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{
"schema_version": "1.0",
"level": "species",
"genus": "Enterococcus",
"species": "faecalis",
"overview": {
"short": "Enterococcus faecalis is a Gram-positive, facultatively anaerobic coccus and the most commonly isolated Enterococcus species in human infections.",
"clinical_context": "Frequently associated with urinary tract infections, endocarditis, wound infections, and intra-abdominal infections."
},
"expected_fields": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Haemolysis": "Variable",
"Haemolysis Type": "Variable",
"Media Grown On": [
"Blood Agar",
"Bile Esculin Agar"
],
"Colony Morphology": [
"Small",
"Grey",
"Smooth"
],
"Oxygen Requirement": "Facultative Anaerobe",
"Growth Temperature": "10//45",
"Catalase": "Negative",
"Oxidase": "Negative",
"Indole": "Negative",
"Urease": "Negative",
"Citrate": "Negative",
"Methyl Red": "Positive",
"VP": "Negative",
"H2S": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Positive",
"Nitrate Reduction": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Positive",
"Glucose Fermentation": "Positive",
"Lactose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Variable",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Variable",
"Trehalose Fermentation": "Positive",
"Inositol Fermentation": "Negative",
"Gas Production": "Negative",
"TSI Pattern": "Unknown",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "None"
},
"species_markers": [
{
"field": "Arginine dihydrolase",
"value": "Positive",
"importance": "high",
"notes": "Helps distinguish E. faecalis from E. faecium."
},
{
"field": "Gelatin Hydrolysis",
"value": "Positive",
"importance": "medium",
"notes": "More commonly positive in E. faecalis than other Enterococcus species."
}
],
"common_confusions": [
{
"species": "Enterococcus faecium",
"reason": "Closely related species with overlapping biochemical profiles."
}
],
"when_to_question_identification": [
"Arginine dihydrolase negativity suggests E. faecium.",
"Atypical sugar fermentation patterns warrant confirmation.",
"Identification based solely on hemolysis pattern."
],
"recommended_next_tests": [
{
"test": "Arginine dihydrolase",
"reason": "Key discriminator from E. faecium.",
"api_kit": "API Strep"
},
{
"test": "MALDI-TOF MS",
"reason": "Accurate species-level identification.",
"api_kit": "Not applicable"
}
]
} |