| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Brevibacterium", |
| "species": "casei", |
|
|
| "overview": { |
| "short": "Brevibacterium casei is a Gram-positive, aerobic, non-motile coryneform bacterium commonly found on human skin.", |
| "clinical_context": "Primarily a skin commensal but may be isolated from opportunistic infections, particularly in immunocompromised patients or device-associated infections." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Short Rods", |
| "Motility": "Negative", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar" |
| ], |
| "Colony Morphology": [ |
| "Dry", |
| "Rough", |
| "Cream to light orange" |
| ], |
| "Oxygen Requirement": "Aerobic", |
| "Growth Temperature": "20//37", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Positive", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Positive", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Variable", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Variable", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Variable", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "Light orange", |
| "Motility Type": "None" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Pigment", |
| "value": "Light orange", |
| "importance": "medium", |
| "notes": "Pigmentation is present but typically less intense than B. linens." |
| }, |
| { |
| "field": "Gelatin Hydrolysis", |
| "value": "Positive", |
| "importance": "medium", |
| "notes": "Supports identification within Brevibacterium." |
| }, |
| { |
| "field": "Clinical Context", |
| "value": "Skin flora", |
| "importance": "high", |
| "notes": "Common isolation from skin supports B. casei over environmental species." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Brevibacterium linens", |
| "reason": "Shared pigmentation and biochemical overlap." |
| }, |
| { |
| "genus": "Corynebacterium", |
| "reason": "Similar coryneform morphology and catalase positivity." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Strong orange pigmentation suggests B. linens.", |
| "Strict anaerobic growth argues against Brevibacterium.", |
| "Catalase-negative result suggests Actinomyces." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "API Coryne", |
| "reason": "Useful for differentiation among coryneform bacteria." |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Preferred method for definitive species identification." |
| } |
| ] |
| } |
