| { |
| "schema_version": "1.0", |
| "level": "genus", |
| "genus": "Eubacterium", |
|
|
| "overview": { |
| "short": "Eubacterium species are Gram-positive, obligately anaerobic rods commonly found in the human gastrointestinal tract.", |
| "clinical_context": "Typically part of normal gut flora but may be isolated from abscesses, bloodstream infections, and polymicrobial anaerobic infections." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Rods", |
| "Motility": "Variable", |
| "Capsule": "Variable", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Anaerobic Blood Agar" |
| ], |
| "Colony Morphology": [ |
| "Small", |
| "Grey", |
| "Smooth" |
| ], |
| "Oxygen Requirement": "Anaerobic", |
| "Growth Temperature": "30//37", |
| "Catalase": "Negative", |
| "Oxidase": "Negative", |
| "Indole": "Variable", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Variable", |
| "VP": "Negative", |
| "H2S": "Variable", |
| "DNase": "Negative", |
| "ONPG": "Negative", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Variable", |
| "Esculin Hydrolysis": "Variable", |
| "Nitrate Reduction": "Variable", |
| "NaCl Tolerant (>=6%)": "Negative", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Variable", |
| "Glucose Fermentation": "Positive", |
| "Lactose Fermentation": "Variable", |
| "Sucrose Fermentation": "Variable", |
| "Maltose Fermentation": "Variable", |
| "Mannitol Fermentation": "Variable", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Variable", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Variable", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Variable", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Variable", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Unknown" |
| }, |
|
|
| "field_notes": { |
| "Oxygen Requirement": "Strict anaerobes; growth in aerobic conditions argues against Eubacterium.", |
| "Indole": "Highly species-dependent and not genus-defining.", |
| "Motility": "Variable across species and strains." |
| }, |
|
|
| "key_differentiators": [ |
| { |
| "field": "Catalase", |
| "expected": "Negative", |
| "distinguishes_from": ["Cutibacterium"], |
| "notes": "Catalase negativity helps separate Eubacterium from catalase-positive anaerobes." |
| }, |
| { |
| "field": "Oxygen Requirement", |
| "expected": "Anaerobic", |
| "distinguishes_from": ["Corynebacterium"], |
| "notes": "Strict anaerobic growth differentiates from aerotolerant Gram-positive rods." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "genus": "Clostridium", |
| "reason": "Shared anaerobic growth and Gram-positive rod morphology." |
| }, |
| { |
| "genus": "Actinomyces", |
| "reason": "Overlapping anaerobic biochemical profiles." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Spore formation suggests Clostridium.", |
| "Aerobic growth suggests Corynebacterium or related genera.", |
| "Strong catalase positivity is inconsistent." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Anaerobic identification panel", |
| "reason": "Supports biochemical profiling under anaerobic conditions.", |
| "api_kit": "API Rapid ID 32A" |
| }, |
| { |
| "test": "Indole test", |
| "reason": "Useful for species-level differentiation.", |
| "api_kit": "API Rapid ID 32A" |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Most reliable approach for definitive identification.", |
| "api_kit": "Not applicable" |
| } |
| ], |
|
|
| "supported_species": [ |
| "limosum", |
| "rectale", |
| "ventriosum" |
| ] |
| } |
