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10.3390/atmos13020266
Xuejun Liu
Dias, M.
Gomes, B.
Cervantes, R.
Pena, P.
Viegas, S.
Viegas, C.
Open AccessReview
Microbial Occupational Exposure Assessments in Sawmills—A Review
Marta Dias
1,2,3 ,
Bianca Gomes
Renata Cervantes
Pedro Pena
Susana Viegas
1,2,3 and
Carla Viegas
NOVA National School of Public Health, Public Health Research Centre, Universidade Nova de Lisboa, 1099-085 Lisbon, Portugal
Comprehensive Health Research Center (CHRC), NOVA Medical School, Universidade NOVA de Lisboa, 1169-056 Lisbon, Portugal
H&TRC—Health & Technology Research Center, ESTeSL—Escola Superior de Tecnologia e Saúde, Instituto Politécnico de Lisboa, 1990-096 Lisbon, Portugal
Atmosphere 2022, 13(2), 266; https://doi.org/10.3390/atmos13020266
Received: 19 December 2021 / Revised: 18 January 2022 / Accepted: 28 January 2022 / Published: 4 February 2022
(This article belongs to the Special Issue Occupational Exposure Biological Agents: Focus on a Growing Concern)
The composition of airborne microflora in sawmills may vary to a great degree depending on the kind of timber being processed and the technology of production being used. Cases of allergy alveolitis and asthma have been reported in woodworkers who were exposed to wood dust largely infected with microorganisms. The aim of this review article is to identify studies where the microbial occupational exposure assessment was performed in sawmills and the characteristics of the contamination found, as well as to identify which sampling methods and assays were applied. This study reports the search of available data published regarding microbial occupational exposure assessment in environmental samples from sawmills, following the Preferred Reporting Items for Systematic Reviews (PRISMA) methodology. The most used sampling method was air sampling, impaction being the most common method. Regarding analytical procedures for microbial characterization, morphological identification of fungi and bacteria was the most frequent approach. Screening for fungal susceptibility to azoles was performed in two studies and four studies applied molecular tools. Regarding microbial contamination, high fungal levels were frequent, as well as high bacteria levels. Fungal identification evidenced Penicillium as the most frequent genera followed by Aspergillus sp. Mycotoxins were not assessed in any of the analyzed studies. Microbial occupational exposure assessment in sawmills is crucial to allow this risk characterization and management.
occupational exposure; exposure assessment; sawmills; woodworkers; azole resistance; microbial contamination
Globally, the sawmill market is primarily driven by rising construction demand, which accounts for roughly 73.48 percent of total downstream consumption of sawmill in the world. Softwood and hardwood are the two types of sawmill raw materials. Its downstream use is diverse, and recently, building and furniture have gained prominence in a variety of sawmill areas [1].
Workers in sawmill industry may be exposed to allergic, carcinogenic, and immunotoxic agents, comprising wood derivatives (e.g., terpenes, resin acids) as well microorganisms that grow on timber (bacteria and fungi) and their products (endotoxins and mycotoxins) known as potential causative agents of health effects [2,3,4,5,6,7,8]. Exposure can result in decreased lung function, bronchial hyperresponsiveness, and a variety of disorders such as organic dust toxic syndrome (ODTS), allergic alveolitis, asthma, chronic bronchitis, rhinitis, mucous membrane irritation (MMI), contact dermatitis, and nasal cancer [9,10,11,12,13,14,15,16,17,18]. The majority of the negative effects generated by microorganisms linked with wood dust have an immunological basis. The most well-known are those produced by fungi, which may thrive in the right conditions on stored wood products (planks, chips) as a secondary wood infection [18].
Inhaling large amounts of spores and mycelial fragments of Aspergillus sp., Penicillium sp., Rhizopus sp., Paecilomyces sp., Mucor sp. and other fungi can result in a strong antibody response and respiratory disorders, most commonly allergic alveolitis (wood trimmer's disease) or organic dust toxic syndrome in exposed workers [18,19,20,21,22,23,24,25,26,27]. Cases of allergy alveolitis and asthma have been reported in woodworkers who were exposed to wood dust largely infected with fungi during logging, debarking, and sawing tasks [18].
The composition of airborne microflora in sawmills may vary to a great degree depending on the kind of timber being processed and the technology of production [8,15,16,28]. In wood processing, preservation, and maintenance azole fungicides are used for the protection of spruce and pine fields [28,29]. To protect wood from wood-destroying basidiomycete fungus, sawmills, particularly those working with resinous timbers, typically use azole fungicides. This fungus can induce deterioration or blueing of wood, rendering it useless [28,30]. Propiconazole and tebuconazole are the most common azole compounds found in sawmills. In fact, these two compounds are among the five 14-demethylase inhibitors (DMIs) linked to clinical azoles and contributing to the rise in azole antifungal resistance [28,30,31,32]. Furthermore, Aspergillus section Fumigati azole antifungal resistance was already reported in this environment [28,29].
Portugal's social and economic history is inextricably related to the products of the forest, where national economic organizations are world leaders in the production and trading of forest products [33]. Regarding the sawmill industry in Portugal, 2250 million euros were made with exportations in 2020, there were 8700 companies reported in the wood industry in 2019 and, consequently, about 56,000 workers account for this sector workforce [34].
Due to the lack of studies in Portuguese sawmills this study aimed to perform a systematic review to provide a broad overview of the state of art in the developed subject, describing the microbiological contamination reported in previous studies developed in sawmills and indicating which parameters and methods were applied to perform the microbial occupational exposure assessment in this setting. These study results will contribute to a sampling and analyses protocol proposal aiming to assess the occupational exposure to microbial contamination is this specific occupational environment.
2.1. Registration
The Preferred Reporting Items for Systematic Reviews (PRISMA) checklist [35] was completed (Supplementary Materials Table S1).
2.2. Search Strategy, Inclusion and Exclusion Criteria
This study reports the search of available data published between the period of 1 January 2000 and 30 September 2021. The search terms aimed to identify studies in microbial occupational exposure assessments, selecting studies on sawmills that included the terms "occupational exposure", "sawmills", with English as the chosen language. The databases chosen were PubMed, Scopus, Web of Science (WoS) and other sources, following the PRISMA methodology. This search strategy identified 441 papers in all databases. Articles that did not fulfil the inclusion criteria were not subjected to additional review (but some of them were used for introduction and discussion sections) (Table 1).
2.3. Studies Selection and Data Extraction
The selection of the articles was performed through Rayyan, which is a free web-tool that greatly speeds up the process of screening and selecting papers for academics working on systematic reviews, in three rounds by three investigators (MD, BG, and RC). The first round consisted of a screening of all titles to exclude papers that were duplicated or unrelated to the subject, and then the included added to Rayyan for further analysis. The second round consisted of a screening of all abstracts. In the third round, the full texts of all potentially relevant studies were reviewed considering the inclusion and exclusion criteria. Potential divergences in the selection of the study were discussed and ultimately resolved by the remaining investigators (CV and SV). Data extraction was performed by two investigators (BG and RG) and reviewed by another (MD). The following information was manually extracted: (1) Database, (2) Title, (3) Country, (4) Occupational Environment, (5) Sampling Methods, (6) Analytical Methods, (7) Main Findings, and (8) References.
2.4. Quality Assessment
The assessment of the risk of bias was performed by two investigators (MD and CV). Within each study, we evaluated the risk of bias across three parameters divided as key criteria (Sampling Methods, Analytical Methods) and other criteria (data about metabolites). The risk of bias for each parameter was evaluated as "low", "medium", "high", or "not applicable". The studies for which all the key criteria and most of the other criteria are characterized as "high" were excluded.
The flow diagram for selecting studies is shown in Figure 1. The initial database search yielded 441 studies, from which 133 abstracts were examined and 40 full texts were evaluated for eligibility. A total of 18 studies were rejected after examining the inclusion and exclusion criteria, primarily because they were related to biological samples collected from the sawmill workers. A total of 23 papers on microbial occupational exposure were chosen.
Characteristics and Data Obtained in the Selected Studies
Table 2 describes the main characteristics from the selected studies. From the selected studies (N = 23), 15 were conducted in the Europe, namely 5 in Norway [29,36,37,38,39], 4 in Poland [8,40,41,42], 2 in Switzerland [43,44], 2 in Croatia [45,46], 1 in Finland [47], 1 in Italy [48], and 1 in France [30]. Five studies from Canada [49,50,51,52,53], 1 from Korea [54], and 1 from Iran [55] were also analyzed. The majority of studies (15 out of 23–65.2%) analyzed environmental samples from small and medium size sawmills [18,28,36,37,38,39,40,41,42,43,44,45,46,47,48,49,51,52], 2 studies (8.7%) were performed in industrial sawmills [29,39], 2 studies (8.7%) in plywood hardwood processing companies [53], 1 (4.4%) in a manufacturing industry [51], 1 (4.4%) in carpentries [48], 1 (4.4%) in pellet production facilities [42], and 1 (4.4%) in a furniture factory [41].
The most used sampling method was air sampling (19 out of 23–82.6%) [18,29,36,37,38,39,40,43,44,46,47,49,50,51,52,53,54,55]. Several studies used more than one active sampling method (8 out of 23–34.8%). Air collection through impaction was used in 16 studies (69.6%) [8,40,42,43,44,45,46,49,50,52,53,54,55], followed by filter air sampling in 11 studies (47.8%) [28,29,37,38,41,44,46,48,50,53,54], while 5 studies (21.7%) used the impingement method [29,47,49,50,52].
Passive methods were exclusively performed in 5 papers (21.7%) [28,40,41,47,53]. Dust samples collection was the most frequent methodology applied (N = 3) [28,41,53], one study collected wood samples [40] and the other performed surface samples [53].
Concerning analytical procedures for microbial characterization, 13 studies (56.5%) referred to fungi [28,29,37,38,39,41,45,46,47,48,50,51,53], 1 (4.4%) referred only to bacteria [40], while 9 (39.1%) encompassed fungi and bacteria [8,42,43,44,49,50,52,54,55]. Morphological identification was the most frequent approach. Fungal identification was accomplished through macroscopic and microscopic examination in 16 studies (69.6%) [8,28,29,41,42,43,44,45,46,49,50,51,52,53,54,55]. Regarding bacterial identification, 5 studies (21.7%) used biochemical tests [8,40,42,50,55].
Screening for fungal susceptibility to azoles was performed in 2 studies (8.7%). For the screening of A. fumigatus azole resistance, 1 study (4.4%) used the EUCAST methods [53] and the other used both EUCAST and E-test methods [28].
Molecular tools were applied in 4 studies (17.4%). All performed DNA sequencing [28,29,39,42,55]. High fungal levels were frequent in 6 studies (26.1%) [8,44,45,46,50,54], as well as high bacteria levels in 4 studies (17.4%) [8,43,50,54]. Fungal identification evidence Penicillium as the most frequent genera [41,43,46,47,49,50,52,53,55]. Aspergillus sp. was also recurrent in 4 studies (17.4%) [8,29,42,46]. From all the sampling sites, 3 studies (13%) reported the sorting and green department as having the highest levels of fungal fragments [36,37,38]. Other working sites were also associated with potential microbial exposure as follows: saw departments [36,39], dry timber departments [37], and debarking site [49]. In fact, 7 studies (30.4%) report airborne fungi as potential agents for occupational health effects [8,41,42,44,45,46,50], as well as bacteria in 2 studies (8.7%) [40,56]. In what concerns mycological diversity, 3 studies (13%) report fungal bioaerosols variation between different indoor locations [39,49,51] and 4 studies (17.4%) evidence a significant influence of seasons in fungal aerosol composition [36,38,39,49].
It is well known that sawmill workers are exposed to wood dust and multiple wood-associated chemicals and microbiota, including fungi [1,2,3,4,30,31]. Fungi and Gram-negative bacteria are major contaminants of wood dust, especially in hot and humid areas. Occupational inhalation exposure to wood dust and its associated bioaerosols (composed by fungi, bacteria, endotoxins, mycotoxins, and much more) has been associated with adverse respiratory effects [5,6,7,8,9]. Health outcomes associated with the inhalation of wood dust have been reported in several studies [5,9,11,12,13,15,16,17,18,19,20] as well as a significant association between inhalation of wood dust and an increased prevalence of respiratory symptoms [13,21,22,23,28] and decreased lung functional capacity [55]. Considering the papers included in this review, most of them (21 out of 23) used air as an environmental matrix, impaction being the most frequent sampling method used (15 out of 23). This sampling approach relies solely on culture-based methods, which can have advantages and disadvantages. The inflammatory and/or cytotoxic potential can affect the microorganism viability [56,57] which makes this method beneficial since it allows us to rely on the microbial composition to draw conclusions regarding the inflammatory potential variation [57,58]. In impaction sampling devices, a specific flow rate (depending on the type of environment) is defined to collect particles [59] by using its inertia to drive deposition on a collection media by promoting particle separation through an air stream [60]. However, since it only allows to evaluate culturable microorganisms, the microbial load can be underestimated, due to the high velocity of the air flow that may result in microorganisms' cell damage [61,62]. Moreover, it is important to highlight that indoor air is not homogeneous in space or time, it can always change depending on the type and intensity of the activity developed in that space [63]. Therefore, the sampling time must be adequate to the environment in study and work tasks being developed. For example when using high volume samplers in highly contaminated areas, it is crucial to employ short sampling intervals and lower flow rates for airborne fungal sampling [64]. Nevertheless, active sampling methods, namely impaction devices, have already proved to be very useful in the characterization of occupational exposure to fungi in several studies, by presenting the most diversified fungal contamination in comparison with all sampling methods applied [28,51,61,65,66].
Passive sampling methods were also used, even if in a smaller number (3 out of 23 papers, including studies with one or more sampling methods). There is evidence that ventilation, building design, environmental features [67], or water infiltrations and damage [68], geographical location [69], as well as the type of task developed in each working site [36,49] can alter fungi and bacteria found indoors. Different working sites were identified with potential for microbial exposure namely the ones that include sawing and drying, mainly because the cells in hardwood are firmly bonded, and kiln drying renders them less elastic, resulting in cell breakage and tiny airborne dust [70,71].
With so many factors impacting microbial contamination indoors, passive sampling approaches are anticipated to be more reliable than active sampling methods since they can collect contamination over a longer period of time, thus covering all expected fluctuations [72,73]. The passive sampling method used in all three studies was the collection of wood dust, which both acute and chronic exposures may serve as a sensitizer and irritant on the human body, mostly affecting the respiratory system and skin [56].
Several researchers [67,73,74,75,76,77,78] have begun to collect and analyze from indoor environments a similar matrix (settled dust) as part of their microbial contamination exposure assessments. Settled dust reservoirs have been described as having the ability to anticipate microbial levels in indoor air, as well as being more repeatable than active sampling approaches [67]. Furthermore, it has been documented as an environmental support for bacterial development, and is thus regarded as a bacterial contamination reservoir [79].
Considering all the described advantages and disadvantages of both active and passive sampling methods and in order to assess microbial exposure, sampling approaches in occupational environments should comprise more than one type of sampling method [28,29,62,67,73,76]. Furthermore, and as it was seen in one study, settled dust should be included in sampling protocols combined with impaction methods because when these two methods are combined, the sensitivity of the assessment increases, and the impaction samplers' shortcomings are eliminated [58,80].
The majority of articles (15 out of 23) relied solely on culture-based methods to perform microorganisms' identification; nevertheless, and as expected, this assay also has its drawbacks that may influence the studies accuracy, such as the specificities of each species (growth rates and requirements), that can affect the other species in a mixed culture. A very common example regarding growth rate, is the overgrowth of some species that limit the growth of other species due to chemical competition [74].
Molecular tools are well known for their features of precision, high analytical sensitivity of detection, speed, and the ability to detect and identify dead or dormant microorganisms, as well as toxigenic strains from microorganisms [58,74,80,81,82]. However, culture-based methods should be used every time that the exposure route is mainly happening by inhalation, due to the reasons addressed before [56,57]. Thus, culture-based methods and molecular tools should be used side by side as it was seen in a few studies (4 out of 23) of this review.
Regarding the contamination present in all studies, as previously mentioned, majority of studies reported airborne fungi as a potential agent for occupational health effects (10 out of 23) since the prevalent genera were Penicillium (9 out 23) and Aspergillus (4 out of 23). Aspergillus sp. can be found everywhere and are easily disseminated in the air. Because the conidia of the Aspergillus genus are so small, they can readily be inhaled and colonize the upper and lower respiratory tracts of those who have been exposed [83,84]. Therefore, and as a consequence of a high exposure to opportunistic Aspergillus sp. (both in clinical and environment) the number of infections in immunocompromised patients has increased, as well as the antifungal resistance. It is known that Aspergillus species with a pathogenic potential, such as A. flavus, A. niger, A. terreus, A. versicolor, A. calidoustus, and A. nidulans [29,85], can lead to several health outcomes such as allergic bronchopulmonary aspergillosis and chronic pulmonary aspergillosis [58,86]. Additionally, it is also crucial to evaluate those species resistance to azoles, as it was performed in two studies of this review, in which the authors made a screening for A. fumigatus susceptibility to azoles. Azole resistance is a growing issue in A. fumigatus, threatening clinical improvements made possible by the use of azole antifungals in the treatment of Aspergillus-related disorders [28]. While some fungal species have innate azole resistance, acquired azole resistance has been found in fungi from occupational environments, such as sawmills, where azole fungicides (14-alpha demethylase inhibitors, DMI) used for timber preservation may exert some selection pressure on fungal populations [29]. Therefore, the use of azole fungicides to protect the wood reinforces the idea of performing a screening of susceptibility to azoles, specifically in this occupational environment.
Despite the methods used for the microbial occupational exposure assessment in these studies, it is important to highlight other methods and analysis that allowed a more complete assessment of sawmills' workers occupational exposure, such as the assessment to fungal allergens [87]. Sawmill workers are exposed to large levels of allergenic fungus on a regular basis, which can cause respiratory problems and asthma [8,87,88]. Microscopical spore counts and culture-based approaches have historically been used to measure fungus exposure [89]. There are, however, various immunoassays to measure environmental antigens [90] like the enzyme-linked immunosorbent test (ELISA) [87]. Another method commonly used in the studies of this review (9 out of 23) was the limulus amoebocyte lysate assay (LAL) to analyze and quantify endotoxins, and the field emission scanning electron microscopy (FESEM) to analyze fungal particles.
It is important to highlight that none of the studies included mycotoxins assessment. Mycotoxins are secondary metabolites created by fungi, and together with endotoxins and glucans, they make products of fungi and bacteria that are present in the organic dust produced by organic materials, including soil, plants, animals, food, and faeces, and inhaled by workers in a variety of industries [91]. Some mycotoxins can have serious human health effects when ingested, but their health effects following inhalation or dermal contact are insufficiently documented [91].
Specific fungal genera, primarily Aspergillus, Penicillium, Alternaria, Fusarium, and Claviceps, produce mycotoxins [91,92,93], such as aflatoxin B1 (produced mainly by Aspergillus flavus and Aspergillus parasiticus), ochratoxin (produced by both Aspergillus and Penicillium), trichothecenes, zearalenone, fumonisins B1 and B2, and some emerging mycotoxins like fusaproliferin, moniliformin, beauvericin and enniatins (produced mainly by Fusarium species), ergot alkaloids, (produced by Claviceps) and altenuene, alternariol, alternariol methyl ether, altertoxin, and tenuazonic acid (produced by Alternaria species) [91,93,94,95]. Two of them (Penicillium and Aspergillus) were found with the highest prevalence in this setting.
Mycotoxins can exist in the environment even when no visible fungi are present [91,96], since they can withstand adverse environmental factors such as high or low temperatures and can persist long after the death and disintegration of the fungal species responsible for their production. Even after being exposed to temperatures such as boiling or roasting operations, they are difficult to eradicate or inactivate from the source [91,97]. The majority of mycotoxins are non-volatile, nevertheless, they can be found in airborne dust [88,92,93], as well as in fungal spores and fragments [91,96,97]. As a result, dust, spores, and hyphae fragments in the air can carry mycotoxins to the lungs [91,96,97]. Moreover, in other cases, exposure in the workplace happens primarily by inhalation, notably through airborne dust [88,93,94,95,98,99,100,101,102]. Mucous membrane irritation, skin rash, nausea, immune system suppression, acute or chronic liver damage, acute or chronic central nervous system damage, endocrine changes, and cancer are all signs and effects of inhaling mycotoxins [91,97,103,104,105].
As previously reported by Viegas and colleagues [91], although the health effects of exposure to some mycotoxins through eating of contaminated food are well documented, few research has looked into the health implications of mycotoxins through inhalation or skin contact and absorption, which are probably the main routes of exposure in the sawmills industry. To understand the main determinants that may have an impact on exposure, it is particularly important to properly characterize occupational exposure through the identification of current mycotoxins, their levels, duration, and main routes of exposure associated with specific occupational environments. In addition, to allow comparisons between research standardized techniques (sampling and analysis) are required [91].
Finally, the geographical distribution of the studies included in this review is also something to consider since most of them (15 out of 23) were conducted in Europe. Thus, it is evident that there is a lack of investigation regarding microbial exposure in this occupational environment in the rest of the world. Moreover, looking more closely at the distribution of studies in Europe, the imbalance in the various areas is also perceptible since most studies are from Northern Europe (6 out of 15) and Central Europe (8 out of 15), leaving areas like Western Europe and Southern Europe with one study each, and Eastern Europe without studies regarding this subject.
Combining the findings of this review with the lack of information, it is possible to highlight the need to increase investigation regarding microbial occupational exposure in sawmills all over the world. This paper's findings should be considered, when preparing sampling campaigns and laboratory resources, to achieve an accurate microbial occupational exposure assessment in Portuguese sawmills.
This review allowed to identify the sampling methods and assays already employed to assess occupational exposure to microbial contamination in sawmills and to identify the knowledge gaps in what concerns this risk characterization.
Sawmill workers are exposed to several microbial contaminants in their workplace. Exposure to bacteria and fungi has been already reported, as well as bacteria metabolites (namely endotoxins). However, mycotoxins' assessment was not yet performed and, therefore, the risk from this exposure was not estimated.
No papers were found reporting the occupational microbiological exposure in sawmills located in Portugal. Therefore, microbial occupational exposure assessment in Portuguese sawmills is crucial to better characterize this risk, and to identify the measures to be taken into account in order to protect the workers.
The following are available online at https://www.mdpi.com/article/10.3390/atmos13020266/s1, Table S1. PRISMA Checklist.
Conceptualization, M.D., C.V. and S.V.; methodology, M.D., C.V.; formal analysis, B.G., R.C. and M.D.; investigation, M.D. and C.V.; resources, M.D., C.V. and S.V.; writing—original draft preparation, M.D., C.V., P.P. and S.V.; writing—review and editing, M.D., C.V. and S.V.; supervision, C.V.; project administration, M.D and C.V.; funding acquisition, M.D.. All authors have read and agreed to the published version of the manuscript.
This research was funded by national funds through the FCT—Fundação para a Ciência e Tecnologia, I.P., within the scope of the PhD Grant UI/BD/151431/2021.
H&TRC authors gratefully acknowledge the FCT/MCTES national support through the UIDB/05608/2020 and UIDP/05608/2020.
Sawmill Market Share 2021: Global Industry Size, Growth, Trend, Demand, Top Players, Opportunities and Forecast to 2026 with Leading Regions and Countries Data. Available online: https://www.marketwatch.com/press-release/sawmill-market-share-2021-global-industry-size-growth-trend-demand-top-players-opportunities-and-forecast-to-2026-with-leading-regions-and-countries-data-2021-12-09 (accessed on 18 October 2021).
Cox, C.S.; Wathes, C.M. Bioaerosols Handbook; CRC Press: Boca Raton, FL, USA; Available online: https://www.taylorfrancis.com/books/edit/10.1201/9781003070023/bioaerosols-handbook-christopher-cox-christopher-wathes (accessed on 15 December 2021).
Demers, P.A.; Teschke, K.; Kennedy, S.M. What to do about softwood? A review of respiratory effects and recommendations regarding exposure limits. Am. J. Ind. Med. 1997, 31, 385–398. [Google Scholar] [CrossRef]
Dennekamp, M.; Demers, P.; Bartlett, K.; Davies, H.; Teschke, K. Endotoxin exposure among softwood lumber mill workers in the canadian province of british columbia. Ann. Agric. Environ. Med. 1999, 6, 141–146. [Google Scholar]
Enarson, D.A.; Chan-Yeung, M. Characterization of health effects of wood dust exposures. Am. J. Ind. Med 1990, 17, 33–38. [Google Scholar] [CrossRef] [PubMed]
Halpin, D.M.G.; Graneek, B.J.; Lacey, J.; Nieuwenhuijsen, M.J.; Williamson, P.A.M.; Venables, K.M.; Newman Taylor, A.J. Respiratory symptoms, immunological responses and aeroallergen concentrations at a sawmill. Occup. Environ. Med. 1994, 51, 165–172. [Google Scholar] [CrossRef][Green Version]
Whitehead, L.W. Health effects of wood dust-relevance for an occupational standard. Am. Ind. Hyg. Assoc. J. 1982, 43, 674–678. [Google Scholar] [CrossRef] [PubMed]
Dutkiewicz, J.; Krysińska-Traczyk, E.; Prazmo, Z.; Skoŕska, C.; Sitkowska, J. Exposure to airborne microorganisms in polish sawmills. Ann. Agric. Environ. Med. 2001, 8, 71–80. [Google Scholar]
Burry, J.N. Contact dermatitis from radiata pine. Contact Dermat. 1976, 2, 262–263. [Google Scholar] [CrossRef]
Demers, P.A.; Kennedy, S.M.; Teschke, K.; Davies, H.; Bartlett, K. In Proceedings of the 12th International Symposium on Epidemiology in Occupational Health (ISEOH), Harare, Zimbabwe, 16-19 September 1997; Abstract 38.
De Zotti, R.; Gubian, F. Asthma and rhinitis in wooding workers. Allergy Asthma Proc. 1996, 17, 199–203. [Google Scholar] [CrossRef] [PubMed]
Goldsmith, D.F.; Shy, C.M. Respiratory health effects from occupational exposure to wood dusts. Scand. J. Work Environ. Health 1988, 14, 1–15. [Google Scholar] [CrossRef][Green Version]
Hedenstierna, G.; Alexandersson, R.; Wimander, K.; Rosén, G. Exposure to terpenes: Effects on pulmonary function. Int. Arch. Occup. Environ. Health 1983, 51, 191–198. [Google Scholar] [CrossRef]
Malmberg, P.O.; Rask-Andersen, A.; Larsson, K.A.; Stjernberg, N.; Sundblad, B.M.; Eriksson, K. Increased bronchial responsiveness in workers sawing scots pine. Am. J. Respir. Crit. Care Med. 1996, 153, 948–952. [Google Scholar] [CrossRef]
Mandryk, J.; Alwis, K.U.; Hocking, A.D. Work-related symptoms and dose-response relationships for personal exposures and pulmonary function among woodworkers. Am. J. Ind. Med. 1999, 35, 481–490. [Google Scholar] [CrossRef]
Mandryk, J.; Alwis, K.U.; Hocking, A.D. Effects of personal exposures on pulmonary function and work-related symptoms among sawmill workers. Ann. Occup. Hyg. 2000, 44, 281–289. [Google Scholar] [CrossRef]
Health Effects of Exposure to Wood Dust & Wood Dust References|NIOSH|CDC. Available online: https://www.cdc.gov/niosh/docs/wooddust/default.html (accessed on 10 December 2021).
Dutkiewicz, J.; Skórska, C.; Dutkiewicz, E.; Matuszyk, A.; Sitkowska, J.; Krysińska-Traczyk, E. Response of sawmill workers to work-related airborne allergens. Ann. Agric. Environ. Med. 2001, 8, 81–90. [Google Scholar]
Belin, L. Sawmill alveolitis in sweden. Int. Arch. Allergy Appl. Immunol. 1987, 82, 440–443. [Google Scholar] [CrossRef] [PubMed]
Eduard, W. Assessment of Mould Spore Exposure and Relations to Symptoms in Wood Trimmers|Wda. Available online: https://library.wur.nl/WebQuery/wda/abstract/577407 (accessed on 7 December 2021).
Eduard, W.; Sandven, P.; Levy, F. Serum IgG antibodies to mold spores in two norwegian sawmill populations: Relationship to respiratory and other work-related symptoms. Am. J. Ind. Med. 1993, 24, 207–222. [Google Scholar] [CrossRef]
Jäppinen, P.; Haahtela, T.; Liira, J. Chip pile workers and mould exposure. A preliminary clinical and hygienic survey. Allergy 1987, 42, 545–548. [Google Scholar] [CrossRef]
Kolmodin-Hedman, B.; Blomquist, G.; Löfgren, F. Chipped wood as a source of mould exposure. Eur. J. Respir. Dis. Suppl. 1987, 154, 44–51. [Google Scholar]
Minárik, L.; Mayer, M.; Votrubová, V.; Ürgeová, N.; Dutkiewicz, J. Allergic alveolitis. Wiad Lek 2020, 73, 1593–1599. (In Polish) [Google Scholar]
Rask-Andersen, A.; Land, C.J.; Enlund, K.; Lundin, A. inhalation fever and respiratory symptoms in the trimming department of swedish sawmills. Am. J. Ind. Med. 1994, 25, 65–67. [Google Scholar] [CrossRef] [PubMed]
Van Assendelft, A.H.; Raitio, M.; Turkia, V. Fuel chip-induced hypersensitivity pneumonitis caused by penicillium species. Chest 1985, 87, 394–396. [Google Scholar] [CrossRef] [PubMed]
Wimander, K.; Belin, L. Recognition of allergic alveolitis in the trimming department of a swedish sawmill. Eur. J. Respir. Dis. Suppl. 1980, 107, 163–167. [Google Scholar]
Jeanvoine, A.; Rocchi, S.; Reboux, G.; Crini, N.; Crini, G.; Millon, L. Azole-resistant Aspergillus fumigatus in sawmills of Eastern France. J. Appl. Microbiol. 2017, 123, 172–184. [Google Scholar] [CrossRef] [PubMed]
Viegas, C.; Almeida, B.; Aranha Caetano, L.; Afanou, A.; Straumfors, A.; Veríssimo, C.; Gonçalves, P.; Sabino, R. Algorithm to assess the presence of Aspergillus fumigatus resistant strains: The case of norwegian sawmills. Int. J. Environ. Health Res. 2020, 12, 23. [Google Scholar] [CrossRef]
Gisi, U. Assessment of selection and resistance risk for demethylation inhibitor fungicides in Aspergillus fumigatus in Agriculture and medicine: A critical review. Pest Manag. Sci. 2014, 70, 352–364. [Google Scholar] [CrossRef]
Snelders, E.; Camps, S.M.T.; Karawajczyk, A.; Schaftenaar, G.; Kema, G.H.J.; van der Lee, H.A.; Klaassen, C.H.; Melchers, W.J.G.; Verweij, P.E. Triazole fungicides can induce cross-resistance to medical triazoles in Aspergillus fumigatus. PLoS ONE 2012, 7, e31801. [Google Scholar] [CrossRef][Green Version]
Chowdhary, A.; Kathuria, S.; Xu, J.; Meis, J.F. Emergence of azole-resistant aspergillus fumigatus strains due to agricultural azole use creates an increasing threat to human health. PLoS Pathog. 2013, 9, e1003633. [Google Scholar] [CrossRef]
Deslandes, L. Advisory committee on paper and wood products—Portugal general economic situation. Advisory Committee on Paper and Wood Products 2008, 1–5. [Google Scholar]
Associação das Indústrias de Madeira e Mobiliário de Portugal (AIMMP). Available online: https://aimmp.pt/ (accessed on 8 November 2021).
Moher, D.; Liberati, A.; Tetzlaff, J.; Altman, D.G.; Group, T.P. Preferred reporting items for systematic reviews and meta-analyses: The prisma statement. PLoS Med. 2009, 6, e1000097. [Google Scholar] [CrossRef] [PubMed][Green Version]
Afanou, K.A.; Eduard, W.; Laier Johnsen, H.B.; Straumfors, A. Fungal fragments and fungal aerosol composition in sawmills. Saudi J. Biol. Sci. 2018, 62, 559–570. [Google Scholar] [CrossRef][Green Version]
Straumfors, A.; Olsen, R.; Daae, H.L.; Afanou, A.; McLean, D.; Corbin, M.; Mannetje, A.; Ulvestad, B.; Bakke, B.; Johnsen, H.L.; et al. Exposure to wood dust, microbial components, and terpenes in the norwegian sawmill industry. Ann. Work. Expo. Health 2018, 62, 674–688. [Google Scholar] [CrossRef]
Straumfors, A.; Corbin, M.; McLean, D.; Mannetje, A.; Olsen, R.; Afanou, A.; Daae, H.-L.; Skare, Ø.; Ulvestad, B.; Laier Johnsen, H.; et al. Exposure determinants of wood dust, microbial components, resin acids and terpenes in the saw- and planer mill industry. Ann. Work. Expo. Health 2020, 64, 282–296. [Google Scholar] [CrossRef][Green Version]
Straumfors, A.; Foss, O.A.H.; Fuss, J.; Mollerup, S.K.; Kauserud, H.; Mundra, S. The inhalable mycobiome of sawmill workers: Exposure characterization and diversity. Appl. Environ. Microbiol. 2019, 85, e01448-19. [Google Scholar] [CrossRef]
Prażmo, Z.; Dutkiewicz, J.; Cholewa, G. Gram-negative bacteria associated with timber as a potential respiratory hazard for woodworkers. Aerobiologia 2000, 16, 275–279. [Google Scholar] [CrossRef]
Rogoziński, T.; Szwajkowska-Michałek, L.; Dolny, S.; Andrzejak, R.; Perkowski, J. The evaluation of microfungal contamination of dust created during woodworking in furniture factories. Med. Pract. 2015, 65, 705–713. [Google Scholar] [CrossRef]
Górny, R.L.; Gołofit-Szymczak, M.; Cyprowski, M.; Stobnicka-Kupiec, A. Nasal lavage as analytical tool in assessment of exposure to particulate and microbial aerosols in wood pellet production facilities. Sci. Total Environ. 2019, 697, 134018. [Google Scholar] [CrossRef] [PubMed]
Oppliger, A.; Rusca, S.; Charrière, N.; Vu Duc, T.; Droz, P.-O. Assessment of bioaerosols and inhalable dust exposure in swiss sawmills. Ann. Occup. Hyg. 2005, 49, 385–391. [Google Scholar] [CrossRef] [PubMed][Green Version]
Rusca, S.; Charrière, N.; Droz, P.O.; Oppliger, A. Effects of bioaerosol exposure on work-related symptoms among swiss sawmill workers. Int. Arch. Occup. Environ. Health 2008, 81, 415–421. [Google Scholar] [CrossRef] [PubMed]
Ljubičić Ćalušić, A.; Varnai, V.M.; Cavlović, A.O.; Segvić Klarić, M.; Beljo, R.; Prester, L.; Macan, J. Respiratory health and breath condensate acidity in sawmill workers. Int. Arch. Occup. Environ. Health 2013, 86, 815–825. [Google Scholar] [CrossRef] [PubMed]
Klarić, M.Š.; Varnai, V.M.; Calušić, A.L.; Macan, J. Occupational exposure to airborne fungi in two croatian sawmills and atopy in exposed workers. Ann. Agric. Environ. Med. 2012, 19, 213–219. [Google Scholar] [PubMed]
Roponen, M.; Seuri, M.; Nevalainen, A.; Hirvonen, M.-R. Fungal spores as such do not cause nasal inflammation in mold exposure. Inhal. Toxicol. 2002, 14, 541–549. [Google Scholar] [CrossRef] [PubMed]
Gioffrè, A.; Marramao, A.; Iannò, A. Airborne microorganisms, endotoxin, and dust concentration in wood factories in Italy. Ann. Occup. Hyg. 2012, 56, 161–169. [Google Scholar] [CrossRef][Green Version]
Duchaine, C.; Mériaux, A.; Thorne, P.S.; Cormier, Y. Assessment of particulates and bioaerosols in eastern Canadian sawmills. AIHAJ 2000, 61, 727–732. [Google Scholar] [CrossRef]
Duchaine, C.; Mériaux, A. Airborne microfungi from eastern canadian sawmills. Can. J. Microbiol. 2000, 46, 612–617. [Google Scholar] [CrossRef]
Verma, D.K.; Demers, C.; Shaw, D.; Verma, P.; Kurtz, L.; Finkelstein, M.; des Tombe, K.; Welton, T. Occupational health and safety issues in ontario sawmills and veneer/plywood plants: A pilot study. J. Environ. Public Health 2010, 2010, 526487. [Google Scholar] [CrossRef] [PubMed][Green Version]
Cormier, Y.; Mérlaux, A.; Duchaine, C. Respiratory health impact of working in sawmills in Eastern Canada. Arch. Environ. Health 2000, 55, 424–430. [Google Scholar] [CrossRef]
Veillette, M.; Cormier, Y.; Israël-Assayaq, E.; Meriaux, A.; Duchaine, C. Hypersensitivity pneumonitis in a hardwood processing plant related to heavy mold exposure. J. Occup. Environ. Hyg. 2006, 3, 301–307. [Google Scholar] [CrossRef] [PubMed]
Park, H.; Park, H.; Lee, I. Microbial exposure assessment in sawmill, livestock feed industry, and metal working fluids handling industry. Saf. Health Work. 2010, 1, 183–191. [Google Scholar] [CrossRef][Green Version]
Neghab, M.; Jabari, Z.; Kargar Shouroki, F. Functional disorders of the lung and symptoms of respiratory disease associated with occupational inhalation exposure to wood dust in iran. Epidemiol. Health 2018, 40, e2018031. [Google Scholar] [CrossRef] [PubMed]
Croston, T.L.; Nayak, A.P.; Lemons, A.R.; Goldsmith, W.T.; Gu, J.K.; Germolec, D.R.; Beezhold, D.H.; Green, B.J. Influence of Aspergillus fumigatus conidia viability on murine pulmonary MicroRNA and MRNA expression following subchronic inhalation exposure. Clin. Exp. Allergy 2016, 46, 1315–1327. [Google Scholar] [CrossRef][Green Version]
Dias, M.; Viegas, C. Fungal prevalence on waste industry—Literature review. In Encyclopedia of Mycology; Zaragoza, Ó., Casadevall, A., Eds.; Elsevier: Oxford, UK, 2021; pp. 99–106. [Google Scholar] [CrossRef]
Timm, M.; Madsen, A.M.; Hansen, J.V.; Moesby, L.; Hansen, E.W. Assessment of the total inflammatory potential of bioaerosols by using a granulocyte assay. Appl. Environ. Microbiol. 2009, 75, 7655–7662. [Google Scholar] [CrossRef][Green Version]
Beard, J.T.; Iachetta, F.A.; Lilleleht, L.U. APTI (Air Pollution Training Institute) Course 427: Combustion Evaluation, Student Manual; PB-80-207798; Associated Environmental Consultants: Charlottesville, VA, USA, 1980. [Google Scholar]
Santos, J.; Ramos, C.; Vaz-Velho, M.; Vasconcelos Pinto, M. Occupational exposure to biological agents. In Advances in Safety Management and Human Performance; Arezes, P.M., Boring, R.L., Eds.; Springer International Publishing: Cham, Switzerland, 2020; pp. 61–67. [Google Scholar]
Dias, M.; Sousa, P.; Viegas, C. Occupational exposure to bioburden in portuguese ambulances. In Occupational and Environmental Safety and Health III; Arezes, P.M., Baptista, J.S., Carneiro, P., Castelo Branco, J., Costa, N., Duarte, J., Guedes, J.C., et al., Eds.; Studies in Systems, Decision and Control; Springer International Publishing: Cham, Switzerland, 2022; pp. 167–173. [Google Scholar] [CrossRef]
Mao, J.; Tang, Y.; Wang, Y.; Huang, J.; Dong, X.; Chen, Z.; Lai, Y. Particulate matter capturing via naturally dried ZIF-8/Graphene aerogels under harsh conditions. iScience 2019, 16, 133–144. [Google Scholar] [CrossRef] [PubMed][Green Version]
International Labour Organization. Encyclopaedia of Occupational Health and Safety; International Labour Organization: Geneve, Switzerland, 1998. [Google Scholar]
Černá, K.; Wittlingerová, Z.; Zimová, M.; Janovský, Z. Methods of sampling airborne fungi in working environments of waste treatment facilities. Int. J. Occup. Med. Environ. Health 2016, 29, 493–502. [Google Scholar] [CrossRef] [PubMed]
Viegas, C.; Almeida, B.; Monteiro, A.; Paciência, I.; Rufo, J.C.; Carolino, E.; Quintal-Gomes, A.; Twarużek, M.; Kosicki, R.; Marchand, G.; et al. Settled dust assessment in clinical environment: Useful for the evaluation of a wider bioburden spectrum. Int. J. Environ. Health Res. 2019, 31, 160–178. [Google Scholar] [CrossRef] [PubMed]
Leppänen, H.K.; Täubel, M.; Jayaprakash, B.; Vepsäläinen, A.; Pasanen, P.; Hyvärinen, A. Quantitative assessment of microbes from samples of indoor air and dust. J. Expo. Sci. Environ. Epidemiol. 2018, 28, 231–241. [Google Scholar] [CrossRef]
Meadow, J.F.; Altrichter, A.E.; Kembel, S.W.; Kline, J.; Mhuireach, G.; Moriyama, M.; Northcutt, D.; O'Connor, T.K.; Womack, A.M.; Brown, G.Z.; et al. Indoor airborne bacterial communities are influenced by ventilation, occupancy, and outdoor air source. Indoor Air 2014, 24, 41–48. [Google Scholar] [CrossRef]
Emerson, J.B.; Keady, P.B.; Brewer, T.E.; Clements, N.; Morgan, E.E.; Awerbuch, J.; Miller, S.L.; Fierer, N. Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 colorado front range flood. Environ. Sci. Technol. 2015, 49, 2675–2684. [Google Scholar] [CrossRef]
Barberán, A.; Dunn, R.R.; Reich, B.J.; Pacifici, K.; Laber, E.B.; Menninger, H.L.; Morton, J.M.; Henley, J.B.; Leff, J.W.; Miller, S.L.; et al. The ecology of microscopic life in household dust. Proc. Biol. Sci. 2015, 282, 1139. [Google Scholar] [CrossRef]
Saejiw, N.; Chaiear, N.; Sadhra, S. Exposure to wood dust and its particle size distribution in a rubberwood sawmill in Thailand. J. Occup. Environ. Hyg. 2009, 6, 483–490. [Google Scholar] [CrossRef]
Hinds, W.C. Basic for size-selective sampling for wood dust. Appl. Ind. Hyg. 1988, 3, 67–72. [Google Scholar] [CrossRef]
Environmental Sciences—Editorial Contacts|Springer. Available online: https://www.springer.com/gp/environmental-sciences/contact-us?gclid=Cj0KCQiAweaNBhDEARIsAJ5hwbfngBdU3g9WbmW82SZiL1UWlTNh5X98xQXKlgPqR1IA2ymwa6znC6gaAo2FEALw_wcB (accessed on 5 November 2021).
Viegas, C.; Almeida, B.; Dias, M.; Caetano, L.A.; Carolino, E.; Gomes, A.Q.; Faria, T.; Martins, V.; Marta Almeida, S. Assessment of Children's Potential Exposure to Bioburden in Indoor Environments. Atmosphere 2020, 11, 993. [Google Scholar] [CrossRef]
Viegas, C. Sampling Methods for an Accurate Mycobiota Occupational Exposure Assessment: Overview of Several Ongoing Projects; Taylor & Francis: Abingdon, UK, 2018; pp. 7–11. [Google Scholar] [CrossRef]
Park, J.-H.; Sulyok, M.; Lemons, A.R.; Green, B.J.; Cox-Ganser, J.M. Characterization of fungi in office dust: Comparing results of microbial secondary metabolites, fungal internal transcribed spacer region sequencing, viable culture and other microbial indices. Indoor Air 2018, 28, 708–720. [Google Scholar] [CrossRef] [PubMed]
Viegas, C.; Faria, T.; Caetano, L.A.; Carolino, E.; Quintal-Gomes, A.; Twarużek, M.; Kosicki, R.; Viegas, S. Characterization of occupational exposure to fungal burden in portuguese bakeries. Microorganisms 2019, 7, 234. [Google Scholar] [CrossRef] [PubMed][Green Version]
Viegas, C.; Gomes, B.; Dias, M.; Carolino, E.; Aranha Caetano, L. Aspergillus section Fumigati in firefighter headquarters. Microorganisms 2021, 9, 2112. [Google Scholar] [CrossRef]
Viegas, C.; Almeida, B.; Monteiro, A.; Paciência, I.; Rufo, J.; Viegas, S. EXPOsE: Establishing protocols to assess occupational exposure to bioburden in clinical environments. In Proceedings of the OH2019—The Premier Conference for Occupational Hygiene in the UK, Brighton, UK, 1–4 April 2019. [Google Scholar]
Bouillard, L.; Michel, O.; Dramaix, M.; Devleeschouwer, M. Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings. Ann. Agric. Environ. Med. 2005, 12, 187–192. [Google Scholar] [PubMed]
Viegas, C.; Faria, T.; Meneses, M.; Carolino, E.; Viegas, S.; Gomes, A.Q.; Sabino, R. Analysis of surfaces for characterization of fungal burden—Does it matter? Int. J. Occup. Med. Environ. Health 2016, 29, 623–632. [Google Scholar] [CrossRef][Green Version]
Amann, R.I.; Ludwig, W.; Schleifer, K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 1995, 59, 143–169. [Google Scholar] [CrossRef]
MacNeil, L.; Kauri, T.; Robertson, W. Molecular techniques and their potential application in monitoring the microbiological quality of indoor air. Can. J. Microbiol. 1995, 41, 657–665. [Google Scholar] [CrossRef]
Walsh, T.J.; Anaissie, E.J.; Denning, D.W.; Herbrecht, R.; Kontoyiannis, D.P.; Marr, K.A.; Morrison, V.A.; Segal, B.H.; Steinbach, W.J.; Stevens, D.A.; et al. Infectious diseases society of america. treatment of aspergillosis: Clinical practice guidelines of the infectious diseases society of America. Clin. Infect. Dis. 2008, 46, 327–360. [Google Scholar] [CrossRef]
Viegas, C.; Caetano, L.A.; Viegas, S. Occupational exposure to Aspergillus Section Fumigati: Tackling the knowledge gap in Portugal. Environ. Res. 2021, 194, 110674. [Google Scholar] [CrossRef]
Varga, V.; Kocsubé, S.; Szigeti, G.; Baranyi, N.; Téth, B. Aspergillus mycotoxins. In Molecular Biology of Food and Water Borne Mycotoxigenic and Mycotic Fungi; Paterson, R.R.M., Lima, N., Eds.; CRC Press: Boca Raton, FL, USA, 2015; pp. 165–186. [Google Scholar]
Lamoth, F. Aspergillus fumigatus-related species in clinical practice. Front. Microbiol. 2016, 7, 683. [Google Scholar] [CrossRef] [PubMed][Green Version]
Prester, L.; Macan, J. Determination of Alt a 1 (Alternaria alternata) in poultry farms and a sawmill using ELISA. Med. Mycol. 2010, 48, 298–302. [Google Scholar] [CrossRef] [PubMed][Green Version]
Hessel, P.A.; Herbert, F.A.; Melenka, L.S.; Yoshida, K.; Michaelchuk, D.; Nakaza, M. Lung health in sawmill workers exposed to pine and spruce. Chest 1995, 108, 642–646. [Google Scholar] [CrossRef]
Niemeier, R.T.; Sivasubramani, S.K.; Reponen, T.; Grinshpun, S.A. Assessment of fungal contamination in moldy homes: Comparison of different methods. J. Occup. Environ. Hyg. 2006, 3, 262–273. [Google Scholar] [CrossRef] [PubMed][Green Version]
Barnes, C.; Portnoy, J.; Sever, M.; Arbes, S.; Vaughn, B.; Zeldin, D.C. Comparison of enzyme immunoassay-based assays for environmental Alternaria alternata. Ann. Allergy Asthma Immunol. 2006, 97, 350–356. [Google Scholar] [CrossRef]
Iversen, M.; Kirychuk, S.; Drost, H.; Jacobson, L. Human health effects of dust exposure in animal confinement buildings. J. Agric. Saf. Health 2000, 6, 283–288. [Google Scholar] [CrossRef]
Viegas, S.; Viegas, C.; Oppliger, A. Occupational exposure to mycotoxins: Current knowledge and prospects. Ann. Work. Expo. Health 2018, 62, 923–941. [Google Scholar] [CrossRef]
Bennett, J.W.; Klich, M. Mycotoxins. Clin. Microbiol Rev. 2003, 16, 497–516. [Google Scholar] [CrossRef][Green Version]
Marin, S.; Ramos, A.J.; Cano-Sancho, G.; Sanchis, V. Mycotoxins: Occurrence, toxicology, and exposure assessment. Food Chem. Toxicol. 2013, 60, 218–237. [Google Scholar] [CrossRef]
Barkai-Golan, R.; Paster, N. Mouldy fruits and vegetables as a source of mycotoxins: Part 1. World Mycotoxin J. 2008, 1, 147–159. [Google Scholar] [CrossRef]
Huttunen, K.; Korkalainen, M. Microbial secondary metabolites and knowledge on inhalation effects. In Exposure to Microbiological Agents in Indoor and Occupational Environments; Viegas, C., Viegas, S., Quintal Gomes, A., Taubel, M., Sabino, R., Eds.; Springer Nature: Cham, Switzerland, 2017. [Google Scholar] [CrossRef]
Lavicoli, I.; Brera, C.; Carelli, G.; Caputi, R.; Marinaccio, A.; Miraglia, M. External and internal dose in subjects occupationally exposed to ochratoxin A. Int. Arch. Occup. Environ. Health 2002, 75, 381–386. [Google Scholar] [CrossRef][Green Version]
Halstensen, A.S. Species-specific fungal DNA in airborne dust as surrogate for occupational mycotoxin exposure? Int. J. Mol. Sci. 2008, 9, 2543–2558. [Google Scholar] [CrossRef] [PubMed]
Peraica, M.; Radić, B.; Lucić, A.; Pavlović, M. Toxic effects of mycotoxins in humans. Bull. World Health Organ. 1999, 77, 754–766. [Google Scholar] [PubMed]
Flannigan, B. Mycotoxins in the air. Int. Biodeterior. 1987, 23, 73–78. [Google Scholar] [CrossRef]
Brera, C.; Caputi, R.; Miraglia, M.; Iavicoli, I.; Salerno, A.; Carelli, G. Exposure assessment to mycotoxins in workplaces: Aflatoxins and ochratoxin A occurrence in airborne dusts and human sera. Microchem. J. 2002, 73, 167–173. [Google Scholar] [CrossRef]
Brasel, T.L.; Martin, J.M.; Carriker, C.G.; Wilson, S.C.; Straus, D.C. Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins in the indoor environment. Appl. Environ. Microbiol. 2005, 71, 7376–7388. [Google Scholar] [CrossRef][Green Version]
Mayer, S.; Curtui, V.; Usleber, E.; Gareis, M. Airborne mycotoxins in dust from grain elevators. Mycotoxin Res. 2007, 23, 94–100. [Google Scholar] [CrossRef]
Mayer, S. Occupational exposure to mycotoxins and preventive measures. In Environmental Mycology in Public Health: Fungi and Mycotoxins Risk Assessment and Mana-Gement; Viegas, C., Pinheiro, A.C., Sabino, R., Viegas, S., Brandão, J., Verissimo, C., Eds.; Academic Press: Waltham, MA, USA, 2007; ISBN 978-0-12-411471-5. [Google Scholar]
Olsen, J.H.; Dragsted, L.; Autrup, H. Cancer risk and occupational exposure to aflatoxins in denmark. Br. J. Cancer 1988, 58, 392–396. [Google Scholar] [CrossRef] [PubMed][Green Version]
Figure 1. PRISMA based selection of articles.
Table 1. Inclusion and exclusion criteria in the articles selected.
Exclusion Criteria
Articles published in the English language; Articles published in other languages
Articles published from 1 January 2000
to 30 September 2021 Articles published prior to 2000
Articles reporting findings from any country
Articles related to microbial exposure assessment in sawmills Articles related exclusively to biologic samples from workers or without mention microbial exposure.
Original scientific articles on the topic Abstracts of congress, reports, reviews/state of the art articles
Table 2. Data selected from the chosen papers.
Occupational Environments
Main Findings Concerning Microbiological Contamination
Assessment of Particulate and Bioaerosol in Eastern Canadian Sawmills Sawmills
(N = 17) Active—Filtration, Impaction and
Impinger Morphol. id. (Fungi) Penicillium sp. was the predominant genera, with up to 40 different species identified. The highest levels of molds, bacteria were associated to debarking site. Planing sites were the most highly dust contaminated. Airborne biological contaminants vary between working sites and their microflora diverge from that previously described in European sawmills. [49]
Airborne microfungi from eastern Canadian sawmills Sawmills
(N = 17) Active—Impaction and
Impinger Morphol. id. (Fungi) In eastern Canadian sawmills, the micoflora is dominated by Penicillium species. Fungi identified in European sawmills were not frequently identified in eastern Canadian sawmills. [50]
Assessment of Bioaerosols and Inhalable Dust Exposure in Swiss Sawmills Small (N = 8)
and medium size Sawmills (N = 2) Active—Filtration,
Impaction and Impinger Morphol. id. (Fungi) All sawmills exceeded the Swiss occupational exposure guideline of 1000 Colony Forming Units CFU. m3. Two sawmills for total bacteria and one sawmill for Gram-negative bacteria did not comply with Swiss occupational exposure guideline. Gram-positive bacteria, mainly Bacillus spp. were prevalent among the plates. The most frequent fungal genera was Penicillium sp. [43]
Effects of biaerosol exposure on work-related symptoms among Swiss sawmills workers Sawmills (N = 12) Active—Impaction Morphol. id. (Fungi) The composition of airborne fungi exceeded the limit recommended by the Swiss National Insurance. Fungal level influenced the occurrence of bronchial syndrome. Airborne fungi in the sawmill environment are potential agents for occupational health effects. [44]
Microbial Exposure Assessment in Sawmill, Livestock Feed Industry, and Metal Working Fluids Handling Industry Livestock feed Industry (N = 3), Metal working Fluids Hadling Industry (N = 2)
and Sawmills (N = 5) Active—Impaction Morphol. id. (Fungi) Airborne concentrarion of bacteria and fungi were 1.864 and 2.252 CFU·m3.
The ratio I/O was 3.7 and 4.1 for bacteria and fungi respectively. The respiratory fraction of bacteria was 57.7%, and fungi was 83.7%. Bioaerosol density was the highest in sawmills. [54]
Occupational Health and Safety Issues in Ontario Sawmills and Veneer/Plywood Plants: A Pilot Study Sawmill (N = 8)
and venner/
plywood manufacturing industry (N = 12) Active—Impaction Morphol. id. (Fungi) Fungal bioaerosols vary between different indoor locations. [51]
Respiratory Health and breath condensate acidity in sawmill workers Sawmills (N = 2) Active—Impaction and filtration Morphol. id. (Fungi) Airborne dust concentrations were below the threshold limit value. Airborne moulds were at levels able to induce inflammatory response in the airways. Significant differences between sawmills were observed regarding mould levels. [45]
Occupational exposure to airborne fungi in two Croatian sawmills and atopy in exposed workers Sawmill (N = 2) Active—Impaction Morphol. id. (Fungi) Airborne fungi present health hazardous levels (above 104 m−3) in one sawmill. Fungal levels were related to saw working sites. The prevalent fungal genera were Penicillium (50–100%), Paecilomyces (43–100%) and Chrysonilia (33–100%). Other airborne fungi that were recurrent, but with lower frequency were: A. niger (15–71%), Trichoderma sp. (8–40%), Rhizopus sp. (8–20%) and A. flavus (2–15%). [46]
Fungal fragments and fungal aerossol composition in Sawmills Sawmills (N = 2) Active—Filtration GM (Fungal fragments); FESEM (Fungi) The composition of fungal aerosols comprised in average: submicronic fragments (9%), large fragments (62%) and spores (29%). The ratio of spores was higher in saw departments. Fungal fragments were most prevalent in sorting and green timber departments. The season influenced significatively the fungal aerosol density but not the composition. Fungal fragments should be included in exposure-response studies. [36]
Exposure to Wood dust, Microbial Components, and Terpenes in the Norwegian Sawmill Industry Sawmills (N = 11) Active—Filtration GM (Fungal fragments
FESEM (Fungi) The GM of both thoracic and inhalabe expoure was higher in various departments. The mean fungal spore was 0.41 × 105 spores·m−3. Exposure to spores was high in dry timber departments. High levels of thoracic fungal spores was also found in workers associated to sorting of dry timber. Microbial exposure had the highest levels in workers working with green timber. [37]
Algorithm to assess the presence of Aspergillus
fumigatus resistant strains: The case of Norwegian
sawmills Industrial sawmills (N = 11) Active—Filtration Morphol. id.;
Screening—EUCAST method; Mol. tools—DNA sequencing (Fungi) Fungal contamination ranged from 0–2.7 × 105 CFU·m−3 in malt extract agar (MEA) and from 0–1.3 × 105 CFU·m−3 in dichloran-glycerol agar (DG18). The prevalent species were Chrysonilia sitophila (65.20%), Mucor sp. (23.86%) and Rhizopus sp. (10.75%) on MEA. On DG18, Penicillium sp. (0.26%) and Aspergillus sp. (0.14%) were frequent. In MEA, section Fumigati was found. Whereas in DG18, four different Aspergillus sections were detected: Circumdati; Candidi; Fumigati; Nigri. Two Fumigati isolates were able to grow in the presence of one or two medical triazoles. One isolate was found to be a TR34/L98H mutant. Fungicides used at sawmills may decrease fungal sensibility to azole drug [29]
Respiratory Healh Impact of Working in Sawmills in Eastern Canada Sawmills (N = 17) Active—Impaction and Impinger Morphol. id. (Fungi) The most frequently fungal identified were Penicillium myczinskii, P. spinulosum, P.fellutanum, Trichoderma sp. and Paecilomyces sp. Working in a Québec sawmill does not constitute a clinically revelant respiratory Health risk. [52]
Gram-negative bacteria associated with timber as a potential respiratory hazard for woodworkers Sawmills (N = 1) Active—Impaction
Passive—Wood samples Biochem. tests (Bacteria) Enterobacteriaceae strains, by majority Enterobacter sp. and Rahnella sp. comprised 70–75% of Gram-negative bacteria isolates from pine and beech wood and sawmill air samples. During processing of beech wood high levels of Gram-negative bacteria were released into air, when comparing with pine wood processing. The aerial exposure to Gram-negative bacteria possessing endotoxic and allergenic properties poses a potential risk to workers health. [40]
Functional disorders of the lung and symptoms of respiratory disease associated with occupational inhalation exposure to wood dust in Iran Sawmills (N = 20) Active—Impaction and filtration Morphol. id. (Fungi); Biochem. tests (Bacteria)
The prevalent Gram-negative bacteria were Pseudomonadaceae, Klebsiella pneumoniae and Rhinoscleromatis sp. Penicillium sp. and Fusarium sp. were the predominant fungi. Respiratory symptoms were significantly more frequent among exposed workers. [55]
Exposure Determinants of Wood Dust, Microbial Components, Resin Acids and Terpenes in the Saw- and Planer Mill Industry Sawmills (N = 11) Active -Filtration FESEM (Fungi) The highest microbial exposure were estimated in the green part of the sawmills. Exposure to fungal spores were relatively low and similar among most departments. Season and wood type had a large effect on the estimated exposure. [38]
The Inhalable Mycobiome of Sawmill Workers: Exposure Characterization and Diversity Industrial sawmill, sorting mill and planer mill companies processing spruce or pine (N = 11) Active -Filtration FESEM (Fungi);
GM (Fungal fragments);
Mol. tools (DNA-sequencing) (Fungi) Ascomycota was the common phylum detected (50.3%) followed by Basidiomycota (45.6%). Operational taxonomic units were higher during spure processing when compared to pine processing. The highest fungal diversity was obtained in saw department. The fungal compositions of the exposures differs between seasons, sawmills, wood types and departments. A risk assessment based on the fungal diversity diferences should be performed. [39]
Exposure to airborne microorganisms in polish sawmills Sawmills (N = 4) Active—Impaction GM (Fungal fragments) Morphol. id. (Fungi);
Biochem. tests (Bacteria) Microorganisms load was higher in sawmills processing coniferous wood when compared to those processing deciduous wood. Allergenic fungi (the majority Aspergillus fumigatus) were predominant in air samples when debarking. During first-cut frame airborne microflora as mostly constituted by endotoxin producing Gram-negative bacteria belonging to Rahnella genus developing in the sapwood of pine. Regarding bacteria diversity, 34 species or genera were identified. Also, 21 species or genera of fungi were found in the air of sawmills. Workers of Polish sawmills may be exposed during some tasks to airborne microorganisms posing respiratory hazard. [8]
Fungal Spores As Such Do Not Cause
Nasal Inflammation In Mold Exposure Sawmill (N = 11) Active—Impinger (personal samplers) Epifluorescence technique CAMNEA method (Fungal spores) Rhizopus and Penicillium were the predominant genera. Proinflammatory potential of microbial exposure seems to be related to the type of microbial bioaerosols in the occupational environment. [47]
Airborne Microorganisms, Endotoxin and Dust Concentration in Wood Factories in Italy 6 Sawmills and carpentries (N = 6) Active—Impaction and filtration Morphol. id. (Fungi);
Biochem. tests (Bacteria)
In air samples from wood factories 19 species of Gram-negative and 14 species of Gram-positive bacteria were identified. Whereas, 18 species of mould were found, some having allergenic, immunotoxic properties. Gram-negative bacteria levels were higher in these workplaces. Penicillium sp. and Alternaria alternata were identified in low densities. Workers in wood factories may be exposed to high levels of inhalable dust. [48]
The evaluation of microfungal contamination of dust
Created during woodworking in furniture factories Furniture factories (N = 3) Passive—settled dust Morphol. id. (Fungi)
The most frequent fungi in the tested dust were Penicillium sp. and Aspergillus sp. Trichoderma genus has been isolated.Airborne fungal may be associated with the wood dust, posing a health hazard for exposed workers. [41]
Hypersensitivity Pneumonitis in a Hardwood Processing
Plant Related to Heavy Mold Exposure Hardwood processing plant (N = 1) Active—Impaction
Passive—Dust and surface samples from wood planks Morphol. id. (Fungi)
Paecilomyces sp. growth was observed on the surface of the dried processed wood in the index plant. Penicillium sp. was prevalent on green wood. Wood quality (moisture content, time of storage prior to drying) and processes may influence wood contamination workers exposure. [53]
Nasal lavage and analytical tool in Assessment of exposure to particulate and microbial aerossol in wood pellet production facilities 10 Pellet production facilities (N = 10) Active—Impaction and filtration Morphol. id (Fungi and Bacteria);
Mol. tools (DNA sequencing) (Fungi and Bacteria)
Biochem. tests (Bacteria) Among isolated, bacterial pathogens from Streptomyces genus and Aspergillus fumigatus pathogenic fungus were identified. Concerning microorganisms size distribution, the highest bacteria load can reach the nasal and oral cavities as well as secondary bronchi. In case of fungi, the highest load can reach the nasal and oral cavities. Microbiota diversity in the indoor was higher when compared to the outdoor, suggesting that the processed material act as an active emission source. [42]
Azole-resistant Aspergillus fumigatus in sawmills in Eastern France Sawmills (N = 20) Active—Impaction
Passive—Settled dust Morphol. id.
Screening (EUCAST and E-test);
Mol. tools (DNA- Seq) (Fungi) Azole resistante A. fumigatus was collected in 20 samples from a total of 600 settled dust samples. From the A.fumigatus obtained strains, 83% had TR34/L98H mutation. A greater number of resistant strains was collected in sawmills that applied fungicide products. Azole-resistant mutations seems to be associated to the azole fungicide formulation and quantities of azole. [28]
Morphol. id.—Morphological identification; Mol. tools—Molecular tools; Biochem. tests—Biochemical tests; GM—Gravimetric Measurement; FESEM—Immunolabeling method for field emission scanning electron microscope.
Dias, M.; Gomes, B.; Cervantes, R.; Pena, P.; Viegas, S.; Viegas, C. Microbial Occupational Exposure Assessments in Sawmills—A Review. Atmosphere 2022, 13, 266. https://doi.org/10.3390/atmos13020266
Dias M, Gomes B, Cervantes R, Pena P, Viegas S, Viegas C. Microbial Occupational Exposure Assessments in Sawmills—A Review. Atmosphere. 2022; 13(2):266. https://doi.org/10.3390/atmos13020266
Dias, Marta, Bianca Gomes, Renata Cervantes, Pedro Pena, Susana Viegas, and Carla Viegas. 2022. "Microbial Occupational Exposure Assessments in Sawmills—A Review" Atmosphere 13, no. 2: 266. https://doi.org/10.3390/atmos13020266
Atmosphere, EISSN 2073-4433, Published by MDPI


Legislature includes $200M for Port of Alaska rebuild in budget
The Alaska Legislature has included $200 million in funding in the capital budget to help rebuild the Port of Alaska.
That's a third of the hefty $600 million state funding request made by Anchorage Mayor Dave Bronson and the Assembly in the city's 2022 funding priorities, which Bronson and some Assembly members had lobbied for in Juneau.
The $200 million would go toward the $1.1 billion cost of replacing the port's cargo docks. The entire Port of Alaska modernization project, which will take about 10 years, is estimated to cost a total of $1.85 billion, according to city officials.
The port's infrastructure is failing, threatening a critical piece of the state's supply chain.
Political resistance to spending state money on what many have viewed as an Anchorage-specific problem has stymied previous city requests for port funding. But Alaska is expecting billions in new revenue after a wartime surge in oil prices, opening the door for broader support.
Anchorage should receive half of the $200 million in state funds over the next two fiscal years, but the other $100 million comes with a contingency: The state will provide the money only if the city gets a matching $100 million in federal funds, said Ross Risvold, senior finance officer for Anchorage's Finance Department. The city is currently reviewing and applying for federal funding opportunities, he said.
"We're leaving, as the saying goes, no stone unturned," Risvold said.
While the state funds would cover a portion of the project's price tag, the infusion would keep it moving ahead, while total costs will be spread out over the project's lifetime, said Jim Jager, director of external affairs for the port.
"The state money will also be key to unlocking federal money," he said. Federal grants require a percentage match in non-federal funds.
Alaska Gov. Mike Dunleavy could still veto funding for the port. City officials say they expect the governor will keep the funding in the budget. The governor has about two weeks left to make decisions on the budget before he signs it.
Shannon Mason, deputy press secretary for the governor's office, declined to say whether Dunleavy will strike the port funding.
"We are reviewing the budget and ensuring that the use of public funds is in the interest of all Alaskans," Mason said by email.
It's also not yet clear how much money the city will receive from a legal settlement related to a failed effort to expand the port. The U.S. Department of Transportation's Maritime Administration, which was found at fault, has appealed a December court ruling, and a decision on the appeal could take a year or more, city officials say.
The city also plans to borrow money to pay for the project through low interest rate loans via the federal government and through bonds, Risvold said.
But much of the cost of borrowing will be repaid through raising fees on deliveries to the port. That cost is expected to fall back on Alaskans purchasing goods that come through the port, according to the city. State and federal grants are intended to help to defray the tariffs and the resulting increase in prices.
State Rep. Andy Josephson, D-Anchorage, said the usually politically divided Anchorage delegation worked in a rare bipartisan effort to get port funding into the budget.
"It was sort of inarguable that everywhere other than Southeast benefits from the Port of Alaska," Josephson said. "… The tariffs that are going to be charged are going to be very, very aggressive and will impact everybody, so it's 'pay me now or pay me later.' "
Kolby Hickel, deputy municipal manager, said that 90% of Alaskans rely on goods shipped through the port, including fuel, food, construction supplies, ammunition, parts for vehicles and tools.
The 2018 earthquake caused significant damage to the port, and the city says it is at "imminent risk of failure" if an earthquake of a similar size happens.
Grocery stores only have about six days of food on hand, and a failure of the port would be a crisis, Hickel said.
The new petroleum and cement terminal will be finished in June, the first of five phases in rebuilding and repairing the port, which the city aims to complete in 2035.
Construction of a new port administration building is slated to begin this summer, said Jager.
Work to stabilize and remove a northern part of the embankment is scheduled for the following two summers, he said.
The earthwork and a defective wall north of the docks were made as part of the failed port expansion project, and it must all be removed before new cargo docks can be built, Jager said.
"It looks like land, smells like land, tastes like land. It's not really land. It's not stable. It's behind an unstable wall," Jager said. "And the danger is that either due to the piling that they installed incorrectly corroding, or use due to seismic action, or other things, that wall is going to fail, land will slide into the navigation channel, and even potentially take out the existing dock."
Once the first phase of stabilization is complete, construction of two new cargo terminals can begin.
The city is currently working on the permitting, design and build of the terminal replacements, Hickel said.


What Are the Causes of Thickness in Breast Tissue?
By: M. Gideon Hoyle
Breast thickening causes
How to identify duck eggs
Menopause & bumps on the labia
What Is Normal Glucose Blood Test?
Diosmin and hesperidin pregnancy safety
Thickened breast tissue is an abnormal density detectable during self-examination or a doctor's exam. Causes for this condition range from common benign changes in the breast to the presence of breast cancer.
Fibrocystic
Fibrocystic breast changes involve the formation of dense, fibrous tissue or fluid-filled cysts inside the breast, according to the American Cancer Society (ACS). Roughly half of all women experience them at some time. While they typically present little danger, some cases may indicate an increased risk for breast cancer.
Fibroadenomas
Benign tumours called fibroadenomas may create areas of breast tissue that feel like marbles, according to the ACS. Depending on a tumour's rate of growth, doctors may recommend surgically removing it.
Phyllodes Tumors
Phyllodes tumours form from glandular and connective tissue in the breast, and typically manifest as a painless lump. While most cases are benign, a small number indicate the presence of cancer, according to the ACS.
The Merck Manual of Health and Aging notes that thickening of the breast may mark the onset of breast cancer. In some cases, the breast tissue remains normal while hard lumps appear in nearby lymph nodes.
The Mayo Clinic notes that the presence of fibrocystic changes may increase the difficulty of breast self-examinations.
American Cancer Society: Non-Cancerous Breast Conditions
The Mayo Clinic: Fibrocystic Breasts
M. Gideon Hoyle is a writer living outside of Houston. Previously, he produced brochures and a wide variety of other materials for a nonprofit educational foundation. He now specializes in topics related to health, exercise and nutrition, publishing for various websites.


Amanda Loves Words
June...
When those of us in Texas start hiding indoors.
Amanda Waters Jun 13, 2020 2
Welcome to another month of intense shared emotional experiences! I, for one, have alternated between sadness, anger, disappointment, hope, and a touch of determination. I've been reminded lately of the importance of taking it one day at a time, keeping my eyes on Jesus, listening to others, and doing my best. And in the meantime...we keep reading…
A Journal of the Plague Year, by Daniel Defoe came across my reading list as a review book (even though it's not new by any stretch of the imagination), and it turned out to be a lot more fascinating than I expected. It's an adaptation of a journal written about the Bubonic Plague in London in 1665 (incidentally, the year before the Great Fire of London. Not a great couple of years for London). I think I would have enjoyed this slice-of-life book regardless, especially since it's about a historical event I'm not super familiar with, but the timing is kind of crazy. I would be reading about quarantining measures, and how some people kept trying to work instead of hunkering down because otherwise they would starve, and I'd look at the book and think...was this a trick? Was this written last month? Anyway, if you like history and don't mind 16th century writing style and a wee bit of repetition (that's what skimming is for!) It's definitely an interesting read.
At first, I wasn't sure I was going to like Kitchens of the Great Midwest by J. Ryan Stradal. The multiple shifts in perspective was a little bit jarring, and there's some real heart wrenching stuff that happens at the beginning of the novel. But it sucked me in and ultimately, I ended up loving it. The narrative meanders and weaves its way among lots of different characters, all connected in some way to food, and to endearingly weird teenager-turned-chef prodigy Eva Thorvald. It's sad, funny, heartbreaking, hopeful, sarcastic, and surprising. The characterization was so well done, especially considering how little relative page time each character got, and the setting was vivid and basically another character. As a Midwestern girl myself, I particularly appreciated that.
Lizzy & Jane by Katherine Reay is a story about two estranged sisters, one of whom is a successful chef who is trying to rediscover her joy and passion for cooking, the other a successful businesswoman, wife, and mom who is fighting cancer. There's a sweet love story as well, but the main plot revolves around the two sisters and their attempt to rebuild their relationship. It was a quick read, but had surprising depth.
Currently on my reading list is the book Between the World and Me by Ta-Nehisi Coates and another J. Ryan Stradal book, Lager Queen of Minnesota.
What I'm watching...
Has anyone else watched The English Game yet? It's a Netflix series by the creator of Downton Abbey about the origins of modern soccer (or, football, to get technical). It focuses on the emergence of working class teams, the growth of the game, and the much-resisted (among the upper classes) move toward recruiting and paying players. It's only six episodes, but it packs so much into those episodes! There were plot points I anticipated, and quite a few that surprised me. I highly recommend it if you like period dramas at all, and even if you don't.
Jeremy and I had been catching up on a show on Hulu, so I had been seeing advertisements for Zoey's Extraordinary Playlist for weeks, and finally decided one night to give it a try. It's such a delight! It's weird and funny and silly, but with a lot of heart. The premise: after experiencing an earthquake in the middle of having an MRI, Zoey starts hearing people sing (and dance) their innermost feelings, and starts trying to help the people who sing to her (it kind of reminds me a little bit of that 90s show Early Edition. Anyone else watch that one back in the day?). Like I said, it sounds kind of silly, but I'm really enjoying it.
Upload was another show I kept hearing about (this time on Amazon Prime). In the near future (2030, I think), people have developed the technology to upload their consciousness into a virtual afterlife (if you can afford it, of course), a way to sort of live forever. It's a light and funny show, but touches on some deep themes like mortality, love, connection, family. There's a bit of a mystery too, and all packed in 30 minute shows. I will admit that I started watching it on a night Jeremy was out of town, and ended up watching almost the entire season (10 episodes) in one night. And yes, I did kind of regretted the late night the next day.
What I'm grateful for…
Summer weather has come to Houston with a vengeance (although this week has actually had some incredible weather), so today I'm grateful for our central air conditioning and the convenience of living with someone who can repair the A/C when it goes out at 10 pm.
A little inspiration...
Excerpt from "Traveling at Home"
By Wendell Berry
Even in a country you know by heart
It's hard to go the same way twice.
The life of the going changes.
The chances change and make a new way.
Know therefore today, and lay it on your heart, that the Lord is God in heaven above and on earth beneath; there is no other.
Happy Summer, everyone! Stay hydrated, wear your sunscreen, and love your neighbor well.
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Popcorn and Video Games
Go Team!
The Pen is Mightier than the Sword
Geology Rocks!
Feasts!
Supper with Friends
Rugby & Gingerbread
Geek Chic HQ
Space monkey reporting for duty!
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Come with me, Cupcake
May 14, 2013 by Eve England
2013 has already been an exciting year for those with an interest in all things SPACE.
On February 15, the Russians were dazzled by an epic superbolide meteor, which rather ironically coincided with the day on which the people of Earth heaved a mighty sigh of relief as they watched asteroid DA14, a.k.a, a "global killer", passing uncomfortably close between their planet and their moon, thus narrowly avoiding a cataclysmic impact. Later in the year, Ison, a sun-grazing comet, promises to be a shooting-star spectacular, visible to the naked eye by the end of November, assuming it survives its journey towards the Sun, that is!
The concept of space as our final frontier has also been at the forefront of scientific discourse this week, as veteran astronaut, Buzz Aldrin, proclaimed (in a rather Kennedy-esq mission statement): "I believe this nation should commit itself, within two decades, to commencing American permanence on the planet Mars." This declaration has been made at a time where enthusiasm for exploration beyond our planet continues to ebb, and NASA's abandonment of the shuttle programme, along with the mounting criticism of the $multi-billion cost of maintaining the scientific endeavours on board the International Space Station have done little to appease public apathy towards such pursuits.
Commander Chris Hadfield, the Canadian astronaut who hitched a ride home from the ISS on a Soyuz rocket last night, shares Aldrin's aspirations for future interplanetary travel, by asserting that "we will go to the Moon and we will go to Mars…and to see what asteroids and comets are made of". But Hadfield is at odds with Aldrin in so far as the former pointedly reminds us that "we're not going to do it tomorrow and we're not going to do it because it titillates the nerve endings. We're going to do it because it's a natural human progression."
Hadfield and the other astronauts on the Space Station are tasked with developing a greater understanding of life's ability to sustain itself in the hostile environment of space, without which, we would not even survive a trip to Mars, yet alone presuming to permanently settle there.
The political and ideological motivations driving the extraordinary technological advancement witnessed during the 1960s were unprecedented, and never again are we likely to witness such a frenzied obsession with space travel. However, Cmdr Hadfield has sought to re-kindle mankind's fascination with space by embracing the social media revolution to reach out to a brand new generation of space enthusiasts. By taking daily tweet-sized steps to share his experience of life in space with us, he has become the most recognisable astronaut since the Apollo days.
The movie industry has also played its part in supporting Aldrin and Hadfield's ambitions, with Thursday's release of Star Trek's 12th instalment (or 2nd, depending on whether you choose to ignore the pre 2009 chronicles) having been eagerly anticipated by fans, and non-fans alike, after the 11th (or 1st) cemented J.J.Abrahms' reputation as a tired franchise resurrection master. Into Darkness' opening sequence sets the tone for the rest of the picture, as we observe Kirk finding himself at odds with the Federation's sacrosanct Prime Directive. The scene ends as the iconic USS Enterprise blasts off above a neutralised volcano, leaving a primitive alien tribe in awe of Starfleet's flagship vessel.
As was the case with the 2009 release, the audience is treated to non-stop, over-the-top, and breathtaking action sequences, all illuminated by Abrahm's signature use of delightful lens glare, once again assuring us that the future may not be so dark after all. But Cumberbatch's Harrison does all that he can to extinguish the optimism introduced in the last movie, and is undoubtedly an improvement on Nero: An unnerving villain, whose indiscriminate acts of violence on London and San Francisco capture the fears of a post 9/11 society. The references to the autonomous intelligence agency, Section 31 (first introduced into the Star Trek canon by Deep Space Nine) highlights the disquietude brewing within the seemingly harmonised Starfleet high command, and seeks to further underpin the movie's emphasis on how, in certain instances, acting outside of the normal constraints of ethical protocols is necessary to preserve and protect civilised society.
Despite its brooding undercurrents, Into Darkness captures the essence of the space opera genre by continuing to replicate the original's charm: it wouldn't be Star Trek without the delectable Kirk/Spock bromance, and the old school wittiness of the classic once liners of sci-fi's most recognised crew. A reprocessed Khan it may be, but there is no question that Into Darkness has taken the Star Trek franchise to a new level, and we can only hope that the brief visit to Kronos, and the ruckus that takes place there, is a sign of things to come.
To explore strange new worlds
Inspired by the Star Trek end-credit sequence, I decided to create a cup-cake solar-system for my father in-law's 60th birthday/retirement party that was held on the Isle of Wight this weekend. As a published physicist, this seemed like a fitting contribution to the celebrations.
The cupcakes were made using the Hummingbird Bakery chocolate cupcake recipe, and these were topped with a nebula of chocolate, vanilla and cream cheese frosting, sprinkled with dark matter space dust.
The celestial bodies were formed using marzipan, and represent the main features of our solar system from the Sun, all the way out to Pluto and the forgotten dwarf planets.
You will see that Ison makes a special guest appearance as it passes Jupiter!
To Nick and Sue – wishing you all the best as you boldly go into that strange new world called retirement!
This entry was posted in Feasts!, Space Monkey and tagged Buzz Aldrin, Commander Chris Hadfield, DA14, geek chic, Hummingbird Bakery, Ison, ISS, Khan, Mars, NASA, nerd tears, Planets, Section 31, Solar System Cupcakes, soyuz, Star Trek Into Darkness. Bookmark the permalink.
#RWC2019 GingerBreadOfHeaven On Tour – Japan Trip Highlights
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Great Scott!! It's Back to the Future Day, and it didn't take 1.21GW to get us here!
Stunning sunset drive home this evening via the #BreconBeacons Always a pleasure to see the wild ponies...… twitter.com/i/web/status/1… 2 hours ago
Fab trip to #PowisCastle this afternoon! @NTPowisCastle https://t.co/mN7fQUwxW7 6 hours ago
RT @andNickMullins: Confirmation. Saracens relegated. They'll be playing in the Championship next season. Goodness me. What a mess. https:/… 19 hours ago
RT @btsportrugby: Premiership Rugby have announced Saracens will be relegated at the end of the 19/20 season. https://t.co/wi0jGo4EZ3 1 day ago
Finally got around to start editing our #GOTTerritory pictures from the summer. It's taking a bit of time... such… twitter.com/i/web/status/1… 1 day ago
Super Bowl XLVIII
It's crunch time!
International Series –
WembleyOctober 31st, 2014


Vantiv Executives to Present at Three Upcoming Financial Conferences
CINCINNATI, May 11, 2017 /PRNewswire/ -- Vantiv, Inc. (NYSE: VNTV), a leading provider of payments processing services and related technology solutions for merchants and financial institutions, announced today that its executives will attend and present at three upcoming conferences.
On Tuesday, May 23, Charles Drucker, president and chief executive officer, will speak at 10:00 a.m. ET at the J.P. Morgan 45th Annual Global Technology, Media, and Telecom Conference, being held at the Westin Copley Place in Boston.
On Thursday, June 1, Matt Taylor, group president, Integrated Payments and Emerging Channels at Vantiv, will speak at 9:30 a.m. ET at the Cowen Future of Payments & Commerce Forum, being held at the Lotte New York Palace Hotel in New York City.
On Tuesday, June 6, Charles Drucker, president and chief executive officer, will present at 9:05 a.m. ET at the Baird 2017 Global Consumer, Technology & Services Conference being held at the InterContinental New York Barclay in New York City.
Each presentation will be webcast live and can be accessed by visiting the investor section of Vantiv's website at http://investors.vantiv.com. The webcasts will be archived and available for replay following each event.
For more information about Vantiv, please visit vantiv.com or on Twitter, Facebook, LinkedIn, Google+ and YouTube.
ABOUT VANTIV
Vantiv, Inc. (NYSE: VNTV) is a leading payment processor differentiated by an integrated technology platform. Vantiv offers a comprehensive suite of traditional and innovative payment processing and technology solutions to merchants and financial institutions of all sizes, enabling them to address their payment processing needs through a single provider. We build strong relationships with our customers, helping them become more efficient, more secure and more successful. Vantiv is the largest merchant acquirer and the largest PIN debit acquirer based on number of transactions in the U.S. The company's growth strategy includes expanding further into high-growth channels and verticals, including integrated payments, eCommerce, and merchant bank. Visit us at the new www.vantiv.com, or follow us on Twitter, Facebook, LinkedIn, Google+ and YouTube.
© 2017 Vantiv, LLC. All Rights Reserved. All trademarks, service marks and trade names referenced herein are the property of their respective owners. Vantiv and other Vantiv products and services mentioned herein as well as their respective logos are registered trademarks or trademarks of Vantiv, LLC in the U.S. and other countries.
Nathan Rozof, CFA
IR@vantiv.com
Andrew Ciafardini
External Affairs & Corporate Communications
andrew.ciafardini@vantiv.com
To view the original version on PR Newswire, visit:http://www.prnewswire.com/news-releases/vantiv-executives-to-present-at-three-upcoming-financial-conferences-300456497.html
SOURCE Vantiv, Inc.


The Silas Bartsch School Site Council is made up of teachers, parents, administrators, and interested community members who work together to develop and monitor a school's improvement plan. Their primary role is to oversee the academic planning process to ensure that the needs of all students are specifically addressed in the School pLan for Student Achievement (SPSA).
SSC Roles and Responsibilities
The Silas Bartsch School Site Council (SSC) is a group of teachers, parents, administrators, and interested community members who are elected by their colleagues and/or peers to work together to develop and monitor our school's improvement plan. Their primary role is to oversee the academic planning process to ensure that the needs of all students are specifically addressed in the School Plan for Student Achievement (SPSA).
Measure the effectiveness of improvement strategies at our school
Seek input from school advisory committees such as the English Language Advisory Committee (ELAC)
Review and analyze student academic information
Revise improvement strategies and expenditures
Develop the approve the School Plan for Student Achievement (SPSA), and monitor implementation of the SPSA
Provide input on District Local Control and Accountability Plan (LCAP)
Represent our school and participate in the District Advisory Committee (DAC)
Parent or community membership is equal to school staff membership.
Classroom teachers must be in the majority of the staff side.
"Other School Personnel" includes: Other administrative positions, other non-classroom teacher positions, and all site classified personnel.
Smallest council is composed of 10 members.
Four School Site Council (SSC) meetings are conducted annually.
Tuesday, September 27, 2022: 5:00pm in the Student Center
SSC elections
Complete SSC training
Review SSC roles and responsibilities
Review and approve School Parent Compact
Review School Safety Plan
Review bylaws and revise as needed
Tuesday, January 24, 2023: 5:00pm in the Student Center
Review student achievement data / test results
Discuss data analysis and student needs
Review current School Plan for Student Achievement (SPSA)
Title I Parent and Family Engagement Policy
Tuesday, March 7, 2023: 5:00pm in the Student Center
Review Dress Code
Review latest student achievement data and needs assessment
Establish SPSA writing team and timeline
Discuss SPSA revisions for next year
Tuesday, May 16, 2023: 5:00pm in the Student Center
Review/discuss SPSA and budget for next school year
Approve SPSA and budget for next school year


Home Urgent Needs Help Us About Care Uk Contacts
Sending containers of aid to refugees
Care UK Charity collects and distributes practical items and medical aid to people in need. End of line, unwanted or excess stock items are sorted, packed and loaded onto 40 ft containers at our Northwich Depot, leaving for the refugees in Syria, Greece, or the Greek Islands.
Helping people who have almost nothing
Care UK Charity's Homes Team also assists refugees who have settled in the UK and local people referred by Social Services or other charities. These people often have almost nothing available to them to set up home or conduct their lives. The Care UK Homes Team are always in urgent need of furniture, household items and soft furnishings, in order to create a comfortable home from home.
Recycling unwanted stock
Are you a manufacturer or stockist of any of the items on our most wanted list? Do you have stocks that are nearly out of date, or no longer current? Could you donate them to us? You would be fulfilling your corporate social responsibility in terms of recycling, and clearing space in your storage facilities all at the same time.
More importantly, you will be helping people in need, families with children trying to survive in horrendous conditions both here in the UK and overseas.
Thank You from Care UK Charity
Huge thanks to Procter & Gamble who came to the rescue again, sending us 23 pallets of mixed hygiene and nappies. We were all totally blown away.
pg.co.uk
Most urgently needed items
These are our most urgent needs this month. If you can donate any sort of practical or essential items, hygiene product, or medical items that we have not listed PLEASE let us know as we probably need them too. This is just the most urgent list. Please help make a difference to people's lives.
Pushchairs/strollers
Dishwashing liquid
Raincoats/ponchos
Socks (new)
Sweatshirts (Small/Med Men's)
Trousers (adult, teenager, children)
Underwear-new (adult's/children's)
Disability Aids
Beans (Tinned)
Dried Food (pasta, rice, couscous etc)
Tuna (Tinned)
Carpet pieces (new)
Craft/Art Supplies
Suitcases/backpacks
Lice lotion
Roll-on deodorant
Located in the North West of England
Care UK Charity was set up in Cheshire, you will find our main donation and sorting station in Northwich, although we are expanding and now have additional sorting stations in Manchester and North Wales. We welcome volunteers from across the region.
Donation Stations
Our main donation station and sorting centre is located at 40 Chester Way, Northwich, Cheshire, CW9 5JA Other sorting centres are located in Chorlton, Manchester and Buckley, North Wales.
Our retail outlets are at Unit 17/18 Cockhedge Shopping Park, Warrington WA1 2QQ phone 01925 418793 and 40 Chester Way, Northwich CW9 5JA phone 01606 43865
Volunteer Sessions
For opening times and volunteer sessions see the Care UK Charity Facebook page


Home > For Media > Press Releases > NMMC-West Point Honors Wofford as Employee of the Quarter
NMMC-West Point Honors Wofford as Employee of the Quarter
WEST POINT, Miss.—Joy Wofford, RN, has been selected as the most recent Employee of the Quarter at North Mississippi Medical Center-West Point.
A graduate of West Point High School, Wofford attained her licensed practical nursing certification and joined the NMMC-West Point staff in 1994. She earned her associate degree in nursing from Mississippi University for Women in 1995. In 1999 she transferred to the hospital's Emergency Department. She is certified in Advanced Cardiac Life Support, Pediatric Advanced Life Support and the Trauma Nurse Core Curriculum.
In her nominations, coworkers praised Wofford for always putting patients' well-being first and for providing "extras" for patients. "Joy purchases toys, gifts, socks, clothing and other items to have in the ER for children and families when needed," one nomination states. Another wrote, "Joy has been a part of this community for all of her life and shows compassion through her work." She was also cited as a hard worker who is always punctual and continually learning.
"What I love most about my job is the people I work with. We are a family," Wofford said. "We all have a part in making a positive impact or difference in someone's life. NMMC-West Point has the most amazing and caring staff."
She is a member of First Baptist Church in West Point.


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Council Fly-tipping Costs 'Double'.
Posted on September 5, 2011 by Mike Rigby
Bishops Lydeard Parish Councillor, Mike Rigby, has slammed Somerset Councils, including Tory-run Taunton Deane for the cuts made to the Council's waste recycling centres. Reduced opening hours and tipping charges were brought in at all Somerset Waste Recycling Centres in April. Many people, including Cllr Rigby, were concerned that these changes would lead to increased fly-tipping. John Williams, Conservative leader of Taunton Deane Borough Council said at the time, "It's too early to see if there's going to be fly-tipping."
Under a freedom of information request, Cllr Rigby has discovered that the cost of dealing with fly-tipping in the first quarter since the changes were introduced is almost half of the cost of the whole of last year's clean-up costs. "It's clear from all the anecdotal evidence, we've been receiving that fly-tipping is on the increase and now Taunton Deane's own figures prove this.
It's time Taunton Deane's Conservative administration woke up to the evidence and reversed these hugely counter-productive changes." Waste Recycling Sites (once known as Civic Amenity Sites, taking their name from the Act that introduced them) were introduced in the 1960s in order to combat fly-tipping. "It's not rocket science to understand that cutting access to such facilities is going to increase fly-tipping. Taunton Deane appears to have forgotten why these sites were introduced in the first place," added Cllr Rigby. "Even Communities Secretary Eric Pickles, no lover of Council spending, said in April, "Charging for using recycling centres and the local dump will create perverse incentives for fly-tipping, harming the local environment and diverting tax-payers money into clearing up". Cllr Rigby agreed with those sentiments, "Now we have the evidence to show that at current rates, the cost of cleaning up fly-tipping in Taunton Deane will almost double as a result of this short-sighted policy. Add to that, the environmental damage and harm to wildlife and enjoyment of the countryside that results from fly-tipped waste and it's clear that the policy must change."
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