] glyceryl trinitrate (GTN) (9.98 Ci/mmol) in a 30-s time-course study. GTN-induced relaxation of RAS was monitored and tissue GTN and GDN concentrations were determined by thin-layer chromatography and liquid scintillation spectrometry. There was time-dependent biotransformation of GTN to glyceryl dinitrate (GDN) by the RAS and a time-dependent increase in cyclic GMP content in the RAS. Statistically significant (P less than 0.05) biotransformation of GTN and elevation of cyclic GMP content in the tissue were found at 10 s in RAS, whereas the onset of relaxation occurred at 12 s. During the tissue biotransformation of GTN, there was preferential formation of 1,2-GDN compared with 1,3-GDN, suggesting that it is during the process of conversion of GTN to 1,2-GDN that elevation of cyclic GMP content occurs. The results of this study are consistent with the hypothesis that GTN is a prodrug, such that biotransformation to the active metabolite, nitric oxide (NO), is involved in GTN-induced relaxation of vascular smooth muscle. The data also indicate that the mechanism of GTN biotransformation and GTN-induced activation of guanylate cyclase may be related intimately. Isolated RAS were made tolerant to organic nitrates in vitro by incubation with 5 x 10(-4) M GTN for 1 h. After washout and submaximal contraction with PE, the tissues were incubated with 5 x 10(-7) M [14C]GTN for 2 min.(ABSTRACT TRUNCATED AT 250 WORDS)
|
['Animals', 'Aorta', 'Biotransformation', 'Cyclic GMP', 'Drug Tolerance', 'Muscle, Smooth, Vascular', 'Nitric Oxide', 'Nitroglycerin', 'Prodrugs', 'Rabbits', 'Vasodilation']
| 2,555,978
|
[['B01.050'], ['A07.015.114.056'], ['G03.171', 'G03.787.225', 'G07.690.725.225'], ['D03.633.100.759.646.454.160', 'D13.695.462.275', 'D13.695.667.454.160', 'D13.695.827.426.160'], ['G07.690.773.992'], ['A02.633.570.491', 'A07.015.733.500', 'A10.690.467.491'], ['D01.339.387', 'D01.625.550.500', 'D01.625.700.500', 'D01.650.550.587.600'], ['D02.640.636'], ['D26.675'], ['B01.050.150.900.649.313.968.700'], ['G09.330.380.928']]
|
['Organisms [B]', 'Anatomy [A]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Screening of BRCA1/2
|
BACKGROUND: Genetic analysis of BRCA1 and BRCA2 for the diagnosis of hereditary breast and ovarian cancer (HBOC) is commonly restricted to coding regions and exon-intron boundaries. Although germline pathogenic variants in these regions explain about ~20% of HBOC cases, there is still an important fraction that remains undiagnosed. We have screened BRCA1/2 deep intronic regions to identify potential spliceogenic variants that could explain part of the missing HBOC susceptibility.METHODS: We analysed BRCA1/2 deep intronic regions by targeted gene sequencing in 192 high-risk HBOC families testing negative for BRCA1/2 during conventional analysis. Rare variants (MAF <0.005) predicted to create/activate splice sites were selected for further characterisation in patient RNA. The splicing outcome was analysed by RT-PCR and Sanger sequencing, and allelic imbalance was also determined when heterozygous exonic loci were present.RESULTS: A novel transcript was detected in BRCA1 c.4185+4105C>T variant carrier. This variant promotes the inclusion of a pseudoexon in mature mRNA, generating an aberrant transcript predicted to encode for a non-functional protein. Quantitative and allele-specific assays determined haploinsufficiency in the variant carrier, supporting a pathogenic effect for this variant. Genotyping of 1030 HBOC cases and 327 controls did not identify additional carriers in Spanish population.CONCLUSION: Screening of BRCA1/2 intronic regions has identified the first BRCA1 deep intronic variant associated with HBOC by pseudoexon activation. Although the frequency of deleterious variants in these regions appears to be low, our study highlights the importance of studying non-coding regions and performing comprehensive RNA assays to complement genetic diagnosis.
|
['Adult', 'BRCA1 Protein', 'BRCA2 Protein', 'Breast Neoplasms, Male', 'Case-Control Studies', 'Computer Simulation', 'Exons', 'Female', 'Gene Expression Regulation', 'Gene Frequency', 'Genetic Testing', 'Germ-Line Mutation', 'Hereditary Breast and Ovarian Cancer Syndrome', 'Humans', 'Introns', 'Male', 'RNA Splicing', 'RNA, Messenger']
| 30,472,649
|
[['M01.060.116'], ['D12.776.313.125', 'D12.776.624.776.100', 'D12.776.660.100', 'D12.776.744.100', 'D12.776.930.137'], ['D12.776.313.249', 'D12.776.624.776.101', 'D12.776.660.105'], ['C04.588.180.260', 'C17.800.090.500.260'], ['E05.318.372.500.500', 'N05.715.360.330.500.500', 'N06.850.520.450.500.500'], ['L01.224.160'], ['G05.360.340.024.340.137.232'], ['G05.308'], ['G05.330'], ['E01.370.225.562', 'E05.200.562', 'E05.393.435', 'N02.421.308.430', 'N02.421.726.233.221'], ['G05.365.590.350'], ['C04.588.180.483', 'C04.588.322.455.431', 'C04.700.517', 'C13.351.500.056.630.705.431', 'C13.351.937.418.685.431', 'C16.320.700.517', 'C17.800.090.500.483', 'C19.344.410.431', 'C19.391.630.705.431'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['G05.360.340.024.220.400', 'G05.360.340.024.340.137.515'], ['G02.111.760.700', 'G03.839.700', 'G05.308.700.700'], ['D13.444.735.544']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Health Care [N]', 'Information Science [L]', 'Phenomena and Processes [G]', 'Organisms [B]']
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 1
| 1
| 1
| 0
|
Royal Jelly Abrogates Cadmium-Induced Oxidative Challenge in Mouse Testes: Involvement of the Nrf2 Pathway.
|
The current study examined the efficacy of royal jelly (RJ) against cadmium chloride (CdCl₂)-induced testicular dysfunction. A total of 28 Swiss male mice were allocated into four groups (n = 7), and are listed as follows: (1) the control group, who was intraperitoneally injected with physiological saline (0.9% NaCl) for 7 days; (2) the RJ group, who was orally supplemented with RJ (85 mg/kg daily equivalent to 250 mg crude RJ) for 7 days; (3) the CdCl₂ group, who was intraperitoneally injected with 6.5 mg/kg for 7 days; and (4) the fourth group, who was supplemented with RJ 1 h before CdCl₂ injection for 7 days. Cd-intoxicated mice exhibited a decrease in serum testosterone, luteinizing hormone (LH), and follicle stimulating hormone (FSH) levels. A disturbance in the redox status in the testicular tissue was recorded, as presented by the increase in lipid peroxidation and nitrate/nitrite levels and glutathione (GSH) depletion. Moreover, the activities of glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), and nuclear factor (erythroid-derived 2)-like-2 factor (Nrf2) and their gene expression were inhibited. In addition, interleukin-1? (IL-1â) and tumor necrosis factor-á (TNF-á) levels were elevated. Furthermore, Cd triggered an apoptotic cascade via upregulation of caspase-3 and Bax and downregulation of Bcl-2. Histopathological examination showed degenerative changes in spermatogenic cells, detachment of the spermatogenic epithelium from the basement membrane, and vacuolated seminiferous tubules. Decreased cell proliferation was reflected by a decrease in proliferating cell nuclear antigen (PCNA) expression. Interestingly, RJ supplementation markedly minimized the biochemical and molecular histopathological changes in testes tissue in response to Cd exposure. The beneficial effects of RJ could be attributed to its antioxidative properties.
|
['Animals', 'Apoptosis', 'Cadmium', 'Fatty Acids', 'Follicle Stimulating Hormone', 'Glutathione', 'Inflammation', 'Lipid Peroxidation', 'Luteinizing Hormone', 'Male', 'Mice', 'NF-E2-Related Factor 2', 'Nitrates', 'Nitrites', 'Organ Size', 'Oxidative Stress', 'RNA, Messenger', 'Signal Transduction', 'Testis', 'Testosterone']
| 30,544,760
|
[['B01.050'], ['G04.146.954.035'], ['D01.268.556.137', 'D01.268.956.061', 'D01.552.544.137'], ['D10.251'], ['D06.472.699.322.576.288', 'D06.472.699.631.525.343.288', 'D12.644.548.691.525.343.288'], ['D12.644.456.448'], ['C23.550.470'], ['G02.111.515', 'G03.295.531.587'], ['D06.472.699.322.576.463', 'D06.472.699.631.525.343.463', 'D12.644.548.691.525.343.463'], ['B01.050.150.900.649.313.992.635.505.500'], ['D12.776.260.108.737', 'D12.776.930.127.737'], ['D01.248.497.158.606', 'D01.625.525.550', 'D02.583'], ['D01.248.497.158.635', 'D01.625.600.600', 'D02.633'], ['E01.370.600.115.100.660', 'E05.041.124.715', 'G07.100.100.660', 'G07.345.249.690'], ['G03.673', 'G07.775.750'], ['D13.444.735.544'], ['G02.111.820', 'G04.835'], ['A05.360.444.849', 'A05.360.576.782', 'A06.300.312.782'], ['D04.210.500.054.079.429.824', 'D06.472.334.851.968.984']]
|
['Organisms [B]', 'Phenomena and Processes [G]', 'Chemicals and Drugs [D]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
A rare native mitral valve endocarditis successfully treated after surgical correction.
|
Mycobacterium abscessus and Kocuria species are rare causes of infections in humans. Endocarditis by these agents has been reported in only 11 cases. M. abscessus is a particularly resistant organism and treatment requires the association of antibiotics for a prolonged period of time. We report a case of native mitral valve bacterial endocarditis due to M. abscessus and Kocuria species in a 48-year-old man with a history of intravenous drug use. The case was complicated by a perforation of the posterior mitral valve leaflet, leading to surgical mitral valve replacement. Cultures from the blood and mitral valve disclosed M. abscessus and Kocuria species. The patient was treated for 6 months with clarithromycin, imipenem and amikacin, with resolution of symptoms. Repeated blood cultures were negative. Acid-fast staining should be done in subacute endocarditis in order to identify rapidly growing mycobacteria.
|
['Actinomycetales Infections', 'Endocarditis, Bacterial', 'Humans', 'Male', 'Micrococcaceae', 'Middle Aged', 'Mitral Valve Annuloplasty', 'Mitral Valve Insufficiency', 'Mycobacterium Infections, Nontuberculous', 'Rare Diseases', 'Substance Abuse, Intravenous']
| 25,270,154
|
[['C01.150.252.410.040'], ['C01.150.252.245', 'C01.190.249', 'C14.260.249', 'C14.280.282.407'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['B03.510.024.850', 'B03.510.400.500'], ['M01.060.116.630'], ['E04.100.376.062.500', 'E04.928.220.109.500'], ['C14.280.484.461'], ['C01.150.252.410.040.552.475'], ['C23.550.291.906'], ['C25.775.793', 'F03.900.793']]
|
['Diseases [C]', 'Organisms [B]', 'Named Groups [M]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Psychiatry and Psychology [F]']
| 0
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Suppression behavior analyzed as a function of monovision addition power.
|
Monovision, the use of a monocular addition for near viewing, is a clinical technique sometimes used to correct presbyopia. This technique attempts to maintain binocular function while requiring a degree of central suppression. We examined suppression behavior in spectacle monovision as a function of addition power. Suppression behavior was determined by measuring the length and frequency of periods of suppression for a range of monocular addition powers. Testing was conducted at viewing distances of 6 m and 40 cm. The most striking result is the existence of a near/distance differential: the monocular addition power required to stabilize suppression at near is greater than at distance. It was also found that suppression behavior may be stable even in the presence of a relatively high level of stereoscopic acuity. The relation between the contradictory needs for suppression and binocular functioning under monovision conditions is discussed.
|
['Adult', 'Distance Perception', 'Eyeglasses', 'Functional Laterality', 'Humans', 'Optics and Photonics', 'Presbyopia', 'Visual Acuity']
| 3,963,142
|
[['M01.060.116'], ['F02.463.593.200.390', 'F02.463.593.778.255.390'], ['E07.632.500.300'], ['F02.830.297.425', 'G11.561.225.425'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['H01.671.617', 'J01.293.688'], ['C11.744.786'], ['E01.370.380.850.950', 'F02.463.593.932.901', 'G14.940']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Disciplines and Occupations [H]', 'Technology, Industry, and Agriculture [J]', 'Diseases [C]']
| 0
| 1
| 1
| 0
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 1
| 0
| 0
|
Debaryomyces hansenii
|
The ability of Debaryomyces hansenii to produce volatile sulfur compounds from sulfur amino acids and the metabolic pathway involved have been studied in seven strains from different food origins. Our results proved that l-methionine is the main precursor for sulfur compound generation. Crucial differences in the sulfur compound profile and amino acid consumption among D. hansenii strains isolated from different food sources were observed. Strains isolated from dry pork sausages displayed the most complex sulfur compound profiles. Sulfur compound production, such as that of methional, could result from chemical reactions or yeast metabolism, while according to this study, thioester methyl thioacetate appeared to be generated by yeast metabolism. No relationship between sulfur compounds production by D. hansenii strains and the expression of genes involved in sulfur amino acid metabolism was found, except for the ATF2 gene in the L1 strain for production of methyl thioacetate. Our results suggest a complex scenario during sulfur compound production by D. hansenii.
|
['Amino Acids, Sulfur', 'Animals', 'Debaryomyces', 'Fermented Foods and Beverages', 'Fungal Proteins', 'Meat Products', 'Sulfur Compounds', 'Swine', 'Volatilization']
| 31,343,169
|
[['D02.886.030', 'D12.125.166'], ['B01.050'], ['B01.300.107.795.190', 'B01.300.930.323'], ['G07.203.200', 'J02.350'], ['D12.776.354'], ['G07.203.300.600.500', 'J02.500.600.500'], ['D01.875', 'D02.886'], ['B01.050.150.900.649.313.500.880'], ['G01.645.750', 'G02.734.933']]
|
['Chemicals and Drugs [D]', 'Organisms [B]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 0
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
|
Bold or reckless? The impact of workplace risk-taking on attributions and expected outcomes.
|
Risk-takers are rhetorically extolled in America, but does this veneration ignore the downsides of failure? We test competing perspectives on how workplace risk-takers are perceived by examining cultural attitudes about individuals who successfully take, unsuccessful take, and avoid risks at work. The results of two experiments show that, in comparison to risk-avoidance, expected workplace outcomes are enhanced by successful risk-taking and that failure does not appear to significantly harm expected workplace outcomes for risk-takers. While one experiment finds that failed risk-takers are seen as more likely to be downsized (because they are viewed as more foolish), we also find failed risk-takers are perceived as more likely to be hired and promoted. Mediation analyses reveal this is primarily because risk-taking-regardless of outcome-considerably increases perceptions of agency and decreases perceptions of indecisiveness, and these attributions predict positive workplace outcomes. We also find the results to be remarkably similar across varying participant characteristics (namely, gender, race, education level, work experience, income, and age), which suggests that there is a broad cultural consensus in the U.S. about the value of risk-taking. In sum, we find evidence that observers generally make more positive attributions about risk-takers than about risk-avoiders, even when risk-takers fail.
|
['Adolescent', 'Adult', 'Aged', 'Attitude', 'Female', 'Humans', 'Male', 'Middle Aged', 'Personality', 'Personnel Selection', 'Psychological Tests', 'Random Allocation', 'Risk-Taking', 'Social Perception', 'Workplace', 'Young Adult']
| 32,130,225
|
[['M01.060.057'], ['M01.060.116'], ['M01.060.116.100'], ['F01.100'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['M01.060.116.630'], ['F01.752'], ['N04.452.677.500'], ['F04.711'], ['E05.318.370.700', 'E05.581.500.805', 'N05.715.360.325.675', 'N06.850.520.445.700'], ['F01.145.722'], ['F02.463.593.752'], ['N01.824.245.925', 'N04.452.677.975'], ['M01.060.116.815']]
|
['Named Groups [M]', 'Psychiatry and Psychology [F]', 'Organisms [B]', 'Health Care [N]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]']
| 0
| 1
| 0
| 0
| 1
| 1
| 0
| 0
| 0
| 0
| 0
| 1
| 1
| 0
|
Histochemistry of proteases in ependyma, choroid plexus and leptomeninges.
|
Aminopeptidase M (APM), aminopeptidase A (APA), dipeptidyl peptidase IV (DPP IV) and gamma-glutamyl transferase (GGT) were demonstrated histochemically in cryostat sections of the rat brain to show the reaction pattern of ependyma, choroid plexus and leptomeninges. GGT was only demonstrable in the cell membranes of ependymal cells and in the leptomeninges; however, APA, APM and DAP IV showed a variable degree of activity in the capillary endothelium of the choroid plexus as well as in the leptomeninges. On the basis of these results, it is postulated that peptides in the cerebrospinal fluid can be cleaved extraventricularly by the enzymes demonstrated in the leptomeninges.
|
['Animals', 'Brain', 'Choroid Plexus', 'Ependyma', 'Female', 'Histocytochemistry', 'Male', 'Meninges', 'Neuropeptides', 'Peptide Hydrolases', 'Rats', 'Rats, Inbred Strains']
| 3,284,853
|
[['B01.050'], ['A08.186.211'], ['A08.186.211.140.298'], ['A08.186.211.140.460', 'A10.755.260'], ['E01.370.225.500.607', 'E01.370.225.750.551', 'E05.200.500.607', 'E05.200.750.551', 'H01.158.100.656.234', 'H01.158.201.344', 'H01.181.122.573'], ['A08.186.566'], ['D12.644.400', 'D12.776.631.650'], ['D08.811.277.656'], ['B01.050.150.900.649.313.992.635.505.700'], ['B01.050.050.199.520.760', 'B01.050.150.900.649.313.992.635.505.700.400']]
|
['Organisms [B]', 'Anatomy [A]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Disciplines and Occupations [H]', 'Chemicals and Drugs [D]']
| 1
| 1
| 0
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
|
Clear cell sarcomas of the kidney are characterised by BCOR gene abnormalities, including exon 15 internal tandem duplications and BCOR-CCNB3 gene fusion.
|
AIMS: Clear cell sarcoma of the kidney (CCSK) is a rare paediatric renal malignant tumour. The majority of CCSKs have internal tandem duplications (ITDs) of the BCOR gene, whereas a minority have the YWHAE-NUTM2 gene fusion. A third 'double-negative' (DN) category comprises CCSKs with neither BCOR ITDs nor YWHAE-NUTM2 fusion. The aim of this study was to characterise 11 histologically diagnosed CCSKs immunohistochemically (with CCND1, BCOR and CCNB3 stains) and genetically.METHODS AND RESULTS: By next-generation sequencing, 10 cases (90.9%) had BCOR exon 15 ITDs, with positive BCOR immunoreactivity being found in four (36%) or eight (72%) cases, depending on the antibody clone. By reverse transcription polymerase chain reaction, none had the YWHAE-NUTM2 fusion. The DN case had a BCOR-CCNB3 fusion and strong nuclear CCNB3 and BCOR immunoreactivity. Quantitative polymerase chain reaction showed markedly elevated BCOR expression in this case, whereas BCOR ITD cases had lower levels of elevated BCOR expression.CONCLUSIONS: The majority of the CCSKs in our cohort had BCOR ITDs, and none had the YWHAE-NUTM2 fusion. We verified the strong, diffuse cyclin D1 (CCND1) immunoreactivity in CCSKs described in recent reports. BCOR immunoreactivity was not consistently positive in all CCSKs with BCOR ITDs, and therefore cannot be used as a diagnostic immunohistochemical stain to identify BCOR ITD cases. The DN case was a BCOR-CCNB3 fusion sarcoma. BCOR-CCNB3 sarcoma is typically a primary bone sarcoma affecting male adolescents, and this is the first report of it presenting in a kidney of a young child as a CCSK. The full spectrum of DN CCSKs awaits more comprehensive characterisation.
|
['Child', 'Child, Preschool', 'Cyclin B', 'Exons', 'Female', 'Humans', 'Kidney Neoplasms', 'Male', 'Oncogene Proteins, Fusion', 'Proto-Oncogene Proteins', 'Repressor Proteins', 'Sarcoma, Clear Cell']
| 28,833,375
|
[['M01.060.406'], ['M01.060.406.448'], ['D12.644.360.262.120', 'D12.776.167.218.120', 'D12.776.476.262.120'], ['G05.360.340.024.340.137.232'], ['B01.050.150.900.649.313.988.400.112.400.400'], ['C04.588.945.947.535', 'C12.758.820.750', 'C12.777.419.473', 'C13.351.937.820.535', 'C13.351.968.419.473'], ['D12.776.602.500.500', 'D12.776.624.664.500'], ['D12.776.624.664.700'], ['D12.776.260.703', 'D12.776.930.780'], ['C04.557.450.565.800', 'C04.557.450.795.800']]
|
['Named Groups [M]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Organisms [B]', 'Diseases [C]']
| 0
| 1
| 1
| 1
| 0
| 0
| 1
| 0
| 0
| 0
| 0
| 1
| 0
| 0
|
Effect of weaning on antibody responses and nematode parasitism in Merino lambs.
|
Lambs weaned at eight weeks old were compared with control lambs which remained with their dams; both groups grazed the same pasture. Weaning significantly reduced the growth rate, control lambs being, on average, 6 kg heavier than weaned lambs at 15 weeks old. When contamination of pasture with larval parasites was light, both groups of lambs suffered only modest parasitic infections. When lambs were experimentally infected with 5000 Haemonchus contortus and 10,000 Trichostrongylus colubriformis larvae at eight weeks old, the mean faecal egg count for weaned lambs was twice that for controls at 12 weeks old (P less than 0.001) and weaned lambs suffered a significantly greater decline in packed cell volume than controls over the next four weeks. Antibody responses following immunisation with either ovalbumin or Brucella abortus at four and at eight weeks old, did not differ significantly between control and weaned lambs. In contrast serum antibody responses to H contortus and T colubriformis differed significantly between the two groups, with controls responding earlier and more strongly than weaned lambs. The practical significance of these findings is that up to three months old, suckled lambs, when faced with a substantial parasite challenge, have much better prospects than weaned lambs.
|
['Animals', 'Antibodies, Helminth', 'Antibody Formation', 'Body Weight', 'Brucella abortus', 'Feces', 'Haemonchiasis', 'Haemonchus', 'Intestinal Diseases, Parasitic', 'Ovalbumin', 'Parasite Egg Count', 'Sheep', 'Sheep Diseases', 'Trichostrongylosis', 'Trichostrongylus', 'Weaning']
| 1,788,476
|
[['B01.050'], ['D12.776.124.486.485.114.185', 'D12.776.124.790.651.114.185', 'D12.776.377.715.548.114.185'], ['G12.450.050.370.250'], ['C23.888.144', 'E01.370.600.115.100.160.120', 'E05.041.124.160.750', 'G07.100.100.160.120', 'G07.345.249.314.120'], ['B03.440.400.425.215.500.100', 'B03.660.050.070.100.100'], ['A12.459'], ['C01.610.335.508.700.775.825.400'], ['B01.050.500.500.294.400.968.746.410'], ['C01.610.432', 'C06.405.469.452'], ['D12.644.861.557', 'D12.776.034.614', 'D12.776.256.159.157.663', 'D12.776.290.663', 'D12.776.872.557'], ['E01.370.225.932.600', 'E05.200.932.600'], ['B01.050.150.900.649.313.500.380.791'], ['C22.836'], ['C01.610.335.508.700.775.825.842'], ['B01.050.500.500.294.400.968.746.610'], ['G07.203.650.220.500.750', 'G07.203.650.915']]
|
['Organisms [B]', 'Chemicals and Drugs [D]', 'Phenomena and Processes [G]', 'Diseases [C]', 'Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Anatomy [A]']
| 1
| 1
| 1
| 1
| 1
| 0
| 1
| 0
| 0
| 0
| 0
| 0
| 0
| 0
|
Gold nanostars as thermoplasmonic nanoparticles for optical heating.
|
Gold nanostars are theoretically studied as efficient thermal heaters at their corresponding localized surface-plasmon resonances (LSPRs). Numerical calculations are performed through the 3D Green's Theorem method to obtain the absorption and scattering cross sections for Au nanoparticles with star-like shape of varying symmetry and tip number. Their unique thermoplasmonic properties, with regard to their (red-shifted) LSPR wavelentgh, (? 30-fold increase) steady-state temperature, and scattering/absorption cross section ratios, make them specially suitable for optical heating and in turn for cancer thermal therapy.
|
['Equipment Design', 'Equipment Failure Analysis', 'Gold', 'Heating', 'Hot Temperature', 'Light', 'Nanoparticles', 'Surface Plasmon Resonance']
| 22,274,385
|
[['E05.320'], ['E05.325.192'], ['D01.268.556.322', 'D01.268.956.186', 'D01.552.544.322'], ['N06.230.150.300'], ['G01.906.595.543', 'G16.500.275.063.725.710.380', 'G16.500.750.775.710.380', 'N06.230.300.100.725.232', 'N06.230.300.100.725.710.380'], ['G01.358.500.505.650', 'G01.590.540', 'G01.750.250.650', 'G01.750.770.578'], ['J01.637.512.600'], ['E05.196.890', 'E05.601.043.700']]
|
['Analytical, Diagnostic and Therapeutic Techniques, and Equipment [E]', 'Chemicals and Drugs [D]', 'Health Care [N]', 'Phenomena and Processes [G]', 'Technology, Industry, and Agriculture [J]']
| 0
| 0
| 0
| 1
| 1
| 0
| 1
| 0
| 0
| 1
| 0
| 0
| 1
| 0
|
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