"""Native ASCENT and ZephIR table adapters with conservative annotation provenance.""" from __future__ import annotations import numpy as np import pandas as pd XYZ = ["x_voxel", "y_voxel", "z_voxel"] TRUSTED = {"human_corrected", "manually_verified", "verified_checkpoint"} def _physical(df, spacing_xyz): for axis, scale in zip("xyz", spacing_xyz or [None] * 3, strict=True): df[f"{axis}_um"] = df[f"{axis}_voxel"] * scale if scale is not None else np.nan df["coordinate_system"] = "source_microscope" df["annotation_confidence"] = np.nan df["validity"] = True return df def ascent_tracks(path, recording_id, *, predicted=False): """Read native ZYX CSV; physical spacing is from Zenodo 17561700 metadata.""" df = pd.read_csv(path).rename( columns={ "TrackID": "track_id", "ObjectID": "detection_id", "t": "frame_index", "x": "x_voxel", "y": "y_voxel", "z": "z_voxel", } ) required = {"track_id", "detection_id", "frame_index", *XYZ} if not required.issubset(df.columns): raise ValueError(f"Missing ASCENT fields: {sorted(required - set(df.columns))}") df["recording_id"] = recording_id df["annotation_type"] = "algorithm_prediction" if predicted else "human_corrected" df["source_provenance"] = "ASCENT-PRED" if predicted else "ASCENT-GT-proofread-ZephIR" df["label_set"] = "prediction" if predicted else "reference" df["track_id"] = df.track_id.astype(str) df["detection_id"] = df.detection_id.map(lambda i: f"{'pred' if predicted else 'gt'}:{i}") return _physical(df, [0.243, 0.243, 1.5]) def zephir_tracks(path, metadata, recording_id): """Retain scorer codes; only documented ZEIR results are classified as predictions. Other codes remain unknown until their human/algorithm provenance is verified. Normalized coordinates map to voxel index x*size-0.5 using the native viewer convention. No voxel spacing is inferred from the neuron arrangement. """ import h5py with h5py.File(path, "r") as h: df = pd.DataFrame({k: h[k][()] for k in h}) df = df.rename( columns={ "t_idx": "frame_index", "worldline_id": "track_id", "id": "detection_id", "provenance": "source_provenance", } ) df["source_provenance"] = df.source_provenance.map(lambda s: s.decode("utf-8")) for axis in "xyz": df[f"{axis}_normalized"] = df[axis] df[f"{axis}_voxel"] = df[axis] * metadata[f"shape_{axis}"] - 0.5 df = df.drop(columns=axis) df["annotation_type"] = np.where( df.source_provenance == "ZEIR", "algorithm_prediction", "unknown" ) df["recording_id"] = recording_id df["label_set"] = "source_mixed" df["track_id"] = df.track_id.astype(str) df["detection_id"] = df.detection_id.map(lambda i: f"source:{i}") df = _physical(df, None) normalized = df[[f"{axis}_normalized" for axis in "xyz"]].to_numpy(dtype=float) df["validity"] = ( np.isfinite(normalized).all(axis=1) & (normalized >= 0).all(axis=1) & (normalized <= 1).all(axis=1) ) return df def validate_tracks(df, *, shape_xyz=None): """Reject ambiguous assignments and nonfinite observed points; never clamp or interpolate.""" required = { "recording_id", "frame_index", "track_id", "detection_id", *XYZ, "annotation_type", "label_set", "validity", } if not required.issubset(df.columns): raise ValueError(f"Missing fields: {sorted(required - set(df.columns))}") if df.empty: raise ValueError("Empty tracking table") valid = df.loc[df.validity] scope = ["recording_id", "label_set", "frame_index"] if valid.duplicated(scope + ["track_id"]).any(): raise ValueError("Duplicate track assignments") if valid.duplicated(scope + ["detection_id"]).any(): raise ValueError("Duplicate detection assignments") if valid.track_id.isna().any() or valid.detection_id.isna().any(): raise ValueError("Missing identity on a valid observation") if not np.isfinite(valid[XYZ].to_numpy(dtype=float)).all(): raise ValueError("Nonfinite observed coordinates") if (valid.frame_index < 0).any() or (valid.frame_index % 1 != 0).any(): raise ValueError("Invalid frame index") # Centers use pixel boundaries [-0.5, size-0.5], including ZephIR normalized endpoints. if shape_xyz is not None: points = valid[XYZ].to_numpy(dtype=float) if ((points < -0.5) | (points > np.asarray(shape_xyz) - 0.5)).any(): raise ValueError("Spatial coordinates outside source bounds") return { "observations": len(valid), "frames": int(valid.frame_index.nunique()), "tracks": int(valid.track_id.nunique()), "annotation_types": { str(k): int(v) for k, v in valid.annotation_type.value_counts().items() }, }