| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Acinetobacter", |
| "species": "calcoaceticus", |
|
|
| "overview": { |
| "short": "Acinetobacter calcoaceticus is a Gram-negative, aerobic, non-fermentative coccobacillus primarily associated with environmental sources.", |
| "clinical_context": "Rarely implicated in human disease and most often recovered from soil, water, and hospital environments." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Coccobacilli", |
| "Motility": "Negative", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "MacConkey Agar" |
| ], |
| "Colony Morphology": [ |
| "Smooth", |
| "Grey", |
| "Dry" |
| ], |
| "Oxygen Requirement": "Aerobic", |
| "Growth Temperature": "15//37", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Negative", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Negative", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Negative", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Negative", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "K/K", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "None" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Growth Temperature", |
| "value": "37//37", |
| "importance": "high", |
| "notes": "Inability to grow at higher temperatures helps exclude pathogenic Acinetobacter species." |
| }, |
| { |
| "field": "NaCl Tolerant (>=6%)", |
| "value": "Negative", |
| "importance": "high", |
| "notes": "Lack of salt tolerance differentiates A. calcoaceticus from A. baumannii." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Acinetobacter pittii", |
| "reason": "Closely related within the A. baumannii complex, but more clinically relevant." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Isolation from severe clinical infections suggests another Acinetobacter species.", |
| "Growth at elevated temperatures argues against A. calcoaceticus." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Required to distinguish environmental Acinetobacter species." |
| } |
| ] |
| } |