| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Bacteroides", |
| "species": "fragilis", |
|
|
| "overview": { |
| "short": "Bacteroides fragilis is a bile-resistant, encapsulated anaerobic Gram-negative rod.", |
| "clinical_context": "Most clinically significant Bacteroides species; commonly causes intra-abdominal abscesses and bacteraemia." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Rods", |
| "Motility": "Negative", |
| "Capsule": "Positive", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Anaerobic Blood Agar", |
| "Bacteroides Bile Esculin Agar" |
| ], |
| "Colony Morphology": [ |
| "Grey", |
| "Smooth", |
| "Convex" |
| ], |
| "Oxygen Requirement": "Obligate Anaerobe", |
| "Growth Temperature": "20//45", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Variable", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Variable", |
| "ONPG": "Positive", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Negative", |
| "Esculin Hydrolysis": "Positive", |
| "Nitrate Reduction": "Variable", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Positive", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Positive", |
| "Maltose Fermentation": "Positive", |
| "Mannitol Fermentation": "Positive", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Positive", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Positive", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Positive", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Variable", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "None" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Capsule", |
| "value": "Positive", |
| "importance": "critical", |
| "notes": "Major virulence factor unique within the genus." |
| }, |
| { |
| "field": "Bile resistance", |
| "value": "Positive", |
| "importance": "high", |
| "notes": "Growth on bile-containing media is characteristic." |
| } |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "API 20A", |
| "reason": "Species-level anaerobe identification.", |
| "api_kit": "API 20A" |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Rapid and accurate species identification." |
| } |
| ] |
| } |
