| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Brevibacterium", |
| "species": "linens", |
|
|
| "overview": { |
| "short": "Brevibacterium linens is a Gram-positive, aerobic, coryneform bacterium notable for strong orange pigmentation.", |
| "clinical_context": "Commonly associated with cheese-rind microbiota and human skin; rarely implicated in opportunistic infections." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Short Rods", |
| "Motility": "Negative", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Variable", |
| "Haemolysis Type": "Variable", |
| "Media Grown On": [ |
| "Blood Agar" |
| ], |
| "Colony Morphology": [ |
| "Dry", |
| "Rough", |
| "Bright orange" |
| ], |
| "Oxygen Requirement": "Aerobic", |
| "Growth Temperature": "15//37", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Variable", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Positive", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Positive", |
| "Lipase Test": "Variable", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Variable", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Variable", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Variable", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "Orange", |
| "Motility Type": "None" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Pigment", |
| "value": "Bright orange", |
| "importance": "high", |
| "notes": "Strong orange pigmentation is characteristic of B. linens." |
| }, |
| { |
| "field": "NaCl Tolerant (>=6%)", |
| "value": "Positive", |
| "importance": "medium", |
| "notes": "High salt tolerance supports environmental and cheese-associated origin." |
| }, |
| { |
| "field": "Growth Temperature", |
| "value": "15//37", |
| "importance": "low", |
| "notes": "Ability to grow at lower temperatures supports differentiation from other skin flora." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Brevibacterium casei", |
| "reason": "Shared coryneform morphology with overlapping biochemical reactions." |
| }, |
| { |
| "genus": "Dermabacter", |
| "reason": "Similar skin-associated Gram-positive rods." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Absence of strong orange pigmentation suggests B. casei.", |
| "Poor salt tolerance argues against B. linens.", |
| "Anaerobic growth suggests alternative genera." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "API Coryne", |
| "reason": "Useful for preliminary differentiation among coryneform bacteria." |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Gold standard for accurate species-level identification." |
| } |
| ] |
| } |
