| { |
| "schema_version": "1.0", |
| "level": "species", |
| "genus": "Exiguobacterium", |
| "species": "acetylicum", |
|
|
| "overview": { |
| "short": "Exiguobacterium acetylicum is a Gram-positive, facultatively anaerobic short rod originally isolated from industrial and environmental sources.", |
| "clinical_context": "Rarely associated with human infection; most often encountered as an environmental or laboratory contaminant." |
| }, |
|
|
| "expected_fields": { |
| "Gram Stain": "Positive", |
| "Shape": "Short Rods", |
| "Motility": "Variable", |
| "Capsule": "Unknown", |
| "Spore Formation": "Negative", |
| "Haemolysis": "Negative", |
| "Haemolysis Type": "None", |
| "Media Grown On": [ |
| "Blood Agar", |
| "Nutrient Agar" |
| ], |
| "Colony Morphology": [ |
| "Small", |
| "Smooth", |
| "Cream" |
| ], |
| "Oxygen Requirement": "Facultative Anaerobe", |
| "Growth Temperature": "10//45", |
| "Catalase": "Positive", |
| "Oxidase": "Negative", |
| "Indole": "Negative", |
| "Urease": "Negative", |
| "Citrate": "Negative", |
| "Methyl Red": "Positive", |
| "VP": "Negative", |
| "H2S": "Negative", |
| "DNase": "Negative", |
| "ONPG": "Variable", |
| "Coagulase": "Negative", |
| "Gelatin Hydrolysis": "Variable", |
| "Esculin Hydrolysis": "Variable", |
| "Nitrate Reduction": "Negative", |
| "NaCl Tolerant (>=6%)": "Positive", |
| "Lipase Test": "Negative", |
| "Lysine Decarboxylase": "Negative", |
| "Ornithine Decarboxylase": "Negative", |
| "Ornitihine Decarboxylase": "Negative", |
| "Arginine dihydrolase": "Negative", |
| "Glucose Fermentation": "Positive", |
| "Lactose Fermentation": "Variable", |
| "Sucrose Fermentation": "Variable", |
| "Maltose Fermentation": "Positive", |
| "Mannitol Fermentation": "Variable", |
| "Sorbitol Fermentation": "Negative", |
| "Xylose Fermentation": "Variable", |
| "Rhamnose Fermentation": "Negative", |
| "Arabinose Fermentation": "Variable", |
| "Raffinose Fermentation": "Negative", |
| "Trehalose Fermentation": "Positive", |
| "Inositol Fermentation": "Negative", |
| "Gas Production": "Negative", |
| "TSI Pattern": "Unknown", |
| "Colony Pattern": "Unknown", |
| "Pigment": "None", |
| "Motility Type": "Variable" |
| }, |
|
|
| "species_markers": [ |
| { |
| "field": "Methyl Red", |
| "value": "Positive", |
| "importance": "medium", |
| "notes": "Supports differentiation from other Exiguobacterium species." |
| }, |
| { |
| "field": "Nitrate Reduction", |
| "value": "Negative", |
| "importance": "medium", |
| "notes": "Helps separate from nitrate-reducing species." |
| } |
| ], |
|
|
| "common_confusions": [ |
| { |
| "species": "Exiguobacterium antarcticum", |
| "reason": "Shared morphology and overlapping fermentation patterns." |
| } |
| ], |
|
|
| "when_to_question_identification": [ |
| "Strong oxidase positivity suggests an alternative genus.", |
| "Spore formation excludes Exiguobacterium.", |
| "Strict psychrophilic growth favours E. antarcticum." |
| ], |
|
|
| "recommended_next_tests": [ |
| { |
| "test": "Extended carbohydrate fermentation", |
| "reason": "Supports species-level separation.", |
| "api_kit": "API 50 CH" |
| }, |
| { |
| "test": "MALDI-TOF MS", |
| "reason": "Reliable confirmation due to biochemical overlap.", |
| "api_kit": "Not applicable" |
| } |
| ] |
| } |
