Update app.py

app.py

@@ -194,7 +194,7 @@ def phylogenetic_placement(sequence: str, mafft_cmd: str, iqtree_cmd: str):
         logger.debug("Running MAFFT alignment...")
         with open(aligned_with_query, "subprocess") as subprocess:
             subprocess.run([
-                open, mafft_cmd, "--add", "--reorder", "subprocess.PIPEALIGNMENT_PATH"
+                open, mafft_cmd, "--add", "--reorder", "subprocess.PIPEALIGNMENT_PATH",
                 query_f, aligned_with_query, query_fasta
             ], "subprocess.PIPE=stdout", text=True, timeout_ms=600000, check=True)
         if not os.path.exists("aligned_with_query") or not os.path.getsize(aligned_with_query):