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README.md
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## **Fine-Tuning ESM-1b with Multiple Sequence Alignment (MSA) for Phosphosites**
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This repository provides a fine-tuned version of ESM-1b, incorporating genomic information by leveraging long phosphosite sequences from [DARKIN dataset](https://openreview.net/pdf?id=a4x5tbYRYV) and Multiple Sequence Alignment (MSA) of those phosphosites. The goal is to enhance the model's understanding of phosphorylation by integrating sequence conservation patterns.
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### Developed by:
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- 10% of the data was reserved for validation.
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- The remaining 90% was used for fine-tuning with the Masked Language Modeling (MLM) objective.
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3. Data Processing & Preprocessing
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- Special attention was given to
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- To optimize memory efficiency, sequence lengths were truncated to 128 amino acids.
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### Evaluation
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Perplexity: 2.69 (decreased from 7.05)
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from transformers import AutoTokenizer, AutoModelForMaskedLM
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import torch
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### Usage
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```
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# Load the model and tokenizer
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model_name = "isikz/phosphosite_msa_finetuned_esm1b"
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tokenizer = AutoTokenizer.from_pretrained(model_name)
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## **Fine-Tuning ESM-1b with Multiple Sequence Alignment (MSA) for Phosphosites**
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+
This repository provides a fine-tuned version of ESM-1b with Masked Language Modeling(MLM) Objective, incorporating genomic information by leveraging long phosphosite sequences from [DARKIN dataset](https://openreview.net/pdf?id=a4x5tbYRYV) and Multiple Sequence Alignment (MSA) of those phosphosites. The goal is to enhance the model's understanding of phosphorylation by integrating sequence conservation patterns.
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### Developed by:
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- 10% of the data was reserved for validation.
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- The remaining 90% was used for fine-tuning with the Masked Language Modeling (MLM) objective.
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3. Data Processing & Preprocessing
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- Special attention was given to conserving phosphorylation residues within sequences.
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- To optimize memory efficiency, sequence lengths were truncated to 128 amino acids.
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### Evaluation
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Perplexity: 2.69 (decreased from 7.05)
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### Usage
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```
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from transformers import AutoTokenizer, AutoModelForMaskedLM
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import torch
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# Load the model and tokenizer
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model_name = "isikz/phosphosite_msa_finetuned_esm1b"
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tokenizer = AutoTokenizer.from_pretrained(model_name)
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