Spaces:
Sleeping
Sleeping
Update app.py
Browse files
app.py
CHANGED
|
@@ -431,72 +431,8 @@ with gr.Blocks(
|
|
| 431 |
inputs=[blue_threshold1, masks_state, image_state],
|
| 432 |
outputs=[viability_overlay1, live_count_output1, dead_count_output1, viability_percent_output1, viability_info1]
|
| 433 |
)
|
| 434 |
-
|
| 435 |
-
with gr.Tab("Manual Coordinates"):
|
| 436 |
-
gr.Markdown("### Upload image and run segmentation")
|
| 437 |
|
| 438 |
-
|
| 439 |
-
with gr.Column():
|
| 440 |
-
image_input = gr.Image(type="pil", label="Microscopy Image")
|
| 441 |
-
model_dropdown2 = gr.Dropdown(
|
| 442 |
-
choices=list(MODEL_OPTIONS.keys()),
|
| 443 |
-
label="Select Model",
|
| 444 |
-
value="Hemocytometer Model"
|
| 445 |
-
)
|
| 446 |
-
coord_input = gr.Textbox(
|
| 447 |
-
label="Crop Coordinates (optional)",
|
| 448 |
-
placeholder="e.g., 100,100,400,400",
|
| 449 |
-
info="Format: x_min,y_min,x_max,y_max"
|
| 450 |
-
)
|
| 451 |
-
segment_btn2 = gr.Button("🔬 Run Segmentation", variant="primary", size="lg")
|
| 452 |
-
|
| 453 |
-
with gr.Column():
|
| 454 |
-
cell_count_output2 = gr.Number(label="Total Cells Detected", precision=0)
|
| 455 |
-
confluency_output2 = gr.Number(label="Confluency (%)", precision=1)
|
| 456 |
-
overlay_output2 = gr.Image(type="pil", label="Segmentation Result")
|
| 457 |
-
info_output2 = gr.Textbox(label="Processing Info", lines=4)
|
| 458 |
-
|
| 459 |
-
# Viability Assessment Section
|
| 460 |
-
with gr.Group(visible=False) as viability_section2:
|
| 461 |
-
gr.Markdown("### Viability Assessment (Trypan Blue)")
|
| 462 |
-
gr.Markdown("Adjust the threshold to classify cells as live (green) or dead (red).")
|
| 463 |
-
|
| 464 |
-
with gr.Row():
|
| 465 |
-
with gr.Column():
|
| 466 |
-
blue_threshold2 = gr.Slider(
|
| 467 |
-
minimum=0,
|
| 468 |
-
maximum=100,
|
| 469 |
-
value=25,
|
| 470 |
-
step=1,
|
| 471 |
-
label="Blue Threshold (%)",
|
| 472 |
-
info="Higher values = more selective for blue cells"
|
| 473 |
-
)
|
| 474 |
-
|
| 475 |
-
with gr.Column():
|
| 476 |
-
live_count_output2 = gr.Number(label="Live Cells (Green)", precision=0)
|
| 477 |
-
dead_count_output2 = gr.Number(label="Dead Cells (Red)", precision=0)
|
| 478 |
-
|
| 479 |
-
viability_overlay2 = gr.Image(type="pil", label="Viability Assessment (Green=Live, Red=Dead)")
|
| 480 |
-
viability_percent_output2 = gr.Number(label="Viability (%)", precision=1)
|
| 481 |
-
viability_info2 = gr.Textbox(label="Analysis Results", lines=5)
|
| 482 |
-
|
| 483 |
-
# Event handlers
|
| 484 |
-
segment_btn2.click(
|
| 485 |
-
fn=run_segmentation_manual,
|
| 486 |
-
inputs=[image_input, model_dropdown2, coord_input],
|
| 487 |
-
outputs=[cell_count_output2, overlay_output2, info_output2, viability_section2, masks_state, image_state, confluency_output2]
|
| 488 |
-
).then( # Chain the initial viability assessment after segmentation
|
| 489 |
-
fn=update_viability_realtime,
|
| 490 |
-
inputs=[blue_threshold2, masks_state, image_state],
|
| 491 |
-
outputs=[viability_overlay2, live_count_output2, dead_count_output2, viability_percent_output2, viability_info2]
|
| 492 |
-
)
|
| 493 |
-
|
| 494 |
-
# Slider changes update viability in real-time
|
| 495 |
-
blue_threshold2.change(
|
| 496 |
-
fn=update_viability_realtime,
|
| 497 |
-
inputs=[blue_threshold2, masks_state, image_state],
|
| 498 |
-
outputs=[viability_overlay2, live_count_output2, dead_count_output2, viability_percent_output2, viability_info2]
|
| 499 |
-
)
|
| 500 |
|
| 501 |
# Instructions
|
| 502 |
with gr.Accordion("Instructions", open=False):
|
|
|
|
| 431 |
inputs=[blue_threshold1, masks_state, image_state],
|
| 432 |
outputs=[viability_overlay1, live_count_output1, dead_count_output1, viability_percent_output1, viability_info1]
|
| 433 |
)
|
|
|
|
|
|
|
|
|
|
| 434 |
|
| 435 |
+
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| 436 |
|
| 437 |
# Instructions
|
| 438 |
with gr.Accordion("Instructions", open=False):
|