BactKing / data /llm_gold_examples.json
EphAsad's picture
Update data/llm_gold_examples.json
186a43a verified
[
{
"name": "Pseudomonas aeruginosa",
"input": "Gram-negative rods observed. Catalase positive; oxidase also positive. Colonies show classic metallic sheen; green pigmentation; slightly raised; fruity odour. No haemolysis on blood agar; haemolysis type none. Indole negative. Grows well at 20//42. Media used: Blood Agar; Nutrient Agar; MacConkey Agar. Motile with polar flagella. Capsule production variable. No spores. Aerobic profile typical. MR/VP both negative. Citrate utilisation positive. Urease negative. H2S negative. Lactose fermentation negative; glucose fermentation negative; sucrose fermentation negative. Nitrate reduction positive. LDC, ODC and ADH all negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) variable. Lipase test positive. Xylose, rhamnose, mannitol, sorbitol all negative. Maltose negative. Arabinose negative. Raffinose negative. Inositol negative. Trehalose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Metallic Sheen; Green Pigmentation; Slightly Raised; Fruity Odour",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "Blood Agar; Nutrient Agar; MacConkey Agar",
"Motility": "Positive",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus aureus",
"input": "Gram-positive cocci in clusters. Catalase positive; oxidase negative. Colonies: golden; smooth; round; buttery texture. Haemolysis positive with clear beta pattern. Indole negative. Temperature range about 20//40. Plated on Blood Agar; Nutrient Agar; Mannitol Salt Agar. Non-motile. Capsule variable. No spores. Facultative anaerobe. MR negative, VP positive. Citrate negative. Urease positive. H2S negative. Lactose fermentation variable; glucose fermentation strongly positive; sucrose fermentation positive. Nitrate reduction positive. LDC, ODC, ADH all negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. No growth with >=6% NaCl beyond MSA selectivity (variable as usual). Lipase positive. Xylose negative; rhamnose negative; mannitol positive; sorbitol variable. Maltose positive. Arabinose negative. Raffinose variable. Inositol negative. Trehalose positive. Coagulase positive.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Golden; Smooth; Round; Buttery Texture",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//40",
"Media Grown On": "Blood Agar; Nutrient Agar; Mannitol Salt Agar",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Variable",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Variable",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Variable",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Positive"
}
},
{
"name": "Escherichia coli",
"input": "Typical Gram-negative rods. Catalase +, oxidase −. Colonies appear grey; smooth; moist. Haemolysis generally negative; haemolysis type none. Indole strongly positive. Temperature range 20//44. Media used today: Blood Agar; MacConkey Agar; Nutrient Agar. Motility positive. Capsule variable. No spores. Facultative anaerobe. MR positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermenter strong; glucose fermentation positive; sucrose negative. Nitrate reduction positive. LDC positive; ODC variable; ADH negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG positive. NaCl tolerance (>=6%) negative. Lipase negative. Xylose negative; rhamnose positive; mannitol positive; sorbitol positive. Maltose positive. Arabinose positive. Raffinose negative. Inositol negative. Trehalose positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Grey; Smooth; Moist",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//44",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Variable",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Positive",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Clostridium perfringens",
"input": "Gram-positive rods, boxcar-shaped. Catalase negative; oxidase negative. Colonies: greyish; spreading; irregular edges; double zone of haemolysis. Haemolysis positive; haemolysis type beta. Indole negative. Optimal growth around 20//45. Plates used: Blood Agar; Anaerobic Blood Agar; Egg Yolk Agar. Motility negative. Capsule positive. Spore formation positive. Strict anaerobe. MR positive, VP negative. Citrate negative. Urease negative. H2S positive. Lactose negative; glucose positive; sucrose positive. Nitrate reduction variable. LDC negative; ODC negative; ADH negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test positive. Xylose negative; rhamnose negative; mannitol positive; sorbitol variable. Maltose positive. Arabinose negative. Raffinose negative. Inositol negative. Trehalose positive. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Greyish; Spreading; Irregular Edges; Double Zone Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//45",
"Media Grown On": "Blood Agar; Anaerobic Blood Agar; Egg Yolk Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Positive",
"Oxygen Requirement": "Anaerobic",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Variable",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Variable",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Candida albicans",
"input": "Yeast cells, Gram-positive on stain. Catalase positive; oxidase negative. Colonies described as creamy; smooth; pasty; white. No haemolysis; haemolysis type none. Indole negative. Growth temperature around 20//42. Cultured on Sabouraud Agar; Blood Agar; Chromogenic Candida Agar. Motility negative. Capsule variable. Spore formation negative (true spores absent; chlamydospores present but not scored). Oxygen requirement aerobic. MR, VP, citrate all negative. Urease negative. H2S negative. No lactose, glucose is strongly positive, sucrose variable. Nitrate reduction negative. LDC/ODC/ADH all negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) variable. Lipase test positive. Xylose negative; rhamnose negative; mannitol positive; sorbitol positive. Maltose positive. Arabinose negative. Raffinose negative. Inositol negative. Trehalose positive. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Yeast",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Creamy; Smooth; Pasty; White",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "Sabouraud Agar; Blood Agar; Chromogenic Candida Agar",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Variable",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Klebsiella pneumoniae",
"input": "GNR, classic Kleb appearance. Catalase +. Oxidase −. Colonies very mucoid; glistening; grey-beige; high convex profile. No haemolysis noted; haemolysis type none. Indole −. Grows between 20//37. Cultured on Blood Agar; MacConkey Agar; Nutrient Agar. Non-motile as expected. Capsule very evident (positive). No spores. Facultative anaerobe. MR −, VP +. Citrate +. Urease + (moderate). H2S −. Lactose fermentation + (nice pink MAC). Glucose +, sucrose +. Nitrate reduction +. LDC +, ODC −, ADH −. Gelatin hydrolysis −. Esculin hydrolysis −. DNase −. ONPG +. NaCl ≥6%: variable. Lipase −. Xylose −; rhamnose +; mannitol +; sorbitol +; maltose +. Arabinose variable. Raffinose +. Inositol −. Trehalose +. Coagulase −.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Mucoid; Glistening; Grey-Beige; High Convex",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Variable",
"Raffinose Fermentation": "Positive",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Listeria monocytogenes",
"input": "Gram-positive rods (short, sometimes coccobacillary). Catalase +, oxidase −. Colony morphology: small; grey-white; smooth; narrow zone β-haemolysis. Haemolysis +, haemolysis type beta. Indole −. Growth observed at 4–40°C, so reporting 4//40. Media grown on Blood Agar; PALCAM Agar; Nutrient Agar. Motility + (umbrella pattern in semisolid). Capsule −. Spore formation −. Facultative anaerobe. MR −, VP +. Citrate −. Urease −. H2S −. Lactose −; glucose +; sucrose +. Nitrate reduction −. LDC −; ODC +; ADH −. Gelatin hydrolysis −. Esculin hydrolysis +. DNase −. ONPG −. NaCl tolerance up to 10% but using your scale: positive for >=6%. Lipase −. Xylose −; rhamnose +; mannitol −; sorbitol −. Maltose +. Arabinose −. Raffinose −. Inositol −. Trehalose +. Coagulase −.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Grey-White; Smooth; Narrow Beta Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "4//40",
"Media Grown On": "Blood Agar; PALCAM Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Proteus mirabilis",
"input": "GNR with classic swarming noted on BA. Catalase +, oxidase −. Colonies: spreading; grey; concentric rings. Haemolysis −; haemolysis type none. Indole − (consistent with mirabilis). Temperature tolerance 20//37. Media used: Blood Agar; MacConkey Agar; Nutrient Agar. Strongly motile. Capsule variable. No spores. Facultative anaerobe. MR +, VP −. Citrate +. Urease strongly +. H2S +. Lactose −; glucose +; sucrose +. Nitrate reduction +. LDC −; ODC +; ADH +. Gelatin hydrolysis +. Esculin hydrolysis −. DNase −. ONPG −. NaCl ≥6%: negative. Lipase −. Xylose −; rhamnose −; mannitol +; sorbitol variable. Maltose +. Arabinose −. Raffinose −. Inositol −. Trehalose +. Coagulase −.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Spreading; Grey; Concentric Rings",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Positive",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Variable",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Acinetobacter baumannii",
"input": "Gram-negative coccobacilli (short rods). Catalase +. Oxidase −. Colonies appear opaque; smooth; slightly sticky; beige. No haemolysis; haemolysis type none. Indole −. Temperature growth range tested 20//37. Media: Blood Agar; MacConkey Agar; Nutrient Agar. Non-motile. Capsule variable. Spore formation −. Aerobic. MR −, VP −. Citrate +. Urease + (usually weak). H2S −. Lactose −; glucose −; sucrose −. Nitrate reduction variable. LDC −; ODC −; ADH −. Gelatin hydrolysis −. Esculin hydrolysis −. DNase −. ONPG −. NaCl tolerance (>=6%) variable. Lipase −. Xylose −; rhamnose −; mannitol −; sorbitol −. Maltose −. Arabinose −. Raffinose −. Inositol −. Trehalose −. Coagulase −.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Short Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Opaque; Smooth; Slightly Sticky; Beige",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Variable",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pyogenes",
"input": "Gram-positive cocci in chains. Catalase −. Oxidase −. Colonies: small; translucent; dryish; strong β-haemolysis. Haemolysis +, haemolysis type beta. Indole −. Growth around 20//37. Media used: Blood Agar; Todd-Hewitt Agar; Nutrient Agar. Motility −. Capsule +. Spore formation −. Facultative anaerobe. MR −; VP −. Citrate −. Urease −. H2S −. Lactose −; glucose +; sucrose +. Nitrate reduction −. LDC −; ODC −; ADH −. Gelatin hydrolysis −. Esculin hydrolysis −. DNase −. ONPG −. NaCl ≥6%: negative. Lipase −. Xylose −; rhamnose −; mannitol −; sorbitol −. Maltose +. Arabinose −. Raffinose −. Inositol −. Trehalose +. Coagulase −.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Translucent; Dryish; Strong Beta Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; Todd-Hewitt Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Salmonella enterica",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colony morphology: smooth; grey; moist; slightly raised. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate positive. Urease negative. H2S positive. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerant (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose fermentation negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Grey; Moist; Slightly Raised",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Gram-positive cocci in pairs or short chains. Catalase negative. Oxidase negative. Colony morphology: creamy; smooth; round. Haemolysis positive, alpha-haemolysis. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; Nutrient Agar; MRS Agar. Non-motile. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP positive. Citrate negative. Urease negative. H2S negative. Lactose fermentation positive. Glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase positive. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis positive. DNase negative. ONPG negative. NaCl tolerant (>=6%) positive. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation positive. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Creamy; Smooth; Round",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; Nutrient Agar; MRS Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Positive",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus subtilis",
"input": "Gram-positive rods. Catalase positive. Oxidase negative. Colonies large; rough; irregular; cream-coloured; dry. No haemolysis, haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on Nutrient Agar; MacConkey Agar; Blood Agar. Motility positive. Capsule negative. Spore formation positive. Oxygen requirement aerobic. Methyl Red negative, VP negative. Citrate positive. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Rough; Irregular; Cream-Coloured; Dry",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Nutrient Agar; MacConkey Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pneumoniae",
"input": "Gram-positive diplococci, lancet-shaped. Catalase negative. Oxidase negative. Colonies: small; grey; alpha-haemolysis, slim zone. Haemolysis +, haemolysis type alpha. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; Nutrient Agar; MHA. Non-motile. Capsule positive. Spore formation negative. Facultative anaerobe. Methyl Red negative; VP positive. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. LDC −, ODC −, ADH −. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase −. ONPG −. NaCl ≥6%: negative. Lipase negative. Xylose −; rhamnose +; mannitol −; sorbitol negative. Maltose +. Arabinose negative. Raffinose negative. Inositol negative. Trehalose −. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Grey; Alpha-Haemolysis; Slim Zone",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; Nutrient Agar; MHA",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Clostridium difficile",
"input": "Gram-positive, anaerobic rods. Catalase negative. Oxidase negative. Colony morphology: yellow; round; moist; irregular edges. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; CCFA Agar; Nutrient Agar. Motility negative. Capsule negative. Spore formation positive. Oxygen requirement anaerobic. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation negative. Sucrose fermentation negative. Nitrate reduction negative. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis positive. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose fermentation negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Yellow; Round; Moist; Irregular Edges",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; CCFA Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Anaerobic",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus agalactiae",
"input": "Gram-positive cocci in chains. Catalase negative. Oxidase negative. Colonies: small; round; white; non-haemolytic. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; MacConkey Agar; Nutrient Agar. Non-motile. Capsule positive. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP positive. Citrate negative. Urease negative. H2S negative. Lactose fermentation positive. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) positive. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; White; Non-Haemolytic",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterobacter aerogenes",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colonies: moist; pale yellow; smooth; slightly raised. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP positive. Citrate positive. Urease negative. H2S negative. Lactose fermentation positive. Glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase positive. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG positive. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation positive. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation positive. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Moist; Pale Yellow; Smooth; Slightly Raised",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Positive",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Vibrio cholerae",
"input": "Gram-negative curved rods. Catalase positive. Oxidase positive. Colonies: smooth; moist; greenish-yellow. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Thiosulfate Citrate Bile Salts Agar (TCBS). Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate positive. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG positive. NaCl tolerance (>=6%) positive. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Smooth; Moist; Greenish-Yellow",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Thiosulfate Citrate Bile Salts Agar (TCBS)",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas fluorescens",
"input": "Gram-negative rods. Catalase positive. Oxidase positive. Colonies: large; smooth; greenish-blue pigment; shiny. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement aerobic. Methyl Red negative, VP positive. Citrate positive. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test positive. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Large; Smooth; Greenish-Blue Pigment; Shiny",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus epidermidis",
"input": "Gram-positive cocci in clusters. Catalase positive. Oxidase negative. Colony morphology: small; white; smooth; non-haemolytic. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; Nutrient Agar; Mannitol Salt Agar. Non-motile. Capsule positive. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP negative. Citrate negative. Urease positive. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction negative. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) positive. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; White; Smooth; Non-Haemolytic",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; Nutrient Agar; Mannitol Salt Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Escherichia coli O157:H7",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colonies: large; smooth; pale; non-hemolytic. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//44. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Smooth; Pale; Non-Hemolytic",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//44",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Vibrio parahaemolyticus",
"input": "Gram-negative curved rods. Catalase positive. Oxidase positive. Colonies: round; moist; greenish-blue; beta-haemolysis on TCBS Agar. Haemolysis positive, haemolysis type beta. Indole negative. Growth temperature 20//37. Media grown on MacConkey Agar; TCBS Agar; Nutrient Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate positive. Urease negative. H2S positive. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. ONPG positive. NaCl tolerance (>=6%) positive. Lipase test positive. Xylose fermentation positive. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Round; Moist; Greenish-Blue; Beta-Haemolysis on TCBS Agar",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; TCBS Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Positive",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas aeruginosa",
"input": "Gram-negative rods. Catalase positive. Oxidase positive. Colonies: large; smooth; greenish-blue pigmentation; metallic sheen. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement aerobic. Methyl Red negative, VP positive. Citrate positive. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Large; Smooth; Greenish-Blue Pigmentation; Metallic Sheen",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Candida albicans",
"input": "Yeast cells, Gram-positive on stain. Catalase positive. Oxidase negative. Colonies creamy; smooth; pasty; white. No haemolysis; haemolysis type none. Indole negative. Growth temperature 20//42. Media grown on Sabouraud Agar; Blood Agar; Chromogenic Candida Agar. Motility negative. Capsule variable. Spore formation negative (true spores absent, chlamydospores present). Oxygen requirement aerobic. MR negative, VP negative, citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation variable. Nitrate reduction negative. LDC negative, ODC negative, ADH negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) variable. Lipase positive. Xylose negative, rhamnose negative, mannitol positive, sorbitol positive. Maltose positive. Arabinose negative. Raffinose negative. Inositol negative. Trehalose positive. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Yeast",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Creamy; Smooth; Pasty; White",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "Sabouraud Agar; Blood Agar; Chromogenic Candida Agar",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Variable",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Klebsiella oxytoca",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colonies large; mucoid; smooth; pale yellowish. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility negative. Capsule positive. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP positive. Citrate positive. Urease negative. H2S negative. Lactose fermentation positive. Glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Mucoid; Smooth; Pale Yellowish",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus cereus",
"input": "Gram-positive rods. Catalase positive. Oxidase negative. Colonies large; rough; irregular; beige or white. Haemolysis positive, beta-haemolysis. Indole negative. Growth temperature 20//37. Media grown on Nutrient Agar; MacConkey Agar; Blood Agar. Motility positive. Capsule negative. Spore formation positive. Oxygen requirement aerobic. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test positive. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Rough; Irregular; Beige or White",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Nutrient Agar; MacConkey Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Shigella flexneri",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colonies small; smooth; translucent. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility negative. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation positive. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Smooth; Translucent",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Haemophilus influenzae",
"input": "Gram-negative, pleomorphic coccobacilli. Catalase positive. Oxidase positive. Colonies are small, moist, and non-haemolytic, exhibiting a smooth texture with a characteristic greyish coloration. No haemolysis is observed, and the haemolysis type is classified as none. Indole production is negative. The bacterium demonstrates growth across a temperature range of 20 to 37°C, with optimal growth conditions at 37°C. Cultivation was successful on Chocolate Agar, Blood Agar, and MacConkey Agar. Motility is absent, which is consistent with the genus. Capsule presence is negative. Spore formation is also negative. Haemophilus influenzae requires a supplemental factor, specifically factor V (nicotinamide adenine dinucleotide), which can be observed in its growth patterns. Facultative anaerobe, capable of anaerobic and aerobic growth. Methyl Red test yields a negative result, whereas the Voges-Proskauer test is also negative. The bacterium is citrate negative, and urease negative. H2S production is absent. Lactose fermentation is negative, while glucose fermentation is positive, producing acid. Nitrate reduction is evident, with the reduction of nitrate to nitrite. Lysine decarboxylase, ornithine decarboxylase, and arginine dihydrolase tests all yield negative results. Gelatin hydrolysis is negative, as is esculin hydrolysis. DNase and ONPG tests both return negative results. NaCl tolerance is negative at concentrations greater than 6%. Lipase production is not detected. Fermentation of sugars such as xylose, rhamnose, mannitol, and sorbitol is absent. Maltose fermentation is positive, and arabinose fermentation is negative. Raffinose and inositol fermentation are both negative. The bacterium exhibits no growth on trehalose. Coagulase test is negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Coccobacilli",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Small; Moist; Non-Haemolytic; Smooth; Greyish",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Chocolate Agar; Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus aureus",
"input": "Gram-positive cocci arranged in clusters, characteristic of staphylococci. Catalase positive. Oxidase negative. Colonies on Blood Agar are golden-yellow; smooth, and often exhibit beta-haemolysis. Haemolysis is positive, with beta-haemolysis type. Indole negative. Growth temperature range of 18 to 45°C, with an optimal growth temperature of 37°C. Cultured on Blood Agar, MacConkey Agar, and Mannitol Salt Agar. Motility is negative. Capsule is positive, which contributes to its virulence. Spore formation is negative. The bacterium thrives in both aerobic and anaerobic environments, being facultative. Methyl Red test positive, while VP is negative. Citrate negative. Urease positive, although weak. H2S negative. Lactose fermentation is weakly positive, glucose fermentation is positive, and sucrose fermentation is positive. Nitrate reduction is positive. LDC negative, ODC positive, ADH positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase production is strongly positive. ONPG positive. NaCl tolerance at 6% is positive. Lipase production is negative. Fermentation of sugars such as xylose, rhamnose, mannitol, and sorbitol is positive. Maltose fermentation is also positive. Arabinose fermentation negative. Raffinose and inositol are both negative. Trehalose fermentation positive. Coagulase positive, a key factor in its pathogenicity.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Golden-Yellow; Smooth; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "18//45",
"Media Grown On": "Blood Agar; MacConkey Agar; Mannitol Salt Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Weakly Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Positive"
}
},
{
"name": "Bacillus subtilis",
"input": "Gram-positive rods, forming endospores. Catalase positive. Oxidase negative. Colonies are large, rough, irregular, and opaque with a beige color. Haemolysis negative. Haemolysis type none. Indole negative. Growth temperature 20//37, with optimum growth at 30°C. Cultured on Nutrient Agar; Blood Agar; MacConkey Agar. Motility positive. Capsule negative. Spore formation positive, a distinguishing feature of this genus. Oxygen requirement aerobic. Methyl Red negative, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive, but weak. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Rough; Irregular; Beige",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Nutrient Agar; Blood Agar; MacConkey Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Shigella dysenteriae",
"input": "Gram-negative rods, facultative anaerobic. Catalase positive. Oxidase negative. Colonies are small; greyish; slightly raised; smooth. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//37. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility negative. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation positive. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish; Slightly Raised; Smooth",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Lactobacillus rhamnosus",
"input": "Gram-positive, non-spore forming rods. Catalase negative. Oxidase negative. Colonies are small, smooth, and typically white with a slightly raised profile. Haemolysis is absent, and the haemolysis type is none. Indole production is negative. Growth temperature ranges from 20°C to 42°C, with 37°C being optimal. Grows well on MRS Agar, Nutrient Agar, and Blood Agar. Motility is negative. Capsule production is negative. Spore formation is absent. Oxygen requirement is facultative anaerobic, capable of growth in both aerobic and anaerobic environments. Methyl Red test negative, VP positive. Citrate test negative. Urease production is negative. H2S production is absent. Lactose fermentation is negative, while glucose fermentation is positive, with the production of acid. Sucrose fermentation is also negative. Nitrate reduction is positive. Lysine decarboxylase negative, ornithine decarboxylase positive, and arginine dihydrolase negative. Gelatin hydrolysis is negative. Esculin hydrolysis negative. DNase production is absent. ONPG negative. NaCl tolerance at concentrations greater than 6% is negative. Lipase test is negative. Xylose fermentation is negative, rhamnose fermentation positive, mannitol fermentation negative, sorbitol negative. Maltose fermentation positive. Arabinose fermentation negative, raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase is negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Smooth; White; Slightly Raised",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "MRS Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Mycobacterium tuberculosis",
"input": "Gram-positive, acid-fast bacilli. Catalase positive. Oxidase negative. Colonies are rough; dry; non-pigmented. Haemolysis is negative, and haemolysis type is none. Indole negative. Growth temperature ranges from 30°C to 37°C. Cultured on Lowenstein-Jensen Agar, Blood Agar, and Middlebrook 7H10 Agar. Non-motile. Capsule and spore formation are absent. Mycobacterium tuberculosis requires oxygen for growth (obligate aerobe). Methyl Red and VP tests are both negative. Citrate and urease negative. H2S production is absent. Lactose fermentation is negative, glucose fermentation negative. Sucrose fermentation is negative. Nitrate reduction is positive. Lysine decarboxylase negative, ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis is negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation is negative, rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose fermentation negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Bacilli",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Rough; Dry; Non-Pigmented",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "30//37",
"Media Grown On": "Lowenstein-Jensen Agar; Blood Agar; Middlebrook 7H10 Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Escherichia coli O157:H7",
"input": "Gram-negative rods. Catalase positive. Oxidase negative. Colonies are smooth; round; pale; non-haemolytic. Haemolysis negative. Haemolysis type none. Indole positive. Growth temperature 20//44. Media grown on MacConkey Agar; Nutrient Agar; Blood Agar. Motility positive. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Round; Pale; Non-Haemolytic",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//44",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Gram-positive cocci in pairs or short chains. Catalase negative. Oxidase negative. Colonies: creamy; smooth; round. Haemolysis positive, alpha-haemolysis. Indole negative. Growth temperature 20//37. Media grown on Blood Agar; Nutrient Agar; MRS Agar. Non-motile. Capsule negative. Spore formation negative. Oxygen requirement facultative anaerobe. Methyl Red negative, VP positive. Citrate negative. Urease negative. H2S negative. Lactose fermentation positive. Glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) positive. Lipase test negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Creamy; Smooth; Round",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; Nutrient Agar; MRS Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pneumoniae",
"input": "Gram positive, lancet shaped cocci, pairs or chains, catalase neg, oxidase neg. Colonies: small, round, greyish-white, some alpha haemolysis on blood agar. Growth temp: 20 to 37. Blood agar, MacConkey agar, and nutrient agar for growth. Non-motile, capsule positive (important for virulence). Spore formation: negative. Facultative anaerobe. Methyl Red neg, VP pos. Citrate neg, urease neg. H2S not observed. Lactose neg, glucose positive. Sucrose neg, nitrate reduction pos. Lysine decarboxylase neg, ornithine decarboxylase neg. Not sure about arginine dihydrolase. Gelatin hydrolysis negative. Esculin neg. DNase neg. ONPG neg. NaCl tolerance at 6% neg. No lipase activity. Xylose fermentation neg, rhamnose neg, mannitol pos, sorbitol neg. Maltose fermenter. Arabinose and raffinose negative. Inositol neg. Trehalose neg. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Greyish-White; Alpha Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Salmonella enterica",
"input": "Gram negative rods, catalase pos, oxidase neg. Colonies on MacConkey agar: smooth, pale, non-lactose fermenter. Swarming growth on blood agar. Motility pos, hydrogen sulfide pos. Urease neg, methyl red pos. Citrate neg, VP neg. Growth temp 10-40, grows best at 37. Media used: MacConkey Agar, XLD Agar, Nutrient Agar. Capsule: no, spore formation: no. Facultative anaerobe. Lactose fermentation neg, glucose fermentation pos, sucrose fermentation neg. Nitrate reduction pos. H2S production pos, urease neg. Sucrose fermentation neg. Lysine decarboxylase neg, ornithine decarboxylase pos, arginine dihydrolase pos. Gelatin hydrolysis neg. Esculin neg, DNase neg. ONPG neg. NaCl tolerance at 6% neg. Xylose fermentation neg, rhamnose neg, mannitol pos, sorbitol neg. Arabinose and raffinose neg. Inositol fermentation neg.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "10//40",
"Media Grown On": "MacConkey Agar; XLD Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecium",
"input": "Gram-positive cocci, chains. Catalase negative, oxidase negative. Colonies: small, greyish, smooth. Haemolysis: alpha-haemolysis on blood agar. Growth at 37°C, optimal. Media: MRS agar, blood agar, nutrient agar. Non-motile, capsule absent. No spore formation. Facultative anaerobe, grows in both oxygen and anaerobic conditions. Methyl red negative, VP positive, citrate negative. Urease negative, H2S negative. Lactose fermentation negative, glucose positive. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. No lipase activity. Xylose and mannitol fermenters. Sorbitol and maltose non-fermenters. Raffinose and arabinose negative. Trehalose positive.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish; Smooth",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MRS Agar; Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Lactococcus lactis",
"input": "Gram-positive cocci, pairs or chains. Catalase negative. Oxidase negative. Colonies small, smooth, white to cream. No haemolysis observed. Methyl Red test negative. VP positive. Grows best at 30°C on MRS Agar, Blood Agar, and Nutrient Agar. Non-motile, capsule absent, spore formation negative. Facultative anaerobe. Lactose fermentation positive. Glucose fermentation positive, sucrose fermentation negative. Nitrate reduction negative. Lysine decarboxylase negative. Ornithine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. ONPG negative. NaCl tolerance (>=6%) negative. Lipase negative. Xylose fermentation negative. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose fermentation negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Smooth; White to Cream",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//30",
"Media Grown On": "MRS Agar; Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus cereus",
"input": "Gram-positive rods, chains, catalase positive, oxidase negative. Colonies large, irregular, dry, off-white to beige. Often beta-hemolytic on blood agar. No growth at 4°C. Growth at 37°C optimal. Temp range 15–45°C. Motility positive, spore forming (sometimes looks like 'hay-fever' fuzzy). Capsule negative. Lactose fermentation negative, glucose fermentation positive. Urease negative, H2S negative, nitrate reduction positive. No gelatin hydrolysis, esculin hydrolysis negative. DNase and ONPG negative. Does well in the presence of NaCl (6%), lipase negative. Mannitol fermentation positive, sorbitol negative. Maltose, arabinose fermentation negative. Xylose fermentation negative. Coagulase negative. The strain may be a major foodborne pathogen.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Irregular; Dry; Beige to Off-White",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus aureus",
"input": "Gram-positive cocci, clusters, catalase positive, oxidase negative. Colonies creamy to golden yellow, smooth, medium size, beta-hemolytic on blood agar. Growth temp 15–45°C, but best at 37°C. Capsule present (important for virulence). Non-motile, no spores. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease positive (weak). H2S negative, lactose fermentation positive, glucose fermentation positive, sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive, ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase production positive. NaCl tolerance (>=6%) positive. Lipase production negative. Xylose, mannitol fermentation positive, sorbitol negative. Maltose positive. No growth on arabinose. Trehalose fermentation negative. Coagulase positive.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Creamy to Golden Yellow; Smooth; Beta-Haemolytic",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Positive"
}
},
{
"name": "Bacillus subtilis",
"input": "Gram-positive rods, spore forming. Catalase positive, oxidase negative. Colonies are rough, dry, irregular, off-white. Beta-hemolytic on blood agar. Grows at 30°C well, temp range 20–45°C. Non-motile, capsule negative, spores present. Facultative anaerobe. Methyl Red negative, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative, glucose fermentation weakly positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. NaCl tolerance at 6% negative. Lipase negative. Xylose negative, rhamnose negative, mannitol positive, sorbitol negative. Maltose positive. Arabinose negative. Raffinose negative. Trehalose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Rough; Dry; Irregular; Off-White",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas fluorescens",
"input": "Gram-negative rods, catalase positive, oxidase positive. Colonies are large, smooth, shiny, and pale. Non-lactose fermenter, beta-hemolytic on blood agar. Growth at 25–37°C. Optimal growth at 30°C. Media: MacConkey, Blood Agar, Nutrient Agar. Motility positive, but no capsule or spore formation. Facultative anaerobe, thrives in oxygen-rich environments. Methyl Red positive, VP negative. Citrate positive. Urease negative. H2S negative. Lactose negative, glucose positive. Nitrate reduction positive. Lysine decarboxylase negative, ornithine decarboxylase positive. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. NaCl tolerance (>=6%) negative. Lipase positive. Xylose fermentation negative. Rhamnose positive. Mannitol fermentation positive. Sorbitol negative. Maltose positive. Arabinose negative. Raffinose negative. Inositol negative. Trehalose positive. Coagulase negative.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Large; Smooth; Shiny; Pale",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "25//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Positive",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Positive",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus aureus",
"input": "Gram-positive cocci in clusters, demonstrating typical staphylococcal morphology. Catalase test positive, oxidase test negative. Colonies are golden-yellow, smooth, and glossy with clear, well-defined edges. Exhibits beta-hemolysis on blood agar, characteristic of this species. The organism is non-motile and non-spore-forming, with a positive coagulase test. Growth temperature ranges from 15°C to 45°C, with optimal growth at 37°C. Media used: MacConkey Agar, Nutrient Agar, and Mannitol Salt Agar. Ferments glucose, mannitol, and maltose, but not lactose or sucrose. Methyl Red positive, VP negative, indicating a mixed acid fermentation pathway. Urease negative. Nitrate reduction occurs, and lysine decarboxylase is positive. Exhibits variable resistance to NaCl (positive at 6%), which is characteristic of this species' ability to tolerate higher salt concentrations. It is resistant to antibiotics such as penicillin due to the production of beta-lactamase. Confirmed as a pathogenic isolate with possible implications for wound infections and sepsis.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Golden-Yellow; Smooth; Glossy; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Mannitol Salt Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Positive"
}
},
{
"name": "Escherichia coli O157:H7",
"input": "Gram-negative, facultative anaerobic rods. Catalase positive, oxidase negative. Colonies on MacConkey Agar: pale, non-lactose fermenting, smooth, with occasional green sheen. The bacterium demonstrates a typical indole-positive reaction and positive growth at 37°C. Non-motile, capsule absent, and no spore formation. Pathogenic strain associated with foodborne outbreaks and hemolytic uremic syndrome. Methyl Red positive, VP negative, indicating mixed acid fermentation. Urease negative. Nitrate reduction positive. Lactose fermentation negative, glucose fermentation positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. NaCl tolerance positive at 6%. The strain produces shiga-like toxins responsible for its pathogenicity in the human intestinal tract. Negative for H2S production, sorbitol fermentation negative. Positive for maltose fermentation and trehalose fermentation. This strain should be considered a potential contaminant in food processing.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Pale; Non-Lactose Fermenting; Smooth; Green Sheen",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Positive",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Gram-positive cocci, typically in pairs or short chains, catalase negative, oxidase negative. Colonies creamy, smooth, round, with a slight sheen. Exhibits alpha-haemolysis on blood agar. The bacterium is facultative anaerobic and non-motile. Capsule negative, no spore formation. Methyl Red negative, VP positive, citrate negative. Lactose fermentation positive, glucose fermentation positive. Urease negative, H2S negative. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. On MacConkey Agar, the bacterium grows well, forming small, pale colonies. NaCl tolerance at 6.5% positive. It is resistant to vancomycin, commonly found in wound and urinary tract infections.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Creamy; Smooth; Round; Slight Sheen",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Listeria monocytogenes",
"input": "Gram-positive, non-spore-forming rods. Catalase positive. Oxidase negative. Colonies: small, smooth, greyish-white, often exhibiting narrow zone of beta-hemolysis on blood agar. Growth occurs at temperatures ranging from 4°C to 45°C, with optimal growth at 37°C. Facultative anaerobe, motility is present at 25°C but not at 37°C. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S production absent. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis positive. DNase negative. ONPG negative. NaCl tolerance (>=6%) positive. Lipase test negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Trehalose negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Smooth; Greyish-White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "4//45",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Positive at 25°C",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pneumoniae",
"input": "Gram-positive cocci, pairs or chains, diplococcal morphology typical. Catalase negative, oxidase negative. Colonies are small, round, glistening, and alpha-haemolytic on blood agar with a characteristic greenish discoloration due to partial hemolysis of red blood cells. The organism grows best at 37°C, with a temperature range of 20–42°C. MRS Agar, Blood Agar, and MacConkey Agar used for cultivation. Non-motile, capsule-positive. No spore formation. Facultative anaerobe. Methyl Red test positive, VP test negative. Citrate negative, urease negative. H2S production absent. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction is positive. Lysine decarboxylase negative, ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol negative. Maltose fermentation positive. Arabinose fermentation negative. Raffinose fermentation negative. Inositol fermentation negative. Trehalose negative. Coagulase negative. This strain is frequently isolated in respiratory infections, especially pneumonia.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Glistening; Alpha-Haemolytic",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "MRS Agar; Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas aeruginosa",
"input": "Gram-negative rods, catalase positive, oxidase positive. Colonies are large, smooth, and greenish-blue, producing a characteristic metallic sheen on nutrient agar. No haemolysis observed on blood agar. Growth temperature range 15–42°C, with optimal growth at 37°C. Motility is positive. Non-capsulated, non-spore forming. Facultative anaerobe, though prefers aerobic conditions. Methyl Red test negative, VP test positive, indicating mixed acid fermentation. Citrate positive. Urease negative. H2S production negative. Lactose fermentation negative. Glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative, ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. ONPG negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative, rhamnose fermentation negative, mannitol fermentation positive, sorbitol negative. Maltose fermentation negative. Arabinose fermentation negative. Raffinose fermentation negative. Inositol negative. Trehalose positive. The strain is often isolated from wound infections, especially burns.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Large; Smooth; Greenish-Blue; Metallic Sheen",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//42",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Escherichia coli",
"input": "Gram-negative, facultative anaerobic rods. Colonies on MacConkey Agar: round, smooth, pale with no metallic sheen. Lactose fermenter, typically producing acid and gas. Catalase positive, oxidase negative. Non-motile, capsule absent, no spore formation. Growth temperature 20–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation positive, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative, ornithine decarboxylase negative. Arginine dihydrolase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance at 6% negative. Xylose fermentation positive. Rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation positive. Maltose positive. Arabinose negative. Raffinose negative. Inositol negative. Trehalose negative. Coagulase negative. This strain is used as a model organism in molecular biology and microbiology.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Round; Smooth; Pale",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//45",
"Media Grown On": "MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Listeria monocytogenes",
"input": "Gram-positive rods, non-spore forming. Catalase positive, oxidase negative. Colonies are small, greyish-white with a slight zone of beta-hemolysis on blood agar. Growth occurs between 4°C and 44°C, with optimal growth at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S production absent. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Ornithine decarboxylase negative. Arginine dihydrolase negative. Gelatin hydrolysis positive. Esculin hydrolysis positive. DNase negative. ONPG negative. NaCl tolerance positive at 6%. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose fermentation positive. Arabinose negative. Raffinose negative. Trehalose negative. Coagulase negative. Listeria monocytogenes is pathogenic and associated with listeriosis, especially in immunocompromised individuals.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish-White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "4//44",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pneumoniae",
"input": "Gram-positive cocci in pairs or chains. Catalase negative, oxidase negative. Colonies: small, round, smooth, alpha-hemolytic on blood agar. Growth temp: 20–37°C. Media: Blood Agar, MacConkey Agar, Nutrient Agar. Non-motile, capsule positive, no spores. Facultative anaerobe. Methyl Red test negative, VP test positive. Citrate and urease negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Gelatin and esculin hydrolysis negative. DNase negative. Coagulase negative. No growth at 4°C.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Smooth; Alpha-Hemolytic",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas aeruginosa",
"input": "Gram-negative rod, catalase positive, oxidase positive. Colonies: large, smooth, greenish-blue with metallic sheen. Beta-hemolytic on blood agar. Growth temperature range: 15–42°C, best at 30°C. Motility positive, non-capsulated, non-spore forming. Facultative anaerobe. Methyl Red negative, VP positive. Citrate positive, urease negative. H2S negative. Lactose negative, glucose positive. Sucrose negative. Nitrate reduction positive. Gelatin hydrolysis positive. DNase positive. NaCl tolerance (6%) negative. No lipase activity. Sorbitol, maltose, and mannitol fermentation positive.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Large; Smooth; Greenish-Blue; Metallic Sheen",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//42",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Escherichia coli",
"input": "Gram-negative rod, facultative anaerobe. Colonies on MacConkey Agar: round, smooth, pale, lactose fermenter. Catalase positive, oxidase negative. Non-motile, no capsule, no spores. Growth temp: 20–45°C, optimal at 37°C. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose positive, glucose positive. Sucrose positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation positive. Mannitol and sorbitol fermentation positive. Maltose positive. Trehalose negative. Coagulase negative. A common gut flora, often used in molecular biology as a model organism.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Round; Smooth; Pale; Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//45",
"Media Grown On": "MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Listeria monocytogenes",
"input": "Gram-positive rods, non-spore forming. Colonies small, greyish-white, beta-hemolytic on blood agar. Growth at 4–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose negative. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis positive. Esculin hydrolysis positive. DNase negative. NaCl tolerance (>=6%) positive. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. Pathogenic, causes listeriosis, particularly in immunocompromised individuals.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish-White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "4//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Clostridium perfringens",
"input": "Gram-positive, large, rod-shaped bacterium. Catalase negative, oxidase negative. Colonies: large, round, smooth, white, with a double-zone of hemolysis on blood agar. Growth temperature range: 20–45°C, optimal growth at 37°C. Non-motile, no spore formation observed under normal culture conditions. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S production positive. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation positive. Arabinose negative. Raffinose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Large; Round; Smooth; White; Double-Zone Hemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//45",
"Media Grown On": "Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Salmonella enterica",
"input": "Gram-negative rod, catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. H2S production positive, black precipitate on XLD Agar. Growth temperature: 10–43°C, with optimal growth at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose, rhamnose, mannitol, and maltose fermentation negative. Sorbitol fermentation negative. Trehalose negative. No growth at 4°C.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "10//43",
"Media Grown On": "MacConkey Agar; XLD Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Gram-positive cocci, pairs or chains. Catalase negative, oxidase negative. Colonies: small, round, creamy, with a slight sheen. Alpha-hemolysis on blood agar. Growth temperature 20–40°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate negative. Urease negative. H2S negative. Lactose fermentation positive, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) positive. Lipase negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose positive. Arabinose negative. Raffinose negative. Inositol fermentation negative. Trehalose fermentation negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Creamy; Slight Sheen",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//40",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus subtilis",
"input": "Gram-positive rods, spore-forming. Catalase positive, oxidase negative. Colonies: large, rough, dry, beige to off-white, with irregular edges. Beta-hemolytic on blood agar. Growth temperature range: 15–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose negative. Mannitol positive. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Rough; Dry; Beige to Off-White",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Listeria monocytogenes",
"input": "Gram-positive rods, non-spore forming. Catalase positive, oxidase negative. Colonies small, greyish-white with a beta-hemolytic zone on blood agar. Growth at 4–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis positive. Esculin hydrolysis positive. DNase negative. NaCl tolerance (>=6%) positive. Xylose fermentation negative, rhamnose negative, mannitol positive. Sorbitol negative, maltose positive. Arabinose and raffinose fermentation negative. Inositol negative. Trehalose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish-White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "4//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus epidermidis",
"input": "Gram-positive cocci, clusters. Catalase positive, oxidase negative. Colonies small, white to off-white, smooth, non-hemolytic. Growth temperature range: 15–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate negative, urease negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance positive at 6%. Xylose fermentation negative, rhamnose positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Trehalose fermentation negative. Coagulase negative. Common skin commensal, opportunistic pathogen.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Small; White to Off-White; Smooth",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Gram-positive cocci, pairs or chains. Catalase negative, oxidase negative. Colonies: small, round, creamy, slightly raised, with a slight sheen. Alpha-hemolysis observed on blood agar. Growth temperature: 20–37°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate negative, urease negative. Lactose fermentation positive, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) positive. Lipase negative. Xylose fermentation negative, rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Trehalose negative. Coagulase negative. Commonly found in urinary tract infections.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Creamy; Slightly Raised",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus subtilis",
"input": "Gram-positive, spore-forming rods. Catalase positive, oxidase negative. Colonies: rough, dry, irregular, beige to off-white. Beta-hemolytic on blood agar. Growth temperature: 15–45°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative, rhamnose negative. Mannitol fermentation positive. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Rough; Dry; Irregular; Beige to Off-White",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pyogenes",
"input": "Gram-positive cocci, chains. Catalase negative, oxidase negative. Colonies: small, round, smooth, white, with a clear zone of beta-hemolysis on blood agar. Growth temperature range: 35–37°C, optimal growth at 37°C. Non-motile, non-spore-forming. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative. Urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. This strain is a major human pathogen causing diseases such as pharyngitis, scarlet fever, and necrotizing fasciitis.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Round; Smooth; White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "35//37",
"Media Grown On": "Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Clostridium difficile",
"input": "Gram-positive, anaerobic, rod-shaped bacterium. Catalase negative, oxidase negative. Colonies: greyish-white, irregular, with a rough texture. Exhibits double-zone of hemolysis on blood agar. Growth temperature range: 30–40°C, optimal at 37°C. Non-motile, spore-forming, which is important for its survival in the environment. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative, rhamnose fermentation negative. Mannitol fermentation negative, sorbitol fermentation negative. Maltose positive. Trehalose negative. Coagulase negative. This strain is known for its role in hospital-acquired infections, particularly in the colon, causing antibiotic-associated diarrhea.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Greyish-White; Irregular; Rough; Double-Zone Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "30//40",
"Media Grown On": "Blood Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus anthracis",
"input": "Gram-positive rods, spore-forming. Colonies on nutrient agar: large, round, irregular, rough, and grey-white with a central spore. Exhibits gamma hemolysis (no hemolysis) on blood agar. Growth temperature range: 18–40°C, with optimal growth at 37°C. Non-motile, no capsule. Facultative anaerobe. Methyl Red test positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Arabinose fermentation negative. Raffinose negative. Inositol negative. Trehalose negative. Coagulase negative. Pathogenic strain causing anthrax in animals and humans.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Round; Irregular; Rough; Grey-White; Central Spore",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "18//40",
"Media Grown On": "Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus aureus",
"input": "Gram-positive cocci in clusters. Catalase positive, oxidase negative. Colonies are golden-yellow, smooth, and glossy, with beta-hemolysis on blood agar. Growth temperature: 15–45°C, optimal at 37°C. Non-motile, capsule-positive. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease positive. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase positive. NaCl tolerance (>=6%) positive. Xylose fermentation negative, rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Trehalose negative. Coagulase positive. A common pathogen causing wound infections, pneumonia, and sepsis.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Golden-Yellow; Smooth; Glossy; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Blood Agar; MacConkey Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Positive"
}
},
{
"name": "Klebsiella pneumoniae",
"input": "Gram-negative, non-motile rods. Catalase positive, oxidase negative. Colonies on MacConkey Agar: large, smooth, mucoid, lactose fermenting. On blood agar, colonies are creamy white and show alpha-hemolysis. Growth temperature range: 20–37°C, optimal at 37°C. Facultative anaerobe, capsule positive, no spore formation. Methyl Red negative, VP positive. Citrate positive, urease positive. H2S negative. Lactose fermentation positive, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation positive, rhamnose fermentation negative. Mannitol fermentation negative, sorbitol fermentation negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. This organism is commonly associated with pneumonia, urinary tract infections, and bloodstream infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Smooth; Mucoid; Lactose Fermenting",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Positive",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus agalactiae",
"input": "Gram-positive cocci, pairs or chains. Catalase negative, oxidase negative. Colonies on blood agar are small, greyish-white with beta-hemolysis. On MacConkey Agar, colonies are pale and non-lactose fermenting. Growth temperature: 30–37°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis positive. DNase negative. NaCl tolerance (>=6%) positive. Xylose fermentation negative, rhamnose fermentation positive. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. A major cause of neonatal sepsis and meningitis.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Small; Greyish-White; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "30//37",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterobacter cloacae",
"input": "Gram-negative, motile rods. Catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, lactose fermenting. On blood agar, colonies are creamy white, non-hemolytic. Growth temperature: 18–40°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate positive, urease negative. H2S production absent. Lactose fermentation positive, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose, rhamnose, and mannitol fermentation positive. Sorbitol negative. Maltose positive. Trehalose positive. Coagulase negative. This bacterium is commonly associated with urinary tract and respiratory infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Lactose Fermenting",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "18//40",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Positive",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas putida",
"input": "Gram-negative rod, catalase positive, oxidase positive. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. Exhibits no hemolysis on blood agar. Growth temperature: 15–37°C, optimal at 30°C. Facultative anaerobe, motile. Methyl Red test negative, VP positive. Citrate positive. Urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase positive. NaCl tolerance (>=6%) negative. Xylose, rhamnose, and mannitol fermentation negative. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. This bacterium is commonly isolated from water and soil, and is used in environmental and industrial bioremediation.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//37",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Enterobacter aerogenes",
"input": "Gram-negative, motile rods. Catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, lactose fermenting. On blood agar, colonies are creamy white, non-hemolytic. Growth temperature range: 18–40°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate positive, urease negative. H2S negative. Lactose fermentation positive, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation positive, rhamnose fermentation negative. Mannitol fermentation positive, sorbitol fermentation negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. This strain is often found in urinary tract infections and respiratory infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Lactose Fermenting",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "18//40",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Bacillus cereus",
"input": "Gram-positive, spore-forming rods. Catalase positive, oxidase negative. Colonies on nutrient agar: large, rough, dry, and cream to yellow in color. Beta-hemolysis on blood agar. Growth temperature range: 15–45°C, optimal at 30°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. NaCl tolerance (>=6%) negative. Xylose fermentation negative, rhamnose fermentation negative. Mannitol fermentation positive. Sorbitol fermentation negative. Maltose positive. Trehalose negative. Coagulase negative. Known for food poisoning outbreaks due to toxin production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Large; Rough; Dry; Cream to Yellow; Beta-Haemolysis",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "15//45",
"Media Grown On": "Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Salmonella enteritidis",
"input": "Gram-negative rods, catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. Exhibits H2S production on XLD Agar, black colonies. Growth temperature: 10–43°C, optimal at 37°C. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase positive. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose fermentation positive. Arabinose negative. Raffinose negative. Coagulase negative. This strain is associated with foodborne infections, especially from contaminated poultry and eggs.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenting; H2S Positive",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "10//43",
"Media Grown On": "MacConkey Agar; XLD Agar; Nutrient Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Positive",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Positive",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas fluorescens",
"input": "Gram-negative rod, catalase positive, oxidase positive. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. Exhibits no hemolysis on blood agar. Growth temperature: 5–35°C, optimal at 25°C. Non-motile. Facultative anaerobe. Methyl Red test negative, VP positive. Citrate positive, urease negative. H2S production absent. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose, rhamnose, and mannitol fermentation negative. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. Found in water, soil, and plants, commonly used in bioremediation.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "5//35",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Acinetobacter baumannii",
"input": "Gram-negative coccobacilli, catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. No hemolysis on blood agar. Growth temperature range: 20–37°C, optimal at 37°C. Non-motile, non-spore forming. Facultative anaerobe. Methyl Red negative, VP negative. Citrate positive, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative, rhamnose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. Known for its resistance to antibiotics and its role in hospital-acquired infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Coccobacilli",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//37",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Shigella dysenteriae",
"input": "Gram-negative rod, catalase positive, oxidase negative. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. On blood agar: colonies are small, grey, non-hemolytic. Growth temperature: 37°C. Non-motile, no capsule, no spore formation. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation positive. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose positive. Trehalose negative. Coagulase negative. Pathogen responsible for bacillary dysentery, primarily affecting the intestines.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "37",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Neisseria gonorrhoeae",
"input": "Gram-negative diplococci, catalase positive, oxidase positive. Colonies on chocolate agar: round, smooth, moist, creamy-white. Growth temperature: 35–37°C, with an optimal growth at 37°C in a CO2-enriched atmosphere. Non-motile, no capsule, no spore formation. Facultative anaerobe. Methyl Red negative, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation negative. Sorbitol fermentation negative. Maltose fermentation negative. Trehalose negative. Coagulase negative. This pathogen causes gonorrhea and is a major sexually transmitted infection.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Round; Smooth; Moist; Creamy-White",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "35//37",
"Media Grown On": "Chocolate Agar; Nutrient Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Streptococcus pneumoniae",
"input": "Gram-positive, alpha-hemolytic cocci in pairs or chains. Catalase negative, oxidase negative. Colonies on blood agar: round, smooth, mucoid, greyish-white. Growth temperature range: 30–37°C, optimal at 37°C. Non-motile, no spore formation. Facultative anaerobe. Methyl Red positive, VP negative. Citrate negative, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation positive. Sorbitol negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. Common cause of pneumonia, otitis media, and meningitis.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Round; Smooth; Mucoid; Greyish-White",
"Haemolysis": "Positive",
"Haemolysis Type": "Alpha",
"Indole": "Negative",
"Growth Temperature": "30//37",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Pseudomonas aeruginosa",
"input": "Gram-negative rods, catalase positive, oxidase positive. Non-lactose fermenter. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. Exhibits a characteristic blue-green pigment on nutrient agar due to pyocyanin production. On blood agar, colonies are large, round, and beta-hemolytic. Growth temperature range: 20–42°C, optimal at 37°C. Motile. Facultative anaerobe. Methyl Red negative, VP negative. Citrate positive, urease negative. H2S negative. Glucose fermentation positive, but no fermentation of other sugars (e.g. sucrose, lactose). Nitrate reduction positive. Gelatin hydrolysis positive. Esculin hydrolysis negative. DNase positive. NaCl tolerance (>=6%) positive. Xylose, rhamnose, and mannitol fermentation negative. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. A major pathogen in cystic fibrosis and wound infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter; Blue-Green Pigment",
"Haemolysis": "Positive",
"Haemolysis Type": "Beta",
"Indole": "Negative",
"Growth Temperature": "20//42",
"Media Grown On": "MacConkey Agar; Nutrient Agar; Blood Agar",
"Motility": "Positive",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Positive",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Acinetobacter baumannii",
"input": "Gram-negative, non-motile rods. Catalase positive, oxidase negative. Non-lactose fermenter. Colonies on MacConkey Agar: smooth, pale, non-lactose fermenting. No hemolysis on blood agar. Growth temperature range: 20–40°C, optimal at 37°C. Facultative anaerobe. Methyl Red negative, VP positive. Citrate positive, urease negative. H2S negative. Glucose fermentation positive, but no other sugar fermentation observed. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) negative. Xylose fermentation negative. Mannitol fermentation negative, sorbitol fermentation negative. Maltose fermentation positive. Trehalose negative. Coagulase negative. Known for its resistance to antibiotics and its role in hospital-acquired infections.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Smooth; Pale; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "20//40",
"Media Grown On": "MacConkey Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Moraxella catarrhalis",
"input": "Gram-negative diplococci, catalase positive, oxidase positive. Non-lactose fermenter. Colonies on chocolate agar: smooth, greyish, moist. No hemolysis on blood agar. Growth temperature: 35–37°C, optimal at 37°C. Non-motile, non-spore forming. Facultative anaerobe. Methyl Red negative, VP negative. Citrate positive, urease negative. H2S negative. Lactose fermentation negative, glucose fermentation positive. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) positive. Xylose fermentation negative, mannitol fermentation negative. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. This pathogen is often found in respiratory tract infections, particularly in children and the elderly.",
"expected": {
"Gram Stain": "Negative",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Positive",
"Colony Morphology": "Smooth; Greyish; Moist; Non-Lactose Fermenter",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "35//37",
"Media Grown On": "Chocolate Agar; Blood Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Positive",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Staphylococcus saprophyticus",
"input": "Gram-positive cocci, clusters. Catalase positive, oxidase negative. Non-lactose fermenter. Colonies on blood agar: round, smooth, white to off-white with no hemolysis. Growth temperature range: 30–40°C, optimal at 37°C. Non-motile, non-spore forming. Facultative anaerobe. Methyl Red negative, VP positive. Citrate negative, urease positive. H2S negative. Lactose fermentation negative, glucose fermentation positive. Sucrose fermentation negative. Nitrate reduction positive. Lysine decarboxylase negative. Gelatin hydrolysis negative. Esculin hydrolysis negative. DNase negative. NaCl tolerance (>=6%) positive. Xylose fermentation negative, mannitol fermentation positive. Sorbitol negative. Maltose positive. Trehalose negative. Coagulase negative. Common cause of urinary tract infections, particularly in sexually active women.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Round; Smooth; White to Off-White; Non-Hemolytic",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "30//40",
"Media Grown On": "Blood Agar; MacConkey Agar",
"Motility": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Negative",
"VP": "Positive",
"Citrate": "Negative",
"Urease": "Positive",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Positive",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Coagulase": "Negative"
}
},
{
"name": "Weissella soli",
"input": "Gram-positive cocci observed. Catalase and oxidase both negative. Colonies on MRS agar are white, smooth, and circular with a smooth overall colony pattern. No pigment observed. Fermented odor present. No haemolysis detected; haemolysis type none. Indole negative. Growth observed between 15 and 37°C. Non-motile organism; motility type negative. Capsule and spore formation not observed. Facultative anaerobe. Methyl Red positive, Voges–Proskauer negative. Citrate and urease negative. No H2S production. Ferments lactose, glucose, and sucrose. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylation reactions negative. Gelatin not hydrolysed. Esculin hydrolysis positive. DNase negative. ONPG positive. No growth in ≥6% NaCl. Lipase negative. Xylose, mannitol, maltose, arabinose, and trehalose fermented; rhamnose, sorbitol, raffinose, and inositol not fermented. Coagulase negative. TSI shows A/A with gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "White; Smooth; Circular",
"Colony Pattern": "Smooth",
"Pigment": "None",
"Odor": "Fermented",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//37",
"Media Grown On": "MRS Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Positive",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "A/A",
"Gas Production": "Positive"
}
},
{
"name": "Weissella kandleri",
"input": "Gram-positive cocci identified. Catalase and oxidase reactions negative. Colonies grown on MRS agar are cream coloured, smooth, and convex with a smooth colony pattern. No pigment detected. Fermented odor present. No haemolysis observed. Indole negative. Growth noted from 15 to 40°C. Organism is non-motile with no capsule or spore formation. Facultative anaerobic growth. Methyl Red positive and VP negative. Citrate and urease negative. H2S not produced. Lactose, glucose, and sucrose fermented. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylase reactions negative. Gelatin hydrolysis negative. Esculin hydrolysis positive. DNase negative. ONPG positive. No tolerance to ≥6% NaCl. Lipase negative. Xylose, mannitol, maltose, arabinose, and trehalose fermented; rhamnose, sorbitol, raffinose, and inositol not fermented. Coagulase negative. TSI demonstrates A/A with gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Cream; Smooth; Convex",
"Colony Pattern": "Smooth",
"Pigment": "None",
"Odor": "Fermented",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "15//40",
"Media Grown On": "MRS Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Positive",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Positive",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "A/A",
"Gas Production": "Positive"
}
},
{
"name": "Thermoactinomyces intermedius",
"input": "Gram-positive rods observed. Catalase positive and oxidase negative. Colonies on nutrient agar are white, chalky, and dry with a chalky colony pattern. No pigment noted. Musty odor present. No haemolysis observed; haemolysis type none. Indole negative. Growth occurs between 45 and 65°C. Non-motile organism with motility type negative. Capsule not detected; spore formation present. Strictly aerobic growth. Methyl Red and VP reactions negative. Citrate and urease negative. No H2S produced. Does not ferment lactose or sucrose; glucose fermentation positive. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylation reactions negative. Gelatin hydrolysis positive; esculin not hydrolysed. DNase negative. ONPG negative. No tolerance to ≥6% NaCl. Lipase negative. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol not fermented. Trehalose fermentation positive. Coagulase negative. TSI shows K/K with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "White; Chalky; Dry",
"Colony Pattern": "Chalky",
"Pigment": "None",
"Odor": "Musty",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//65",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces sacchari",
"input": "Gram-positive rods identified. Catalase positive; oxidase negative. Colonies on nutrient agar appear cream coloured, filamentous, and matte with a filamentous growth pattern. No pigment observed. Earthy odor present. No haemolysis detected. Indole negative. Growth observed between 45 and 60°C. Organism is non-motile with no capsule formation; spores present. Aerobic growth only. Methyl Red and VP negative. Citrate and urease negative. No H2S production. Lactose and sucrose not fermented; glucose fermentation positive. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylase reactions negative. Gelatin hydrolysis positive; esculin hydrolysis negative. DNase and ONPG negative. No growth in ≥6% NaCl. Lipase negative. No fermentation of xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, or inositol. Trehalose fermentation positive. Coagulase negative. TSI demonstrates K/K with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Cream; Filamentous; Matte",
"Colony Pattern": "Filamentous",
"Pigment": "None",
"Odor": "Earthy",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//60",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces putidus",
"input": "Gram-positive rods seen on microscopy. Catalase positive and oxidase negative. Colonies grown on nutrient agar are white, rough, and chalky with a rough colony pattern. No pigment detected. Musty odor present. No haemolysis observed. Indole negative. Growth noted between 50 and 70°C. Non-motile organism; motility type negative. Capsule not observed; spore formation present. Aerobic growth. Methyl Red and VP reactions negative. Citrate and urease negative. No H2S production. Lactose and sucrose not fermented; glucose fermentation positive. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylase reactions negative. Gelatin hydrolysis positive; esculin hydrolysis negative. DNase and ONPG negative. No tolerance to ≥6% NaCl. Lipase negative. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol not fermented. Trehalose fermentation positive. Coagulase negative. TSI shows K/K with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "White; Rough; Chalky",
"Colony Pattern": "Rough",
"Pigment": "None",
"Odor": "Musty",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "50//70",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces guangxiensis",
"input": "Gram-positive rods observed. Catalase positive and oxidase negative. Colonies on nutrient agar are cream coloured, dry, and irregular with a dry colony pattern. No pigment present. Earthy odor detected. No haemolysis observed. Indole negative. Growth range noted between 45 and 65°C. Organism is non-motile with no capsule formation; spores present. Aerobic growth only. Methyl Red and VP negative. Citrate and urease negative. H2S not produced. Lactose and sucrose not fermented; glucose fermentation positive. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylase reactions negative. Gelatin hydrolysis positive; esculin hydrolysis negative. DNase and ONPG negative. No tolerance to ≥6% NaCl. Lipase negative. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol not fermented. Trehalose fermentation positive. Coagulase negative. TSI shows K/K with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Cream; Dry; Irregular",
"Colony Pattern": "Dry",
"Pigment": "None",
"Odor": "Earthy",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//65",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces vulgaris",
"input": "Gram-positive rods identified. Catalase positive; oxidase negative. Colonies grown on nutrient agar are white, chalky, and spreading with a spreading colony pattern. No pigment detected. Musty odor present. No haemolysis observed. Indole negative. Growth occurs between 45 and 70°C. Organism is non-motile with no capsule formation; spore formation present. Aerobic growth. Methyl Red and VP reactions negative. Citrate and urease negative. No H2S production. Lactose and sucrose not fermented; glucose fermentation positive. Nitrate reduction negative. Lysine, ornithine, and arginine decarboxylase reactions negative. Gelatin hydrolysis positive; esculin hydrolysis negative. DNase and ONPG negative. No tolerance to ≥6% NaCl. Lipase negative. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol not fermented. Trehalose fermentation positive. Coagulase negative. TSI demonstrates K/K with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "White; Chalky; Spreading",
"Colony Pattern": "Spreading",
"Pigment": "None",
"Odor": "Musty",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//70",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces intermedius",
"input": "Microscopy demonstrated Gram-positive rods. Catalase reaction was positive; oxidase reaction was negative. Following incubation on nutrient agar, colonies were observed to be white, chalky, and dry, exhibiting a chalky colony pattern. No pigment was detected. A musty odor was noted. No haemolysis was observed; haemolysis type none. Indole test was negative. Growth was observed within a temperature range of 45 to 65°C. The organism was non-motile, with motility type negative. Capsule was not detected; spore formation was observed. Growth was strictly aerobic. Methyl Red and Voges–Proskauer tests were negative. Citrate and urease tests were negative. No hydrogen sulfide production was detected. Lactose and sucrose were not fermented; glucose fermentation was positive. Nitrate reduction was negative. Lysine, ornithine, and arginine decarboxylation reactions were negative. Gelatin hydrolysis was positive; esculin hydrolysis was negative. DNase and ONPG tests were negative. No growth was observed in media containing ≥6% NaCl. Lipase activity was not detected. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol were not fermented. Trehalose fermentation was positive. Coagulase test was negative. TSI agar demonstrated a K/K reaction with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "White; Chalky; Dry",
"Colony Pattern": "Chalky",
"Pigment": "None",
"Odor": "Musty",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//65",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Thermoactinomyces sacchari",
"input": "Gram-positive rods were observed on microscopic examination. Catalase testing was positive; oxidase testing was negative. Colonies grown on nutrient agar were cream coloured, filamentous, and matte, displaying a filamentous colony pattern. No pigment was detected. An earthy odor was noted. No haemolysis was observed. Indole test was negative. Growth was observed between 45 and 60°C. The organism was non-motile, with no capsule formation observed; spore formation was present. Growth was aerobic only. Methyl Red and Voges–Proskauer reactions were negative. Citrate and urease tests were negative. No hydrogen sulfide production was detected. Lactose and sucrose were not fermented; glucose fermentation was positive. Nitrate reduction was negative. Lysine, ornithine, and arginine decarboxylation reactions were negative. Gelatin hydrolysis was positive; esculin hydrolysis was negative. DNase and ONPG tests were negative. No growth was observed in ≥6% NaCl. Lipase activity was not detected. Xylose, rhamnose, mannitol, sorbitol, maltose, arabinose, raffinose, and inositol were not fermented. Trehalose fermentation was positive. Coagulase test was negative. TSI agar demonstrated a K/K reaction with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Rods",
"Catalase": "Positive",
"Oxidase": "Negative",
"Colony Morphology": "Cream; Filamentous; Matte",
"Colony Pattern": "Filamentous",
"Pigment": "None",
"Odor": "Earthy",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "45//60",
"Media Grown On": "Nutrient Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Positive",
"Oxygen Requirement": "Aerobic",
"Methyl Red": "Negative",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Negative",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Negative",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Gelatin Hydrolysis": "Positive",
"Esculin Hydrolysis": "Negative",
"DNase": "Negative",
"ONPG": "Negative",
"NaCl Tolerant (>=6%)": "Negative",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Negative",
"Sorbitol Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "K/K",
"Gas Production": "Negative"
}
},
{
"name": "Enterococcus faecalis",
"input": "Microscopic examination demonstrated Gram-positive cocci. Catalase and oxidase tests were negative. Following incubation on blood agar and bile esculin agar, colonies were observed to be grey, smooth, and circular, exhibiting a smooth colony pattern. No pigment was detected. A fermented odor was noted. No haemolysis was observed; haemolysis type none. Indole test was negative. Growth was observed between 10 and 45°C. The organism was non-motile, with motility type negative. Capsule and spore formation were not observed. Growth was consistent with a facultative anaerobe. Methyl Red test was positive; Voges–Proskauer test was negative. Citrate and urease tests were negative. No hydrogen sulfide production was detected. Lactose, glucose, and sucrose were fermented. Nitrate reduction was negative. Lysine and ornithine decarboxylase tests were negative; arginine dihydrolase was positive. Gelatin hydrolysis was negative. Esculin hydrolysis was positive. DNase test was negative. ONPG test was positive. Growth was observed in media containing ≥6% NaCl. Lipase activity was not detected. Xylose and rhamnose were not fermented. Mannitol, sorbitol, maltose, and trehalose were fermented. Arabinose, raffinose, and inositol were not fermented. Coagulase test was negative. TSI agar demonstrated an A/A reaction with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "Grey; Smooth; Circular",
"Colony Pattern": "Smooth",
"Pigment": "None",
"Odor": "Fermented",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "10//45",
"Media Grown On": "Blood Agar; Bile Esculin Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Positive",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "A/A",
"Gas Production": "Negative"
}
},
{
"name": "Enterococcus faecium",
"input": "Gram-positive cocci were observed on microscopy. Catalase and oxidase reactions were negative. Colonies grown on blood agar and bile esculin agar were white, smooth, and circular with a smooth colony pattern. No pigment was detected. A fermented odor was noted. No haemolysis was observed; haemolysis type none. Indole test was negative. Growth was observed between 10 and 45°C. The organism was non-motile, with motility type negative. Capsule and spore formation were not observed. Growth characteristics were consistent with a facultative anaerobe. Methyl Red test was positive; Voges–Proskauer test was negative. Citrate and urease tests were negative. No hydrogen sulfide production was detected. Lactose, glucose, and sucrose were fermented. Nitrate reduction was negative. Lysine and ornithine decarboxylase tests were negative; arginine dihydrolase was positive. Gelatin hydrolysis was negative. Esculin hydrolysis was positive. DNase test was negative. ONPG test was positive. Growth was observed in media containing ≥6% NaCl. Lipase activity was not detected. Xylose and rhamnose were not fermented. Mannitol, maltose, and trehalose were fermented; sorbitol fermentation was variable. Arabinose, raffinose, and inositol were not fermented. Coagulase test was negative. TSI agar demonstrated an A/A reaction with no gas production.",
"expected": {
"Gram Stain": "Positive",
"Shape": "Cocci",
"Catalase": "Negative",
"Oxidase": "Negative",
"Colony Morphology": "White; Smooth; Circular",
"Colony Pattern": "Smooth",
"Pigment": "None",
"Odor": "Fermented",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Indole": "Negative",
"Growth Temperature": "10//45",
"Media Grown On": "Blood Agar; Bile Esculin Agar",
"Motility": "Negative",
"Motility Type": "Negative",
"Capsule": "Negative",
"Spore Formation": "Negative",
"Oxygen Requirement": "Facultative Anaerobe",
"Methyl Red": "Positive",
"VP": "Negative",
"Citrate": "Negative",
"Urease": "Negative",
"H2S": "Negative",
"Lactose Fermentation": "Positive",
"Glucose Fermentation": "Positive",
"Sucrose Fermentation": "Positive",
"Nitrate Reduction": "Negative",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Arginine dihydrolase": "Positive",
"Gelatin Hydrolysis": "Negative",
"Esculin Hydrolysis": "Positive",
"DNase": "Negative",
"ONPG": "Positive",
"NaCl Tolerant (>=6%)": "Positive",
"Lipase Test": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Mannitol Fermentation": "Positive",
"Sorbitol Fermentation": "Variable",
"Maltose Fermentation": "Positive",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Trehalose Fermentation": "Positive",
"Coagulase": "Negative",
"TSI Pattern": "A/A",
"Gas Production": "Negative"
}
}
]