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0003082 | Section title: Introduction Educational score: 4.3566813468933105 Domain: biomedical Document type: Study Language: en The functioning of the nervous system depends upon an immensely complex and precise network of neurons. Cell adhesion is thought to play a critical role in the formation and maintenance of synaptic con... | Study | biomedical | en | 0.999997 |
0003113 | Section title: Introduction Educational score: 4.189830303192139 Domain: biomedical Document type: Study Language: en Many pathologic stimuli induce the heart to undergo adaptive hypertrophic growth that temporarily augments cardiac function. Although the initial hypertrophic response may be beneficial, sustained hyper... | Study | biomedical | en | 0.999998 |
0003137 | Section title: Introduction Educational score: 4.197197914123535 Domain: biomedical Document type: Other Language: en A particular shape and a well-defined polarity are characteristic of many cell types, as illustrated by the extended morphology of differentiated nerve cells or the asymmetric organization of polarized ... | Other | biomedical | en | 0.999998 |
0004087 | Section title: Introduction Educational score: 4.173458576202393 Domain: biomedical Document type: Study Language: en Dynamic regulation of the actin cytoskeleton plays a central role in a variety of cellular events, including adhesion, division, spreading, and motility . Engagement or activation of cell surface growth... | Study | biomedical | en | 0.999996 |
0004096 | Section title: Introduction Educational score: 4.406662464141846 Domain: biomedical Document type: Study Language: en Messenger RNA degradation is a mechanism by which eukaryotic cells regulate gene expression and influence cell growth and differentiation . It is dependent upon both cis-elements in the RNA and trans-ac... | Study | biomedical | en | 0.999997 |
0004111 | Section title: Introduction Educational score: 4.75501823425293 Domain: biomedical Document type: Study Language: en Receptor-mediated endocytosis for recycling receptors is a process in which a ligand binds to its cell surface receptor and is internalized via clathrin-coated vesicle formation . After uncoating, this v... | Study | biomedical | en | 0.999998 |
0005018 | Section title: Introduction Educational score: 4.580744743347168 Domain: biomedical Document type: Study Language: en The ends of eukaryotic chromosomes (telomeres) consist of simple DNA repeats associated with proteins and play important roles in replication, malignant transformation, and cellular aging . Besides the ... | Study | biomedical | en | 0.999997 |
0005026 | Section title: Introduction Educational score: 4.591047286987305 Domain: biomedical Document type: Study Language: en The congression of chromosomes to the metaphase plate and subsequent poleward movement at anaphase are complex processes that occur with remarkable accuracy during cell division. An important organelle ... | Study | biomedical | en | 0.999998 |
0005034 | Section title: Introduction Educational score: 3.892241954803467 Domain: biomedical Document type: Study Language: en Differentiated oligodendrocytes synthesize large amounts of myelin, a multilamellar membrane that ensheathes and insulates the axons of the central nervous system . The specific molecular organization o... | Study | biomedical | en | 0.999997 |
0006005 | Section title: Introduction Educational score: 4.429399490356445 Domain: biomedical Document type: Study Language: en A significant component of eukaryotic cellular protein degradation occurs at the ER. ER-associated degradation (ERAD) is widely employed in cellular quality control, resulting in the destruction of nume... | Study | biomedical | en | 0.999997 |
0006072 | Section title: Introduction Educational score: 4.626532554626465 Domain: biomedical Document type: Study Language: en It is generally believed that proteins are transported through a protein-conducting channel of the ER membrane in an unfolded conformation. In cotranslational translocation, an unfolded state is maintai... | Study | biomedical | en | 0.999996 |
0006125 | Section title: Introduction Educational score: 4.311325550079346 Domain: biomedical Document type: Study Language: en Mammalian cells internalize a large quantity of plasma membrane-associated and soluble molecules, and sort these molecules into recycling or lysosomal pathways. A great deal of this sorting occurs in ea... | Study | biomedical | en | 0.999997 |
0006158 | Section title: Introduction Educational score: 4.778325080871582 Domain: biomedical Document type: Review Language: en Agrin is a heparan sulfate proteoglycan that was purified from basal lamina (BL) based upon its ability to induce clustering of acetylcholine receptors on cultured myotubes . It has since been shown to... | Review | biomedical | en | 0.999998 |
10036122 | Section title: INTRODUCTION Educational score: 4.254120826721191 Domain: biomedical Document type: Review Language: en Changes induced by the currently used raw gas during insufflation to create a pneumoperitoneum induces laparoscopic hypothermia, causes postoperative pain and results in prolonged recovery room stay. 1... | Review | biomedical | en | 0.999997 |
10036123 | Section title: INTRODUCTION Educational score: 4.09364128112793 Domain: biomedical Document type: Review Language: en The incidence of adenocarcinoma of the esophagus is rising at an alarming rate. 1 The prognosis is poor, with a five year survival of 5-10%. Randomized studies indicate that multimodality regimens have ... | Review | biomedical | en | 0.999997 |
10036124 | Section title: INTRODUCTION Educational score: 3.831684112548828 Domain: biomedical Document type: Clinical case Language: en Double gallbladder is a rare anomaly of the biliary tract, occurring in about 1 per 3800 cases at autopsy. 1 Two cases of double gallbladders managed laparoscopically have been reported previous... | Clinical case | biomedical | en | 0.999998 |
10036125 | Section title: INTRODUCTION Educational score: 4.058441162109375 Domain: biomedical Document type: Study Language: en In the 1960s, physicians of the Internal Clinic at the University of Erlangen, Germany, successfully performed endoscopic duodenoscopy, one of the earliest reports of that procedure. The first endoscopi... | Study | biomedical | en | 0.999997 |
10037783 | Section title: Cell Culture Educational score: 4.113435745239258 Domain: biomedical Document type: Study Language: en Mouse embryonic fibroblasts (MEFs), were prepared from day 13.5 embryos derived from wild-type (wt) and mTER −/− mice from different generations. The details are explained elsewhere . The first culture ... | Study | biomedical | en | 0.999996 |
10037784 | Section title: Cloning of Xenopus ADAR1.1 and ADAR1.2 Educational score: 4.273192405700684 Domain: biomedical Document type: Study Language: en Part of a Xenopus ADAR1 cDNA was isolated from an expression screen of a lambda Zap cDNA library for RNA-binding proteins . Sequence analysis identified this cDNA as a homologu... | Study | biomedical | en | 0.999998 |
10037785 | Section title: Cells and Cell Culture Educational score: 4.251619338989258 Domain: biomedical Document type: Study Language: en Two strains of normal human diploid myoblasts, both of satellite cell origin, were used in these studies. The strain designated 077 was a gift from R. Brown (Massachusetts General Hospital, Ch... | Study | biomedical | en | 0.999997 |
10037786 | Section title: Isolation of Primary Myoblasts, Cell Culture, and Immunohistochemistry Educational score: 4.147398948669434 Domain: biomedical Document type: Study Language: en Satellite cell–derived primary myoblasts were isolated from adult lower hindlimb muscle from 2–3-mo-old mice as described previously , with the ... | Study | biomedical | en | 0.999995 |
10037787 | Section title: Production of mAbs Educational score: 4.1464524269104 Domain: biomedical Document type: Study Language: en mAbs were obtained essentially according to the procedure of Köhler and Milstein . 50 μg of denatured recombinant human Ran was initially intraperitoneally administered with Freund's complete adjuva... | Study | biomedical | en | 0.999998 |
10037788 | Section title: Cells, Viruses, Antibodies, and Chemicals Educational score: 4.11646842956543 Domain: biomedical Document type: Study Language: en HeLa cells (human cervical epitheloid carcinoma) were grown as described earlier . TC7 cells (African green monkey kidney) and the TC7 clone TC7/MAP4/MTB–GFP stably transfect... | Study | biomedical | en | 0.999996 |
10037789 | Section title: Materials Educational score: 1.7201051712036133 Domain: biomedical Document type: Other Language: en N -(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza- s -indacene-3-pentanoyl)- d - erythro -sphingosine (C 5 -DMB-Cer) and 6-[ N -(7-nitrobenzo-2-oxa-1,3-diazol-4-yl)amino]caproyl- d - erythro- sphingosine (... | Other | biomedical | en | 0.714285 |
10037790 | Section title: Antibodies Educational score: 2.0734283924102783 Domain: biomedical Document type: Other Language: en Monoclonal antibody against rat GP2 has been described previously and was a gift from Dr. Anson Lowe (Stanford University). Monoclonal antibody KT3 against SV-40 large T antigen was a gift from Dr. Gerno... | Other | biomedical | en | 0.999994 |
10037791 | Section title: Anti–cytochrome c mAbs Educational score: 3.7252068519592285 Domain: biomedical Document type: Study Language: en The mouse anti–rat cytochrome c mAbs used in this study have been described . They were purified from ascites by affinity chromatography using rabbit cytochrome c–coupled Sepharose beads as i... | Study | biomedical | en | 0.999998 |
10037792 | Section title: Strains and Genetic Techniques Educational score: 3.2192299365997314 Domain: biomedical Document type: Study Language: en Yeast strains used in this study are listed in Table I . Culture and genetic analysis of yeast were performed by standard procedures . Semisynthetic lactate medium was prepared as des... | Study | biomedical | en | 0.999996 |
10037793 | Section title: Buffers and Reagents Educational score: 4.324542045593262 Domain: biomedical Document type: Study Language: en HB: 50 mM K-Hepes, pH 7.6, 1 mM MgCl 2 , 1 mM EGTA, 1 mM β-mercaptoethanol (β-ME) and protease inhibitor stock (1:200 final dilution; see below). HB100: HB plus 100 mM NaCl; HB200: HB plus 200 m... | Study | biomedical | en | 0.999995 |
10037794 | Section title: Cell Culture Educational score: 4.17070198059082 Domain: biomedical Document type: Study Language: en SV-40 transformed rat embryo cells (REF-2A) were maintained in DME containing 10% newborn calf serum in an atmosphere of 5% CO 2 and 95% air at 37°C. CHO cells were maintained in F12 medium containing 10... | Study | biomedical | en | 0.999995 |
10037795 | Section title: Materials Educational score: 1.5071810483932495 Domain: biomedical Document type: Other Language: en U46619 was from Cayman Chemical, thrombin, histone (subgroup f2b), monoclonal anti-myosin light chain (MLC) antibody, and fluorescein isothiocyanate (FITC)-phalloidin were from Sigma . Sp-5,6-DCl-cBIMPS a... | Other | biomedical | en | 0.999997 |
10037796 | Section title: Biological Materials Educational score: 3.225351095199585 Domain: biomedical Document type: Study Language: en HEK-293 cells (ATCC TIB202) were obtained from the American Type Culture Collection, and CCR5-transfected HEK-293 cells were kindly donated by Dr. J. Gutierrez (Dept. of Immunology and Oncology,... | Study | biomedical | en | 0.999995 |
10037797 | Section title: Cell Lines and Reagents Educational score: 4.043166160583496 Domain: biomedical Document type: Study Language: en Human recombinant IL-2, kindly provided by Dr. D. Bron (Institut Bordet, Brussels, Belgium), was used at 20 U/ml; IL-15 was obtained from Immunex and used at 200 ng/ml. Endotoxin-free affinit... | Study | biomedical | en | 0.999997 |
10037798 | Section title: Cells and Cell Lines Educational score: 4.270752906799316 Domain: biomedical Document type: Study Language: en DT40 cells deficient in Syk and those reconstituted with Syk have been previously established . Human M21 melanoma cells and established lymphoblastoid cell line (LCL) (JY) and lymphoid tumor ce... | Study | biomedical | en | 0.999997 |
10037799 | Section title: Cells, Antibodies, Chemicals, and Ig Fusion Proteins Educational score: 4.150020599365234 Domain: biomedical Document type: Study Language: en Human umbilical vein endothelial cells (HUVEC) were kindly donated by Dr. Sue Adams (Molecular Parasitology Group, University of Oxford) and used at the third pas... | Study | biomedical | en | 0.999997 |
10049938 | Section title: Lessons Learned from Mouse Tumor Models. Educational score: 4.672236442565918 Domain: biomedical Document type: Review Language: en Studies using adoptively transferred purified T cell subsets or in vivo depletion studies have firmly established an important role for tumor-specific CD8 + CTLs in antitumo... | Review | biomedical | en | 0.999996 |
10049939 | Section title: Cell Cultures. Educational score: 4.212894916534424 Domain: biomedical Document type: Study Language: en T cell, B cell, and melanoma cell lines were initiated from specimens derived from patient 1558, a 48-yr-old Caucasian male who had a primary cutaneous melanoma lesion resected from the right shoulder... | Study | biomedical | en | 0.999997 |
10049940 | Section title: Cell Lines, Media, and Reagents. Educational score: 4.219888687133789 Domain: biomedical Document type: Study Language: en The EBV-transformed B (EBV-B) cell lines and tumor cell line MZ2-MEL.43 were cultured in IMDM ( GIBCO BRL ) supplemented with 10% FCS ( GIBCO BRL ), 0.24 mM l -asparagine, 0.55 mM l ... | Study | biomedical | en | 0.999997 |
10049941 | Section title: Mice. Educational score: 4.0361785888671875 Domain: biomedical Document type: Study Language: en C57BL/6 mice were purchased from IFFA Credo. Mice homozygous for lpr mutation ( lpr ) and lacking the Fas (CD95) molecule were obtained from Harlan. Mice homozygous for perforin gene disruption (P 0/0 ) compl... | Study | biomedical | en | 0.999997 |
10049942 | Section title: cDNA Library Construction. Educational score: 3.944190502166748 Domain: biomedical Document type: Study Language: en The expression cDNA library was prepared in VR1012 plasmid (Vical Inc.) using RNA extracted from IL-2–activated polyclonal NK cells as described ( 7 , 8 ), and was divided into 10 fraction... | Study | biomedical | en | 0.999996 |
10049943 | Section title: Mice and Antibodies. Educational score: 2.1173245906829834 Domain: biomedical Document type: Study Language: en BALB/cJ and C57BL/6 mice were obtained from The Jackson Laboratory . Antibodies to CRT and protein disulfide isomerase (PDI) were purchased from Affinity BioReagents , and antibodies to gp96 we... | Study | biomedical | en | 0.999997 |
10049944 | Section title: Mice. Educational score: 4.062753677368164 Domain: biomedical Document type: Study Language: en BALB/c mice were purchased from Bomholtgaard Breeding & Research Centre, Denmark. IL-4-deficient mice (IL-4 −/− ) were generated by targeted disruption of the IL-4 gene in embryonic stem cells derived from BAL... | Study | biomedical | en | 0.999998 |
10049945 | Section title: Experimental Animals and Cell Lines. Educational score: 3.9640896320343018 Domain: biomedical Document type: Study Language: en BALB/cByJ, CByD2F1, C3H/J, and C57BL/6j mice were purchased from The Jackson Laboratory . Nude mice (nu/nu) were obtained from The National Cancer Institute. 6–18-wk-old male mi... | Study | biomedical | en | 0.999994 |
10049946 | Section title: Generation and Stimulation of DCs. Educational score: 4.129424571990967 Domain: biomedical Document type: Study Language: en To generate immature DCs, peripheral blood monocytes purified by centrifugal elutriation were cultured in RPMI-1640 supplemented with 10% FCS (Hyclone), 50 ng/ml GM-CSF, and 1,000 ... | Study | biomedical | en | 0.999997 |
10049947 | Section title: Immunogens. Educational score: 4.207452774047852 Domain: biomedical Document type: Study Language: en For the production of isoform-specific polyclonal antisera directed against Nramp2, rabbits were immunized with fusion proteins containing glutathione S-transferase (GST) fused to a peptide segment deriv... | Study | biomedical | en | 0.999997 |
10049948 | Section title: Materials. Educational score: 3.5994789600372314 Domain: biomedical Document type: Study Language: en Human recombinant IL-1β, TNFα, and IFNγ were obtained from Endogen . Phorbol-12 myristate 13-acetate (PMA) and polymyxin B were purchased from Sigma Chemical Co. Human recombinant CD40L (rCD40L) was gene... | Study | biomedical | en | 0.999996 |
10049949 | Section title: Reagents. Educational score: 3.8620615005493164 Domain: biomedical Document type: Study Language: en The protein G–Sepharose column–purified antibodies anti–λ1 L chain (Ls136) and FDC-M2 were provided by Garnett Kelsoe (Duke University, Durham, NC) and Marie Kosco-Vilbois (Serono Pharmaceutical Research ... | Study | biomedical | en | 0.999996 |
10049950 | Section title: Cell Preparations. Educational score: 4.07581901550293 Domain: biomedical Document type: Study Language: en Ag-specific CD4 + human T cell lines were isolated as described ( 13 ). PBMCs were separated into CD4 + T cells, CD8 + T cells, CD19 + B lymphocytes, and CD14 + monocytes using immunomagnetic beads... | Study | biomedical | en | 0.999997 |
10049951 | Section title: T Cell Epitope Prediction. Educational score: 4.134321212768555 Domain: biomedical Document type: Study Language: en TEPITOPE, a new T cell epitope prediction software, is a Windows™ application that enables the identification of (a) class II ligands binding in a promiscuous or allele-specific mode, and ... | Study | biomedical | en | 0.999996 |
10049952 | Section title: mAbs. Educational score: 3.3854706287384033 Domain: biomedical Document type: Study Language: en The mouse mAbs used in these studies were as follows: OX6 (anti–rat class II MHC; reference 15 ), OX7 (anti–rat Thy-1; reference 16 ), OX8 (anti–rat CD8; reference 17 ), and OX12 (anti–rat κ chain; reference ... | Study | biomedical | en | 0.999998 |
10051512 | Section title: Spark fluorescence and the underlying time course of SR Ca 2+ release. Educational score: 4.430673599243164 Domain: biomedical Document type: Study Language: en Before discussing the number of channels responsible for generating a Ca 2+ spark, it is important to first consider what information the spark ... | Study | biomedical | en | 0.999998 |
10051514 | Section title: How Big Is a Spark? Educational score: 4.099489212036133 Domain: biomedical Document type: Study Language: en Measured spark amplitude varies widely from the lower limits of detection (defined by noise, usually ∼0.3 U F 0 ) to the highest values recorded, which in our experience with cut fiber segments u... | Study | biomedical | en | 0.999997 |
10051516 | Section title: introduction Educational score: 4.177549362182617 Domain: biomedical Document type: Other Language: en Voltage-dependent ion channels respond to changes in the electric field across the cell membrane by undergoing conformational changes that open and close an ion-permeable pore. The voltage dependence of... | Other | biomedical | en | 0.999997 |
10051517 | Section title: introduction Educational score: 4.442716121673584 Domain: biomedical Document type: Study Language: en With the determination of the high-resolution structure of the KcsA K + channel from Streptomyces lividans , structure–function studies of ion channel proteins can henceforth include structure. This adv... | Study | biomedical | en | 0.999996 |
10051518 | Section title: introduction Educational score: 4.308370113372803 Domain: biomedical Document type: Study Language: en Large conductance Ca 2+ -activated K + channels (BK channels) 1 play an important role in regulating the excitability of nerve, muscle, and other cells by stabilizing the cell membrane at negative poten... | Study | biomedical | en | 0.999998 |
10051519 | Section title: introduction Educational score: 3.9957354068756104 Domain: biomedical Document type: Other Language: en Maintenance and regulation of cell volume are fundamental physiological processes. In response to swelling, cells activate anion and cation channels that allow the passive loss of inorganic ions and or... | Other | biomedical | en | 0.999994 |
10051520 | Section title: introduction Educational score: 4.249289035797119 Domain: biomedical Document type: Study Language: en ClC proteins represent a class of voltage-dependent Cl − channels with several members involved in hereditary human diseases. The Cl − channel prototype ClC-0 from Torpedo is a dimeric protein behaving ... | Study | biomedical | en | 0.999997 |
10051521 | Section title: introduction Educational score: 4.791410446166992 Domain: biomedical Document type: Study Language: en The intracellular signal that triggers the contraction of cardiac muscle is a transient rise in intracellular free calcium. The majority of this calcium (50–95%, depending upon species and conditions) i... | Study | biomedical | en | 0.999996 |
10051522 | Section title: introduction Educational score: 4.829248905181885 Domain: biomedical Document type: Study Language: en The visual cycle is the series of reactions occurring in the eye through which visual pigment proceeds from photoactivation to regeneration. Absorption of a photon by the pigment of a photoreceptor trig... | Study | biomedical | en | 0.999998 |
10063307 | Section title: INTRODUCTION Educational score: 3.1745996475219727 Domain: biomedical Document type: Study Language: en Although the development mechanism of chronic viral hepatitis B(CVH-B), which is the most important etiologic factor in liver cirrhosis(LC) and hepatocellular carcinoma (HCC) in Korea, has not been ful... | Study | biomedical | en | 0.999997 |
10063308 | Section title: INTRODUCTION Educational score: 4.26658821105957 Domain: biomedical Document type: Study Language: en Helicobacter pylori infection is now recognized as the major cause of chronic gastritis throughout the world 1 – 3 ) . A fraction of infected persons developed peptic ulcer disease 4 ) or gastric cancer ... | Study | biomedical | en | 0.999997 |
10063309 | Section title: INTRODUCTION Educational score: 4.557106018066406 Domain: biomedical Document type: Study Language: en Helicobacter pylori (H. pylori) infection is now recognized as the cause of type B gastritis, as a critical factor in the development and the recurrence of duodenal ulcer disease, and as an essential co... | Study | biomedical | en | 0.999995 |
10063310 | Section title: INTRODUCTION Educational score: 4.471651554107666 Domain: biomedical Document type: Study Language: en Nitric oxide (NO), produced by the conversion of the terminal guanidino nitrogen atoms from L-arginine, accounts for many of the biological properties of endothelium-derived relaxing factor (EDRF) 1 ) .... | Study | biomedical | en | 0.999997 |
10063311 | Section title: INTRODUCTION Educational score: 3.9577443599700928 Domain: biomedical Document type: Review Language: en Although the prevalence rate of pulmonary tuberculosis is decreasing due to the National Tuberculosis Control Programme, drug resistant pulmonary tuberculosis has been a longstanding public health pro... | Review | biomedical | en | 0.999995 |
10063312 | Section title: INTRODUCTION Educational score: 4.235065460205078 Domain: biomedical Document type: Study Language: en Diabetic nephropathy is the leading cause of end-stage renal disease 1 ) . In addition, the development of diabetic nephropathy has a dramatic impact on the morbidity and mortality in diabetic patients ... | Study | biomedical | en | 0.999994 |
10063313 | Section title: INTRODUCTION Educational score: 4.596819877624512 Domain: biomedical Document type: Study Language: en Cisplatin is one of the most active anticancer agents for the treatment of lung cancer and the cisplatin-based chemotherapeutic regimens have produced a statistically significant and clinically relevant... | Study | biomedical | en | 0.999998 |
10063314 | Section title: INTRODUCTION Educational score: 4.268034934997559 Domain: biomedical Document type: Study Language: en It is clear that in human cancer, including lung cancer, deregulation of cell cycle progression is a hallmark of neoplastic transformation and that genes involved in G1/S transition of the cell cycle ar... | Study | biomedical | en | 0.999996 |
10063315 | Section title: INTRODUCTION Educational score: 4.275685787200928 Domain: biomedical Document type: Study Language: en Rheumatoid arthritis is characterized by hyperplasia of synovial lining cells, excessive infiltration of mononuclear cells and extensive destruction of the articular cartilage 1 ) , and this abnormal pr... | Study | biomedical | en | 0.999997 |
10063316 | Section title: INTRODUCTION Educational score: 3.457967519760132 Domain: biomedical Document type: Study Language: en Behcet disease is characterized by recurrent painful oral ulcer and genital ulcer, skin diseases and uveitis. It may involve cardiovascular, gastrointestinal and central nervous system, as well as joint... | Study | biomedical | en | 0.999995 |
10063317 | Section title: INTRODUCTION Educational score: 3.911609411239624 Domain: biomedical Document type: Clinical case Language: en Pylephlebitis is defined as septic thrombophlebitis of the portal vein or one of its tributaries, usually secondary to suppuration either in the region drained by the portal venous system or in ... | Clinical case | biomedical | en | 0.999997 |
10063318 | Section title: INTRODUCTION Educational score: 3.6276779174804688 Domain: biomedical Document type: Study Language: en Diabetic patients, who consume Vacor (N-3-pyridylmethyl-N’-p-nitrophenylurea, MW272), experience microangiopathy causing retinopathy and nephropathy 1 ) . The width of the capillary basement membrane i... | Study | biomedical | en | 0.999997 |
10063319 | Section title: INTRODUCTION Educational score: 3.701921224594116 Domain: biomedical Document type: Review Language: en Low-dose Methotrexate (MTX) (5.0–20.0 mg/week), initially widely used to treat severe psoriasis and psoriatic arthritis, is one of the effective therapies for rheumatoid arthritis (RA) 1 ) . Its tolera... | Review | biomedical | en | 0.999997 |
10063320 | Section title: INTRODUCTION Educational score: 3.4658641815185547 Domain: biomedical Document type: Other Language: en Leiomyosarcomas are uncommon malignant tumors that originate in smooth muscle, usually in the gastrointestinal tract, the retroperitoneum and the genitourinary tract 1 ) . Metastatic tumors in the omen... | Other | biomedical | en | 0.999997 |
10063321 | Section title: INTRODUCTION Educational score: 4.07218599319458 Domain: biomedical Document type: Review Language: en Cytomegalovirus (CMV) disease is a major cause of morbidity and mortality in immunocompromised patients, particularly recipients of solid organ or bone marrow allograft, and patients with the acquired i... | Review | biomedical | en | 0.999997 |
10063322 | Section title: INTRODUCTION Educational score: 4.268415927886963 Domain: biomedical Document type: Clinical case Language: en Juvenile dermatomyositis (DM) is a multisystem disease characterized by nonsuppurative inflammation of striated muscle, skin and the gastrointestinal tract, and also characterized early in its c... | Clinical case | biomedical | en | 0.999996 |
10075972 | Section title: Mice. Educational score: 4.050711631774902 Domain: biomedical Document type: Study Language: en B6, transporter associated with antigen processing (TAP)1 −/− ( 29 ), β 2 m −/− ( 30 , 31 ), and TAP1/β 2 m −/− ( 32 ) mice of the H-2 b haplotype were bred at the Microbiology and Tumor Biology Center, Karoli... | Study | biomedical | en | 0.999997 |
10075973 | Section title: Cell Lines. Educational score: 4.287715911865234 Domain: biomedical Document type: Study Language: en The human melanoma cell lines NA8-MEL (provided by Dr. F. Jotereau, Institut National de la Santé et de la Recherche Médicale, Nantes, France) and SK23-MEL were cultured in RPMI medium ( GIBCO BRL ) supp... | Study | biomedical | en | 0.999997 |
10075974 | Section title: Special Reagents. Educational score: 2.232832193374634 Domain: biomedical Document type: Study Language: en TAQ polymerase was from Boehringer-Mannheim , radiolabeled γ-[ 32 P]ATP was from New England Nuclear , PMA, dimethylacetamide, DMSO, and polymyxin B sulfate were from Sigma Chemical Co. , human rec... | Study | biomedical | en | 0.999997 |
10075975 | Section title: Mice. Educational score: 1.7254506349563599 Domain: biomedical Document type: Other Language: en Female C57BL/6 mice 6–8 wk of age were purchased from Harlan Sprague Dawley, Inc. Female C57BL/6 (nu/nu) mice were purchased from Taconic Farms, Inc. IL-4 and IL-10 gene–disrupted C57BL/6 mice were purchased ... | Other | biomedical | en | 0.999996 |
10075976 | Section title: Hemorrhagic Shock Protocol. Educational score: 0.9134503602981567 Domain: biomedical Document type: Other Language: en This study was performed in accordance with the National Institute of Health guidelines for the use of experimental animals, and all animal protocols have been approved by the Institutio... | Other | biomedical | en | 0.999997 |
10075977 | Section title: Protease Inhibitor. Educational score: 3.8506786823272705 Domain: biomedical Document type: Study Language: en KD-IX-73-4 (HO-NH-CO-CH 2 -CH(CH 2 -CH(CH 3 ) 2 )-CO-Nal-Ala-NH 2 ) was a gift from Dr. T.K. Kishimoto ( Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT). 0.5 mg of the protease inhib... | Study | biomedical | en | 0.999996 |
10075978 | Section title: Mice. Educational score: 1.81117844581604 Domain: biomedical Document type: Other Language: en Male Balb/cByJ mice at 8–14 wk of age were obtained from The Jackson Laboratory . Mice were housed at least 1 wk before experimentation. Mice were cared for and handled at all times in accordance with the Natio... | Other | biomedical | en | 0.999997 |
10075979 | Section title: Mice. Educational score: 3.179295063018799 Domain: biomedical Document type: Study Language: en p53 gene–deficient, Bcl-2 transgenic (Tg), DO.11.10 OVA TCR-α/β Tg, and P14 TCR-α/β Tg mice have been described previously ( 30 – 33 ). BALB/c mice were purchased from Taconic Farms. All mice were kept at the ... | Study | biomedical | en | 0.999998 |
10075980 | Section title: Mice. Educational score: 2.7509965896606445 Domain: biomedical Document type: Study Language: en SJL/J and C57BL/6 mice were obtained from the National Cancer Institute. Breeding pairs of C57BL/6 IL-12 −/− (N6) and C57BL/6 IFN-γ −/− were originally provided by J. Magram (Hoffman-La Roche, Nutley, NJ) and... | Study | biomedical | en | 0.999996 |
10075981 | Section title: Reagents. Educational score: 1.545371651649475 Domain: biomedical Document type: Other Language: en Type I collagen solution extracted from porcine skin (Cellmatrix I-A) was purchased from Nitta Gelatin Co. EC growth supplement (ECGS) and porcine heparin were purchased from Collaborative Research and Nak... | Other | biomedical | en | 0.999997 |
10075982 | Section title: Animals Used in the Study. Educational score: 3.874772548675537 Domain: biomedical Document type: Study Language: en Seven SIV-infected and one uninfected rhesus macaque ( Macaca mulatta ) were used (see Table I ). Six infected and one uninfected animal were given OKT8F (gift from R. Knowles, R.W. Johnso... | Study | biomedical | en | 0.999999 |
10075983 | Section title: Cell Culture and Treatments. Educational score: 4.078702449798584 Domain: biomedical Document type: Study Language: en Mouse embryonic fibroblasts (MEFs) derived from RelA-deficient mice ( 9 ) were cultivated in DMEM supplemented with 10% calf serum and antibiotics. MEFs were stimulated in the presence o... | Study | biomedical | en | 0.999999 |
10075984 | Section title: Mice. Educational score: 4.090842247009277 Domain: biomedical Document type: Study Language: en SJL/J and CSJLF1/J mice were obtained from The Jackson Laboratory . BALB/cAnN mice were obtained from Taconic Farms, Inc. hIL-10Tg mice were constructed using a hIL-10 cDNA sequence regulated by a class II MHC... | Study | biomedical | en | 0.999997 |
10075985 | Section title: Animals and Treatments. Educational score: 4.040351867675781 Domain: biomedical Document type: Study Language: en Homozygous CD7-deficient mice ( 12 ) were backcrossed five generations onto the C57BL/6 background. C57BL/6 mice were obtained from The Jackson Laboratory . 10–12-wk-old sex-matched mice were... | Study | biomedical | en | 0.999997 |
10085282 | Section title: Bni1p, a Formin, Functions in Kip3p-dependent Spindle Positioning and Movement Educational score: 3.8220813274383545 Domain: biomedical Document type: Study Language: en Both groups found that bni1 mutants had defects in spindle positioning, using different approaches. The nucleus did not migrate to the ... | Study | biomedical | en | 0.999997 |
10085283 | Section title: Cell Culture Educational score: 4.16635799407959 Domain: biomedical Document type: Study Language: en Indian muntjac (DM) cells were grown in DME + 10% FCS ( GIBCO BRL ) and plated on glass-bottom microwell dishes coated with poly- d -lysine (Mattek). Cells were bead loaded using 100-μm glass beads ( Sig... | Study | biomedical | en | 0.999998 |
10085284 | Section title: Antibodies and Cell Lines Educational score: 4.208955764770508 Domain: biomedical Document type: Study Language: en The rabbit polyclonal antiserum against the COOH-terminal peptide of p27 BBP/eIF6 (NH 2 -CTIATSMRDSLIDSLT-COOH) was tested for its specificity by Western blotting and immunoprecipitation bo... | Study | biomedical | en | 0.999995 |
10085285 | Section title: Subcellular Fractionation Educational score: 3.8811216354370117 Domain: biomedical Document type: Study Language: en The yeast strains Saccharomyces uvarum , considered a strain of Saccharomyces cerevisiae , or S . cerevisiae were used throughout the procedure. Enriched nuclei, NEs and heparin-extracted ... | Study | biomedical | en | 0.999997 |
10085286 | Section title: Construction of the Calreticulin Knockout Vector Educational score: 4.289483547210693 Domain: biomedical Document type: Study Language: en The plasmid pNTK (containing the PGK neomycin cassette and PGK thymidine kinase) was used to generate the knockout vector. The calreticulin promoter was cloned previo... | Study | biomedical | en | 0.999994 |
10085287 | Section title: Immunofluorescence Microscopy of Fixed Normal Rat Kidney (NRK) Cells Educational score: 4.31304931640625 Domain: biomedical Document type: Study Language: en NRK cells were maintained in DME containing 5 g glucose per liter, 10% FCS, and antibiotics in a humidified 5% CO 2 incubator. For transfection wit... | Study | biomedical | en | 0.999997 |
10085288 | Section title: Culture of Sympathetic Neurons Educational score: 4.058577537536621 Domain: biomedical Document type: Study Language: en Sympathetic neurons from newborn rat cervical superior ganglia were cultured as previously described . For electron microscopy studies, neurons were fixed with 1% glutaraldehyde in PBS... | Study | biomedical | en | 0.999997 |
10085289 | Section title: Antibodies Educational score: 3.917039632797241 Domain: biomedical Document type: Study Language: en Rabbit pAbs were raised against amino acids 1–21 of human Bax , amino acids 11–30 of human Bax (Bax N-20 sc-493; Santa Cruz Biotechnology ), amino acids 43–61 of murine Bax . Note that the antibodies rais... | Study | biomedical | en | 0.999996 |
10085290 | Section title: Cell Culture, Drug Treatment, and Analysis of Apoptosis Educational score: 4.1758341789245605 Domain: biomedical Document type: Study Language: en The human Jurkat T-leukemic cell line was grown in RPMI 1640 medium supplemented with 10% fetal bovine serum and 2 mM l -glutamine ( GIBCO BRL ). The human CE... | Study | biomedical | en | 0.999998 |
10085291 | Section title: Cell Lines and Cell Production Educational score: 4.140338897705078 Domain: biomedical Document type: Study Language: en 697 human lymphoblastoid cells stably infected with a retroviral expression construct containing bcl-2 cDNA or a control neomycin resistance gene were used in these studies. The cells ... | Study | biomedical | en | 0.999997 |
10085292 | Section title: Culture Conditions and Strains Educational score: 3.891291856765747 Domain: biomedical Document type: Study Language: en Basic methods of C . elegans culture and handling were as previously described . Strains carrying the following mutations were used: unc-32(e189) , qC1 dpy-19 glp-1(q339) , him-3 , zyg... | Study | biomedical | en | 0.999997 |
10085293 | Section title: Microbial Techniques Educational score: 3.961561441421509 Domain: biomedical Document type: Study Language: en Media and genetic techniques were as described . The mating pheromone, α-factor, was added to log phase cultures at 6 μg/ml. Latrunculin-A (Lat-A) (from P. Crews, University of California, Santa... | Study | biomedical | en | 0.999996 |