| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Alcaligenes", |
| "species": "denitrificans", |
|
|
| "overview": { |
| "short": "Alcaligenes denitrificans is an aerobic, oxidase-positive, non-fermentative Gram-negative rod capable of nitrate reduction.", |
| "clinical_context": "Rarely isolated from clinical specimens but may be encountered in environmental or hospital settings." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Rods", |
| "Motility": "Positive", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Nutrient Agar" |
| ], |
| "Colony Morphology": [ |
| "Smooth", |
| "Grey-White" |
| ], |
| "Oxygen Requirement": "Aerobe", |
| "Growth Temperature": "20//42", |
| "Catalase": "Positive", |
| "Oxidase": "Positive", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Negative", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Positive", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Negative", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Negative", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Negative", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "K/K", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Peritrichous" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Nitrate Reduction", |
| "value": "Positive", |
| "importance": "high", |
| "notes": "Primary distinguishing feature of A. denitrificans." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Alcaligenes faecalis", |
| "reason": "Shared oxidase positivity and non-fermentative profile." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Negative nitrate reduction suggests A. faecalis.", |
| "Glucose fermentation suggests another genus." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Nitrate reduction", |
| "reason": "Defining test for this species." |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Accurate species-level confirmation." |
| } |
| ] |
| } |
