focus add arrayexpress E-MTAB-6879

.gitattributes

@@ -243,3 +243,4 @@ saved_model/**/* filter=lfs diff=lfs merge=lfs -text
 脓毒症/单细胞组/arrayexpress_E-MTAB-9444/Notum3.txt filter=lfs diff=lfs merge=lfs -text
 脓毒症/单细胞组/arrayexpress_E-MTAB-9444/Notum4.txt filter=lfs diff=lfs merge=lfs -text
 脓毒症/单细胞组/arrayexpress_E-MTAB-8503/Cell_Count_Matrix.txt filter=lfs diff=lfs merge=lfs -text
+脓毒症/单细胞组/arrayexpress_E-MTAB-6879/all_samples_normalized_readcounts.txt filter=lfs diff=lfs merge=lfs -text

脓毒症/单细胞组/arrayexpress_E-MTAB-6879/E-MTAB-6879.idf.txt

@@ -0,0 +1,70 @@
+MAGE-TAB Version	1.1
+Investigation Title	Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level
+
+Experimental Design	cell type comparison design
+Experimental Design Term Source REF	EFO
+Experimental Design Term Accession Number	EFO:0001745
+
+Experimental Factor Name	single cell identifier	phenotype
+Experimental Factor Type	single cell identifier	phenotype
+Experimental Factor Term Source REF
+Experimental Factor Term Accession Number
+
+Person Last Name	Han	Koo
+Person First Name	Seungmin	Bon-Kyoung
+Person Mid Initials
+Person Email	sh906@cam.ac.uk	bonkyoung.koo@imba.oeaw.ac.at
+Person Phone
+Person Fax
+Person Affiliation	WT-MRC Cambridge Stem Cell Institute, The University of Cambridge	Institute of Molecular Biotechnology
+Person Address	Tennis Court Road, Cambridge, CB2 1QR, UK	Vienna, 1030, Austria
+Person Roles	submitter	investigator
+Person Roles Term Source REF
+Person Roles Term Accession Number
+
+Quality Control Type
+Quality Control Term Source REF
+Quality Control Term Accession Number
+
+Replicate Type
+Replicate Term Source REF
+Replicate Term Accession Number
+
+Normalization Type
+Normalization Term Source REF
+Normalization Term Accession Number
+
+Date of Experiment	2017-06-05
+Public Release Date	2019-08-14
+
+PubMed ID
+Publication DOI
+Publication Author List	Fink, J.; Han, S.; Jörg, D.; Merker, S.R.; Lee, E.; Yum, M.K.; Lee, J.H.; Josserand, M.; Trendafilova, T.; Andersson-Rolf, A.; Dabrowska, C.; Kim, H.; Mort, R.L.; Jackson, I.J.; Basak, O.; Clevers, H.; Marioni, J.C.; Stange, D.E.; Kim, J.K.; Simons, B.D.; Koo, B.K.
+Publication Title	Defining the identity and dynamics of adult gastric isthmus stem cells
+Publication Status	in preparation
+Publication Status Term Source REF
+Publication Status Term Accession Number
+
+Experiment Description	The purpose of this single cell experiment is to compare and characterize at molecular level actively cycling stem cells in the isthmus and quiescent stem cells in the base of the mouse stomach corpus. The lineage tracing data from Stmn1-CreERT2 shows that Stmn1+ cells in the corpus isthmus include fast dividing isthmus stem cells with long-term potency. On the other hand, chief cells including Lgr5+ subpopulation can play as quiescent stem cells in the base that are largely quiescent in homeostasis, but are activated upon injury. The isthmus stem cells and chief cells are isolated by Stmn1 and Pgc, respectively, and were subject to single cell RNA-seq experiment.
+
+Protocol Name	P-MTAB-75797	P-MTAB-75798	P-MTAB-75799	P-MTAB-75800	P-MTAB-75802	P-MTAB-75801
+Protocol Type	sample collection protocol	nucleic acid extraction protocol	nucleic acid library construction protocol	nucleic acid sequencing protocol	normalization data transformation protocol	high throughput sequence alignment protocol
+Protocol Term Source REF	EFO	EFO	EFO	EFO	EFO	EFO
+Protocol Term Accession Number	EFO_0005518	EFO_0002944	EFO_0004184	EFO_0004170	EFO_0003816	EFO_0004917
+Protocol Description	The mouse stomach corpus was dissected and the tissue was dissociated into single-cell suspension by the incubation with Dispase ‖ (45U.ml). Then the single cells were sorted into 384 well plates containing lysis buffer by BD INFLUX. Spike-in RNA from the External RNA Controls Consortium (ERCC) (Ambion, Life Technologies) was added to each well in 1:640,000 dilution. Stomach cell sorting was performed by the WT Sanger Institute Flow Cytometry Facility for single cell RNA-seq data generation. Stomachs were prepared by carefully separating the corpus from the forestomach and pylorus. Corpus tissue was divided into 4 pieces of similar size and incubated in 4 mL dissociation solution (45 U/mL Dispase II, Thermo Fisher Scientific; 0.6 mg/mL Pancreatin, Sigma; 1x Penicillin/Streptomycin in DMEM high glucose, HEPES, no phenol red, Thermo Fisher Scientific) at 37 °C with shaking at 270 rpm. After dissociation the solution becomes cloudy and corpus fragments appear more transparent. All subsequent steps were then performed on ice. To avoid cell loss due to cell adhesion to pipette or tube walls, all pipettes and tubes used for pipetting the cell suspension were pre-incubated with either DMEM or PBS containing FBS. To further disrupt the tissue, the cell suspension, including corpus pieces, was pipetted up and down several times using a 10 mL pipette. The cell suspension without remaining corpus pieces was then transferred to a 15 mL Falcon tube. The corpus pieces and the dissociation tube were then washed twice with 5 mL DMEM + 10% FBS and the wash solutions combined with the cell suspension for inactivation of the dissociation reaction. Cells were centrifuged at 300 g for 5 min and resuspended in 1% FBS in PBS. The cell suspension was filtered through a 100 µm cell strainer into a pre-coated 15 ml tube. The tube and filters were washed twice with 1 mL of 1% FBS in PBS and the cell suspension was then centrifuged a second time at 300 g for 5 min. The supernatant was removed and the cell pellet was resuspended in 100 µL of antibody mix (1% FBS; 10 U/mL DNAse, Promega; 1:125 Alexa Fluor® 647-conjugated anti-mouse/human CD324 (E-Cadherin) antibody, BioLegend) and incubated for 1 h on ice. Cells were washed with 3 mL 1% FBS in PBS and filtered once more if clumps could be observed. After centrifugation at 300 g for 5 min the cell pellet was resuspended in 1 mL 1% FBS and 10 U/mL DNAse in PBS for sorting. BD INFLUX systems were used for sorting in the WT Sanger Institute Flow Cytometry Facility. After the viable cells were gated at first, the cell were subject to the second gating to select only singlet cells. Then the third gating was to select E-Cadherin+ epithelial cells by Alexa 647. The fourth gating was performed to select Stmn1+ cells by eGFP or Pgc+ cells by dsRed depending of the target cells we want to isolate in different single cell data generation.	Each well of the 384 well plate contained 2.3 ul of lysis buffer with RNAse inhibitor (Ambion) in a 0.2% (v/v) Triton X-100 solution.	Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 25 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina).	Libraries were sequenced on an Illumina HiSeq 2500 in rapid run to obtain 100 bp paired-end reads.	Mapped reads were normalized using pool-based sized factors that were featured in the scran (v1.6.9) package of R, which allows normalization of sparse scRNA-seq data. The normalized counts were then log2-transfromed with a pseudocount count of 1.	Paired-end reads of Stmn+ single cells were mapped to the Mus musculus genome (GRCm38) using STAR (v2.5.2b) with default parameters. Splice junctions in reads were detected with the help of a GTF file of GRCm38 provided by Ensembl (release 90). Uniquely mapped reads were counted for each gene using htseq-count (v0.7.2). We removed poor-quality cells that have greater than 10% reads mapped to mitochondrial-encoded genes and greater than 97% genes expressed below a detection limit of 5 read counts.
+Protocol Parameters
+Protocol Hardware				Illumina HiSeq 2500
+Protocol Software
+Protocol Contact
+
+Term Source Name	EFO	ArrayExpress
+Term Source File	http://www.ebi.ac.uk/efo/	http://www.ebi.ac.uk/arrayexpress/
+Term Source Version	2.38
+
+SDRF File	E-MTAB-6879.sdrf.txt
+Comment [Submitted Name]	Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level
+Comment [SecondaryAccession]	ERP109352
+Comment [SequenceDataURI]	http://www.ebi.ac.uk/ena/data/view/ERR2635265-ERR2637184
+Comment [AEExperimentType]	RNA-seq of coding RNA from single cells
+Comment[ArrayExpressAccession]	E-MTAB-6879
+Comment [RelatedExperiment]	E-MTAB-6850

脓毒症/单细胞组/arrayexpress_E-MTAB-6879/all_samples_normalized_readcounts.txt

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脓毒症/单细胞组/arrayexpress_E-MTAB-6879/biostudies.json

@@ -0,0 +1,1284 @@
+{
+  "accno": "E-MTAB-6879",
+  "attributes": [
+    {
+      "name": "Title",
+      "value": "Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level"
+    },
+    {
+      "name": "ReleaseDate",
+      "value": "2019-08-14"
+    },
+    {
+      "name": "RootPath",
+      "value": "E-MTAB-6879"
+    },
+    {
+      "name": "AttachTo",
+      "value": "ArrayExpress"
+    }
+  ],
+  "section": {
+    "accno": "s-E-MTAB-6879",
+    "type": "Study",
+    "attributes": [
+      {
+        "name": "Title",
+        "value": "Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level"
+      },
+      {
+        "name": "Study type",
+        "value": "RNA-seq of coding RNA from single cells",
+        "valqual": [
+          {
+            "name": "Ontology",
+            "value": "EFO"
+          },
+          {
+            "name": "TermId",
+            "value": "EFO_0005684"
+          }
+        ]
+      },
+      {
+        "name": "Organism",
+        "value": "Mus musculus"
+      },
+      {
+        "name": "Description",
+        "value": "The purpose of this single cell experiment is to compare and characterize at molecular level actively cycling stem cells in the isthmus and quiescent stem cells in the base of the mouse stomach corpus. The lineage tracing data from Stmn1-CreERT2 shows that Stmn1+ cells in the corpus isthmus include fast dividing isthmus stem cells with long-term potency. On the other hand, chief cells including Lgr5+ subpopulation can play as quiescent stem cells in the base that are largely quiescent in homeostasis, but are activated upon injury. The isthmus stem cells and chief cells are isolated by Stmn1 and Pgc, respectively, and were subject to single cell RNA-seq experiment."
+      }
+    ],
+    "links": [
+      [
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+          "url": "E-MTAB-6850",
+          "attributes": [
+            {
+              "name": "Type",
+              "value": "Biostudies"
+            }
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+          "attributes": [
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+            },
+            {
+              "name": "Hardware"
+            }
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+            },
+            {
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+            }
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+          "type": "Protocols",
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+              "name": "Name",
+              "value": "P-MTAB-75799"
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+              "value": "Preamplification was performed in a total volume of 27 ul that contained 13.5 ul of HiFi Hotstart ReadyMix (2x; KAPA Biosystems) and 0.1 uM of IS PCR primer (Sigma-Aldrich). After 25 cycles of amplification, samples were cleaned with 80% (v/v) of Ampure beads (Beckman Coulter). Sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina)."
+            },
+            {
+              "name": "Hardware"
+            }
+          ]
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+          "type": "Protocols",
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+              "name": "Name",
+              "value": "P-MTAB-75800"
+            },
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+              "name": "Type",
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+                  "value": "EFO_0004170"
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+              "value": "Libraries were sequenced on an Illumina HiSeq 2500 in rapid run to obtain 100 bp paired-end reads."
+            },
+            {
+              "name": "Hardware",
+              "value": "Illumina HiSeq 2500"
+            }
+          ]
+        },
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+          "accno": "P-MTAB-75802",
+          "type": "Protocols",
+          "attributes": [
+            {
+              "name": "Name",
+              "value": "P-MTAB-75802"
+            },
+            {
+              "name": "Type",
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+              "valqual": [
+                {
+                  "name": "Ontology",
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+            },
+            {
+              "name": "Hardware"
+            }
+          ]
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+          "accno": "P-MTAB-75801",
+          "type": "Protocols",
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+            {
+              "name": "Name",
+              "value": "P-MTAB-75801"
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+                  "value": "EFO"
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+                  "value": "EFO_0004917"
+                }
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+            },
+            {
+              "name": "Hardware"
+            }
+          ]
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+      {
+        "type": "Author",
+        "attributes": [
+          {
+            "name": "Name",
+            "value": "Seungmin Han"
+          },
+          {
+            "name": "Email",
+            "value": "sh906@cam.ac.uk"
+          },
+          {
+            "name": "Role",
+            "value": "submitter"
+          },
+          {
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+            "value": "o1",
+            "reference": true
+          }
+        ]
+      },
+      {
+        "type": "Author",
+        "attributes": [
+          {
+            "name": "Name",
+            "value": "Bon-Kyoung Koo"
+          },
+          {
+            "name": "Email",
+            "value": "bonkyoung.koo@imba.oeaw.ac.at"
+          },
+          {
+            "name": "Role",
+            "value": "investigator"
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+            "name": "affiliation",
+            "value": "o2",
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+      {
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+        "type": "Organization",
+        "attributes": [
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+            "value": "WT-MRC Cambridge Stem Cell Institute, The University of Cambridge"
+          },
+          {
+            "name": "Address",
+            "value": "Tennis Court Road, Cambridge, CB2 1QR, UK"
+          }
+        ]
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+      {
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+        "type": "Organization",
+        "attributes": [
+          {
+            "name": "Name",
+            "value": "Institute of Molecular Biotechnology"
+          },
+          {
+            "name": "Address",
+            "value": "Vienna, 1030, Austria"
+          }
+        ]
+      },
+      {
+        "type": "Publication",
+        "attributes": [
+          {
+            "name": "Title",
+            "value": "Defining the identity and dynamics of adult gastric isthmus stem cells"
+          },
+          {
+            "name": "Authors",
+            "value": "Fink, J.; Han, S.; Jörg, D.; Merker, S.R.; Lee, E.; Yum, M.K.; Lee, J.H.; Josserand, M.; Trendafilova, T.; Andersson-Rolf, A.; Dabrowska, C.; Kim, H.; Mort, R.L.; Jackson, I.J.; Basak, O.; Clevers, H.; Marioni, J.C.; Stange, D.E.; Kim, J.K.; Simons, B.D.; Koo, B.K."
+          },
+          {
+            "name": "Status",
+            "value": "in preparation"
+          }
+        ]
+      },
+      {
+        "accno": "s-samples-factors-E-MTAB-6879",
+        "type": "Samples",
+        "attributes": [
+          {
+            "name": "Sample count",
+            "value": "1920"
+          },
+          {
+            "name": "Experimental Designs",
+            "value": "cell type comparison design",
+            "valqual": [
+              {
+                "name": "TermId",
+                "value": "EFO:0001745"
+              }
+            ]
+          },
+          {
+            "name": "Experimental Factors",
+            "value": "single cell identifier",
+            "valqual": [
+              {
+                "name": "TermName",
+                "value": "single cell identifier"
+              }
+            ]
+          },
+          {
+            "name": "Experimental Factors",
+            "value": "phenotype",
+            "valqual": [
+              {
+                "name": "TermName",
+                "value": "phenotype"
+              }
+            ]
+          }
+        ],
+        "subsections": [
+          {
+            "accno": "exp_factor-E-MTAB-6879",
+            "type": "Experimental Factors",
+            "attributes": [
+              {
+                "name": "Phenotype",
+                "value": "Stmn1 positive"
+              },
+              {
+                "name": "Phenotype",
+                "value": "Pgc positive"
+              },
+              {
+                "name": "Single cell identifier",
+                "value": "21196_232"
+              },
+              {
+                "name": "Single cell identifier",
+                "value": "21196_233"
+              },
+              {
+                "name": "Single cell identifier",
+                "value": "21196_234"
+              },
+              {
+                "name": "Single cell identifier",
+                "value": "... 1917 other values"
+              }
+            ],
+            "subsections": [
+              [
+                {
+                  "accno": "factors_0",
+                  "type": "Factors Table",
+                  "attributes": [
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "1152",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546680-ERS2547831"
+                        }
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+                    }
+                  ]
+                },
+                {
+                  "accno": "factors_1",
+                  "type": "Factors Table",
+                  "attributes": [
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+                      "name": "phenotype",
+                      "value": "Stmn1 positive"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "768",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547832,ERS2547833,ERS2547834,ERS2547835,ERS2547836,ERS2547837,ERS2547838,ERS2547839,ERS2547840,ERS2547841,ERS2547842,ERS2547843,ERS2547844,ERS2547845,ERS2547846,ERS2547847,ERS2547848,ERS2547849,ERS2547850,ERS2547851,ERS2547852,ERS2547853,ERS2547854,ERS2547855,ERS2547856,ERS2547857,ERS2547858,ERS2547859,ERS2547860,ERS2547861,ERS2547862,ERS2547863,ERS2547864,ERS2547865,ERS2547866,ERS2547867,ERS2547868,ERS2547869,ERS2547870,ERS2547871,ERS2547872,ERS2547873,ERS2547874,ERS2547875,ERS2547876,ERS2547877,ERS2547878,ERS2547879,ERS2547880,ERS2547881,ERS2547882,ERS2547883,ERS2547884,ERS2547885,ERS2547886,ERS2547887,ERS2547888,ERS2547889,ERS2547890,ERS2547891,ERS2547892,ERS2547893,ERS2547894,ERS2547895,ERS2547896,ERS2547897,ERS2547898,ERS2547899,ERS2547900,ERS2547901,ERS2547902,ERS2547903,ERS2547904,ERS2547905,ERS2547906,ERS2547907,ERS2547908,ERS2547909,ERS2547910,ERS2547911,ERS2547912,ERS2547913,ERS2547914,ERS2547915,ERS2547916,ERS2547917,ERS2547918,ERS2547919,ERS2547920,ERS2547921,ERS2547922,ERS2547923,ERS2547924,ERS2547925,ERS2547926,ERS2547927,ERS2547928,ERS2547929,ERS2547930,ERS2547931,ERS2547932,ERS2547933,ERS2547934,ERS2547935,ERS2547936,ERS2547937,ERS2547938,ERS2547939,ERS2547940,ERS2547941,ERS2547942,ERS2547943,ERS2547944,ERS2547945,ERS2547946,ERS2547947,ERS2547948,ERS2547949,ERS2547950,ERS2547951,ERS2547952,ERS2547953,ERS2547954,ERS2547955,ERS2547956,ERS2547957,ERS2547958,ERS2547959,ERS2547960,ERS2547961,ERS2547962,ERS2547963,ERS2547964,ERS2547965,ERS2547966,ERS2547967,ERS2547968,ERS2547969,ERS2547970,ERS2547971,ERS2547972,ERS2547973,ERS2547974,ERS2547975,ERS2547976,ERS2547977,ERS2547978,ERS2547979,ERS2547980,ERS2547981,ERS2547982,ERS2547983,ERS2547984,ERS2547985,ERS2547986,ERS2547987,ERS2547988,ERS2547989,ERS2547990,ERS2547991,ERS2547992,ERS2547993,ERS2547994,ERS2547995,ERS2547996,ERS2547997,ERS2547998,ERS2547999,ERS2548000,ERS2548001,ERS2548002,ERS2548003,ERS2548004,ERS2548005,ERS2548006,ERS2548007,ERS2548008,ERS2548009,ERS2548010,ERS2548011,ERS2548012,ERS2548013,ERS2548014,ERS2548015,ERS2548016,ERS2548017,ERS2548018,ERS2548019,ERS2548020,ERS2548021,ERS2548022,ERS2548023,ERS2548024,ERS2548026,ERS2548028,ERS2548031,ERS2548033,ERS2548035,ERS2548037,ERS2548039,ERS2548041,ERS2548043,ERS2548045,ERS2548048,ERS2548050,ERS2548052,ERS2548055,ERS2548057,ERS2548059,ERS2548061,ERS2548064,ERS2548066,ERS2548068,ERS2548071,ERS2548073,ERS2548075,ERS2548077,ERS2548079,ERS2548081,ERS2548083,ERS2548086,ERS2548089,ERS2548091,ERS2548094,ERS2548096,ERS2548099,ERS2548101,ERS2548104,ERS2548106,ERS2548108,ERS2548111,ERS2548113,ERS2548116,ERS2548119,ERS2548121,ERS2548123,ERS2548125,ERS2548126,ERS2548127,ERS2548128,ERS2548129,ERS2548130,ERS2548131,ERS2548132,ERS2548133,ERS2548134,ERS2548135,ERS2548136,ERS2548137,ERS2548138,ERS2548139,ERS2548140,ERS2548141,ERS2548142,ERS2548143,ERS2548144,ERS2548145,ERS2548146,ERS2548147,ERS2548148,ERS2548149,ERS2548150,ERS2548151,ERS2548152,ERS2548153,ERS2548154,ERS2548155,ERS2548156,ERS2548157,ERS2548158,ERS2548159,ERS2548160,ERS2548161,ERS2548162,ERS2548163,ERS2548164,ERS2548165,ERS2548166,ERS2548167,ERS2548168,ERS2548169,ERS2548170,ERS2548171,ERS2548172,ERS2548173,ERS2548174,ERS2548175,ERS2548176,ERS2548177,ERS2548178,ERS2548179,ERS2548180,ERS2548181,ERS2548182,ERS2548183,ERS2548184,ERS2548185,ERS2548186,ERS2548187,ERS2548188,ERS2548189,ERS2548190,ERS2548191,ERS2548192,ERS2548193,ERS2548194,ERS2548195,ERS2548196,ERS2548197,ERS2548198,ERS2548199,ERS2548200,ERS2548201,ERS2548202,ERS2548203,ERS2548204,ERS2548205,ERS2548206,ERS2548207,ERS2548208,ERS2548209,ERS2548210,ERS2548211,ERS2548212,ERS2548213,ERS2548214,ERS2548215,ERS2548216,ERS2548217,ERS2548218,ERS2548219,ERS2548220,ERS2548221,ERS2548222,ERS2548223,ERS2548224,ERS2548225,ERS2548226,ERS2548227,ERS2548228,ERS2548229,ERS2548230,ERS2548231,ERS2548232,ERS2548233,ERS2548234,ERS2548235,ERS2548236,ERS2548237,ERS2548238,ERS2548239,ERS2548240,ERS2548241,ERS2548242,ERS2548243,ERS2548244,ERS2548245,ERS2548246,ERS2548247,ERS2548248,ERS2548249,ERS2548250,ERS2548251,ERS2548252,ERS2548253,ERS2548254,ERS2548255,ERS2548256,ERS2548257,ERS2548258,ERS2548259,ERS2548260,ERS2548261,ERS2548262,ERS2548263,ERS2548264,ERS2548265,ERS2548266,ERS2548267,ERS2548268,ERS2548269,ERS2548270,ERS2548271,ERS2548272,ERS2548273,ERS2548274,ERS2548275,ERS2548276,ERS2548277,ERS2548278,ERS2548279,ERS2548280,ERS2548281,ERS2548282,ERS2548283,ERS2548284,ERS2548285,ERS2548286,ERS2548287,ERS2548288,ERS2548289,ERS2548290,ERS2548291,ERS2548292,ERS2548293,ERS2548294,ERS2548295,ERS2548296,ERS2548297,ERS2548298,ERS2548299,ERS2548300,ERS2548301,ERS2548302,ERS2548303,ERS2548304,ERS2548305,ERS2548306,ERS2548307,ERS2548308,ERS2548309,ERS2548310,ERS2548311,ERS2548312,ERS2548313,ERS2548314,ERS2548315,ERS2548316,ERS2548317,ERS2548318,ERS2548319,ERS2548320,ERS2548321,ERS2548322,ERS2548323,ERS2548324,ERS2548325,ERS2548326,ERS2548327,ERS2548328,ERS2548329,ERS2548330,ERS2548331,ERS2548332,ERS2548333,ERS2548334,ERS2548335,ERS2548336,ERS2548337,ERS2548338,ERS2548339,ERS2548340,ERS2548341,ERS2548342,ERS2548343,ERS2548344,ERS2548345,ERS2548346,ERS2548347,ERS2548348,ERS2548349,ERS2548350,ERS2548351,ERS2548352,ERS2548353,ERS2548354,ERS2548355,ERS2548356,ERS2548357,ERS2548358,ERS2548359,ERS2548360,ERS2548361,ERS2548362,ERS2548363,ERS2548364,ERS2548365,ERS2548366,ERS2548367,ERS2548368,ERS2548369,ERS2548370,ERS2548371,ERS2548372,ERS2548373,ERS2548374,ERS2548375,ERS2548376,ERS2548377,ERS2548378,ERS2548379,ERS2548380,ERS2548381,ERS2548382,ERS2548383,ERS2548384,ERS2548385,ERS2548386,ERS2548387,ERS2548388,ERS2548389,ERS2548390,ERS2548391,ERS2548392,ERS2548393,ERS2548394,ERS2548395,ERS2548396,ERS2548397,ERS2548398,ERS2548399,ERS2548400,ERS2548401,ERS2548402,ERS2548403,ERS2548404,ERS2548405,ERS2548406,ERS2548407,ERS2548408,ERS2548409,ERS2548410,ERS2548411,ERS2548412,ERS2548413,ERS2548414,ERS2548415,ERS2548416,ERS2548417,ERS2548418,ERS2548419,ERS2548420,ERS2548421,ERS2548422,ERS2548423,ERS2548424,ERS2548425,ERS2548426,ERS2548427,ERS2548428,ERS2548429,ERS2548430,ERS2548431,ERS2548432,ERS2548433,ERS2548434,ERS2548435,ERS2548436,ERS2548437,ERS2548438,ERS2548439,ERS2548440,ERS2548441,ERS2548442,ERS2548443,ERS2548444,ERS2548445,ERS2548446,ERS2548447,ERS2548448,ERS2548449,ERS2548450,ERS2548451,ERS2548452,ERS2548453,ERS2548454,ERS2548455,ERS2548456,ERS2548457,ERS2548458,ERS2548459,ERS2548460,ERS2548461,ERS2548462,ERS2548463,ERS2548464,ERS2548465,ERS2548466,ERS2548467,ERS2548468,ERS2548469,ERS2548470,ERS2548471,ERS2548472,ERS2548473,ERS2548474,ERS2548475,ERS2548476,ERS2548477,ERS2548478,ERS2548479,ERS2548480,ERS2548481,ERS2548482,ERS2548483,ERS2548484,ERS2548485,ERS2548486,ERS2548487,ERS2548488,ERS2548489,ERS2548490,ERS2548491,ERS2548492,ERS2548493,ERS2548494,ERS2548495,ERS2548496,ERS2548497,ERS2548498,ERS2548499,ERS2548500,ERS2548501,ERS2548502,ERS2548503,ERS2548504,ERS2548505,ERS2548506,ERS2548507,ERS2548508,ERS2548509,ERS2548510,ERS2548511,ERS2548512,ERS2548513,ERS2548514,ERS2548515,ERS2548516,ERS2548517,ERS2548518,ERS2548519,ERS2548520,ERS2548521,ERS2548522,ERS2548523,ERS2548524,ERS2548525,ERS2548526,ERS2548527,ERS2548528,ERS2548529,ERS2548530,ERS2548531,ERS2548532,ERS2548533,ERS2548534,ERS2548535,ERS2548536,ERS2548537,ERS2548538,ERS2548539,ERS2548540,ERS2548541,ERS2548542,ERS2548543,ERS2548544,ERS2548545,ERS2548546,ERS2548547,ERS2548548,ERS2548549,ERS2548550,ERS2548551,ERS2548552,ERS2548553,ERS2548554,ERS2548555,ERS2548556,ERS2548557,ERS2548558,ERS2548559,ERS2548560,ERS2548561,ERS2548562,ERS2548563,ERS2548564,ERS2548565,ERS2548566,ERS2548567,ERS2548568,ERS2548569,ERS2548570,ERS2548571,ERS2548572,ERS2548573,ERS2548574,ERS2548575,ERS2548576,ERS2548577,ERS2548578,ERS2548579,ERS2548580,ERS2548581,ERS2548582,ERS2548583,ERS2548584,ERS2548585,ERS2548586,ERS2548587,ERS2548588,ERS2548589,ERS2548590,ERS2548591,ERS2548592,ERS2548593,ERS2548594,ERS2548595,ERS2548596,ERS2548597,ERS2548598,ERS2548599,ERS2548600,ERS2548601,ERS2548602,ERS2548603,ERS2548604,ERS2548605,ERS2548606,ERS2548607,ERS2548608,ERS2548609,ERS2548610,ERS2548611,ERS2548612,ERS2548613,ERS2548614,ERS2548615,ERS2548616,ERS2548617,ERS2548618,ERS2548619,ERS2548620,ERS2548621,ERS2548622,ERS2548623,ERS2548624,ERS2548625,ERS2548626,ERS2548627,ERS2548628,ERS2548629,ERS2548630,ERS2548631,ERS2548632,ERS2548633,ERS2548634,ERS2548635,ERS2548636,ERS2548637,ERS2548638,ERS2548639,ERS2548640,ERS2548641,ERS2548642,ERS2548643,ERS2548644,ERS2548645,ERS2548646,ERS2548647,ERS2548648,ERS2548649,ERS2548650,ERS2548651,ERS2548652,ERS2548653,ERS2548654,ERS2548655,ERS2548656"
+                        }
+                      ]
+                    }
+                  ]
+                }
+              ]
+            ]
+          },
+          {
+            "accno": "source_chars-E-MTAB-6879",
+            "type": "Source Characteristics",
+            "attributes": [
+              {
+                "name": "Organism",
+                "value": "Mus musculus"
+              },
+              {
+                "name": "Strain",
+                "value": "C57BL/6"
+              },
+              {
+                "name": "Sex",
+                "value": "female"
+              },
+              {
+                "name": "Organism part",
+                "value": "stomach"
+              },
+              {
+                "name": "Cell type",
+                "value": "epithelial cell"
+              },
+              {
+                "name": "Genotype",
+                "value": "Pgc-IRES-DTR-T2A-dsRed"
+              },
+              {
+                "name": "Genotype",
+                "value": "Stmn1-P2A-eGFP-IRES-CreERT2"
+              },
+              {
+                "name": "Individual",
+                "value": "21197"
+              },
+              {
+                "name": "Individual",
+                "value": "23003"
+              },
+              {
+                "name": "Individual",
+                "value": "21267"
+              },
+              {
+                "name": "Individual",
+                "value": "21196"
+              },
+              {
+                "name": "Individual",
+                "value": "23002"
+              },
+              {
+                "name": "Age",
+                "value": "9 week"
+              },
+              {
+                "name": "Age",
+                "value": "9.9 week"
+              },
+              {
+                "name": "Phenotype",
+                "value": "Stmn1 positive"
+              },
+              {
+                "name": "Phenotype",
+                "value": "Pgc positive"
+              },
+              {
+                "name": "Single cell well quality",
+                "value": "OK"
+              },
+              {
+                "name": "Single cell well quality",
+                "value": "not OK"
+              },
+              {
+                "name": "Post analysis well quality",
+                "value": "fail"
+              },
+              {
+                "name": "Post analysis well quality",
+                "value": "pass"
+              }
+            ],
+            "subsections": [
+              [
+                {
+                  "accno": "source_0",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21196"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "130",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546680,ERS2546682,ERS2546690,ERS2546692,ERS2546699,ERS2546705,ERS2546706,ERS2546711,ERS2546713,ERS2546714,ERS2546716,ERS2546720,ERS2546722,ERS2546724,ERS2546726,ERS2546727,ERS2546729,ERS2546730,ERS2546731,ERS2546735,ERS2546742,ERS2546743,ERS2546744,ERS2546751,ERS2546752,ERS2546756,ERS2546758,ERS2546763,ERS2546767,ERS2546768,ERS2546770,ERS2546775,ERS2546781,ERS2546785,ERS2546786,ERS2546787,ERS2546789,ERS2546792,ERS2546795,ERS2546800,ERS2546802,ERS2546803,ERS2546809,ERS2546810,ERS2546811,ERS2546812,ERS2546816,ERS2546818,ERS2546819,ERS2546820,ERS2546821,ERS2546823,ERS2546824,ERS2546826,ERS2546828,ERS2547604,ERS2547607,ERS2547610,ERS2547615,ERS2547616,ERS2547617,ERS2547618,ERS2547620,ERS2547621,ERS2547622,ERS2547623,ERS2547624,ERS2547626,ERS2547627,ERS2547629,ERS2547630,ERS2547632,ERS2547637,ERS2547641,ERS2547642,ERS2547643,ERS2547645,ERS2547651,ERS2547655,ERS2547658,ERS2547660,ERS2547662,ERS2547667,ERS2547669,ERS2547676,ERS2547684,ERS2547690,ERS2547693,ERS2547702,ERS2547705,ERS2547714,ERS2547715,ERS2547717,ERS2547726,ERS2547727,ERS2547730,ERS2547731,ERS2547732,ERS2547736,ERS2547737,ERS2547741,ERS2547744,ERS2547748,ERS2547750,ERS2547752,ERS2547753,ERS2547755,ERS2547756,ERS2547759,ERS2547760,ERS2547761,ERS2547764,ERS2547765,ERS2547766,ERS2547768,ERS2547769,ERS2547770,ERS2547771,ERS2547783,ERS2547787,ERS2547790,ERS2547792,ERS2547796,ERS2547797,ERS2547801,ERS2547811,ERS2547816,ERS2547827,ERS2547830,ERS2547831"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_1",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21196"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "pass"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "249",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546681,ERS2546683,ERS2546684,ERS2546685,ERS2546686,ERS2546687,ERS2546688,ERS2546689,ERS2546691,ERS2546693,ERS2546694,ERS2546695,ERS2546696,ERS2546697,ERS2546698,ERS2546700,ERS2546701,ERS2546702,ERS2546703,ERS2546704,ERS2546707,ERS2546708,ERS2546709,ERS2546710,ERS2546712,ERS2546715,ERS2546717,ERS2546718,ERS2546719,ERS2546721,ERS2546723,ERS2546725,ERS2546728,ERS2546732,ERS2546733,ERS2546734,ERS2546736,ERS2546737,ERS2546738,ERS2546739,ERS2546740,ERS2546741,ERS2546745,ERS2546746,ERS2546747,ERS2546748,ERS2546749,ERS2546750,ERS2546753,ERS2546754,ERS2546755,ERS2546757,ERS2546759,ERS2546760,ERS2546761,ERS2546762,ERS2546764,ERS2546765,ERS2546766,ERS2546769,ERS2546771,ERS2546772,ERS2546773,ERS2546774,ERS2546776,ERS2546777,ERS2546778,ERS2546779,ERS2546780,ERS2546782,ERS2546783,ERS2546784,ERS2546788,ERS2546790,ERS2546791,ERS2546793,ERS2546794,ERS2546796,ERS2546797,ERS2546798,ERS2546799,ERS2546801,ERS2546804,ERS2546805,ERS2546806,ERS2546807,ERS2546808,ERS2546813,ERS2546814,ERS2546815,ERS2546817,ERS2546822,ERS2546825,ERS2546827,ERS2547602,ERS2547603,ERS2547605,ERS2547606,ERS2547608,ERS2547609,ERS2547611,ERS2547612,ERS2547613,ERS2547614,ERS2547619,ERS2547625,ERS2547628,ERS2547631,ERS2547633,ERS2547634,ERS2547635,ERS2547636,ERS2547638,ERS2547639,ERS2547640,ERS2547644,ERS2547646,ERS2547647,ERS2547648,ERS2547649,ERS2547650,ERS2547652,ERS2547653,ERS2547654,ERS2547656,ERS2547657,ERS2547659,ERS2547661,ERS2547663,ERS2547664,ERS2547665,ERS2547666,ERS2547668,ERS2547670,ERS2547671,ERS2547672,ERS2547673,ERS2547674,ERS2547675,ERS2547677,ERS2547678,ERS2547679,ERS2547680,ERS2547681,ERS2547682,ERS2547683,ERS2547685,ERS2547686,ERS2547687,ERS2547688,ERS2547689,ERS2547691,ERS2547692,ERS2547694,ERS2547695,ERS2547696,ERS2547697,ERS2547698,ERS2547699,ERS2547700,ERS2547701,ERS2547703,ERS2547704,ERS2547706,ERS2547707,ERS2547708,ERS2547709,ERS2547710,ERS2547711,ERS2547712,ERS2547713,ERS2547716,ERS2547718,ERS2547719,ERS2547720,ERS2547721,ERS2547722,ERS2547723,ERS2547724,ERS2547725,ERS2547728,ERS2547729,ERS2547733,ERS2547734,ERS2547735,ERS2547738,ERS2547739,ERS2547740,ERS2547742,ERS2547743,ERS2547745,ERS2547746,ERS2547747,ERS2547749,ERS2547751,ERS2547754,ERS2547757,ERS2547758,ERS2547762,ERS2547763,ERS2547767,ERS2547772,ERS2547773,ERS2547774,ERS2547775,ERS2547776,ERS2547777,ERS2547778,ERS2547779,ERS2547780,ERS2547781,ERS2547782,ERS2547784,ERS2547785,ERS2547786,ERS2547788,ERS2547789,ERS2547791,ERS2547793,ERS2547794,ERS2547795,ERS2547798,ERS2547799,ERS2547800,ERS2547802,ERS2547803,ERS2547804,ERS2547805,ERS2547806,ERS2547807,ERS2547808,ERS2547809,ERS2547810,ERS2547812,ERS2547813,ERS2547814,ERS2547815,ERS2547817,ERS2547818,ERS2547819,ERS2547820,ERS2547821,ERS2547822,ERS2547823,ERS2547824,ERS2547825,ERS2547826,ERS2547828,ERS2547829"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_2",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21196"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "not OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "5",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546829,ERS2546830,ERS2546831,ERS2546832,ERS2547601"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_3",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21197"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "not OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "5",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546833,ERS2547213,ERS2547214,ERS2547215,ERS2547216"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_4",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21197"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "105",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546834,ERS2546837,ERS2546838,ERS2546841,ERS2546843,ERS2546844,ERS2546845,ERS2546847,ERS2546850,ERS2546851,ERS2546852,ERS2546855,ERS2546858,ERS2546863,ERS2546864,ERS2546873,ERS2546880,ERS2546884,ERS2546888,ERS2546899,ERS2546900,ERS2546902,ERS2546903,ERS2546904,ERS2546906,ERS2546907,ERS2546917,ERS2546919,ERS2546922,ERS2546926,ERS2546931,ERS2546932,ERS2546935,ERS2546937,ERS2546948,ERS2546953,ERS2546954,ERS2546962,ERS2546971,ERS2546972,ERS2546977,ERS2546980,ERS2546984,ERS2546985,ERS2546986,ERS2546988,ERS2546993,ERS2546995,ERS2547003,ERS2547004,ERS2547011,ERS2547014,ERS2547016,ERS2547017,ERS2547018,ERS2547021,ERS2547026,ERS2547029,ERS2547033,ERS2547043,ERS2547045,ERS2547048,ERS2547049,ERS2547052,ERS2547057,ERS2547058,ERS2547059,ERS2547061,ERS2547064,ERS2547065,ERS2547068,ERS2547069,ERS2547073,ERS2547076,ERS2547080,ERS2547081,ERS2547087,ERS2547088,ERS2547089,ERS2547094,ERS2547099,ERS2547104,ERS2547105,ERS2547110,ERS2547113,ERS2547116,ERS2547117,ERS2547118,ERS2547128,ERS2547138,ERS2547149,ERS2547156,ERS2547157,ERS2547162,ERS2547164,ERS2547168,ERS2547169,ERS2547171,ERS2547172,ERS2547178,ERS2547187,ERS2547194,ERS2547196,ERS2547201,ERS2547204"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_5",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21197"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "pass"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "274",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2546835,ERS2546836,ERS2546839,ERS2546840,ERS2546842,ERS2546846,ERS2546848,ERS2546849,ERS2546853,ERS2546854,ERS2546856,ERS2546857,ERS2546859,ERS2546860,ERS2546861,ERS2546862,ERS2546865,ERS2546866,ERS2546867,ERS2546868,ERS2546869,ERS2546870,ERS2546871,ERS2546872,ERS2546874,ERS2546875,ERS2546876,ERS2546877,ERS2546878,ERS2546879,ERS2546881,ERS2546882,ERS2546883,ERS2546885,ERS2546886,ERS2546887,ERS2546889,ERS2546890,ERS2546891,ERS2546892,ERS2546893,ERS2546894,ERS2546895,ERS2546896,ERS2546897,ERS2546898,ERS2546901,ERS2546905,ERS2546908,ERS2546909,ERS2546910,ERS2546911,ERS2546912,ERS2546913,ERS2546914,ERS2546915,ERS2546916,ERS2546918,ERS2546920,ERS2546921,ERS2546923,ERS2546924,ERS2546925,ERS2546927,ERS2546928,ERS2546929,ERS2546930,ERS2546933,ERS2546934,ERS2546936,ERS2546938,ERS2546939,ERS2546940,ERS2546941,ERS2546942,ERS2546943,ERS2546944,ERS2546945,ERS2546946,ERS2546947,ERS2546949,ERS2546950,ERS2546951,ERS2546952,ERS2546955,ERS2546956,ERS2546957,ERS2546958,ERS2546959,ERS2546960,ERS2546961,ERS2546963,ERS2546964,ERS2546965,ERS2546966,ERS2546967,ERS2546968,ERS2546969,ERS2546970,ERS2546973,ERS2546974,ERS2546975,ERS2546976,ERS2546978,ERS2546979,ERS2546981,ERS2546982,ERS2546983,ERS2546987,ERS2546989,ERS2546990,ERS2546991,ERS2546992,ERS2546994,ERS2546996,ERS2546997,ERS2546998,ERS2546999,ERS2547000,ERS2547001,ERS2547002,ERS2547005,ERS2547006,ERS2547007,ERS2547008,ERS2547009,ERS2547010,ERS2547012,ERS2547013,ERS2547015,ERS2547019,ERS2547020,ERS2547022,ERS2547023,ERS2547024,ERS2547025,ERS2547027,ERS2547028,ERS2547030,ERS2547031,ERS2547032,ERS2547034,ERS2547035,ERS2547036,ERS2547037,ERS2547038,ERS2547039,ERS2547040,ERS2547041,ERS2547042,ERS2547044,ERS2547046,ERS2547047,ERS2547050,ERS2547051,ERS2547053,ERS2547054,ERS2547055,ERS2547056,ERS2547060,ERS2547062,ERS2547063,ERS2547066,ERS2547067,ERS2547070,ERS2547071,ERS2547072,ERS2547074,ERS2547075,ERS2547077,ERS2547078,ERS2547079,ERS2547082,ERS2547083,ERS2547084,ERS2547085,ERS2547086,ERS2547090,ERS2547091,ERS2547092,ERS2547093,ERS2547095,ERS2547096,ERS2547097,ERS2547098,ERS2547100,ERS2547101,ERS2547102,ERS2547103,ERS2547106,ERS2547107,ERS2547108,ERS2547109,ERS2547111,ERS2547112,ERS2547114,ERS2547115,ERS2547119,ERS2547120,ERS2547121,ERS2547122,ERS2547123,ERS2547124,ERS2547125,ERS2547126,ERS2547127,ERS2547129,ERS2547130,ERS2547131,ERS2547132,ERS2547133,ERS2547134,ERS2547135,ERS2547136,ERS2547137,ERS2547139,ERS2547140,ERS2547141,ERS2547142,ERS2547143,ERS2547144,ERS2547145,ERS2547146,ERS2547147,ERS2547148,ERS2547150,ERS2547151,ERS2547152,ERS2547153,ERS2547154,ERS2547155,ERS2547158,ERS2547159,ERS2547160,ERS2547161,ERS2547163,ERS2547165,ERS2547166,ERS2547167,ERS2547170,ERS2547173,ERS2547174,ERS2547175,ERS2547176,ERS2547177,ERS2547179,ERS2547180,ERS2547181,ERS2547182,ERS2547183,ERS2547184,ERS2547185,ERS2547186,ERS2547188,ERS2547189,ERS2547190,ERS2547191,ERS2547192,ERS2547193,ERS2547195,ERS2547197,ERS2547198,ERS2547199,ERS2547200,ERS2547202,ERS2547203,ERS2547205,ERS2547206,ERS2547207,ERS2547208,ERS2547209,ERS2547210,ERS2547211,ERS2547212"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_6",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21267"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "not OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "5",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547217,ERS2547597,ERS2547598,ERS2547599,ERS2547600"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_7",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21267"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "pass"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "220",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547218,ERS2547219,ERS2547220,ERS2547221,ERS2547222,ERS2547223,ERS2547224,ERS2547225,ERS2547227,ERS2547228,ERS2547229,ERS2547230,ERS2547232,ERS2547235,ERS2547238,ERS2547239,ERS2547240,ERS2547241,ERS2547242,ERS2547245,ERS2547246,ERS2547249,ERS2547251,ERS2547252,ERS2547254,ERS2547256,ERS2547258,ERS2547259,ERS2547263,ERS2547265,ERS2547269,ERS2547272,ERS2547276,ERS2547277,ERS2547279,ERS2547281,ERS2547282,ERS2547283,ERS2547284,ERS2547286,ERS2547290,ERS2547293,ERS2547296,ERS2547298,ERS2547299,ERS2547300,ERS2547301,ERS2547302,ERS2547307,ERS2547309,ERS2547310,ERS2547314,ERS2547315,ERS2547316,ERS2547317,ERS2547318,ERS2547322,ERS2547325,ERS2547326,ERS2547327,ERS2547329,ERS2547330,ERS2547331,ERS2547333,ERS2547334,ERS2547335,ERS2547340,ERS2547341,ERS2547342,ERS2547343,ERS2547344,ERS2547345,ERS2547347,ERS2547348,ERS2547349,ERS2547350,ERS2547353,ERS2547355,ERS2547356,ERS2547357,ERS2547362,ERS2547366,ERS2547367,ERS2547370,ERS2547371,ERS2547375,ERS2547378,ERS2547380,ERS2547381,ERS2547382,ERS2547383,ERS2547386,ERS2547387,ERS2547388,ERS2547389,ERS2547391,ERS2547394,ERS2547395,ERS2547396,ERS2547397,ERS2547403,ERS2547405,ERS2547406,ERS2547408,ERS2547409,ERS2547410,ERS2547411,ERS2547412,ERS2547413,ERS2547414,ERS2547415,ERS2547417,ERS2547418,ERS2547419,ERS2547420,ERS2547421,ERS2547423,ERS2547425,ERS2547426,ERS2547429,ERS2547430,ERS2547431,ERS2547432,ERS2547433,ERS2547435,ERS2547436,ERS2547439,ERS2547440,ERS2547442,ERS2547443,ERS2547445,ERS2547446,ERS2547447,ERS2547448,ERS2547449,ERS2547450,ERS2547451,ERS2547452,ERS2547454,ERS2547455,ERS2547456,ERS2547457,ERS2547459,ERS2547460,ERS2547464,ERS2547466,ERS2547467,ERS2547470,ERS2547471,ERS2547475,ERS2547476,ERS2547477,ERS2547478,ERS2547480,ERS2547481,ERS2547484,ERS2547485,ERS2547486,ERS2547488,ERS2547490,ERS2547491,ERS2547492,ERS2547493,ERS2547496,ERS2547497,ERS2547499,ERS2547500,ERS2547502,ERS2547504,ERS2547505,ERS2547506,ERS2547510,ERS2547512,ERS2547513,ERS2547514,ERS2547515,ERS2547516,ERS2547517,ERS2547518,ERS2547520,ERS2547524,ERS2547525,ERS2547529,ERS2547531,ERS2547533,ERS2547534,ERS2547536,ERS2547537,ERS2547538,ERS2547539,ERS2547540,ERS2547541,ERS2547542,ERS2547545,ERS2547547,ERS2547549,ERS2547555,ERS2547557,ERS2547558,ERS2547559,ERS2547561,ERS2547567,ERS2547570,ERS2547572,ERS2547573,ERS2547576,ERS2547579,ERS2547580,ERS2547581,ERS2547583,ERS2547584,ERS2547587,ERS2547588,ERS2547589,ERS2547590,ERS2547591,ERS2547593,ERS2547594,ERS2547595,ERS2547596"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_8",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Pgc-IRES-DTR-T2A-dsRed"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "21267"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Pgc positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "159",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547226,ERS2547231,ERS2547233,ERS2547234,ERS2547236,ERS2547237,ERS2547243,ERS2547244,ERS2547247,ERS2547248,ERS2547250,ERS2547253,ERS2547255,ERS2547257,ERS2547260,ERS2547261,ERS2547262,ERS2547264,ERS2547266,ERS2547267,ERS2547268,ERS2547270,ERS2547271,ERS2547273,ERS2547274,ERS2547275,ERS2547278,ERS2547280,ERS2547285,ERS2547287,ERS2547288,ERS2547289,ERS2547291,ERS2547292,ERS2547294,ERS2547295,ERS2547297,ERS2547303,ERS2547304,ERS2547305,ERS2547306,ERS2547308,ERS2547311,ERS2547312,ERS2547313,ERS2547319,ERS2547320,ERS2547321,ERS2547323,ERS2547324,ERS2547328,ERS2547332,ERS2547336,ERS2547337,ERS2547338,ERS2547339,ERS2547346,ERS2547351,ERS2547352,ERS2547354,ERS2547358,ERS2547359,ERS2547360,ERS2547361,ERS2547363,ERS2547364,ERS2547365,ERS2547368,ERS2547369,ERS2547372,ERS2547373,ERS2547374,ERS2547376,ERS2547377,ERS2547379,ERS2547384,ERS2547385,ERS2547390,ERS2547392,ERS2547393,ERS2547398,ERS2547399,ERS2547400,ERS2547401,ERS2547402,ERS2547404,ERS2547407,ERS2547416,ERS2547422,ERS2547424,ERS2547427,ERS2547428,ERS2547434,ERS2547437,ERS2547438,ERS2547441,ERS2547444,ERS2547453,ERS2547458,ERS2547461,ERS2547462,ERS2547463,ERS2547465,ERS2547468,ERS2547469,ERS2547472,ERS2547473,ERS2547474,ERS2547479,ERS2547482,ERS2547483,ERS2547487,ERS2547489,ERS2547494,ERS2547495,ERS2547498,ERS2547501,ERS2547503,ERS2547507,ERS2547508,ERS2547509,ERS2547511,ERS2547519,ERS2547521,ERS2547522,ERS2547523,ERS2547526,ERS2547527,ERS2547528,ERS2547530,ERS2547532,ERS2547535,ERS2547543,ERS2547544,ERS2547546,ERS2547548,ERS2547550,ERS2547551,ERS2547552,ERS2547553,ERS2547554,ERS2547556,ERS2547560,ERS2547562,ERS2547563,ERS2547564,ERS2547565,ERS2547566,ERS2547568,ERS2547569,ERS2547571,ERS2547574,ERS2547575,ERS2547577,ERS2547578,ERS2547582,ERS2547585,ERS2547586,ERS2547592"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_9",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Stmn1-P2A-eGFP-IRES-CreERT2"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "23002"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9.9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Stmn1 positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "not OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "5",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547832,ERS2548202,ERS2548203,ERS2548204,ERS2548205"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_10",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Stmn1-P2A-eGFP-IRES-CreERT2"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "23002"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9.9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Stmn1 positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "72",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2547833,ERS2547835,ERS2547837,ERS2547839,ERS2547844,ERS2547851,ERS2547852,ERS2547855,ERS2547858,ERS2547863,ERS2547867,ERS2547872,ERS2547877,ERS2547892,ERS2547903,ERS2547912,ERS2547918,ERS2547924,ERS2547936,ERS2547944,ERS2547949,ERS2547952,ERS2547966,ERS2547968,ERS2547975,ERS2547976,ERS2547980,ERS2547988,ERS2547989,ERS2547991,ERS2548002,ERS2548008,ERS2548012,ERS2548016,ERS2548020,ERS2548023,ERS2548033,ERS2548052,ERS2548059,ERS2548101,ERS2548104,ERS2548108,ERS2548125,ERS2548137,ERS2548146,ERS2548152,ERS2548162,ERS2548163,ERS2548169,ERS2548176,ERS2548179,ERS2548180,ERS2548181,ERS2548182,ERS2548183,ERS2548184,ERS2548187,ERS2548191,ERS2548195,ERS2548200,ERS2548234,ERS2548256,ERS2548289,ERS2548311,ERS2548345,ERS2548367,ERS2548544,ERS2548641,ERS2548646,ERS2548650,ERS2548651,ERS2548652"
+                        }
+                      ]
+                    }
+                  ]
+                },
+                {
+                  "accno": "source_11",
+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Stmn1-P2A-eGFP-IRES-CreERT2"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "23002"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9.9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Stmn1 positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "pass"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "307",
+                      "valqual": [
+                        {
+                          "name": "url",
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+                  "type": "Characteristics Table",
+                  "attributes": [
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+                      "name": "genotype",
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+                      "value": "23003"
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+                      "value": "9.9 week"
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+                    {
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+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "OK"
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+                    {
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+                      "value": "fail"
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+                    {
+                      "name": "No. of Samples",
+                      "value": "76",
+                      "valqual": [
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+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2548215,ERS2548218,ERS2548219,ERS2548220,ERS2548221,ERS2548224,ERS2548225,ERS2548226,ERS2548227,ERS2548228,ERS2548229,ERS2548230,ERS2548231,ERS2548232,ERS2548241,ERS2548242,ERS2548243,ERS2548248,ERS2548249,ERS2548251,ERS2548252,ERS2548253,ERS2548254,ERS2548269,ERS2548270,ERS2548271,ERS2548275,ERS2548279,ERS2548284,ERS2548297,ERS2548298,ERS2548306,ERS2548313,ERS2548314,ERS2548344,ERS2548346,ERS2548359,ERS2548365,ERS2548369,ERS2548374,ERS2548377,ERS2548381,ERS2548382,ERS2548386,ERS2548391,ERS2548393,ERS2548395,ERS2548408,ERS2548410,ERS2548415,ERS2548431,ERS2548453,ERS2548463,ERS2548485,ERS2548487,ERS2548502,ERS2548505,ERS2548508,ERS2548520,ERS2548523,ERS2548531,ERS2548536,ERS2548537,ERS2548541,ERS2548548,ERS2548561,ERS2548569,ERS2548576,ERS2548580,ERS2548605,ERS2548606,ERS2548607,ERS2548610,ERS2548617,ERS2548624,ERS2548627"
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+                  "type": "Characteristics Table",
+                  "attributes": [
+                    {
+                      "name": "genotype",
+                      "value": "Stmn1-P2A-eGFP-IRES-CreERT2"
+                    },
+                    {
+                      "name": "individual",
+                      "value": "23003"
+                    },
+                    {
+                      "name": "age",
+                      "value": "9.9 week"
+                    },
+                    {
+                      "name": "phenotype",
+                      "value": "Stmn1 positive"
+                    },
+                    {
+                      "name": "single cell well quality",
+                      "value": "not OK"
+                    },
+                    {
+                      "name": "post analysis well quality",
+                      "value": "fail"
+                    },
+                    {
+                      "name": "No. of Samples",
+                      "value": "5",
+                      "valqual": [
+                        {
+                          "name": "url",
+                          "value": "https://www.ebi.ac.uk/ena/browser/view/ERS2548217,ERS2548628,ERS2548629,ERS2548630,ERS2548631"
+                        }
+                      ]
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+                  ]
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+        ]
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+      {
+        "accno": "s-assays-data-E-MTAB-6879",
+        "type": "Assays and Data",
+        "attributes": [
+          {
+            "name": "Assay count",
+            "value": "1920"
+          },
+          {
+            "name": "Technology",
+            "value": "Sequencing assay"
+          },
+          {
+            "name": "Assay by Molecule",
+            "value": "RNA assay"
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+          {
+            "accno": "processed-data",
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+              [
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+                      "value": "Processed Data"
+                    }
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+                  "type": "file"
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+              ]
+            ]
+          },
+          {
+            "accno": "mt-E-MTAB-6879",
+            "type": "MAGE-TAB Files",
+            "files": [
+              [
+                {
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+                  "size": 7997,
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+                    {
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+                      "name": "Description",
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+                    {
+                      "name": "Description",
+                      "value": "Sample and Data Relationship Format (SDRF)"
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+      {
+        "accno": "score-E-MTAB-6879",
+        "type": "MINSEQE Score",
+        "attributes": [
+          {
+            "name": "Exp. Design",
+            "value": "*"
+          },
+          {
+            "name": "Protocols",
+            "value": "*"
+          },
+          {
+            "name": "Variables",
+            "value": "*"
+          },
+          {
+            "name": "Raw",
+            "value": "*"
+          },
+          {
+            "name": "Processed",
+            "value": "*"
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+        ]
+      }
+    ]
+  },
+  "type": "submission"
+}

脓毒症/单细胞组/arrayexpress_E-MTAB-6879/card.md

@@ -0,0 +1,37 @@
+# E-MTAB-6879 — Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level
+
+> 脓毒症(Sepsis)相关数据集 · 信息卡 · 由自动采集流水线生成 2026-06-21 07:04:44 UTC
+
+## 基本信息
+
+| 字段 | 内容 |
+|---|---|
+| 登记号 Accession | `E-MTAB-6879` |
+| 数据源 Source | ArrayExpress/BioStudies |
+| 数据类型 Type | 单细胞组 (single_cell) |
+| 物种 Organism | — |
+| 样本数 Samples/Cells | — |
+| 年份 Year | 2019 |
+| 原始页面 Landing | https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-6879 |
+| 下载层级 Level | 处理数据+元数据 |
+| 本地下载大小 | 189.04 MB |
+
+## 摘要 Summary
+
+Seungmin Han Bon-Kyoung Koo
+
+## 文件 Files
+
+| 文件 | 大小(MB) | 链接/备注 |
+|---|---|---|
+| `biostudies.json` | metadata | (metadata) |
+| `all_samples_normalized_readcounts.txt` | 186.310 | https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/all_samples_normalized_readcounts.txt |
+| `E-MTAB-6879.idf.txt` | 0.008 | https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/E-MTAB-6879.idf.txt |
+| `E-MTAB-6879.sdrf.txt` | 2.721 | https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/E-MTAB-6879.sdrf.txt |
+
+## 后续分析建议 Analysis hints
+
+Scanpy/Seurat 读取 h5ad/matrix → QC → 整合 → 聚类注释 → 差异/通路。
+
+---
+*脓毒症数据采集流水线 · Hugging Face 镜像 · 数据版权归原始提交者，使用请遵循各库许可与引用要求。*

脓毒症/单细胞组/arrayexpress_E-MTAB-6879/metadata.json

@@ -0,0 +1,36 @@
+{
+  "source": "arrayexpress",
+  "accession": "E-MTAB-6879",
+  "data_type": "single_cell",
+  "title": "Single cell RNA-seq of Stmn1+ isthmal cells and Pgc+ chief cells to compare and characterize distinct gastric corpus stem cells at molecular level",
+  "organism": "",
+  "n_samples": "",
+  "year": "2019",
+  "summary": "Seungmin Han Bon-Kyoung Koo",
+  "landing_url": "https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-6879",
+  "files": [
+    {
+      "name": "biostudies.json",
+      "url": "(metadata)",
+      "size_mb": 0
+    },
+    {
+      "name": "all_samples_normalized_readcounts.txt",
+      "url": "https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/all_samples_normalized_readcounts.txt",
+      "size_mb": 186.31
+    },
+    {
+      "name": "E-MTAB-6879.idf.txt",
+      "url": "https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/E-MTAB-6879.idf.txt",
+      "size_mb": 0.008
+    },
+    {
+      "name": "E-MTAB-6879.sdrf.txt",
+      "url": "https://www.ebi.ac.uk/biostudies/files/E-MTAB-6879/E-MTAB-6879.sdrf.txt",
+      "size_mb": 2.721
+    }
+  ],
+  "level": "处理数据+元数据",
+  "harvested_utc": "2026-06-21 07:04:44",
+  "focus": true
+}