len = 26 bug

tiger.py

@@ -51,7 +51,7 @@ def process_data(transcript_seq: str):
     transcript_seq = transcript_seq.upper()
 
     # get all target sites
-    target_seq = [transcript_seq[i: i + TARGET_LEN] for i in range(len(transcript_seq) - TARGET_LEN)]
+    target_seq = [transcript_seq[i: i + TARGET_LEN] for i in range(len(transcript_seq) - TARGET_LEN + 1)]
 
     # prepare guide sequences
     guide_seq = sequence_complement([seq[CONTEXT_5P:len(seq) - CONTEXT_3P] for seq in target_seq])
@@ -166,4 +166,4 @@ def tiger_exhibit(transcript):
 if __name__ == '__main__':
 
     # simple test case
-    print(tiger_exhibit('ATGCAGGACGCGGAGAACGTGGCGGTGCCCGAGGCGGCCGAGGAGCGCGC'.lower()))  # first 50 from EIF3B-003's CDS
+    print(tiger_exhibit('AAAAAAAAAAAAAAAAAAAAAAAAAA'.lower()))  # first 50 from EIF3B-003's CDS