Delete app.py
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app.py
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import gradio as gr
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import spaces
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from cellpose import models
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import numpy as np
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import cv2
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import matplotlib.pyplot as plt
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import tempfile
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from PIL import Image
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import io
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from huggingface_hub import hf_hub_download
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HF_REPO_ID = "myang4218/cellposemodel"
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MODEL_OPTIONS = {
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"Hemocytometer Model": "hemocytometermodel.npy",
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"General Model": "generalmodel.npy"
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}
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loaded_models = {}
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# Global variable for region coordinates (can remain global if only for info display)
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current_region_coords = None
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"""
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# Commented out debugging function
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def debug_editor_data(editor_data):
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\"\"\"Debug function to understand the structure of ImageEditor data\"\"\"
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if editor_data is None:
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return "No data provided"
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info = f"Type: {type(editor_data)}\n"
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if isinstance(editor_data, dict):
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info += f"Keys: {list(editor_data.keys())}\n"
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for key, value in editor_data.items():
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info += f" {key}: {type(value)}\n"
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if key == 'layers' and value:
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info += f" Layers count: {len(value)}\n"
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for i, layer in enumerate(value):
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info += f" Layer {i}: {type(layer)}\n"
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if hasattr(layer, 'size'):
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info += f" Size: {layer.size}\n"
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info += f" Mode: {layer.mode}\n"
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else: # Direct PIL Image
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if hasattr(editor_data, 'size'):
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info += f"Size: {editor_data.size}\n"
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info += f"Mode: {editor_data.mode}\n"
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return info
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"""
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def extract_region_from_editor(editor_data):
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"""Extract the selected region from ImageEditor data"""
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if editor_data is None:
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# print("DEBUG: extract_region_from_editor - editor_data is None")
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return None, None
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# print(f"DEBUG: extract_region_from_editor - editor_data type: {type(editor_data)}")
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if isinstance(editor_data, dict):
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background = editor_data.get('background')
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layers = editor_data.get('layers', [])
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if background is None:
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# print("DEBUG: extract_region_from_editor - background is None")
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return None, None
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background_np = np.array(background)
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# print(f"DEBUG: extract_region_from_editor - background_np shape: {background_np.shape}")
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if layers and len(layers) > 0:
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selection_layer = layers[0]
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selection_np = np.array(selection_layer)
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# print(f"DEBUG: extract_region_from_editor - selection_np shape: {selection_np.shape}")
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if len(selection_np.shape) == 3:
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if selection_np.shape[2] == 4: # RGBA
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mask = selection_np[:, :, 3] > 0
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else: # RGB
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mask = np.any(selection_np > 0, axis=2)
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else:
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mask = selection_np > 0
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coords = np.where(mask)
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if len(coords[0]) > 0:
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y_min, y_max = coords[0].min(), coords[0].max()
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x_min, x_max = coords[1].min(), coords[1].max()
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pad = 5
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h, w = background_np.shape[:2]
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y_min = max(0, y_min - pad)
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y_max = min(h, y_max + pad)
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x_min = max(0, x_min - pad)
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x_max = min(w, x_max + pad)
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region = background_np[y_min:y_max+1, x_min:x_max+1]
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# print(f"DEBUG: extract_region_from_editor - Extracted region shape: {region.shape}")
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return region, (x_min, y_min, x_max, y_max)
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# print("DEBUG: extract_region_from_editor - No selection or empty selection, returning full background.")
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return background_np, None
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else: # Assuming it's a direct PIL Image if not dict
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if hasattr(editor_data, 'size'):
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image_np = np.array(editor_data)
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# print(f"DEBUG: extract_region_from_editor - Direct PIL image, shape: {image_np.shape}")
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return image_np, None
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else:
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# print("DEBUG: extract_region_from_editor - editor_data is not dict and no size attribute, returning None.")
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return None, None
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def calculate_blue_intensity(image_np, masks):
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"""Calculate blue intensity for each cell"""
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# Ensure image is in correct format
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if len(image_np.shape) == 2:
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# Convert grayscale to RGB
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image_np = cv2.cvtColor(image_np, cv2.COLOR_GRAY2RGB)
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elif len(image_np.shape) == 3 and image_np.shape[2] == 4:
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# Handle RGBA
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image_np = cv2.cvtColor(image_np, cv2.COLOR_RGBA2RGB)
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# Calculate blue intensity for each cell
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cell_blue_intensities = {}
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unique_cells = np.unique(masks)
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# print(f"DEBUG: Found {len(unique_cells)} unique mask values: {unique_cells}")
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# print(f"DEBUG: Image shape: {image_np.shape}, dtype: {image_np.dtype}")
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# print(f"DEBUG: Masks shape: {masks.shape}, dtype: {masks.dtype}")
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for cell_id in unique_cells:
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if cell_id == 0: # Skip background
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continue
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cell_mask = masks == cell_id
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cell_pixels = image_np[cell_mask]
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if len(cell_pixels) > 0:
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# Calculate mean blue intensity (blue channel is index 2)
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blue_intensity = np.mean(cell_pixels[:, 2]) # Blue channel
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cell_blue_intensities[cell_id] = blue_intensity
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# print(f"DEBUG: Cell {cell_id}: {len(cell_pixels)} pixels, blue intensity: {blue_intensity:.2f}")
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# print(f"DEBUG: Total cells with blue intensity: {len(cell_blue_intensities)}")
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return cell_blue_intensities
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def create_viability_overlay(image_np, masks, blue_threshold):
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"""Create overlay showing live/dead cells based on blue threshold"""
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if masks is None or image_np is None:
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# print("DEBUG: create_viability_overlay - masks or image_np is None, returning None.")
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return None, 0, 0
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# print(f"DEBUG: create_viability_overlay called with threshold: {blue_threshold}")
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# print(f"DEBUG: Image shape: {image_np.shape}, Masks shape: {masks.shape}, Masks max: {masks.max()}")
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# Ensure image is RGB
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if len(
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