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Create pittii.json
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{
"schema_version": "1.0",
"level": "species",
"genus": "Acinetobacter",
"species": "pittii",
"overview": {
"short": "Acinetobacter pittii is a Gram-negative, aerobic, non-fermentative coccobacillus belonging to the Acinetobacter baumannii complex.",
"clinical_context": "Associated with opportunistic infections including bloodstream, respiratory, and wound infections, generally less virulent than A. baumannii."
},
"expected_fields": {
"Gram Stain": "Negative",
"Shape": "Coccobacilli",
"Motility": "Negative",
"Capsule": "Variable",
"Spore Formation": "Negative",
"Haemolysis": "Negative",
"Haemolysis Type": "None",
"Media Grown On": [
"Blood Agar",
"MacConkey Agar"
],
"Colony Morphology": [
"Smooth",
"Opaque",
"Grey-White"
],
"Oxygen Requirement": "Aerobic",
"Growth Temperature": "20//40",
"Catalase": "Positive",
"Oxidase": "Negative",
"Indole": "Negative",
"Urease": "Variable",
"Citrate": "Variable",
"Methyl Red": "Negative",
"VP": "Negative",
"H2S": "Negative",
"DNase": "Variable",
"ONPG": "Negative",
"Coagulase": "Negative",
"Gelatin Hydrolysis": "Variable",
"Esculin Hydrolysis": "Negative",
"Nitrate Reduction": "Negative",
"NaCl Tolerant (>=6%)": "Variable",
"Lipase Test": "Variable",
"Lysine Decarboxylase": "Negative",
"Ornithine Decarboxylase": "Negative",
"Ornitihine Decarboxylase": "Negative",
"Arginine dihydrolase": "Negative",
"Glucose Fermentation": "Negative",
"Lactose Fermentation": "Negative",
"Sucrose Fermentation": "Negative",
"Maltose Fermentation": "Negative",
"Mannitol Fermentation": "Variable",
"Sorbitol Fermentation": "Negative",
"Xylose Fermentation": "Negative",
"Rhamnose Fermentation": "Negative",
"Arabinose Fermentation": "Negative",
"Raffinose Fermentation": "Negative",
"Trehalose Fermentation": "Negative",
"Inositol Fermentation": "Negative",
"Gas Production": "Negative",
"TSI Pattern": "K/K",
"Colony Pattern": "Unknown",
"Pigment": "None",
"Motility Type": "None"
},
"species_markers": [
{
"field": "Growth Temperature",
"value": "40//40",
"importance": "high",
"notes": "Typically does not grow at the higher temperatures tolerated by A. baumannii."
},
{
"field": "NaCl Tolerant (>=6%)",
"value": "Variable",
"importance": "medium",
"notes": "Reduced salt tolerance compared to A. baumannii."
}
],
"common_confusions": [
{
"species": "Acinetobacter baumannii",
"reason": "Part of the A. baumannii complex with overlapping biochemical characteristics."
},
{
"species": "Acinetobacter calcoaceticus",
"reason": "Genetically related environmental species within the complex."
}
],
"when_to_question_identification": [
"Growth at ≥44°C suggests A. baumannii.",
"Strong salt tolerance supports A. baumannii over A. pittii."
],
"recommended_next_tests": [
{
"test": "MALDI-TOF MS",
"reason": "Primary method for differentiating species within the A. baumannii complex."
},
{
"test": "Molecular identification",
"reason": "Definitive resolution when clinical significance is high."
}
]
}