data/rag/knowledge_base/Cronobacter/turicensis.json

{
  "schema_version": "1.0",
  "level": "species",
  "genus": "Cronobacter",
  "species": "turicensis",

  "overview": {
    "short": "Cronobacter turicensis is a Gram-negative, facultatively anaerobic rod occasionally implicated in neonatal and adult infections.",
    "clinical_context": "Less frequently isolated but associated with invasive disease in neonates."
  },

  "expected_fields": {
    "Gram Stain": "Negative",
    "Shape": "Rods",
    "Motility": "Positive",
    "Capsule": "Variable",
    "Spore Formation": "Negative",
    "Haemolysis": "Negative",
    "Haemolysis Type": "None",
    "Media Grown On": [
      "MacConkey Agar",
      "Blood Agar"
    ],
    "Colony Morphology": [
      "Smooth",
      "Grey",
      "Moist"
    ],
    "Oxygen Requirement": "Facultative Anaerobe",
    "Growth Temperature": "6//45",
    "Catalase": "Positive",
    "Oxidase": "Negative",
    "Indole": "Negative",
    "Urease": "Negative",
    "Citrate": "Positive",
    "Methyl Red": "Variable",
    "VP": "Positive",
    "H2S": "Negative",
    "DNase": "Negative",
    "ONPG": "Positive",
    "Coagulase": "Negative",
    "Gelatin Hydrolysis": "Variable",
    "Esculin Hydrolysis": "Variable",
    "Nitrate Reduction": "Positive",
    "NaCl Tolerant (>=6%)": "Negative",
    "Lipase Test": "Negative",
    "Lysine Decarboxylase": "Positive",
    "Ornithine Decarboxylase": "Variable",
    "Ornitihine Decarboxylase": "Variable",
    "Arginine dihydrolase": "Negative",
    "Glucose Fermentation": "Positive",
    "Lactose Fermentation": "Variable",
    "Sucrose Fermentation": "Positive",
    "Maltose Fermentation": "Positive",
    "Mannitol Fermentation": "Variable",
    "Sorbitol Fermentation": "Negative",
    "Xylose Fermentation": "Positive",
    "Rhamnose Fermentation": "Variable",
    "Arabinose Fermentation": "Positive",
    "Raffinose Fermentation": "Variable",
    "Trehalose Fermentation": "Positive",
    "Inositol Fermentation": "Negative",
    "Gas Production": "Variable",
    "TSI Pattern": "A/A",
    "Colony Pattern": "Unknown",
    "Pigment": "None",
    "Motility Type": "Peritrichous"
  },

  "species_markers": [
    {
      "field": "Sorbitol Fermentation",
      "value": "Negative",
      "importance": "medium",
      "notes": "Helps differentiate from C. malonaticus."
    }
  ],

  "common_confusions": [
    {
      "species": "Cronobacter sakazakii",
      "reason": "Shared neonatal association and overlapping biochemical profile."
    }
  ],

  "when_to_question_identification": [
    "Rhamnose positivity may suggest C. malonaticus.",
    "Indole positivity suggests non-Cronobacter species."
  ],

  "recommended_next_tests": [
    {
      "test": "API 20E",
      "reason": "Enterobacterales biochemical confirmation.",
      "api_kit": "API 20E"
    },
    {
      "test": "MALDI-TOF MS",
      "reason": "Reliable discrimination within the Cronobacter genus.",
      "api_kit": "Not applicable"
    }
  ]
}