| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Brucella", |
| "species": "melitensis", |
|
|
| "overview": { |
| "short": "Brucella melitensis is a Gram-negative, non-motile coccobacillus considered the most virulent Brucella species for humans.", |
| "clinical_context": "Primarily infects goats and sheep, causing caprine brucellosis, and is a leading cause of human brucellosis worldwide." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Negative", |
| "Shape": "Short Rods", |
| "Motility": "Negative", |
| "Capsule": "Negative", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Chocolate Agar" |
| ], |
| "Colony Morphology": [ |
| "Small", |
| "Smooth", |
| "Translucent" |
| ], |
| "Oxygen Requirement": "Aerobic", |
| "Growth Temperature": "35//37", |
| "Catalase": "Positive", |
| "Oxidase": "Positive", |
| "Indole": "Negative", |
| "Urease": "Positive", |
| "Citrate": "Negative", |
| "Methyl Red": "Negative", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Negative", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Positive", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Glucose Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "Pigment": "None", |
| "Motility Type": "None" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "H2S", |
| "value": "Negative", |
| "importance": "high", |
| "notes": "Absence of H₂S differentiates B. melitensis from B. abortus." |
| }, |
| { |
| "field": "CO₂ Requirement", |
| "value": "None", |
| "importance": "medium", |
| "notes": "Does not require added CO₂ for growth, unlike some B. abortus strains." |
| }, |
| { |
| "field": "Host Association", |
| "value": "Goats and sheep", |
| "importance": "medium", |
| "notes": "Strong correlation with small ruminants in endemic regions." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Brucella abortus", |
| "reason": "Shared biochemical results aside from H₂S production." |
| }, |
| { |
| "genus": "Ochrobactrum", |
| "reason": "Phylogenetic similarity may lead to misidentification." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "H₂S positivity suggests B. abortus.", |
| "Failure to grow aerobically or absence of urease suggests misidentification.", |
| "Rapid growth or fermentation reactions inconsistent with Brucella." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "H₂S production", |
| "reason": "Confirms distinction from B. abortus and B. suis." |
| }, |
| { |
| "test": "PCR or molecular typing", |
| "reason": "Definitive confirmation due to biosafety concerns." |
| }, |
| { |
| "test": "Serological agglutination test", |
| "reason": "Supports diagnosis in clinical or veterinary contexts." |
| } |
| ] |
| } |
