| { | |
| "schema_version": "1.0", | |
| "level": "species", | |
| "genus": "Acinetobacter", | |
| "species": "baumannii", | |
| "overview": { | |
| "short": "Acinetobacter baumannii is a Gram-negative, strictly aerobic, non-fermentative coccobacillus associated with severe healthcare-associated infections.", | |
| "clinical_context": "A major cause of ventilator-associated pneumonia, bloodstream infections, wound infections, and outbreaks in intensive care units." | |
| }, | |
| "expected_fields": { | |
| "Gram Stain": "Negative", | |
| "Shape": "Coccobacilli", | |
| "Motility": "Negative", | |
| "Capsule": "Variable", | |
| "Spore Formation": "Negative", | |
| "Haemolysis": "Negative", | |
| "Haemolysis Type": "None", | |
| "Media Grown On": [ | |
| "Blood Agar", | |
| "MacConkey Agar" | |
| ], | |
| "Colony Morphology": [ | |
| "Smooth", | |
| "Opaque", | |
| "Grey-White" | |
| ], | |
| "Oxygen Requirement": "Aerobic", | |
| "Growth Temperature": "20//44", | |
| "Catalase": "Positive", | |
| "Oxidase": "Negative", | |
| "Indole": "Negative", | |
| "Urease": "Variable", | |
| "Citrate": "Variable", | |
| "Methyl Red": "Negative", | |
| "VP": "Negative", | |
| "H2S": "Negative", | |
| "DNase": "Variable", | |
| "ONPG": "Negative", | |
| "Coagulase": "Negative", | |
| "Gelatin Hydrolysis": "Variable", | |
| "Esculin Hydrolysis": "Negative", | |
| "Nitrate Reduction": "Negative", | |
| "NaCl Tolerant (>=6%)": "Positive", | |
| "Lipase Test": "Variable", | |
| "Lysine Decarboxylase": "Negative", | |
| "Ornithine Decarboxylase": "Negative", | |
| "Ornitihine Decarboxylase": "Negative", | |
| "Arginine dihydrolase": "Negative", | |
| "Glucose Fermentation": "Negative", | |
| "Lactose Fermentation": "Negative", | |
| "Sucrose Fermentation": "Negative", | |
| "Maltose Fermentation": "Negative", | |
| "Mannitol Fermentation": "Variable", | |
| "Sorbitol Fermentation": "Negative", | |
| "Xylose Fermentation": "Negative", | |
| "Rhamnose Fermentation": "Negative", | |
| "Arabinose Fermentation": "Negative", | |
| "Raffinose Fermentation": "Negative", | |
| "Trehalose Fermentation": "Negative", | |
| "Inositol Fermentation": "Negative", | |
| "Gas Production": "Negative", | |
| "TSI Pattern": "K/K", | |
| "Colony Pattern": "Unknown", | |
| "Pigment": "None", | |
| "Motility Type": "None" | |
| }, | |
| "species_markers": [ | |
| { | |
| "field": "NaCl Tolerant (>=6%)", | |
| "value": "Positive", | |
| "importance": "high", | |
| "notes": "Salt tolerance supports A. baumannii over many other Acinetobacter species." | |
| }, | |
| { | |
| "field": "Growth Temperature", | |
| "value": "44//44", | |
| "importance": "high", | |
| "notes": "Growth at elevated temperatures is characteristic of A. baumannii." | |
| } | |
| ], | |
| "common_confusions": [ | |
| { | |
| "species": "Acinetobacter pittii", | |
| "reason": "Part of the A. baumannii complex with overlapping biochemical profiles." | |
| }, | |
| { | |
| "species": "Acinetobacter calcoaceticus", | |
| "reason": "Closely related environmental species within the complex." | |
| } | |
| ], | |
| "when_to_question_identification": [ | |
| "Failure to grow at elevated temperatures suggests a non-baumannii species.", | |
| "Environmental isolation without clinical context may indicate another Acinetobacter species." | |
| ], | |
| "recommended_next_tests": [ | |
| { | |
| "test": "MALDI-TOF MS", | |
| "reason": "Accurate differentiation within the Acinetobacter baumannii complex." | |
| }, | |
| { | |
| "test": "Antimicrobial susceptibility testing", | |
| "reason": "Critical due to frequent multidrug resistance." | |
| }, | |
| { | |
| "test": "Molecular identification", | |
| "reason": "Definitive species-level confirmation when clinically significant." | |
| } | |
| ] | |
| } |