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import json
import os
from pathlib import Path
import numpy as np
# Path to RAG knowledge files (JSON and TXT files with brain region descriptions)
RAG_KNOWLEDGE_DIR = Path(__file__).resolve().parent.parent / "data" / "rag_knowledge"
def load_knowledge_from_files(directory: Path | None = None) -> dict[str, str]:
"""Load brain region knowledge from user-supplied JSON and TXT files.
JSON files: {"Region Name": "description", ...}
TXT files: sections separated by '## Region Name' headers.
Returns merged dict. Empty dict if directory missing/empty.
"""
directory = directory or RAG_KNOWLEDGE_DIR
knowledge: dict[str, str] = {}
if not directory.is_dir():
return knowledge
# Process files in sorted order for deterministic merging
for fpath in sorted(directory.iterdir()):
if fpath.suffix == ".json":
try:
data = json.loads(fpath.read_text(encoding="utf-8"))
if isinstance(data, dict):
count = 0
for k, v in data.items():
if isinstance(k, str) and isinstance(v, str):
knowledge[k] = v
count += 1
if count:
print(f"[RAG] Loaded {count} regions from {fpath.name}")
except Exception as e:
print(f"[RAG] Warning: could not load {fpath.name}: {e}")
elif fpath.suffix == ".txt":
try:
text = fpath.read_text(encoding="utf-8")
current_region = None
current_lines: list[str] = []
count = 0
for line in text.splitlines():
if line.startswith("## "):
if current_region and current_lines:
knowledge[current_region] = " ".join(current_lines).strip()
count += 1
current_region = line[3:].strip()
current_lines = []
elif current_region is not None:
current_lines.append(line.strip())
if current_region and current_lines:
knowledge[current_region] = " ".join(current_lines).strip()
count += 1
if count:
print(f"[RAG] Loaded {count} regions from {fpath.name}")
except Exception as e:
print(f"[RAG] Warning: could not load {fpath.name}: {e}")
return knowledge
def _call_direct_responses_api(question: str, model: str) -> str:
"""Direct OpenAI Responses API call for no-RAG mode.
Avoids the LangChain empty-content failure path seen with GPT-5.4 in the
background thread.
"""
_ensure_ssl()
from dotenv import load_dotenv
load_dotenv()
from openai import OpenAI
client = OpenAI(timeout=60.0)
instructions = (
"You are a neuroscience expert interpreting information flow in a "
"resting-state brain.\n\n"
"Do NOT repeat the region list.\n"
"Do explain:\n"
"1. what specific information is likely flowing,\n"
"2. how the signal is transformed across regions,\n"
"3. what spontaneous resting-state process could produce this path,\n"
"4. the functional purpose of this exact trajectory.\n"
"Be concrete, not generic."
)
resp = client.responses.create(
model=model,
instructions=instructions,
input=question,
# This is not a model downgrade. It just keeps enough budget for the visible answer.
reasoning={"effort": "low"},
max_output_tokens=2500,
# Optional, if your SDK/version accepts it and you want longer answers:
# text={"verbosity": "high"},
)
text = (resp.output_text or "").strip()
if text:
return text
if getattr(resp, "status", None) == "incomplete" and getattr(resp, "incomplete_details", None):
reason = getattr(resp.incomplete_details, "reason", "unknown")
return f"[GPT ERROR] Responses API incomplete: {reason}"
return "[GPT ERROR] Responses API returned no visible text."
def _get_mesh_volume(mesh_overlay, key: str) -> float:
"""Estimate mesh volume from bounding box (for nesting detection)."""
bounds = mesh_overlay.get_mesh_bounds(key)
if bounds is None:
return float('inf')
return float((bounds[1] - bounds[0]) * (bounds[3] - bounds[2]) * (bounds[5] - bounds[4]))
def analyze_probe_path(path_positions: np.ndarray, mesh_overlay,
sample_every: int = 10,
field_mags: np.ndarray | None = None,
entropy_sampler=None,
entropy_field=None) -> list[dict]:
"""Analyze which brain regions the probe traveled through.
Handles nested regions: when the probe is inside multiple overlapping
regions (e.g., a subregion inside a larger area), the smaller region
is treated as the primary region and the larger one as context.
Args:
path_positions: (N,3) probe path
mesh_overlay: FlowMeshOverlay instance
sample_every: check every N steps
field_mags: raw field magnitudes (independent of speed scale)
entropy_sampler: TriLinearSampler for entropy
entropy_field: entropy grid (G,G,G) numpy array
Returns a list of dicts with keys: region_key, region_name, entry_idx,
exit_idx, relative_position, avg_flow_strength, avg_entropy,
enclosing_regions.
"""
if len(path_positions) == 0:
return []
region_keys = mesh_overlay.get_all_region_keys()
# Pre-compute volumes for nesting detection
volumes = {k: _get_mesh_volume(mesh_overlay, k) for k in region_keys}
transitions = []
prev_regions = set()
for i in range(0, len(path_positions), sample_every):
pt = path_positions[i]
in_regions = set()
for key in region_keys:
if mesh_overlay.point_in_mesh(pt, key):
in_regions.add(key)
# Sample entropy at this point
ent_val = None
if entropy_sampler is not None and entropy_field is not None:
try:
ent_val = float(entropy_sampler.sample_scalar(entropy_field, pt[None, :])[0])
except Exception:
pass
# Detect entries
entered = in_regions - prev_regions
for key in entered:
center = mesh_overlay.get_mesh_center(key)
rel_pos = _relative_position(pt, center, mesh_overlay.get_mesh_bounds(key))
# Find enclosing (larger) regions at this point
enclosing = []
for other_key in in_regions:
if other_key != key and volumes.get(other_key, 0) > volumes.get(key, 0):
enclosing.append(mesh_overlay.get_region_name(other_key))
# Flow strength (independent of speed scale)
flow_str = None
if field_mags is not None and i < len(field_mags):
flow_str = float(field_mags[i])
# Hemisphere info (if mesh_overlay supports it)
hemisphere = None
if hasattr(mesh_overlay, 'get_hemisphere_label'):
try:
hemisphere = mesh_overlay.get_hemisphere_label(key, pt)
except Exception:
pass
transition_dict = {
"region_key": key,
"region_name": mesh_overlay.get_region_name(key),
"entry_idx": i,
"exit_idx": None,
"relative_position": rel_pos,
"entry_point": pt.copy(),
"enclosing_regions": enclosing,
"avg_flow_strength": flow_str,
"avg_entropy": ent_val,
"_flow_samples": [flow_str] if flow_str is not None else [],
"_ent_samples": [ent_val] if ent_val is not None else [],
}
if hemisphere is not None:
transition_dict["hemisphere"] = hemisphere
# Extra parcellation subregion info
if hasattr(mesh_overlay, '_extra') and mesh_overlay._extra is not None:
sub = mesh_overlay._extra.get_region_at_point(pt)
if sub is not None:
transition_dict["subregion_name"] = sub["name"]
transition_dict["subregion_volume"] = sub["volume_mm3"]
transitions.append(transition_dict)
# Accumulate samples for active transitions
for t in transitions:
if t["exit_idx"] is None:
if field_mags is not None and i < len(field_mags):
t["_flow_samples"].append(float(field_mags[i]))
if ent_val is not None:
t["_ent_samples"].append(ent_val)
# Detect exits
exited = prev_regions - in_regions
for key in exited:
for t in reversed(transitions):
if t["region_key"] == key and t["exit_idx"] is None:
t["exit_idx"] = i
if t["_flow_samples"]:
t["avg_flow_strength"] = float(np.mean(t["_flow_samples"]))
if t["_ent_samples"]:
t["avg_entropy"] = float(np.mean(t["_ent_samples"]))
break
prev_regions = in_regions
# Close any still-open regions
for t in transitions:
if t["exit_idx"] is None:
t["exit_idx"] = len(path_positions) - 1
if t["_flow_samples"]:
t["avg_flow_strength"] = float(np.mean(t["_flow_samples"]))
if t["_ent_samples"]:
t["avg_entropy"] = float(np.mean(t["_ent_samples"]))
# Pre-compute sampled path points for nearby detection / enrichment
sampled_pts = path_positions[::sample_every]
# --- Enrich each transition with detailed interaction context ---
for t in transitions:
key = t["region_key"]
entry_i = t["entry_idx"]
exit_i = t["exit_idx"] if t["exit_idx"] is not None else len(path_positions) - 1
center = mesh_overlay.get_mesh_center(key)
bounds = mesh_overlay.get_mesh_bounds(key)
# Collect all path points inside this region's span
inside_pts = path_positions[entry_i:exit_i + 1:sample_every]
# 1. Interaction type: how deeply the probe penetrated
if center is not None and bounds is not None and len(inside_pts) > 0:
extent = np.array([bounds[1]-bounds[0], bounds[3]-bounds[2],
bounds[5]-bounds[4]])
extent = np.maximum(extent, 1e-6)
char_size = float(np.mean(extent)) # characteristic region size
# Distance from each inside point to region center
dists_to_center = np.linalg.norm(inside_pts - center, axis=1)
min_dist = float(dists_to_center.min())
mean_dist = float(dists_to_center.mean())
# Normalized penetration depth (0 = at center, 1 = at edge)
penetration = 1.0 - min(min_dist / (char_size * 0.5), 1.0)
if penetration > 0.6:
interaction = "passed through center"
elif penetration > 0.3:
interaction = "traversed mid-region"
elif len(inside_pts) <= 2:
interaction = "briefly touched surface"
else:
interaction = "passed through periphery"
t["interaction_type"] = interaction
t["penetration_depth"] = round(penetration, 2)
else:
t["interaction_type"] = "traversed"
t["penetration_depth"] = None
# 2. Exit position (relative to region)
if exit_i < len(path_positions) and center is not None and bounds is not None:
exit_pt = path_positions[min(exit_i, len(path_positions) - 1)]
t["exit_position"] = _relative_position(exit_pt, center, bounds)
else:
t["exit_position"] = "unknown"
# 3. Traversal direction through the region
if len(inside_pts) >= 2:
direction_vec = inside_pts[-1] - inside_pts[0]
norm = np.linalg.norm(direction_vec)
if norm > 1e-6:
d = direction_vec / norm
dir_parts = []
if abs(d[2]) > 0.3:
dir_parts.append("upward" if d[2] > 0 else "downward")
if abs(d[0]) > 0.3:
dir_parts.append("rightward" if d[0] > 0 else "leftward")
if abs(d[1]) > 0.3:
dir_parts.append("anteriorly" if d[1] > 0 else "posteriorly")
t["traversal_direction"] = " and ".join(dir_parts) if dir_parts else "through"
else:
t["traversal_direction"] = "stationary"
else:
t["traversal_direction"] = "brief"
# --- Detect nearby regions (probe flowed close but never entered) ---
entered_keys = {t["region_key"] for t in transitions}
NEARBY_THRESHOLD_MM = 5.0 # max distance to consider "nearby"
nearby_transitions = []
for key in region_keys:
if key in entered_keys:
continue
center = mesh_overlay.get_mesh_center(key)
bounds = mesh_overlay.get_mesh_bounds(key)
if center is None or bounds is None:
continue
extent = np.array([bounds[1]-bounds[0], bounds[3]-bounds[2],
bounds[5]-bounds[4]])
extent = np.maximum(extent, 1e-6)
char_size = float(np.mean(extent))
# Check minimum distance from any sampled path point to region center
dists = np.linalg.norm(sampled_pts - center, axis=1)
min_dist = float(dists.min())
# "nearby" = within threshold but also within the region's own characteristic size
effective_radius = max(char_size * 0.5, NEARBY_THRESHOLD_MM)
if min_dist < effective_radius:
closest_idx = int(dists.argmin()) * sample_every
name = mesh_overlay.get_region_name(key)
hemisphere = None
if hasattr(mesh_overlay, 'get_hemisphere_label'):
try:
hemisphere = mesh_overlay.get_hemisphere_label(
key, sampled_pts[int(dists.argmin())])
except Exception:
pass
nearby_t = {
"region_key": key,
"region_name": name,
"entry_idx": closest_idx,
"exit_idx": closest_idx,
"relative_position": _relative_position(
sampled_pts[int(dists.argmin())], center, bounds),
"entry_point": sampled_pts[int(dists.argmin())].copy(),
"enclosing_regions": [],
"avg_flow_strength": None,
"avg_entropy": None,
"interaction_type": f"nearby (did not enter, ~{min_dist:.1f}mm away)",
"penetration_depth": 0.0,
"exit_position": "n/a",
"traversal_direction": "n/a",
}
if hemisphere is not None:
nearby_t["hemisphere"] = hemisphere
nearby_transitions.append(nearby_t)
transitions.extend(nearby_transitions)
# Clean up internal fields
for t in transitions:
t.pop("_flow_samples", None)
t.pop("_ent_samples", None)
# Sort by volume (smaller = more specific) so primary regions come first at each transition
transitions.sort(key=lambda t: (t["entry_idx"], volumes.get(t["region_key"], float('inf'))))
return transitions
def _relative_position(point: np.ndarray, center: np.ndarray | None,
bounds: np.ndarray | None) -> str:
"""Describe position relative to region center (e.g., 'upper-left')."""
if center is None or bounds is None:
return "unknown"
diff = point - center
extent = np.array([
bounds[1] - bounds[0],
bounds[3] - bounds[2],
bounds[5] - bounds[4],
])
extent = np.maximum(extent, 1e-6)
rel = diff / (extent * 0.5) # normalized to [-1, 1]
parts = []
if abs(rel[2]) > 0.3:
parts.append("upper" if rel[2] > 0 else "lower")
if abs(rel[0]) > 0.3:
parts.append("right" if rel[0] > 0 else "left")
if abs(rel[1]) > 0.3:
parts.append("anterior" if rel[1] > 0 else "posterior")
if not parts:
return "center"
return "-".join(parts)
def format_transitions_text(transitions: list[dict],
include_branches: list[dict] | None = None) -> str:
"""Format transitions into a human-readable description for GPT.
Handles nested regions: smaller (more specific) regions are primary,
with larger enclosing regions noted as context.
"""
if not transitions:
return "The probe did not pass through any identified brain regions."
lines = ["The probe traveled through the following brain regions in order:\n"]
for i, t in enumerate(transitions, 1):
duration = (t["exit_idx"] or 0) - (t["entry_idx"] or 0)
# Include hemisphere in region name if available
region_label = t['region_name']
hemisphere = t.get("hemisphere")
if hemisphere:
region_label = f"{region_label} ({hemisphere})"
extras = []
if t.get("avg_flow_strength") is not None:
extras.append(f"flow_strength={t['avg_flow_strength']:.4f}")
if t.get("avg_entropy") is not None:
extras.append(f"model_entropy={t['avg_entropy']:.3f} "
f"({'high uncertainty' if t['avg_entropy'] > 1.5 else 'confident'})")
extras_str = f", {', '.join(extras)}" if extras else ""
enclosing = t.get("enclosing_regions", [])
ctx_str = ""
if enclosing:
ctx_str = f" [within larger region: {', '.join(enclosing)}]"
# Extra parcellation subregion info
subregion = t.get("subregion_name")
sub_str = ""
if subregion:
sub_str = f", more specifically in subregion: {subregion}"
# Interaction detail from enrichment
interaction = t.get("interaction_type", "traversed")
is_nearby = "nearby" in interaction
if is_nearby:
# Nearby region: compact format, no penetration/direction/exit noise
entry_pos = t.get("relative_position", "unknown")
pos_str = f" near {entry_pos} section" if entry_pos and entry_pos != "unknown" else ""
lines.append(
f"{i}. {region_label} β {interaction}{pos_str}{ctx_str}{sub_str}"
)
else:
penetration = t.get("penetration_depth")
direction = t.get("traversal_direction", "")
exit_pos = t.get("exit_position", "")
# Build detailed description
detail_parts = [f"{interaction}"]
if penetration is not None:
detail_parts.append(f"penetration depth {penetration:.0%}")
if direction and direction not in ("brief", "stationary", "through", "n/a"):
detail_parts.append(f"moving {direction}")
detail_str = ", ".join(detail_parts)
entry_pos = t.get("relative_position", "unknown")
exit_str = f", exited from {exit_pos}" if exit_pos and exit_pos not in ("unknown", "n/a") else ""
lines.append(
f"{i}. {region_label} β {detail_str}. "
f"Entered at {entry_pos}{exit_str}, "
f"~{duration} steps inside{extras_str}{ctx_str}{sub_str}"
)
if include_branches:
lines.append("\nBranching events (alternative flow paths detected):")
for b in include_branches:
lines.append(f" - Branch at step ~{b.get('entry_idx', '?')}: "
f"flow split toward {b.get('region_name', 'unknown')}")
return "\n".join(lines)
def build_rag_documents():
"""Build LangChain documents from the RAG knowledge base.
Loads all JSON and TXT files from data/rag_knowledge/.
If no files are found, the RAG chain will have no context and the
LLM will rely on its own training knowledge.
"""
from langchain_core.documents import Document
knowledge = load_knowledge_from_files()
if not knowledge:
print("[RAG] Warning: no knowledge files found in data/rag_knowledge/. "
"LLM will use its own training knowledge only.")
docs = []
for name, desc in knowledge.items():
docs.append(Document(
page_content=f"Brain Region: {name}\nFunction: {desc}",
metadata={"region_name": name}
))
return docs
def _ensure_ssl():
"""Fix SSL cert path for environments where Git overrides it."""
try:
import certifi
cert_file = certifi.where()
# Force override if current path is missing (Git installer on Windows
# often sets SSL_CERT_FILE to a non-existent path)
cur = os.environ.get("SSL_CERT_FILE", "")
if not cur or not os.path.isfile(cur):
os.environ["SSL_CERT_FILE"] = cert_file
cur2 = os.environ.get("REQUESTS_CA_BUNDLE", "")
if not cur2 or not os.path.isfile(cur2):
os.environ["REQUESTS_CA_BUNDLE"] = cert_file
except ImportError:
pass
def create_rag_chain(model: str = "gpt-5.4-mini"):
"""Create a LangChain RAG chain with region knowledge."""
_ensure_ssl()
from dotenv import load_dotenv
load_dotenv()
from langchain_openai import ChatOpenAI, OpenAIEmbeddings
from langchain_community.vectorstores import FAISS
from langchain_core.prompts import ChatPromptTemplate
from langchain_core.runnables import RunnablePassthrough
from langchain_core.output_parsers import StrOutputParser
docs = build_rag_documents()
embeddings = OpenAIEmbeddings(model="text-embedding-3-small")
vectorstore = FAISS.from_documents(docs, embeddings)
retriever = vectorstore.as_retriever(search_kwargs={"k": 10})
prompt = ChatPromptTemplate.from_template(
"""You are a neuroscience expert interpreting information flow in a resting-state brain.
Reference knowledge about relevant brain regions:
{context}
{question}
CRITICAL INSTRUCTIONS β read carefully before answering:
1. Do NOT list which regions the probe passed through β the user already knows that.
2. Do NOT give a generic answer like "this flow is associated with the default mode network" β that is too shallow.
3. DO explain what SPECIFIC INFORMATION is likely flowing along this exact path. What is being computed, processed, or communicated? How does the information CHANGE as it passes through each region?
4. DO describe the functional MEANING of this particular flow trajectory: why would information flow in this exact direction, through these specific regions, in this order? What is the computational purpose?
5. DO explain how each region transforms the signal before passing it on β not just what each region "does" in general, but what it does to THIS specific incoming signal.
6. DO consider laterality: if the flow is in the left or right hemisphere, explain what that implies about the type of processing (e.g., left-lateralized language processing, right-lateralized spatial processing).
7. This is a RESTING-STATE brain β describe what spontaneous cognitive process would produce this exact flow. Be concrete: "the brain is likely consolidating a recent spatial memory" is better than "this involves memory processing."
Note: the flow direction reflects rDCM effective connectivity (who influences whom), not neural signal timing. Particle speed is a visualization scale, not biological latency.
"""
)
llm = ChatOpenAI(model=model, temperature=0.3, max_tokens=1500)
def format_docs(docs):
return "\n\n".join(doc.page_content for doc in docs)
chain = (
{"context": retriever | format_docs, "question": RunnablePassthrough()}
| prompt
| llm
| StrOutputParser()
)
return chain
def create_direct_chain(model: str = "gpt-5.4-mini"):
"""Create a direct GPT chain without RAG (uses only GPT's own knowledge)."""
_ensure_ssl()
from dotenv import load_dotenv
load_dotenv()
from langchain_openai import ChatOpenAI
from langchain_core.prompts import ChatPromptTemplate
from langchain_core.runnables import RunnablePassthrough
from langchain_core.output_parsers import StrOutputParser
prompt = ChatPromptTemplate.from_template(
"""You are a neuroscience expert interpreting information flow in a resting-state brain.
Using your deep knowledge of neuroanatomy, functional connectivity, and resting-state \
networks, answer the following question:
{question}
CRITICAL INSTRUCTIONS β read carefully before answering:
1. Do NOT list which regions the probe passed through β the user already knows that.
2. Do NOT give a generic answer like "this flow is associated with the default mode network" β that is too shallow.
3. DO explain what SPECIFIC INFORMATION is likely flowing along this exact path. What is being computed, processed, or communicated? How does the information CHANGE as it passes through each region?
4. DO describe the functional MEANING of this particular flow trajectory: why would information flow in this exact direction, through these specific regions, in this order? What is the computational purpose?
5. DO explain how each region transforms the signal before passing it on β not just what each region "does" in general, but what it does to THIS specific incoming signal.
6. DO consider laterality: if the flow is in the left or right hemisphere, explain what that implies about the type of processing (e.g., left-lateralized language processing, right-lateralized spatial processing).
7. This is a RESTING-STATE brain β describe what spontaneous cognitive process would produce this exact flow. Be concrete: "the brain is likely consolidating a recent spatial memory" is better than "this involves memory processing."
Note: the flow direction reflects rDCM effective connectivity (who influences whom), not neural signal timing. Particle speed is a visualization scale, not biological latency.
"""
)
llm = ChatOpenAI(model=model, temperature=0.3, max_tokens=1500)
chain = (
{"question": RunnablePassthrough()}
| prompt
| llm
| StrOutputParser()
)
return chain
_rag_chain = None
_direct_chain = None
_cached_model = None
def analyze_with_gpt(transitions: list[dict], use_rag: bool = True,
model: str = "gpt-5.4-mini",
debug: bool = False) -> str:
global _rag_chain, _direct_chain, _cached_model
if not transitions:
return "No brain regions were traversed. Place the probe inside the brain volume."
description = format_transitions_text(transitions)
question = (
f"A probe was placed in a resting-state brain and followed the intrinsic information "
f"flow field through these regions:\n\n{description}\n\n"
f"Analyze this SPECIFIC flow pathway in depth:\n"
f"1. What concrete information is likely being carried along this path?\n"
f"2. How does the information CHANGE and get TRANSFORMED as it passes through each region?\n"
f"3. What spontaneous resting-state cognitive process would produce this EXACT flow?\n"
f"4. What is the functional PURPOSE of information flowing in this exact direction and order?\n"
f"Do NOT repeat the region list or give a generic network label. Give deep, specific insight.\n"
f"Keep your response SHORT and focused β 2-3 concise paragraphs maximum."
)
if debug:
import sys
print(f"\n{'='*60}")
print(f"[DEBUG PROMPT] analyze_with_gpt (use_rag={use_rag}, model={model})")
print(f"{'='*60}")
print(question)
print(f"{'='*60}\n")
sys.stdout.flush()
# IMPORTANT: bypass LangChain entirely for no-RAG mode
if not use_rag:
try:
print(f"[GPT] Using Responses API directly (model={model}, no RAG)...")
return _call_direct_responses_api(question, model)
except Exception as e:
import traceback
traceback.print_exc()
return f"[GPT ERROR] Direct Responses API failed: {e}"
# keep existing RAG path for now
if _cached_model != model:
_rag_chain = None
_direct_chain = None
_cached_model = model
if _rag_chain is None:
print(f"[GPT] Initializing RAG chain (model={model})...")
try:
_rag_chain = create_rag_chain(model=model)
except Exception as e:
return f"[GPT ERROR] Failed to initialize RAG: {e}"
try:
result = _rag_chain.invoke(question)
text = result if isinstance(result, str) else str(result)
return text.strip() if text and text.strip() else "[GPT ERROR] Empty RAG response."
except Exception as e:
import traceback
traceback.print_exc()
return f"[GPT ERROR] {e}"
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