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import gradio as gr
from queue import Queue
import threading
import time
import paho.mqtt.client as mqtt
import json
import secrets
from DB_utils import find_unused_wells, update_used_wells, save_result, get_student_quota, decrement_student_quota
import os
from yt_utils import get_latest_video_id
from prefect import flow
from prefect_utils import start_prefect_worker
# NOTE: New global dict to store tasks keyed by (student_id, experiment_id)
tasks_dict = {}
task_queue = Queue()
result_queue = Queue()
current_task = None
sensor_results = None
queue_counter = task_queue.qsize()
MQTT_BROKER = os.getenv("MQTT_BROKER")
MQTT_PORT = int(os.getenv("MQTT_PORT"))
MQTT_USERNAME = os.getenv("MQTT_USERNAME")
MQTT_PASSWORD = os.getenv("MQTT_PASSWORD")
OT2_SERIAL = "OT2CEP20240218R04"
PICO_ID = "e66130100f895134"
OT2_COMMAND_TOPIC = f"command/ot2/{OT2_SERIAL}/pipette"
OT2_STATUS_TOPIC = f"status/ot2/{OT2_SERIAL}/complete"
SENSOR_COMMAND_TOPIC = f"command/picow/{PICO_ID}/as7341/read"
SENSOR_DATA_TOPIC = f"color-mixing/picow/{PICO_ID}/as7341"
channel_id = "UCHBzCfYpGwoqygH9YNh9A6g"
# Single source of truth for the playlist you care about
PLAYLIST_ID = "PL8uZlc2CEpelFebA8UgFMQP4Y1d5X_pME"
# Optional: initial fetch/log (not strictly required by Gradio app)
video_id = get_latest_video_id(channel_id, playlist_id=PLAYLIST_ID)
print(f"[DEBUG] initial video id: {video_id}")
start_prefect_worker()
print("[TEST]Prefect worker initialized")
def check_student_quota(student_id):
"""Check student's remaining experiment quota"""
student_quota = get_student_quota(student_id)
return student_quota
def validate_ryb_input(R, Y, B):
"""Validate RYB input volumes"""
total = R + Y + B
if total > 300:
return {
"is_valid": False,
"message": f"Total volume cannot exceed 300 µL. Current total: {total} µL."
}
return {
"is_valid": True,
"message": f"Current total: {total} µL."
}
mqtt_client = mqtt.Client()
mqtt_client.tls_set(tls_version=mqtt.ssl.PROTOCOL_TLS_CLIENT)
mqtt_client.username_pw_set(MQTT_USERNAME, MQTT_PASSWORD)
def on_connect(client, userdata, flags, rc):
print(f"Connected to MQTT Broker with result code {rc}")
client.subscribe([(OT2_STATUS_TOPIC, 2), (SENSOR_DATA_TOPIC, 2)])
def on_message(client, userdata, msg):
global current_task, sensor_results
try:
payload = json.loads(msg.payload.decode("utf-8"))
if msg.topic == OT2_STATUS_TOPIC:
handle_sensor_status(payload)
elif msg.topic == SENSOR_DATA_TOPIC:
print("Sensor data received")
sensor_results = payload
mqtt_client.publish(
OT2_COMMAND_TOPIC,
json.dumps({
"command": {"sensor_status": "read"},
"experiment_id": payload["experiment_id"],
"session_id": payload["session_id"]
}),
)
except Exception as e:
print(f"Error processing MQTT message: {e}")
mqtt_client.on_connect = on_connect
mqtt_client.on_message = on_message
mqtt_client.connect(MQTT_BROKER, MQTT_PORT)
mqtt_client.loop_start()
def handle_sensor_status(payload):
global current_task, sensor_results
if "in_place" in json.dumps(payload):
mqtt_client.publish(
SENSOR_COMMAND_TOPIC,
json.dumps({
"command": {
"R": current_task["R"],
"Y": current_task["Y"],
"B": current_task["B"],
"well": current_task["well"]
},
"experiment_id": current_task["experiment_id"],
"session_id": current_task["session_id"]
})
)
elif payload["status"]["sensor_status"] == "charging":
experiment_result = {
"Status": "Complete",
"Message": "Experiment completed successfully!",
"Student ID": current_task["session_id"],
"Command": {
"R": current_task["R"],
"Y": current_task["Y"],
"B": current_task["B"],
"well": current_task["well"],
},
"Sensor Data": sensor_results["sensor_data"],
"Experiment ID": current_task["experiment_id"]
}
# Store full result in result queue
result_queue.put(experiment_result)
# Create a version of experiment_result without "Status" and "Message" for database storage
db_data = {key: experiment_result[key] for key in experiment_result if key not in ["Status", "Message"]}
save_result(db_data)
current_task = None
def task_processor():
"""
Background thread that processes tasks one by one.
"""
global current_task, queue_counter
task_start_time = None
TIMEOUT_SECONDS = 165
while True:
if current_task:
# Check for timeout
if task_start_time and (time.time() - task_start_time > TIMEOUT_SECONDS):
print("sending timeout message to OT-2")
mqtt_client.publish(
OT2_COMMAND_TOPIC,
json.dumps({
"command": {"sensor_status": "sensor_timeout"},
"experiment_id": current_task["experiment_id"],
"session_id": current_task["session_id"]
}),
)
result_queue.put({
"Status": "Error",
"Message": "Experiment timed out",
"Student ID": current_task["session_id"],
"Command": {
"R": current_task["R"],
"Y": current_task["Y"],
"B": current_task["B"],
"well": current_task["well"],
},
"Experiment ID": current_task["experiment_id"]
})
current_task = None
task_start_time = None
continue
if not current_task and not task_queue.empty():
# Fetch a new task from the queue
student_id, experiment_id = task_queue.get() # NOTE: We'll store (student_id, experiment_id) instead of task
queue_counter -= 1
task_start_time = time.time()
# NOTE: We retrieve the actual task from tasks_dict
current_task = tasks_dict[(student_id, experiment_id)]
# Mark status as "processing"
current_task["status"] = "processing"
mqtt_client.publish(
OT2_COMMAND_TOPIC,
json.dumps({
"command": {
"R": current_task["R"],
"Y": current_task["Y"],
"B": current_task["B"],
"well": current_task["well"]
},
"experiment_id": current_task["experiment_id"],
"session_id": current_task["session_id"]
}),
)
time.sleep(1)
processor_thread = threading.Thread(target=task_processor, daemon=True)
processor_thread.start()
def verify_student_id(student_id):
"""Verify student ID and check quota"""
global queue_counter
if not student_id:
return [
gr.update(interactive=False, value=0),
gr.update(interactive=False, value=0),
gr.update(interactive=False, value=0),
"Please enter a Student ID",
gr.update(interactive=False)
]
quota_remaining = check_student_quota(student_id)
if quota_remaining <= 0:
return [
gr.update(interactive=False, value=0),
gr.update(interactive=False, value=0),
gr.update(interactive=False, value=0),
"No experiments remaining. Please contact administrator.",
gr.update(interactive=False)
]
return [
gr.update(interactive=True, value=0),
gr.update(interactive=True, value=0),
gr.update(interactive=True, value=0),
f"Student ID verified. Available experiments: {quota_remaining}\nCurrent queue length: {queue_counter} experiment(s)",
gr.update(interactive=True)
]
def update_status_with_queue(R, Y, B):
"""Check if RYB inputs are valid and return updated queue info"""
global queue_counter
validation_result = validate_ryb_input(R, Y, B)
total = R + Y + B
return [
f"{validation_result['message']}\nCurrent queue length: {queue_counter} experiment(s)",
gr.update(interactive=(total <= 300))
]
def update_queue_display():
"""Refresh queue info for the UI"""
global current_task, queue_counter
num_available_wells = len(find_unused_wells())
try:
print(f"[DEBUG] Updating queue display - Counter: {queue_counter}")
if current_task:
status = f"""### Current Queue Status
- Active experiment: Yes
- Queue length: {queue_counter+1} experiment(s)
- Available wells: {num_available_wells} wells
- Expected wait time to obtain results > {(queue_counter+2)*2} mins """
else:
status = f"""### Current Queue Status
- Active experiment: No
- Queue length: {queue_counter} experiment(s)
- Available wells: {num_available_wells} wells
- Expected wait time to obtain results: 2 mins """
return status
except Exception as e:
return f"Error getting queue status: {str(e)}"
def add_to_queue(student_id, R, Y, B):
global queue_counter
if student_id == "debug":
yield {
"Status": "Error",
"Message": "Debug ID cannot submit to real experiment queue. Please use your student id to submit experiment."
}
return
# Validate RYB inputs
validation_result = validate_ryb_input(R, Y, B)
if not validation_result["is_valid"]:
yield {
"Status": "Error",
"Message": validation_result["message"]
}
return
# Check quota
quota_remaining = check_student_quota(student_id)
if quota_remaining <= 0:
yield {
"Status": "Error",
"Message": "No experiments remaining"
}
return
# Select well
experiment_id = secrets.token_hex(4)
try:
empty_wells = find_unused_wells()
if not empty_wells:
raise ValueError("No available wells")
selected_well = empty_wells[0]
except Exception as e:
yield {
"Status": "Error",
"Message": str(e)
}
return
# NOTE: Create the task and store it in tasks_dict
task = {
"R": R,
"Y": Y,
"B": B,
"well": selected_well,
"session_id": student_id,
"experiment_id": experiment_id,
"status": "queued",
}
tasks_dict[(student_id, experiment_id)] = task # Keep track globally
# Put only (student_id, experiment_id) in the Queue
task_queue.put((student_id, experiment_id))
queue_counter += 1
update_used_wells([selected_well])
decrement_student_quota(student_id)
print(f"Task added: {task}")
# First yield: "Queued"
yield {
"Status": "Queued",
"Position": queue_counter,
"Student ID": student_id,
"Experiment ID": experiment_id,
"Well": selected_well,
"Volumes": {"R": R, "Y": Y, "B": B}
}
# NOTE: Wait until the task's status becomes 'processing'
# This ensures we only yield "Running" when the backend actually starts the job.
while tasks_dict[(student_id, experiment_id)]["status"] == "queued":
time.sleep(20)
# Second yield: "Running" (happens only after status is 'processing')
yield {
"Status": "Running",
"Student ID": student_id,
"Experiment ID": experiment_id,
"Well": selected_well,
"Volumes": {"R": R, "Y": Y, "B": B}
}
# Finally, wait for the result
result = result_queue.get()
yield result
def debug_experiment(student_id, R, Y, B):
if student_id != "debug":
return {"Status": "Error", "Message": "Invalid debug request"}
experiment_id = "debug-" + secrets.token_hex(4)
yield {
"Status": "Queued",
"Position": "debug",
"Student ID": student_id,
"Experiment ID": experiment_id,
"Well": "DEBUG-A1",
"Volumes": {"R": R, "Y": Y, "B": B}
}
time.sleep(1)
yield {
"Status": "Running",
"Student ID": student_id,
"Experiment ID": experiment_id,
"Well": "DEBUG-A1",
"Volumes": {"R": R, "Y": Y, "B": B}
}
time.sleep(1)
result_debug = {
"Status": "Complete",
"Message": "Debug mode - simulated result (no actual experiment performed)",
"Student ID": student_id,
"Command": {
"R": R,
"Y": Y,
"B": B,
"well": "DEBUG-A1"
},
"Sensor Data": {
"ch583": 2800,
"ch670": 3000,
"ch510": 1700,
"ch410": 240,
"ch620": 3900,
"ch470": 1000,
"ch550": 2400,
"ch440": 900
},
"Experiment ID": experiment_id
}
yield result_debug
with gr.Blocks(title="OT-2 Liquid Color Matching Experiment Queue") as demo:
gr.Markdown("## OT-2 Liquid Color Matching Experiment Queue")
gr.Markdown("Enter R, Y, and B volumes (in µL). Total volume must not exceed 300 µL.(a volume of exactly 300 µL is recommended)")
with gr.Row():
with gr.Column(scale=2):
with gr.Row():
student_id_input = gr.Textbox(
label="Student ID",
placeholder="Enter your unique ID"
)
verify_id_btn = gr.Button("Verify ID")
r_slider = gr.Slider(0, 300, step=1, label="Red (R) Volume (µL)", interactive=False)
y_slider = gr.Slider(0, 300, step=1, label="Yellow (Y) Volume (µL)", interactive=False)
b_slider = gr.Slider(0, 300, step=1, label="Blue (B) Volume (µL)", interactive=False)
status_output = gr.Textbox(label="Status")
submit_btn = gr.Button("Submit Experiment", interactive=False)
result_output = gr.JSON(label="Experiment Status")
with gr.Column(scale=1):
gr.Markdown("### Queue Status")
queue_status = gr.Markdown("Loading queue status...")
update_status_btn = gr.Button("Refresh Queue Status")
gr.Markdown("### YouTube Livestream")
def refresh_iframe():
video_id = get_latest_video_id(channel_id, playlist_id = PLAYLIST_ID)
print(f"[DEBUG]video id: {video_id}")
return f'''
<div style="position: relative; width: 100%; padding-top: 56.25%;">
<iframe
style="position: absolute; top: 0; left: 0; width: 100%; height: 100%;"
src="https://www.youtube.com/embed/{video_id}"
title="OT-2 Livestream"
frameborder="0"
allow="accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share"
referrerpolicy="strict-origin-when-cross-origin"
allowfullscreen>
</iframe>
</div>
'''
iframe_component = gr.HTML(refresh_iframe())
refresh_video_btn = gr.Button("Refresh Livestream")
refresh_video_btn.click(fn=refresh_iframe, outputs=iframe_component)
verify_id_btn.click(
verify_student_id,
inputs=[student_id_input],
outputs=[r_slider, y_slider, b_slider, status_output, submit_btn],
api_name="verify_student_id"
)
r_slider.change(
update_status_with_queue,
inputs=[r_slider, y_slider, b_slider],
outputs=[status_output, submit_btn]
)
y_slider.change(
update_status_with_queue,
inputs=[r_slider, y_slider, b_slider],
outputs=[status_output, submit_btn]
)
b_slider.change(
update_status_with_queue,
inputs=[r_slider, y_slider, b_slider],
outputs=[status_output, submit_btn]
)
# NOTE: concurrency_limit=3 is preserved; no changes here
submit_btn.click(
add_to_queue,
inputs=[student_id_input, r_slider, y_slider, b_slider],
outputs=result_output,
api_name="submit",
concurrency_limit=8
).then(
update_queue_display,
None,
queue_status
)
update_status_btn.click(
update_queue_display,
None,
queue_status,
api_name="update_queue_display"
)
demo.load(
update_queue_display,
None,
queue_status
)
debug_btn = gr.Button("Debug Submit", visible=False)
debug_btn.click(
debug_experiment,
inputs=[student_id_input, r_slider, y_slider, b_slider],
outputs=result_output,
api_name="debug"
)
demo.queue
if __name__ == "__main__":
demo.launch() |