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"schema_version": "1.0",
"level": "species",
"genus": "Bacillus",
"species": "thuringiensis",
"overview": {
"short": "Bacillus thuringiensis is a motile, Gram-positive spore-forming rod known for producing parasporal crystal toxins.",
"clinical_context": "Primarily agricultural and environmental; rare human infections."
},
"expected_fields": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Media Grown On": [
"Blood Agar"
],
"Colony Morphology": [
"Large",
"Irregular",
"Haemolytic"
],
"Oxygen Requirement": "Aerobic",
"Growth Temperature": "10//45",
"Catalase": "Positive",
"Oxidase": "Variable",
"Indole": "Negative",
"Urease": "Variable",
"Citrate": "Variable",
"Methyl Red": "Variable",
"VP": "Positive",
"H2S": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Variable",
"Nitrate Reduction": "Variable",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Glucose Fermentation": "Variable",
"Lactose Fermentation": "Negative",
"Sucrose Fermentation": "Variable",
"Maltose Fermentation": "Variable",
"Mannitol Fermentation": "Variable",
"Sorbitol Fermentation": "Negative",
"Xylose Fermentation": "Variable",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Variable",
"Raffinose Fermentation": "Negative",
"Trehalose Fermentation": "Variable",
"Inositol Fermentation": "Negative",
"Gas Production": "Variable",
"TSI Pattern": "Variable",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "Peritrichous"
},
"species_markers": [
{
"field": "Parasporal crystals",
"value": "Present",
"importance": "critical",
"notes": "Defining feature distinguishing B. thuringiensis from B. cereus."
}
],
"common_confusions": [
{
"species": "Bacillus cereus",
"reason": "Nearly identical biochemically; requires crystal detection or molecular testing."
}
],
"when_to_question_identification": [
"Absence of crystal inclusions suggests B. cereus.",
"Non-haemolytic phenotype suggests B. anthracis."
],
"recommended_next_tests": [
{
"test": "Phase-contrast microscopy",
"reason": "Detects parasporal crystal inclusions."
},
{
"test": "MALDI-TOF MS",
"reason": "Differentiates species within the Bacillus cereus group."
}
]
} |