{ "schema_version": "1.0", "level": "species", "genus": "Acinetobacter", "species": "johnsonii", "overview": { "short": "Acinetobacter johnsonii is a Gram-negative, aerobic, non-fermentative coccobacillus commonly recovered from environmental sources.", "clinical_context": "Rarely associated with human infection and typically considered of low pathogenic potential." }, "expected_fields": { "Gram Stain": "Negative", "Shape": "Coccobacilli", "Motility": "Negative", "Capsule": "Negative", "Spore Formation": "Negative", "Haemolysis": "Negative", "Haemolysis Type": "None", "Media Grown On": [ "Blood Agar", "MacConkey Agar" ], "Colony Morphology": [ "Smooth", "Grey", "Dry" ], "Oxygen Requirement": "Aerobic", "Growth Temperature": "15//37", "Catalase": "Positive", "Oxidase": "Negative", "Indole": "Negative", "Urease": "Negative", "Citrate": "Negative", "Methyl Red": "Negative", "VP": "Negative", "H2S": "Negative", "DNase": "Negative", "ONPG": "Negative", "Coagulase": "Negative", "Gelatin Hydrolysis": "Negative", "Esculin Hydrolysis": "Negative", "Nitrate Reduction": "Negative", "NaCl Tolerant (>=6%)": "Negative", "Lipase Test": "Negative", "Lysine Decarboxylase": "Negative", "Ornithine Decarboxylase": "Negative", "Ornitihine Decarboxylase": "Negative", "Arginine dihydrolase": "Negative", "Glucose Fermentation": "Negative", "Lactose Fermentation": "Negative", "Sucrose Fermentation": "Negative", "Maltose Fermentation": "Negative", "Mannitol Fermentation": "Negative", "Sorbitol Fermentation": "Negative", "Xylose Fermentation": "Negative", "Rhamnose Fermentation": "Negative", "Arabinose Fermentation": "Negative", "Raffinose Fermentation": "Negative", "Trehalose Fermentation": "Negative", "Inositol Fermentation": "Negative", "Gas Production": "Negative", "TSI Pattern": "K/K", "Colony Pattern": "Unknown", "Pigment": "None", "Motility Type": "None" }, "species_markers": [ { "field": "Growth Temperature", "value": "37//37", "importance": "medium", "notes": "Does not tolerate higher temperatures typical of A. baumannii." } ], "common_confusions": [ { "species": "Acinetobacter lwoffii", "reason": "Similar low biochemical activity and environmental presence." } ], "when_to_question_identification": [ "Isolation from severe clinical infections may indicate another species.", "Growth at elevated temperatures argues against A. johnsonii." ], "recommended_next_tests": [ { "test": "MALDI-TOF MS", "reason": "Definitive species-level identification." } ] }