| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Borrelia", |
| "species": "afzelii", |
|
|
| "overview": { |
| "short": "Borrelia afzelii is a Lyme disease–associated spirochete prevalent in Europe.", |
| "clinical_context": "Frequently associated with chronic cutaneous manifestations such as acrodermatitis chronica atrophicans." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Spiral", |
| "Motility": "Positive", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Barbour-Stoenner-Kelly Agar" |
| ], |
| "Colony Morphology": [ |
| "Not visible on routine media" |
| ], |
| "Oxygen Requirement": "Microaerophilic", |
| "Growth Temperature": "30//34", |
| "Catalase": "Negative", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Negative", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Negative", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Negative", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Negative", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Axial Filaments" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Geographic Distribution", |
| "value": "Europe", |
| "importance": "high", |
| "notes": "Predominantly European Lyme disease agent." |
| }, |
| { |
| "field": "Clinical Manifestation", |
| "value": "Chronic cutaneous disease", |
| "importance": "medium", |
| "notes": "Skin involvement more common than neurologic disease." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Borrelia burgdorferi", |
| "reason": "Serologic cross-reactivity and overlapping Lyme disease features." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Neurologic-dominant disease may suggest other Borrelia species." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "PCR with species-specific primers", |
| "reason": "Differentiates European Lyme Borrelia species." |
| } |
| ] |
| } |
