| { |
| "schema_version": "1.0", |
| "level": "genus", |
| "genus": "Cutibacterium", |
|
|
| "overview": { |
| "short": "Cutibacterium species are Gram-positive, anaerobic to aerotolerant short rods that are part of normal human skin flora.", |
| "clinical_context": "Most commonly associated with acne, implant-associated infections, and opportunistic deep-seated infections following disruption of skin barriers." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Short Rods", |
| "Motility": "Negative", |
| "Capsule": "Variable", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Anaerobic Blood Agar" |
| ], |
| "Colony Morphology": [ |
| "Small", |
| "White", |
| "Cream", |
| "Smooth" |
| ], |
| "Oxygen Requirement": "Anaerobic", |
| "Growth Temperature": "30//37", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Variable", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Variable", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Variable", |
| "Esculin Hydrolysis": "Negative", |
| "Nitrate Reduction": "Positive", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Positive", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Positive", |
| "Lactose Fermentation": "Negative", |
| "Sucrose Fermentation": "Negative", |
| "Maltose Fermentation": "Variable", |
| "Mannitol Fermentation": "Negative", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Negative", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Negative", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Variable", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Variable", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Unknown" |
| }, |
|
|
| "field_notes": { |
| "Oxygen Requirement": "Primarily anaerobic but some species are aerotolerant.", |
| "Lipase Test": "Characteristic feature contributing to skin lipid metabolism.", |
| "Indole": "Species-dependent and useful at species level." |
| }, |
|
|
| "key_differentiators": [ |
| { |
| "field": "Lipase Test", |
| "expected": "Positive", |
| "distinguishes_from": ["Corynebacterium"], |
| "notes": "Lipase positivity supports Cutibacterium over other Gram-positive skin flora." |
| }, |
| { |
| "field": "Nitrate Reduction", |
| "expected": "Positive", |
| "distinguishes_from": ["Actinomyces"], |
| "notes": "Helps differentiate from other anaerobic Gram-positive rods." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "genus": "Corynebacterium", |
| "reason": "Both are Gram-positive skin commensals with overlapping morphology." |
| }, |
| { |
| "genus": "Actinomyces", |
| "reason": "Shared anaerobic growth and Gram-positive rod morphology." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Strong aerobic growth without anaerobic preference suggests Corynebacterium.", |
| "Motility or spore formation argues against Cutibacterium.", |
| "Oxidase positivity is inconsistent." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Indole test", |
| "reason": "Critical for species-level differentiation.", |
| "api_kit": "API Rapid ID 32A" |
| }, |
| { |
| "test": "Lipase activity", |
| "reason": "Supports genus-level confirmation.", |
| "api_kit": "API Rapid ID 32A" |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Highly reliable for Cutibacterium species identification.", |
| "api_kit": "Not applicable" |
| } |
| ], |
|
|
| "supported_species": [ |
| "acnes", |
| "avidum", |
| "granulosum" |
| ] |
| } |
