Update app.py

app.py

@@ -758,8 +758,6 @@ def create_gradio_interface():
 
 # --- Application Startup ---
 def run_application():
-    e = None  # Predefine e to avoid free variable error
-    fallback_error = None  # Predefine fallback_error for clarity
     gradio_app = None  # Predefine gradio_app for cleanup
     try:
         gradio_app = create_gradio_interface()
@@ -776,11 +774,12 @@ def run_application():
         )
     except Exception as e:
         logger.error(f"Application startup failed: {e}", exc_info=True)
+        fallback_gradio_app = None  # Predefine for fallback
         try:
             logger.info("🔄 Falling back to Gradio-only mode...")
-            gradio_app = create_gradio_interface()
+            fallback_gradio_app = create_gradio_interface()
             logger.warning("⚠️ Running without request queuing in fallback mode.")
-            gradio_app.launch(
+            fallback_gradio_app.launch(
                 server_name="0.0.0.0",
                 server_port=7860,
                 share=False,
@@ -790,18 +789,17 @@ def run_application():
             logger.error(f"Fallback failed: {fallback_error}", exc_info=True)
             print("❌ Application failed to start. Check logs for details.")
         finally:
-            if gradio_app:
+            if fallback_gradio_app:
                 try:
-                    gradio_app.close()
+                    fallback_gradio_app.close()
                 except Exception as close_error:
-                    logger.warning(f"Failed to close Gradio app in fallback: {close_error}")
+                    logger.warning(f"Failed to close fallback Gradio app: {close_error}")
     finally:
         if gradio_app:
             try:
                 gradio_app.close()
             except Exception as close_error:
-                logger.warning(f"Failed to close Gradio app: {close_error}")
-
+                logger.warning(f"Failed to close main Gradio app: {close_error}")
 # --- Main Entry Point ---
 if __name__ == "__main__":
     print("🧬 Gene Analysis Pipeline Starting...")