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<html lang="zh-CN">
<head>
<meta charset="UTF-8">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<title>qPCR引物设计工具</title>
<style>
* {
margin: 0;
padding: 0;
box-sizing: border-box;
}
body {
font-family: 'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;
background: linear-gradient(135deg, #667eea 0%, #764ba2 100%);
min-height: 100vh;
padding: 20px;
}
.container {
max-width: 1200px;
margin: 0 auto;
background: white;
border-radius: 15px;
box-shadow: 0 20px 40px rgba(0,0,0,0.1);
overflow: hidden;
}
.header {
background: linear-gradient(135deg, #4facfe 0%, #00f2fe 100%);
color: white;
padding: 30px;
text-align: center;
}
.header h1 {
font-size: 2.5em;
margin-bottom: 10px;
}
.header p {
font-size: 1.1em;
opacity: 0.9;
}
.form-section {
padding: 40px;
}
.tab-container {
margin-bottom: 30px;
}
.tab-buttons {
display: flex;
border-bottom: 2px solid #e1e5e9;
margin-bottom: 20px;
}
.tab-button {
padding: 12px 24px;
background: none;
border: none;
cursor: pointer;
font-size: 16px;
font-weight: 600;
color: #666;
border-bottom: 3px solid transparent;
transition: all 0.3s;
}
.tab-button.active {
color: #4facfe;
border-bottom-color: #4facfe;
}
.tab-content {
display: none;
}
.tab-content.active {
display: block;
}
.batch-input {
margin-bottom: 20px;
}
.batch-textarea {
width: 100%;
min-height: 150px;
padding: 15px;
border: 2px solid #e1e5e9;
border-radius: 8px;
font-size: 14px;
font-family: 'Courier New', monospace;
resize: vertical;
}
.batch-help {
background: #f8f9fa;
padding: 15px;
border-radius: 8px;
margin-bottom: 20px;
font-size: 14px;
color: #666;
}
.export-section {
background: #f8f9fa;
padding: 20px;
border-radius: 8px;
margin-top: 20px;
}
.export-buttons {
display: flex;
gap: 10px;
flex-wrap: wrap;
}
.export-btn {
background: #28a745;
color: white;
padding: 10px 20px;
border: none;
border-radius: 6px;
cursor: pointer;
font-size: 14px;
font-weight: 600;
transition: background 0.3s;
}
.export-btn:hover {
background: #218838;
}
.export-btn:disabled {
background: #6c757d;
cursor: not-allowed;
}
.batch-progress {
display: none;
margin: 20px 0;
}
.progress-bar {
width: 100%;
height: 20px;
background: #e1e5e9;
border-radius: 10px;
overflow: hidden;
}
.progress-fill {
height: 100%;
background: linear-gradient(90deg, #4facfe, #00f2fe);
width: 0%;
transition: width 0.3s;
}
.progress-text {
text-align: center;
margin-top: 10px;
font-weight: 600;
}
.batch-results {
display: none;
}
.result-summary {
background: white;
padding: 20px;
border-radius: 8px;
margin-bottom: 20px;
display: flex;
justify-content: space-between;
align-items: center;
}
.summary-stats {
display: flex;
gap: 30px;
}
.stat-item {
text-align: center;
}
.stat-number {
font-size: 24px;
font-weight: bold;
color: #4facfe;
}
.stat-label {
font-size: 12px;
color: #666;
text-transform: uppercase;
}
.failed-genes {
background: #fff3cd;
border: 1px solid #ffeaa7;
padding: 15px;
border-radius: 8px;
margin-bottom: 20px;
}
.failed-gene {
margin-bottom: 10px;
padding: 8px;
background: white;
border-radius: 4px;
border-left: 3px solid #e17055;
}
.form-group {
margin-bottom: 25px;
}
label {
display: block;
margin-bottom: 8px;
font-weight: 600;
color: #333;
}
input, select {
width: 100%;
padding: 12px 15px;
border: 2px solid #e1e5e9;
border-radius: 8px;
font-size: 16px;
transition: border-color 0.3s;
}
input:focus, select:focus {
outline: none;
border-color: #4facfe;
}
.btn {
background: linear-gradient(135deg, #667eea 0%, #764ba2 100%);
color: white;
padding: 15px 30px;
border: none;
border-radius: 8px;
font-size: 16px;
font-weight: 600;
cursor: pointer;
transition: transform 0.2s;
width: 100%;
}
.btn:hover {
transform: translateY(-2px);
}
.btn:disabled {
opacity: 0.6;
cursor: not-allowed;
transform: none;
}
.loading {
display: none;
text-align: center;
padding: 20px;
}
.spinner {
border: 4px solid #f3f3f3;
border-top: 4px solid #4facfe;
border-radius: 50%;
width: 40px;
height: 40px;
animation: spin 1s linear infinite;
margin: 0 auto 10px;
}
@keyframes spin {
0% { transform: rotate(0deg); }
100% { transform: rotate(360deg); }
}
.results {
display: none;
padding: 40px;
background: #f8f9fa;
}
.gene-info {
background: white;
padding: 20px;
border-radius: 8px;
margin-bottom: 30px;
border-left: 4px solid #4facfe;
}
.primer-card {
background: white;
border-radius: 8px;
padding: 20px;
margin-bottom: 20px;
box-shadow: 0 2px 10px rgba(0,0,0,0.1);
}
.primer-header {
display: flex;
justify-content: space-between;
align-items: center;
margin-bottom: 15px;
}
.primer-id {
background: #4facfe;
color: white;
padding: 5px 15px;
border-radius: 20px;
font-weight: 600;
}
.product-size {
background: #e8f5e8;
color: #2d5a2d;
padding: 5px 15px;
border-radius: 20px;
font-weight: 600;
}
.primer-sequences {
display: grid;
grid-template-columns: 1fr 1fr;
gap: 20px;
margin-bottom: 15px;
}
.sequence-box {
background: #f8f9fa;
padding: 15px;
border-radius: 6px;
border-left: 3px solid #4facfe;
}
.sequence-label {
font-weight: 600;
color: #666;
margin-bottom: 5px;
}
.sequence {
font-family: 'Courier New', monospace;
font-size: 14px;
word-break: break-all;
background: white;
padding: 8px;
border-radius: 4px;
border: 1px solid #e1e5e9;
}
.tm-info {
display: flex;
justify-content: space-between;
font-size: 14px;
color: #666;
}
.error {
background: #fee;
color: #c33;
padding: 15px;
border-radius: 8px;
border-left: 4px solid #c33;
margin: 20px 0;
}
@media (max-width: 768px) {
.primer-sequences {
grid-template-columns: 1fr;
}
.primer-header {
flex-direction: column;
gap: 10px;
}
}
</style>
</head>
<body>
<div class="container">
<div class="header">
<h1>🧬 qPCR引物设计工具</h1>
<p>输入基因名称和物种，自动设计跨越外显子交界点的qPCR引物</p>
<p style="margin-top: 15px; font-size: 0.9em; opacity: 0.8;">Developed by PharC | Contact: pc794797023@163.com</p>
</div>
<div class="form-section">
<div class="tab-container">
<div class="tab-buttons">
<button type="button" class="tab-button active" onclick="switchTab('single')">
🧬 单个基因
</button>
<button type="button" class="tab-button" onclick="switchTab('batch')">
📋 批量处理
</button>
</div>
<!-- 单个基因设计 -->
<div id="single-tab" class="tab-content active">
<form id="primerForm">
<div class="form-group">
<label for="geneSymbol">基因名称 *</label>
<input type="text" id="geneSymbol" name="geneSymbol" placeholder="例如: GAPDH, Gpr34" required>
</div>
<div class="form-group">
<label for="species">物种</label>
<select id="species" name="species">
<option value="human">人类 (Human)</option>
<option value="Mus musculus">小鼠 (Mouse)</option>
<option value="Rattus norvegicus">大鼠 (Rat)</option>
<option value="Drosophila melanogaster">果蝇 (Drosophila)</option>
<option value="Caenorhabditis elegans">线虫 (C. elegans)</option>
<option value="Danio rerio">斑马鱼 (Zebrafish)</option>
</select>
</div>
<button type="submit" class="btn" id="submitBtn">
🔬 设计引物
</button>
</form>
</div>
<!-- 批量基因设计 -->
<div id="batch-tab" class="tab-content">
<form id="batchForm">
<div class="batch-help">
💡 <strong>使用说明：</strong>每行输入一个基因名称，支持多种格式：
<br>• 一行一个基因：GAPDH
<br>• 逗号分隔：GAPDH, ACTB, TUBB3
<br>• 空格分隔：GAPDH ACTB TUBB3
</div>
<div class="form-group">
<label for="batchGenes">基因列表 *</label>
<textarea
id="batchGenes"
class="batch-textarea"
placeholder="请输入基因名称，每行一个或用逗号/空格分隔： GAPDH ACTB TUBB3 或者：GAPDH, ACTB, TUBB3"
required
></textarea>
</div>
<div class="form-group">
<label for="batchSpecies">物种</label>
<select id="batchSpecies" name="batchSpecies">
<option value="human">人类 (Human)</option>
<option value="Mus musculus">小鼠 (Mouse)</option>
<option value="Rattus norvegicus">大鼠 (Rat)</option>
<option value="Drosophila melanogaster">果蝇 (Drosophila)</option>
<option value="Caenorhabditis elegans">线虫 (C. elegans)</option>
<option value="Danio rerio">斑马鱼 (Zebrafish)</option>
</select>
</div>
<button type="submit" class="btn" id="batchSubmitBtn">
🚀 批量设计引物
</button>
</form>
<div class="batch-progress" id="batchProgress">
<div class="progress-bar">
<div class="progress-fill" id="progressFill"></div>
</div>
<div class="progress-text" id="progressText">正在处理: 0/0</div>
</div>
</div>
</div>
</div>
<div class="loading" id="loading">
<div class="spinner"></div>
<p>正在设计引物，请稍候...</p>
</div>
<div class="results" id="results">
<div class="gene-info" id="geneInfo"></div>
<div id="primerResults"></div>
<div class="export-section" id="exportSection" style="display: none;">
<h4>📥 导出结果</h4>
<p>选择导出格式：</p>
<div class="export-buttons">
<button class="export-btn" onclick="exportResults('excel')">
📊 Excel格式
</button>
<button class="export-btn" onclick="exportResults('csv')">
📄 CSV格式
</button>
<button class="export-btn" onclick="exportResults('json')">
🔧 JSON格式
</button>
</div>
</div>
</div>
<div class="batch-results" id="batchResults">
<div class="result-summary" id="resultSummary"></div>
<div id="failedGenes"></div>
<div id="batchPrimerResults"></div>
<div class="export-section">
<h4>📥 导出批量结果</h4>
<p>选择导出格式：</p>
<div class="export-buttons">
<button class="export-btn" onclick="exportBatchResults('excel')">
📊 Excel格式
</button>
<button class="export-btn" onclick="exportBatchResults('csv')">
📄 CSV格式
</button>
<button class="export-btn" onclick="exportBatchResults('json')">
🔧 JSON格式
</button>
</div>
</div>
</div>
</div>
<script src="{{ url_for('static', filename='app.js') }}"></script>
</body>
</html> |