| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Cellulomonas", |
| "species": "fimi", |
|
|
| "overview": { |
| "short": "Cellulomonas fimi is a Gram-positive, aerobic rod best known for strong cellulose-degrading activity.", |
| "clinical_context": "Primarily environmental; rare opportunistic infections have been reported in immunocompromised hosts." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Rods", |
| "Motility": "Positive", |
| "Capsule": "Unknown", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Nutrient Agar" |
| ], |
| "Colony Morphology": [ |
| "Smooth", |
| "Yellow", |
| "Convex" |
| ], |
| "Oxygen Requirement": "Aerobe", |
| "Growth Temperature": "20//37", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Unknown", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Positive", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Positive", |
| "Esculin Hydrolysis": "Positive", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Positive", |
| "Lactose Fermentation": "Variable", |
| "Sucrose Fermentation": "Positive", |
| "Maltose Fermentation": "Positive", |
| "Mannitol Fermentation": "Variable", |
| "Sorbitol Fermentation": "Variable", |
| "Xylose Fermentation": "Positive", |
| "Rhamnose Fermentation": "Variable", |
| "Arabinose Fermentation": "Positive", |
| "Raffinose Fermentation": "Variable", |
| "Trehalose Fermentation": "Positive", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "Yellow", |
| "Motility Type": "Peritrichous" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Motility", |
| "value": "Positive", |
| "importance": "high", |
| "notes": "Motility helps differentiate C. fimi from non-motile Cellulomonas species." |
| }, |
| { |
| "field": "ONPG", |
| "value": "Positive", |
| "importance": "medium", |
| "notes": "Supports carbohydrate metabolism consistent with C. fimi." |
| }, |
| { |
| "field": "Gelatin Hydrolysis", |
| "value": "Positive", |
| "importance": "medium", |
| "notes": "Strong gelatin hydrolysis aligns with environmental cellulose-degrading role." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Cellulomonas flavigena", |
| "reason": "Shared pigmentation and overlapping carbohydrate utilization." |
| }, |
| { |
| "genus": "Microbacterium", |
| "reason": "Similar Gram-positive rods with yellow colonies." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Non-motile isolate observed.", |
| "Oxidase positivity detected.", |
| "Spore formation present." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Motility testing", |
| "reason": "Primary discriminator between Cellulomonas species.", |
| "api_kit": "API Coryne" |
| }, |
| { |
| "test": "ONPG test", |
| "reason": "Supports carbohydrate profile confirmation.", |
| "api_kit": "API Coryne" |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Definitive identification for environmental Gram-positive rods." |
| } |
| ] |
| } |
