import os from Bio import SeqIO from Bio.Seq import Seq def translate_fasta_files(input_folder): """ Translates all DNA FASTA files in the given folder to protein sequences. Args: input_folder (str): Path to the folder containing FASTA files. """ for filename in os.listdir(input_folder): if (filename.endswith(".fasta") or filename.endswith(".fa")) and not filename.endswith("_prot.fasta"): input_path = os.path.join(input_folder, filename) output_path = os.path.join(input_folder, filename.rsplit(".", 1)[0] + "_prot.fasta") print("Input path: ", input_path) with open(output_path, "w") as output_file: for record in SeqIO.parse(input_path, "fasta"): protein_seq = Seq(record.seq).translate(to_stop=True) # Stops at stop codon record.seq = protein_seq SeqIO.write(record, output_file, "fasta") print(f"Translated: {filename} -> {output_path}") if __name__ == "__main__": folder = input("Enter the path to the folder containing FASTA files: ") translate_fasta_files(folder)