| { |
| "schema_version": "1.0", |
| "level": "genus", |
| "genus": "Alcaligenes", |
|
|
| "overview": { |
| "short": "Alcaligenes species are Gram-negative, aerobic, non-fermentative rods commonly found in environmental sources and occasionally associated with opportunistic infections.", |
| "clinical_context": "Rarely pathogenic but may be isolated from respiratory specimens, blood, or hospital environments." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Rods", |
| "Motility": "Positive", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Nutrient Agar" |
| ], |
| "Colony Morphology": [ |
| "Small", |
| "Smooth", |
| "Grey-White" |
| ], |
| "Oxygen Requirement": "Aerobe", |
| "Growth Temperature": "20//42", |
| "Catalase": "Positive", |
| "Oxidase": "Positive", |
| "Indole": "Negative", |
| "Urease": "Variable", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Variable", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Variable", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Variable", |
| "Ornitihine Decarboxylase": "Variable", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Negative", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Negative", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Negative", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "K/K", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Peritrichous" |
| }, |
|
|
| "field_notes": { |
| "Oxidase": "Strong oxidase positivity is characteristic.", |
| "Glucose Fermentation": "Alcaligenes species are non-fermentative." |
| }, |
|
|
| "key_differentiators": [ |
| { |
| "field": "Oxidase", |
| "expected": "Positive", |
| "distinguishes_from": ["Enterobacter", "Escherichia"], |
| "notes": "Separates Alcaligenes from oxidase-negative Enterobacterales." |
| }, |
| { |
| "field": "Glucose Fermentation", |
| "expected": "Negative", |
| "distinguishes_from": ["Aeromonas"], |
| "notes": "Non-fermentative metabolism is genus-defining." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "genus": "Achromobacter", |
| "reason": "Both are oxidase-positive, non-fermentative Gram-negative rods." |
| }, |
| { |
| "genus": "Pseudomonas", |
| "reason": "Shared aerobic growth and non-fermentative metabolism." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Oxidase negativity suggests another genus.", |
| "Glucose fermentation argues against Alcaligenes.", |
| "Pigment production suggests Pseudomonas." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Oxidase test", |
| "reason": "Key screening test for non-fermentative Gram-negative rods." |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Recommended due to limited biochemical resolution." |
| } |
| ], |
|
|
| "supported_species": [ |
| "faecalis", |
| "denitrificans" |
| ] |
| } |
